Biopreservation and Biobanking最新文献

{"title":"Feasibility and Comparison Study of Fecal Sample Collection Methods in Healthy Volunteers and Solid Organ Transplant Recipients Using 16S rRNA and Metagenomics Approaches.","authors":"Sunil M Kurian,&nbsp;Skyler Gordon,&nbsp;Bethany Barrick,&nbsp;Manoj N Dadlani,&nbsp;Brian Fanelli,&nbsp;Jenny B Cornell,&nbsp;Steven R Head,&nbsp;Christopher L Marsh,&nbsp;Jamie Case","doi":"10.1089/bio.2020.0032","DOIUrl":"https://doi.org/10.1089/bio.2020.0032","url":null,"abstract":"<p><p>The human microbiome encompasses a variety of microorganisms that change dynamically and are in close contact with the body. The microbiome influences health and homeostasis, as well as the immune system, and any significant change in this equilibrium (dysbiosis) triggers both acute and chronic health conditions. Microbiome research has surged, in part, due to advanced sequencing technologies enabling rapid, accurate, and cost-effective identification of the microbiome. A major prerequisite for stool sample collection to study the gut microbiome in longitudinal prospective studies requires standardized protocols that can be easily replicated. However, there are still significant bottlenecks to stool specimen collection that contribute to low patient retention rates in microbiome studies. These barriers are further exacerbated in solid organ transplant recipients where diarrhea is estimated to occur in up to half the patient population. We sought to test two relatively easy sample collection methods (fecal swab and wipes) and compare them to the more cumbersome \"gold\" standard collection method (scoop) using two different sequencing technologies (16S ribosomal RNA sequencing and shotgun metagenomics). Our comparison of the collection methods shows that both the swabs and the wipes are comparable to the scoop method in terms of bacterial abundance and diversity. The swabs, however, were closer in representation to the scoop and were easier to collect and process compared to the wipes. Potential contamination of the swab and the wipe samples by abundant skin commensals was low in our analysis. Comparison of the two sequencing technologies showed that they were complementary, and that 16S sequencing provided enough coverage to detect and differentiate between bacterial species identified in the collected samples. Our pilot study demonstrates that alternative collection methods for stool sampling are a viable option in clinical applications, such as organ transplant studies. The use of these methods may result in better patient retention recruitment rates in serial microbiome studies.</p>","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"425-440"},"PeriodicalIF":1.6,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/bio.2020.0032","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38301133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNA and DNA Integrity Remain Stable in Frozen Tissue After Long-Term Storage at Cryogenic Temperatures: A Report from the Ontario Tumour Bank.","authors":"Rachel Kelly,&nbsp;Monique Albert,&nbsp;Manon de Ladurantaye,&nbsp;Melissa Moore,&nbsp;Olusola Dokun,&nbsp;John M S Bartlett","doi":"10.1089/bio.2018.0095","DOIUrl":"https://doi.org/10.1089/bio.2018.0095","url":null,"abstract":"<p><p><b><i>Background:</i></b> It is widely assumed that the integrity of tissue specimens remains stable indefinitely if preserved at cryogenic temperatures. With biobanking reaching a level of maturity where samples are increasingly stored for 10 years and beyond, this assumption of prolonged stability should be tested. Data from such an assessment are critical to verify if samples stored for extended durations remain \"fit for purpose\" or if there is need to reconsider the utility of samples stored beyond a certain timeframe. The Ontario Tumour Bank has been collecting samples since 2004, and assesses a random selection of frozen samples each year for RNA and DNA integrity as a part of ongoing quality control (QC) practices. This historical quality assessment data provide a unique opportunity to assess the impact of extended storage on nucleic acid integrity using replicate samples that remain in the bank in the present day as comparators. <b><i>Methods:</i></b> To examine the stability of fresh-frozen tumor tissue stored at cryogenic temperatures, RNA was extracted and analyzed from 87 cases over 14 disease sites stored long term in vapor-phase liquid nitrogen (LN2) (approximately -180°C). Historical QC data were compared against new data after re-extraction of replicate samples to determine the effect of extended storage on RNA quality. In addition, DNA was extracted from a subselection of samples (<i>n</i> = 20) to determine the effect of prolonged storage on DNA integrity. <b><i>Results:</i></b> No time-dependent decrease in tissue RNA or DNA quality, as measured by RNA integrity number (RIN) and DNA integrity number, was observed over an 11-year period. As a secondary observation, RNA integrity was not predictive of DNA integrity: DNA quality may still be very good, and as such RIN scores should not be used as a substitute indicator for evaluating DNA. <b><i>Conclusions:</i></b> Extended cryogenic storage beyond 2-11 years remains a viable option for maintaining the high quality of specimens in biobanks.