Biopreservation and Biobanking最新文献

{"title":"Influence of Microwave-Assisted Drying on Structural Integrity and Viability of Testicular Tissues from Adult and Prepubertal Domestic Cats.","authors":"Herlon Victor Rodrigues Silva,&nbsp;Andréia Maria da Silva,&nbsp;Pei-Chih Lee,&nbsp;Bruna Farias Brito,&nbsp;Alexandre Rodrigues Silva,&nbsp;Lúcia Daniel Machado da Silva,&nbsp;Pierre Comizzoli","doi":"10.1089/bio.2020.0048","DOIUrl":"https://doi.org/10.1089/bio.2020.0048","url":null,"abstract":"<p><p>Anhydrous preservation is a promising approach for storage of living biomaterials at nonfreezing temperatures. Using the domestic cat model, the objectives of this study were to characterize changes in histology, DNA integrity, and viability of testicular tissues from adult versus prepubertal individuals during microwave-assisted drying. Testes from each age group were cut into small pieces before reversible membrane permeabilization, exposure to trehalose, and microwave-assisted drying during different time periods. In Experiment 1, water content was monitored for up to 40 minutes of drying. Tissues from adult or prepubertal cats experienced similar decreases of water content during the first 10 minutes. Desiccation progressed slowly between 10 and 20 minutes and then remained stable. In Experiment 2, structural properties were explored at 5, 10, and 20 minutes of desiccation. Percentages of normal seminiferous tubules were lower after 20 minutes drying in adult (43%) than in prepubertal tissues (61%). At the same time point, the proportion of cell degeneration was higher in adult (53%) than prepubertal tissues (28%). Percentages of intact DNA in tissues remained above 85% regardless of the microwave time in both age groups. Lastly, adult and prepubertal tissues only lost 33% of viability in both age groups. Collective results demonstrated for the first time that normal morphology, incidence of degeneration, DNA integrity, and viability of testicular tissues remained at acceptable levels during microwave-assisted drying for 20 minutes. Overall, prepubertal testicular tissues appeared to be more resilient to microwave-assisted desiccations than adult tissues. Importantly, water loss in the presence of trehalose after 20 minutes of desiccation already is compatible with long-term storage of testicular tissues at temperatures above -20°C, which is one step closer to future storage at supra-zero temperatures.</p>","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"415-424"},"PeriodicalIF":1.6,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/bio.2020.0048","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38260193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Birth of a Giant Panda Cub After Artificial Insemination with Frozen-Thawed Semen: A Powerful Reminder About the Key Role of Biopreservation and Biobanking for Wildlife Conservation.","authors":"Pierre Comizzoli","doi":"10.1089/bio.2020.29076.pjc","DOIUrl":"https://doi.org/10.1089/bio.2020.29076.pjc","url":null,"abstract":"","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"349-350"},"PeriodicalIF":1.6,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/bio.2020.29076.pjc","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38387543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Testing the Stability of Plasma Protein and Whole Blood RNA in Archived Blood of Loggerhead Sea Turtles, <i>Caretta caretta</i>.","authors":"Kelly Townsend,&nbsp;Jennifer Ness,&nbsp;Jennifer Hoguet,&nbsp;Nicole I Stacy,&nbsp;Lisa M Komoroske,&nbsp;Jennifer M Lynch","doi":"10.1089/bio.2020.0026","DOIUrl":"https://doi.org/10.1089/bio.2020.0026","url":null,"abstract":"<p><p>Sample storage conditions can affect accuracy and reproducibility of biological measurements. Storing samples rapidly at the lowest available temperatures is considered ideal but is not always feasible when sampling in remote and logistically challenging field conditions, as is often the case with sea turtles. The objective of this study was to examine the stability of plasma proteins and quality of whole blood RNA from loggerhead sea turtle samples collected as part of an eighteen-year-long curated specimen collection. These biological variables are often used to assess sea turtle health; therefore, it is necessary to maintain the integrity of these components during storage. Protein electrophoresis