Zeitschrift Fur Naturforschung Section C-A Journal of Biosciences最新文献_第9页

Stability of proteins involved in initiation of DNA replication in UV damaged human cells. 紫外线损伤人体细胞中DNA复制起始蛋白的稳定性。

IF 2 4区生物学

Zeitschrift Fur Naturforschung Section C-A Journal of Biosciences Pub Date : 2021-08-02 Print Date: 2022-03-28 DOI: 10.1515/znc-2020-0286

Boyka Borisova Anachkova, Vera Lyubchova Djeliova

{"title":"Stability of proteins involved in initiation of DNA replication in UV damaged human cells.","authors":"Boyka Borisova Anachkova, Vera Lyubchova Djeliova","doi":"10.1515/znc-2020-0286","DOIUrl":"https://doi.org/10.1515/znc-2020-0286","url":null,"abstract":"The protein stability of the initiation factors Orc2, Orc3, Orc4, and Cdc6 was analyzed after UV light exposure in two human cell lines. In the cell line with higher repair capacity, HEK 293, no changes in the cell cycle distribution or in the protein levels of the investigated factors were detected. In HeLa cells that are characterized by lower repair capacity, UV irradiation caused a reduction of the levels of Cdc6, Orc2 and Orc3, but not of Orc4 or triggered apoptosis. The appearance of the truncated 49 kDa form of Cdc6 suggested the involvement of the caspase pathway in the degradation of the proteins. Reduced protein levels of Cdc6 were detected in UV damaged HeLa cells in which the apoptotic process was blocked with the caspase inhibitor Z-VAD-fmk, indicating that the degradation of Cdc6 is mediated by the proteasome pathway instead. In the presence of caffeine, an inhibitor of the cell cycle checkpoint kinases, Cdc6 was stabilized, demonstrating that its degradation is controlled by the DNA damage cell cycle checkpoint. We conclude that in response to DNA damage, the activation of origins of replication can be prevented by the degradation of Cdc6, most likely through the proteasome pathway.","PeriodicalId":49344,"journal":{"name":"Zeitschrift Fur Naturforschung Section C-A Journal of Biosciences","volume":"77 3-4","pages":"113-123"},"PeriodicalIF":2.0,"publicationDate":"2021-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39263801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monascin and monascinol, azaphilonoid pigments from Mortierella polycephala AM1: in silico and in vitro targeting of the angiogenic VEGFR2 kinase. Monascin和monascinol，来自多头孢杆菌AM1的azaphilonoid色素:在硅和血管生成VEGFR2激酶的体外靶向。

IF 2 4区生物学

Zeitschrift Fur Naturforschung Section C-A Journal of Biosciences Pub Date : 2021-07-16 Print Date: 2022-01-27 DOI: 10.1515/znc-2021-0095

Mohamed Shaaban, Mohammad Magdy El-Metwally, Amal A I Mekawey, Ahmed B Abdelwahab, Maha M Soltan

{"title":"Monascin and monascinol, azaphilonoid pigments from Mortierella polycephala AM1: in silico and in vitro targeting of the angiogenic VEGFR2 kinase.","authors":"Mohamed Shaaban, Mohammad Magdy El-Metwally, Amal A I Mekawey, Ahmed B Abdelwahab, Maha M Soltan","doi":"10.1515/znc-2021-0095","DOIUrl":"https://doi.org/10.1515/znc-2021-0095","url":null,"abstract":"Abstract The fungus, Mortierella polycephala is one of the most productive sources of anticancer bioactive compounds namely those of pigment nature. During our investigation of the produced bioactive metabolites by the terrestrial M. polycephala AM1 isolated from Egyptian poultry feather waste, two main azaphilonoid pigments, monascin (1) and monascinol (2) were obtained as major products; their structures were identified by 1D (1H&13C) and 2D (1H–1H COSY, HMBC) NMR and HRESI-MS spectroscopic data. Biologically, cytotoxic activities of these compounds were broadly studied compared with the fungal extract. To predict the biological target for the presumed antitumor activity, an in silico study was run toward three proteins, topoisomerase IIα, topoisomerase IIβ, and VEGFR2 kinase. Monascinol (2) was expected to be moderately active against VEGFR2 kinase without any anticipated inhibition toward topo II isoforms. The in vitro study confirmed the docked investigation consistently and introduced monascinol (2) rather than its counterpart (1) as a potent inhibitor to the tested VEGFR2 kinase. Taxonomically, the fungus was identified using morphological and genetic assessments.","PeriodicalId":49344,"journal":{"name":"Zeitschrift Fur Naturforschung Section C-A Journal of Biosciences","volume":"77 1-2","pages":"11-19"},"PeriodicalIF":2.0,"publicationDate":"2021-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39187304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Renewable mycelium based composite - sustainable approach for lignocellulose waste recovery and alternative to synthetic materials - a review. 基于可再生菌丝体的复合材料——木质纤维素废物可持续回收和替代合成材料的方法综述。

