Cell Journal最新文献

{"title":"Umbilical Cord Blood-Derived Monocytes as A Reliable Source of Functional Macrophages for Biomedical Research.","authors":"Shukoofeh Torabi,&nbsp;Morteza Zarrabi,&nbsp;Nikoo Hossein-Khannazer,&nbsp;Majid Lotfinia,&nbsp;Masoumeh Nouri,&nbsp;Roberto Gramignoli,&nbsp;Moustapha Hassan,&nbsp;Massoud Vosough","doi":"10.22074/cellj.2023.1990203.1238","DOIUrl":"10.22074/cellj.2023.1990203.1238","url":null,"abstract":"<p><strong>Objective: </strong>Macrophages are multifunctional immune cells widely used in immunological research. While autologous macrophages have been widely used in several biomedical applications, allogeneic macrophages have also demonstrated similar or even superior therapeutic potential. The umbilical cord blood (UCB) is a well-described source of abundant allogenic monocytes and macrophages that is easy to collect and can be processed without invasive methods. Current monocyte isolation procedures frequently result in heterogenous cell products, with limited yields, activated cells, and high cost. This study outlines a simple isolation method that results in high yields and pure monocytes with the potential to differentiate into functional macrophages.</p><p><strong>Materials and methods: </strong>In the experimental study, we describe a simple and efficient protocol to isolate highpurity monocytes. After collection of human UCB samples, we used a gradient-based procedure composed of three consecutive gradient steps: i. Hydroxyethyl starch-based erythrocytes sedimentation, followed by ii. Mononuclear cells (MNCs) isolation by Ficoll-Hypaque gradient, and iii. Separation of monocytes from lymphocytes by a slight hyperosmolar Percoll gradient (0.573 g/ml). Then the differentiation potential of isolated monocytes to pro- and antiinflammatory macrophages were evaluated in the presence of granulocyte colony-stimulating factor (GM-CSF) and macrophage CSF (M-CSF), respectively. The macrophages were functionally characterized as well.</p><p><strong>Results: </strong>A high yield of monocytes after isolation (25 to 50 million) with a high purity (>95%) could be obtained from every 100-150 ml UCB. Isolated monocytes were defined based on their phenotype and surface markers expression pattern. Moreover, they possess the ability to differentiate into pro- or anti-inflammatory macrophages with specific phenotypes, gene/surface protein markers, cytokine secretion patterns, T-cell interactions, and phagocytosis activity.</p><p><strong>Conclusion: </strong>Here we describe a simple and reproducible procedure for isolation of pure monocytes from UCB, which could be utilized to provide functional macrophages as a reliable and feasible source of allogenic macrophages for biomedical research.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/11/fd/Cell-J-25-524.PMC10542205.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10484862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-Dimensional Imaging and Quantitative Analysis of Blood Vessel Distribution in The Meniscus of Transgenic Mouse after Tissue Clearing.","authors":"Huaixuan Sheng,&nbsp;Mingru Huang,&nbsp;Huizhu Li,&nbsp;Luyi Sun,&nbsp;Sijia Feng,&nbsp;Xiner Du,&nbsp;Yicong Wang,&nbsp;Xiaoyu Tong,&nbsp;Yi Feng,&nbsp;Jun Chen,&nbsp;Yunxia Li","doi":"10.22074/cellj.2023.1988973.1220","DOIUrl":"10.22074/cellj.2023.1988973.1220","url":null,"abstract":"<p><strong>Objective: </strong>Blood supply to the meniscus determines its recovery and is a reference for treatment planning. This study aimed to apply tissue clearing and three-dimensional (3D) imaging in exploring the quantitative distribution of blood vessels in the mouse meniscus.</p><p><strong>Materials and methods: </strong>In this experimental study, tissue clearing was performed to treat the bilateral knee joints of transgenic mice with fluorescent vascular endothelial cells. Images were acquired using a light sheet microscope and the vascular endothelial cells in the meniscus was analysed using 3D imaging. Quantitative methods were employed to further analyse the blood vessel distribution in the mouse meniscus.