Luminescence最新文献_第5页

Spectrofluorimetric Determination of 7-Aminoclonazepam, a Major Clonazepam Metabolite, in Human Urine: A Factorial Design of Experiment Approach to Optimize Method Development 光谱荧光测定法测定人尿液中的主要氯硝西泮代谢物 7-氨基氯硝西泮：采用因子实验设计法优化方法开发。

IF 3.2 4区化学

Luminescence Pub Date : 2024-12-12 DOI: 10.1002/bio.70049

Maram H. Abduljabbar, Yusuf S. Althobaiti, Reem M. Alnemari, Majed A. Algarni, Ahmed K. Bamaga, Adnan Alharbi, Ahmed Serag, Atiah H. Almalki

{"title":"Spectrofluorimetric Determination of 7-Aminoclonazepam, a Major Clonazepam Metabolite, in Human Urine: A Factorial Design of Experiment Approach to Optimize Method Development","authors":"Maram H. Abduljabbar, Yusuf S. Althobaiti, Reem M. Alnemari, Majed A. Algarni, Ahmed K. Bamaga, Adnan Alharbi, Ahmed Serag, Atiah H. Almalki","doi":"10.1002/bio.70049","DOIUrl":"10.1002/bio.70049","url":null,"abstract":"<div>\u0000 \u0000 Clonazepam, a high-potency benzodiazepine widely prescribed for seizure and panic disorders, carries a risk of abuse and dependency. This study developed a sensitive and selective spectrofluorimetric method for determining 7-aminoclonazepam, the major metabolite of clonazepam, in human urine. A 26-2 factorial design was employed to screen the optimal conditions for derivatization with NBD-Cl as the fluorescent label, considering factors such as pH, reagent volumes, temperature, and reaction time. A significant model was attained (p < 0.0001) revealing alkaline pH (9), elevated temperature (80°C), and high reagent concentrations as crucial for maximizing fluorescence intensity. The method demonstrated excellent linearity from 10 to 500 ng/mL (R2 = 0.9997), with limits of detection and quantitation of 3.3 and 10 ng/mL, respectively. Intra- and inter-day precision (% RSD) were less than 4%, and recoveries ranged from 97.59% to 106.12%. The method also showed no significant interference from endogenous compounds, pharmaceutical excipients, or the parent drug. Applicability of the method was validated in human subjects receiving clonazepam therapy; 7-aminoclonazepam was first detected after 12 h, peaked at 24 h (54.61 ± 9.870 ng/mL), and remained detectable up to 72 h post-dose, offering a simple, cost-effective spectrofluorimetric method for monitoring clonazepam metabolism in clinical and forensic settings.\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"39 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142819945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Facile Approach to Develop Peptide-Stabilized CdS/CdSe Quantum Dots for Cellular Imaging 开发用于细胞成像的肽稳定CdS/CdSe量子点的简易方法。

IF 3.2 4区化学

Luminescence Pub Date : 2024-12-11 DOI: 10.1002/bio.70048

Rohit Kumar Singh, Uday Suryakanta, Bijayananda Panigrahi, Sourav Mishra, Swatilekha Das, Sushil Ranjan Bhatta, Sunil K. Ghosh, Dindyal Mandal

{"title":"Facile Approach to Develop Peptide-Stabilized CdS/CdSe Quantum Dots for Cellular Imaging","authors":"Rohit Kumar Singh, Uday Suryakanta, Bijayananda Panigrahi, Sourav Mishra, Swatilekha Das, Sushil Ranjan Bhatta, Sunil K. Ghosh, Dindyal Mandal","doi":"10.1002/bio.70048","DOIUrl":"10.1002/bio.70048","url":null,"abstract":"<div>\u0000 \u0000 This study presents a mild, one-pot synthetic approach for the synthesis of multicolor, water soluble, photo luminescent CdS and CdSe quantum dots (QDs). To achieve this goal, cyclic peptides containing cysteine residues are rationally designed and synthesized. Among the peptides tested, those containing two cysteine residues exhibit superior stabilizing properties, ensuring the solubility and long-term stability of the QDs in aqueous solutions for several months. The newly synthesized QDs exhibit unique excitation-dependent multicolor photoluminescence with a quantum yield of 20.55% and 45.50% for CdS and CdSe, respectively, providing versatility for imaging applications. Cellular uptake studies using HCT 116 cells reveal effective internalization of the QDs into both the cytoplasm and nucleus, highlighting their potential applications in bioimaging and drug delivery. This green synthesis approach underscores the crucial role of peptide chemistry in nanoparticle stabilization, paving the way for the development of functional nanomaterials tailored for specific uses in bioimaging and nanomedicine.\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"39 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142808464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Eco-Friendly and Sensitive Microwave-Aided Spectrofluorophotometric Characterization of Mirabegron-Loaded Nanocarriers by Amalgamation of Design of Experiments and White Analytical Chemistry 利用实验设计与白色分析化学相结合的微波辅助荧光光谱法表征负载miraberon的纳米载体。