was conducted on heparinized plasma from individual turtles collected in 2018 (<i>n</i> = 3), 2008 (<i>n</i> = 3), and 2001 (<i>n</i> = 3). Plasma was also pooled from four turtles sampled in 2018 and subjected to various storage temperatures. Whole blood was collected in blood collection tubes containing sodium heparin or PAXgene tubes with an RNA preservative. These were subjected to different storage treatments that can possibly occur during logistically difficult field sampling. Following various treatments, plasma proteins showed minor differences across collection years and no differences among storage treatments were observed, even when exposed to 38°C for three hours. RNA quality was assessed from whole blood using an RNA integrity number (RIN). RINs were poor from sodium heparin tubes that were frozen and from PAXgene tubes after an extended thaw. High-quality RNA was obtained from sodium heparin tubes that were never frozen and from PAXgene tubes with freezing delayed by up to 11 days. Overall, these results indicate that plasma proteins remain stable over time and when exposed to undesirable storage conditions, and RNA degrades rapidly in sea turtle blood after freezing and when not properly preserved. These aspects are important to consider when planning sampling protocols and logistics for optimal long-term sample preservation.</p>","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"358-366"},"PeriodicalIF":1.6,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/bio.2020.0026","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38093598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Synergism Between Carboxylated Poly-l-Lysine and Glycerol on Freezability of Nili-Ravi Buffalo (<i>Bubalus bubalis</i>) Semen.","authors":"Shamim Akhter,&nbsp;Muhammad Amjad Awan,&nbsp;Javeria Arshad,&nbsp;Bushra Allah Rakha,&nbsp;Muhammad Sajjad Ansari,&nbsp;Sajid Iqbal","doi":"10.1089/bio.2019.0120","DOIUrl":"https://doi.org/10.1089/bio.2019.0120","url":null,"abstract":"<p><p>Carboxylated poly-l-lysine (CPLL), an ampholytic polymer, has remarkable cryoprotective properties. It was hypothesized that CPLL will reduce/replace glycerol in extender and improve the freezability of buffalo semen. The objective was to evaluate various combinations of CPLL and glycerol in extenders for any synergism toward cryosurvivability of Nili-Ravi buffalo sperm. Semen collected from four Nili-Ravi buffalo bulls (ejaculates = 40; replicates = 5) was diluted in tris-citric acid based extenders, Control: (0% CPLL +7% Glycerol); E1: (1% CPLL +6% Glycerol); E2: (2% CPLL +5% Glycerol); E3: (3% CPLL +4% Glycerol); E4: (4% CPLL +3% Glycerol); E5: (5% CPLL +2% Glycerol); E6: (6% CPLL +1% Glycerol), and E7: (7% CPLL +0% Glycerol), and cryopreserved using a programmable cell freezer. Percentages of post-thaw sperm motility, plasma membrane integrity, and acrosomal integrity were found to be higher (<i>p</i> < 0.05) in extenders E1, E2, E3, E4, and E5 compared to E6 and the control. Sperm livability (%; live/dead ratio) and viability (%; live sperm with intact acrosome) were higher (<i>p</i> < 0.05) in extender E4 compared to all the other extenders. Sperm DNA integrity was higher (<i>p</i> < 0.05) in extender E2, E3, E4, and E5 compared to control, E1, and E6 extenders. Sperm lipid peroxidation levels were lower (<i>p</i> < 0.05) in E3, E4, E5, and E6 compared to control, E1, E2, and E7 extenders. Total antioxidant capacity of seminal plasma was higher (<i>p</i> < 0.05) in extenders E5, E6, and E7 than control, E1, E2, E3, and E4 extenders. It is concluded that synergism between CPLL and glycerol (4% CPPL and 3% Glycerol) seems to improve the freezability of Nili-Ravi buffalo semen by reducing oxidative stress.