Human Vaccines & Immunotherapeutics最新文献_第10页

Number of medical facilities within driving distance of residence and influenza vaccination status in Japan: A cross-sectional study. 日本居民驾车距离内的医疗设施数量与流感疫苗接种状况：一项横断面研究

IF 4.1 4区医学

Human Vaccines & Immunotherapeutics Pub Date : 2025-12-01 Epub Date: 2024-12-20 DOI: 10.1080/21645515.2024.2441407

Masaki Machida, Shigeru Inoue, Tomoki Nakaya, Shinji Fukushima, Wakaba Fukushima, Megumi Hara, Takahiro Tabuchi

{"title":"Number of medical facilities within driving distance of residence and influenza vaccination status in Japan: A cross-sectional study.","authors":"Masaki Machida, Shigeru Inoue, Tomoki Nakaya, Shinji Fukushima, Wakaba Fukushima, Megumi Hara, Takahiro Tabuchi","doi":"10.1080/21645515.2024.2441407","DOIUrl":"https://doi.org/10.1080/21645515.2024.2441407","url":null,"abstract":"Vaccine hesitancy primarily consists of confidence, complacency, and convenience, including geographical accessibility. However, few studies in developed countries have focused on the association between geographical accessibility to vaccination services and vaccination status and previous studies have shown conflicting results. This cross-sectional study aimed to clarify the association between the number of medical facilities within a 15-minute driving distance from the place of residence and influenza vaccination status, using a large nationwide internet survey in Japan (n = 27,440). Postal codes were used to determine participants' place of residence and the number of medical facilities within a 15-minute drive was calculated using geographic information system data. Participants were classified into quartiles based on the number of medical facilities nearby. They reported their influenza vaccination status over the past year. Modified Poisson regression analysis was conducted with influenza vaccination status as the dependent variable and the number of medical facilities nearby as the independent variable, adjusted for covariates stratified by age (under 65 years: or 65 years and older). Among participants aged 65 years and older, those with fewer medical facilities within a 15-minute drive of their place of residence were significantly less likely to have received an influenza vaccination than those with more medical facilities nearby (prevalence ratio in Q1 relative to Q4: 0.93, 95% confidence interval 0.87-0.99). In contrast, no association was observed among participants aged under 65 years. Geographical accessibility to vaccination services may influence vaccine hesitancy among older adults in Japan, a developed country.","PeriodicalId":49067,"journal":{"name":"Human Vaccines & Immunotherapeutics","volume":"21 1","pages":"2441407"},"PeriodicalIF":4.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strategies for introducing and implementing vaccines for adults into national immunization programs in Europe: Good practices and key insights of the adult immunization board meeting. 在欧洲国家免疫规划中引入和实施成人疫苗的战略：成人免疫委员会会议的良好做法和关键见解。

IF 4.1 4区医学

Human Vaccines & Immunotherapeutics Pub Date : 2025-12-01 Epub Date: 2025-01-17 DOI: 10.1080/21645515.2025.2451487

Sara Boccalini, Angela Bechini, Marco Del Riccio, Birgit Weinberger, Jacek Wysocki, Domenico Martinelli, Ole Wichmann, Tiina Likki, Greet Hendrickx, Pierre Van Damme, Chloé Wyndham-Thomas, Paolo Bonanni, Jade Pattyn

{"title":"Strategies for introducing and implementing vaccines for adults into national immunization programs in Europe: Good practices and key insights of the adult immunization board meeting.","authors":"Sara Boccalini, Angela Bechini, Marco Del Riccio, Birgit Weinberger, Jacek Wysocki, Domenico Martinelli, Ole Wichmann, Tiina Likki, Greet Hendrickx, Pierre Van Damme, Chloé Wyndham-Thomas, Paolo Bonanni, Jade Pattyn","doi":"10.1080/21645515.2025.2451487","DOIUrl":"10.1080/21645515.2025.2451487","url":null,"abstract":"In April 2024, the Adult Immunization Board convened a technical meeting to explore the latest strategies and identify exemplary approaches regarding the implementation of vaccines for adults into Europe's National Immunization Programs (NIPs). The meeting was built around three pillars: decision making for introducing a new vaccine, implementation, monitoring, and evaluation. The increasing number of new vaccines available in a context of competing health priorities warrants transparent and evidence-based decision-making processes for vaccine introduction. In Europe, burden of disease, vaccine efficacy or effectiveness, and safety are universally used decision-making criteria. While economic evaluations and the quality of evidence are being increasingly considered, public acceptance, equity, and operational criteria remain underutilized. Vaccine implementation requires planning and coordination. Implementation activities discussed during the meeting were vaccine program goals, target population identification, communication, training of healthcare professionals, and involvement of pharmacists. Once operational, NIPs are to be monitored in terms of safety, effectiveness, and impact. Implementation science as well as behavioral and cultural insights can be used to identify tangible interventions to improve uptake. Meeting discussions allowed to identify adult vaccine implementation barriers and possible strategies to overcome them. Enhanced cooperation and coordination throughout the Europe region were recognized as critical components of progress.","PeriodicalId":49067,"journal":{"name":"Human Vaccines & Immunotherapeutics","volume":"21 1","pages":"2451487"},"PeriodicalIF":4.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143014648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mini-review on the therapeutic vaccines targeting chronic infectious diseases: Evaluation system of therapeutic vaccines targeting HPV and EBV-related cancers.