</p>","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"282-287"},"PeriodicalIF":1.6,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/bio.2018.0095","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40447833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Extender and Freezing Rate on Quality Parameters and In Vitro Fertilization Capacity of Alpaca Spermatozoa Recovered from Cauda Epididymis.","authors":"Guiulfo Mamani-Mango,&nbsp;Milagros Moina Gonzales,&nbsp;Martín Ramos Hidalgo,&nbsp;José Mendoza Mallma,&nbsp;Jaime Ruiz Béjar,&nbsp;Victoria Rivas Palma,&nbsp;Edwin Mellisho Salas","doi":"10.1089/bio.2018.0021","DOIUrl":"https://doi.org/10.1089/bio.2018.0021","url":null,"abstract":"<p><p>In alpacas, improvement of reproductive efficiency of male camelids is limited by the small testicular size, low spermatozoa production, and low quality of semen. In this study we aim to evaluate the effect of two extenders and two freezing rates on post-thaw quality of sperm recovered from alpaca epididymis with two methods (flushing and mincing), and to evaluate the in vitro fertilization (IVF) capacity of frozen sperm selected with two different selection methods (washing and swim-up). Sperm samples were processed with Tris-egg yolk or Bioxcell^® extenders and frozen with slow freezing and fast freezing. The oocytes were coincubated with spermatozoa for 72 hours, and cleavage rates were recorded afterward. The results indicated that the recovery method did not influence sperm quality (∼70%). However, total sperm recovery was significantly lower for the flushing method than the mincing method. The sperm quality was influenced by the freezing extender (23.3% vs. 33.2%) and freezing rate (20.9% vs. 35.7%). When comparing different methods of sperm selection for IVF, no differences were observed on cleavage rate except for the fact that the concentration of sperm from swim-up method (20.6%) was significantly lower than the one obtained from the washing method (78.7%). The recovery technique of sperm does not affect sperm quality and the method of fast freezing was shown to be the most effective for cryopreservation of alpaca sperm.</p>","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"39-45"},"PeriodicalIF":1.6,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/bio.2018.0021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40535762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Nucleic Acid Quality Control Strategy for Frozen Tissues from a Biobank of High-Risk Pregnancy.","authors":"Hong Gao,&nbsp;Yanhong Liu,&nbsp;Jie Ding,&nbsp;Jun Yang,&nbsp;Biao Zhang,&nbsp;Yue Hu,&nbsp;Meiling Ge,&nbsp;Qing Ye","doi":"10.1089/bio.2018.0041","DOIUrl":"https://doi.org/10.1089/bio.2018.0041","url":null,"abstract":"<p><strong>Background: </strong>The preservation of placental and fetal tissues will contribute to studying the pathogenesis of high-risk pregnancy diseases. However, few studies have focused on the effects of different preservation methods and cold ischemia time (CIT) on the quality of nucleic acids. An available quality control (QC) strategy will be beneficial to evaluate these effects for high-risk pregnancy biobanks.</p><p><strong>Methods: </strong>We established an evaluation strategy of nucleic acid QC by analyzing total RNA and genomic DNA (gDNA). Through this strategy, the effects of CIT, cryoprotectants (CPAs), and freeze/thaw cycles on the yield and integrity of placental RNA were analyzed. In addition, the effects of CIT on the yield and integrity of fetal DNA were determined.</p><p><strong>Results: </strong>For placental samples, there was no significant difference in RNA integrity (CIT <2 hours). After several freeze/thaw cycles, the RNA quality number values of placental samples in the CPA-free group and in the RNasin (TRIzol) group were decreased. For fetal samples, the DNA integrity of different organs (CIT <24 hours) was completely satisfactory, but it declined with the extension of CIT. Furthermore, different organs had different tolerances to cold ischemia, and the rank was as follows: skin, heart, liver, and placenta. In addition, the content of medium-length (600 bp) and long (1310 bp) fragments of gDNA were mainly reduced with the extension of CIT.</p><p><strong>Conclusion: </strong>The RNA integrity of placental tissue was affected by CIT significantly. It is recommended that placenta should be cryopreserved within 2 hours (4°C) from isolation. To ensure DNA quality of fetal tissues, the samples are suggested to be frozen within 24 hours (4°C) from isolation. On the contrary, if samples have a long CIT, skin is superior to other organs in the aspect of biobanking donor's genetic information.