Oral Surgery Oral Medicine Oral Pathology Oral Radiology最新文献

{"title":"Oral plasma cell mucositis: a series of 3 cases with a focus on screening for systemic IgG4 disease","authors":"Sarah Fitzpatrick,&nbsp;Mohammed N. Islam,&nbsp;Indraneel Bhattacharyya,&nbsp;Saja Alramadhan","doi":"10.1016/j.oooo.2025.04.022","DOIUrl":"10.1016/j.oooo.2025.04.022","url":null,"abstract":"<div><div>Plasma cell mucositis (PCM) is an idiopathic generalized oral inflammatory condition with no consensus on optimal treatment strategy. Hypersensitivity may account for some cases, but etiology and causative factors mostly remain unknown. While more commonly associated with salivary gland involvement, systemic IgG4 disease may manifest as PCM. The reliability and calibration of IgG4 immunohistochemistry (IHC) or serum IgG4 testing for diagnosis of PCM remains uncertain. We present a series of three cases of oral PCM demonstrating the challenges in screening and management of this condition. The cases involved 71, 80, and 70-year-old males respectively. All cases initially affected the gingiva but spread to adjacent mucosa appearing as diffusely erythematous and edematous areas with superficial ulceration. Case 1 also reported a history of pancreatitis, and Case 3 reported sicca symptoms along with history of pancreatic and thyroid disease. Microscopically plasma cell mucositis was confirmed with all cases demonstrating polyclonality on kappa/lambda testing. Cases 1 and 3 also showed fibrinogen positivity on concurrent direct immunofluorescence testing, with Case 2 being negative. Cases 1 and 2 demonstrated more than 10 IgG4 cells per high power field on IHC testing, and Case 3 was unavailable for IHC testing. Case 1 was eventually diagnosed with systemic IgG4 disease after serum testing, and Cases 2 and 3 showed normal serum IgG4 testing results. None of the cases reported associated hypersensitivity to foods or oral care products. Cases 1 and 2 and were unresponsive to topical and systemic steroids, topical calcineurin inhibitors, and doxycycline. Case 3 was unresponsive to topical steroids and methotrexate. Case 1 eventually improved with rituximab, Case 2 remained stable, and Case 3 was treated partially successfully with mycophenolate. Full resolution was not achieved in any case. These cases demonstrate the need for additional consensus of best practice screening and treatment methods in PCM.</div></div>","PeriodicalId":49010,"journal":{"name":"Oral Surgery Oral Medicine Oral Pathology Oral Radiology","volume":"140 3","pages":"Page e74"},"PeriodicalIF":2.0,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144670787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and diagnosis of HPV-driven oral intraepithelial neoplasia by healthcare professionals: a case series","authors":"Jessica Snider ,&nbsp;Sara Ismile ,&nbsp;Mary Richardson","doi":"10.1016/j.oooo.2025.04.007","DOIUrl":"10.1016/j.oooo.2025.04.007","url":null,"abstract":"<div><h3>Introduction</h3><div>Human papillomavirus (HPV) is a well-known virus that can induce oncogenesis in multiple anatomic locations. In the head and neck, oropharyngeal carcinoma has become prevalent in recent decades. Notably, HPV-driven dysplasia can be seen in the oral cavity, an easily visualized site during physical exam. Understanding of common sites in the oral cavity and the patient populations affected by HPV-associated oral intraepithelial neoplasia can lead to early detection and diagnosis by dental practitioners and pathologists. The aim of this study is to describe cases of HPV-driven intraepithelial neoplasia in the context of patient population and presentation and to raise awareness of this lesser-known entity.</div></div><div><h3>Methods</h3><div>Four cases diagnosed as oral intraepithelial neoplasia were identified between May 2020 and June 2022. Patient demographic, initial presentation, histology, immunohistochemical, and in situ hybridization results were evaluated for each case.</div></div><div><h3>Results</h3><div>All cases presented in the ventral tongue and/or floor of mouth in adult males. One case was in an immunocompromised nonsmoker and the remaining three cases were in smokers. Immunohistochemistry for p16 was positive in all cases and high-risk HPV subtypes were identified in three cases by in situ hybridization. Histology showed full thickness, hyperplastic squamous dysplasia with hyperchromatic and pleomorphic nuclei, increased mitoses and apoptotic bodies, and variable keratosis with dyskeratosis in all cases.