IF 2 4区生物学

Zeitschrift Fur Naturforschung Section C-A Journal of Biosciences Pub Date : 2021-07-13 Print Date: 2021-11-25 DOI: 10.1515/znc-2021-0040

Galena V Angelova, Mariya S Brazkova, Albert I Krastanov

{"title":"Renewable mycelium based composite - sustainable approach for lignocellulose waste recovery and alternative to synthetic materials - a review.","authors":"Galena V Angelova, Mariya S Brazkova, Albert I Krastanov","doi":"10.1515/znc-2021-0040","DOIUrl":"https://doi.org/10.1515/znc-2021-0040","url":null,"abstract":"The agricultural waste with lignocellulose origin is considered to be one of the major environmental pollutants which, because of their high nutritional value, represent an extremely rich resource with significant potential for the production of value added bio-products. This review discusses the applications of higher fungi to upcycle abundant agricultural by-products into more sustainable materials and to promote the transition to a circular economy. It focuses on the main factors influencing the properties and application of mycelium composites - the feedstock, the basidiomycete species and their interaction with the feedstock. During controlled solid state cultivation on various lignocellulose substrates, the basidiomycetes of class Agaricomycetes colonize their surfaces and form a three-dimensional mycelium net. Fungal mycelium secretes enzymes that break down lignocellulose over time and are partially replaced by mycelium. The mycelium adheres to the residual undegraded substrates resulting in the formation of a high-mechanical-strength bio-material called a mycelium based bio-composite. The mycelium based bio-composites are completely natural, biodegradable and can be composted after their cycle of use is completed. The physicochemical, mechanical, and thermodynamic characteristics of mycelium based bio-composites are competitive with those of synthetic polymers and allow them to be successfully used in the construction, architecture, and other industries.","PeriodicalId":49344,"journal":{"name":"Zeitschrift Fur Naturforschung Section C-A Journal of Biosciences","volume":"76 11-12","pages":"431-442"},"PeriodicalIF":2.0,"publicationDate":"2021-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znc-2021-0040","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39177340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Isolation and characterization of chemical constituents from Chaerophyllum bulbosum roots and their enzyme inhibitory and antioxidant effects. 毛竹根化学成分的分离鉴定及其酶抑制和抗氧化作用。

IF 2 4区生物学

Zeitschrift Fur Naturforschung Section C-A Journal of Biosciences Pub Date : 2021-07-06 Print Date: 2022-01-27 DOI: 10.1515/znc-2021-0119