</p><p><strong>Results: </strong>The traditional three-equal-width division of the meniscus is as follows: the outer one-third is the red-red zone (RR), the inner one-third is the white-white zone (WW), and the transition area is the red-white zone (RW). The division revealed significant signal differences between the RW and WW (P<0.05) zones, but no significant differences between the RR and RW zones, which indicated that the division might not accurately reflect the blood supply of the meniscus. According to the modified division (4:2:1) in which significant differences were ensured between the adjacent zones, we observed that the width ratio of each zone was 38 ± 1% (RR), 24 ± 1% (RW), and 38 ± 2% (WW). Furthermore, the blood supply to each region was verified. The anterior region had the most abundant blood supply. The fluorescence count in the anterior region was significantly higher than in the central and posterior regions (P<0.05). The blood supply of the medial meniscus was superior to the lateral meniscus (P<0.05).</p><p><strong>Conclusion: </strong>Analysis of the blood supply to the mouse meniscus under tissue clearing and 3D imaging reflect quantitative blood vessel distribution, which would facilitate future evaluations of the human meniscus and provide more anatomical references for clinicians.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/75/a0/Cell-J-25-570.PMC10542206.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10466737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regularized Machine Learning Models for Prediction of Metabolic Syndrome Using <i>GCKR, APOA5,</i> and <i>BUD13</i> Gene Variants: Tehran Cardiometabolic Genetic Study.","authors":"Nadia Alipour,&nbsp;Anoshirvan Kazemnejad,&nbsp;Mahdi Akbarzadeh,&nbsp;Farzad Eskandari,&nbsp;Asiyeh Sadat Zahedi,&nbsp;Maryam S Daneshpour","doi":"10.22074/cellj.2023.2000864.1294","DOIUrl":"10.22074/cellj.2023.2000864.1294","url":null,"abstract":"<p><strong>Objective: </strong>Metabolic syndrome (MetS) is a complex multifactorial disorder that considerably burdens healthcare systems. We aim to classify MetS using regularized machine learning models in the presence of the risk variants of <i>GCKR, BUD13</i> and <i>APOA5</i>, and environmental risk factors.</p><p><strong>Materials and methods: </strong>A cohort study was conducted on 2,346 cases and 2,203 controls from eligible Tehran Cardiometabolic Genetic Study (TCGS) participants whose data were collected from 1999 to 2017. We used different regularization approaches [least absolute shrinkage and selection operator (LASSO), ridge regression (RR), elasticnet (ENET), adaptive LASSO (aLASSO), and adaptive ENET (aENET)] and a classical logistic regression (LR) model to classify MetS and select influential variables that predict MetS. Demographics, clinical features, and common polymorphisms in the <i>GCKR, BUD13</i> and <i>APOA5</i> genes of eligible participants were assessed to classify TCGS participant status in MetS development. The models' performance was evaluated by 10-repeated 10-fold crossvalidation. Various assessment measures of sensitivity, specificity, classification accuracy, and area under the receiver operating characteristic curve (AUC-ROC) and AUC-precision-recall (AUC-PR) curves were used to compare the models.</p><p><strong>Results: </strong>During the follow-up period, 50.38% of participants developed MetS. The groups were not similar in terms of baseline characteristics and risk variants. MetS was significantly associated with age, gender, schooling years, body mass index (BMI), and alternate alleles in all the risk variants, as indicated by LR. A comparison of accuracy, AUCROC, and AUC-PR metrics indicated that the regularization models outperformed LR. Regularized machine learning models provided comparable classification performances, whereas the aLASSO model was more parsimonious and selected fewer predictors.</p><p><strong>Conclusion: </strong>Regularized machine learning models provided more accurate and parsimonious MetS classifying models. These high-performing diagnostic models can lay the foundation for clinical decision support tools that use genetic and demographical variables to locate individuals at high risk for MetS.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2a/7b/Cell-J-25-536.PMC10542204.