Matrix Biology最新文献

Basement Membranes in Lung Development, Disease, and Repair. 基底膜在肺发育、疾病和修复中的作用。

IF 4.8 1区生物学

Matrix Biology Pub Date : 2025-07-28 DOI: 10.1016/j.matbio.2025.07.007

Matilda Thuringer, Roy Zent, Rachel Lennon, Erin J Plosa

引用次数: 0

Collagen Diversity in Human Skin: Aging, Wound Healing, And Disorders. 胶原蛋白在人类皮肤的多样性：老化，伤口愈合和疾病。

IF 4.8 1区生物学

Matrix Biology Pub Date : 2025-07-25 DOI: 10.1016/j.matbio.2025.07.006

Mélanie Salamito, Valérie Haydont, Hervé Pageon, Florence Ruggiero, Sarah Girardeau-Hubert

引用次数: 0

Drosophila Col4a1 Glycine mutations highlight allelic heterogeneity and mechanistic pleiotropy 果蝇Col4a1甘氨酸突变突出了等位基因异质性和机制多效性。

IF 4.5 1区生物学

Matrix Biology Pub Date : 2025-07-22 DOI: 10.1016/j.matbio.2025.07.005

Angus Nichols, Besaiz J. Sánchez-Sánchez, Stefania Marcotti, María-del-Carmen Díaz-de-la-Loza, Leonel C. Menezes, Tingfei Wang, Robert M. Johnson, Brian M. Stramer

{"title":"Drosophila Col4a1 Glycine mutations highlight allelic heterogeneity and mechanistic pleiotropy","authors":"Angus Nichols, Besaiz J. Sánchez-Sánchez, Stefania Marcotti, María-del-Carmen Díaz-de-la-Loza, Leonel C. Menezes, Tingfei Wang, Robert M. Johnson, Brian M. Stramer","doi":"10.1016/j.matbio.2025.07.005","DOIUrl":"10.1016/j.matbio.2025.07.005","url":null,"abstract":"<div><div>Collagen IV (Col4) is a heterotrimer containing a triple helical domain broken up by short interruptions. Mutation of Glycine residues within the Glycine-X-Y triple helical repeat leads to genetically dominant disease in humans that affects multiple organ systems. Mouse and cell culture-based models have revealed allelic heterogeneity, resulting in a range of Col4 secretion defects depending on the position of the mutation. However, genetic background also affects phenotypic severity, making it challenging to understand the precise underlying molecular mechanisms driving disease. Here, we characterize an allelic series of dominant temperature-sensitive <em>Drosophila</em> Glycine mutations to identify the potential molecular mechanisms driving phenotypic heterogeneity. Analysis of developmental viability at the non-permissive temperature revealed that mutations show a range of developmental lethality that is not correlated with their position within the triple helix nor with the degree of Col4 secretion defect. Backcrossing the series of fly lines led to increased lethality for almost all alleles, highlighting the presence of genetic modifiers, which paradoxically led to a reduction in secretion defects; this further suggests that defective secretion cannot explain the allelic heterogeneity in mutant viability. Analysis of the Col4 network surrounding the central nervous system (CNS) revealed that Glycine mutations can also affect basement membrane (BM) structure and alter its mechanical properties. Additionally, fluorescent tagging of a Glycine mutant variant showed that the mutated trimer is sufficiently secreted and can be incorporated into the network to dominantly affect BM organization. These data reveal that Col4 Glycine mutations can cause both secretion and direct BM network defects, suggesting that Col4-related pathologies may be mechanistically pleiotropic.</div></div>","PeriodicalId":49851,"journal":{"name":"Matrix Biology","volume":"140 ","pages":"Pages 113-122"},"PeriodicalIF":4.5,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144700161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ECM proteins shape topographical patterns in the basement membrane of Drosophila wing discs ECM蛋白在果蝇翅盘基底膜中形成地形模式

