The Crop Journal最新文献_第2页

Identification and transferring of a new Fusarium head blight resistance gene FhbRc2 from Roegneria ciliaris 3ScL chromosome arm into common wheat 从 Roegneria ciliaris 3ScL 染色臂中鉴定新的镰刀菌头枯病抗性基因 FhbRc2 并将其转入普通小麦中

The Crop Journal Pub Date : 2024-08-08 DOI: 10.1016/j.cj.2024.07.010

Rongrong Song, Danhua Zhang, Jingxin Yang, Yifan Cheng, Xinying Song, Weiping Zhao, Mengshuang Xia, Yao Zhang, Luyang Wei, Menghao Cheng, Wei Wang, Li Sun, Haiyan Wang, Xiu’e Wang, Jin Xiao

{"title":"Identification and transferring of a new Fusarium head blight resistance gene FhbRc2 from Roegneria ciliaris 3ScL chromosome arm into common wheat","authors":"Rongrong Song, Danhua Zhang, Jingxin Yang, Yifan Cheng, Xinying Song, Weiping Zhao, Mengshuang Xia, Yao Zhang, Luyang Wei, Menghao Cheng, Wei Wang, Li Sun, Haiyan Wang, Xiu’e Wang, Jin Xiao","doi":"10.1016/j.cj.2024.07.010","DOIUrl":"https://doi.org/10.1016/j.cj.2024.07.010","url":null,"abstract":"Fusarium head blight (FHB) threatens wheat production worldwide. Utilization of FHB resistant varieties is the most effective solution for disease control. Owing to the limited sources of FHB resistance, mining of novel resistance genes is crucial. Here, we report an FHB resistance gene from a wild wheat relative species, and developed FHB resistant germplasm containing this gene. Wheat- disomic addition line DA3S showed enhanced type II FHB resistance compared to its sister line 3S-Null without chromosome 3S, indicating that the resistance was contributed by the addition of 3S. The resistance gene on 3S was validated using F and F populations derived from the cross between DA3S and susceptible Aikang 58 (a susceptible cultivar), demonstrating that the lines with 3S had significantly enhanced FHB resistance compared to the individuals without 3S. This was the second resistance gene identified in , designated . To transfer to common wheat, we produced a double-monosomic chromosome population by crossing DA3S with the Chinese Spring nulli-tetrasomic line N3DT3B. Eight alien chromosome lines containing 3S were identified using genomic/fluorescence hybridization and 3S-specific marker analysis. Only the lines carrying the long arm of 3S conferred FHB resistance, further locating on 3SL. A compensating wheat- Robertsonian translocation line T3DS·3SL harboring is developed and provides a potential genetic resource in wheat breeding for enhanced FHB resistance.","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142186853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reduction of uncertainties in rice yield response to elevated CO2 by experiment-model integration: A case study in East China 通过试验-模型集成减少水稻产量对高浓度 CO2 响应的不确定性：华东案例研究

The Crop Journal Pub Date : 2024-08-04 DOI: 10.1016/j.cj.2024.06.012

Zihao Wang, Yu Zhang, Xueni Wang, Yanfeng Ding, Songhan Wang

引用次数: 0

One bird, multiple stones: The race to find a gene of dominant negative effect in wheat 一鸟多石寻找小麦显性负效应基因的竞赛

The Crop Journal Pub Date : 2024-08-03 DOI: 10.1016/j.cj.2024.08.001

Long Mao

引用次数: 0

Legume green manure can intensify the function of chemical nitrogen fertilizer substitution via increasing nitrogen supply and uptake of wheat 豆科绿肥可通过增加小麦的氮素供应和吸收，强化化学氮肥替代功能

The Crop Journal Pub Date : 2024-07-31 DOI: 10.1016/j.cj.2024.07.004

Jingui Wei, Zhilong Fan, Falong Hu, Shoufa Mao, Fang Yin, Qiming Wang, Qiang Chai, Wen Yin