</p>","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"18-26"},"PeriodicalIF":1.6,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/bio.2018.0041","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40444522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Pectins on Water Crystallization Pattern and Integrity of Cells During Freezing.","authors":"Andrey Nikolayevich Khudyakov,&nbsp;Larisa Georgievna Kuleshova,&nbsp;Oksana Olegovna Zaitseva,&nbsp;Marta Igorevna Sergushkina,&nbsp;Konstantin Aleksandrovich Vetoshkin,&nbsp;Tatyana Vitalyevna Polezhaeva","doi":"10.1089/bio.2018.0066","DOIUrl":"https://doi.org/10.1089/bio.2018.0066","url":null,"abstract":"The ability of various pectin polysaccharides to modify the morphological structure of ice during the phase transitions from water to ice was studied. Pectins were isolated from Sosnowsky's hogweed Heracleum sosnowskyi Manden (heracleuman-N6HS), tansy Tanacetum vulgare L. (tanacetan-N7TVF), and Rauwolfia serpentina Benth callus (rauwolfian-N8RS). Pectins were isolated by multistep extraction. The effect of pectins was assessed using osmometry, thermographic analysis, and cryomicroscopy. A concentrate of leukocytes was used as the sample for the subsequent freezing step. The condition of the leukocyte membrane, and lysosomal and phagocytic activity after a freezing-warming process were assessed. Osmotic concentrations of the pectin polysaccharide solutions were found to be very low. The 0.4 wt % N7TVF solution had the highest osmotic concentration as well as freezing point; however, the duration of its crystallization plateau was lower than that of the 0.4 wt % and 0.2 wt % N6HS solutions. All studied polysaccharide solutions demonstrated a high linear rate of ice crystal growth. There were statistically significant differences between the melting rates for the 0.2% solutions of the pectins, N6HS and N7TVF, N6HS and N8RS, as well as between concentrations for the pectin N7TVF and between concentrations for the pectin N8RS. The data on the integrity of cells that are frozen in a medium containing polysaccharides may indicate a cryoprotective effect of the N7TVF and N8RS pectins, that is, tanacetan from tansy and rauwolfian from rauwolfia. The most effective modifier among the substances, which were studied by us, was the N7TVF pectin polysaccharide (tanacetan from tansy).","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"52-57"},"PeriodicalIF":1.6,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/bio.2018.0066","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40445012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Cellular Lipids on Cryopreservation of Mammalian Oocytes and Preimplantation Embryos: A Review.","authors":"Sergei Amstislavsky,&nbsp;Valentina Mokrousova,&nbsp;Eugeny Brusentsev,&nbsp;Konstantin Okotrub,&nbsp;Pierre Comizzoli","doi":"10.1089/bio.2018.0039","DOIUrl":"https://doi.org/10.1089/bio.2018.0039","url":null,"abstract":"<p><p>Lipids are among the most abundant and essential cell components. Specifically, cytoplasmic lipid droplets (LDs) play crucial roles in cellular energy homeostasis. The foci of this review are (1) the composition and roles of lipids during oocyte maturation and early embryonic development, (2) possible causes of cryoinjuries in lipid-rich oocytes/embryos, and (3) ways to overcome these detrimental effects. Recent reports show that LDs in oocytes and embryos are not only energy depots but also are active organelles, possessing many other functions. In addition, analysis of the current literature confirms that lipid phase transition followed by phase separation during cryopreservation is one of the major causes of cryodamage in lipid-rich oocytes and embryos. While LDs and cell membranes are sensitive to chilling and freezing conditions, recent advances in vitrification and delipidation of lipid-rich oocytes and embryos partly mitigate cryodamage. The multidisciplinary approach is critical to reveal mechanisms underlying cryodamage and provides a theoretical basis for optimal cryopreservation of lipid-rich oocytes/embryos.</p>","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"76-83"},"PeriodicalIF":1.6,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/bio.2018.0039","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40535783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality Control System in an Obstetrics and Gynecology Disease Biobank.","authors":"Yanhong Liu,&nbsp;Hong Gao,&nbsp;Yue Hu,&nbsp;Jie Ding,&nbsp;Meiling Ge,&nbsp;Qing Ye","doi":"10.1089/bio.2018.0056","DOIUrl":"https://doi.org/10.1089/bio.2018.0056","url":null,"abstract":"<p><strong>Aim: </strong>To ensure that sample quality meets the requirements of experimental research, the gynecology and obstetrics biobank of the Nanjing Drum Tower hospital designed different quality control methods for relevant types of samples. A range of quality control procedures has been formulated.