Cell Journal最新文献

{"title":"Fabrication of Rosuvastatin-Incorporated Polycaprolactone -Gelatin Scaffold for Bone Repair: A Preliminary <i>In Vitro</i> Study.","authors":"Maliheh Gharibshahian, Morteza Alizadeh, Mohammad Kamalabadi Farahani, Majid Salehi","doi":"10.22074/cellj.2023.2009047.1391","DOIUrl":"10.22074/cellj.2023.2009047.1391","url":null,"abstract":"<p><strong>Objective: </strong>Rosuvastatin (RSV) is a hydrophilic, effective statin with a long half-life that stimulates bone regeneration. The present study aims to develop a new scaffold and controlled release system for RSV with favourable properties for bone tissue engineering (BTE).</p><p><strong>Materials and methods: </strong>In this experimental study, high porous polycaprolactone (PCL)-gelatin scaffolds that contained different concentrations of RSV (0 mg/10 ml, 0.1 mg/10 ml, 0.5 mg/10 ml, 2.5 mg/10 ml, 12.5 mg/10 ml, and 62.5 mg/10 ml) were fabricated by the thermally-induced phase separation (TIPS) method. Mechanical and biological properties of the scaffolds were evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), compressive strength, porosity, MTT, alkaline phosphatase (ALP) activity, water contact angle, degradation rate, pH alteration, blood clotting index (BCI), and hemocompatibility.</p><p><strong>Results: </strong>SEM analysis confirmed that the porous structure of the scaffolds contained interconnected pores. FTIR results showed that the RSV structure was maintained during the scaffold's fabrication. RSV (up to 62.5 mg/10 ml) increased compressive strength (16.342 ± 1.79 MPa), wettability (70.2), and degradation rate of the scaffolds. Scaffolds that contained 2.5 mg/10 ml RSV had the best effect on the human umbilical cord mesenchymal stem cell (HUC-MSCs) survival, hemocompatibility, and BCI. As a sustained release system, only 31.68 ± 0.1% of RSV was released from the PCL-Gelatin-2.5 mg/10 ml RSV scaffold over 30 days. In addition, the results of ALP activity showed that RSV increased the osteogenic differentiation potential of the scaffolds.</p><p><strong>Conclusion: </strong>PCL-Gelatin-2.5 mg/10 ml RSV scaffolds have favorable mechanical, physical, and osteogenic properties for bone tissue and provide a favorable release system for RSV. They can mentioned as a a promising strategy for bone regeneration that should be further assessed in animals and clinical studies.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"26 1","pages":"70-80"},"PeriodicalIF":2.0,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10864776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139730815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between Genetic Polymorphism of The lncRNA <i>MIAT</i> rs1894720 with Ischemic Stroke Risk and lncRNA <i>MIAT</i> Expression Levels in The Blood after An Ischemic Stroke: A Case-Control Study.","authors":"Tahereh Asadabadi, Mohammad Javad Mokhtari, Mahnaz Bayat, Anahid Safari, Afshin Borhani-Haghighi","doi":"10.22074/cellj.2023.2003573.1315","DOIUrl":"10.22074/cellj.2023.2003573.1315","url":null,"abstract":"<p><strong>Objective: </strong>Genetic aspects can play an essential role in the occurrence and development of ischemic stroke (IS). Rs1894720 polymorphism is one of the eight single nucleotide polymorphisms (SNPs) in the long non-coding RNA (lncRNA) myocardial infarction-associated transcript (<i>MIAT</i>) locus. The aim of study is the lncRNA <i>MIAT</i> rs1894720 polymorphism decreases IS risk by reducing lncRNA <i>MIAT</i> expression.</p><p><strong>Materials and methods: </strong>In this case-control study, we studied 232 Iranian patients and 232 controls. The blood samples were collected from patients admitted at different times after stroke symptoms. We enrolled 80, 78, and 74 patients who arrived at the hospital between 0-24, 24-48, and 48-72 hours after the first appearance of symptoms, respectively. DNA genotyping was done by the tetra-primer ARMS-PCR method. Circulating MIAT levels were evaluated by real-time polymerase chain reaction (PCR).</p><p><strong>Results: </strong>The GT genotype of <i>MIAT</i> rs1894720 showed a significant association with the risk of IS (OR=3.53, 95% CI=2.13-5.84, P<0.001). <i>MIAT</i> expression was higher relative to the control within the first hours after IS. The <i>MIAT</i> levels in IS patients with rs1894720 (GT) were significantly lower relative to patients who had the GG and TT genotypes. Linear regression model indicated a significant correlation between <i>MIAT</i> expression with atherosclerotic risk factors and types of stroke in IS patients. Receiver operating characteristic (ROC) curve analysis showed that the level of lncRNA <i>MIAT</i> after IS could be diagnostic with an area under the curve (AUC) of 0.82. The sensitivity and specificity were 80.17 and 67.24%, respectively (P<0.001).