</div></div><div><h3>Conclusion</h3><div>Identification and diagnosis of this subset of oral epithelial dysplasia is facilitated by knowledge of how this entity presents itself, with immunocompromised adult males being the patient population at highest risk. Notably, the majority of cases illustrated here had an atypical presentation with a smoking history, which is not a commonly associated risk factor for HPV-driven lesions. Promotion of HPV vaccination by dental practitioners and early detection of these precursor lesions by healthcare professionals can lead to a decrease in oral cavity invasive squamous cell carcinoma.</div></div>","PeriodicalId":49010,"journal":{"name":"Oral Surgery Oral Medicine Oral Pathology Oral Radiology","volume":"140 3","pages":"Page e69"},"PeriodicalIF":2.0,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144672094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Information for Readers","authors":"","doi":"10.1016/S2212-4403(25)01105-8","DOIUrl":"10.1016/S2212-4403(25)01105-8","url":null,"abstract":"","PeriodicalId":49010,"journal":{"name":"Oral Surgery Oral Medicine Oral Pathology Oral Radiology","volume":"140 3","pages":"Page A6"},"PeriodicalIF":2.0,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144670423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recurrent glandular odontogenic cysts: a series of seven cases","authors":"Jeyda Turker ,&nbsp;Matthew Jenny ,&nbsp;Yingci Liu ,&nbsp;Raja R. Seethala ,&nbsp;Elizabeth Ann Bilodeau","doi":"10.1016/j.oooo.2025.04.062","DOIUrl":"10.1016/j.oooo.2025.04.062","url":null,"abstract":"<div><h3>Introduction</h3><div>We present a case series of recurrent glandular odontogenic cysts (rGOC), a rare, aggressive odontogenic cyst seen commonly in the anterior mandible of middle-aged women with reported recurrence rates of 22%. Our objective is to analyze the clinical and histological features of rGOCs from our institution.</div></div><div><h3>Materials and Methods</h3><div>The University of Pittsburgh Pathology archives were searched for rGOCs (1993-2022). A total of 265 glandular odontogenic cysts were diagnosed with a recurrence rate of 2.3%. We reviewed the slides and pathology reports for the clinicopathologic features of our recurrent cases and paired primary cases, when available.</div></div><div><h3>Results</h3><div>We assembled a series of six patients with seven rGOCs, with one patient having two recurrences. Average time to recurrence was 10.7 years (range: 2.3-22.6 years). With a mean age at initial diagnosis of 50.3 years (range: 24-69) and no sex predilection (M:F 1:1). All cases presented in the anterior gnathic region with an even jaw distribution (maxilla:mandible 1:1). Four tumors in two patients were <em>MAML2</em> translocation tested and negative, including one patient with two recurrences. Histologically, rGOCs had minimal inflammation and a flat epithelial connective tissue interface; separation at the epithelial-connective was noted in all cases, at least focally. Another interesting finding in the rGOCs was subepithelial hyalinization (epithelial inductive effect). All original and recurrent tumors reviewed demonstrated variable thickness, clear cells, and multiple compartments. In cases where both the original slides and recurrence were available for review, the recurrence lacked/lost on average 3.3 features. Microcysts were present in all primary cysts reviewed, but not seen in recurrent lesions.</div></div><div><h3>Conclusions</h3><div>In our single institution experience, rGOC is most common in the anterior gnathic region with lower recurrence rates than stated in the literature. Variable surgical management strategies are a confounding factor. Recurrent lesions histologically contained fewer characteristic GOC features than initial lesions.</div></div>","PeriodicalId":49010,"journal":{"name":"Oral Surgery Oral Medicine Oral Pathology Oral Radiology","volume":"140 3","pages":"Pages e86-e87"},"PeriodicalIF":2.0,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144670529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multicentric basal cell adenoma with intercalated Duct lesions","authors":"Allison Lee ,&nbsp;Paras Patel ,&nbsp;Victoria Woo ,&nbsp;Madhu Shrestha ,&nbsp;Arthur Bredeweg","doi":"10.1016/j.oooo.2025.04.056","DOIUrl":"10.1016/j.oooo.2025.04.056","url":null,"abstract":"<div><div>Intercalated duct lesions (IDLs) represent abnormal proliferations of the intercalated ducts that are occasionally associated with salivary gland neoplasms, such as epithelial-myoepithelial carcinoma and basal cell adenoma (BCA). The concomitant occurrence of these entities has led to speculation that IDLs may represent a precursor for certain salivary gland neoplasms. This is supported by recent studies that have demonstrated molecular similarities between IDLs and their associated tumors. Herein we describe a 65-year-old male who presented for evaluation