IF 4.1 4区医学

Human Vaccines & Immunotherapeutics Pub Date : 2025-12-01 Epub Date: 2025-02-16 DOI: 10.1080/21645515.2025.2457187

Seungkyun Woo, Pil-Gu Park, Timothy An, Munazza Fatima, Ye-Eun Moon, Seok-Yong Lee, Hyewon Youn, Kee-Jong Hong

{"title":"Mini-review on the therapeutic vaccines targeting chronic infectious diseases: Evaluation system of therapeutic vaccines targeting HPV and EBV-related cancers.","authors":"Seungkyun Woo, Pil-Gu Park, Timothy An, Munazza Fatima, Ye-Eun Moon, Seok-Yong Lee, Hyewon Youn, Kee-Jong Hong","doi":"10.1080/21645515.2025.2457187","DOIUrl":"10.1080/21645515.2025.2457187","url":null,"abstract":"Chronic infectious diseases are threatening human health today, and their public health severity is increasing. The efficacy issues of drugs and the increase in drug-resistant pathogens require new response strategies for chronic infectious diseases, and therapeutic vaccines have recently been proposed as an effective alternative. However, research on therapeutic vaccines is still relatively underdeveloped. To solve this problem, an accurate understanding of the status and the challenge at hand of therapeutic vaccines targeting chronic infectious diseases is needed. In the present review, we provide an overview of the latest research trends in therapeutic vaccines targeting chronic infectious diseases and summarize the development status of therapeutic vaccines currently undergoing clinical research, focusing on the cases of human papillomavirus (HPV) and Epstein-Barr virus (EBV) as representative examples. We highlight the importance of standard methods for the evaluation of therapeutic vaccine, focusing on the cell-mediated immune response, which might accelerate therapeutic vaccine development.","PeriodicalId":49067,"journal":{"name":"Human Vaccines & Immunotherapeutics","volume":"21 1","pages":"2457187"},"PeriodicalIF":4.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunotherapy-induced microsatellite instability status shift in recurrent perihilar cholangiocarcinoma: A case report. 复发性肝周胆管癌中免疫疗法诱发的微卫星不稳定状态转变：病例报告。

IF 4.1 4区医学

Human Vaccines & Immunotherapeutics Pub Date : 2025-12-01 Epub Date: 2025-02-25 DOI: 10.1080/21645515.2025.2471226

Hailing Yu, Tan Deng, Hongbing Liu

{"title":"Immunotherapy-induced microsatellite instability status shift in recurrent perihilar cholangiocarcinoma: A case report.","authors":"Hailing Yu, Tan Deng, Hongbing Liu","doi":"10.1080/21645515.2025.2471226","DOIUrl":"10.1080/21645515.2025.2471226","url":null,"abstract":"Immunotherapy revolutionized the treatment of biliary tract tumors and tumors with high microsatellite instability (MSI-H). This paper reports a 52-year-old woman with recurrent perihilar cholangiocarcinoma. The tumor was initially microsatellite stable (MSS) and proficient mismatch repair (pMMR) but shifted to MSI-H and deficient mismatch repair (dMMR) after combined immunotherapy. Following laparoscopic radical resection for jaundice, stage IV recurrence was diagnosed. Genetic testing revealed the MSS status. Subsequent treatment with camrelizumab and lenvatinib led to a partial response. Ovarian metastases, removed due to abdominal symptoms, exhibited dMMR and MSI-H. The mismatch in MSI status between the primary tumor and metastases suggests tumor heterogeneity and the influence of spatial or temporal factors. This shift can have important clinical significance since MSI-H is associated with significant responses to immune checkpoint inhibitors. MSI-H should be systematically tested in tumors and metastases to personalize treatments. MSI heterogeneity is not only rare but potentially has implications for treatment personalization and prognosis in patients with cholangiocarcinoma. This case highlights the dynamic changes in tumor characteristics during immunotherapy.","PeriodicalId":49067,"journal":{"name":"Human Vaccines & Immunotherapeutics","volume":"21 1","pages":"2471226"},"PeriodicalIF":4.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11864312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Respiratory syncytial virus immunization with nirsevimab: Acceptance and satisfaction assessment in infants and risk groups in the region of Murcia (Spain).