Gülsen Tel-Çayan, Ebru Deveci, Zeynep Molo, Mehmet Emin Duru, Mehmet Öztürk

{"title":"Isolation and characterization of chemical constituents from Chaerophyllum bulbosum roots and their enzyme inhibitory and antioxidant effects.","authors":"Gülsen Tel-Çayan, Ebru Deveci, Zeynep Molo, Mehmet Emin Duru, Mehmet Öztürk","doi":"10.1515/znc-2021-0119","DOIUrl":"https://doi.org/10.1515/znc-2021-0119","url":null,"abstract":"Isolation and bioactive effects of the roots of Chaerophyllum bulbosum L. were firstly investigated herein. Enzyme (acetylcholinesterase, butyrylcholinesterase, urease, α-amylase, α-glucosidase, and tyrosinase) inhibitory effects of C. bulbosum root extracts were tested. Three known compounds, n-heptadecanyl eicosanoate (1), stigmasterol (2), and β-sitosterol-3-O-β-d-glucopyranoside (3) were isolated from C. bulbosum. Antioxidant and enzyme inhibitory effects of isolated compounds were investigated. The hexane extract (IC50: 349.58 ± 0.06 μg/mL) displayed a higher α-glucosidase inhibitory effect than the standard (IC50: 378.66 ± 0.14 μg/mL). The best inhibitory effect was found in compound 2 on AChE (46.40 ± 0.31%), BChE (56.41 ± 0.54%), and urease (92.47 ± 0.11%); compound 1 on α-amylase (22.27 ± 0.61%); and compound 3 on α-glucosidase (12.43 ± 0.25%) and tyrosinase (19.00 ± 0.16%). All isolated compounds showed moderate antioxidant effects in all assays. This study contributes to the therapeutic uses of Chaerophyllum roots and emphasizes the value of C. bulbosum species for the development of novel therapeutic agents.","PeriodicalId":49344,"journal":{"name":"Zeitschrift Fur Naturforschung Section C-A Journal of Biosciences","volume":"77 1-2","pages":"1-9"},"PeriodicalIF":2.0,"publicationDate":"2021-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39152326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Novel Ag(I)-NHC complex: synthesis, in vitro cytotoxic activity, molecular docking, and quantum chemical studies. 新型Ag(I)-NHC复合物:合成、体外细胞毒活性、分子对接和量子化学研究。

IF 2 4区生物学

Zeitschrift Fur Naturforschung Section C-A Journal of Biosciences Pub Date : 2021-07-06 Print Date: 2022-01-27 DOI: 10.1515/znc-2021-0130

Goncagül Serdaroğlu, Neslihan Şahin, Serap Şahin-Bölükbaşı, Elvan Üstün

{"title":"Novel Ag(I)-NHC complex: synthesis, in vitro cytotoxic activity, molecular docking, and quantum chemical studies.","authors":"Goncagül Serdaroğlu, Neslihan Şahin, Serap Şahin-Bölükbaşı, Elvan Üstün","doi":"10.1515/znc-2021-0130","DOIUrl":"https://doi.org/10.1515/znc-2021-0130","url":null,"abstract":"The importance of organometallic complexes in cancer biology has attracted attention in recent years. In this paper, we look for the in vitro cytotoxic capability of novel benzimidazole-based N-heterocyclic carbene (NHC) precursor (1) and its Ag(I)-NHC complex (2). For this purpose, these novel Ag(I)-NHC complex (2) was characterized by spectroscopic techniques (1H, 13C{1H} nuclear magnetic resonance (NMR), and Fourier-transform infrared spectroscopy (FT-IR)). Then, in vitro cytotoxic activities of NHC precursor (1) and Ag(I)-NHC complex (2) were investigated against MCF-7, MDA-MB-231 human breast, DU-145 prostate cancer cells, and L-929 healthy cells using MTT assay for 24, 48, and 72 h incubation times. Ag(I)-NHC complex (2) showed promising in vitro cytotoxic activity against all cell lines for three incubation times, with IC50 values lower than 5 µM. It was also determined that (NHC) precursor (1) were lower in vitro cytotoxic activity than Ag(I)-NHC complex (2) against all cell lines. Selectivity indexes (SIs) of Ag(I)-NHC complex (2) against cancer cells were found higher than 2 for 24 and 48 h incubation time. Besides, the electronic structure and spectroscopic data of the newly synthesized precursor and its Ag-complex have been supported by density functional theory (DFT) calculations and molecular docking analysis. After, the anticancer activity of these compounds has been discussed considering the results of the frontier molecular orbital analysis. We hope that the obtained results from the experiments and computational tools will bring a new perspective to cancer research in terms of supported by quantum chemical calculations.","PeriodicalId":49344,"journal":{"name":"Zeitschrift Fur Naturforschung Section C-A Journal of Biosciences","volume":"77 1-2","pages":"21-36"},"PeriodicalIF":2.0,"publicationDate":"2021-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znc-2021-0130","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39152330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Bioactive compounds from Matricaria chamomilla: structure identification, in vitro antiproliferative, antimigratory, antiangiogenic, and antiadenoviral activities. 洋甘菊的生物活性化合物:结构鉴定、体外抗增殖、抗迁移、抗血管生成和抗腺病毒活性。