IF 3.2 4区化学

Luminescence Pub Date : 2024-12-11 DOI: 10.1002/bio.70046

Pintu Prajapati, Krishna Patel, Pranav Shah, Vilas Surana, Anzarul Haque, Mohd Abul Kalam, Shailesh Shah

{"title":"An Eco-Friendly and Sensitive Microwave-Aided Spectrofluorophotometric Characterization of Mirabegron-Loaded Nanocarriers by Amalgamation of Design of Experiments and White Analytical Chemistry","authors":"Pintu Prajapati, Krishna Patel, Pranav Shah, Vilas Surana, Anzarul Haque, Mohd Abul Kalam, Shailesh Shah","doi":"10.1002/bio.70046","DOIUrl":"10.1002/bio.70046","url":null,"abstract":"<div>\u0000 \u0000 Mirabegron, a drug for treatment of overactive bladder, has low water solubility and bioavailability. Researchers used quality by design to develop and characterize second-generation lipid nanocarriers for mirabegron, aiming to provide spatially and temporally sustained drug release. Numerous analytical methodologies for assessing mirabegron in pharmaceutical and biological fluids use chemical solvents in the mobile phase, potentially impacting aquatic ecosystems and the environment. The issue was addressed with a microwave-assisted spectrofluorimetric technique, environmentally benign solvents, and a fluorescent probe known as 4-chloro-7-nitrobenzofuran. Researchers used screening design and response-surface methodology to minimize organic waste. The suggested technique satisfied ICH Q2 (R2) and M10 validation standards. This approach described the in vivo pharmacokinetics and in vitro drug release of mirabegron-loaded lipid nano-carriers. We anticipated the chemical reaction pathways that derivatized mirabegron with 4-chloro-7-nitrobenzofuran by the analysis of mass spectra. We assessed the proposed method's environmental effect and sustainability compared to current methods using the RGB model, an analytical greenness calculator, and green analytical process index software. The proposed mirabegron estimate method was user-friendly for analysts, cost-effective, sensitive, robust, and environmentally sustainable, adhering to sustainable analytical practices and reducing ecological consequences.\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"39 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142814712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

pH-Dependent Fluorescence Quenching of Rhodamine 6G by Graphene Oxide: A Comprehensive Spectroscopic Study 氧化石墨烯对罗丹明6G的ph依赖性荧光猝灭：一个全面的光谱研究。

IF 3.2 4区化学

Luminescence Pub Date : 2024-12-11 DOI: 10.1002/bio.70055

Jiarui Si, Yikang Liu, Yuanfei Jiang, Yutong Chen, Anmin Chen, Mingxing Jin

{"title":"pH-Dependent Fluorescence Quenching of Rhodamine 6G by Graphene Oxide: A Comprehensive Spectroscopic Study","authors":"Jiarui Si, Yikang Liu, Yuanfei Jiang, Yutong Chen, Anmin Chen, Mingxing Jin","doi":"10.1002/bio.70055","DOIUrl":"10.1002/bio.70055","url":null,"abstract":"<div>\u0000 \u0000 The fluorescence quenching behavior of rhodamine 6G (R6G) by graphene oxide (GO) under varying pH conditions was investigated. Utilizing steady-state fluorescence spectroscopy, single-photon counting, and ultrafast time-resolved absorption spectroscopy, we explored the quenching efficiency at pH values of 3, 7, and 11. Our findings reveal that GO effectively quenches R6G fluorescence across all tested pH levels, with the most significant quenching observed at pH 7. This quenching efficiency is attributed to optimal electrostatic interactions and efficient charge transfer between GO and R6G at neutral pH. At pH 3, the quenching efficiency is moderately reduced due to partial protonation of GO, which weakens electrostatic interactions but maintains hydrogen bonding. At pH 11, the quenching efficiency is lowest, likely due to increased electrostatic repulsion and reduced charge transfer resulting from deprotonation of GO. Ultrafast time-resolved absorption spectroscopy further confirmed the dynamic nature of the quenching process, showing distinct differences in relaxation kinetics across the pH spectrum. This study highlights the critical role of pH in modulating the quenching mechanisms of GO and R6G, providing valuable insights for the design of pH-sensitive fluorescence sensing systems.\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"39 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142814713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reaction-Based Fluorescence Assays for the Determination of Food Trimethylamine Oxide 反应基荧光法测定食品中氧化三甲胺。