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10466733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-Culture of Mouse Blastocysts on A Human Recellularized Endometrial Scaffold: An <i>In Vitro</i> Model for Future Implantation Studies.","authors":"Elham Sadeghi,&nbsp;Mojtaba Rezazadeh Valojerdi,&nbsp;Mojdeh Salehnia","doi":"10.22074/cellj.2023.1989926.1236","DOIUrl":"10.22074/cellj.2023.1989926.1236","url":null,"abstract":"<p><strong>Objective: </strong>This study evaluates the interaction of mouse blastocysts as a surrogate embryo on a recellularized endometrial scaffold by seeding human endometrial mesenchymal cells (hEMCs).</p><p><strong>Materials and methods: </strong>In this experimental study, prepared decellularized human endometrial tissues were characterized by morphological staining, DNA content analysis, and scanning electron microscopic (SEM) analysis. The scaffolds were subsequently recellularized by hEMCs. After seven days of cultivation, the mouse blastocysts were co-cultured on the recellularized scaffolds for 48 hours. Embryo attachment and implantation within these scaffolds were evaluated at the morphological, ultrastructural, molecular, and hormonal levels.</p><p><strong>Results: </strong>There was no morphological evidence of cells and nuclei in the decellularized scaffold. DNA content significantly decreased by 89.92% compared to the control group (P<0.05). Both decellularized and native tissues had similar patterns of collagen bundles and elastin fibers, and glycosaminoglycan (GAGs) distribution in the stroma. After recellularization, the hEMCs attached to the scaffold surface and penetrated different parts of these scaffolds. In the co-cultured group, the embryo attached to the surface of the scaffold after 24 hours and penetrated the recellularized endometrial tissue after 48 hours. We observed multi-layered organoid-like structures formed by hEMC proliferation. The relative expressions of epithelial-related genes, <i>ZO-1</i> and <i>COL4A1</i>, and <i>SSP1, MMP2,</i> and <i>PRL</i>, as decidualizationrelated genes, were significantly higher in the recellularized group on day 9 in the presence of the embryo compared to the other groups (P<0.05). Beta human chorionic gonadotropin (β-hCG) and prolactin were statistically increased in the recellularized group on day 9 group (P<0.05).</p><p><strong>Conclusion: </strong>hEMCs and mouse embryo co-cultured on a decellularized endometrial scaffold provides an alternative model to study embryo implantation and the earlier stage of embryo development.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b2/0b/Cell-J-25-579.PMC10542203.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10466735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increased Apoptosis in Subcortical Regions of The Visual Pathway in Offspring Born to Diabetic Rats.","authors":"Nasim Alipour,&nbsp;Somaye Fallahnezhad,&nbsp;Javad Bagheri,&nbsp;Hamideh Babaloo,&nbsp;Fatemeh Tahmasebi,&nbsp;Ghasem Sazegar,&nbsp;Hossein Haghir","doi":"10.22074/cellj.2023.1989649.1232","DOIUrl":"10.22074/cellj.2023.1989649.1232","url":null,"abstract":"<p><strong>Objective: </strong>Diabetes in pregnancy is a prevalent disease that can affect the central nervous system of the fetus by hyperglycemia. This study aimed to investigate the impact of maternal diabetes on neuronal apoptosis in the superior colliculus (SC) and the lateral geniculate nucleus (LGN) in male neonates born to diabetic mothers.</p><p><strong>Materials and methods: </strong>In this experimental study, female adult rats were separated into three groups: control, diabetic (induced using an intraperitoneal injection of streptozotocin), and insulin-treated diabetic [diabetes controlled by subcutaneous neutral protamine hagedorn (NPH)-insulin injection]. Male neonates from each group were euthanized on 0, 7, and 14 postnatal days (P0, P7, and P14, respectively), and apoptotic cells were identified using TUNEL staining.</p><p><strong>Results: </strong>The numerical density per unit area (NA) of apoptotic cells was significantly higher in SC and the dorsal LGN (dLGN) in neonates born to the diabetic rats compared to the control group at P0, P7, and P14. However, insulin treatment normalized the number of apoptotic cells.