</p><p><strong>Conclusion: </strong>Our study demonstrated that the <i>MIAT</i> rs1894720 polymorphism (GT) might increase the risk of IS in the Iranian population. <i>MIAT</i> expression was up-regulated in our IS patients. Hence, it could be a diagnostic biomarker for IS.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"25 12","pages":"863-873"},"PeriodicalIF":2.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10777317/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139404896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Evaluation of Collagen-Induced Arthritis in Female Lewis Rats: A Comprehensive Analysis of Disease Progression and Severity.","authors":"Mahnaz Babaahmadi, Nima Makvand Gholipour, Behnoosh Tayebi, Jed Pheneger, Ensiyeh Hajizadeh-Saffar, Mohamadreza Baghaban Eslaminejad, Seyedeh-Nafiseh Hassani","doi":"10.22074/cellj.2023.2004504.1326","DOIUrl":"10.22074/cellj.2023.2004504.1326","url":null,"abstract":"<p><strong>Objective: </strong>The collagen-induced arthritis (CIA) model is the most commonly studied autoimmune model of rheumatoid arthritis (RA). In this study, we investigated the usefulness of collagen type II emulsified in Freund's incomplete adjuvant (CII/IFA) as a suitable method for establishing RA in Lewis rats. The aim of the present study was to present a straightforward and effective method for inducing CIA in rats.</p><p><strong>Materials and methods: </strong>In this experimental study, animals were divided into two equal groups (n=5); control and CIA. Five rats were injected intradermally at the base of the tail with a 0.2 ml CII/IFA emulsion. On the seventh day, a 0.1 ml CII/IFA emulsion booster was injected. Arthritis symptoms that arose were evaluated at clinical, histological, radiological, and at protein expression levels to find out if the disease had been induced successfully.</p><p><strong>Results: </strong>Our finding showed a decreasing trend in the body weight during the RA induction period, while the arthritis score and paw thickness were increased during this period. The results of the enzyme-linked immunosorbent assay (ELISA) for serum samples revealed that the levels of proinflammatory cytokines, interleukin (IL)-1β, IL-6, IL-17, and tumor necrosis factor (TNF)-α and anti-CII IgG were significantly increased in CIA rats compared to the control group. After CIA induction, the level of anti-inflammatory protein IL-10 was decreased significantly. Radiographic examination of the hind paws showed soft tissue swelling, bone erosion, and osteophyte formation in CIA rats. Additionally, based on histological evaluations, the hind paws of the CIA group showed pannus formation, synovial hyperplasia, and bone and cartilage destruction.</p><p><strong>Conclusion: </strong>It seems that CII/IFA treatment can be an appropriate and effective method to induce RA disease in Lewis rats. This well-established and well-characterized CIA model in female Lewis rats could be considered to study aspects of RA and develop novel anti-arthritic agents.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"25 12","pages":"854-862"},"PeriodicalIF":2.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10777318/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139404897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated Bioinformatic Analysis of Differentially Expressed Genes Associated with Wound Healing.","authors":"Mansoureh Farhangniya, Farzaneh Mohamadi Farsani, Najmeh Salehi, Ali Samadikuchaksaraei","doi":"10.22074/cellj.2023.2007217.1368","DOIUrl":"10.22074/cellj.2023.2007217.1368","url":null,"abstract":"<p><strong>Objective: </strong>Wound healing is a complex process involving the coordinated interaction of various genes and molecular<br />pathways. The study aimed to uncover novel therapeutic targets, biomarkers and candidate genes for drug development<br />to improve successful wound repair interventions.