IF 3.2 4区化学

Luminescence Pub Date : 2024-12-11 DOI: 10.1002/bio.70056

Danfeng Peng, Xinxin Huang, Qiling Wang, Liying Huang, Fang Liu, Xiaotong Xia, Fangle Liu, Chun Cai, Tao Deng

{"title":"Reaction-Based Fluorescence Assays for the Determination of Food Trimethylamine Oxide","authors":"Danfeng Peng, Xinxin Huang, Qiling Wang, Liying Huang, Fang Liu, Xiaotong Xia, Fangle Liu, Chun Cai, Tao Deng","doi":"10.1002/bio.70056","DOIUrl":"10.1002/bio.70056","url":null,"abstract":"<div>\u0000 \u0000 Trimethylamine oxide (TMAO), a microbial metabolite commonly found in foods, has been attracting increasing attention as it is associated with the risk of several diseases. Simple and accurate analytical methods are crucial for TMAO study. In the present study, we proposed a chemical reaction-based fluorescence assay for TMAO detection using synthetic small molecular probes. After systematic screening and optimization, the sensitive and selective quantification of TMAO has been achieved based on a fluorescence probe P6 (3-iodopropanyl group modified resorufin). Excellent linearity (R2 = 0.997) was found between 6.25 and 50 μM, and the limit of detection (LOD) was 0.20 μM. Using this method, TMAO levels in several marine fishes and shellfishes have been successfully analyzed. The probe-based assay offers a simple and useful way for TMAO determination. The design is inspired by the unique oxidation reaction between TMAO and halogen, which opens a new perspective in the development of more advanced analytical assays for TMAO in the future.\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"39 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142814714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Benzothiazole–Benzonitrile-Based Fluorescent Chromophore: Synthesis, DFT, Antimicrobial Properties 新型苯并噻唑-苯并腈基荧光发色团：合成、DFT、抗菌性能。

IF 3.2 4区化学

Luminescence Pub Date : 2024-12-09 DOI: 10.1002/bio.70038

Samar Y. Al Nami, Aisha Hossan

{"title":"Novel Benzothiazole–Benzonitrile-Based Fluorescent Chromophore: Synthesis, DFT, Antimicrobial Properties","authors":"Samar Y. Al Nami, Aisha Hossan","doi":"10.1002/bio.70038","DOIUrl":"10.1002/bio.70038","url":null,"abstract":"<div>\u0000 \u0000 The synthetic strategy for the benzothiazole–benzonitrile chromophore 6 involved three sequences of reactions. It was initiated by condensation of 2-amino-4-nitrothiophenol (2) with 4-(piperidin-1-yl)benzaldehyde (1) to produce the corresponding 5-nitrobenzothiazole compound 3 followed by reduction of compound 3 into the 5-aminobenzothiazole compound 4 and then ended by condensation of 5-aminobenzothiazole compound 4 with 4-formylbenzonitrile (5). The chromophore's absorption and emission spectra have been measured in EtOH and presented good Stokes' shift (<math>\u0000 <semantics>\u0000 <mrow>\u0000 <mo>∆</mo>\u0000 <mover>\u0000 <mi>ν</mi>\u0000 <mo>¯</mo>\u0000 </mover>\u0000 </mrow>\u0000 <annotation>$$ Delta overline{nu} $$</annotation>\u0000 </semantics></math> = 8363 cm−1). The DFT configuration of the frontier molecular orbits in gas and solvated ground state (So) were compared with the solvated excited state (S1). The synthesized chromophore 6 was assessed for its antimicrobic properties against a group of bacterial and fungal strains. The minimum inhibitory concentration (MIC) values were determined using standard broth microdilution assay. Notably, chromophore 6 exhibited remarkable MIC values against Staphylococcus aureus and Escherichia coli (< 48 and < 118 μg/mL). Regarding the antibacterial effectiveness against both S. aureus and E. coli, molecular docking was performed to simulate their binding interactions with two protein structures, PDB:2eg7 and PDB:3u2k. The SwissADME study has been applied to explore the pharmacokinetic and pharmacodynamic characteristics of chromophore 6.\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"39 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142802888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Turn-On Fluorescence Probe Based on GSH-Protected Silver Nanoclusters for the Selective Detection of Pb2+ 基于gsh保护银纳米团簇的荧光探针选择性检测Pb2。

