白藜芦醇纳米制剂通过自噬诱导抑制侵袭性乳腺癌细胞生长：一项体外研究。

IF 1.7 4区生物学 Q4 CELL BIOLOGY

Cell Journal Pub Date : 2024-02-01 DOI:10.22074/cellj.2024.2016930.1458

Mohammad Rasool Khazaei, Maryam Bozorgi, Mozafar Khazaei, Maryam Aftabi, Azam Bozorgi

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引用次数: 0

摘要

研究目的本研究旨在合成壳聚糖纳米颗粒（Cs NPs），用于白藜芦醇（RSV）的递送，并评估其诱导MDA-MB 231细胞自噬的有效性：在本实验研究中，通过离子凝胶法制备了纯的和负载 RSV 的 Cs NPs（RSV.Cs NPs），使用标准技术表征了它们的理化性质，并在体外测量了 RSV 的释放。将 MDA-MB 231 细胞与 RSV、Cs NPs 和 RSV.根据 MTT 试验计算 Cs NPs 和半数最大抑制浓度（IC50）值。细胞活力通过乳酸脱氢酶（LDH）检测法进行评估，自噬通过实时聚合酶链反应（PCR）进行评估：傅立叶变换红外光谱分析证实了 NP 的形成。纯的和 RSV.Cs NPs 尺寸分别为 36.7 和 94.07 nm，zeta 电位分别为 18.3 和 27 mV。在 NPs 中夹带的 RSV 有 60% 以上在最初以猝灭的方式释放，随后在 72 小时内逐渐释放。铯和 RSV。Cs NPs 在较低浓度下抑制细胞增殖。RSV.Cs NPs表现出最高的抗癌效果，并刺激自噬，表现为Beclin-1 ATG5、ATG7、LC3A和P62的表达增加：结论：RSV.结论：RSV.Cs NPs 在体外通过诱导自噬抑制浸润性乳腺癌（BC）细胞方面表现出良好的效果。

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Resveratrol Nanoformulation Inhibits Invasive Breast Cancer Cell Growth through Autophagy Induction: An In Vitro Study.

Objective: The aim of this study was to synthesize chitosan nanoparticles (Cs NPs) for resveratrol (RSV) delivery and assess their effectiveness in inducing autophagy in MDA-MB 231 cells.

Materials and methods: In this experimental study, Pure and RSV-loaded Cs NPs (RSV. Cs NPs) were prepared via the ionic gelation method, and their physicochemical properties were characterized using standard techniques, and RSV release was measured in vitro. MDA-MB 231 cells were incubated with RSV, Cs NPs, and RSV. Cs NPs and Half-maximal inhibitory concentration (IC50) values were calculated following the MTT test. Cell viability was assessed by lactate dehydrogenase (LDH) assay, and autophagy was evaluated using the real-time polymerase chain reaction (PCR).

Results: NP formation was confirmed with the analysis of FTIR spectra. Pure and RSV. Cs NPs had 36.7 and 94.07 nm sizes with 18.3 and 27 mV zeta potentials, respectively. Above 60% of RSV entrapped within NPs was released in an initial burst manner followed by a gradual release till 72 hours. Cs and RSV. Cs NPs restrained cell proliferation at lower concentrations. RSV. Cs NPs showed the highest anticancer effect and stimulated autophagy, indicated by increased Beclin-1 ATG5, ATG7, LC3A, and P62 expression.

Conclusion: RSV. Cs NPs show promising effects in inhibiting invasive breast cancer (BC) cells in vitro by inducing autophagy.

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来源期刊

Cell Journal CELL BIOLOGY-

CiteScore

3.40

自引率

5.00%

发文量

审稿时长

12 months

期刊介绍： The “Cell Journal (Yakhteh)“, formerly published as “Yakhteh Medical Journal”, is a quarterly English publication of Royan Institute. This journal focuses on topics relevant to cellular and molecular scientific areas, besides other related fields. The Cell J has been certified by Ministry of Culture and Islamic Guidance in 1999 and was accredited as a scientific and research journal by HBI (Health and Biomedical Information) Journal Accreditation Commission in 2000 which is an open access journal.