<br />Materials and Methods: This study is a network-meta analysis study. Nine wound healing microarray datasets obtained<br />from the Gene Expression Omnibus (GEO) database were used for this study. Differentially expressed genes (DEGs)<br />were described using the Limma package and shared genes were used as input for weighted gene co-expression<br />network analysis. The Gene Ontology analysis was performed using the EnrichR web server, and construction of a<br />protein-protein interaction (PPI) network was achieved by the STRING and Cytoscape.<br />Results: A total of 424 DEGs were determined. A co-expression network was constructed using 7692 shared genes<br />between nine data sets, resulting in the identification of seven modules. Among these modules, those with the top 20<br />genes of up and down-regulation were selected. The top down-regulated genes, including TJP1, SEC61A1, PLEK,<br />ATP5B, PDIA6, PIK3R1, SRGN, SDC2, and RBBP7, and the top up-regulated genes including RPS27A, EEF1A1,<br />HNRNPA1, CTNNB1, POLR2A, CFL1, CSNk1E, HSPD1, FN1, and AURKB, which can potentially serve as therapeutic<br />targets were identified. The KEGG pathway analysis found that the majority of the genes are enriched in the \"Wnt<br />signaling pathway\".<br />Conclusion: In our study of nine wound healing microarray datasets, we identified DEGs and co-expressed modules<br />using WGCNA. These genes are involved in important cellular processes such as transcription, translation, and posttranslational<br />modifications. We found nine down-regulated genes and ten up-regulated genes, which could serve as<br />potential therapeutic targets for further experimental validation. Targeting pathways related to protein synthesis and cell<br />adhesion and migration may enhance wound healing, but additional experimental validation is needed to confirm the<br />effectiveness and safety of targeted interventions.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"25 12","pages":"874-882"},"PeriodicalIF":2.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10777322/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139404899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of Lycopene on Modulating Oxidative Stress and Liver Enzymes Levels in Metabolic Syndrome Patients: A Randomised Clinical Trial.","authors":"Mahdi Mirahmadi, Malihe Aghasizadeh, Fatemeh Nazifkar, Mahla Ghafarian Choubdari, Reza Assaran-Darban, Shima Tavallaie, Hossein Hatamzadeh, Gordon Ferns, Mohammad Reza Mirinezhad, Hamed Baharara, Farzin Hadizadeh, Majid Ghayour-Mobarhan","doi":"10.22074/cellj.2023.2006158.1353","DOIUrl":"10.22074/cellj.2023.2006158.1353","url":null,"abstract":"<p><strong>Objective: </strong>The pathogenesis of metabolic syndrome (MetS) complications involves the excessive production of<br />reactive oxygen species, inflammation, and endothelial dysfunction. Due to Lycopene, a highly unstable structure and<br />its significant effects on modulating the metabolic system, there is a strong need for a formula that can increase its<br />stability. The aim of this study was to develop an approach for encapsulating Lycopene and investigate its effects on<br />inflammatory markers, oxidative stress, and liver enzymes in patients with MetS.<br />Materials and Methods: This study is a simple randomized, double-blind, objective-based clinical trial that involved<br />eighty subjects with MetS, who were equally and randomly assigned to two groups: one group received 20 mg of<br />Lycopene per day for 8 weeks, and the Placebo group followed the same protocol as the Lycopene group but received<br />a placebo instead of Lycopene. They were called Lycopene and placebo, respectively. During follow-up visits after 4<br />and 8 weeks, 20 ml of blood was collected for evaluation of liver enzymes and some inflammatory related markers.<br />Results: Prior to the assignment of volunteers to their respective groups, there were no notable differences in C-reactive<br />protein (CRP), serum liver enzymes, systolic and diastolic blood pressure, or pro-oxidant-antioxidant balance (PAB)<br />between the Lycopene and placebo groups. However, our subsequent analysis revealed a significant reduction in the<br />serum levels of CRP (P=0.001) and PAB (P=0.004) in the group that received Lycopene. Our encapsulated Lycopene<br />treatment was not associated with a significant difference in serum levels of alanine aminotransferase (ALT), aspartate<br />transferase (AST), or alkaline phosphatase (ALP) between our two groups.