Cell Journal最新文献

{"title":"Can Anticancer Drugs Be A Promising Candidate for The Treatment of Endometriosis?","authors":"Sedigheh Kamrani, Soodeh Mahdian, Maryam Shahhoseini","doi":"10.22074/cellj.2025.2037024.1635","DOIUrl":"https://doi.org/10.22074/cellj.2025.2037024.1635","url":null,"abstract":"<p><p>Endometriosis, a benign gynecological disorder affecting 10-15% of women during their reproductive years, is characterized by the growth of endometrial tissue outside the uterus. Despite its prevalence, the exact pathophysiology of this disease remains poorly understood. Current treatments, including surgery and hormonal therapies, often have limited efficacy and may be associated with significant side effects. In recent years, drug repurposing has emerged as a promising strategy for managing endometriosis. This approach capitalizes on the molecular similarities between endometriosis and certain cancers, particularly the role of proteins such as fibronectin. By targeting these shared molecular pathways, researchers are exploring the potential of repurposing existing drugs, especially anticancer agents, to treat endometriosis. This strategy promises to provide more effective and less invasive treatment options for patients with endometriosis. Preliminary studies have shown the potential of anticancer drugs in inhibiting disease progression and alleviating symptoms. However, further clinical trials are necessary to confirm these findings and establish the precise role of anticancer drugs in the management of endometriosis.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"26 10","pages":"619-621"},"PeriodicalIF":1.7,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143505496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stem Cell Perspective for Regenerative Wound Healing: from Biology toward Future Clinical Directions: A Review.","authors":"Nahid Nasiri, Fatemeh Haghdoost, Masoud Habibi, Mohsen Fateh, Shaghayegh Gholami, Seyed Mehdi Tabaie","doi":"10.22074/cellj.2024.2034492.1613","DOIUrl":"https://doi.org/10.22074/cellj.2024.2034492.1613","url":null,"abstract":"<p><p>Chronic wounds, a major clinical challenge, still need to develop new methods based on efficient technologies to improve treatment results. Stem cells, particularly mesenchymal stem cells (MSC), as an advanced approach in skin regenerative medicine, brought new hopes. The multifaceted effects of MSCs, including paracrine signaling, trophic factor secretion, and modulation of the wound microenvironment, orchestrate a cascade of regenerative, plays a critical role in tissue repair. Preclinical investigations have revealed the regenerative capacity of MSCs in accelerating wound closure, promoting angiogenesis, and fostering a pro-healing environment in chronic wound models. Clinical trials have also confirmed these findings and show the efficacy of MSC treatment in accelerating wound healing and improving the quality of healed tissue in patients with chronic wounds. Despite the therapeutic progress, key issues, such as optimal cell sourcing, cell dosage, delivery modalities, and long-term safety profiles, there are a number of unresolved issues which need to be dealt with. This review aims to provide a comprehensive overview of current state of stem cell research in wound healing, and offers a new new hope for effective and innovative treatments in regenerative medicine.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"26 10","pages":"575-589"},"PeriodicalIF":1.7,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143505505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stem Cell Isolation from Human Wharton's Jelly: A Study of Their Differentiation Ability into Lens Fiber Cells.","authors":"Seyedeh Mahsa Khatami, Saber Zahri, Masoud Malek, Kamaloddin Hamidi","doi":"10.22074/cellj.2025.721280","DOIUrl":"https://doi.org/10.22074/cellj.2025.721280","url":null,"abstract":"<p><p>In the article published in Cell J, Vol 15, No 4, 2015, pages 364-371, the reference for Table 1 and Figures 1B-D, 2, and 5A, B was inadvertently omitted. The missing reference (24), originally written in Persian by the authors, has now been added to the relevant Table and Figures' legends with the permission of the original publisher, Journal of Ardabil University of Medical Sciences. The authors would like to apologies for any inconvenience caused.