npj Biological Timing and Sleep最新文献

The molecular clock drives motivated locomotion and time-of-day-dependent firing patterns in mouse dopaminergic neurons. 分子钟驱动小鼠多巴胺能神经元的动机运动和依赖于时间的放电模式。

npj Biological Timing and Sleep Pub Date : 2025-01-01 Epub Date: 2025-07-03 DOI: 10.1038/s44323-025-00044-2

Ananya Swaroop, Jodi R Paul, Laura J McMeekin, Ashley M Barnett, Alana M Colafrancesco, Drèson L Russell, Camille M Smith, Micah S Simmons, Laura A Volpicelli-Daley, Rita M Cowell, Karen L Gamble

{"title":"The molecular clock drives motivated locomotion and time-of-day-dependent firing patterns in mouse dopaminergic neurons.","authors":"Ananya Swaroop, Jodi R Paul, Laura J McMeekin, Ashley M Barnett, Alana M Colafrancesco, Drèson L Russell, Camille M Smith, Micah S Simmons, Laura A Volpicelli-Daley, Rita M Cowell, Karen L Gamble","doi":"10.1038/s44323-025-00044-2","DOIUrl":"10.1038/s44323-025-00044-2","url":null,"abstract":"Though circadian locomotor rhythms are primarily driven by the suprachiasmatic nucleus, voluntary motor behavior also requires dopaminergic neuron (DAN) activity. However, it is unknown whether DAN molecular and electrophysiological properties and rhythmic motor behaviors are dependent on a molecular clock. Here, we show substantia nigra pars compacta (SNc) and ventral tegmental area (VTA) DANs rhythmically express clock genes, and conditional deletion of Bmal1 in DANs reduces motivated locomotion without robust cell loss or gross motor impairment. Further, DAN Bmal1 conditional deletion disrupts 24-h rhythms in spike rate, revealing ultradian rhythms (~4-8 h). Lastly, SNc DAN bursting varies across time of day and increased early night bursting is dependent on the molecular clock and L-type calcium channel activation. Collectively, we provide evidence of a cell-intrinsic dopaminergic clock which regulates key behaviors and physiology. Future studies should consider the contribution of disrupted DAN molecular clocks in age-related motor diseases like Parkinson's Disease.","PeriodicalId":501704,"journal":{"name":"npj Biological Timing and Sleep","volume":"2 1","pages":"28"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12226335/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144577537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulating light level patterns reduces rest/activity disruption associated with shiftwork. 调节光照模式可减少轮班工作对休息/活动的干扰。

npj Biological Timing and Sleep Pub Date : 2025-01-01 Epub Date: 2025-07-03 DOI: 10.1038/s44323-025-00043-3

Varun Mandi, Haley Miller, Jeongkyung Lee, Young-Hwa Goo, Mousumi Moulik, Ke Ma, Antoni Paul, Vijay K Yechoor, Mariana G Figueiro

{"title":"Modulating light level patterns reduces rest/activity disruption associated with shiftwork.","authors":"Varun Mandi, Haley Miller, Jeongkyung Lee, Young-Hwa Goo, Mousumi Moulik, Ke Ma, Antoni Paul, Vijay K Yechoor, Mariana G Figueiro","doi":"10.1038/s44323-025-00043-3","DOIUrl":"10.1038/s44323-025-00043-3","url":null,"abstract":"Long-term exposure to nonstandard work schedules can result in circadian misalignment, which has been linked to a series of maladies. To test whether modulating light patterns reduces shiftwork-induced rest/activity disruptions, 30 male C57BL/6 J mice individually housed in cages outfitted with running wheels were exposed to 6 simulated shiftwork light interventions. Mice experiencing high light levels during shiftwork exhibited a significant decrease in activity compared to low light levels during shiftwork and a conventional 12 L:12D condition, indicating circadian misalignment. In contrast, mice experiencing shiftwork in darkness combined with either modulated evening light pulses or circadian blind, vision-permissive light showed similar levels of rest/activity compared to a 12 L:12D condition, with phasor analysis indicating that their 24-h circadian rest/activity patterns were not misaligned. The results show that exposure to light that permits visibility but is below activation of the circadian system during shiftwork can prevent circadian misalignment.","PeriodicalId":501704,"journal":{"name":"npj Biological Timing and Sleep","volume":"2 1","pages":"27"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12226348/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144577536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological rhythms: Living your life, one half-day at a time. 生物节奏：过好你的生活，一次过半天。