IF 3.2 4区化学

Luminescence Pub Date : 2024-12-09 DOI: 10.1002/bio.70050

Taiqun Yang, Lei Li, Cheng Ye, Chaoqun Ma, Hui Gao, Chun Zhu, Guoqing Chen

{"title":"A Turn-On Fluorescence Probe Based on GSH-Protected Silver Nanoclusters for the Selective Detection of Pb2+","authors":"Taiqun Yang, Lei Li, Cheng Ye, Chaoqun Ma, Hui Gao, Chun Zhu, Guoqing Chen","doi":"10.1002/bio.70050","DOIUrl":"10.1002/bio.70050","url":null,"abstract":"<div>\u0000 \u0000 Glutathione-protected silver nanoclusters (GSH-AgNCs) with good photostability were successfully synthesized through a one-pot heating process. The resultant GSH-AgNCs with an average diameter of ~1.6 nm exhibit red fluorescence (λem = 650 nm), and the fluorescence is excitation independent. Strong aggregation-induced emission (AIE) effect was observed in GSH-AgNCs, which can be induced by solvent and metal ions. In particular, a specific fluorescence enhancement was detected upon interaction with lead ions (Pb2+). A fluorescent probe was designed for off–on detection of Pb2+ based on as-synthesized GSH-AgNCs. Under the optimum parameters, two linear response intervals were found between the relative fluorescence intensity and the concentration of Pb2+ in the range of 10–250 μM (R2 = 0.9900) and 400–1000 μM (R2 = 0.9930). Significant fluorescence color evolutions observed under UV light, resulting in a semiquantitative visual detection of Pb2+. A possible detection mechanism based on the interaction between GSH-AgNCs and Pb2+ is discussed.\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"39 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142802874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication of a Random Laser by Embedding Copper Oxide Nanoparticles in Nonmetallic Host Media 在非金属介质中嵌入氧化铜纳米颗粒制备随机激光器。

IF 3.2 4区化学

Luminescence Pub Date : 2024-12-04 DOI: 10.1002/bio.70030

Noor E. Naji, Ali A. Aljubouri, Raid A. Ismail

{"title":"Fabrication of a Random Laser by Embedding Copper Oxide Nanoparticles in Nonmetallic Host Media","authors":"Noor E. Naji, Ali A. Aljubouri, Raid A. Ismail","doi":"10.1002/bio.70030","DOIUrl":"10.1002/bio.70030","url":null,"abstract":"<div>\u0000 \u0000 In this work, a simple, low-cost method for fabricating random gain media from Rhodamine B dye solution containing highly-pure CuO nanoparticles in transparent resin hosts is proposed. The spectroscopic characteristics of the dye solution samples were investigated with varying dye concentrations, along with nanoparticles of different particle sizes and concentrations. The fabricated samples were excited using two laser sources (405 and 530 nm) to record the fluorescence spectra. Our findings indicate that nanoparticles ranging in size from 15 to 78 nm may not have a significant impact on the fluorescence intensity or the peak wavelength at which maximum fluorescence occurs. Additionally, the fluorescence peak width, centered around 595 nm showed minimal sensitivity to the variation in particle size. The optimal concentration of nanoparticles in the dye solution was determined based on the optimum values of scattering spectral width and anisotropy parameter. Subsequently, the gain coefficient was calculated and correlated with these two parameters. It was found that the samples with the highest fluorescence intensity can be achieved using dye dissolved in transparent resin at a molar concentration of 5 × 10−5 M, containing 0.03–0.04 mg of CuO nanoparticles with a particle size of 78 nm.\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"39 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142781702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Involvement of CN Sites in Solvothermally Engineered Metal-Free Carbon Material From Weed Lantana camara for the Detection of Mercury Ions: Experimental and DFT Insights C - <s:1> - N位参与溶剂热工程无金属碳材料中的汞离子检测：实验和DFT见解。