IF 2 4区生物学

Zeitschrift Fur Naturforschung Section C-A Journal of Biosciences Pub Date : 2021-06-22 Print Date: 2022-03-28 DOI: 10.1515/znc-2021-0083

Mohamed Shaaban, Ali M El-Hagrassi, Abeer F Osman, Maha M Soltan

{"title":"Bioactive compounds from Matricaria chamomilla: structure identification, in vitro antiproliferative, antimigratory, antiangiogenic, and antiadenoviral activities.","authors":"Mohamed Shaaban, Ali M El-Hagrassi, Abeer F Osman, Maha M Soltan","doi":"10.1515/znc-2021-0083","DOIUrl":"https://doi.org/10.1515/znc-2021-0083","url":null,"abstract":"During our exploring the anticancer activity of some medicinal plants and their major metabolites, the aerial parts of the Egyptian Matricaria chamomilla (flowers and stems) were studied. GC-MS analysis of the organic soluble extracts of the flowers and stems fractions revealed the presence of 43 and 45 compounds, respectively. Individual chromatographic purification of the flowers and stems' extracts afforded three major compounds. Structures of these compounds were identified by 1D- and 2D-NMR and HRESI-MS spectroscopic data as bisabolol oxide A (1) and (E)-tonghaosu (2) (as mixture of ratio 2:1) from the flowers extract, meanwhile apigenin-7-β-d-glucoside (3) from the stems fraction. Biologically, the chamomile extracts announced significant antiproliferative activities exceeded in potency by ∼1.5 fold in case of the stem, recording GI50 13.16 and 17.04 μg/mL against Caco-2 and MCF-7, respectively. Both fractions were approximately equipotent against the migration of the same cell type down to 10 μg/mL together, compounds 1, 2 but not 3, showed considerable growth inhibition of the same cells at GI50 13.36 and 11.83 μg/mL, respectively. Interestingly, they were able to suppress Caco-2 colon cancer cells migration at 5.8 μg/mL and potently inactivate the VEGFR2 angiogenic enzyme (1.5-fold relative to sorafenib. The obtained compounds and corresponding chamomile extracts were evaluated against Adeno-7 virus, revealing that both chamomiles' extracts (flowers and stems) and their corresponding obtained compounds (1-3) were potent in their depletion to the Adeno 7 infectivity titer, however, the flower extract and compounds 1-2 were more effective than those of the stem extract and its end-product (3).","PeriodicalId":49344,"journal":{"name":"Zeitschrift Fur Naturforschung Section C-A Journal of Biosciences","volume":"77 3-4","pages":"85-94"},"PeriodicalIF":2.0,"publicationDate":"2021-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39370671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Seroprevalence of SARS-CoV-2 in Pakistan: an update on epidemiological trends. 巴基斯坦SARS-CoV-2血清流行率:流行病学趋势最新情况

IF 2 4区生物学

Zeitschrift Fur Naturforschung Section C-A Journal of Biosciences Pub Date : 2021-06-22 Print Date: 2021-09-27 DOI: 10.1515/znc-2020-0291