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"26 10","pages":"622-624"},"PeriodicalIF":1.7,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143505503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Geographical Distribution of Global Biobanks.","authors":"Farzad Parvizpour, Sarvenaz Salahi, Mohammad Amin Mahmanzar, Karim Rahimian, Neda Esfandiari, Zahra Fatahi, Niousha Khoshdast, Reyhaneh Yarmohammadi, Mohamad Amir Amirkhani, Arefeh Jafarian","doi":"10.22074/cellj.2024.2024380.1525","DOIUrl":"https://doi.org/10.22074/cellj.2024.2024380.1525","url":null,"abstract":"<p><p>This study aimed to comprehensively review the global biobanks to visualize their geographical distribution. The protocol for this review consisted of the following steps: i. Developing a search strategy to identify biobanks from each continent, ii. Defining variables (such as tissue-based, cell-based, and gene-based biobanks) and organizing them in Excel sheets for data collection, iii. Collecting data, iv. Removing duplicate and invalid entries, v. Structuring the database, and vi. Analyzing the data. MATLAB software was utilized for data analysis and chart plotting. Data on global biobanks aimed to collected through targeted searches of databases, publications, and registries using predefined variables such as biobank type, location, and accessibility. The data were organized, cleaned to remove duplicates, and analyzed using MATLAB to visualize geographical distribution and prevalence patterns. Tissue and cell-based, tissue-based, and cellbased biobanks were the most common type of global biobanks with a prevalence of 30.4, 27.93, and 25.15%. United Kingdom (n=78, P=43.09%), Canada (n=43, P=23.75%), and the United States (n=33, P=18.23%) were the countries with a higher frequency of tissue-based biobanks (domain frequency: 1-78; 0.55-43.09%). However, tissue and genebased biobanks had the most minor frequency and were only in two countries of Spain (n=1, P=25%) and the United Kingdom (n=3, P=75%). The results of this study indicate that the feasibility of designing and conducting biobanks varies by type. Tissue and cell-based biobanks were found to be more prevalent, followed by tissue-based, cell-based, cell and gene-based, tissue, cell, and gene-based, gene-based, and finally, tissue and gene-based biobanks. This study represents the initial step in creating a global database by identifying all types of biobanks worldwide.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"26 9","pages":"523-529"},"PeriodicalIF":1.7,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143014784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interleukin-12 Inhibits Tumor Growth and Metastasis Promoted by Tumor-Associated Mesenchymal Stem Cells in Triple-Negative Breast Cancer.","authors":"Babak Jahangiri, Zahra-Soheila Soheili, Elahe Asadollahi, Mehdi Shamsara, Vahid Shariati, Alireza Zomorodipour","doi":"10.22074/cellj.2024.2036513.1634","DOIUrl":"https://doi.org/10.22074/cellj.2024.2036513.1634","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study was to understand the interactions between tumor-associated mesenchymal stem cells (TA-MSCs) and triple-negative breast cancer (TNBC) cells, which appear to be necessary for developing effective therapies.</p><p><strong>Materials and methods: </strong>In this experimental study, MDA-MB-231 and 4T1 TNBC cells were co-cultured with bone marrow-derived MSCs, and TA-MSCs conditioned media (CM) were collected. TA-MSC CM-treated TNBC cells were subjected to migration and invasion assays. Epithelial-mesenchymal transition (EMT) marker expression was quantified by real-time polymerase chain reaction (RT-PCR). Cell proliferation was measured using trypan blue exclusion technique, while cell cycle distribution and apoptosis were assessed by flow cytometry. The effects of TA-MSCs on tumor volume, survival rate, and lung metastasis were evaluated by subcutaneous co-injection of MSCs with 4T1 cells in the right flanks of BALB/c mice (n=5 per group). Intratumoral interleukin-12 (IL-12) immunotherapy was performed using lentiviral particles as a rescue experiment. The TA-MSCs RNA-seq dataset (PRJEB27694) was analyzed to detect elevated metastasis-associated oncogenes, downloaded from the European Nucleotide Archive database. For validation of the RNA-seq data analysis, the expression levels of candidate oncogenes were evaluated in TA-MSCs, TNBC cells, and tumor tissue using RT-PCR.