Journal of Proteome Research最新文献_第5页

Proteomic and Physicochemical Analysis of Erythrocyte Membrane Vesicles from Fresh and Frozen Blood 新鲜和冷冻血液中红细胞膜小泡的蛋白质组学和理化分析

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2026-10-02 Epub Date: 2026-09-09 DOI: 10.1021/acs.jproteome.6c00535

Katarina Tomić, Sara Ivanovski, Jernej Šribar, Daša Čebulj, Samuel Žvanut, Metka Lenassi, Marko Fonović, Igor Križaj, Nina Kostevšek

{"title":"Proteomic and Physicochemical Analysis of Erythrocyte Membrane Vesicles from Fresh and Frozen Blood","authors":"Katarina Tomić, Sara Ivanovski, Jernej Šribar, Daša Čebulj, Samuel Žvanut, Metka Lenassi, Marko Fonović, Igor Križaj, Nina Kostevšek","doi":"10.1021/acs.jproteome.6c00535","DOIUrl":"https://doi.org/10.1021/acs.jproteome.6c00535","url":null,"abstract":"Erythrocyte membranevesicles (EMVs) have emerged as promisingnanocarriers for drug delivery due to their biocompatibility and immuneevasion through retention of erythrocyte surface proteins. The mainlimitation is their dependence on freshly drawn blood, which limitsbatch production. Long-term storage of EDTA-anticoagulated blood at−80°C without cryoprotectant represents an accessiblealternative, but its effect on EMV physicochemical properties andprotein composition has not been investigated. We prepared EMVs fromfresh and frozen blood and characterized the resulting fractions bydynamic light scattering, nano-flow cytometry, cryo-transmission electronmicroscopy, negative staining transmission electron microscopy, andquantitative mass spectrometry-based proteomics. EMV size, surfacecharge, and morphology did not differ significantly between freshand frozen preparations. Extrusion effectively normalized particlesize despite increased heterogeneity in frozen ghost cell fractions,and both preparations yielded structurally intact vesicles after ultracentrifugation.At the proteomic level, freezing reduced proteomic complexity in membranefractions and selectively depleted cytosol-associated proteins. Thecore membrane proteome, including Band 3, remained stable. However,glycophorin A showed the largest reduction in frozen preparations,and CD47 levels were reduced in the ultracentrifuged fraction. Thesedifferences may be critical for applications where preservation ofspecific surface proteins is required, such as immune evasion or targeteddrug delivery. In some cases, frozen blood is potentially a viablesource for EMV production when immediate processing is not feasible.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":"25 10","pages":"5534–5548"},"PeriodicalIF":3.8,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149093542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Urinary Proteomic Panel Distinguishes Acute Myocarditis from Acute Myocardial Infarction 尿蛋白组学小组区分急性心肌炎和急性心肌梗死

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2026-10-02 Epub Date: 2026-09-03 DOI: 10.1021/acs.jproteome.6c00106

Yufei Wang, Yuhan Qin, Jing Yang, Qinghai Wang, Aiwei Wang, Jing Zhang, Anhu Wu, Xiaohan Qin, Jiaqi Yu, Zhenyu Liu, Xiaoxiao Guo