IF 4.5 1区生物学

Matrix Biology Pub Date : 2025-07-15 DOI: 10.1016/j.matbio.2025.06.003

K. Yanín Guerra Santillán, Christian Dahmann, Elisabeth Fischer-Friedrich

{"title":"ECM proteins shape topographical patterns in the basement membrane of Drosophila wing discs","authors":"K. Yanín Guerra Santillán, Christian Dahmann, Elisabeth Fischer-Friedrich","doi":"10.1016/j.matbio.2025.06.003","DOIUrl":"10.1016/j.matbio.2025.06.003","url":null,"abstract":"<div><div>The basal surface of epithelial tissues is attached to a thin network of macromolecules known as the basement membrane. The core components of the basement membrane — Collagen IV, Laminin, Perlecan, and Nidogen — are conserved extracellular matrix (ECM) proteins across species. However, the topography of basement membranes and the contribution of individual core components to its establishment remain poorly understood. Here, we used AFM-aided PeakForce tapping to analyze the topography of the basement membrane of <em>Drosophila</em> larval wing discs. We identified a self-affine surface topography, appearing structurally similar across multiple scales. Further, the topography is characterized by thin fiber-like structures that are intermittently aligned with a preferred orientation along the anterior-posterior axis. During larval development, the amplitude of surface patterns overall decreases, whereas the abundance of basement membrane components increases. Using targeted knockdown experiments, we show that Collagen IV is essential for the formation of fiber-like structures, while Laminin and Collagen IV appear to smooth or level out large-scale groove-like patterns. In contrast, Nidogen contributes to the maintenance of these grooves, and Perlecan increases surface pattern amplitudes at all length scales. Our findings reveal distinct topographical features in the basement membrane, whose amplitude and organization depend on its specific molecular composition.</div></div>","PeriodicalId":49851,"journal":{"name":"Matrix Biology","volume":"140 ","pages":"Pages 78-87"},"PeriodicalIF":4.5,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144655501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of extracellular space and matrix remodeling in cardiac amyloidosis 细胞外空间和基质重塑在心脏淀粉样变性中的作用

IF 4.5 1区生物学

Matrix Biology Pub Date : 2025-07-14 DOI: 10.1016/j.matbio.2025.07.004

Francesca Lavatelli , Loredana Marchese , Palma Patrizia Mangione , Sara Raimondi , Diana Canetti , Guglielmo Verona , Lucia Venneri , Eloisa Arbustini , Laura Obici , Alessandra Corazza , Vittorio Bellotti , Sofia Giorgetti

引用次数: 0

Building basement membranes with computational approaches 用计算方法构建基底膜。

IF 4.5 1区生物学

Matrix Biology Pub Date : 2025-07-09 DOI: 10.1016/j.matbio.2025.07.001

Alana Stevenson Harris , Rachel Lennon , Jean-Marc Schwartz

{"title":"Building basement membranes with computational approaches","authors":"Alana Stevenson Harris , Rachel Lennon , Jean-Marc Schwartz","doi":"10.1016/j.matbio.2025.07.001","DOIUrl":"10.1016/j.matbio.2025.07.001","url":null,"abstract":"<div><div>Basement membranes (BMs) are dense extracellular matrix scaffolds that support cells. Their composition, structure and dynamic regulation are vital for tissue health and altered in human disease. The expansion of experimental and analytical techniques has generated large multiomic datasets that include BM components; however, the organising principles of BM component assembly and the regulation of BMs remain poorly understood. There are over 160 curated BM proteins, including core, ubiquitous components such as type IV collagen and laminin isoforms, as well as tissue-restricted components, and there is increasing experimental evidence of BM protein-protein interactions. Here we describe and compare multiomic, protein-protein interaction, and BM curation databases and discuss the application of systems biology approaches including network analysis, Boolean networks and Ordinary Differential Equations to integrate data and model BM organisation. Applying computational modelling strategies to BM datasets may reveal unknown organising principles of BM assembly and regulation and predict mechanisms of dysregulation in BM-associated diseases.</div></div>","PeriodicalId":49851,"journal":{"name":"Matrix Biology","volume":"140 ","pages":"Pages 88-99"},"PeriodicalIF":4.5,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144621006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Around the collagen triple helix: an introduction to studying associated genetic and acquired diseases 围绕胶原蛋白三螺旋：介绍研究相关的遗传和获得性疾病。