</p>","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"367-375"},"PeriodicalIF":1.6,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/bio.2019.0120","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38105405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Returning Results of Stored Biological Samples and Biobanks: Perspectives of Saudi Arabian Biomedical Researchers.","authors":"Ghiath Alahmad,&nbsp;Haneen Alzahrany,&nbsp;Adel F Almutairi","doi":"10.1089/bio.2020.0002","DOIUrl":"https://doi.org/10.1089/bio.2020.0002","url":null,"abstract":"<p><p>Scientific medical research involving human samples often leads to improved diagnosis, the discovery of treatment modalities, or the identification of possible risk factors for many diseases. Some findings, including incidental findings, may be important to donors, and some may require intervention. This study aimed to explore the perspectives of health care professionals in their use of stored biological samples for biomedical research regarding the concept of the research results and the challenges of informing donors regarding the results. This qualitative study involved 19 medical researchers doing research with stored biological samples and biobanks. The data were gathered during face-to-face interviews in English using a semistructured interview technique. The participants provided rich and illuminating experiences, framed in the following themes: the professional duty of researchers to return the research results and the right of donors to know; factors affecting informing donors of results (e.g., severity of disease; impact of the provided information; reliability of the research results; and donor approval); challenges to physically returning the results; and the nature of the informed consent, as well as the elements required in the informed consent documentation. Although the majority of researchers agree on the importance of returning research results, some have contradictory views such as that returning research results is not the researcher's responsibility. The study results also support the view that a number of elements should be included in the informed consent, such as the intention of informing the donors of the results as well as the benefits and risks.</p>","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"395-402"},"PeriodicalIF":1.6,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/bio.2020.0002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38187419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Age and Type of Media on Growth Kinetics of Human Amniotic Fluid Stem Cells.","authors":"Bahareh Borzou,&nbsp;Davood Mehrabani,&nbsp;Shahrokh Zare,&nbsp;Marzieh Zamani-Pereshkaft,&nbsp;Jason P Acker","doi":"10.1089/bio.2019.0103","DOIUrl":"https://doi.org/10.1089/bio.2019.0103","url":null,"abstract":"<p><p><b><i>Aim:</i></b> This study compared growth kinetics of human amniotic fluid stem cells (hAFSCs) in different maternal age groups and two different media of AmnioMAX and Dulbecco's modified Eagle's medium (DMEM). <b><i>Materials and Methods:</i></b> Three milliliters of amniotic fluid (AF) was provided from 16 pregnant women who were referred for amniocentesis from 16 to 18 weeks of gestation. Mothers were divided to 20-29 (<i>n</i> = 5), 30-39 (<i>n</i> = 5) and 40-49 (<i>n</i> = 6) years old age groups. AF was immediately centrifuged and the cell pellet was cultured. Cells were characterized morphologically, by flow cytometry and for osteogenic and adipogenic inductions. Population doubling time (PDT) and growth kinetics were determined. AFSCs cultured in AmnioMAX were compared in various age groups. A comparison of growth kinetics of AFSCs cultured in AmnioMAX and DMEM from 40 to 49 years old pregnant women was undertaken. <b><i>Results:</i></b> AFSCs were adherent to culture flasks and were spindle shape, and positive for osteogenic and adipogenic inductions and for expression of CD73, CD90 and CD105 markers, but negative for CD34 and CD45. PDT among 20-29, 30-39, and 40-49 years old women was 30.9, 38.3, and 43.9 hours, respectively showing a higher cell proliferation in younger ages. When comparing AmnioMAX and DMEM, PDT was 53 and 96.9 hours, respectively denoting to a higher cell proliferation in AmnioMAX. <b><i>Conclusions:</i></b> Higher proliferation and plasticity of hAFSCs were noted in AmnioMAX and in younger mothers' samples. These findings can be added to the literature and open a new avenue in regenerative medicine, when hAFSCs are targeted for cell therapy purposes.