</p><p><strong>Conclusion: </strong>This study demonstrated that maternal diabetes increased apoptosis in dLGN and SC of male neonates at P0, P7, and P14.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6e/e1/Cell-J-25-564.PMC10542209.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10466734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oleuropein as An Effective Suppressor of Inflammation and MicroRNA-146a Expression in Patients with Rheumatoid Arthritis.","authors":"Hanieh Mahi,&nbsp;Zahra Yousefi,&nbsp;Fatemeh Toufan,&nbsp;Maryam Yarmohammadi,&nbsp;Moslem Jafarisani,&nbsp;Nahid Eskandari,&nbsp;Mohammad-Hossein Shams,&nbsp;Reza Jafari","doi":"10.22074/cellj.2023.1989941.1237","DOIUrl":"https://doi.org/10.22074/cellj.2023.1989941.1237","url":null,"abstract":"<p><strong>Objective: </strong>Rheumatoid arthritis (RA) is a common progressive autoimmune disorder that causes chronic inflammation of the joints and damage to other organs. Previous studies have reported the important role of miRNA-146a in the pathogenesis of RA. In addition, the anti-inflammatory and modulatory effects of oleuropein (OLEU) on the expression pattern of microRNAs (miRNAs) have been shown in different diseases. Therefore, this study aimed to evaluate both the sensitivity and specificity of miRNA-146a and determine the potential effects of OLEU on the expression levels of miRNA-146a and tumour necrosis factor-alpha (TNF-α) in RA patients.</p><p><strong>Materials and methods: </strong>The participants in this experimental study were divided into 2 groups: RA (n=45) and healthy controls (n=30). The isolated peripheral blood mononuclear cells (PBMCs) were treated with different concentrations of OLEU; and the level of TNF-α expression, anti-citrullinated protein, and miRNA-146a were determined using enzyme-linked immunoassay and real-time polymerase chain reaction, respectively. In addition, the receiver operating characteristic (ROC) curve analysis evaluated the sensitivity and specificity of miRNA-146a in RA patients.</p><p><strong>Results: </strong>Results revealed a positive correlation between the levels of miRNA-146a expression with the serum levels of C-reactive protein (CRP) and rheumatoid factor (RF) in RA patients. In addition, OLEU treatment decreased the levels of TNF-α and miRNA-146a expression in treated PBMCs samples compared with untreated cells. The ROC curve analysis showed an 85% sensitivity and 100% specificity of miRNA-146a in RA patients.</p><p><strong>Conclusion: </strong>Therefore, miRNA-146a can be used as a useful biomarker for RA diagnosis, particularly for early detection. In addition, OLEU could suppress inflammation in RA patients through the regulation of miRNA-146a.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a6/ba/Cell-J-25-505.PMC10404353.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9942856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of The Conditioned Medium from Human Embryonic Stem Cells on Mouse Oocytes In Vitro Maturation.","authors":"Roshan Rezaee Ranjbar Sardari,&nbsp;Mahmood Dehghani Ashkezari,&nbsp;Seyed Morteza Seifati,&nbsp;Mahin Izadi,&nbsp;Jalal Golzadeh,&nbsp;Fahime Mazaheri,&nbsp;Maryam Adib,&nbsp;Behrouz Aflatoonian","doi":"10.22074/cellj.2023.1987024.1217","DOIUrl":"https://doi.org/10.22074/cellj.2023.1987024.1217","url":null,"abstract":"<p><strong>Objective: </strong>Some reports have indicated that conditioned medium from growing mouse embryonic stem cells (ESCs) provides a supportive condition for small follicles growing, oocyte maturation, and following embryo growth. The aim of this study is assessing in vitro maturation (IVM) and consequent <i>in vitro</i> fertilization (IVF) outcome of immature mouse oocytes using human embryonic stem cells conditioned medium (HESCM).</p><p><strong>Materials and methods: </strong>In this experimental study, 240 germinal vesicle (GV) oocytes were took from NMRI female mice, aged 4-6 weeks, 48 hours before injection of 5 IU pregnant mare serum gonadotropin (PMSG). 120 GV oocytes without cumulus cells were cultured in each of the groups. 