of a left parotid mass present for 2 years. An MRI with contrast revealed 7 well-defined masses within the left parotid gland. The lesions demonstrated areas of heterogeneous enhancement with predominantly T2 hypointensity. The patient’s medical history was significant for an adenomatoid nodule of the left thyroid and a distant history of melanoma treated 40 years prior. Histologic examination of the left parotidectomy revealed multiple well-demarcated epithelial proliferations, in which tumor islands showed peripherally palisaded hyperchromatic nuclei and foci of ductal differentiation. Also noted within the peripheral parotid parenchyma were foci of intercalated ducts showing occasional serous acinar differentiation, consistent with IDLs. Immunohistochemical analysis revealed nuclear positivity for beta-catenin in both populations, and a Ki-67 showed a proliferative index of approximately 5%-10%. A diagnosis of multicentric basal cell adenoma with intercalated duct lesions was rendered. The patient is 1-month postexcision with no complications. The IDLs remain a poorly understood group of benign salivary gland proliferations that include hyperplasias, adenomas, and hybrid lesions displaying features of both. Their synchronous occurrence with benign salivary gland neoplasms can present diagnostic challenges and raise the possibility of malignancy if misinterpreted as infiltrative growth. A greater awareness of the histopathological and immunohistochemical spectrum of IDLs and their potential synchronous occurrence with salivary gland neoplasms can aid in avoiding such diagnostic pitfalls.</div></div>","PeriodicalId":49010,"journal":{"name":"Oral Surgery Oral Medicine Oral Pathology Oral Radiology","volume":"140 3","pages":"Pages e84-e85"},"PeriodicalIF":2.0,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144670557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unilateral cherubism: a case report and a review of the literature","authors":"Leigh Griffin,&nbsp;Carter Bruett,&nbsp;Paul Freedman,&nbsp;Reneé Reich","doi":"10.1016/j.oooo.2025.04.073","DOIUrl":"10.1016/j.oooo.2025.04.073","url":null,"abstract":"<div><div>An 8-year-old female presented to an oral surgeon with a referral from her pediatric dentist for evaluation of a radiolucency of her right posterior mandible. The patient’s parents reported no significant medical history for the patient, and she had no prior surgical history. She was not taking any medications or supplements. Review of the patient’s panoramic radiograph revealed a multilocular radiolucency of the right mandible posterior to an unerupted #31 and into the ascending-ramus. It appeared well-corticated and was composed of a few medium-sized locules. It appeared separate from the nearby developing tooth #31. The remainder of the panoramic radiograph was unremarkable, with appropriate tooth eruption patterns for the patient’s age and no noted tooth development or symmetric radiograph aberration at tooth site #17. On physical examination, the extraoral and intraoral soft tissues were unremarkable. No expansion was obvious and there were no overlying mucosal findings. The histopathologic features of the lesion were consistent with a giant cell lesion of cherubism. Giant cell lesions of the jawbones are common, but at times require clinical information in addition to the histopathology to render a specific diagnosis. In this case, the extensive eosinophilic cuffing of vessels and sparse hemorrhage suggested cherubism as a possible diagnosis. However, the unilateral presentation was considered unusual. The oral surgeon contacted the patient’s family and they were able to confirm a history of cherubism of the patient’s father. After the diagnosis, no further treatment was rendered. Management of cherubism involves watchful waiting as puberty often halts disease progression. In this case, there has been no progression to date. A review of the literature revealed that unilateral cherubism is rare. Only four cases of unilateral cherubism have been reported and of the four, two progressed to bilateral cherubism with continued monitoring.