Journal of Infection in Developing Countries最新文献_第10页

Japanese spotted fever complicated with pleural effusion in Zhejiang province, China: a case report and literature review. 中国浙江省并发胸腔积液的日本斑疹热：病例报告和文献综述。

IF 1.4 4区医学

Journal of Infection in Developing Countries Pub Date : 2024-07-29 DOI: 10.3855/jidc.18354

Keting He, Shangci Chen, Liang Yu, Dongfan Wei, Xiaowei Xu

{"title":"Japanese spotted fever complicated with pleural effusion in Zhejiang province, China: a case report and literature review.","authors":"Keting He, Shangci Chen, Liang Yu, Dongfan Wei, Xiaowei Xu","doi":"10.3855/jidc.18354","DOIUrl":"https://doi.org/10.3855/jidc.18354","url":null,"abstract":"Introduction: Japanese spotted fever (JSF) mainly occurs in Japan; however, it has been increasingly reported in China. JSF is typically characterized by fever, rash, and eschar, in addition to non-specific symptoms. Yet, reports on the pulmonary indicators in JSF are limited. Herein, we report an unusual case of JSF associated with pleural effusion and pneumonia, in which the pathogen was identified via blood next-generation sequencing (NGS).Case presentation: We report a case of a 33-year-old woman who presented with fever for five days, rash for two days, and myalgia, fatigue, and edema for one day. She had recently been on vacation when an unknown insect bit her. The doctors at the local primary hospital considered a bacterial infection and administered dexamethasone, ceftriaxone, indomethacin, and anti-allergy agents, but the symptoms persisted. A rash without pruritus or pain developed gradually over the entire body and face. We considered rickettsial infection and administered doxycycline and levofloxacin. Metagenomic NGS from blood confirmed the presence of Rickettsia japonica (R. japonica). Abdominal computed tomography revealed bilateral pleural effusion with two atelectasis; patchy shadows with blurred edges, and uniform enhancement in both lower lungs. After several days of treatment, the symptoms and laboratory results improved. A literature review of the epidemiology of R. japonica and JSF in China, characteristics of JSF, and related pulmonary changes, and technology to diagnose JSF is provided.Conclusions: JSF has a variety of symptoms and is becoming increasingly popular in China. Clinical doctors need to identify it carefully.","PeriodicalId":49160,"journal":{"name":"Journal of Infection in Developing Countries","volume":"18 7","pages":"1135-1140"},"PeriodicalIF":1.4,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141856866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical features and risk factors for mortality in patients with Klebsiella pneumoniae bloodstream infections. 肺炎克雷伯氏菌血流感染患者的临床特征和死亡风险因素。

IF 1.4 4区医学

Journal of Infection in Developing Countries Pub Date : 2024-06-30 DOI: 10.3855/jidc.18649

Panpan Xu, Xijiang Zhang, Qingqing Chen, Qin Si, Xinhua Luo, Chuming Zhang, Zongguang He, Ronghai Lin, Cheng Zheng

