Journal of Proteome Research最新文献_第4页

Dynamic Atlas and Functional Regulatory Network of the Whole-Brain Proteome Across 24-Hour Temporal States in Vespertilio sinensis 中华狐猴全脑蛋白质组24小时时间状态的动态图谱和功能调控网络

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2026-10-02 Epub Date: 2026-09-14 DOI: 10.1021/acs.jproteome.6c00330

Tianhui Wang, Hui Wang, Xin Li, Jiang Feng

{"title":"Dynamic Atlas and Functional Regulatory Network of the Whole-Brain Proteome Across 24-Hour Temporal States in Vespertilio sinensis","authors":"Tianhui Wang, Hui Wang, Xin Li, Jiang Feng","doi":"10.1021/acs.jproteome.6c00330","DOIUrl":"https://doi.org/10.1021/acs.jproteome.6c00330","url":null,"abstract":"As the only mammals capable of powered flight, bats exhibitextrememetabolic fluctuations adapted to flight and a nocturnal lifestyle,making them unique models for studying diurnal rhythms and energyhomeostasis. We performed directDIA-based quantitative proteomicson whole-brain tissues of Vespertilio sinensis acrossfour distinct 24-hour physiological states: Rest, Sleep, Wake, andActivity. Among the 7652 identified proteins, a total of 643 differentiallyexpressed proteins (DEPs) were screened via pairwise comparisons acrosstimepoints. Time-series clustering further resolved two statisticallysignificant temporal expression modules (Module 3 and Module 9). Combinedwith functional enrichment of DEPs and phase set enrichment analysis(PSEA) of 574 rhythmic proteins, our multi-layered omics results collectivelyuncovered stage-specific molecular adaptive patterns. The Active stateupregulated oxidative phosphorylation and thermogenesis for high energydemands, the Rest state activated immune clearance and autophagy toeliminate flight-induced metabolic damage, the Sleep state suppressedglobal transcription, calcium signaling and DNA repair to reduce neuralenergy consumption, and the Wake state (pre-dusk) pre-activated sulfurbiosynthesis, antioxidant defense, and energy metabolic pathways toprepare for upcoming nocturnal activity. Parallel transcriptomic andproteomic rhythmic analysis further identified 19 conserved oscillatorymolecules at both molecular layers, revealing partial transcript-proteinrhythmic decoupling in the bat brain and refining the diurnal regulatorylandscape. As the first systematic atlas of the bat whole-brain proteomeacross a 24-hour cycle, this study uncovers molecular strategies maintainingbrain homeostasis, providing a foundation for understanding diurnalphysiological adaptation, flight energy regulation, and circadianoutput pathways.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":"25 10","pages":"5376–5390"},"PeriodicalIF":3.8,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149124742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Top-Down Proteomics Analysis of pg-Level Cell Lysate Using Spray-Capillary CE-FAIMS-MS 使用喷雾-毛细管CE-FAIMS-MS对pg水平细胞裂解液进行自上而下的蛋白质组学分析

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2026-10-02 Epub Date: 2026-09-16 DOI: 10.1021/acs.jproteome.6c00353