Muhammad Waqar, Braira Wahid, Muhammad Idrees, Muhammad Ali, Zobaria Rehman

引用次数: 1

Physicochemical, microbiological, and sensory characteristics of probiotic Bulgarian yoghurts obtained by ultrafiltration of goat's milk. 超滤羊奶所得益生菌保加利亚酸奶的理化、微生物学和感官特性。

IF 2 4区生物学

Zeitschrift Fur Naturforschung Section C-A Journal of Biosciences Pub Date : 2021-06-21 Print Date: 2021-11-25 DOI: 10.1515/znc-2021-0064

Mariya Dushkova, Siyka Kodinova, Zapryana Denkova, Velichka Yanakieva, Nikolay Dimitrov Menkov

{"title":"Physicochemical, microbiological, and sensory characteristics of probiotic Bulgarian yoghurts obtained by ultrafiltration of goat's milk.","authors":"Mariya Dushkova, Siyka Kodinova, Zapryana Denkova, Velichka Yanakieva, Nikolay Dimitrov Menkov","doi":"10.1515/znc-2021-0064","DOIUrl":"https://doi.org/10.1515/znc-2021-0064","url":null,"abstract":"The purpose of this study was to investigate the microbiological (number of viable lactic acid bacteria and bifidobacteria), physicochemical (dry matter, protein and fat contents, titratable acidity, and pH), and sensory characteristics (appearance of coagulum, taste and aroma, structure at cutting, color, and consistency at shattering) of probiotic Bulgarian yoghurts obtained by ultrafiltration of goat's milk. These yoghurts were obtained using volume reduction ratios of 2 and 3 with the probiotic starters MZ2f, MZ2f + Bifidobacterium bifidum BB - 87, and MZ2f + Lactobacillus acidophilus LAB - 8. The increase in the level of the concentration by ultrafiltration led to an increase in the dry matter, protein and fat contents of the yoghurts, in the number of lactic acid bacteria and titratable acidity, and to a decrease in the pH. The twofold concentration by ultrafiltration resulted in a higher number of lactic acid bacteria in comparison with yoghurts made without ultrafiltration, and with better sensory characteristics compared to yoghurts without and with threefold ultrafiltration. The higher number of viable cells and better sensory characteristics were obtained for yoghurts with MZ2f + Bifidobacterium bifidum BB - 87 and MZ2f + Lactobacillus acidophilus LAB - 8 in comparison with MZ2f alone.","PeriodicalId":49344,"journal":{"name":"Zeitschrift Fur Naturforschung Section C-A Journal of Biosciences","volume":"76 11-12","pages":"481-489"},"PeriodicalIF":2.0,"publicationDate":"2021-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39253743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Antimicrobial synergism and antibiofilm activities of Pelargonium graveolens, Rosemary officinalis, and Mentha piperita essential oils against extreme drug-resistant Acinetobacter baumannii clinical isolates. 天竺葵、迷迭香和薄荷精油对极端耐药鲍曼不动杆菌临床分离株的抗菌增效作用及抗菌膜活性研究

IF 2 4区生物学

Zeitschrift Fur Naturforschung Section C-A Journal of Biosciences Pub Date : 2021-06-17 Print Date: 2022-03-28 DOI: 10.1515/znc-2021-0079

