The Crop Journal最新文献_第10页

The ABA synthesis enzyme allele OsNCED2T promotes dryland adaptation in upland rice ABA 合成酶等位基因 OsNCED2T 促进高原水稻的旱地适应性

The Crop Journal Pub Date : 2024-01-06 DOI: 10.1016/j.cj.2023.12.001

Liyu Huang, Yachong Bao, Shiwen Qin, Min Ning, Qinyan Li, Qingmao Li, Shilai Zhang, Guangfu Huang, Jing Zhang, Wensheng Wang, Binying Fu, Fengyi Hu

{"title":"The ABA synthesis enzyme allele OsNCED2T promotes dryland adaptation in upland rice","authors":"Liyu Huang, Yachong Bao, Shiwen Qin, Min Ning, Qinyan Li, Qingmao Li, Shilai Zhang, Guangfu Huang, Jing Zhang, Wensheng Wang, Binying Fu, Fengyi Hu","doi":"10.1016/j.cj.2023.12.001","DOIUrl":"https://doi.org/10.1016/j.cj.2023.12.001","url":null,"abstract":"Upland rice shows dryland adaptation in the form of a deeper and denser root system and greater drought resistance than its counterpart, irrigated rice. Our previous study revealed a difference in the frequency of the OsNCED2 gene between upland and irrigated populations. A nonsynonymous mutation (C to T, from irrigated to upland rice) may have led to functional variation fixed by artificial selection, but the exact biological function in dryland adaptation is unclear. In this study, transgenic and association analysis indicated that the domesticated fixed mutation caused functional variation in OsNCED2, increasing ABA levels, root development, and drought tolerance in upland rice under dryland conditions. OsNCED2-overexpressing rice showed increased reactive oxygen species-scavenging abilities and transcription levels of many genes functioning in stress response and development that may regulate root development and drought tolerance. OsNCED2T-NILs showed a denser root system and drought resistance, promoting the yield of rice under dryland conditions. OsNCED2T may confer dryland adaptation in upland rice and may find use in breeding dryland-adapted, water-saving rice.","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139375014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sugarcane transcription factor ScWRKY4 negatively regulates resistance to pathogen infection through the JA signaling pathway 甘蔗转录因子 ScWRKY4 通过 JA 信号途径负向调节对病原体感染的抵抗力

The Crop Journal Pub Date : 2024-01-06 DOI: 10.1016/j.cj.2023.12.002

Dongjiao Wang, Wei Wang, Shoujian Zang, Liqian Qin, Yanlan Liang, Peixia Lin, Yachun Su, Youxiong Que

{"title":"Sugarcane transcription factor ScWRKY4 negatively regulates resistance to pathogen infection through the JA signaling pathway","authors":"Dongjiao Wang, Wei Wang, Shoujian Zang, Liqian Qin, Yanlan Liang, Peixia Lin, Yachun Su, Youxiong Que","doi":"10.1016/j.cj.2023.12.002","DOIUrl":"https://doi.org/10.1016/j.cj.2023.12.002","url":null,"abstract":"WRKY transcription factors, transcriptional regulators unique to plants, play an important role in defense response to pathogen infection. However, the resistance mechanisms of WRKY genes in sugarcane remain unclear. In the present study, gene ontology (GO) enrichment analysis revealed that WRKY gene family in sugarcane was extensively involved in the response to biotic stress and in defense response. We identified gene ScWRKY4, a class IIc member of the WRKY gene family, in sugarcane cultivar ROC22. This gene was induced by salicylic acid (SA) and methyl jasmonate (MeJA) stress. Interestingly, expression of ScWRKY4 was down-regulated in smut-resistant sugarcane cultivars but up-regulated in smut-susceptible sugarcane cultivars infected with Sporisorium scitamineum. Moreover, stable overexpression of the ScWRKY4 gene in Nicotiana benthamiana enhanced susceptibility to Fusarium solani var. coeruleum and caused down-regulated expression of immune marker-related genes. Transcriptome analysis indicated suppressed expression of most JAZ genes in the signal transduction pathway. ScWRKY4 interacted with ScJAZ13 to repress its expression. We thus hypothesized that the ScWRKY4 gene was involved in the regulatory network of plant disease resistance, most likely through the JA signaling pathway. The present study depicting the molecular involvement of ScWRKY4 in sugarcane disease resistance lays a foundation for future investigation.","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139374849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of abiotic stresses on plant C:N:P homeostasis and their mitigation by silicon 非生物胁迫对植物 C:N:P 平衡的影响及其对硅的缓解作用