Samin Anjum, Zhitao Zhao, Kellye A. Cupp-Sutton, Si Wu

{"title":"Top-Down Proteomics Analysis of pg-Level Cell Lysate Using Spray-Capillary CE-FAIMS-MS","authors":"Samin Anjum, Zhitao Zhao, Kellye A. Cupp-Sutton, Si Wu","doi":"10.1021/acs.jproteome.6c00353","DOIUrl":"https://doi.org/10.1021/acs.jproteome.6c00353","url":null,"abstract":"Capillary electrophoresis-massspectrometry (CE-MS) isa promisingtechnique for ultrasensitive top-down proteomics due to its high resolutionand sensitivity compared with traditional liquid chromatography (LC)-basedmethods. However, CE-MS-based top-down proteomics of mass-limitedsamples remains challenging because of the need to handle ultralowsample amounts. Recently, we developed a spray-capillary CE-MS platformfor ultrasensitive, low-volume top-down proteomics analysis, allowingfor the detection of hundreds of intact proteoforms from pg-levelsamples. Here, we integrated this platform with high-field asymmetricwaveform ion mobility spectrometry (FAIMS) to enhance proteoform coveragein mass-limited samples (i.e., samples with limited input mass). Ourresults suggested that FAIMS effectively enhanced the signal-to-noiseratio for pg-level samples by filtering background noise. At low injectionmasses (e.g., 1 pg), experiments performed with FAIMS yielded higherrelative abundance than those conducted without FAIMS. Interestingly,in all cases, we did not observe the substantial loss in intensitycommonly reported for bottom-up proteomics with FAIMS. Additionally,we implemented 2D CE-FAIMS-MS by applying five different FAIMS compensationvoltages (CVs) for gas-phase intact proteoform fractionation. Thisplatform detected >3000 yeast proteoforms using 100 pg per CV.Overall,our results suggested that the spray-capillary CE-FAIMS-MS platformenables deep proteoform characterization of pg-level samples and holdspotential for analyzing intact proteoforms in mass-limited biologicalsamples.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":"25 10","pages":"5430–5439"},"PeriodicalIF":3.8,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149124809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cross-Platform Evaluation Reveals Sample Preparation-Driven Biological Biases despite Advances in Mass Spectrometry Performance in Serum Proteomics 跨平台评估揭示了样品制备驱动的生物学偏差，尽管在血清蛋白质组学的质谱性能方面取得了进展

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2026-10-02 Epub Date: 2026-09-10 DOI: 10.1021/acs.jproteome.6c00493

Drosos Katsavelis, Marieke G. C. van der Hart, Justina C. Wolters, Hjalmar P. Permentier, Peter L. Horvatovich, Thomas I.F.H. Cremers

{"title":"Cross-Platform Evaluation Reveals Sample Preparation-Driven Biological Biases despite Advances in Mass Spectrometry Performance in Serum Proteomics","authors":"Drosos Katsavelis, Marieke G. C. van der Hart, Justina C. Wolters, Hjalmar P. Permentier, Peter L. Horvatovich, Thomas I.F.H. Cremers","doi":"10.1021/acs.jproteome.6c00493","DOIUrl":"https://doi.org/10.1021/acs.jproteome.6c00493","url":null,"abstract":"Advances in mass spectrometry (MS) instrumentation andproteinextraction technologies are reshaping proteomics; however, whetherimproved analytical performance alters the biological informationaccessible from different sample preparation strategies remains unclear.Here, we performed a cross-platform evaluation using identical benchmarkserum samples previously analyzed on an Orbitrap Exploris 480 platform,including the newly introduced Seer Proteograph ONE workflow, to determinehow MS advances and sample preparation influence analytical performanceand biological interpretation. Across all preparation strategies,the timsTOF HT improved analytical performance by increasing proteinand peptide identifications, reducing missing values, and improvinglinearity in spike-in experiments, particularly for low-abundanceproteins. Despite these improvements, sample preparation remainedthe major determinant of the accessible serum proteome. Functionalenrichment analysis revealed reproducible workflow-specific biasesacross MS platforms: nanoparticle-based enrichment expanded accessto a broader range of serum proteins and improved detection of clinicallyrelevant signaling-associated proteins, including cytokine- and chemokine-relatedmarker classes, whereas the depletion-based strategy showed a distinctfunctional profile enriched for receptor-related activities. Together,these findings demonstrate that MS advances enhance analytical performancebut do not eliminate sample preparation-driven differences in biologicalaccessibility. This study provides a framework for selecting serumproteomics workflows according to specific biomarker and pathway objectives.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":"25 10","pages":"5480–5492"},"PeriodicalIF":3.8,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149093544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MetaproDB: A Flexible and Reproducible Framework for Biome-Informed Protein Sequence Database Construction for Metaproteomics MetaproDB：一个灵活和可复制的b宏蛋白质组学生物群系信息蛋白序列数据库构建框架