{"title":"Urinary Proteomic Panel Distinguishes Acute Myocarditis from Acute Myocardial Infarction","authors":"Yufei Wang, Yuhan Qin, Jing Yang, Qinghai Wang, Aiwei Wang, Jing Zhang, Anhu Wu, Xiaohan Qin, Jiaqi Yu, Zhenyu Liu, Xiaoxiao Guo","doi":"10.1021/acs.jproteome.6c00106","DOIUrl":"https://doi.org/10.1021/acs.jproteome.6c00106","url":null,"abstract":"Reliablenoninvasive discrimination of acute myocarditis(AM) fromacute myocardial infarction (AMI) in patients presenting with acutechest pain remains an unmet clinical need. We aimed to identify urinaryproteomic markers using mass spectrometry and LASSO regression. Patientswith suspected AM or AMI were prospectively enrolled. Urinary proteomeswere profiled by data-independent acquisition mass spectrometry. Thediscovery cohort was stratified by age and sex and randomly dividedinto training and internal validation sets at a 3:1 ratio. Differentiallyexpressed proteins were screened, and a diagnostic model was constructedusing LASSO with 1000 bootstrap resamplings in the training set. Themodel was validated internally and further verified in an independentcohort using ELISA. A total of 42 AM and 80 AMI patients were includedin the discovery cohort. Differential expression analysis in the trainingset identified 880 and 1099 differentially expressed proteins in AMand AMI, respectively, compared with controls. A three-protein panelcomprising TNFRSF1A, F9, and PRDX3 achieved an AUC of 0.954 in thetraining set and exceeded the clinical models (all DeLong’s P < 0.05). Addition of the protein panel to either clinicalmodel further improved diagnostic performance (both P < 0.001). Similar performance was confirmed in the validationcohorts. A urinary proteomic panel comprising TNFRSF1A, F9, and PRDX3may provide a noninvasive approach for differentiating AM from AMI.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":"25 10","pages":"5189–5199"},"PeriodicalIF":3.8,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149093642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating Metabolomics and Machine Learning Reveals Metabolic Signatures and Candidate Biomarkers for Vestibular Migraine 整合代谢组学和机器学习揭示前庭偏头痛的代谢特征和候选生物标志物

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2026-10-02 Epub Date: 2026-09-07 DOI: 10.1021/acs.jproteome.6c00143

Hongliang Xu, Qingyue Yuan, Xin Chen, He Fang, Haifeng Zhang, Liguo Zhang, Yun Yuan, Yajun Lian, Yikang Wang

{"title":"Integrating Metabolomics and Machine Learning Reveals Metabolic Signatures and Candidate Biomarkers for Vestibular Migraine","authors":"Hongliang Xu, Qingyue Yuan, Xin Chen, He Fang, Haifeng Zhang, Liguo Zhang, Yun Yuan, Yajun Lian, Yikang Wang","doi":"10.1021/acs.jproteome.6c00143","DOIUrl":"https://doi.org/10.1021/acs.jproteome.6c00143","url":null,"abstract":"Vestibular migraine (VM) is an underrecognized disordercharacterizedby episodic vertigo, often accompanied by headache. Despite its substantialprevalence and disease burden, its metabolic features remain poorlycharacterized, and serum biomarkers for diagnosis or activity assessmentare lacking. We performed untargeted metabolomics in discovery (33VM vs 30 healthy controls [HCs]) and replication (37 VM vs 27 HCs)cohorts, applying machine learning solely in the discovery cohortfor marker selection. In the discovery cohort, 510 metabolites exhibiteddifferential abundance (197 upregulated, 313 downregulated). KyotoEncyclopedia of Genes and Genomes (KEGG) enrichment analysis revealedenrichment trends for upregulated metabolites in cysteine/methionine,histidine, and phenylalanine metabolism. Machine learning identifiedthree candidate metabolites─phenylethylamine, N-acetylserotonin, and cysteine-S-sulfate─each achieving anarea under the curve > 0.9. In the discovery cohort, cysteine-S-sulfatecorrelated with vertigo Visual Analog Scale scores, and phenylethylaminecorrelated with Headache Impact Test-6 and Generalized Anxiety Disorder-7(GAD-7) scores. In the replication cohort, all three markers maintaineddifferential expression and discriminative performance; additionally,cysteine-S-sulfate correlated with Dizziness Handicap Inventory scores,and phenylethylamine with GAD-7 scores. This study elucidates themetabolic landscape of VM and identifies promising serum biomarkers,though these findings warrant further validation in larger, multicentercohorts before clinical translation.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":"25 10","pages":"5146–5158"},"PeriodicalIF":3.8,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149124858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-Omics Network Integration Reveals Cross-Domain Molecular Correlates of Fetal Bovine Serum Grade 多组学网络整合揭示胎牛血清等级的跨域分子相关性

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2026-10-02 Epub Date: 2026-09-08 DOI: 10.1021/acs.jproteome.6c00372

