Cell Journal最新文献

{"title":"Identification and Functional Characterization of PI3K/Akt/mTOR Pathway-Related lncRNAs in Lung Adenocarcinoma: A Retrospective Study.","authors":"Jiaqi Zhong, Ying Kong, Ruming Li, Minghan Feng, Liming Li, Xiao Zhu, Lianzhou Chen","doi":"10.22074/cellj.2023.2007918.1378","DOIUrl":"10.22074/cellj.2023.2007918.1378","url":null,"abstract":"<p><strong>Objective: </strong>This paper aimed to investigate the PI3K/Akt/mTOR signal-pathway regulator factor-related lncRNA signatures (PAM-SRFLncSigs), associated with regulators of the indicated signaling pathway in patients with lung adenocarcinoma (LUAD) undergoing immunotherapy.</p><p><strong>Materials and methods: </strong>In this retrospective study, we employed univariate Cox, multivariate Cox, and least absolute shrinkage and selection operator (LASSO) regression analyses to identify prognostically relevant long non-coding RNAs (lncRNAs), construct prognostic models, and perform Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. Subsequently, immunoassay and chemotherapy drug screening were conducted. Finally, the prognostic model was validated using the Imvigor210 cohort, and tumor stem cells were analyzed.</p><p><strong>Results: </strong>We identified seven prognosis-related lncRNAs (<i>AC084757.3, AC010999.2, LINC02802, AC026979.2, AC024896.1, LINC00941</i> and <i>LINC01312</i>). We also developed prognostic models to predict survival in patients with LUAD. KEGG enrichment analysis confirmed association of LUAD with the PI3K/Akt/mTOR signaling pathway. In the analysis of immune function pathways, we discovered three good prognostic pathways (Cytolytic_activity, Inflammation-promoting, T_cell_co-inhibition) in LUAD. Additionally, we screened 73 oncology chemotherapy drugs using the \"pRRophetic\" algorithm.</p><p><strong>Conclusion: </strong>Identification of seven lncRNAs linked to regulators of the PI3K/Akt/mTOR signaling pathway provided valuable insights into predicting the prognosis of LUAD, understanding the immune microenvironment and optimizing immunotherapy strategies.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10864771/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139730816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Candidate Biomarkers for Targeting in Type 1 Diabetes; A Bioinformatic Analysis of Pancreatic Cell Surface Antigens.","authors":"Hamed Dabiri, Mahdi Habibi-Anbouhi, Vahab Ziaei, Zahra Moghadasi, Majid Sadeghizadeh, Ensiyeh Hajizadeh-Saffar","doi":"10.22074/cellj.2023.1996297.1262","DOIUrl":"10.22074/cellj.2023.1996297.1262","url":null,"abstract":"<p><strong>Objective: </strong>Type 1 diabetes (T1Ds) is an autoimmune disease in which the immune system invades and destroys insulin-producing cells. Nevertheless, at the time of diagnosis, about 30-40% of pancreatic beta cells are healthy and capable of producing insulin. Bi-specific antibodies, chimeric antigen receptor regulatory T cells (CAR-Treg cells), and labeled antibodies could be a new emerging option for the treatment or diagnosis of type I diabetic patients. The aim of the study is to choose appropriate cell surface antigens in the pancreas tissue for generating an antibody for type I diabetic patients.</p><p><strong>Materials and methods: </strong>In this bioinformatics study, we extracted pancreas-specific proteins from two large databases; the Human Protein Atlas (HPA) and Genotype-Tissue Expression (GTEx) Portal. Pancreatic-enriched genes were chosen and narrowed down by Protter software for the investigation of accessible extracellular domains. The immunohistochemistry (IHC) data of the protein atlas database were used to evaluate the protein expression of selected antigens. We explored the function of candidate antigens by using the GeneCards database to evaluate the potential dysfunction or activation/hyperactivation of antigens after antibody binding.</p><p><strong>Results: </strong>The results showed 429 genes are highly expressed in the pancreas tissue. Also, eighteen genes encoded plasma membrane proteins that have high expression in the microarray (GEO) dataset. Our results introduced four structural proteins, including NPHS1, KIRREL2, GP2, and CUZD1, among all seventeen candidate proteins.