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2026-10-02 Epub Date: 2026-09-16 DOI: 10.1021/acs.jproteome.6c00326

Muzaffer Arıkan

引用次数: 0

Evaluation of Deep and Dynamic Proteomic Screening Strategies at Sub-50 Hz Scan Speed and without Robotic Sample Preparation 在低于50赫兹的扫描速度下，无机器人样品制备的深度和动态蛋白质组学筛选策略的评估

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2026-10-02 Epub Date: 2026-09-15 DOI: 10.1021/acs.jproteome.6c00312

Bhavesh S. Parmar, Yuanyuan Liu, Parviz Ghezellou, Christian Münch

{"title":"Evaluation of Deep and Dynamic Proteomic Screening Strategies at Sub-50 Hz Scan Speed and without Robotic Sample Preparation","authors":"Bhavesh S. Parmar, Yuanyuan Liu, Parviz Ghezellou, Christian Münch","doi":"10.1021/acs.jproteome.6c00312","DOIUrl":"https://doi.org/10.1021/acs.jproteome.6c00312","url":null,"abstract":"Advances in ultrafastmass analyzer technology and procedural automationhave enabled proteomic screening at the throughput of hundreds ofproteomes per day. However, these approaches often require expensiveinstrumentation upgrades and robotic automation that remain inaccessibleto many research laboratories and core facilities. In this study,we address the feasibility of scaling up proteomic screening capabilitieswith minimal upgrade cost by focusing on (a) strategies for nonautomatedhigh-throughput sample preparation from 96-well cell culture, (b)data acquisition on sub-50 Hz scan speed hybrid and tribrid Orbitrapinstruments, and (c) data analysis strategies for label-free and labeledproteomic screening. We find that the 96-well format STrap, in combinationwith C18 plates, provides the most robust throughput for a nonautomatedsample preparation workflow. Furthermore, we show that for staticproteomes, an isobaric tandem mass tag (TMT)-based multiplexing approachprovides deeper and more precise proteome coverage, whereas data-independentacquisition (DIA) is more accurate, albeit with a reduced dynamicrange and more missing values. Finally, we extend the optimized workflowto proteome turnover studies using pulsed stable isotope labelingby amino acids in cell culture (pSILAC), highlighting the key advantagesand trade-offs of DIA and TMT data-dependent acquisition strategiesfor capturing protein translation.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":"25 10","pages":"5255–5266"},"PeriodicalIF":3.8,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149124814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Proteomic Profiling of Inner Ear and Systemic Fluids Following Noise-Induced Hearing Loss in Rats 噪声性听力损失后大鼠内耳和全身液体的综合蛋白质组学分析

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2026-10-02 Epub Date: 2026-09-14 DOI: 10.1021/acs.jproteome.6c00316

Motahare Khorrami, Zeshan Ali, Robert D. Gay, Ya Lang Enke, Mohsen Asadnia, Paul A. Haynes, Christopher Pastras