</p>","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"389-394"},"PeriodicalIF":1.6,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/bio.2019.0103","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38267085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Testing a Novel Disposable Aqueous Humor Collector: An Approach to Improve Safety, Accuracy, and Efficiency.","authors":"Yi Qu,&nbsp;Youxin Chen,&nbsp;Chan Zhao","doi":"10.1089/bio.2020.0039","DOIUrl":"https://doi.org/10.1089/bio.2020.0039","url":null,"abstract":"<p><p>Aqueous humor (AH) is a useful biofluid for differential diagnosis and disease monitoring in a variety of ophthalmologic conditions. There are no commercially available medical devices specially designed for AH sampling. To improve the safety, accuracy, efficiency, and convenience of anterior chamber (AC) paracentesis, a novel vacuum-based disposable AH collector with a draw volume of 50 μL was designed. The safety and performance of this novel medical device was tested in New Zealand white rabbits. A commonly used 25G 1 mL syringe was used as the control device with target AH collection volume also intentionally set at 50 μL. The 36 eyes included in the study (18 rabbits) were divided into collector (R), collector (L), syringe (R), and syringe (L) subgroups, and each included 9 eyes (R/L indicates the paracentesis was performed by right/left hand). The mean AH volume collected by the collector (R), collector (L), syringe (R), and syringe (L) subgroups were 46.66 ± 3.37 (range: 39.20-50.40), 48.71 ± 2.88 (range: 45.00-53.60), 85.11 ± 18.70 (range: 64.00-123.50), and 80.68 ± 20.87 (range: 36.8-115.8) μL, respectively. The mean absolute deviation from the target volume and mean operation time of the collector subgroups were significantly lower than the syringe subgroups. Seidel tests revealed no AH leakage in any of the tested eyes. This study revealed that this novel AH collector facilitates one-handed AC paracentesis and accurate AH sampling, and appeared to be safer and more efficient than the traditional syringe-based techniques.</p>","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"449-453"},"PeriodicalIF":1.6,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/bio.2020.0039","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38273644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison Between Three Cryoprotectants in the Freezing of <i>Mazama americana</i> Semen Collected by Artificial Vagina.","authors":"Maria C L Alvarez,&nbsp;Luciana D Rola,&nbsp;José M B Duarte","doi":"10.1089/bio.2020.0012","DOIUrl":"https://doi.org/10.1089/bio.2020.0012","url":null,"abstract":"<p><p>Maintaining genetic variability is an important part of the conservation of endangered species, so the construction of germplasm banks is essential. Several species of the genus <i>Mazama</i> endure constant pressure in their natural habitat and are threatened with extinction. The correct manipulation and adequacy of the diluents and cryoprotectants must be studied to be successful in the formation of these banks. The purpose of this study was to evaluate the efficiency of three different cryoprotectants in sperm cryopreservation in the species <i>Mazama americana</i>: 6% glycerol (GLY), 3% ethylene glycol (ETG), and 5% dimethylformamide (DMF). Semen was obtained with the lateral deviation of the penis to an artificial vagina. In the pre-freeze and post-thaw periods, motility, vigor, membrane integrity, acrosome integrity, and sperm cell morphology were evaluated for each of the cryoprotectants. Post-thaw motility was higher when semen was frozen with cryoprotectants GLY and DMF (55.31 ± 7.39 and 55.94 ± 2.77, respectively), compared with the result obtained for ETG (48.13 ± 2.39). For major defects (MaD), a difference was observed between the pre- and post-cryopreservation periods, such that DMF generated a higher number of post-thaw MaD (25.94 ± 5.37). All cryoprotectants were efficient for cryopreservation of <i>M. americana</i> semen, resulting in samples with satisfactory viability after thawing. However, the medium with the cryoprotectants GLY, at a concentration of 6%, and DMF, at a concentration of 5%, were preferable.