The Crop Journal Pub Date : 2024-01-01 DOI: 10.1016/j.cj.2023.11.012

M. Costa, Renato de Mello Prado, L. F. Palaretti, J. P. de Souza Júnior

引用次数: 0

Elite, transformable haploid inducers in maize 玉米中可转化的精英单倍体诱导体

The Crop Journal Pub Date : 2023-12-21 DOI: 10.1016/j.cj.2023.10.016

Brent Delzer, Dawei Liang, David Szwerdszarf, Isadora Rodriguez, Gonzalo Mardones, Sivamani Elumalai, Francine Johnson, Samson Nalapalli, Rachel Egger, Erin Burch, Kerry Meier, Juan Wei, Xiujuan Zhang, Huaping Gui, Huaibing Jin, Huan Guo, Kun Yu, Yubo Liu, Becky Breitinger, Ana Poets, Timothy Kelliher

{"title":"Elite, transformable haploid inducers in maize","authors":"Brent Delzer, Dawei Liang, David Szwerdszarf, Isadora Rodriguez, Gonzalo Mardones, Sivamani Elumalai, Francine Johnson, Samson Nalapalli, Rachel Egger, Erin Burch, Kerry Meier, Juan Wei, Xiujuan Zhang, Huaping Gui, Huaibing Jin, Huan Guo, Kun Yu, Yubo Liu, Becky Breitinger, Ana Poets, Timothy Kelliher","doi":"10.1016/j.cj.2023.10.016","DOIUrl":"https://doi.org/10.1016/j.cj.2023.10.016","url":null,"abstract":"The introduction of alleles into commercial crop breeding pipelines is both time consuming and costly. Two technologies that are disrupting traditional breeding processes are doubled haploid (DH) breeding and genome editing (GE). Recently, these techniques were combined into a GE trait delivery system called HI-Edit (Haploid Inducer – Edit) [1]. In HI-Edit, the pollen of a haploid inducer line is reprogrammed to deliver GE traits to any variety, obviating recurrent selection. For HI-Edit to operate at scale, an efficient transformable HI line is needed, but most maize varieties are recalcitrant to transformation, and haploid inducers are especially difficult to transform given their aberrant reproductive behaviors. Leveraging marker assisted selection and a three-tiered testing scheme, we report the development of new Iodent and Stiff Stalk maize germplasm that are transformable, have high haploid induction rates, and exhibit a robust, genetically-dominant anthocyanin native trait that may be used for rapid haploid identification. We show that transformation of these elite “HI-Edit” lines is enhanced using the BABYBOOM and WUSCHEL morphogenetic factors. Finally, we evaluate the HI-Edit performance of one of the lines against both Stiff Stalk and non-Stiff Stalk testers. The strategy and results of this study should facilitate the development of commercially scalable HI-Edit systems in diverse crops.","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139026904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The OsBSK1-2-MAPK module regulates blast resistance in rice OsBSK1-2-MAPK 模块调控水稻的抗稻瘟病能力

The Crop Journal Pub Date : 2023-12-21 DOI: 10.1016/j.cj.2023.11.009

Shengping Li, Xinquan Xiang, Zhijuan Diao, Na Xia, Ling Lu, Jing Zhang, Zhiwei Chen, Dingzhong Tang

{"title":"The OsBSK1-2-MAPK module regulates blast resistance in rice","authors":"Shengping Li, Xinquan Xiang, Zhijuan Diao, Na Xia, Ling Lu, Jing Zhang, Zhiwei Chen, Dingzhong Tang","doi":"10.1016/j.cj.2023.11.009","DOIUrl":"https://doi.org/10.1016/j.cj.2023.11.009","url":null,"abstract":"Receptor-like cytoplasmic kinase OsBSK1-2 was reported to play an important role in regulation of response to rice blast, but the signaling pathway remained unknown. In this study, we identified OsMAPKKK18 and previously uncharacterized MAPKKKs OsMAPKKK16 and OsMAPKKK19 that interact with OsBSK1-2. Expression of all three MAPKKKs was induced by Magnaporthe oryzae infection, and all three induced cell death when transiently expressed in Nicotiana benthamiana leaves. Knockout of OsMAPKKK16 and OsMAPKKK18 compromised blast resistance and overexpression of OsMAPKKK19 increased blast resistance, indicating that all three MAPKKKs are involved in regulation of rice blast response. Furthermore, both OsMAPKKK16 and OsMAPKKK19 interacted with and phosphorylated OsMKK4 and OsMKK5, and chitin-induced MAPK activation was suppressed in osmapkkk16 and osbsk1-2 mutants. OsMAPKKK18 was earlier reported to interact with and phosphorylate OsMKK4 and affect chitin-induced MAPK activation, suggesting that OsBSK1-2 is involved in regulation of immunity through multiple MAPK signaling pathways. Unlike BSK1 in Arabidopsis, OsBSK1-2 was not involved in response to avirulent M. oryzae strains. Taken together, our results revealed important roles of OsMAPKKK16/18/19 and a OsBSK1-2-OsMAPKKK16/18/19-OsMKK4/5 module in regulating response to rice blast.","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"81 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139026902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Searching for plant NLR immune receptors conferring resistance to potyviruses 寻找能抵抗壶形病毒的植物 NLR 免疫受体