{"title":"Clinical features and risk factors for mortality in patients with Klebsiella pneumoniae bloodstream infections.","authors":"Panpan Xu, Xijiang Zhang, Qingqing Chen, Qin Si, Xinhua Luo, Chuming Zhang, Zongguang He, Ronghai Lin, Cheng Zheng","doi":"10.3855/jidc.18649","DOIUrl":"10.3855/jidc.18649","url":null,"abstract":"Introduction: Concern about Klebsiella pneumoniae (K. pneumoniae) bloodstream infections (KP-BSIs) is widespread because of their high incidence and lethality. The aim of this study was to investigate the clinical features of, and risk factors for mortality caused by KP-BSIs.Methodology: This was a single-center retrospective observational study performed between 1 January 2019 and 31 December 2021, at a tertiary hospital. All patients with KP-BSIs were enrolled and their clinical data were retrieved from electronic medical records.Results: A total of 145 patients were included (121 in the survival group and 24 in the non-survival group). There was a higher proportion of lower respiratory tract infections in the non-survival group than in the survival group (33.3% vs. 12.4%) (p < 0.05). There was a higher proportion of multi drug resistant (MDR) strains of K. pneumoniae in the non-survival group than in the survival group (41.7% vs. 16.5%) (p < 0.05). Multivariate analysis revealed that sequential organ failure assessment (SOFA) score > 6.5 (OR, 13.71; 95% CI, 1.05-179.84), admission to the intensive care unit (ICU) (OR, 2.27; 95% CI, 0.26-19.61) and gastrointestinal bleeding (OR, 19.97; 95% CI, 1.11-361.02) were independent risk factors for death in patients with KP-BSIs.Conclusions: Among all KP-BSIs, a high proportion of K. pneumoniae originated from lower respiratory tract infections, and a high proportion of K. pneumoniae were MDR; however, mortality was not influenced. SOFA score > 6.5, admission to the ICU, and gastrointestinal bleeding were independent risk factors for death in patients with KP-BSI.","PeriodicalId":49160,"journal":{"name":"Journal of Infection in Developing Countries","volume":"18 6","pages":"843-850"},"PeriodicalIF":1.4,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141591834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fever of unknown origin: A 12-year case series in Colombia. 原因不明的发烧：哥伦比亚 12 年的系列病例。

IF 1.4 4区医学

Journal of Infection in Developing Countries Pub Date : 2024-06-30 DOI: 10.3855/jidc.18192

Juan Manuel Vásquez-Jiménez, Sebastián Mackenzie, Jorge Pulido-Arenas, Santiago Bernal-Macías, Javier Ricardo Garzon, Ivonne Tatiana Ordóñez, Néstor Fabián Correa, Oscar Muñoz

{"title":"Fever of unknown origin: A 12-year case series in Colombia.","authors":"Juan Manuel Vásquez-Jiménez, Sebastián Mackenzie, Jorge Pulido-Arenas, Santiago Bernal-Macías, Javier Ricardo Garzon, Ivonne Tatiana Ordóñez, Néstor Fabián Correa, Oscar Muñoz","doi":"10.3855/jidc.18192","DOIUrl":"https://doi.org/10.3855/jidc.18192","url":null,"abstract":"Background: Fever of unknown origin (FUO) is a diagnostic challenge with highly heterogeneous causes. Its etiology can change according to the studied regions, and the chance of reaching a diagnosis depends on available resources. The aim of this study is to describe the clinical characteristics, etiology and the usefulness of diagnostic aids in cases of FUO managed over 12 years in a Colombian reference center.Methodology: Single-institution retrospective case series. All cases of FUO between 2006 and 2017 were identified with the help of an electronic medical record search software. Cases of adults with fever for more than three weeks who remained undiagnosed after three days of hospitalization are described.Results: Of 1,009 cases evaluated, 112 cases met the inclusion criteria (median age 43 years, 66% men). The etiologies identified were infectious (31.2%), inflammatory (20.5%), neoplastic (14.3%), and miscellaneous (2.7%) diseases. 31.2% remained without etiological diagnosis. The most frequent conditions were tuberculosis (17%), Hodgkin's lymphoma (7.1%), systemic lupus erythematosus (6.3%), disseminated histoplasmosis, and adult Still's disease. Contrast tomography and biopsies were the studies that most frequently supported or confirmed the final diagnosis.Conclusions: This series of contemporary Latin American cases suggests that the categories of FUO etiologies are similar to those reported in studies from developed countries, with tuberculosis being the most frequent cause in our setting. Our results highlight the importance of tomography-guided invasive studies in the diagnostic approach to FUO.","PeriodicalId":49160,"journal":{"name":"Journal of Infection in Developing Countries","volume":"18 6","pages":"880-886"},"PeriodicalIF":1.4,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141591840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gastric microbiome composition in obese patients and normal weight subjects with functional dyspepsia. 患有功能性消化不良的肥胖患者和体重正常者的胃微生物组组成。