</p><p><strong>Conclusion: </strong>The presented antigens can potentially be used to produce specific pancreatic antibodies that guide CARTreg, bi-specific, or labeling molecules to the pancreas for treatment, detection, or other molecular targeted therapy scopes for type I diabetes.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10864774/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139730811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Mutational Hotspot in The <i>LAMP2</i> Gene: Unravelling Intrafamilial Phenotypic Variation and Global Distribution of The c.877C>T Variant: A Descriptive Study.","authors":"Saeideh Kavousi, Mohammad Dalili, Bahareh Rabbani, Mehrdad Behmanesh, Mehrdad Noruzinia, Nejat Mahdieh","doi":"10.22074/cellj.2023.2007469.1372","DOIUrl":"10.22074/cellj.2023.2007469.1372","url":null,"abstract":"<p><strong>Objective: </strong>Danon disease is defined by a clinical trio of cardiomyopathy, skeletal myopathy, and cognitive impairment. It results from the lysosomal-associated membrane protein-2 (<i>LAMP2</i>) gene variants. The aim of study is determination of genotype and phenotype of a newly diagnosed Iranian family with a unique phenotype due to a pathogenic variant of the <i>LAMP2</i> gene along with a phenotypic comparison of all reported patients.</p><p><strong>Materials and methods: </strong>In this descriptive study, we evaluated the demographic data, clinical features, management procedures, as well as genetic analysis of both patients in this newly diagnosed family. Whole genome sequencing (WGS) and in silico structural and functional predictions were applied. A comprehensive search of the c.877C>T variant in <i>LAMP2</i> was conducted using the PubMed, Google Scholar, VarSome, ClinVar, Human Gene Mutation Database (HGMD), and Franklin databases to identify any genotype-phenotype correlations.</p><p><strong>Results: </strong>Nine patients were carriers of the c.877C>T variant. All patients were male, and displayed variable degrees of left ventricular hypertrophy (LVH) that ranged from mild to severe. All patients exhibited typical cardiac conduction abnormalities consistent with Danon disease. Four underwent heart transplants and survived. Skeletal muscle involvement and cognitive impairment were observed in four patients each. The mean age of onset was 14 years. The proband in this study exhibited an earlier onset of cardiac symptoms.</p><p><strong>Conclusion: </strong>Genetic analysis is the preferred diagnosis approach for Danon disease and can assist families in managing affected patients, identify carriers, and assist with future family planning. This study highlights the intrafamilial phenotypic variability of Danon disease. It is possible that variants of this gene may be frequent in Iran.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10864773/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139730810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Implications and Prognostic Value of Leucine-Rich G Protein-Coupled Receptor 5 Expression as A Cancer Stem Cell Marker in Malignancies: A Systematic Review and Meta-Analysis.","authors":"Sepideh Ghobakhloo, Mehri Khoshhali, Nasimeh Vatandoost, Sima Jafarpour, Anoosha Niazmand, Reza Nedaeinia, Rasoul Salehi","doi":"10.22074/cellj.2023.2010157.1396","DOIUrl":"10.22074/cellj.2023.2010157.1396","url":null,"abstract":"<p><p>Leucine-rich G protein-coupled receptor 5 (<i>LGR5</i>) is a marker of cancer stem cells (CSCs) in various cancers. Based on different studies, conflicting reports exist on correlation between LGR5 expression and poor prognosis/ clinicopathological parameters in cancer patients. Therefore, our purpose in conducting this study was to investigate correlation between <i>LGR5</i> expression and outcomes of cancer patients under study through a systematic review and meta-analysis. Relevant articles were searched and collected using EMBASE, PubMed, Science Direct, and Scopus databases until December 21, 2022. This study was conducted to examine correlation between <i>LGR5</i> expression and different clinical outcomes, such as recurrence-free survival (RFS), disease-free survival (DFS), overall survival (OS), and clinicopathological characteristics of the included cancer patients. To achieve this, hazard ratios (HRs) with 95% confidence intervals (CIs) and odds ratios (ORs) with 95% CIs were used as statistical measures. A meta-analysis was conducted using STATA 12.0 software. Finally, 53 studies including 9523 patients met the inclusion criteria. Significantly, high-level expression of <i>LGR5</i> was related to poor prognosis in terms of OS, higher tumor stage, presence of distant metastasis, and presence of lymph node metastasis. It was discovered through subgroup analysis that several factors, including the study area, evaluation method, and type of cancer, can influence the correlation between <i>LGR5</i> expression and negative prognosis in cancer patients. According to the results of our study, <i>LGR5</i> overexpression was related to poor OS in cancer patients. In addition, clinicopathological data indicated an unfavorable prognosis in cancer patients with high <i>LGR5</i> expression. In conclusion, <i>LGR5</i> may serve as a potential prognostic marker for predicting survival in certain cancer types.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10864775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139730812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of Rosuvastatin-Incorporated Polycaprolactone -Gelatin Scaffold for Bone Repair: A Preliminary <i>In Vitro</i> Study.","authors":"Maliheh Gharibshahian, Morteza Alizadeh, Mohammad Kamalabadi Farahani, Majid Salehi","doi":"10.22074/cellj.2023.2009047.1391","DOIUrl":"10.22074/cellj.2023.2009047.1391","url":null,"abstract":"<p><strong>Objective: </strong>Rosuvastatin (RSV) is a hydrophilic, effective statin with a long half-life that stimulates bone regeneration. The present study aims to develop a new scaffold and controlled release system for RSV with favourable properties for bone tissue engineering (BTE).</p><p><strong>Materials and methods: </strong>In this experimental study, high porous polycaprolactone (PCL)-gelatin scaffolds that contained different concentrations of RSV (0 mg/10 ml, 0.1 mg/10 ml, 0.5 mg/10 ml, 2.5 mg/10 ml, 12.5 mg/10 ml, and 62.5 mg/10 ml) were fabricated by the thermally-induced phase separation (TIPS) method. Mechanical and biological properties of the scaffolds were evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), compressive strength, porosity, MTT, alkaline phosphatase (ALP) activity, water contact angle, degradation rate, pH alteration, blood clotting index (BCI), and hemocompatibility.</p><p><strong>Results: </strong>SEM analysis confirmed that the porous structure of the scaffolds contained interconnected pores. FTIR results showed that the RSV structure was maintained during the scaffold's fabrication. RSV (up to 62.5 mg/10 ml) increased compressive strength (16.342 ± 1.79 MPa), wettability (70.2), and degradation rate of the scaffolds. Scaffolds that contained 2.5 mg/10 ml RSV had the best effect on the human umbilical cord mesenchymal stem cell (HUC-MSCs) survival, hemocompatibility, and BCI. As a sustained release system, only 31.68 ± 0.1% of RSV was released from the PCL-Gelatin-2.5 mg/10 ml RSV scaffold over 30 days. In addition, the results of ALP activity showed that RSV increased the osteogenic differentiation potential of the scaffolds.</p><p><strong>Conclusion: </strong>PCL-Gelatin-2.5 mg/10 ml RSV scaffolds have favorable mechanical, physical, and osteogenic properties for bone tissue and provide a favorable release system for RSV. They can mentioned as a a promising strategy for bone regeneration that should be further assessed in animals and clinical studies.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10864776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139730815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between Genetic Polymorphism of The lncRNA <i>MIAT</i> rs1894720 with Ischemic Stroke Risk and lncRNA <i>MIAT</i> Expression Levels in The Blood after An Ischemic Stroke: A Case-Control Study.","authors":"Tahereh Asadabadi, Mohammad Javad Mokhtari, Mahnaz Bayat, Anahid Safari, Afshin Borhani-Haghighi","doi":"10.22074/cellj.2023.2003573.1315","DOIUrl":"10.22074/cellj.2023.2003573.1315","url":null,"abstract":"<p><strong>Objective: </strong>Genetic aspects can play an essential role in the occurrence and development of ischemic stroke (IS). Rs1894720 polymorphism is one of the eight single nucleotide polymorphisms (SNPs) in the long non-coding RNA (lncRNA) myocardial infarction-associated transcript (<i>MIAT</i>) locus. The aim of study is the lncRNA <i>MIAT</i> rs1894720 polymorphism decreases IS risk by reducing lncRNA <i>MIAT</i> expression.