</div></div>","PeriodicalId":49010,"journal":{"name":"Oral Surgery Oral Medicine Oral Pathology Oral Radiology","volume":"140 3","pages":"Page e90"},"PeriodicalIF":2.0,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144670560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pilomatricoma: case report and review of the literature","authors":"Ian Mark ,&nbsp;Natasha Bhalla ,&nbsp;Joel Rosenfeld ,&nbsp;Jesse Doscher ,&nbsp;Christopher Lane","doi":"10.1016/j.oooo.2025.04.045","DOIUrl":"10.1016/j.oooo.2025.04.045","url":null,"abstract":"<div><h3>Introduction</h3><div>The etiology of a calcifying epithelioma might be due to a mutation in the <em>CTNNB1</em> gene. This gene codes for the beta-catenin protein and it plays a role in cell-cell adhesion at the cell membrane and signal transduction in the nucleus. This lesion was first described by Malharbe and Chenantais in 1880. It was renamed from calcifying epithelioma to pilomatricoma when the etiology was identified to arise from hair follicle matrix. Dermatological lesions are more common in children and young adults and more prevalent in the first decade of life. The most frequent location is the head and neck region. It usually manifests as a firm subcutaneous nodule of skin.</div></div><div><h3>Materials and Methods</h3><div>A 22-year-old female with no remarkable medical history presented with a 3-year history of well-demarcated, firm entity in the right preauricular region. The overlying skin was erythematous and exfoliating on exam. The entity was 3 cm x 2.5 cm. CT with contrast revealed a well-circumscribed oval-shaped 3.9 × 1.6 × 3.6 cm subcutaneous soft tissue tumor with densely packed punctate calcifications are seen involving the right temporal region anterior to the auricle. Clinical differential diagnosis included sialocele, lymphoepithelial cyst, and pleomorphic adenoma. Following the CT scan with contrast, the differential diagnosis was modified to a dermoid cyst, epidermoid cyst, and pilomatricoma due to superficial, subcutaneous nature of the pathology. An incisional biopsy was conducted under local anesthesia using a preauricular approach. Gross examination revealed a reddish brown with gritty hard tissue fragments.</div></div><div><h3>Results</h3><div>Based on the histopathological interpretation, the entity was diagnosed as a pilomatricoma. Conclusion: Pilomatricoma is an uncommon entity found in the head and neck region. Practitioners should be familiar with it and include in their differential diagnosis of cutaneous dermatopathology.</div></div>","PeriodicalId":49010,"journal":{"name":"Oral Surgery Oral Medicine Oral Pathology Oral Radiology","volume":"140 3","pages":"Page e81"},"PeriodicalIF":2.0,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144670654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral condyloma acuminatum: a clinicopathologic study of 25 cases","authors":"Kedkanya Mesil ,&nbsp;Sook-Bin Woo","doi":"10.1016/j.oooo.2025.04.069","DOIUrl":"10.1016/j.oooo.2025.04.069","url":null,"abstract":"<div><h3>Introduction</h3><div>Condyloma acuminatum (CondA) is a benign proliferative lesion of the squamous epithelium caused by the human papillomavirus (HPV), most often HPV-6 and -11. It presents as single or multiple papular or papillary lesions commonly found on anogenital skin and mucosa. CondA of the oral cavity was reported to mostly occur in sexually active men in the third and fourth decades. The objective of this study is to characterize the clinical and histopathologic features of a series of oral CondA.</div></div><div><h3>Materials and Methods</h3><div>Cases were identified from the archives of StrataDx Inc. from March 2020 to September 2022. Clinical data and histopathologic features were evaluated.</div></div><div><h3>Results</h3><div>There were 25 biopsies from 24 patients with 19 males (79.2%) and M:F of 3.8:1. The median age was 59 years (range 27-84 years); 76.0% of patients were 50 years or older. The most frequently affected site was the lower lip mucosa (28.0%); the ventral tongue, dorsal tongue, floor of mouth, and palatal mucosa were involved in 12.0% of cases each. CondA showed subtle or obvious papillary squamous epithelial hyperplasia with mild parakeratosis and bulbous rete ridges, arising from a sessile base. All cases exhibited cytologic atypia and intra-epithelial and stromal inflammation were noted in 32.0% and 52.0% of cases respectively. Apoptotic cells, karyorrhectic cells, and koilocytes were present in all cases and the mitotic count varied from 1 to 18 mitoses/10 high-power fields (median = 9 mitoses). All cases tested positive for HPV-6 and -11 by in situ hybridization.</div></div><div><h3>Conclusions</h3><div>Oral CondA mostly occurred in males generally in the sixth decade and older. It should be distinguished from other histologic mimics, including squamous papilloma but especially bluntly invasive squamous cell carcinoma and verrucous carcinoma because epithelial hyperplasia may be marked and there is generally significant cytologic atypia.