<br />Conclusion: This study investigated the impact of Lycopene on individuals with MetS, revealing a noteworthy<br />modulation effect on PAB and inflammation linked to MetS. However, no significant differences was demonstrated in<br />serum levels of ALT, AST and ALP between the studied group (registration number: IRCT20130507013263N3).</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"25 12","pages":"847-853"},"PeriodicalIF":2.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10777315/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139404925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Left Barrel Cortical Neurons Activity following Transplantation of Stem Cells into Right Lesioned-Barrel Cortex in Rats.","authors":"Mansoureh Sabzalizadeh, Mohammad Reza Afarinesh, Ali Derakhshani, Vahid Sheibani","doi":"10.22074/cellj.2023.2007586.1373","DOIUrl":"10.22074/cellj.2023.2007586.1373","url":null,"abstract":"<p><strong>Objective: </strong>Stem cells (SCs) can improve the functional defects of brain injury. Rodents use their whiskers to get tactile information from their surroundings. The aim of this study was to investigate whether the transplantation of SCs into the lesioned barrel cortex can help neuronal function in the contralateral cortex.</p><p><strong>Materials and methods: </strong>Sixteen male Wistar rats (200-230 g) were used in this experimental study. We induced a mechanical lesion in the right barrel cortex area of rats by removing this area by a 3 mm skin punch. Four groups containing one intact group of rats: group 1: control, and three lesion groups, group 2: lesion+un-differentiated dental pulp SCs (U-DPSCs), group 3: lesion+differentiated dental pulp SCs (D-DPSCs), and group 4: cell medium (vehicle) that were injected in the lesion area. Three weeks after transplantation of SCs or cell medium, the rats' responses of left barrel cortical neurons to controlled deflections of right whiskers were recorded by using the extracellular single-unit recordings technique.</p><p><strong>Results: </strong>The results showed that the neural spontaneous activity and response magnitude of intact barrel cortex neurons in the lesion group decreased significantly (P<0.05) compared to the control group while ON and OFF responses were improved in the D-DPSCs (P<0.001) group compared to the vehicle group three weeks after transplantation.</p><p><strong>Conclusion: </strong>Transplantation of dental pulp mesenchymal SCs significantly improved the neural responses of the left barrel cortex that was depressed in the vehicle group.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"25 12","pages":"822-828"},"PeriodicalIF":2.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10777320/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139404900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering Role of lncRNA 91H in Liver Cancer: Impact on Tumorigenesis.","authors":"Zhiyuan Mo, Zhuangqiang Wang","doi":"10.22074/cellj.2023.2010456.1395","DOIUrl":"10.22074/cellj.2023.2010456.1395","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate functional role of long ncRNA (lncRNA) 91H in liver cancer tumorigenesis, focusing on its effect on cell proliferation, apoptosis, cell cycle progression, migration, invasion, epithelial-mesenchymal transition (EMT) and <i>In vivo</i> tumor growth.</p><p><strong>Materials and methods: </strong>In this experimental study, liver cancer tissues and cell lines were analyzed for lncRNA 91H expression using quantitative reverse transcription polymerase chain reaction (qRT-PCR). By employing si-RNA to silence 91H, we aimed to gain a more in-depth understanding of its specific contributions and effects within these cells. Cell proliferation was assessed through the CCK-8 assay, while apoptosis and cell cycle progression were quantified using Annexin V-FITC staining and flow cytometry, respectively. Migration and invasion capabilities of liver cancer cells were assessed through transwell assay. EMT was assessed by analyzing protein expression levels of EMT-associated markers through western blotting. <i>In vivo</i> effect of 91H was assessed through xenograft experiments.