{"title":"Legume green manure can intensify the function of chemical nitrogen fertilizer substitution via increasing nitrogen supply and uptake of wheat","authors":"Jingui Wei, Zhilong Fan, Falong Hu, Shoufa Mao, Fang Yin, Qiming Wang, Qiang Chai, Wen Yin","doi":"10.1016/j.cj.2024.07.004","DOIUrl":"https://doi.org/10.1016/j.cj.2024.07.004","url":null,"abstract":"Achieving the green development of agriculture requires the reduction of chemical nitrogen (N) fertilizer input. Previous studies have confirmed that returning green manure to the field is an effective measure to improve crop yields while substituting partial chemical N fertilizer. However, it remains unclear how to further intensify the substituting function of green manure and elucidate its underlying agronomic mechanism. In a split-plot field experiment in spring wheat, different green manures returned to the field under reduced chemical N supply was established in an oasis area since 2018, in order to investigate the effect of green manure and reduced N on grain yield, N uptake, N use efficiency (NUE), N nutrition index, soil organic matter, and soil N of wheat in 2020–2022. Our results showed that mixed sown common vetch and hairy vetch can substitute 40% of chemical N fertilizer without reducing grain yield or N accumulation. Noteworthily, mixed sown common vetch and hairy vetch under reduced N by 20% showed the highest N agronomy efficiency and recovery efficiency, which were 92.0% and 46.0% higher than fallow after wheat harvest and conventional N application rate, respectively. The increase in NUE of wheat was mainly attributed to mixed sown common vetch and hairy vetch, which increased N transportation quantity and transportation rate at pre-anthesis, enhanced N harvest index, optimized N nutrition index, and increased activities of nitrate reductase and glutamine synthetase of leaf, respectively. Meanwhile, mixed sown common vetch and hairy vetch under reduced N by 20% improved soil organic matter and N contents. Therefore, mixed sown common vetch and hairy vetch can substitute 40% of chemical N fertilizer while maintaining grain yield and N accumulation, and it combined with reduced chemical N by 20% or 40% improved NUE of wheat via enhancing N supply and uptake.","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141947842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-incorporating green manure and crop straw increases crop productivity and improves soil quality with low greenhouse-gas emissions in a crop rotation 在轮作中将绿肥和作物秸秆结合在一起，可提高作物产量，改善土壤质量，减少温室气体排放

The Crop Journal Pub Date : 2024-07-31 DOI: 10.1016/j.cj.2024.07.002

Na Zhao, Xiquan Wang, Jun Ma, Xiaohong Li, Jufeng Cao, Jie Zhou, Linmei Wu, Peiyi Zhao, Weidong Cao

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Multi-genome evolutionary study of the ABC1 gene family and identification of the pleiotropic effects of OsABC1-13 in rice development ABC1 基因家族的多基因组进化研究及 OsABC1-13 在水稻生长发育中的多效应鉴定

The Crop Journal Pub Date : 2024-07-31 DOI: 10.1016/j.cj.2024.07.005

Fuying Ma, Mingyu Liu, Peiwen Yan, Shicong He, Jian Hu, Xinwei Zhang, Fuan Niu, Jinhao Cui, Xinyu Yuan, Xiaoyun Xin, Liming Cao, Jinshui Yang, Ying Wang, Xiaojin Luo

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CRISPR/CasRx-mediated resistance to Soybean mosaic virus in soybean CRISPR/CasRx 介导的大豆对大豆花叶病毒的抗性

The Crop Journal Pub Date : 2024-07-31 DOI: 10.1016/j.cj.2024.07.007

Le Gao, Lijun Xie, Yanmin Xiao, Xinge Cheng, Ruosi Pu, Ziheng Zhang, Yu Liu, Shaopei Gao, Zilong Zhang, Haoran Qu, Haijian Zhi, Kai Li