120 GV were cultured in HESCM as test groups and also 120 GV cultured in human embryonic stem cells medium (HESM) as control groups. After evaluating the metaphase II (MII) oocyte maturation rate at 8, 16 and 24 hours, the MII oocytes subsequently were fertilized <i>in vitro</i> and the two-cell embryo development rate was recorded at days 1, 2, and 3. Statistical analysis was performed by using the generalized estimating equations (GEE) method that calculated their rate ratio.</p><p><strong>Results: </strong>Our data indicated there are significant differences between the maturation rates in HESCM and HESM (P=0.004), also the two-cell embryo development was significant between two culture media (P=0.00).</p><p><strong>Conclusion: </strong>Similar to some other studies, the secretome of the HESCM showed a significant impact on the IVM outcomes in mice.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/56/26/Cell-J-25-455.PMC10404354.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9949026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mouse Embryonic Fibroblasts-Derived Extracellular Matrix Facilitates Expansion of Inner Ear-Derived Cells.","authors":"Junming Zhang,&nbsp;Li Liu,&nbsp;Yuexia Li,&nbsp;Jinglei Wu,&nbsp;Xiangxin Lou","doi":"10.22074/cellj.2023.1989426.1228","DOIUrl":"https://doi.org/10.22074/cellj.2023.1989426.1228","url":null,"abstract":"<p><strong>Objective: </strong>Previous reports showed that mouse embryonic fibroblasts (MEFs) could support pluripotent stem cell selfrenewal and maintain their pluripotency. The goal of this study was to reveal whether the decellularized extracellular matrix derived from MEFs (MEF-ECM) is beneficial to promote the proliferation of inner ear-derived cells.</p><p><strong>Materials and methods: </strong>In this experimental study, we prepared a cell-free MEF-ECM through decellularization. Scanning electron microscope (SEM) and immunofluorescent staining were conducted for phenotype characterization. Organs of Corti were dissected from postnatal day 2 and the inner ear-derived cells were obtained. The identification of inner ear-derived cells was conducted by using reverse transcription-polymerase chain reaction (RT-PCR). Cell counting kit-8 (CCK-8) was used to evaluate the proliferation capability of inner ear-derived cells cultured on the MEFECM and tissue culture plate (TCP).</p><p><strong>Results: </strong>The MEF-ECM was clearly observed after decellularization via SEM, and the immunofluorescence staining results revealed that MEF-ECM was composed of three proteins, including collagen I, fibronectin and laminin. Most importantly, the results of CCK-8 showed that compared with TCP, MEF-ECM could effectively facilitate the proliferation of inner ear-derived cells.</p><p><strong>Conclusion: </strong>The discovery of the potential of MEF-ECM in promoting inner ear-derived cell proliferation indicates that the decellularized matrix microenvironment may play a vital role in keeping proliferation ability of these cells. Our findings indicate that the use of MEF-ECM may serve as a novel approach for expanding inner ear-derived cells and potentially facilitating the clinical application of inner ear-derived cells for hearing loss in the future.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b0/e5/Cell-J-25-447.PMC10404357.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9942855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic Effects of Capsaicin and Quercetin Improved Induced Premature Ovarian Failure in Rat.","authors":"Saman Moradi,&nbsp;Mozafar Khazaei,&nbsp;Zahra Rashidi","doi":"10.22074/cellj.2023.1989732.1234","DOIUrl":"https://doi.org/10.22074/cellj.2023.1989732.1234","url":null,"abstract":"<p><strong>Objective: </strong>Premature ovarian failure (POF) is a heterogeneous disorder. POF is defined as hypergonadotropic hypoestrogenism in women under 40 years. There is no effective treatment to cure POF patients. Antioxidants prevent ovarian damage by reducing the lipid peroxidation cascades affecting folliculogenesis, meiosis and ovulation. Hence; the aim of present study was to investigate the effects of Capsaicin (CAP) and Quercetin (QUR) on cyclophosphamide (CYC)-induced POF in rat model.