</p><p><strong>Results: </strong>Significantly higher levels of lncRNA 91H were observed in the liver cancer tissues and cell lines, than the normal cells. Silencing 91H in liver cancer cells led to a notable reduction of cell proliferation by inducing apoptosis and arresting the cell cycle. Liver cancer cells with decreased 91H expression exhibited diminished migration and invasion abilities, suggesting a role for 91H in promoting these processes. Furthermore, 91H knockdown weakened EMT in liver cancer cells, indicating its involvement in modulating this critical cellular transition. Furthermore, growth of subcutaneous xenograft tumors and weight was effectively suppressed by sh-lncRNA 91H.</p><p><strong>Conclusion: </strong>Our study strongly supports lncRNA 91H's role in liver cancer progression by enhancing proliferation, migration, invasion, and EMT. Targeting 91H reduced in vivo tumor growth, highlighting its potential as a therapeutic liver cancer target. These findings suggest 91H's pivotal role in liver cancer aggressiveness, opening doors for future therapeutic approaches.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"25 12","pages":"829-838"},"PeriodicalIF":2.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10777316/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139404898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SP-8356: A Novel Verbenone Derivative Exerts <i>In Vitro</i> Anti-Non-Small Cell Lung Cancer Effects, Promotes Apoptosis via The P53/MDM2 Axis and Inhibits Tumor Formation in Mice.","authors":"Lei Yang, Liyi Hu","doi":"10.22074/cellj.2023.2008708.1385","DOIUrl":"10.22074/cellj.2023.2008708.1385","url":null,"abstract":"<p><strong>Objective: </strong>Non-small cell lung cancer (NSCLC) stands as a prominent contributor to cancer-related fatalities on a global scale, necessitating the search for novel therapeutic agents. SP-8356, a derivative of (1S)-(-)-verbenone, has shown promise as an anticancer agent in preclinical studies. However, specific mechanisms underlying its effects in NSCLC remain to be elucidated. The aim of this research was to explore the in vitro anti-NSCLC effects of SP-8356, elucidate its mechanisms of action, and assess its efficacy in inhibiting tumor formation in a murine model.</p><p><strong>Materials and methods: </strong>In this experimental study, NSCLC cell lines were treated with various concentrations of SP- 8356. Cell viability and proliferation were assessed using MTT and colony formation assays, respectively. Cell cycle distribution was analyzed by flow cytometry, and apoptosis was evaluated by determining apoptotic protein expression. Western blot analysis was conducted to assess protein expression levels of the both p53 and MDM2. Additionally, we evaluated efficacy of the SP-8356 in inhibiting tumor formation of the nude mouse model.</p><p><strong>Results: </strong>SP-8356 demonstrated a concentration-dependent inhibition of cell proliferation in the NSCLC cell lines. Flow cytometric analysis showed that SP-8356 led to cell cycle arrest at the G2/M phase, indicating its potential influence on regulating the cell cycle. SP-8356 treatment was associated with the downregulation of CDK1 and Cyclin B1. Additionally, SP-8356 significantly enhanced apoptosis in NSCLC cells. SP-8356 treatment was associated with the downregulation of Bcl-2, while Bax expression was upregulated. Mechanistically, SP-8356 led to accumulation of the p53 protein levels within the NSCLC cells. This accumulation was mediated through inhibition of its negative regulator, MDM2. Using a nude mouse model demonstrated that SP-8356 effectively inhibited tumor formation in vivo.</p><p><strong>Conclusion: </strong>Our findings shed light on the molecular mechanisms underlying anticancer activity of SP-8356 and highlight its potential as a promising therapeutic candidate for NSCLC treatment.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"25 12","pages":"839-846"},"PeriodicalIF":2.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10777321/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139404913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Biomaterials on Human Dental Pulp Stem Cell Neural Differentiation: A Scoping Review.","authors":"Maedeh Khatami, Yousef Moradi, Ramyar Rahimi Darehbagh, Donya Azizi, Arash Pooladi, Rojin Ramezani, Seyedeh Asrin Seyedoshohadaei","doi":"10.22074/cellj.2023.2007711.1375","DOIUrl":"10.22074/cellj.2023.2007711.1375","url":null,"abstract":"<p><p>Neural cells are the most important components of the nervous system and have the duty of electrical signal transmission. Damage to these cells can lead to neurological disorders. Scientists have discovered