{"title":"Integrated Proteomic Profiling of Inner Ear and Systemic Fluids Following Noise-Induced Hearing Loss in Rats","authors":"Motahare Khorrami, Zeshan Ali, Robert D. Gay, Ya Lang Enke, Mohsen Asadnia, Paul A. Haynes, Christopher Pastras","doi":"10.1021/acs.jproteome.6c00316","DOIUrl":"https://doi.org/10.1021/acs.jproteome.6c00316","url":null,"abstract":"Noise-inducedhearing loss (NIHL) arises from acute or prolongedexposure to loud sounds, yet early diagnosis remains difficult assome individuals show minimal changes in standard audiometry. Thisstudy presents the first comparative mass spectrometry-based proteomicanalysis of perilymph, cochlear-derived cerebrospinal fluid (CSF),whole blood, and plasma in Sprague–Dawley rats following acutenoise exposure, to identify molecular signatures of NIHL. Rats wereexposed to 110 dB noise for 1 h under anesthesia, inducing an approximately40 dB threshold shift measured by cochlear nerve Compound Action Potential(CAP). Nano LC–MS/MS in data-independent acquisition (DIA)mode followed by peptide- and protein-level filtering, identified310 proteins in perilymph, 396 in CSF, 1688 in blood, and 281 in plasma.Differential expression analysis revealed 50, 79, 281, and 49 significantlychanged proteins, respectively. A shared inflammatory response wasobserved, highlighted by consistent upregulation of IGHM across allfluids. Cochlear samples showed enrichment of metabolic and injury-relatedproteins, including PGAM2 and ALDOA, while systemic samples exhibitedchanges in fibrinogen chains, IGFBP5, and apolipoproteins. KEGG pathwayanalysis indicated involvement of metabolic, immune, and lipid-regulatorypathways. Targeted PRM validation confirmed selected protein changes.These findings demonstrate coordinated local and systemic responsesto noise trauma, supporting the potential of peripheral biomarkersfor early NIHL detection.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":"25 10","pages":"5267–5283"},"PeriodicalIF":3.8,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149124746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amino Acid Substitutomics: Profiling Amino Acid Substitutions at the Proteomic Scale Unveils Biological Implications and Escape Hypotheses in Cancer 氨基酸取代组学：分析蛋白质组学尺度上的氨基酸取代揭示了癌症的生物学意义和逃避假说

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2026-10-02 Epub Date: 2026-08-27 DOI: 10.1021/acs.jproteome.6c00455

Peize Zhao, Shengzhi Lai, Shuaijian Dai, Chen Zhou, Ning Li, Weichuan Yu

{"title":"Amino Acid Substitutomics: Profiling Amino Acid Substitutions at the Proteomic Scale Unveils Biological Implications and Escape Hypotheses in Cancer","authors":"Peize Zhao, Shengzhi Lai, Shuaijian Dai, Chen Zhou, Ning Li, Weichuan Yu","doi":"10.1021/acs.jproteome.6c00455","DOIUrl":"https://doi.org/10.1021/acs.jproteome.6c00455","url":null,"abstract":"Amino acid (AA) substitutionsare pivotal modulators of cellularsignaling and homeostasis. Current studies of AA substitutions relymainly on genomic and transcriptomic evidence, while proteome-levelcharacterization remains underexplored, even though such substitutionscan arise beyond DNA and RNA mutations. Here we address this gap withPIPI-C, an open-search mass spectrometry tool, and establish an integratedAA substitutomics pipeline to profile AA substitutions across fivecancer cohorts and dissect the regulatory roles of substitution-bearingproteins. By cross-referencing 13,399 identified AA substitutionswith matched whole-exome sequencing data, we observed no overlap withpatient somatic mutations. Specifically, 61% of these substitutionsrequired two or more base alterations and could not be derived fromsingle-nucleotide variants, rendering them exclusively detectableat the proteomic level. The remaining 39% of substitutions were compatiblewith single-base variations. PIPI-C captured these candidate substitutionsat the proteome scale, consistent with a translational-stage originas reported for alternate RNA decoding in mammals. We further identifiednotable substitutions, including F43S and E91D in hemoglobin subunitbeta, P584T in filamin A, and A175N in fructose-bisphosphate aldolaseB, and our pipeline provides a framework for generating hypothesesabout AA substitutions in cancer escape.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":"25 10","pages":"5452–5468"},"PeriodicalIF":3.8,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149093582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Semaglutide and Tirzepatide Suppress Hepatic Fatty Acid Uptake and Fatty Acid β-Oxidation in High-Fat Diet-Induced Obesity Mice 西马鲁肽和替西帕肽抑制高脂饮食诱导的肥胖小鼠肝脏脂肪酸摄取和脂肪酸β-氧化