IF 1.4 4区医学

Journal of Infection in Developing Countries Pub Date : 2024-06-30 DOI: 10.3855/jidc.19304

Umut Gazi, Gunnur Kocer, Emrah Ruh, Can Holyavkin, Ozgur Tosun, Mustafa Celik, Aysegul Cort Donmez, Onur Birsen

{"title":"Gastric microbiome composition in obese patients and normal weight subjects with functional dyspepsia.","authors":"Umut Gazi, Gunnur Kocer, Emrah Ruh, Can Holyavkin, Ozgur Tosun, Mustafa Celik, Aysegul Cort Donmez, Onur Birsen","doi":"10.3855/jidc.19304","DOIUrl":"https://doi.org/10.3855/jidc.19304","url":null,"abstract":"Introduction: Despite the numerous studies demonstrating gut microbiota dysbiosis in obese subjects, there is no data on the association between obesity and gastric microbiota. The aim of this study was to address this gap in literature by comparing the composition of gastric microbiota in obese patients and a control group which included normal weight volunteers diagnosed with functional dyspepsia (FD).Methodology: A total of 19 obese patients, and 18 normal weight subjects with FD and normal endoscopy results were included in the study. The gastric tissue samples were collected from participants in both groups by bariatric surgery and endoscopy, respectively, and profiled using 16S ribosomal RNA gene sequencing.Results: There was no significant difference in the α-diversity scores, while distinct gastric microbial compositions were detected in both groups. Significantly lower levels of Bacteroidetes and Fusobacteria, and higher Firmicutes/Bacteroidetes ratio were recorded in the obese patients. A total of 15 bacterial genera exhibited significant difference in gastric abundance with Prevotella_7, Veillonella, Cupriavidus, and Acinetobacter, present in frequencies higher than 3% in at least one subject group.Conclusions: Our study suggests a significant association between obesity and gastric microbiome composition. Future studies with larger sample size and gastric samples from subjects without any gastrointestinal complications are required to confirm our conclusions.","PeriodicalId":49160,"journal":{"name":"Journal of Infection in Developing Countries","volume":"18 6","pages":"909-918"},"PeriodicalIF":1.4,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141591841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ILC2s induce Treg but not Th2-type immunity through IL-33/ST2 pathway in pulmonary tuberculosis. ILC2通过IL-33/ST2途径在肺结核中诱导Treg而非Th2型免疫。

IF 1.4 4区医学

Journal of Infection in Developing Countries Pub Date : 2024-06-30 DOI: 10.3855/jidc.18881

Qifeng Li, Quan Wang, Zhenhua Xu

{"title":"ILC2s induce Treg but not Th2-type immunity through IL-33/ST2 pathway in pulmonary tuberculosis.","authors":"Qifeng Li, Quan Wang, Zhenhua Xu","doi":"10.3855/jidc.18881","DOIUrl":"10.3855/jidc.18881","url":null,"abstract":"Introduction: We investigated the function of type 2 innate lymphoid cells (ILC2s) and IL-33 in pulmonary tuberculosis (PTB).Methodology: Peripheral blood samples were collected from PTB patients and healthy controls. The cytometric bead array was used to detect plasma IL-33, TGF-β, IL-4, IL-5, IL-6, IL-10, IL-13, and soluble ST2 (sST2). ILC2s, Th2, and Treg cells were detected with flow cytometry. Quantitative real-time PCR was used to measure mRNA levels. ILC2s were co-cultured with peripheral blood mononuclear cells and then intervened with IL-33 or anti-ST2 antibody + IL-33 in vitro. IL-4, IL-6, IL-5, IL-10, IL-13, and TGF-β levels were measured by enzyme-linked immunosorbent assay.Results: Compared with healthy controls, the levels of IL-33, sST2, TGF-β, IL-10, and IL-6 in the plasma of PTB patients were significantly higher. No significant difference was found in the plasma IL-4, IL-5, and IL-13 levels. Patients with PTB had significantly increased ILC2s proportion and mRNA levels of RAR-related orphan receptor α and GATA binding protein 3. After 48 h of IL-33 stimulation in vitro, Treg cell proportion significantly increased and the IL-10 level was significantly elevated. Treatment with anti-ST2 abolished these effects. No significant difference was found in cytokines of IL-4, IL-6, IL-5, IL-13, and TGF-β, or Th2 cells before and after IL-33 treatment. ILC2s proportion in peripheral blood was increased and plasma IL-33 was upregulated in PTB patients.Conclusions: IL-33 may promote the growth of ILC2s and the production of Treg-related cell cytokines, but not Th2-related cell cytokines, to participate in immune response to PTB.","PeriodicalId":49160,"journal":{"name":"Journal of Infection in Developing Countries","volume":"18 6","pages":"887-894"},"PeriodicalIF":1.4,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141591766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Incidence of cutaneous Leishmaniasis in humans during the COVID-19 pandemic in Baluchistan Province, Pakistan. 巴基斯坦俾路支斯坦省 COVID-19 大流行期间人类皮肤利什曼病发病率。