</p><p><strong>Results: </strong>TA-MSCs enhanced migration, invasion, and EMT of TNBC cells in vitro without affecting cell proliferation or apoptosis. <i>In vivo</i>, TA-MSCs increased tumor growth and lung metastasis, while decreasing survival rates. IL-12 therapy elevated serum IL-12 and interferon-gamma (IFN-γ) expression, suppressed tumor volume and lung metastasis, and improved overall survival in the TA-MSC group. RNA-seq data analysis identified upregulated oncogenes in TA-MSCs, among which <i>MMP3, CXCL2, CXCL5</i>, and <i>ICAM1</i> were selected as the most relevant to metastasis. These genes showed increased expression in TA-MSCs, TNBC cells, and tumor tissues.</p><p><strong>Conclusion: </strong>The findings of the present study revealed a complex interplay between TA-MSCs and TNBC cells that affects tumor growth and metastasis. Preclinical results indicate that intratumoral IL-12 immunotherapy shows promise in overcoming TA-MSC-promoted tumor growth and metastasis.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"26 9","pages":"543-558"},"PeriodicalIF":1.7,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143014829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Quercetin and Exercise in Modulating Apoptosis and Cardiomyopathy via PI3K/AKT/FOXO3 Pathways in Diabetic Obese Rats.","authors":"Mojdeh Khajehlandi, Lotfali Bolboli","doi":"10.22074/cellj.2024.2042044.1682","DOIUrl":"https://doi.org/10.22074/cellj.2024.2042044.1682","url":null,"abstract":"<p><strong>Objective: </strong>Quercetin and exercise both have antidiabetic effects through decreasing blood glucose while increasing insulin sensitivity. Therefore, the present study aimed to investigate the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) exercises along with quercetin administration on apoptosis and cardiomyopathy in diabetic obese rats.</p><p><strong>Materials and methods: </strong>In this experimental study, 35 male Wistar rats [diabetic rats for experimental groups and normal rats for healthy control (HC)] were divided into seven groups (for each group n=5): HC, diabetic control (DC), diabetic quercetin control (DQC), diabetic HIIT (DHT), diabetic MICT (DMT), DHT with quercetin (DQHT) and DMT with quercetin (DQMT). The rats were fed a high-fat diet (HFD) for 8 weeks and a low dose of streptozotocin (STZ) was administered to create a model of type 2 diabetes mellitus (T2DM). Eight weeks of HIIT and MICT with or without quercetin treatment were performed. Quercetin was used at 15 mg/kg, as a suspension in carboxymethyl cellulose (CMC) at a concentration of 0.5%. One-way analysis of variance with LSD's post-hoc test with a significant level of P≤0.05 was used to analyze data.</p><p><strong>Results: </strong>Just 8 weeks of HIIT and MICT protected the protein content of PI3K, AKT, and FOXO3 and caspase-8 (<i>Casp- 8</i>) gene expression in heart tissues (P<0.05). Quercetin and both training protocols decreased blood glucose, while improving inflammatory markers and the lipid profile (P<0.05).</p><p><strong>Conclusion: </strong>Reduction in blood glucose along with improvements in the inflammatory markers and the lipid profile by quercetin injection may be a promising approach for the development of new antidiabetic medications. In addition, both training protocols showed potentially successful diabetic cardiomyopathy treatments through modulating the FOXO3 and PI3K/AKT pathways.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"26 9","pages":"559-568"},"PeriodicalIF":1.7,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143014786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxaliplatin-Loaded Chitosan Nanoparticles Decorated with Cetuximab Single-Chain Variable Fragment for Human Colorectal Cancer Treatment.","authors":"Khadijeh Falahzadeh, Fariba Esmaeili, Leila Nematollahi, Elham Bayat, Mehdi Khoobi, Mohammadali Mazloomi, Masumeh Jalalvand, Reza Faridi Majidi, Babak Negahdari","doi":"10.22074/cellj.2024.2033893.1607","DOIUrl":"https://doi.org/10.22074/cellj.2024.2033893.1607","url":null,"abstract":"<p><strong>Objective: </strong>Colorectal cancer (CRC) is the second leading cause of cancer-related deaths worldwide. Engineered biomolecules can be used as a targeted tool to deliver drugs directly to tumors that reduce the adverse effects of conventional treatments. We aimed to prepare non-targeted oxaliplatin-loaded chitosan nanoparticles (OXPT-CS NPs) and targeted OXPT-CS NPs decorated with cetuximab single-chain variable fragment (scFv) to send both NPs to epidermal growth factor receptor (EGFR) overexpressing HCT 116 cells, a human colorectal carcinoma cell line, for comparing their cytotoxicity.