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2026-10-02 Epub Date: 2026-09-13 DOI: 10.1021/acs.jproteome.6c00404

Dhavalkumar Patel, Heba Ewida, Syed Tareq, Md Tareq Aziz, Prasanth K. Chelikani, Ulrich Bickel, Mahmoud Salama Ahmed

{"title":"Semaglutide and Tirzepatide Suppress Hepatic Fatty Acid Uptake and Fatty Acid β-Oxidation in High-Fat Diet-Induced Obesity Mice","authors":"Dhavalkumar Patel, Heba Ewida, Syed Tareq, Md Tareq Aziz, Prasanth K. Chelikani, Ulrich Bickel, Mahmoud Salama Ahmed","doi":"10.1021/acs.jproteome.6c00404","DOIUrl":"https://doi.org/10.1021/acs.jproteome.6c00404","url":null,"abstract":"Glucagon-like peptide-1 (GLP-1) and dual GLP-1/glucose-dependentinsulinotropic polypeptide (GIP) receptor agonists show high efficacyin treating obesity and metabolic dysfunction-associated liver diseases,conditions that collectively represent one of the most challengingOne Health crises of our time. However, their hepatic molecular mechanismsremain poorly understood. This study defined targeted hepatic proteomicsignatures associated semaglutide (GLP-1 agonist) and tirzepatide(dual GLP-1/GIP) in diet-induced obese (DIO) male C57BL/6J mice usingan liquid chromatography-tandem mass spectrometry (LC-MS/MS)-basedtargeted-proteomics approach and validated using western blotting.After 18 weeks of high-fat diet (HFD), obese mice received once-dailysubcutaneous semaglutide (10 nmol/kg), tirzepatide (3 nmol/kg), orvehicle for 4 weeks. Tirzepatide produced greater systemic efficacy(24.6% body weight and 30.3% fat mass reduction, preserved lean mass,improved glucose homeostasis), yet both drugs yielded overlappingtargeted hepatic proteomic signatures despite different receptor pharmacology.Both drugs significantly downregulated CD36 and CPT1A fatty acid importersfor β-oxidation without altering lipogenic enzymes (FASN, ACC1,ACLY, ACSS2) or glucose-handling proteins (PDH, GLUT2). These findingssuggest a substrate limitation over enhanced β-oxidation orbroad lipogenesis-suppression, suggesting GLP-1 receptor activationis sufficient for the core liver response and GIP co-agonism mainlyaugments extrahepatic effects. From a One Health proteomics perspective,CD36/CPT1A anchored targeted proteomic signature provides a mechanisticallyinformed benchmark for evaluating novel GLP-1 and non-GLP-1 investigationaltherapeutics.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":"25 10","pages":"5418–5429"},"PeriodicalIF":3.8,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149093664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proximity Labeling of D1-Like Dopamine Receptors Captures Distinct Cellular Environments and Uncovers Trafficking Proteins That Regulate DA-Mediated Behaviors in Drosophila d1样多巴胺受体的接近标记捕获不同的细胞环境并揭示了调节果蝇da介导行为的运输蛋白

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2026-10-02 Epub Date: 2026-09-14 DOI: 10.1021/acs.jproteome.6c00259

Dana C. Guhle, Bhavana Kanagala, Rebecca Dust, Ross Evashkevich, Ronald L. Davis, Jacob A. Berry