The Crop Journal Pub Date : 2023-12-20 DOI: 10.1016/j.cj.2023.11.010

Xin Hong, Shufen Li, Xiaofei Cheng, Haijian Zhi, Jinlong Yin, Kai Xu

引用次数: 0

Wetting alternating with partial drying during grain filling increases lysine biosynthesis in inferior rice grain 谷粒灌浆期湿润与部分干燥交替进行可增加劣质稻谷中赖氨酸的生物合成

The Crop Journal Pub Date : 2023-12-19 DOI: 10.1016/j.cj.2023.11.008

Yi Jiang, Wenli Tao, Weiyang Zhang, Zhiqin Wang, Jianchang Yang

{"title":"Wetting alternating with partial drying during grain filling increases lysine biosynthesis in inferior rice grain","authors":"Yi Jiang, Wenli Tao, Weiyang Zhang, Zhiqin Wang, Jianchang Yang","doi":"10.1016/j.cj.2023.11.008","DOIUrl":"https://doi.org/10.1016/j.cj.2023.11.008","url":null,"abstract":"Lysine content is a criterion of the nutritional quality of rice. Understanding the process of lysine biosynthesis in early-flowering superior grain (SG) and late-flowering inferior grain (IG) of rice would advance breeding and cultivation to improve nutritional quality. However, little information is available on differences in lysine anabolism between SG and IG and the underlying mechanism, and whether and how irrigation regimes affect lysine anabolism in these grains. A japonica rice cultivar was grown in the field and two irrigation regimes, continuous flooding (CF) and wetting alternating with partial drying (WAPD), were imposed from heading to the mature stage. Lysine content and activities of key enzymes of lysine biosynthesis, and levels of brassinosteroids (BRs) were lower in the IG than in the SG at the early grain-filling stage but higher at middle and late grain-filling stages. WAPD increased activities of these key enzymes, BR levels, and contents of lysine and total amino acids in IG, but not SG relative to CF. Application of 2,4-epibrassinolide to rice panicles in CF during early grain filling reproduced the effects of WAPD, but neither treatment altered the activities of enzymes responsible for lysine catabolism in either SG or IG. WAPD and elevated BR levels during grain filling increased lysine biosynthesis in IG. Improvement in lysine biosynthesis in rice should focus on IG.","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138741574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Camellia sinensis CsMYB4a participates in regulation of stamen growth by interaction with auxin signaling transduction repressor CsAUX/IAA4 茶树 CsMYB4a 通过与植物生长素信号转导抑制因子 CsAUX/IAA4 相互作用参与调节雄蕊生长

The Crop Journal Pub Date : 2023-12-17 DOI: 10.1016/j.cj.2023.11.006

Guoliang Ma, Mingzhuo Li, Yingling Wu, Changjuan Jiang, Yifan Chen, Dawei Xing, Yue Zhao, Yajun Liu, Xiaolan Jiang, Tao Xia, Liping Gao

{"title":"Camellia sinensis CsMYB4a participates in regulation of stamen growth by interaction with auxin signaling transduction repressor CsAUX/IAA4","authors":"Guoliang Ma, Mingzhuo Li, Yingling Wu, Changjuan Jiang, Yifan Chen, Dawei Xing, Yue Zhao, Yajun Liu, Xiaolan Jiang, Tao Xia, Liping Gao","doi":"10.1016/j.cj.2023.11.006","DOIUrl":"https://doi.org/10.1016/j.cj.2023.11.006","url":null,"abstract":"Subgroup 4 (Sg4) members of the R2R3-MYB are generally known as negative regulators of the phenylpropanoid pathway in plants. Our previous research showed that a R2R3-MYB Sg4 member from Camellia sinensis (CsMYB4a) inhibits expression of some genes in the phenylpropanoid pathway, but its physiological function in the tea plant remained unknown. Here, CsMYB4a was found to be highly expressed in anther and filaments, and participated in regulating filament growth. Transcriptome analysis and exogenous auxin treatment showed that the target of CsMYB4a might be the auxin signal pathway. Auxin/indole-3-acetic acid 4 (AUX/IAA4), a repressor in auxin signal transduction, was detected from a yeast two-hybrid screen using CsMYB4a as bait. Gene silencing assays showed that both CsIAA4 and CsMYB4a regulate filament growth. Tobacco plants overexpressing CsIAA4 were insensitive to exogenous α-NAA, consistent with overexpression of CsMYB4a. Protein-protein interaction experiments revealed that CsMYB4a interacts with N-terminal of CsIAA4 to prevent CsIAA4 degradation. Knock out of the endogenous NtIAA4 gene, a CsIAA4 homolog, in tobacco alleviated filament growth inhibition and α-NAA insensitivity in plants overexpressing CsMYB4a. All results strongly suggest that CsMYB4a works synergistically with CsIAA4 and participates in regulation of the auxin pathway in stamen.","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"197 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138687505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The wheat sucrose synthase gene TaSus1 is a major determinant of grain number per spike 小麦蔗糖合成酶基因 TaSus1 是每穗粒数的主要决定因素