IF 4.1 4区医学

Human Vaccines & Immunotherapeutics Pub Date : 2025-12-01 Epub Date: 2025-03-03 DOI: 10.1080/21645515.2025.2471700

Jaime Jesús Pérez Martín, María de la Cruz Gómez Moreno, Susana Sánchez Manresa, María Del Pilar Ros Abellán, Matilde Zornoza-Moreno

{"title":"Respiratory syncytial virus immunization with nirsevimab: Acceptance and satisfaction assessment in infants and risk groups in the region of Murcia (Spain).","authors":"Jaime Jesús Pérez Martín, María de la Cruz Gómez Moreno, Susana Sánchez Manresa, María Del Pilar Ros Abellán, Matilde Zornoza-Moreno","doi":"10.1080/21645515.2025.2471700","DOIUrl":"10.1080/21645515.2025.2471700","url":null,"abstract":"Respiratory syncytial virus (RSV) is a leading cause of respiratory infections in infants under two years of age, often resulting in bronchiolitis and pneumonia, which contribute to high hospitalization rates. The recent approval of nirsevimab, a long-acting monoclonal antibody, has provided an immunization alternative for infants, addressing the substantial burden of RSV. This study aimed to evaluate acceptance and satisfaction among parents or legal guardians of infants who were candidates for immunization with nirsevimab in the Region of Murcia (Spain) during the 2023-2024 campaign. A cross-sectional survey, encompassing 1692 parents of immunized infants and 219 of non-immunized infants, revealed a high acceptance rate for nirsevimab, with 87% of parents indicating willingness to immunize a future child and 86.6% willing to recommend it. Concerns about safety and side effects were the primary reasons for hesitation among parents who did not immunize their infants. These findings underscore the importance of enhancing educational efforts in future campaigns to address safety concerns, thereby supporting broader RSV immunization coverage in the pediatric population.","PeriodicalId":49067,"journal":{"name":"Human Vaccines & Immunotherapeutics","volume":"21 1","pages":"2471700"},"PeriodicalIF":4.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11881857/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143544062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of COVID-19 pandemic on influenza vaccination rates among healthcare workers and the general population in Saudi Arabia: A meta-analysis.

IF 4.1 4区医学

Human Vaccines & Immunotherapeutics Pub Date : 2025-12-01 Epub Date: 2025-03-11 DOI: 10.1080/21645515.2025.2477954

Salah Alshagrawi, Ahmed Hazazi

引用次数: 0

Factors influencing vaccine hesitancy among United Kingdom adolescents in a senior high school environment and actions to address it.

IF 4.1 4区医学

Human Vaccines & Immunotherapeutics Pub Date : 2025-12-01 Epub Date: 2025-03-11 DOI: 10.1080/21645515.2025.2475599

Francis Drobniewski, Marcia Ashmi, Raheelah Ahmad, Changchunzi He, Marianna Bogdanova, Alan Garbacz, Ahmed Moustafa