Ayse Humeyra Taskin Kafa, Rukiye Aslan, Cem Celik, Mursit Hasbek

{"title":"Antimicrobial synergism and antibiofilm activities of Pelargonium graveolens, Rosemary officinalis, and Mentha piperita essential oils against extreme drug-resistant Acinetobacter baumannii clinical isolates.","authors":"Ayse Humeyra Taskin Kafa, Rukiye Aslan, Cem Celik, Mursit Hasbek","doi":"10.1515/znc-2021-0079","DOIUrl":"https://doi.org/10.1515/znc-2021-0079","url":null,"abstract":"Rosemary officinalis L., Pelargonium graveolens L., and Mentha piperita L., essential oils are used by complementary medicine specialists simultaneously with traditional antibiotics for treatment purposes. The chemical composition of essential oils was analyzed by the gas chromatography-mass spectrometry method. In vitro antibacterial and antibiofilm activities of the essential oils were tested against extreme drug-resistant (XDR) colistin-resistant and colistin susceptible Acinetobacter baumannii clinical strains. The synergistic activities between essential oils and colistin antibiotics were investigated by the checkerboard method. The highest antibacterial effect was detected in mint essential oil (2.5-5 μl/ml), followed by pelargonium essential oil (5-20 μl/ml) and rosemary essential oil (5-20 μl/ml). The combination of rosemary essential oil or pelargonium essential oil with colistin showed strong synergistic activity in most of the bacterial strains tested (fractional inhibitory concentration index ≤ 0.5; synergy). As a result of the combination of mint essential oil and colistin, an indifferent effect was observed in only two bacterial strains, and other strains could not be evaluated. No antagonistic effects were observed in any of the tested essential oils. As a result of the effectiveness of the combination, the minimum inhibitory concentration (MIC) values of colistin in XDR-A. baumannii clinical isolates decreased 2-32 fold. Additionally, the sub-MIC concentration of essential oils exhibited an inhibitory effect (48-90%) against the biofilm layer of tested A. baumannii strains.","PeriodicalId":49344,"journal":{"name":"Zeitschrift Fur Naturforschung Section C-A Journal of Biosciences","volume":"77 3-4","pages":"95-104"},"PeriodicalIF":2.0,"publicationDate":"2021-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39244276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

A synergy interaction of artocarpin and tetracycline against Pseudomonas aeruginosa and its mechanism of action on membrane permeability. artocarpin和四环素对铜绿假单胞菌的协同作用及其对膜通透性的作用机制。

IF 2 4区生物学

Zeitschrift Fur Naturforschung Section C-A Journal of Biosciences Pub Date : 2021-06-09 Print Date: 2022-01-27 DOI: 10.1515/znc-2021-0076

Abdi Wira Septama, Eldiza Puji Rahmi, Lucia Dwi Antika, Rizna Triana Dewi, Amit Jaisi

{"title":"A synergy interaction of artocarpin and tetracycline against Pseudomonas aeruginosa and its mechanism of action on membrane permeability.","authors":"Abdi Wira Septama, Eldiza Puji Rahmi, Lucia Dwi Antika, Rizna Triana Dewi, Amit Jaisi","doi":"10.1515/znc-2021-0076","DOIUrl":"https://doi.org/10.1515/znc-2021-0076","url":null,"abstract":"The emergence of antibacterial resistance has significantly increased. Pseudomonas aeruginosa is associated with nosocomial infection and difficult to control. Artocarpin, a flavonoid from Artocarpus heterophyllus Lam. exhibits several pharmacological properties including antibacterial. The study was performed to evaluate interaction between artocarpin and antibiotics including tetracycline against P. aeruginosa. Its mechanism of action on membrane permeability was also investigated. Broth microdilution was conducted for the susceptibility assay. The interaction of artocarpin and antibiotics was evaluated using checkerboard method, the effect on alteration of membrane cell was investigated using bacteriolysis and the released of 260 nm materials. Artocarpin showed moderate to weak activity against the Gram-negative bacteria including P. aeruginosa with MIC values in the range of 31.25-250 μg/mL. A synergistic effect against P. aeruginosa was produced by the combination of artocarpin (31.25 μg/mL) and tetracycline (1.95 μg/mL) with FICI of 0.37. The time-killing assay showed that artocarpin enhance the antibacterial activity of tetracycline against P. aeruginosa by completely inhibiting the bacterial growth. Additionally, the mixture of artocarpin (31.25 μg/mL) and tetracycline (1.95 μg/mL) disrupted membrane permeability and lead to cell death. These results proposed that the combination of artocarpin and tetracycline may be used to overcome P. aeruginosa infection.","PeriodicalId":49344,"journal":{"name":"Zeitschrift Fur Naturforschung Section C-A Journal of Biosciences","volume":"77 1-2","pages":"57-63"},"PeriodicalIF":2.0,"publicationDate":"2021-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39093466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2