The Crop Journal Pub Date : 2023-12-17 DOI: 10.1016/j.cj.2023.11.007

Liping Shen, Lili Zhang, Changbin Yin, Xiaowan Xu, Yangyang Liu, Kuocheng Shen, He Wu, Zhiwen Sun, Ke Wang, Zhonghu He, Xueyong Zhang, Chenyang Hao, Jian Hou, Aoyue Bi, Xuebo Zhao, Daxing Xu, Botao Ye, Xuchang Yu, Ziying Wang, Danni Liu, Zifeng Guo

{"title":"The wheat sucrose synthase gene TaSus1 is a major determinant of grain number per spike","authors":"Liping Shen, Lili Zhang, Changbin Yin, Xiaowan Xu, Yangyang Liu, Kuocheng Shen, He Wu, Zhiwen Sun, Ke Wang, Zhonghu He, Xueyong Zhang, Chenyang Hao, Jian Hou, Aoyue Bi, Xuebo Zhao, Daxing Xu, Botao Ye, Xuchang Yu, Ziying Wang, Danni Liu, Zifeng Guo","doi":"10.1016/j.cj.2023.11.007","DOIUrl":"https://doi.org/10.1016/j.cj.2023.11.007","url":null,"abstract":"Some haplotypes of the sucrose synthase gene TaSus1 are associated with thousand-grain weight (TGW) in wheat (Triticum aestivum L.). However, no mutations have been identified within the gene to test this association. The effects of TaSus1 on grain number per spike (GNS) also are largely unknown. Our previous genome-wide association study identified TaSus-A1 as a candidate gene controlling fertile spikelet number per spike (FSN). In the present study, we generated two independent mutants for the three TaSus1 homoeologs by CRISPR/Cas9-mediated genome editing. The triple mutants displayed lower FSN, GNS, grain number per spikelet (GNST), and TGW than wild-type plants. In 306 hexaploid wheat accessions, two single-nucleotide polymorphisms in TaSus-A1 contributed differently to GNS. Introgression of the two alleles into a wheat genetic background confirmed their effects. The alleles differed in geographical distribution among the accessions.","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138687508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Global characterization of OsPIP aquaporins reveals that the H2O2 transporter OsPIP2;6 increases resistance to rice blast OsPIP 水蒸发蛋白的全球特性分析表明，H2O2 转运体 OsPIP2;6 可增强对稻瘟病的抗性

The Crop Journal Pub Date : 2023-12-14 DOI: 10.1016/j.cj.2023.11.004

Gousi Li, Jingluan Han, Chen Yi, Hao Luo, Yuzhu Wang, Fengpin Wang, Xiaoyu Wang, Letian Chen, Yaling Zhang

{"title":"Global characterization of OsPIP aquaporins reveals that the H2O2 transporter OsPIP2;6 increases resistance to rice blast","authors":"Gousi Li, Jingluan Han, Chen Yi, Hao Luo, Yuzhu Wang, Fengpin Wang, Xiaoyu Wang, Letian Chen, Yaling Zhang","doi":"10.1016/j.cj.2023.11.004","DOIUrl":"https://doi.org/10.1016/j.cj.2023.11.004","url":null,"abstract":"Plasma membrane intrinsic proteins (PIPs) are conserved plant aquaporins that transport small molecules across the plasma membrane to trigger instant stress responses and maintain cellular homeostasis under biotic and abiotic stress. To elucidate their roles in plant immunity to pathogen attack, we characterized the expression patterns, subcellular localizations, and H2O2-transport ability of 11 OsPIPs in rice (Oryza sativa), and identified OsPIP2;6 as necessary for rice disease resistance. OsPIP2;6 resides on the plasma membrane and facilitates cytoplasmic import of the immune signaling molecule H2O2. Knockout of OsPIP2;6 increases rice susceptibility to Magnaporthe oryzae, indicating a positive function in plant immunity. OsPIP2;6 interacts with OsPIP2;2, which has been reported to increase rice resistance to pathogens via H2O2 transport. Our findings suggest that OsPIP2;6 cooperates with OsPIP2;2 as a defense signal transporter complex during plant–pathogen interaction.","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138687413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0