npj Biological Timing and Sleep Pub Date : 2025-01-01 Epub Date: 2025-06-03 DOI: 10.1038/s44323-025-00037-1

Patrick Emery, Frédéric Gachon

引用次数: 0

The complexity and commonness of the two-process model of sleep regulation from a mathematical perspective. 从数学角度看睡眠调节双过程模型的复杂性与共性。

npj Biological Timing and Sleep Pub Date : 2025-01-01 Epub Date: 2025-06-18 DOI: 10.1038/s44323-025-00039-z

Anne C Skeldon, Derk-Jan Dijk

引用次数: 0

Revisiting brain gene expression changes and protein modifications tracking homeostatic sleep pressure. 重新审视大脑基因表达变化和蛋白质修饰跟踪稳态睡眠压力。

npj Biological Timing and Sleep Pub Date : 2025-01-01 Epub Date: 2025-08-04 DOI: 10.1038/s44323-025-00045-1

Valérie Mongrain, Marcos G Frank, Tanya Leduc

引用次数: 0

Integrating temperature into the Arabidopsis circadian system. 将温度融入拟南芥昼夜节律系统。

npj Biological Timing and Sleep Pub Date : 2025-01-01 Epub Date: 2025-10-01 DOI: 10.1038/s44323-025-00055-z

Chantal Sharples, Zoe Grace McFarlane, Maria Fernandes Pinheiro, Matthew Alan Jones

引用次数: 0

Harnessing the circadian nature of the choroid plexus and cerebrospinal fluid. 利用脉络膜丛和脑脊液的昼夜节律特性。

npj Biological Timing and Sleep Pub Date : 2025-01-01 Epub Date: 2025-05-26 DOI: 10.1038/s44323-025-00033-5

Ryann M Fame

引用次数: 0

Chronic circadian disruption alters cardiac function and glucose regulation in mice. 慢性昼夜节律紊乱会改变小鼠的心功能和葡萄糖调节。

npj Biological Timing and Sleep Pub Date : 2025-01-01 Epub Date: 2025-05-13 DOI: 10.1038/s44323-025-00032-6

Jenna E J Gearey, Melinda Wang, Michael C Antle

{"title":"Chronic circadian disruption alters cardiac function and glucose regulation in mice.","authors":"Jenna E J Gearey, Melinda Wang, Michael C Antle","doi":"10.1038/s44323-025-00032-6","DOIUrl":"https://doi.org/10.1038/s44323-025-00032-6","url":null,"abstract":"Cardiometabolic disease is a leading cause of death worldwide. One factor that may contribute to the risk, onset, and severity of symptoms is disrupted circadian rhythms. Our study uses two strains of mice to further elucidate this relationship: healthy controls, and a mouse model of insulin resistance with short freerunning periods (~ 22.75 h) and enlarged hearts, raised in either a 24-h or 22.75-h LD cycle. Through glucose and insulin tolerance tests, routine electrocardiograms from one to four months old, and histology, we reveal worse cardiometabolic health outcomes for mice gestated and housed in a mismatched LD cycle compared to those in an LD cycle that matches their endogenous rhythm. This was characterized by heightened blood glucose levels following a glucose or insulin bolus, altered electrophysiological parameters of the cardiac waveform, and increased cardiomyocyte size. Circadian disruption due to work/social schedules or circadian-related disorders in people is often confounded with other unhealthy lifestyles. The present study demonstrates that circadian disruption on its own can lead to adverse health states.","PeriodicalId":501704,"journal":{"name":"npj Biological Timing and Sleep","volume":"2 1","pages":"18"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12074981/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144083083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Challenges and opportunities for statistical power and biomarker identification arising from rhythmic variation in proteomics. 蛋白质组学中节律性变异对统计能力和生物标志物鉴定的挑战和机遇。

npj Biological Timing and Sleep Pub Date : 2025-01-01 Epub Date: 2025-01-25 DOI: 10.1038/s44323-024-00020-2