</p><p><strong>Materials and methods: </strong>In this experimental study, OXPT-CS NPs were synthesized using a fluid system. Encapsulation efficiency percentage (EE%) and oxaliplatin release rate were evaluated. Western blot and cell-based ELISA confirmed scFv production and its binding ability to EGFR, respectively. The Fourier transform infrared spectroscopy (FTIR) determined the conjugation of scFv to OXPT-CS NPs. The NPs were characterized, and their toxicity against the HCT 116 cells was evaluated using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) and flow cytometry assays.</p><p><strong>Results: </strong>The EE% of OXPT-CS NPs was 93%, and the average diameters were 75.85 ± 8.81 nm and 92.48 ± 9.51 before and after scFv conjugation, respectively. The scFv was purified via affinity chromatography. The western blot method and cell-based ELISA revealed successful purification of scFv and its attachment to EGFR on HCT 116 cells. The FTIR analysis determined the interactions between the scFv and OXPT-CS NPs. According to MTT and flow cytometry results, the targeted delivery system significantly reduced HCT 116 cancer cell viability and increased apoptosis induction up to 99.8%.</p><p><strong>Conclusion: </strong>The scFv-OXPT-CS NPs demonstrated an increased cytotoxic function due to the presence of scFv in its formulation. This delivery system offers a promising method for delivering chemotherapy drugs to cancer cells. More research is needed on the best strategies for improving treatment efficacy by targeting cancer cells.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"26 9","pages":"530-542"},"PeriodicalIF":1.7,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143014782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell-Based Therapy for Cerebral Palsy: A Puzzle in Progress.","authors":"Masoumeh Nouri, Morteza Zarrabi, Safdar Masoumi, Elaheh Khodadoust, Anahita Majmae, Man Amanat, Mahmoud Reza Ashrafi, Massoud Vosough","doi":"10.22074/cellj.2024.2032098.1600","DOIUrl":"https://doi.org/10.22074/cellj.2024.2032098.1600","url":null,"abstract":"<p><p>Cell-based therapy has shown promising outcomes in the treatment of cerebral palsy (CP). However, there is no consensus on a standard therapeutic protocol regarding the source of cells, optimal cell dose, timing and frequency of cell injections, route of administration, or the use of combination therapy. This lack of consensus necessitates a comprehensive investigation to clarify these crucial yet undefined factors in cell-based therapy for CP patients. In this commentary, we discuss and compare the trends in Gross Motor Function Measure-66 following intrathecal injection of umbilical cord blood mononuclear cells (UCB-MNCs) and umbilical cord tissue mesenchymal stromal cells (UCTMSCs) in children with CP. Our study revealed that MNC injections led to earlier improvements in gross motor function, whereas MSC applications resulted in more sustainable changes. These findings provide key insights into the efficacy of different cell types, which will be beneficial for future studies and for refining cell-based therapy protocols for CP treatment.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"26 9","pages":"569-574"},"PeriodicalIF":1.7,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143014827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dysregulated Long Non-Coding RNAs in Colorectal Cancer: Identification and Validation Using RNA-seq and Real-Time Reverse Transcription Polymerase Chain Reaction.","authors":"Reyhaneh Dehghanzad, Roghayeh Rahbar Parvaneh, Mohammad Keramatipour, Sepideh Kadkhoda, Mohsen Aghajanpour, Reza Taslimi, Leyla Sahebi, Abbas Shakoori Farahani","doi":"10.22074/cellj.2025.2042114.1684","DOIUrl":"https://doi.org/10.22074/cellj.2025.2042114.1684","url":null,"abstract":"<p><strong>Objective: </strong>Colorectal cancer (CRC) is the third most common cancer and the second leading cause of cancerrelated deaths worldwide, posing a significant public health challenge. Recent advances in molecular research and technologies have highlighted the potential of long non-coding RNAs (lncRNAs) as key players in CRC development and progression. High-throughput technologies, such as RNA sequencing (RNA-seq), enable comprehensive analysis of cancer omics data, providing valuable insights into CRC biology. This study aimed to identify and validate lncRNAs with potential roles in CRC pathogenesis.