Biopreservation and Biobanking最新文献

{"title":"Gene Expression Alteration of Sperm-Associated Antigens in Human Cryopreserved Sperm.","authors":"Samaneh Faraji,&nbsp;Leila Rashki Ghaleno,&nbsp;Mohsen Sharafi,&nbsp;Maryam Hezavehei,&nbsp;Mehdi Totonchi,&nbsp;Abdolhossein Shahverdi,&nbsp;Rouhollah Fathi","doi":"10.1089/bio.2020.0165","DOIUrl":"https://doi.org/10.1089/bio.2020.0165","url":null,"abstract":"<p><p><b><i>Background:</i></b> Sperm-associated antigens (SPAGs) are 18 types of proteins, some of which play important roles in various biological functions associated with assisted reproductive technology outcomes, and are consequently important to the success of fertility programs. Despite the favorable outcomes of fecundity rates among male patients with cancer using cryopreserved sperm, the detrimental impact of freezing on cells has been noted in many studies. Cryopreservation has been thought to have adverse effects on sperm quality through disruptions in the expressions of <i>SPAG</i> genes. This study aimed to evaluate the effects of cryopreservation on the expressions of <i>SPAGs</i> genes and their transcriptome alterations in human sperm. <b><i>Materials and Methods:</i></b> A total of 12 normal ejaculations were prepared using the density gradient centrifugation procedure, and the motile sperm fractions were divided into fresh and frozen groups. In the latter, sperm samples were mixed with SpermFreeze^® solution as the cryoprotectant. The cryovial of sperm suspension was first held just over nitrogen vapor and then dipped inside liquid nitrogen. After 3 days, the specimens were thawed in tap water and incubated for 2 hours for recovery. Then, RNA from sperm was extracted for <i>SPAG</i> gene expression analysis, using real-time polymerase chain reaction. <b><i>Results:</i></b> Our findings showed a decrease in expression of <i>SPAG5</i> (<i>p</i>-value = 0.009), <i>SPAG7</i> (<i>p</i>-value = 0.004), and <i>SPAG12 (SNU13/NHP2L1</i>; <i>p</i>-value = 0.039) genes during cryopreservation. <b><i>Discussion:</i></b> The results indicate that the freezing procedure could negatively affect gene expression and to some extent proteins in human spermatozoa. <b><i>Conclusion:</i></b> The alteration of <i>SPAG</i> expression could provide new information on the molecular correlation between cryopreservation and increased failure in intracytoplasmic sperm injection and <i>in vitro</i> fertilization.</p>","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"503-510"},"PeriodicalIF":1.6,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38915264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sculpting the Future of Biobanking Base by Base.","authors":"Melissa W VonDran,&nbsp;Bill Leinweber,&nbsp;Thomas J Bell","doi":"10.1089/bio.2021.29089.mwv","DOIUrl":"https://doi.org/10.1089/bio.2021.29089.mwv","url":null,"abstract":"","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"467-469"},"PeriodicalIF":1.6,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353984/pdf/bio.2021.29089.mwv.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39853135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunolocalization of Fertilin β, IZUMO1, and P34H in Ram Spermatozoa.","authors":"Gul Ipek Gundogan,&nbsp;Abit Aktas","doi":"10.1089/bio.2021.0006","DOIUrl":"https://doi.org/10.1089/bio.2021.0006","url":null,"abstract":"<p><p>According to various reports, current methods of sperm freezing destroy the integrity of the sperm plasma membrane and acrosome. This study aimed to determine the changes in the existence and location of three proteins, namely fertilin β, IZUMO1, and P34H, in ram spermatozoa. By using frozen-thawed spermatozoa, ejaculated fresh spermatozoa, and testicular and epididymal spermatozoa (obtained from caput, corpus, and caudal regions), the localizations of the mentioned proteins were performed using signal labeling with indirect immunofluorescence, and the quantification of these proteins was compared using Western blot analyses. Moreover, protein localization and signal labeling in fresh and frozen-thawed spermatozoa subjected to <i>in vitro</i> capacitation and acrosome reaction were compared. Using chlortetracycline (CTC) staining, as expected, it was detected that after incubating for 4 hours under capacitating conditions related to the control sample (0 hour), capacitated and acrosome-reacted sperm were increased (<i>p</i> < 0.001). Frozen-thawed samples had a lower density and expression than the ejaculate samples. Expression was not obtained, except for IZUMO1, from samples that underwent <i>in vitro</i> capacitation/acrosome reactions. Expression of IZUMO1 was seen as an increasing band formation from the equatorial region through the acrosome, after <i>in vitro</i> capacitation. However, after the acrosome reaction, the band formation was only on the equatorial region. Region-specific differences of proteins at the kDa level were obtained using Western blot analysis and possible isoforms specific to ram spermatozoa or proteins with similar epitopes were expressed. Considering the changes in surface proteins in frozen-thawed sperm, it is suggested that fertilin β and P34H can be used as fertility or freezability markers.