</p><p><strong>Materials and methods: </strong>In this experimental study, POF was induced by intraperitoneal injection of 200 mg/kg CYC on first day and then 8 mg/kg/day for the following 3 days. After 4 days of CYC administration, rats were randomly divided into five groups (n=6/group) as follows: POF, dimethyl sulfoxide (DMSO), CAP (0.5 mg/kg/day), QUR (100 mg/kg/day) and CAP+QUR. Biochemical, hormonal, gene expression, and histological evaluations were performed on blood serum and tissue samples after 14 days of treatment with the CAP and QUR.</p><p><strong>Results: </strong>CAP, QUR and CAP+QUR groups showed signs of restored ovarian function in the form of a significant increase in serum total antioxidant capacity (TAC), estrogen, progesterone and anti-mullerian hormone (AMH) levels versus POF and DMSO groups and a significant improvement in histological parameters and follicle numbers in treatment groups compared to POF and DMSO groups. Polymerase chain reaction (PCR) analysis demonstrated that CAP and QUR upregulate the expression of BAX gene and decreased the expression of apoptosis inducing genes (BCL-2 and P53).</p><p><strong>Conclusion: </strong>CAP and QUR treatment of CYC-induced POF rats showed a positive effect on reducing ovarian damage by improving TAC levels, expression of apoptotic genes, levels of ovarian reserve markers, and histological parameters. Our results suggest that treatment with CAP or QUR may be a conservative treatment approach for CYC -induced POF.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/48/16/Cell-J-25-496.PMC10404358.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10323857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Training Type on The Signaling P athway of Ceramide-Dependent Insulin Resistance in The Flexor Hallucis Longus Muscle of Streptozotocin-Induced Diabetic Rats.","authors":"Pakhshan Rahimpour,&nbsp;Dariush Sheikholeslami-Vatani,&nbsp;Mohammad Raman Moloudi,&nbsp;Saeed Ghaeeni","doi":"10.22074/cellj.2023.1971874.1158","DOIUrl":"https://doi.org/10.22074/cellj.2023.1971874.1158","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to compare the effect of different physical training on the mechanism of ceramidedependent insulin resistance in the flexor hallucis longus (FHL) muscle of diabetic rats.</p><p><strong>Materials and methods: </strong>In this experimental study, 7 healthy as a healthy control (HC) group, and 21 diabetics (55 mg/ kg Streptozotocin) Wistar rats (200-220 g; 8-10 weeks old) divided into the diabetic control (DC), moderate continuous training (MCT), and moderate intensity interval training (MIIT) groups. Both MCT (55-70% of maximal oxygen uptake (VO_2max), and MIIT (85% VO_2max) groups trained for 10-25 minutes at a speed of 10-20 m/minutes. The changes in the expression of blood glucose, insulin, insulin resistance, lipid profile and total ceramide were measured as well as ceramide synthase-1, Glucose transporter type 4 (GLUT4), Protein kinase B known as Akt, phosphorylated protein kinase B known as pAkt, protein kinase C (PKC), and tumour necrosis factor α (TNFα).</p><p><strong>Results: </strong>Blood glucose, triglyceride (TG) and ceramide synthase-1 (CS1) expression levels in the MCT group decreased in comparison with the DC group. FHL protein expression of GLUT4 in the MCT group was higher than the DC group. FHL expression of GLUT4, pAKT, AKT/pAKT, PKC, CS1 and total ceramide in the MIIT group were higher than the DC group. Cholesterol, low-density lipoprotein (LDL), TG, and TNF-α protein expression in the MIIT group were lower than the DC group. GLUT4, PKC, pAKT, AKT/pAKT in the MIIT group were higher, and total ceramide and TNF-α were lower in the MIIT group than the MCT group.</p><p><strong>Conclusion: </strong>It seems that both training plan MIIT and MCT have favorable effects on the metabolism of glucose, insulin, lipids, and the decrease of TNFα level in the diabetes, but in connection with the improvement of the ceramides mechanism, it seems that the MIIT training plan is more optimal than MCT training plan.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/eb/cf/Cell-J-25-461.PMC10404356.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9951529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}