</p><p><strong>Methods: </strong>The sample types were frozen tissue, paraffin-embedded tissue, optimal cutting temperature (OCT)-embedded tissue, plasma, buffy coat, serum, blood clots, and urine. Different categories of samples from a random selection of 1% of cases were analyzed for quality control experiments: (i) frozen tissue, buffy coat, and blood clots: RNA and DNA were extracted and the concentration, purity, and integrity were determined; (ii) paraffin-embedded tissue: morphological observations were made after hematoxylin-eosin staining and immunohistochemical detection of β-actin or CD10; (iii) OCT-embedded tissue: hematoxylin-eosin staining and immunofluorescence detection of β-actin; and (iv) frozen tissue samples derived from different organs of 18 fetal autopsy specimens with different cold ischemia times (CITs), 0-12 hours, 12-18 hours, 18-24 hours, and 24-48 hours, were chosen to study RNA quality. There is no universally recognized quality control index for plasma, serum, and urine, so the quality of samples was evaluated from feedback from the research projects in which the samples were used.</p><p><strong>Results: </strong>Currently, there are ∼2000 cases and 360,000 sample vials in the biobank. According to the experiments, (i) the concentration and purity of all nucleic acids of selected samples were qualified; (ii) for frozen tissues with a CIT ≤1 hour, using a qualified standard RNA quality number (RQN) ≥7, the qualification rate was 90%; (iii) frozen tissues with CIT between 1 and 18 hours, using a qualified standard RQN ≥5, the qualification rate was 61.1%; (iv) all of the paraffin-embedded tissues qualified for morphological observation; (v) the qualification rate of OCT-embedded tissue was 89%; and (vi) CIT had a great influence on the integrity of frozen tissue RNA. As the tissue CIT lengthened, the integrity of the RNA decreased. The RNA integrity parameters of different tissue types in the same specimen were significantly different.</p><p><strong>Conclusions: </strong>A quality control system was constructed in an obstetrics and gynecology disease biobank with various types of diseases and abundant samples. Using specific quality control experiments for different types of samples was a reliable operating strategy that can be beneficial for providing qualified research resources. For birth defect autopsy specimens, the samples used for RNA research should have a CIT of at least <12 hours.</p>","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"27-38"},"PeriodicalIF":1.6,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/bio.2018.0056","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40544605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effect of Sucrose and Antioxidants on Cryopreservation of Sperm Motility and DNA Integrity in C57BL/6 Mice.","authors":"Xiaowei Shi,&nbsp;Huanhuan Hu,&nbsp;Guojie Ji,&nbsp;Jing Zhang,&nbsp;Rui Liu,&nbsp;Han Zhang,&nbsp;Mingwen Li","doi":"10.1089/bio.2018.0037","DOIUrl":"https://doi.org/10.1089/bio.2018.0037","url":null,"abstract":"<p><p>Currently the most popular mouse sperm freezing medium is R18SM3+MTG containing 18% raffinose, 3% skim milk, and 0.5 mM monothioglycerol (MTG), but there is no information available about whether MTG and other antioxidants can cryoprotect mouse sperm DNA integrity. It is also uncertain if sucrose can be used successfully for sperm cryopreservation. In this report we compared the cryoprotective effects of sucrose and raffinose, as well as the antioxidants MTG, reduced glutathione (GSH), and quercetin on sperm motility, DNA integrity, and fertility in the C57BL/6J mouse strain. Results show that: (1) 10% sucrose in the presence of 3% skim milk and 0.5 mM MTG (S10SM3+MTG) was as effective as R18SM3+MTG (<i>p</i> > 0.05) in cryopreserving sperm motility (21.0% ± 4.0% vs. 19.0% ± 3.6%), DNA integrity (8.1% ± 1.5% vs. 9.0% ± 1.5% TUNEL positive), fertilization rate (48.3% ± 7.5% vs. 45.0% ± 7.9%), and pup birth rate (36.7% ± 10.0% vs. 37.7% ± 3.5%); (2) Supplementation of freezing medium with MTG (0.5 mM), GSH (0.5 mM), or quercetin (25 μM) had a significant (<i>p</i> < 0.05) cryoprotective effect on sperm motility recovery rate compared to that of controls (MTG, 32.7% ± 10.7% vs. 19.4% ± 4.2%; GSH, 34.3% ± 3.7% vs. 24.5% ± 1.8%; quercetin, 36.3% ± 3.3% vs. 25.1% ± 3.6%); and (3) Cryopreservation significantly increased sperm DNA fragmentation level (16.4% ± 2.3% in R18SM3 and 14.6% ± 2.5% in S10SM3) compared to that of fresh sperm (3.0% ± 2.0%); however, supplementation with MTG (0.5 mM), GSH (0.5 mM), or quercetin (25 μM) significantly (<i>p</i> < 0.05) decreased the sperm DNA fragmentation level (MTG, 8.1% ± 1.5%; GSH, 9.0% ± 1.5%; and quercetin, 8.3% ± 1.3%). It was concluded that sucrose can be used as effectively as raffinose for mouse sperm cryopreservation, and supplementation of MTG, GSH, or quercetin at an appropriate concentration can help cryoprotect sperm motility and DNA integrity.