IF 1.4 4区医学

Journal of Infection in Developing Countries Pub Date : 2024-06-30 DOI: 10.3855/jidc.17781

Syeda Atiya Bukhari, Lamia Shafqat, Khawar Bilal, Ashiq Hussain, Shaista Gul, Shumaila Naz, Naseer Ali Shah, Emina Dervišević, Lejla Dervišević, Zurifa Ajanović, Hajra Manahil, Muhammad Aamir Ghafoor Chaudhary, Ying Wang, Haroon Ahmed, Jianping Cao

{"title":"Incidence of cutaneous Leishmaniasis in humans during the COVID-19 pandemic in Baluchistan Province, Pakistan.","authors":"Syeda Atiya Bukhari, Lamia Shafqat, Khawar Bilal, Ashiq Hussain, Shaista Gul, Shumaila Naz, Naseer Ali Shah, Emina Dervišević, Lejla Dervišević, Zurifa Ajanović, Hajra Manahil, Muhammad Aamir Ghafoor Chaudhary, Ying Wang, Haroon Ahmed, Jianping Cao","doi":"10.3855/jidc.17781","DOIUrl":"https://doi.org/10.3855/jidc.17781","url":null,"abstract":"Introduction: Cutaneous leishmaniasis (CL) is one of the neglected tropical diseases that affects impoverished communities throughout the world. In Pakistan CL is an endemic disease.Aims and objectives: This study aimed to determine the incidence of CL infection in the Baluchistan province of Pakistan from January 2020 to March 2022 during the COVID-19 pandemic.Methodology: A total of 1047 clinically suspected cases of CL from Bolan Medical College Hospital, Quetta, were followed up in the study. The data regarding the epidemiological characterstics, pathological information, and treatment of patients was collected.Results: Out of 1047 probable cases of CL, 594 (56.73%) cases were found to be positive for CL. Females had the highest infection rate, with the majority of reported cases being in the 0-9-year age group. Most CL cases were reported in April in the year 2020, with a few cases reported in June. But in the year 2021, the highest number of cases were reported in December. The number of overall cases has gradually increased in the year 2022, most likely because of the reduction in COVID-19 pandemic restrictions. The p value for the positive as compared to suspected cases in the years 2020, 2021, and 2022 was calculated as 0.8925, 0.8763, and 0.8535 respectively.Conclusions: Further epidemiological studies and health education campaigns are recommended to increase public awareness. It is strongly advised that local, provincial, and national health authorities establish and maintain effective leishmaniasis surveillance systems to promptly identify disease outbreaks and implement timely control measures.","PeriodicalId":49160,"journal":{"name":"Journal of Infection in Developing Countries","volume":"18 6","pages":"862-869"},"PeriodicalIF":1.4,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141591767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coexistence of β-lactamase genes and biofilm forming potential among carbapenem-resistant Acinetobacter baumannii in Lahore, Pakistan. 巴基斯坦拉合尔耐碳青霉烯类鲍曼不动杆菌中β-内酰胺酶基因与生物膜形成潜能的共存。