</p><p><strong>Materials and methods: </strong>In this case-control study, we studied 232 Iranian patients and 232 controls. The blood samples were collected from patients admitted at different times after stroke symptoms. We enrolled 80, 78, and 74 patients who arrived at the hospital between 0-24, 24-48, and 48-72 hours after the first appearance of symptoms, respectively. DNA genotyping was done by the tetra-primer ARMS-PCR method. Circulating MIAT levels were evaluated by real-time polymerase chain reaction (PCR).</p><p><strong>Results: </strong>The GT genotype of <i>MIAT</i> rs1894720 showed a significant association with the risk of IS (OR=3.53, 95% CI=2.13-5.84, P<0.001). <i>MIAT</i> expression was higher relative to the control within the first hours after IS. The <i>MIAT</i> levels in IS patients with rs1894720 (GT) were significantly lower relative to patients who had the GG and TT genotypes. Linear regression model indicated a significant correlation between <i>MIAT</i> expression with atherosclerotic risk factors and types of stroke in IS patients. Receiver operating characteristic (ROC) curve analysis showed that the level of lncRNA <i>MIAT</i> after IS could be diagnostic with an area under the curve (AUC) of 0.82. The sensitivity and specificity were 80.17 and 67.24%, respectively (P<0.001).</p><p><strong>Conclusion: </strong>Our study demonstrated that the <i>MIAT</i> rs1894720 polymorphism (GT) might increase the risk of IS in the Iranian population. <i>MIAT</i> expression was up-regulated in our IS patients. Hence, it could be a diagnostic biomarker for IS.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10777317/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139404896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Evaluation of Collagen-Induced Arthritis in Female Lewis Rats: A Comprehensive Analysis of Disease Progression and Severity.","authors":"Mahnaz Babaahmadi, Nima Makvand Gholipour, Behnoosh Tayebi, Jed Pheneger, Ensiyeh Hajizadeh-Saffar, Mohamadreza Baghaban Eslaminejad, Seyedeh-Nafiseh Hassani","doi":"10.22074/cellj.2023.2004504.1326","DOIUrl":"10.22074/cellj.2023.2004504.1326","url":null,"abstract":"<p><strong>Objective: </strong>The collagen-induced arthritis (CIA) model is the most commonly studied autoimmune model of rheumatoid arthritis (RA). In this study, we investigated the usefulness of collagen type II emulsified in Freund's incomplete adjuvant (CII/IFA) as a suitable method for establishing RA in Lewis rats. The aim of the present study was to present a straightforward and effective method for inducing CIA in rats.</p><p><strong>Materials and methods: </strong>In this experimental study, animals were divided into two equal groups (n=5); control and CIA. Five rats were injected intradermally at the base of the tail with a 0.2 ml CII/IFA emulsion. On the seventh day, a 0.1 ml CII/IFA emulsion booster was injected. Arthritis symptoms that arose were evaluated at clinical, histological, radiological, and at protein expression levels to find out if the disease had been induced successfully.</p><p><strong>Results: </strong>Our finding showed a decreasing trend in the body weight during the RA induction period, while the arthritis score and paw thickness were increased during this period. The results of the enzyme-linked immunosorbent assay (ELISA) for serum samples revealed that the levels of proinflammatory cytokines, interleukin (IL)-1β, IL-6, IL-17, and tumor necrosis factor (TNF)-α and anti-CII IgG were significantly increased in CIA rats compared to the control group. After CIA induction, the level of anti-inflammatory protein IL-10 was decreased significantly. Radiographic examination of the hind paws showed soft tissue swelling, bone erosion, and osteophyte formation in CIA rats. Additionally, based on histological evaluations, the hind paws of the CIA group showed pannus formation, synovial hyperplasia, and bone and cartilage destruction.