{"title":"Factors influencing vaccine hesitancy among United Kingdom adolescents in a senior high school environment and actions to address it.","authors":"Francis Drobniewski, Marcia Ashmi, Raheelah Ahmad, Changchunzi He, Marianna Bogdanova, Alan Garbacz, Ahmed Moustafa","doi":"10.1080/21645515.2025.2475599","DOIUrl":"10.1080/21645515.2025.2475599","url":null,"abstract":"We explored adolescent viewpoints on vaccines and hesitancy using an anonymized, validated, self-completed electronic questionnaire amongst state-school Year 12-13 adolescents in London, UK. As the response rate was low (Cohort 1; n = 112/486, 23.0%), we repeated the survey with incoming students (cohort 2, n = 256/275; 93%). A focus group (n = 31) evaluated international HPV campaign posters. Cohort 1 participants were 82.1% female, 13.4% male, and ethnically diverse (32.1% Asian/Asian British, 29.5% Black/Black British, 25.9% White, 7.1% Mixed); Cohort 2 were 80% female, 18% male, with participants identifying as 38% Black/Black British, 34% Asian/Asian British, 15% White, 12% Mixed/Others. Across both cohorts, participants believed childhood vaccinations were safe (Cohort 1 = 95.8%, Cohort 2 = 91%). COVID-19 vaccination uptake was higher in Cohort 1 than 2 (76.8% vs 67%), with fewer participants believing it was adequately tested (56.3% vs 47%). Support for mandatory COVID-19 vaccination for healthcare workers (HCW) was high (Cohort 1:77.8-79% \"all\" vs \"patient-facing HCWs;\" Cohort 2 = 62-64%). Similar patterns were observed for mandatory influenza vaccination (Cohort 1: 62.5-66.7%; Cohort 2: 62-63%). Vaccination decisions in Cohort 2 were primarily influenced by parents (96%), healthcare providers (48%), and school friends (36%) (Cohort 1 = 30.2%, 19.6%, and 12%, respectively). Recommendations by doctors, experts and parents (but not politicians) and school-based vaccine availability boosted vaccine confidence. Social media had minimal impact. Most participants received HPV vaccine (Cohort 1 = 83.1%, Cohort 2 = 77.2%). International HPV posters received strong but mixed support; direct design input from the target group is needed to ensure the success of visual vaccine promotions.","PeriodicalId":49067,"journal":{"name":"Human Vaccines & Immunotherapeutics","volume":"21 1","pages":"2475599"},"PeriodicalIF":4.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11901369/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143598014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expanding vaccination provider types and administration sites can increase vaccination uptake: A systematic literature review of the evidence in non-United States geographies. 扩大疫苗接种提供者类型和接种地点可提高疫苗接种率：对非美国地区证据的系统文献综述。

IF 4.1 4区医学

Human Vaccines & Immunotherapeutics Pub Date : 2025-12-01 Epub Date: 2025-03-02 DOI: 10.1080/21645515.2025.2463732

Anna Larson, Priya Shanmugam, Rachel Mitrovich, Divya Vohra, Aimee J Lansdale, Amanda L Eiden

{"title":"Expanding vaccination provider types and administration sites can increase vaccination uptake: A systematic literature review of the evidence in non-United States geographies.","authors":"Anna Larson, Priya Shanmugam, Rachel Mitrovich, Divya Vohra, Aimee J Lansdale, Amanda L Eiden","doi":"10.1080/21645515.2025.2463732","DOIUrl":"10.1080/21645515.2025.2463732","url":null,"abstract":"Vaccination is a successful public health intervention; however, vaccine-preventable diseases continue to pose global health risks due to insufficient uptake. Expanding authority for \"alternative\" or complementary healthcare providers to administer vaccinations, as well as approving additional non-clinical vaccination sites, could improve access to and uptake of vaccines. The value of complementary providers and expanded sites has been documented in the United States; however, there is limited evidence in geographies outside the United States. To address this gap, we conducted a systematic literature review to identify studies that evaluated vaccination by complementary providers and/or at expanded sites outside of the United States. Of 943 identified records, 18 met our inclusion criteria and were conducted in Australia (4), Canada (6), the United Kingdom (3), Peru (2), Cameroon (1), or in multiple geographies (2). All studies demonstrated that expanding provider types and sites could positively impact vaccine uptake and/or provide additional benefits.","PeriodicalId":49067,"journal":{"name":"Human Vaccines & Immunotherapeutics","volume":"21 1","pages":"2463732"},"PeriodicalIF":4.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11881856/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Administration of non-national immunization program vaccines for children under six in a rural county, Henan Province: Did costs matter? 河南省某农村县6岁以下儿童非国家免疫规划疫苗管理：费用重要吗？

IF 4.1 4区医学

Human Vaccines & Immunotherapeutics Pub Date : 2025-12-01 Epub Date: 2025-01-21 DOI: 10.1080/21645515.2025.2454744