{"title":"Proximity Labeling of D1-Like Dopamine Receptors Captures Distinct Cellular Environments and Uncovers Trafficking Proteins That Regulate DA-Mediated Behaviors in Drosophila","authors":"Dana C. Guhle, Bhavana Kanagala, Rebecca Dust, Ross Evashkevich, Ronald L. Davis, Jacob A. Berry","doi":"10.1021/acs.jproteome.6c00259","DOIUrl":"https://doi.org/10.1021/acs.jproteome.6c00259","url":null,"abstract":"The neurotransmitter dopamine (DA) is central to synapticregulationthat supports diverse behavioral functions, including both learningand forgetting. This multifunctional role of DA is due to receptor-specificsignaling in specific subcellular environments that remain uncharacterized.Here we used Turbo-mediated proximity labeling in human cells to characterizethe proximal environments of two Drosophila D1-likeDA receptors (Dop1R1 and Dop1R2) in basal and DA-activation environments.DA drives both Dop1R1-Turbo and Dop1R2-Turbo to recruit β-arrestin2, and Dop1R1-Turbo showed ligand-driven proximity to G-protein receptorkinase 3, members of clathrin-mediated endocytosis pathways, and WASHcomplex-mediated endosomal trafficking pathways. Additionally, weshow evidence that Dop1R1 and Dop1R2 reside in distinct domains onthe cell surface. In vivo disruption of Drosophila orthologues of Dop1R proximal proteins revealed three traffickingproteins, Sec24AB, Krz (beta-arrestin 2), and CG13887, that regulateR1-mediated learning, starvation-induced attraction to odors, andDA-mediated cAMP responses in memory circuits. In addition to revealingDA receptor trafficking proteins that support learning, our comparativecharacterization of the cellular environments of D1-like receptorsoffers insights into how DA differentially regulates diverse behavioraland synaptic functions.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":"25 10","pages":"5339–5355"},"PeriodicalIF":3.8,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149093626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sexual Dimorphism and Intraspecific Toxin Variation in Crotalus durissus terrificus Venom: Insights from Proteomics, Peptidomics, and Structural Modeling 鸡爪蟾毒液的两性异形和种内毒素变异：来自蛋白质组学、肽组学和结构模型的见解。

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2026-10-02 Epub Date: 2026-09-04 DOI: 10.1021/acs.jproteome.6c00275

Jackson G. Miyamoto, Jackelinne Y. Hayashi, Giuseppe G. Figueirêdo Leite, Sávio S. Sant’Anna, Hang Fai Kwok, Richard H. Valente, Anita M. Tanaka-Azevedo, Alexandre K. Tashima

{"title":"Sexual Dimorphism and Intraspecific Toxin Variation in Crotalus durissus terrificus Venom: Insights from Proteomics, Peptidomics, and Structural Modeling","authors":"Jackson G. Miyamoto, Jackelinne Y. Hayashi, Giuseppe G. Figueirêdo Leite, Sávio S. Sant’Anna, Hang Fai Kwok, Richard H. Valente, Anita M. Tanaka-Azevedo, Alexandre K. Tashima","doi":"10.1021/acs.jproteome.6c00275","DOIUrl":"10.1021/acs.jproteome.6c00275","url":null,"abstract":"The venom of the South American rattlesnake Crotalus durissus terrificus (Cdt) is renowned for its minimalist yet highlytoxic arsenal. Intraspecific variability in expressed proteoformsand toxin content has been previously observed in Cdt venom, resultingin heterogeneous biological activities. In light of this, our studyconducted an in-depth mass spectrometry-based analysis, combined withstructural prediction to comprehensively assess Cdt venom variabilityand sexual dimorphism. Using peptidomic analysis, we identified 76native peptides within Cdt venom, predominantly (63%) associated withnoncanonical processing of crotoxin subunits, and 9 novel peptidesvia de novo sequencing. Quantitative analysis showedincreased abundance of two crotapotin-derived peptides in females,while three novel peptides were significantly increased in males.Through quantitative proteomics, we characterized a repertoire of33 proteins, with over one-third displaying sex-based differencesin abundance. Furthermore, variant analysis revealed 12 novel toxinvariants, generally at lower abundance than previously described proteoforms.Predictive three-dimensional structure modeling revealed conservedscaffolds, suggesting preservation of their biological activities.Addressing venom variability provides valuable insights into the intricatevariability of Cdt venom, contributing to the understanding of itsbiological, medical, and biotechnological implications.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":"25 10","pages":"5230–5245"},"PeriodicalIF":3.8,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148890387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0