{"title":"CRISPR/CasRx-mediated resistance to Soybean mosaic virus in soybean","authors":"Le Gao, Lijun Xie, Yanmin Xiao, Xinge Cheng, Ruosi Pu, Ziheng Zhang, Yu Liu, Shaopei Gao, Zilong Zhang, Haoran Qu, Haijian Zhi, Kai Li","doi":"10.1016/j.cj.2024.07.007","DOIUrl":"https://doi.org/10.1016/j.cj.2024.07.007","url":null,"abstract":"(SMV), an RNA virus, is the most common and destructive pathogenic virus in soybean fields. The newly developed CRISPR/Cas immune system has provided a novel strategy for improving plant resistance to viruses; hence, this study aimed to engineer SMV resistance in soybean using this system. Specifically, multiple sgRNAs were designed to target positive- and/or negative-sense strands of the SMV gene. Subsequently, the corresponding CRISPR/CasRx vectors were constructed and transformed into soybeans. After inoculation with SMV, 39.02%, 35.77%, and 18.70% of T plants were confirmed to be highly resistant (HR), resistant (R), and mildly resistant (MR) to SMV, respectively, whereas only 6.50% were identified as susceptible (S). Additionally, qRT-PCR and DAS-ELISA showed that, both at 15 and 30 d post-inoculation (dpi), SMV accumulation significantly decreased or was even undetectable in HR and R plants, followed by MR and S plants. Additionally, the expression level of the gene varied in almost all T plants with different resistance level, both at 15 and 30 dpi. Furthermore, when SMV resistance was evaluated in the T generation, the results were similar to those recorded for the T generation. These findings provide new insights into the application of the CRISPR/CasRx system for soybean improvement and offer a promising alternative strategy for breeding for resistance to biotic stress that will contribute to the development of SMV-immune soybean germplasm to accelerate progress towards greater soybean crop productivity.","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141947844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The DUF579 proteins GhIRX15s regulate cotton fiber development by interacting with proteins involved in xylan synthesis DUF579 蛋白质 GhIRX15s 通过与参与木聚糖合成的蛋白质相互作用来调节棉纤维的发育

The Crop Journal Pub Date : 2024-07-31 DOI: 10.1016/j.cj.2024.07.006

Mengyun Li, Feng Chen, Jingwen Luo, Yanan Gao, Jinglong Cai, Wei Zeng, Monika S. Doblin, Gengqing Huang, Wenliang Xu

{"title":"The DUF579 proteins GhIRX15s regulate cotton fiber development by interacting with proteins involved in xylan synthesis","authors":"Mengyun Li, Feng Chen, Jingwen Luo, Yanan Gao, Jinglong Cai, Wei Zeng, Monika S. Doblin, Gengqing Huang, Wenliang Xu","doi":"10.1016/j.cj.2024.07.006","DOIUrl":"https://doi.org/10.1016/j.cj.2024.07.006","url":null,"abstract":"Cotton provides the most abundant natural fiber for the textile industry. The mature cotton fiber largely consists of secondary cell walls with the highest proportion of cellulose and a small amount of hemicellulose and lignin. To dissect the roles of hemicellulosic polysaccharides during fiber development, four genes, ///, were functionally characterized in cotton. These genes encode DUF579 domain-containing proteins, which are homologs of AtIRX15 involved in xylan biosynthesis. The four genes were predominantly expressed during fiber secondary wall thickening, and the encoded proteins were localized to the Golgi apparatus. Each gene could restore the xylan deficient phenotype in the Arabidopsis double mutant. Silencing of s in cotton resulted in shorter mature fibers with a thinner cell wall and reduced cellulose content as compared to the wild type. Intriguingly, GhIRX15-2 and GhIRX15-4 formed homodimers and heterodimers. In addition, the GhIRX15s showed physical interaction with glycosyltransferases GhGT43C, GhGT47A and GhGT47B, which are responsible for synthesis of the xylan backbone and reducing end sequence. Moreover, the GhIRX15s can form heterocomplexes with enzymes involved in xylan modification and side chain synthesis, such as GhGUX1/2, GhGXM1/2 and GhTBL1. These findings suggest that GhIRX15s participate in fiber xylan biosynthesis and modulate fiber development via forming large multiprotein complexes.","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141947653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Utilizing auxin dwarf genes to optimize seed yield and lodging resistance in rapeseed 利用辅助素矮化基因优化油菜籽产量和抗倒伏性

The Crop Journal Pub Date : 2024-07-31 DOI: 10.1016/j.cj.2024.07.008

Hongxiang Lou, Yan Peng, Chunyun Wang, Zongkai Wang, Bowen Zhao, Ali Mahmoud El-Badri, Maria Batool, Bo Wang, Jing Wang, Zhenghua Xu, Jie Zhao, Jie Kuai, Guangsheng Zhou