IF 1.4 4区医学

Journal of Infection in Developing Countries Pub Date : 2024-06-30 DOI: 10.3855/jidc.19119

Maqsood Arif, Amina Asif, Kiran Nazeer, Sikander Sultan, Saba Riaz

{"title":"Coexistence of β-lactamase genes and biofilm forming potential among carbapenem-resistant Acinetobacter baumannii in Lahore, Pakistan.","authors":"Maqsood Arif, Amina Asif, Kiran Nazeer, Sikander Sultan, Saba Riaz","doi":"10.3855/jidc.19119","DOIUrl":"https://doi.org/10.3855/jidc.19119","url":null,"abstract":"Introduction: Our goal was to investigate the antimicrobial resistance due to beta-lactamase genes and virulent determinants (biofilm-forming ability) expressed by Acinetobacter collected from health settings in Pakistan. A cross-sectional study was conducted for the molecular characterization of carbapenemases and biofilm-producing strains of Acinetobacter spp.Methodology: Two twenty-three imipenem-resistant Acinetobacter isolates were analyzed from 2020 to 2023.The combination disk test and modified hodge test were performed. Biofilm forming ability was determined by polystyrene tube assay. Multiplex polymerase chain reaction (PCR) for virulent and biofilm-forming genes, and 16S rRNA sequencing were performed.Results: 118 (52.9%) carbapenem-resistant Acinetobacter (CR-AB) were isolated from wounds and pus, 121 (54.2%) from males, and 92 (41.2%) from 26-50-years-olds. More than 80% of strains produced β-lactamases and carbapenemases. Based on the PCR amplification of the ITS gene, 174 (78.0%) CR-AB strains were identified from CR-Acinetobacter non-baumannii (ANB). Most CR-AB were strong and moderate biofilm producers. Genetic analysis revealed the blaOXA-23, blaTEM, blaCTX-M blaNDM-1 and blaVIM were prevalent in CR-AB with frequencies 91 (94.8%), 68 (70.8%), 19 (19.7%), 53 (55.2%), 2 (2.0%) respectively. Among virulence genes, OmpA was dominant in CR-AB isolates from wound (83, 86.4%), csuE 63 (80.7%) from non-wound specimens and significantly correlated with blaNDM and blaOXA genes. Phylogenetic analysis revealed three different clades for strains based on specimens.Conclusions: CR-AB was highly prevalent in Pakistan and associated with wound infections. The genes, blaOXA-23, blaTEM, blaCTX-M, and blaNDM-1 were detected in CR-AB. Most CR-AB were strong biofilm producers with virulent genes OmpA and csuE.","PeriodicalId":49160,"journal":{"name":"Journal of Infection in Developing Countries","volume":"18 6","pages":"943-949"},"PeriodicalIF":1.4,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141591835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and associated factors of bacterial vaginosis among pregnant women in Hue, Vietnam. 越南顺化市孕妇细菌性阴道病的发病率和相关因素。

IF 1.4 4区医学

Journal of Infection in Developing Countries Pub Date : 2024-06-30 DOI: 10.3855/jidc.18949

Anh Tc Nguyen, Na Tl Nguyen, Trang Tq Tran, Tuyen T Nguyen, Thu Ta Hoang, Linh M Tran, Khoi Tuan Tran, Thanh Dc Phan, Xuan T Nguyen, Tam M Le, An V Le, Andres Salumets, Reet Mändar