IF 4.5 1区生物学

Matrix Biology Pub Date : 2025-07-07 DOI: 10.1016/j.matbio.2025.07.003

Sergei P. Boudko

{"title":"Around the collagen triple helix: an introduction to studying associated genetic and acquired diseases","authors":"Sergei P. Boudko","doi":"10.1016/j.matbio.2025.07.003","DOIUrl":"10.1016/j.matbio.2025.07.003","url":null,"abstract":"<div><div>The triple helix structure of collagen is the most abundant motif found in our bodies. It is believed to have emerged during the transition from unicellular to multicellular animal organisms, known as metazoans, and has evolved into various proteins that contribute to the development and function of diverse animal tissues, organs, and systems. Once synthesized, these collagenous proteins undergo post-translational modifications and proper folding inside the cell, after which they primarily function outside the cell. Over 80 collagenous proteins are categorized into two main groups: collagens and collagen-like proteins. However, the distinction between these groups is not clearly defined. Within these categories, there are various types of proteins, including soluble proteins, transmembrane proteins, and those that form the extracellular matrix. Multiple genetic diseases highlight the significance of collagenous proteins, which can be affected by defects in their primary structure, post-translational modifications, or complete loss. While fixing the gene defect may seem like a straightforward solution, we currently lack the capability to do so. Moreover, acquired diseases caused or accompanied by adverse processes in the collagen triple helix are generally not suitable for gene therapy at all. Understanding the pathogenicity of a defective polypeptide chain can provide valuable insights into strategies for mitigating negative consequences for both genetic and acquired diseases. This review highlights the current state of research in the collagen triple helix field, offering insights into how to study specific defects and deepen our understanding of their underlying pathogenic mechanisms.</div></div>","PeriodicalId":49851,"journal":{"name":"Matrix Biology","volume":"140 ","pages":"Pages 43-58"},"PeriodicalIF":4.5,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144602117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Collagen IV biosynthesis: Intracellular choreography of post-translational modifications 胶原IV型生物合成：翻译后修饰的细胞内编排。

IF 4.5 1区生物学

Matrix Biology Pub Date : 2025-07-04 DOI: 10.1016/j.matbio.2025.07.002

Yoshihiro Ishikawa , Rachel Lennon , Federico Forneris , Johanna Myllyharju , Antti M. Salo

{"title":"Collagen IV biosynthesis: Intracellular choreography of post-translational modifications","authors":"Yoshihiro Ishikawa , Rachel Lennon , Federico Forneris , Johanna Myllyharju , Antti M. Salo","doi":"10.1016/j.matbio.2025.07.002","DOIUrl":"10.1016/j.matbio.2025.07.002","url":null,"abstract":"<div><div>Collagen IV, an essential and evolutionarily conserved component of basement membranes, is one of the most extensively post-translationally modified proteins. Despite substantial research on fibrillar collagen biosynthesis, our understanding of collagen IV biosynthesis, including its post-translational modifications (PTMs), remains limited. Most PTMs occur intracellularly, primarily within the endoplasmic reticulum (ER). In this review, we examine the molecular ensemble that orchestrates collagen IV biosynthesis in the ER, highlighting the complex interplay between prolyl and lysyl hydroxylases, glycosyltransferases, and molecular chaperones. Furthermore, we discuss how defects in collagen IV and its PTMs contribute to various human pathologies, including Gould and Alport syndromes, fibrosis, and cancer. Understanding collagen IV PTMs is crucial for elucidating the molecular basis of these diseases and improving targeted treatments. By reviewing our knowledge of collagen IV biosynthesis, we illustrate how this evolutionarily conserved yet highly specialized molecular biosynthesis ensemble supports the diverse functions of collagen IV in health and disease.</div></div>","PeriodicalId":49851,"journal":{"name":"Matrix Biology","volume":"140 ","pages":"Pages 59-77"},"PeriodicalIF":4.5,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144576776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paracrine regulations of IFN-γ secreting CD4+ T cells by lumican and biglycan are protective in allergic contact dermatitis lumican和biglycan对IFN-γ分泌CD4+ T细胞的旁分泌调节在过敏性接触性皮炎中具有保护作用

IF 4.5 1区生物学

Matrix Biology Pub Date : 2025-06-14 DOI: 10.1016/j.matbio.2025.06.002

George Maiti , Jihane Frikeche , Cynthia Loomis , Michael Cammer , Stephanie L Eichman , Shukti Chakravarti