</p><p><strong>Conclusion: </strong>It seems that CII/IFA treatment can be an appropriate and effective method to induce RA disease in Lewis rats. This well-established and well-characterized CIA model in female Lewis rats could be considered to study aspects of RA and develop novel anti-arthritic agents.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10777318/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139404897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated Bioinformatic Analysis of Differentially Expressed Genes Associated with Wound Healing.","authors":"Mansoureh Farhangniya, Farzaneh Mohamadi Farsani, Najmeh Salehi, Ali Samadikuchaksaraei","doi":"10.22074/cellj.2023.2007217.1368","DOIUrl":"10.22074/cellj.2023.2007217.1368","url":null,"abstract":"<p><strong>Objective: </strong>Wound healing is a complex process involving the coordinated interaction of various genes and molecular<br />pathways. The study aimed to uncover novel therapeutic targets, biomarkers and candidate genes for drug development<br />to improve successful wound repair interventions.<br />Materials and Methods: This study is a network-meta analysis study. Nine wound healing microarray datasets obtained<br />from the Gene Expression Omnibus (GEO) database were used for this study. Differentially expressed genes (DEGs)<br />were described using the Limma package and shared genes were used as input for weighted gene co-expression<br />network analysis. The Gene Ontology analysis was performed using the EnrichR web server, and construction of a<br />protein-protein interaction (PPI) network was achieved by the STRING and Cytoscape.<br />Results: A total of 424 DEGs were determined. A co-expression network was constructed using 7692 shared genes<br />between nine data sets, resulting in the identification of seven modules. Among these modules, those with the top 20<br />genes of up and down-regulation were selected. The top down-regulated genes, including TJP1, SEC61A1, PLEK,<br />ATP5B, PDIA6, PIK3R1, SRGN, SDC2, and RBBP7, and the top up-regulated genes including RPS27A, EEF1A1,<br />HNRNPA1, CTNNB1, POLR2A, CFL1, CSNk1E, HSPD1, FN1, and AURKB, which can potentially serve as therapeutic<br />targets were identified. The KEGG pathway analysis found that the majority of the genes are enriched in the \"Wnt<br />signaling pathway\".<br />Conclusion: In our study of nine wound healing microarray datasets, we identified DEGs and co-expressed modules<br />using WGCNA. These genes are involved in important cellular processes such as transcription, translation, and posttranslational<br />modifications. We found nine down-regulated genes and ten up-regulated genes, which could serve as<br />potential therapeutic targets for further experimental validation. Targeting pathways related to protein synthesis and cell<br />adhesion and migration may enhance wound healing, but additional experimental validation is needed to confirm the<br />effectiveness and safety of targeted interventions.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10777322/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139404899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of Lycopene on Modulating Oxidative Stress and Liver Enzymes Levels in Metabolic Syndrome Patients: A Randomised Clinical Trial.","authors":"Mahdi Mirahmadi, Malihe Aghasizadeh, Fatemeh Nazifkar, Mahla Ghafarian Choubdari, Reza Assaran-Darban, Shima Tavallaie, Hossein Hatamzadeh, Gordon Ferns, Mohammad Reza Mirinezhad, Hamed Baharara, Farzin Hadizadeh, Majid Ghayour-Mobarhan","doi":"10.22074/cellj.2023.2006158.1353","DOIUrl":"10.22074/cellj.2023.2006158.1353","url":null,"abstract":"<p><strong>Objective: </strong>The pathogenesis of metabolic syndrome (MetS) complications involves the excessive production of<br />reactive oxygen species, inflammation, and endothelial dysfunction. Due to Lycopene, a highly unstable structure and<br />its significant effects on modulating the metabolic system, there is a strong need for a formula that can increase its<br />stability. The aim of this study was to develop an approach for encapsulating Lycopene and investigate its effects on<br />inflammatory markers, oxidative stress, and liver enzymes in patients with MetS.