Anna Nowicka, Martin Kovacik, Anna Maksylewicz, Przemysław Kopeć, Ewa Dubas, Monika Krzewska, Agnieszka Springer, Robert E. Hoffie, Diaaeldin S. Daghma, Zbyněk Milec, Ales Pecinka, Jochen Kumlehn, Iwona Żur
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引用次数: 0
Abstract
Plant formation from -cultivated microspores involves a complex network of internal and environmental factors. Haploids/doubled haploids (DHs) derived from -cultured microspores are widely used in plant breeding and genetic engineering. However, the mechanism underlying the developmental switch from regular pollen maturation towards microspore-derived plant regeneration remains poorly defined. Here, RNA-sequencing was employed to elucidate the transcriptional landscapes of four early stages of microspore embryogenesis (ME) in barley cultivars Golden Promise and Igri, which exhibit contrasting responsiveness to microspore-derived plant formation. Our experiments revealed fundamental regulatory networks, specific groups of genes, and transcription factor (TF) families potentially regulating the developmental switch. We identified a set of candidate genes crucial for genotype-dependent responsiveness/recalcitrance to ME. Our high-resolution temporal transcriptome atlas provides an important resource for future functional studies on the genetic control of microspore developmental transition.
由培养的小孢子形成植物涉及复杂的内部和环境因素网络。由培养小孢子产生的单倍体/双倍单倍体（DHs）被广泛用于植物育种和基因工程。然而，从常规花粉成熟到小孢子衍生植物再生的发育转换机制仍未明确。在这里，我们利用 RNA 测序阐明了大麦栽培品种 Golden Promise 和 Igri 小孢子胚胎发生（ME）四个早期阶段的转录景观，这两个品种对小孢子衍生植物形成的反应性截然不同。我们的实验揭示了可能调控发育开关的基本调控网络、特定基因组和转录因子（TF）家族。我们确定了一组候选基因，它们对基因型依赖的 ME 反应性/再反应性至关重要。我们的高分辨率时间转录组图谱为未来小孢子发育转换遗传调控的功能研究提供了重要资源。
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