The wheat sucrose synthase gene TaSus1 is a major determinant of grain number per spike

The Crop Journal Pub Date : 2023-12-17 DOI:10.1016/j.cj.2023.11.007

Liping Shen, Lili Zhang, Changbin Yin, Xiaowan Xu, Yangyang Liu, Kuocheng Shen, He Wu, Zhiwen Sun, Ke Wang, Zhonghu He, Xueyong Zhang, Chenyang Hao, Jian Hou, Aoyue Bi, Xuebo Zhao, Daxing Xu, Botao Ye, Xuchang Yu, Ziying Wang, Danni Liu, Zifeng Guo

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Abstract

Some haplotypes of the sucrose synthase gene TaSus1 are associated with thousand-grain weight (TGW) in wheat (Triticum aestivum L.). However, no mutations have been identified within the gene to test this association. The effects of TaSus1 on grain number per spike (GNS) also are largely unknown. Our previous genome-wide association study identified TaSus-A1 as a candidate gene controlling fertile spikelet number per spike (FSN). In the present study, we generated two independent mutants for the three TaSus1 homoeologs by CRISPR/Cas9-mediated genome editing. The triple mutants displayed lower FSN, GNS, grain number per spikelet (GNST), and TGW than wild-type plants. In 306 hexaploid wheat accessions, two single-nucleotide polymorphisms in TaSus-A1 contributed differently to GNS. Introgression of the two alleles into a wheat genetic background confirmed their effects. The alleles differed in geographical distribution among the accessions.

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小麦蔗糖合成酶基因 TaSus1 是每穗粒数的主要决定因素

蔗糖合成酶基因 TaSus1 的某些单倍型与小麦（Triticum aestivum L.）的千粒重（TGW）有关。然而，在该基因中尚未发现突变来检验这种关联。TaSus1 对每穗粒数（GNS）的影响在很大程度上也是未知的。我们之前的全基因组关联研究发现，TaSus-A1 是控制每穗可育小穗数（FSN）的候选基因。在本研究中，我们通过 CRISPR/Cas9 介导的基因组编辑产生了两个独立的 TaSus1 同源物突变体。三重突变体的 FSN、GNS、每穗粒数（GNST）和 TGW 均低于野生型植株。在 306 个六倍体小麦品种中，TaSus-A1 的两个单核苷酸多态性对 GNS 的影响不同。将这两个等位基因引入小麦遗传背景证实了它们的影响。这两个等位基因在小麦品种中的地理分布不同。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The Crop Journal

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