{"title":"Utilizing auxin dwarf genes to optimize seed yield and lodging resistance in rapeseed","authors":"Hongxiang Lou, Yan Peng, Chunyun Wang, Zongkai Wang, Bowen Zhao, Ali Mahmoud El-Badri, Maria Batool, Bo Wang, Jing Wang, Zhenghua Xu, Jie Zhao, Jie Kuai, Guangsheng Zhou","doi":"10.1016/j.cj.2024.07.008","DOIUrl":"https://doi.org/10.1016/j.cj.2024.07.008","url":null,"abstract":"Direct-seeding rapeseed production at high plant density raises the risk of lodging. We investigated the use of dwarf genes to improve rapeseed plant architecture to balance yield and lodging. Three genotypes with different plant architectures (dwarf , semi-dwarf , and tall HS5) were evaluated under varying nitrogen rates (N1, N2, and N3: 120, 240, and 360 kg N ha) and plant densities (D1, D2, and D3: 15, 45, and 75 plants m) from 2019 to 2022. The results showed that increasing N rate positively influenced yield while decreasing lodging resistance in all genotypes. Increasing plant density (D2–D3) enhanced lodging resistance and yield in and +/, but reduced yield in HS5. Compared to the two parents, +/ exhibited moderate expressions of , , and in stems under N2D3, resulting in reduced plant height and increased compactness. Additionally, +/ had a thicker silique layer than HS5 by 14.7%, and it had a significant correlation between branch height/angle and yield. Increasing N rate led to increased lignin and pectin contents, while cellulose content decreased. Increasing plant density resulted in greater stem cellulose content and expression in and +/, but decreased in HS5. Compared to HS5, +/ exhibited higher expressions of and , along with a 51.1% increase in pectin content, leading to improved lodging resistance under N2D3. Consequently, +/ showed a 46.4% higher yield and 38.9% lodging resistance than HS5 under N2D3, while demonstrated strong lodging resistance but lower yield potential. Overall, this study underscores the potential of utilizing auxin dwarf genes to optimize the trade-off between yield and lodging resistance in rapeseed and the possibility of maximizing yield potential by optimizing the plant architecture of +/ through nitrogen reduction and dense planting.","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"67 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141947652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transcriptional landscape of the developmental switch from regular pollen maturation towards microspore-derived plant regeneration in barley 大麦从常规花粉成熟到小孢子植物再生的发育转换过程中的转录图谱

The Crop Journal Pub Date : 2024-07-30 DOI: 10.1016/j.cj.2024.07.003

Anna Nowicka, Martin Kovacik, Anna Maksylewicz, Przemysław Kopeć, Ewa Dubas, Monika Krzewska, Agnieszka Springer, Robert E. Hoffie, Diaaeldin S. Daghma, Zbyněk Milec, Ales Pecinka, Jochen Kumlehn, Iwona Żur

{"title":"The transcriptional landscape of the developmental switch from regular pollen maturation towards microspore-derived plant regeneration in barley","authors":"Anna Nowicka, Martin Kovacik, Anna Maksylewicz, Przemysław Kopeć, Ewa Dubas, Monika Krzewska, Agnieszka Springer, Robert E. Hoffie, Diaaeldin S. Daghma, Zbyněk Milec, Ales Pecinka, Jochen Kumlehn, Iwona Żur","doi":"10.1016/j.cj.2024.07.003","DOIUrl":"https://doi.org/10.1016/j.cj.2024.07.003","url":null,"abstract":"Plant formation from -cultivated microspores involves a complex network of internal and environmental factors. Haploids/doubled haploids (DHs) derived from -cultured microspores are widely used in plant breeding and genetic engineering. However, the mechanism underlying the developmental switch from regular pollen maturation towards microspore-derived plant regeneration remains poorly defined. Here, RNA-sequencing was employed to elucidate the transcriptional landscapes of four early stages of microspore embryogenesis (ME) in barley cultivars Golden Promise and Igri, which exhibit contrasting responsiveness to microspore-derived plant formation. Our experiments revealed fundamental regulatory networks, specific groups of genes, and transcription factor (TF) families potentially regulating the developmental switch. We identified a set of candidate genes crucial for genotype-dependent responsiveness/recalcitrance to ME. Our high-resolution temporal transcriptome atlas provides an important resource for future functional studies on the genetic control of microspore developmental transition.","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"91 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141947845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0