</p>","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"444-450"},"PeriodicalIF":1.6,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/bio.2018.0037","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40442893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Barriers and Opportunities in Consent and Access Procedures in Low- and Middle-Income Country Biobanks: Meeting Notes from the BCNet Training and General Assembly.","authors":"Ma'n H Zawati, Anne Marie Tassé, Maimuna Mendy, Elodie Caboux, Michael Lang","doi":"10.1089/bio.2017.0081","DOIUrl":"10.1089/bio.2017.0081","url":null,"abstract":"<p><p>As biobanking research in low- and middle-income countries (LMICs) continues to grow, novel legal and policy considerations have arisen. Also, while an expansive literature has developed around these issues, the views and concerns of individual researchers in these contexts have been less actively studied. These meeting notes aim to contribute to the growing literature on biobanking in LMICs by communicating a number of challenges and opportunities identified by biobank researchers themselves. Specifically, we describe concerns that emerge in consent and access policy domains. First, we present a review of the literature on distinct policy and legal concerns faced in LMICs, giving special attention to the general absence of practitioner perspectives. From there, we outline and discuss considerations that were raised by meeting participants at a Biobank and Cohort Building Network (BCNet) Ethical, Legal, and Social Issues training program. We conclude by proposing that the unique perspectives of biobank researchers in LMICs should be given serious attention and further research on these perspectives should be conducted.</p>","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":"16 3","pages":"171-178"},"PeriodicalIF":1.2,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39985772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Need for Research Infrastructures: A Narrative Review of Large-Scale Research Infrastructures in Biobanking.","authors":"Anthony Larsson","doi":"10.1089/bio.2016.0103","DOIUrl":"https://doi.org/10.1089/bio.2016.0103","url":null,"abstract":"<p><strong>Background: </strong>Distributed Research Infrastructures are gaining political traction in Europe to facilitate scientific research. This development has gained particular momentum in the area of biobanking where cross-national attempts have been made toward harmonizing the biobanking standards across the European Union through the establishment of the organization BBMRI (BioBanking and Biomolecular Resources Research Infrastructure). BBMRI exists as separate national nodes across several European countries, although Sweden took on a pioneering role in its early stages. Thus, the Swedish node, BBMRI.se, was set up in 2009.</p><p><strong>Purpose: </strong>To document publications addressing the current debate on large-scale distributed medical and/or biobank Research Infrastructures and identify the most pressing issues discussed by these articles through a narrative review.</p><p><strong>Methods: </strong>The Web of Science (WOS) and PubMed databases were searched to find prior studies of large-scale medical Research Infrastructures, with no limits set with regard to study design and/or time period. All identified articles published up until March 2016 were included in the initial review.</p><p><strong>Results: </strong>A total of 145 articles were retrieved from WOS and PubMed, though merely 17 ultimately made it past the final exclusion criteria. About two-thirds of the articles listed a first author affiliated to a European country. The articles most commonly discussed the need for developing and expanding the use of \"infrastructures.\"</p><p><strong>Practical implications: </strong>The future of scientific research will call for a deeper and more widespread multidisciplinary collaboration. This will emphasize the need of research seeking to optimize the preconditions of securing sustainable scientific collaboration. Future investigators will thus need to understand the components and mechanisms of Research Infrastructures in addition to acquiring knowledge of how to build, manage, brand, and promote them as well.</p>","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":"15 4","pages":"375-383"},"PeriodicalIF":1.6,"publicationDate":"2017-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/bio.2016.0103","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39983323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}