Bioinformatic Analysis of The Prognostic Value of A Panel of Six Amino Acid Transporters in Human Cancers.

IF 1.7 4区生物学 Q4 CELL BIOLOGY

Cell Journal Pub Date : 2023-09-09 DOI:10.22074/cellj.2023.2004011.1319

Yaqi Liu, Haijuan Xiong, Chenhui Yan, Yalei Wang, Wenfeng Cao, Shuo Qie

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引用次数: 0

Abstract

Objective: Solid tumor cells utilize amino acid transporters (AATs) to increase amino acid uptake in response to nutrient-insufficiency. The upregulation of AATs is therefore critical for tumor development and progression. This study identifies the upregulated AATs under amino acid deprived conditions, and further determines the clinicopathological importance of these AATs in evaluating the prognosis of patients with cancers.

Materials and methods: In this experimental study, the Gene Expression Omnibus (GEO) datasets (GSE62673, GSE26370, GSE125782 and GSE150874) were downloaded from the NCBI website and utilized for integrated differential expression and pathway analysis v0.96, Gene Set Enrichment Analysis (GSEA), and REACTOME analyses to identify the AATs upregulated in response to amino acid deprivation. In addition, The Cancer Genome Atlas (TCGA) datasets with prognostic information were assessed and employed to evaluate the association of identified AATs with patients' prognoses using SurvExpress analysis.

Results: Using analysis of NCBI GEO data, this study shows that amino acid deprivation leads to the upregulation of six AAT genes; SLC3A2, SLC7A5, SLC7A1, SLC1A4, SLC7A11 and SLC1A5. GSEA and REACTOME analyses identified altered signaling in cells exposed to amino acid deprivation, such as pathways related to stress responses, the cell cycle and apoptosis. In addition, Principal Component Analysis showed these six AAT genes to be well divided into two distinct clusters in relation to TCGA tumor tissues versus normal counterparts. Finally, Log-Rank analysis confirmed the upregulation of this panel of six AAT genes is correlated with poor prognosis in patients with colorectal, esophageal, kidney and lung cancers.

Conclusion: The upregulation of a panel of six AATs is common in several human cancers and may provide a valuable diagnostic tool to evaluate the prognosis of patients with colorectal, esophageal, kidney and lung cancers.

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六种氨基酸转运蛋白对人类癌症预后价值的生物信息学分析。

目的：实体瘤细胞利用氨基酸转运蛋白（AATs）增加氨基酸摄取以应对营养不足。因此，AATs的上调对肿瘤的发展和进展至关重要。本研究确定了氨基酸缺乏条件下上调的AAT，并进一步确定了这些AAT在评估癌症患者预后中的临床病理重要性。材料和方法：在本实验研究中，从NCBI网站下载基因表达综合（GEO）数据集（GSE62673、GSE26370、GSE125782和GSE150874），并用于整合差异表达和通路分析v0.96、基因集富集分析（GSEA）和REACTOME分析，以确定在氨基酸剥夺反应中上调的AATs。此外，对癌症基因组图谱（TCGA）数据集和预后信息进行了评估，并使用SurvExpress分析来评估已识别AAT与患者预后的关联。结果：通过对NCBI GEO数据的分析，本研究表明氨基酸剥夺导致6个AAT基因的上调；SLC3A2、SLC7A5、SLC7A1、SLC1A4、SLC7A11和SLC1A5。GSEA和REACTOME分析确定了暴露于氨基酸剥夺的细胞中信号传导的改变，例如与应激反应、细胞周期和凋亡相关的途径。此外，主成分分析显示，与正常肿瘤组织相比，这六个AAT基因被很好地分为两个不同的簇。最后，对数秩分析证实，这组由六个AAT基因组成的基因的上调与结直肠癌、食道癌、肾癌和肺癌患者的不良预后相关。结论：六种AAT的上调在几种人类癌症中很常见，可能为评估结直肠癌、食道癌、肾癌和肺癌患者的预后提供有价值的诊断工具。

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来源期刊

Cell Journal CELL BIOLOGY-

CiteScore

3.40

自引率

5.00%

发文量

审稿时长

12 months

期刊介绍： The “Cell Journal (Yakhteh)“, formerly published as “Yakhteh Medical Journal”, is a quarterly English publication of Royan Institute. This journal focuses on topics relevant to cellular and molecular scientific areas, besides other related fields. The Cell J has been certified by Ministry of Culture and Islamic Guidance in 1999 and was accredited as a scientific and research journal by HBI (Health and Biomedical Information) Journal Accreditation Commission in 2000 which is an open access journal.