</p>","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"470-482"},"PeriodicalIF":1.6,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38888040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vulnerabilities of Cancer Patients and Their Effects on Informed Consent for Biobanking.","authors":"Mason Kyle,&nbsp;Diana Cortez,&nbsp;Blaze Carbonell,&nbsp;Edgar Masmila,&nbsp;Alfredo Molinolo,&nbsp;Sharmeela Kaushal","doi":"10.1089/bio.2020.0156","DOIUrl":"https://doi.org/10.1089/bio.2020.0156","url":null,"abstract":"","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"543-548"},"PeriodicalIF":1.6,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39275214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Governance of Access in Biobanking: The Case of Telethon Network of Genetic Biobanks.","authors":"Sofia Iacomussi,&nbsp;Lorena Casareto,&nbsp;Manuela Locatelli,&nbsp;Chiuhui Mary Wang,&nbsp;Simona Borroni,&nbsp;Deborah Mascalzoni,&nbsp;Luca Sangiorgi","doi":"10.1089/bio.2021.0057","DOIUrl":"https://doi.org/10.1089/bio.2021.0057","url":null,"abstract":"<p><p>The discussion concerning the measure of the quality of a biobank should focus not only on the number of stored samples and their quality but also on the assessment of their access arrangements and governance. This article aims at contributing to the ongoing debate on samples and data access governance in biobanking by presenting the case of the Telethon Network of Genetic Biobanks (TNGB). We attempt to contribute to the need for clear and available access criteria and harmonization in access arrangements to maximize the influence of biobanks in the progress of biomedical research. We reviewed all the sample requests submitted to the TNGB from 2008 to 2020, focusing on those rejected by the Access Committee and the reasons behind the rejections. The analysis of the reasons behind the rejected requests allowed us to analyze how those relate to the issues of scientific misconduct, prioritization, and noncompliance with the biobank's mission. We discuss those issues in light of the actions and motivations used by TNGB in the access decision-making process. Based on this analysis, we suggest that a cross-implementation of a checklist for access assessment would improve the whole access process, ensuring a more transparent and smoother governance. Finally, we conclude that the TNGB's Charter and approach toward access governance could contribute as an important reference point to deal with the issues that have emerged in the international discussion on the topic.</p>","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"483-492"},"PeriodicalIF":1.6,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39949973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Efficient Culture Method of CD3-Positive T Cells from Human Cryopreserved Buffy Coat Specimens.","authors":"Mitsuhi Hirata,&nbsp;Michitaka Masuda,&nbsp;Michio Noguchi,&nbsp;Tsutomu Tomita,&nbsp;Hatsue Ishibashi-Ueda","doi":"10.1089/bio.2020.0031","DOIUrl":"https://doi.org/10.1089/bio.2020.0031","url":null,"abstract":"<p><p><b><i>Context:</i></b> In the National Cerebral and Cardiovascular Center (NCVC) Biobank, buffy coats have been collected from patients and stored with cryoprotective agents as a possible source for viable blood cells, using cost-efficient methods for storage. However, whether viable cells for <i>in vitro</i> studies can be recovered from these biospecimens has not been verified. <b><i>Objective:</i></b> To investigate whether T cells can be collected and expanded as viable cells from cryopreserved human buffy coats. <b><i>Design:</i></b> After thawing of cryopreserved buffy coat specimens, CD3-positive cells were isolated from the cell suspension using a leukocyte separation filter coated with an anti-CD3 antibody, and the filter-attached cells were cultured in T cell culture medium. To analyze the characteristics of these cultured cells, histocytological analyses of Giemsa staining, immunocytochemical (ICC) staining for CD3, and flow cytometry for CD3 in live cells were conducted. <b><i>Results:</i></b> A few days after starting cell culture, cell clusters were observed, and they gradually grew in size. Using Giemsa staining, the expanded cells were found to be ∼15 μm in diameter, having round nuclei, a high nucleus/cytoplasm ratio, and cytoplasm stained light blue, which is characteristic of lymphocytes. From ICC staining, these cells were CD3 positive, a pan-T cell marker among lymphocytes. Furthermore, CD3 immunoreactivity in live cells was detected in a flow cytometry assay, though that for CD19 was not detected, which is a marker of pan-B cells. <b><i>Conclusions:</i></b> These results suggest that T cells can be expanded from buffy coats cryopreserved at -180°C as an adequate method of NCVC Biobank, highlighting these biospecimens as a possible useful source for future <i>in vitro</i> studies.