{"title":"Paracrine regulations of IFN-γ secreting CD4+ T cells by lumican and biglycan are protective in allergic contact dermatitis","authors":"George Maiti , Jihane Frikeche , Cynthia Loomis , Michael Cammer , Stephanie L Eichman , Shukti Chakravarti","doi":"10.1016/j.matbio.2025.06.002","DOIUrl":"10.1016/j.matbio.2025.06.002","url":null,"abstract":"<div><div>Allergic contact dermatitis (ACD) is a delayed-type IV hypersensitivity response driven by innate and adaptive immune cells. While specific immune regulations of these cell types are amply elucidated, their regulations by extracellular matrix (ECM) components and T cell mediated adaptive immunity in ACD remains unclear. Lumican and biglycan are ECM proteoglycans abundant in the dermis and lymph node, known to regulate innate immune myeloid cells, but have not been investigated in lymphoid cell regulations in ACD. By immunohistology we localized lumican and biglycan in skin biopsies of psoriatic patients. Using wild type (WT), lumican and biglycan knockout mice, we investigated CD4<sup>+</sup>T cell infiltration, activation and proliferation in the skin and draining lymph node (dLN) of contact hypersensitivity (CHS)-challenged mice by immunohistochemistry and flow cytometry. We used the OT-II adoptive transfer model to test antigen specific CD4<sup>+</sup>T cell activation. We assessed interactions of the proteoglycans with LFA-1 on T cells by confocal microscopy. Compared to WTs, the knockouts showed severe ear inflammation, with increased CD4<sup>+</sup>T cells infiltration in the dermis. CHS-challenged knockout mice dLN showed increased T-bet, STAT1 and -STAT4 signaling, indicating enhanced Th1 commitment and proliferation. We found that WT lymph node fibroblastic reticular cells (FRCs) secrete lumican, biglycan and decorin, a related proteoglycan, while none are expressed by naive or activated T cells. Lumican and biglycan interact with LFA-1 on T cell surfaces, and <em>in vitro</em> all three proteoglycans suppress CD4<sup>+</sup>T cell activation. Secreted by dLN FRCs, lumican, biglycan, and possibly decorin interact with LFA-1 on CD4<sup>+</sup>T cells to restrict their activation and reduce dermatitis severity.</div></div>","PeriodicalId":49851,"journal":{"name":"Matrix Biology","volume":"140 ","pages":"Pages 27-42"},"PeriodicalIF":4.5,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144289819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A complex relationship between the architecture of the basement membrane, its mechanical properties, and its ability to shape the Drosophila egg 基膜的结构，它的机械特性，和它塑造果蝇卵的能力之间的复杂关系

IF 4.5 1区生物学

Matrix Biology Pub Date : 2025-06-13 DOI: 10.1016/j.matbio.2025.06.001

Mitchell T. Anderson , Sally Horne-Badovinac

{"title":"A complex relationship between the architecture of the basement membrane, its mechanical properties, and its ability to shape the Drosophila egg","authors":"Mitchell T. Anderson , Sally Horne-Badovinac","doi":"10.1016/j.matbio.2025.06.001","DOIUrl":"10.1016/j.matbio.2025.06.001","url":null,"abstract":"<div><div>Basement membranes (BMs) are planar extracellular matrices that line the basal surfaces of epithelia and are essential components of most organs. During development, BMs can also play instructive roles in shaping the tissues to which they belong, but how they do so is incompletely understood. The <em>Drosophila</em> egg chamber has become a premier system to study this aspect of BM biology due to the ostensible simplicity of the BM’s role in morphogenesis. The prevailing model posits that the egg chamber’s outer layer of epithelial cells creates a symmetric stiffness gradient in the surrounding BM that preferentially channels egg chamber growth along one axis to create the elongated shape of the egg. There is evidence that the stiffening of the BM depends, in part, on a polarized array of fibrils that form within the BM network, and yet the exact role the BM fibrils play in egg chamber elongation has remained unclear. Here, we use genetic conditions that abrogate fibril formation to different extents to probe the relationship between the BM’s fibril content, its mechanical properties, and the shape of the egg. The results of these experiments are consistent with a model in which BM fibrils influence egg shape by directly augmenting the mechanical properties of the BM. However, we then examine a final genetic condition that does not fit this simple narrative. We propose that the role of the BM in conferring final egg shape is more complicated than previously thought and that some approaches used to study this role should be re-evaluated for their efficacy.</div></div>","PeriodicalId":49851,"journal":{"name":"Matrix Biology","volume":"140 ","pages":"Pages 16-26"},"PeriodicalIF":4.5,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144289818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0