IF 3.2 4区化学

Luminescence Pub Date : 2024-12-04 DOI: 10.1002/bio.70036

Sahil, Abhishek Soni, Jagadeesh Suriyaprakash, Raghubir Singh, Neeraj Gupta

{"title":"Involvement of CN Sites in Solvothermally Engineered Metal-Free Carbon Material From Weed Lantana camara for the Detection of Mercury Ions: Experimental and DFT Insights","authors":"Sahil, Abhishek Soni, Jagadeesh Suriyaprakash, Raghubir Singh, Neeraj Gupta","doi":"10.1002/bio.70036","DOIUrl":"10.1002/bio.70036","url":null,"abstract":"<div>\u0000 \u0000 Embarking on a journey to decipher the role of active sites in the detection and removal of toxic mercury(II) ions from polluted water, the surface of thermally engineered biomass derived carbon matrix NC-180 was customized with nitrogen atoms. The HR-TEM and XRD analyses revealed the amorphous nature of Lantana camara derived carbon material with small spherical flakes embedded in it. XPS analysis indicated the presence of pyrrolic, pyridinic, and graphitic N atoms, which was further confirmed by FT-IR analysis. The material shows quenching effect in presence of Hg2+ ions resulting in the “turn off” effect with a detection limit of 7.2 nM. The activity of NC-180 was recovered through “turn on” effect in the presence of L-cysteine. Furthermore, the mystery of binding of mercury(II) ions with N-sites is clarified through its comparison with other materials bearing sulfur and oxygen functional groups designated as AC-180, SC-180, and NSC-180. The conclusive evidence for efficient binding of nitrogen sites in NC-180 with mercury(II) ions is derived from various analyses, including 1H-NMR, FT-IR, XPS, and density functional theory. Notably, sustainability is achieved through utilization of toxic weed L. camara for the preparation of this selective carbon material for detection and adsorption of mercury(II) ions.\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"39 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142781703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploration of the Mechanism of Guaijaverin in Inhibiting Pancreatic Lipase Based on Multispectral Method, Molecular Docking, and Oral Lipid Tolerance Test in Rats 基于多光谱法、分子对接及大鼠口服脂质耐量试验的愈创黄嘌呤抑制胰脂肪酶作用机制探讨

IF 3.2 4区化学

Luminescence Pub Date : 2024-12-04 DOI: 10.1002/bio.70029

Xinyu Gao, Kaijie Jia, Hong Li, Shujun Liu, Yang Wang, Jinlong Tian, Xin Zhao, Pan Zhao

{"title":"Exploration of the Mechanism of Guaijaverin in Inhibiting Pancreatic Lipase Based on Multispectral Method, Molecular Docking, and Oral Lipid Tolerance Test in Rats","authors":"Xinyu Gao, Kaijie Jia, Hong Li, Shujun Liu, Yang Wang, Jinlong Tian, Xin Zhao, Pan Zhao","doi":"10.1002/bio.70029","DOIUrl":"10.1002/bio.70029","url":null,"abstract":"<div>\u0000 \u0000 This study aims to investigate the action mechanism of guaijaverin on pancreatic lipase from multiple perspectives and provide a theoretical basis for the search for new pancreatic lipase inhibitors. The inhibition of pancreatic lipase by guaijaverin was investigated through enzyme inhibition activity experiments, and the IC50 was calculated to determine the type of inhibition of guaijaverin. The mechanism of action was studied by measuring fluorescence, ultraviolet spectra, and circular dichroism. Molecular docking technology was used to explore the binding situation. In addition, in vivo oral lipid tolerance tests in rats were carried out to investigate the inhibitory effect of guaijaverin on pancreatic lipase. Guaijaverin inhibited pancreatic lipase up to 90.63%, confirming its excellent inhibitory ability. The inhibition type was noncompetitive inhibition in reversible inhibition. The multispectral experiments indicated that its quenching type was static quenching and guaijaverin changed the microenvironment and spatial conformation of pancreatic lipase. The molecular docking results showed that the minimum binding energy between the two compounds was −6.96 kcal/mol. In vivo experiments demonstrated that guaijaverin inhibits pancreatic lipase by reducing TG uptake in the body. Guaijaverin has excellent inhibitory effects on pancreatic lipase and has the potential to function as a pancreatic lipase inhibitor.\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"39 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142781701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0