</p>","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"178-183"},"PeriodicalIF":1.6,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/bio.2020.0031","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38711326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Data Sharing in Biomedical Sciences: A Systematic Review of Incentives.","authors":"Thijs Devriendt,&nbsp;Mahsa Shabani,&nbsp;Pascal Borry","doi":"10.1089/bio.2020.0037","DOIUrl":"https://doi.org/10.1089/bio.2020.0037","url":null,"abstract":"<p><p><b><i>Background:</i></b> The lack of incentives has been described as the rate-limiting step for data sharing. Currently, the evaluation of scientific productivity by academic institutions and funders has been heavily reliant upon the number of publications and citations, raising questions about the adequacy of such mechanisms to reward data generation and sharing. This article provides a systematic review of the current and proposed incentive mechanisms for researchers in biomedical sciences and discusses their strengths and weaknesses. <b><i>Methods:</i></b> PubMed, Web of Science, and Google Scholar were queried for original research articles, editorials, and opinion articles on incentives for data sharing. Articles were included if they discussed incentive mechanisms for data sharing, were applicable to biomedical sciences, and were written in English. <b><i>Results:</i></b> Although coauthorship in return for the sharing of data is common, this might be incompatible with authorship guidelines and raise concerns over the ability of secondary analysts to contest the proposed research methods or conclusions that are drawn. Data publication, citation, and altmetrics have been proposed as alternative routes to credit data generators, which could address these disadvantages. Their primary downsides are that they are not well-established, it is difficult to acquire evidence to support their implementation, and that they could be gamed or give rise to novel forms of research misconduct. <b><i>Conclusions:</i></b> Alternative recognition mechanisms need to be more commonly used to generate evidence on their power to stimulate data sharing, and to assess where they fall short. There is ample discussion in policy documents on alternative crediting systems to work toward Open Science, which indicates that that there is an interest in working out more elaborate metascience programs.</p>","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"219-227"},"PeriodicalIF":1.6,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38864821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How Semantics Connotations May Influence Concerns About Donation of Biospecimens.","authors":"Stacey A Page,&nbsp;Beverly Anne Collisson,&nbsp;Jenny Godley,&nbsp;Danny Nguyen,&nbsp;Luanne Metz,&nbsp;Daniel Muruve","doi":"10.1089/bio.2020.0072","DOIUrl":"https://doi.org/10.1089/bio.2020.0072","url":null,"abstract":"<p><p><b><i>Introduction:</i></b> Human biological specimen (biospecimen) donation is routinely requested for clinical care and research purposes. Successfully engaging patients and research participants in biospecimen donation depends on what they understand these initiatives entail, including their perceptions of risk. Human biospecimens are stored in facilities routinely referenced as biobanks or biorepositories, both of which labels are known to embody a variety of connotations. The words chosen to describe biospecimen facilities may influence decisions about donation. <b><i>Objective:</i></b> To explore differences in likelihood of donation as a function of the words chosen to represent human biospecimen storage facilities and the commensurate concerns each label evokes. <b><i>Materials and Methods:</i></b> Two-group experimental design. Participants completed a survey about a fictitious undertaking requesting that they consider biospecimen donation. The term used to describe the facility housing the biospecimens differed; one half of the surveys referenced a biobank, and one half referenced a biorepository. <b><i>Results:</i></b> Two thousand five hundred ninety-six surveys were distributed; 586 completed surveys were received (response rate: 22.6%). Sixty-three percent of respondents, regardless of whether the label referenced a biobank or biorespository, reported being extremely likely to donate. There were no significant differences between the 2 groups on the 11 concerns sampled. Factor analyses revealed that concerns could be classified in two groups: use-related concerns and person-related concerns. The label biobank evoked significantly lower perception of importance of the person-related concerns sampled (e.g., personal or other benefit, discomfort or inconvenience). <b><i>Conclusions:</i></b> Our results suggest that researchers may consider using the word biobank to describe the facility housing the biospecimen, as this term appears less subject to concern biases. These outcomes confirm that misunderstandings or misattributions of words used to refer to biospecimen facilities could deter participation in clinical care or research. Participation may be enhanced through ensuring clear understanding of what biospecimen donation entails and by directly addressing common semantic misunderstandings and associations.