</p>","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"351-357"},"PeriodicalIF":1.6,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/bio.2020.0012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38110852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of CryoMACS Freezing Bags with Maco Biotech Freezing-Ethinyl Vinyl Acetate Bags for Hematopoietic Progenitor Cells Cryopreservation Using a CD34⁺-Enriched Product.","authors":"Valentina Becherucci,&nbsp;Silvia Bisin,&nbsp;Stefano Ermini,&nbsp;Luisa Piccini,&nbsp;Valentina Gori,&nbsp;Francesca Gentile,&nbsp;Riccardo Ceccantini,&nbsp;Elena De Rienzo,&nbsp;Barbara Bindi,&nbsp;Paola Pavan,&nbsp;Vanessa Cunial,&nbsp;Elisa Allegro,&nbsp;Francesca Brugnolo,&nbsp;Franco Bambi","doi":"10.1089/bio.2019.0135","DOIUrl":"https://doi.org/10.1089/bio.2019.0135","url":null,"abstract":"<p><p><b><i>Background:</i></b> Hematopoietic progenitor cells (HPCs) cryopreservation have applications, especially in the autologous setting, allowing therapeutic use several years after collection. Cryopreservation aims to preserve the therapeutic properties of HPCs, and successful cryopreservation depends on several factors such as preservation procedures, biopreservation media, freezing rates, and thawing procedures. In this context, the choice of the freezing bag is critical as it provides mechanical protection during the freezing process. Since Maco Biotech Freezing-ethinyl vinyl acetate (EVA) Bags^® are no longer available in our country, a comparative study was developed to verify bioequivalence with the Miltenyi CryoMACS^® freezing bag. <b><i>Methods:</i></b> In this study, a CD34⁺-enriched product was used to better reproduce HPC apheresis. Freezing bags were filled with the same volume, cryopreserved with controlled rate freezing, and stored in the vapor phase of liquid nitrogen for at least 6 months. After thawing, all bags were tested for integrity and sterility using a microbial challenge. In addition, a comparison was developed by evaluating recovery of white blood cells, mononuclear cells, lymphocytes, and CD34⁺ cells. <b><i>Results:</i></b> No significant differences between the two manufacturers' bags have been observed in terms of the evaluated parameters. Data were confirmed, even comparing bags according to filling volume. Data presented in this study support the conclusion that CryoMACS freezing bags are bioequivalent to Maco Biotech Freezing-EVA Bags for HPC cryopreservation.</p>","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"454-461"},"PeriodicalIF":1.6,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/bio.2019.0135","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38278217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of L-Carnitine on Equine Semen Quality During Liquid Storage.","authors":"Igor H A V Nery,&nbsp;Robespierre A J Araújo Silva,&nbsp;Helder M Souza,&nbsp;Lúcia C P Arruda,&nbsp;Millena M Monteiro,&nbsp;Desirée C M Seal,&nbsp;Girliane R Silva,&nbsp;Tânia M S Silva,&nbsp;Gustavo F Carneiro,&nbsp;André M Batista,&nbsp;Diogo R Câmara,&nbsp;Maria Madalena Pessoa Guerra","doi":"10.1089/bio.2020.0025","DOIUrl":"https://doi.org/10.1089/bio.2020.0025","url":null,"abstract":"<p><p>l-Carnitine (LC) plays a key role in sperm metabolism, easily providing energy through β-oxidation, which positively affects motility. The objective of this study was to investigate the association between blood plasma and seminal plasma LC levels, as well as the effect of LC as an additive in a skimmed milk-based extender during sperm storage at 5°C. In the first experiment, semen and blood samples from 14 Quarter Horse stallions were used. The LC content in blood plasma and seminal plasma was determined by spectrophotometry and their relationships with seminal parameters were evaluated. In the second experiment, ejaculates (<i>n</i> = 16) from four Quarter Horses were used. Each ejaculate was split into four treatment groups with different LC concentrations: 0 (control), 0.5, 1.0, and 2.0 mM. Sperm motility, integrity of plasma and acrosomal membranes, intracellular reactive oxygen species content, and plasma membrane stability were evaluated immediately after samples reached 5°C (0 hour) and after 24, 48, and 72 hours. There was a positive correlation (<i>p</i> < 0.05) between LC levels in seminal plasma with both sperm concentration and plasma and acrosomal membrane integrity. Furthermore, the addition of LC (1 and 2 mM) preserved the motility of equine sperm stored at 5°C. It was concluded that the concentrations of LC with seminal plasma present correlate to semen parameters and the addition of LC to skimmed milk-based extender preserves the motility of equine sperm stored at 5°C for up to 48 hours.</p>","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"403-408"},"PeriodicalIF":1.6,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/bio.2020.0025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38267086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}