</div></div>","PeriodicalId":49010,"journal":{"name":"Oral Surgery Oral Medicine Oral Pathology Oral Radiology","volume":"140 3","pages":"Page e89"},"PeriodicalIF":2.0,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144670386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extranodal non-Hodgkin’s lymphoma presented as bilateral enlargement of the mandibular canals: a case report","authors":"Rana Alshagroud ,&nbsp;Fahad Mutawwam ,&nbsp;Ahmed Alhumaidi ,&nbsp;Fadi Alakeel ,&nbsp;Farjah Algahtani","doi":"10.1016/j.oooo.2025.04.041","DOIUrl":"10.1016/j.oooo.2025.04.041","url":null,"abstract":"<div><h3>Introduction</h3><div>Bilateral enlargement of the mandibular canals (BEMC) is extremely rare and the differential diagnosis includes vascular malformation, benign neural tumors, or localized hypertrophic neuropathy. Extranodal non-Hodgkin’s lymphoma (ENHL) involving the mandibular canals is exceptionally rare with only five cases reported. Herein, we report an interesting case of ENHL presented as BEMCs.</div></div><div><h3>Materials and Methods</h3><div>A 43-year-old Saudi woman came to our oral surgery clinic complaining of heaviness in her mandible for 9 months. The panoramic radiograph revealed a bilateral diffuse uniform thickening of the mandibular canal. MRI showed isolated bilateral thickening and enhancement of mandibular branches of the trigeminal nerves with no involvement to the adjacent structures.</div></div><div><h3>Results</h3><div>An incisional biopsy was performed and microscopic examination showed a diffuse lymphocytic infiltration admixed with few plasma cells in a background of fibrosis. Neoplastic cells were positive for CD20 and PAX5 with Kappa chain restriction. Cyclin D1, IgG4, BCL6, and CD10 were negative. IgH rearrangement was positive for the clonal-B-cell receptor (IGH) gene.</div></div><div><h3>Conclusions</h3><div>We present an extremely unusual case of ENHL expanding the radiographic differential diagnosis of BEMC. More importantly, this was the first sign of primary ENHL. Thus, BEMC should rise the possibility of early malignancy which will allow early diagnosis and intervention.</div></div>","PeriodicalId":49010,"journal":{"name":"Oral Surgery Oral Medicine Oral Pathology Oral Radiology","volume":"140 3","pages":"Page e80"},"PeriodicalIF":2.0,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144670634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of ameloblastoma and odontogenic cysts with BRAFVE1 and CD56","authors":"Tharun Inturi,&nbsp;Rajaram Gopalakrishnan,&nbsp;Ioannis Koutlas","doi":"10.1016/j.oooo.2025.04.039","DOIUrl":"10.1016/j.oooo.2025.04.039","url":null,"abstract":"<div><h3>Introduction</h3><div>BRAFVE1 mutation-specific and CD56 antibodies have been proven to have diagnostic utility and expressed in ameloblastomas. Herein, we explored the utilization of these antibodies in cases of conventional (AMEL) and unicystic (UAMEL) ameloblastomas as well as other odontogenic cystic lesions including cysts that show features suggestive of unicystic ameloblastoma such as palisading basal cells and spongiosis without or minimal inflammation (QCYST).</div></div><div><h3>Methods and Materials</h3><div>Immunohistochemical analysis for BRAFVE1 and CD56 were performed in 23 AMEL; 9 unicystic AMEL (UAMEL), 6 QCYST, 8 dentigerous (DC), 5 radicular (RCYST) and one calcifying odontogenic cysts; 2 odontogenic keratocysts (OKC), 3 hyperplastic dental follicles (HDF) and 3 developing odontomas (ODONT). BRAFVE1 staining was determined as present or absent, while CD56 staining was assessed as negative, focal, or extensive.</div></div><div><h3>Results</h3><div>17/23 AMEL showed staining for both BRAFVE1 and CD56. CD56 (91.3%) performed superiorly compared to BRAFVE1 (73.9%). Only 1 of the 4 BRAFVE1 negative case affected the maxilla. Both antibodies were positive in 7/9 (77.8%) UAMEL cases. More interestingly, staining was observed in areas without the Vickers-Gorlin criteria. In lesions which were histologically diagnosed as DC but showed UAMEL features, both antibodies were negative in all cases but one showing focal CD56. Staining of basal and suprabasal cells was observed in OKCS. HDF and ODONT showed positive staining of included odontogenic rests with CD56. Finally, in the single COC, focal CD56 staining was observed. RCYST was negative for both antibodies.</div></div><div><h3>Conclusion</h3><div>BRAFVE1 and CD56 may disclose early ameloblastomatous changes not fulfilling the Vickers-Gorlin criteria in cystic odontogenic lesions. Both antibodies stain strongly in conventional ameloblastomas. Caution must be shown in using CD56 in other odontogenic lesions due to focal positive staining that could lead to diagnostic error.</div></div>","PeriodicalId":49010,"journal":{"name":"Oral Surgery Oral Medicine Oral Pathology Oral Radiology","volume":"140 3","pages":"Pages e79-e80"},"PeriodicalIF":2.0,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144670649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}