</p>","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"156-162"},"PeriodicalIF":1.6,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/46/98/bio.2020.0072.PMC8217592.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38592303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Asphaltum Improves the Post-thaw Quality and Antioxidant Status of Nili Ravi Buffalo Bull Sperm.","authors":"Jaweria Sultan,&nbsp;Muhammad Amjad Awan,&nbsp;Bushra Allah Rakha,&nbsp;Syeda Anila Waqar,&nbsp;Muhammmad Sajjad Ansari,&nbsp;Sadia Naz,&nbsp;Sajid Iqbal,&nbsp;Shamim Akhter","doi":"10.1089/bio.2020.0033","DOIUrl":"https://doi.org/10.1089/bio.2020.0033","url":null,"abstract":"<p><p>Asphaltum, a mineral exudate from the mountains, is an ayurvedic medicine believed to be a panacea for male reproductive health issues. The objective of the study was to evaluate asphaltum in terms of phytochemical components, radical scavenging activity (RSA), <i>in vitro</i> dose tolerability, and cryosurvivability of buffalo sperm. Asphaltum was procured from an authentic source and confirmed for the presence of flavonoids, terpenoids, saponins, tannins, alkaloids, steroids, and glycosides. It showed good RSA as confirmed by the DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. <i>In vitro</i> dose tolerability of buffalo sperm (<i>n</i> = 3, replicate = 4, ejaculates = 24) for asphaltum was assessed at 0.75%, 1.5%, 2.25%, 3.0%, 3.75%, 4.5%, 5.25%, and 6.0% (w/v). Buffalo sperm showed good tolerance up to 3% of asphaltum in terms of sperm progressive motility and plasma membrane integrity. Buffalo semen (<i>n</i> = 3, replicates = 4, ejaculates = 24) was cryopreserved in extender supplemented with 0.0%, 0.75%, 1.5%, 2.25%, and 3.0% (w/v) asphaltum and sperm quality was assessed at post-dilution, post-cooling, and post-thaw. After dilution motility, viability and livability; post-cooling motility and plasma membrane integrity; and post-thaw motility, plasma membrane integrity, viability, livability, DNA integrity, sperm RSA, sperm total lipids, sperm mitochondrial activity, and total antioxidant activity of semen were improved by 3%. In conclusion, asphaltum supplementation in an extender at 3% improves the post-thaw quality and antioxidant activity of buffalo semen.</p>","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"194-203"},"PeriodicalIF":1.6,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38871583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryopreservation of Porcine Embryos: Recent Updates and Progress.","authors":"Du Xingzhu,&nbsp;Zhuan Qingrui,&nbsp;Cheng Keren,&nbsp;Luo Yuxi,&nbsp;Hou Yunpeng,&nbsp;Zhu Shien,&nbsp;Fu Xiangwei","doi":"10.1089/bio.2020.0074","DOIUrl":"https://doi.org/10.1089/bio.2020.0074","url":null,"abstract":"<p><p>Cryopreservation of embryos is important for long-distance embryo transfer and conservation of genetic resources. Porcine research is important for animal husbandry and biomedical research. However, porcine embryos are difficult to cryopreserve because of their high cytoplasmic lipid content and sensitivity to chilling stress. Vitrification is more efficient than slow freezing, and vitrification is mostly used in embryo cryopreservation. So far, the vitrification process of porcine embryos has been continuously improved, resulting in improved survival rates of warmed embryos and farrowing rates after the transplant procedure. It is worth noting that automatic vitrification has made great progress, which is expected to promote the standardization and application of vitrification. In this article, the vitrification process of porcine embryos at the blastula stage and early development stages is reviewed in detail. In addition, the efficiency of different vitrification systems was compared. In addition, we summarize technology that can improve the survival rate of cryopreserved porcine embryos, such as delipidation methods (including physical delipidation and chemical delipidation) and medium improvements (including chemically defined media and adding antioxidants). Meanwhile, gene expression changes during cryopreservation are also elaborated.</p>","PeriodicalId":49231,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"210-218"},"PeriodicalIF":1.6,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25400626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}