Miaomiao Yin, Yuan Cao, Xiaolin Xu, Hanzhi Peng, Yu Wang, Qian Long

{"title":"Administration of non-national immunization program vaccines for children under six in a rural county, Henan Province: Did costs matter?","authors":"Miaomiao Yin, Yuan Cao, Xiaolin Xu, Hanzhi Peng, Yu Wang, Qian Long","doi":"10.1080/21645515.2025.2454744","DOIUrl":"https://doi.org/10.1080/21645515.2025.2454744","url":null,"abstract":"This study aimed to investigate caregivers' administration of non-National Immunization Program (NIP) vaccines in rural China, and examine health system, individual, and social determinants. A cross-sectional survey (n = 1051) was conducted from July to October in 2022 in a rural county of Henan Province. Caregivers of children under six who came to township health centers for child vaccination were interviewed. Cross-tabulation and multivariate logistic regressions were used to examine the administration rate and associated factors. Qualitative interviews were conducted with healthcare professionals (n = 4) and caregivers (Focus Group Discussions, n = 4) to understand local policies, routine practices, and caregivers' experience with the administration of non-NIP vaccines. A framework approach was used to analyze qualitative data. Quantitative and qualitative data were integrated during the interpretation of the results. The administration rate for non-NIP vaccines remained low in rural Henan, 26.7% of children had not received any non-NIP vaccines, 43.5% had received 1-2 types, and around 30% had received 3-5 types. There were no significant differences in the administration of non-NIP vaccines between left-behind and non-left-behind children after adjusting for characteristics of children, caregivers, and households. Qualitative findings reflected that the high cost of non-NIP vaccines was a primary factor influencing caregivers' vaccination decision. Poor communication between physicians and caregivers was another significant factor, which was caused by low retention of healthcare workers, a shortage of professionals, and insufficient financial incentives for physicians. This study enriches the evidence on non-NIP vaccination among rural children, particularly for the vulnerable group of left-behind children.","PeriodicalId":49067,"journal":{"name":"Human Vaccines & Immunotherapeutics","volume":"21 1","pages":"2454744"},"PeriodicalIF":4.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143014638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modifying non-coding regions of linear DNA vaccines to explore the interplay of expression and inflammation in immunogenicity. 修饰线性DNA疫苗的非编码区以探索免疫原性中表达与炎症的相互作用。

IF 4.1 4区医学

Human Vaccines & Immunotherapeutics Pub Date : 2025-12-01 Epub Date: 2025-01-20 DOI: 10.1080/21645515.2024.2430826

David C Stirling, Maria de Miguel Ferrer, Sungwon Kim, Madina Wane, Daniel Kysh, Lisa J Caproni, John S Tregoning

{"title":"Modifying non-coding regions of linear DNA vaccines to explore the interplay of expression and inflammation in immunogenicity.","authors":"David C Stirling, Maria de Miguel Ferrer, Sungwon Kim, Madina Wane, Daniel Kysh, Lisa J Caproni, John S Tregoning","doi":"10.1080/21645515.2024.2430826","DOIUrl":"https://doi.org/10.1080/21645515.2024.2430826","url":null,"abstract":"The COVID-19 pandemic highlighted the need for rapidly deployable, flexible vaccine platforms; particularly RNA which is now being explored for several other pathogens. DNA vaccines have potential advantages over RNA, including cost of manufacture, ease of storage and potentially lower reactogenicity. However, they have historically underperformed in large animals and human trials due to low immunogenicity. The interplay between antigen expression and the innate immune response impacts the overall immune response to DNA vaccines. Here, we explore the effect of altering non-coding 5' regions, on the immunogenicity of a closed linear DNA platform, Doggybone™ DNA (dbDNATM), produced by a rapid and scalable cell-free method. Using a mouse model, we found that enhancer sequences and DNA targeting sequences (DTS) increased influenza virus hemagglutinin (HA) expression and improved immune responses. Additional CpG motifs did not provide any immune benefit. We also found that the effect of non-coding sequences was target specific, with differing effects in influenza HA, SARS-CoV-2 Spike and eGFP constructs. To separate the effects of immune sensing of the DNA construct and the expression of the encoded antigen, we combined a separate CpG oligodeoxynucleotide (ODN) with the highest expressing DNA vaccine; we observed reduced expression, but higher inflammation resulting in equivalent immunogenicity. Further refinement is required to fully understand the interplay of factors required for the induction of protective immunity by DNA vaccines.","PeriodicalId":49067,"journal":{"name":"Human Vaccines & Immunotherapeutics","volume":"21 1","pages":"2430826"},"PeriodicalIF":4.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143014662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0