Matt Spick, Cheryl M Isherwood, Lee A Gethings, Christopher J Hughes, Matthew E Daly, Hana Hassanin, Daan R van der Veen, Debra J Skene, Jonathan D Johnston

{"title":"Challenges and opportunities for statistical power and biomarker identification arising from rhythmic variation in proteomics.","authors":"Matt Spick, Cheryl M Isherwood, Lee A Gethings, Christopher J Hughes, Matthew E Daly, Hana Hassanin, Daan R van der Veen, Debra J Skene, Jonathan D Johnston","doi":"10.1038/s44323-024-00020-2","DOIUrl":"10.1038/s44323-024-00020-2","url":null,"abstract":"Time-of-day variation in the molecular profile of biofluids and tissues is a well-described phenomenon, but-especially for proteomics-is rarely considered in terms of the challenges this presents to reproducible biomarker identification. We provide a case study analysis of human circadian and ultradian rhythmicity in proteins, including in the complement and coagulation cascades and apolipoproteins, with PLG, CFAH, ZA2G and ITIH2 demonstrated as rhythmic for the first time. We also show that rhythmicity increases the risk of Type II errors due to the reduction in statistical power from increased variance, and that controlling for rhythmic time-of-day variation improves statistical power and reduces the chances of Type II errors. We recommend that best practice in proteomics study design should account for temporal variation and that time of sampling be reported as part of study metadata. These simple steps can mitigate against both false and missed discoveries, as well as improving reproducibility.","PeriodicalId":501704,"journal":{"name":"npj Biological Timing and Sleep","volume":"2 1","pages":"3"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11762406/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143054745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shifted levels of sleep and activity during the night as mechanisms underlying ectoparasite resistance. 夜间睡眠和活动水平的变化是抵抗外寄生虫的机制。

npj Biological Timing and Sleep Pub Date : 2025-01-01 Epub Date: 2025-04-02 DOI: 10.1038/s44323-025-00031-7

Joshua B Benoit, Joy Bose, Oluwaseun M Ajayi, Ashley Webster, Karl Grieshop, David Lewis, Hailie Talbott, Michal Polak

{"title":"Shifted levels of sleep and activity during the night as mechanisms underlying ectoparasite resistance.","authors":"Joshua B Benoit, Joy Bose, Oluwaseun M Ajayi, Ashley Webster, Karl Grieshop, David Lewis, Hailie Talbott, Michal Polak","doi":"10.1038/s44323-025-00031-7","DOIUrl":"10.1038/s44323-025-00031-7","url":null,"abstract":"Parasites harm host fitness and are pervasive agents of natural selection capable of driving the evolution of host resistance traits. Previously we demonstrated evolutionary responses to artificial selection for increasing behavioral immunity to Gamasodes queenslandicus mites for Drosophila melanogaster. Here, we report transcriptional shifts in metabolic processes due to selection for mite resistance. We also show decreased starvation resistance and increased use of nutrient reserves in flies from mite-resistant lines. Resistant lines exhibited increased activity, reduced sleep, and elevated oxygen consumption during the night. Using a panel of D. melanogaster lines exhibiting variable sleep durations, we found a positive correlation between mite resistance and reduced sleep. Restraining the activity of artificially selected mite-resistant flies during exposure to parasites reduced their resistance advantage relative to control flies. The results suggest that ectoparasite resistance in this system involves increased activity during the scotophase and metabolic gene expression at the expense of starvation resistance.","PeriodicalId":501704,"journal":{"name":"npj Biological Timing and Sleep","volume":"2 1","pages":"15"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11964914/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143797546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0