{"title":"Prevalence and associated factors of bacterial vaginosis among pregnant women in Hue, Vietnam.","authors":"Anh Tc Nguyen, Na Tl Nguyen, Trang Tq Tran, Tuyen T Nguyen, Thu Ta Hoang, Linh M Tran, Khoi Tuan Tran, Thanh Dc Phan, Xuan T Nguyen, Tam M Le, An V Le, Andres Salumets, Reet Mändar","doi":"10.3855/jidc.18949","DOIUrl":"https://doi.org/10.3855/jidc.18949","url":null,"abstract":"Introduction: Bacterial vaginosis (BV) is the most frequent vaginal infection affecting women of childbearing age worldwide. It is associated with significant adverse healthcare outcomes, especially during pregnancy. Although screening for BV could reduce potential pregnancy-related obstetric complications, there is no routine screening of pregnant women for BV in Vietnam. We aimed to identify the prevalence of BV among pregnant women and the associated factors in two tertiary hospitals in Hue, Vietnam.Methodology: This cross-sectional descriptive study included 885 pregnant women in third trimester, who received routine antenatal care in the Hue Central Hospital and Hue University Hospital of Medicine and Pharmacy, Hue city, Thua Thien Hue province, Vietnam. Gram-stained vaginal smears were used for calculating the Nugent score and recording the fungal elements.Results: In total, 435 (49.1%) women had a normal BV score, 352 (39.8%) had intermediate vaginal microbiota, and 98 (11.1%) had BV. Among the 98 women with BV, 71 (72.4%) also had fungal infection. There was a significant association of BV with discharge (p = 0.004) and abnormal cervix (p = 0.014). BV was significantly more frequent among the women who reported previous abortion or miscarriage (p = 0.007).Conclusions: About a tenth of women in Thua Thien Hue province have BV in the third trimester of pregnancy being associated with previous adverse outcome. Discharge with fishy odour is still a characteristic feature among subtle clinical presentations of BV. Better awareness about this disease and routine test-and-treat management during pregnancy may improve pregnancy outcome.","PeriodicalId":49160,"journal":{"name":"Journal of Infection in Developing Countries","volume":"18 6","pages":"925-931"},"PeriodicalIF":1.4,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141591862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic epidemiology of SARS-CoV-2 in Senegal in 2020-2021. 2020-2021 年塞内加尔 SARS-CoV-2 基因组流行病学。

IF 1.4 4区医学

Journal of Infection in Developing Countries Pub Date : 2024-06-30 DOI: 10.3855/jidc.17796

Adjiratou Aissatou Ba, Romain Coppée, Assane Dieng, Jarra Manneh, Mengue Fall, Khadim Gueye, Pauline Yacine Sene, Samba Ndiour, Dianké Samaté, Pascaline Manga, Oumy Diop Diongue, Abdoulaye Camara, Abdou Padane, Sada Diallo, Sokhna Bousso Gueye, Makhtar Camara, Abdul Karim Sesay, Coumba Toure-Kane, Souleymane Mboup, Charlotte Charpentier, Halimatou Diop-Ndiaye

{"title":"Genomic epidemiology of SARS-CoV-2 in Senegal in 2020-2021.","authors":"Adjiratou Aissatou Ba, Romain Coppée, Assane Dieng, Jarra Manneh, Mengue Fall, Khadim Gueye, Pauline Yacine Sene, Samba Ndiour, Dianké Samaté, Pascaline Manga, Oumy Diop Diongue, Abdoulaye Camara, Abdou Padane, Sada Diallo, Sokhna Bousso Gueye, Makhtar Camara, Abdul Karim Sesay, Coumba Toure-Kane, Souleymane Mboup, Charlotte Charpentier, Halimatou Diop-Ndiaye","doi":"10.3855/jidc.17796","DOIUrl":"https://doi.org/10.3855/jidc.17796","url":null,"abstract":"Introduction: In Senegal, molecular diagnosis was widely used for the detection and management of COVID-19 patients. However, genomic surveillance was very limited in the public sector. This study aimed to share the experience of a Senegalese public sector laboratory in response to the COVID-19 pandemic, and to describe the distribution of variants circulating in 2020 and 2021.Methodology: From July 2020 to December 2021, SARS-CoV-2 qRT-PCR was performed on nasopharyngeal samples from travelers and symptomatic patients at the Bacteriology and Virology Laboratory (LBV) of the Aristide le Dantec University Teaching Hospital. Samples with a cycle threshold (Ct) ≤ 30 were selected for whole-genome sequencing (WGS) using the Nanopore technology. In-house scripts were developed to study the spatial and temporal distribution of SARS-CoV-2 variants in Senegal, using our sequences and those retrieved from the GISAID database.Results: Of 8,207 patients or travelers screened for SARS-CoV-2, 970 (11.8%) were positive and 386 had a Ct ≤ 30. WGS was performed on 133 samples. Concomitantly with high-quality sequences deposited in the GISAID database covering nine cities in Senegal in 2020 and 2021 (n = 1,539), we observed a high circulation of the 20A (B.1, B.1.416 and B.1.620) and 20B (B.1.1.420) lineages in 2020, while most of the samples belonged to Delta variants (AY34 and AY.34.1, 22%) in 2021.Conclusions: Despite its late involvement, COVID-19 diagnosis was routinely performed in LBV, but genomic characterization remained challenging. The genomic diversity of SARS-CoV-2 strains in Senegal reflected that observed worldwide during the first waves of the pandemic.","PeriodicalId":49160,"journal":{"name":"Journal of Infection in Developing Countries","volume":"18 6","pages":"851-861"},"PeriodicalIF":1.4,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141591842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Vibrio parahaemolyticus isolated from clinical specimens and oysters in Thailand. 从泰国临床样本和牡蛎中分离出的副溶血性弧菌的特征。