</p><p><strong>Materials and methods: </strong>In this experimental study, we conducted RNA-seq analysis using data from The Cancer Genome Atlas (TCGA) to identify differentially expressed lncRNAs between 481 CRC and 41 healthy control samples. Using the Boruta feature selection algorithm, we identified statistically significant lncRNAs. Based on a comprehensive literature review, four lncRNAs (<i>LINC01730, LINC02487, LINC01836,</i> and <i>LINC01594</i>) were selected for experimental validation. Real-time reverse transcription polymerase chain reaction (RT-PCR) was performed on 10 primary CRC and 9 normal colorectal tissue samples to quantify the expression of these lncRNAs. Additionally, bioinformatic analyses were conducted to explore molecular pathways associated with these lncRNAs in CRC.</p><p><strong>Results: </strong>RNA-seq analysis identified 388 differentially expressed lncRNAs in CRC tissues compared to healthy controls. Among these, four lncRNAs (<i>LINC01730, LINC02487, LINC01836,</i> and <i>LINC01594</i>) were validated through real-time RT-PCR, confirming their significant upregulation in CRC tissues. Bioinformatic analysis revealed their potential involvement in molecular pathways critical to tumor growth and metastasis.</p><p><strong>Conclusion: </strong>This study identified and validated four significantly upregulated lncRNAs (<i>LINC01730, LINC02487, LINC01836</i>, and <i>LINC01594</i>) in CRC tissues, providing evidence of their potential roles in CRC pathogenesis. These lncRNAs could serve as promising candidates for future biomarker development and therapeutic research in colorectal cancer.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"26 12","pages":"700-710"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144545787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Effects of <i>Althaea officinalis L.</i> and Metformin on Estradiol-Induced Polycystic Ovary Syndrome in Rats: Insights into The PI3K/AKT Pathway, Inflammation, and Oxidative Stress.","authors":"Jiefan Gao, Yue Huang, Yangyang Song, Xiao Huang, Dan Zhang, Zhimin Hou","doi":"10.22074/cellj.2024.2026329.1550","DOIUrl":"https://doi.org/10.22074/cellj.2024.2026329.1550","url":null,"abstract":"<p><strong>Objective: </strong>Polycystic ovary syndrome (PCOS) is one of the most important causes of infertility, irregular menstrual cycles, and anovulation in women. The current study aimed to investigate the therapeutic effects of <i>Althaea officinalis L.</i> (A. officinale) extract on PCOS in rats.</p><p><strong>Materials and methods: </strong>In this experimental study, 70 rats in 7 groups (n=10/group) were studied for three weeks as follows; healthy control (HC), patient (PCOS), metformin (PCOS+MET), <i>A. officinale</i> treatment (PCOS+250 and 500 mg/kg <i>A. officinale</i>) and synergistic (PCOS+MET+250 and 500 mg/kg <i>A. officinale</i>) groups. Luteinizing hormone (LH), folliclestimulating hormone (FSH), progesterone (P) and testosterone (T) levels as well as inflammatory cytokines were measured. Total antioxidant capacity and lipid peroxidation levels were analyzed in ovarian tissue. The expression of <i>GLUT-4, AKT, PI3K, PTEN genes</i> and <i>Ki-67</i> was assessed by real-time polymerase chain reaction (PCR) and immunohistochemistry, respectively.</p><p><strong>Results: </strong><i>A. officinale</i> alone and especially in combination with MET moderated inflammatory and antioxidant parameters compared to the PCOS and MET groups. <i>A. officinale</i> in synergistic groups increased the apoptosis of granulosa cells by activating the PI3K/AKT pathway, resulting in a rise in the number of Ki-67 positive cells (P<0.05). Furthermore, following <i>A. officinale</i> treatment the LH/FSH rate decreased and FSH and P increased (P<0.05). Also, A. officinale extract could effectively normalize estrus cycle duration close to the normal group.</p><p><strong>Conclusion: </strong>The extract of <i>A. officinale</i>, in combination with metformin, can enhance the hypothalamic-pituitaryovary (HPO) axis with synergistic anti-inflammatory and antioxidant effects. Additionally, the extract showed apoptotic effect on cystic granulosa cells.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"26 8","pages":"473-486"},"PeriodicalIF":1.7,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142394654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}