different methods, such as stem cell therapy, to heal or regenerate damaged neural cells. Dental stem cells are among the different cells used in this method. This review attempts to evaluate the effect of biomaterials mentioned in the cited papers on differentiation of human dental pulp stem cells (hDPSCs) into neural cells for use in stem cell therapy of neurological disorders. We searched international databases for articles about the effect of biomaterials on neuronal differentiation of hDPSCs. The relevant articles were screened by title, abstract, and full text, followed by selection and data extraction. Totally, we identified 731 articles and chose 18 for inclusion in the study. A total of four studies employed polymeric scaffolds, four assessed chitosan scaffolds (CS), two utilised hydrogel scaffolds, one investigation utilised decellularised extracellular matrix (ECM), and six studies applied the floating sphere technique. hDPSCs could heal nerve damage in regenerative medicine. In the third iteration of nerve conduits, scaffolds, stem cells, regulated growth factor release, and ECM proteins restore major nerve damage. hDPSCs must differentiate into neural cells or neuron-like cells to regenerate nerves. Plastic-adherent cultures, floating dentosphere cultures, CS, polymeric scaffolds, hydrogels, and ECM mimics have been used to differentiate hDPSCs. According to our findings, the floating dentosphere technique and 3D-PLAS are currently the two best techniques since they result in neuroprogenitor cells, which are the starting point of differentiation and they can turn into any desired neural cell.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"25 12","pages":"813-821"},"PeriodicalIF":1.7,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10777319/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139404924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Osteoblastic Differentiation of Stem Cells from Human Exfoliated Deciduous Teeth by Probiotic Hydroxyapatite.","authors":"Sabere Nouri, Rasoul Roghanian, Giti Emtiazi, Oguzhan Gunduz, Rasoul Shafiei","doi":"10.22074/cellj.2023.1999743.1276","DOIUrl":"https://doi.org/10.22074/cellj.2023.1999743.1276","url":null,"abstract":"<p><strong>Objective: </strong>Multipotent cells derived from human exfoliated deciduous teeth (SHED) possess the ability to differentiate into various cell types, including osteoblasts. This study aims to simulate the growth induction and osteogenic differentiation of SHED cells using probiotics and their resultant biomaterials.</p><p><strong>Materials and methods: </strong>This experimental study proceeded in two stages. Initially, we evaluated the effect of autoclaved nutrient agar (NA) grown probiotic <i>Bacillus coagulans</i> (<i>B. coagulans)</i> on the SHED and MG-63 cell lines. Subsequently, probiotics grown on the Pikovskaya plus urea (PVKU) medium and their synthesised hydroxyapatite (HA) were identified using X-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray (EDX), and Fourier transform infrared spectroscopy (FTIR), and then used to stimulate growth and osteogenic differentiation of the SHED cell line. Osteoblast cell differentiation was assessed by morphological changes, the alkaline phosphatase (ALP) assay, and alizarin red staining.</p><p><strong>Results: </strong>There was a substantial increase in SHED cell growth of about 14 and 33% due to probiotics grown on NA and PVKU medium, respectively. The PVKU grown probiotics enhanced growth and induced stem cell differentiation due to HA content. Evidence of this differentiation was seen in the morphological shift from spindle to osteocyte-shaped cells after five days of incubation, an increase in ALP level over 21 days, and detection of intracellular calcium deposits through alizarin red staining-all indicative of osteoblast cell development.</p><p><strong>Conclusion: </strong>The osteogenic differentiation process in stem cells, improved by the nano-HA-containing byproducts of probiotic bacteria in the PVKU medium, represents a promising pathway for leveraging beneficial bacteria and their synthesised biomaterials in tissue engineering.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"25 11","pages":"753-763"},"PeriodicalIF":2.0,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10711290/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138812006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}