IF 1.4 4区医学

Journal of Infection in Developing Countries Pub Date : 2024-06-30 DOI: 10.3855/jidc.18470

Nipaporn Tewawong, Siriporn Kowaboot, Sudaporn Kengkarn, Unchana Thawornwan, Pongrama Ramasoota, Orasa Suthienkul

{"title":"Characterization of Vibrio parahaemolyticus isolated from clinical specimens and oysters in Thailand.","authors":"Nipaporn Tewawong, Siriporn Kowaboot, Sudaporn Kengkarn, Unchana Thawornwan, Pongrama Ramasoota, Orasa Suthienkul","doi":"10.3855/jidc.18470","DOIUrl":"https://doi.org/10.3855/jidc.18470","url":null,"abstract":"Introduction: Vibrio parahaemolyticus is a common pathogen that can cause seafood-borne gastroenteritis in humans. We determined the prevalence and characteristics of V. parahaemolyticus isolated from clinical specimens and oysters in Thailand.Methodology: Isolates of V. parahaemolyticus from clinical specimens (n = 77) and oysters (n = 224) were identified by biochemical testing, polymerase chain reaction (PCR) assays, and serotyping. The toxin genes, antimicrobial resistance, and β-lactamase production were determined.Results: A total of 301 isolates were confirmed as V. parahaemolyticus by PCR using specific primers for the toxR gene. The majority of clinical isolates carried the tdh+/trh- genotype (82.1%), and one of each isolate was tdh-/trh+ and tdh+/trh+ genotypes. One isolate from oyster contained the tdh gene and another had the trh gene. Twenty-six serotypes were characterized among these isolates, and O3:K6 was the most common (37.7%), followed by OUT:KUT, and O4:K9. In 2010, most clinical and oyster isolates were susceptible to antibiotics, with the exception of ampicillin. In 2012, clinical isolates were not susceptible to cephalothin (52.4%), streptomycin (95.2%), amikacin (66.6%), kanamycin (61.9%), and erythromycin (95.2%), significantly more frequently than in 2010. More than 95% of isolates that were not susceptible to ampicillin produced β-lactamase enzymes.Conclusions: We found toxin genes in two oyster isolates, and the clinical isolates that were initially determined to be resistant to several antibiotics. Toxin genes and antimicrobial susceptibility profiles of V. parahaemolyticus from seafood and environment should be continually monitored to determine the spread of toxin and antimicrobial resistance genes.","PeriodicalId":49160,"journal":{"name":"Journal of Infection in Developing Countries","volume":"18 6","pages":"900-908"},"PeriodicalIF":1.4,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141591833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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