<br />Materials and Methods: This study is a simple randomized, double-blind, objective-based clinical trial that involved<br />eighty subjects with MetS, who were equally and randomly assigned to two groups: one group received 20 mg of<br />Lycopene per day for 8 weeks, and the Placebo group followed the same protocol as the Lycopene group but received<br />a placebo instead of Lycopene. They were called Lycopene and placebo, respectively. During follow-up visits after 4<br />and 8 weeks, 20 ml of blood was collected for evaluation of liver enzymes and some inflammatory related markers.<br />Results: Prior to the assignment of volunteers to their respective groups, there were no notable differences in C-reactive<br />protein (CRP), serum liver enzymes, systolic and diastolic blood pressure, or pro-oxidant-antioxidant balance (PAB)<br />between the Lycopene and placebo groups. However, our subsequent analysis revealed a significant reduction in the<br />serum levels of CRP (P=0.001) and PAB (P=0.004) in the group that received Lycopene. Our encapsulated Lycopene<br />treatment was not associated with a significant difference in serum levels of alanine aminotransferase (ALT), aspartate<br />transferase (AST), or alkaline phosphatase (ALP) between our two groups.<br />Conclusion: This study investigated the impact of Lycopene on individuals with MetS, revealing a noteworthy<br />modulation effect on PAB and inflammation linked to MetS. However, no significant differences was demonstrated in<br />serum levels of ALT, AST and ALP between the studied group (registration number: IRCT20130507013263N3).</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10777315/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139404925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Left Barrel Cortical Neurons Activity following Transplantation of Stem Cells into Right Lesioned-Barrel Cortex in Rats.","authors":"Mansoureh Sabzalizadeh, Mohammad Reza Afarinesh, Ali Derakhshani, Vahid Sheibani","doi":"10.22074/cellj.2023.2007586.1373","DOIUrl":"10.22074/cellj.2023.2007586.1373","url":null,"abstract":"<p><strong>Objective: </strong>Stem cells (SCs) can improve the functional defects of brain injury. Rodents use their whiskers to get tactile information from their surroundings. The aim of this study was to investigate whether the transplantation of SCs into the lesioned barrel cortex can help neuronal function in the contralateral cortex.</p><p><strong>Materials and methods: </strong>Sixteen male Wistar rats (200-230 g) were used in this experimental study. We induced a mechanical lesion in the right barrel cortex area of rats by removing this area by a 3 mm skin punch. Four groups containing one intact group of rats: group 1: control, and three lesion groups, group 2: lesion+un-differentiated dental pulp SCs (U-DPSCs), group 3: lesion+differentiated dental pulp SCs (D-DPSCs), and group 4: cell medium (vehicle) that were injected in the lesion area. Three weeks after transplantation of SCs or cell medium, the rats' responses of left barrel cortical neurons to controlled deflections of right whiskers were recorded by using the extracellular single-unit recordings technique.</p><p><strong>Results: </strong>The results showed that the neural spontaneous activity and response magnitude of intact barrel cortex neurons in the lesion group decreased significantly (P<0.05) compared to the control group while ON and OFF responses were improved in the D-DPSCs (P<0.001) group compared to the vehicle group three weeks after transplantation.</p><p><strong>Conclusion: </strong>Transplantation of dental pulp mesenchymal SCs significantly improved the neural responses of the left barrel cortex that was depressed in the vehicle group.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10777320/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139404900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}