Akila Wijerathna-Yapa, Arslan Hamid, Stanislav Sokolenko

{"title":"Multi-Omics Network Integration Reveals Cross-Domain Molecular Correlates of Fetal Bovine Serum Grade","authors":"Akila Wijerathna-Yapa, Arslan Hamid, Stanislav Sokolenko","doi":"10.1021/acs.jproteome.6c00372","DOIUrl":"https://doi.org/10.1021/acs.jproteome.6c00372","url":null,"abstract":"Fetal bovine serum (FBS) is the dominantsupplement in mammaliancell culture, yet its quality grades are typically treated as fixed,defined inputs despite a poorly characterized molecular basis. Wepresent a multi-omics meta-analysis integrating original proteomics,peptidomics, metabolomics, and lipidomics datasets from two companionstudies across three commercial FBS grades (Low, Medium, High), usingMOFA2, DIABLO, cross-omics correlation networks, and WGCNA. Integratedmulti-omics embeddings achieved markedly superior grade separation(silhouette score 0.98) versus any single omics layer (0.80–0.87).MOFA2 identified a shared proteomics–metabolomics–peptidomicsquality axis, while ceramides and sphingolipids emerged as centralcross-omics hubs, and HERC2P9-derived peptides and diethylstilbestrolas grade-discriminating features. Because each grade was representedby a single commercial lot, grade and batch are confounded here, andthe reported differences should be read as lot-associated, pendingmulti-lot validation. Rather than certifying FBS grade as a fixedmolecular category, this study is intended as an open resource: itdemonstrates the depth of coordinated molecular variation across commercialFBS products and provides a reproducible multi-omics framework, withall raw data and analysis code openly deposited, for researchers tocharacterize their own serum lots before biologically sensitive work,rather than assuming compositional equivalence from a grade labelalone.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":"25 10","pages":"5356–5375"},"PeriodicalIF":3.8,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149093604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

2-Mercaptoethanol/DMSO Workflow Enables Highly Reproducible Quantitative Proteomics 2-巯基乙醇/DMSO工作流程实现高度可重复的定量蛋白质组学。

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2026-09-04 Epub Date: 2026-05-08 DOI: 10.1021/acs.jproteome.6c00010

Arisa Suto, Yoshihiro Ishikawa, Toshihide Matsumoto, Makoto Itakura, Yoshio Kodera, Takashi Matsui

{"title":"2-Mercaptoethanol/DMSO Workflow Enables Highly Reproducible Quantitative Proteomics","authors":"Arisa Suto, Yoshihiro Ishikawa, Toshihide Matsumoto, Makoto Itakura, Yoshio Kodera, Takashi Matsui","doi":"10.1021/acs.jproteome.6c00010","DOIUrl":"10.1021/acs.jproteome.6c00010","url":null,"abstract":"Proteomicsprovides a systematic and high-throughput approach tocomprehensively characterize protein networks, enabling insights intocellular functions and disease mechanisms. Carbamidomethylation usingiodoacetamide (IAA), a common method for cysteine alkylation, is knownto cause nonspecific modifications that increase spectral complexityin mass spectrometry and reduce quantitative accuracy. Here, we establisheda reproducibility-focused 2-mercaptoethanol (2-ME)/dimethyl sulfoxide(DMSO) workflow and systematically evaluated its quantitative performanceat the proteome-wide level. Mouse liver proteomes were processed usingeither 2-ME/DMSO or conventional IAA treatment, followed by liquidchromatography-tandem mass spectrometry (LC–MS/MS) analysis.The optimized 2-ME treatment increased the number of cysteine-modifiedpeptides by 1.6- to 1.9-fold. Although total protein identificationswere comparable, 77% of proteins exhibited improved sequence coveragewith the optimized 2-ME treatment. Quantitative reproducibility wasalso enhanced, with the peptide quantified CV ≤ 20% increasingfrom 61.4% with IAA treatment to 86.1% with 2-ME treatment, and proteinquantified CV ≤ 20% increasing from 80.6% with IAA treatmentto 93.5% with 2-ME treatment. Application of this new workflow toovarian clear cell carcinoma reliably detected cisplatin-induced alterations.The 2-ME/DMSO workflow offers a simple and highly reproducible proteomicsstrategy for accurate quantitative proteomics.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":"25 9","pages":"4524–4533"},"PeriodicalIF":3.8,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147830749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Omics Profiling of Patients with Obstructive Sleep Apnoea Reveals Risks of Diabetes Mellitus and Cardiovascular Diseases 阻塞性睡眠呼吸暂停患者的组学分析揭示了糖尿病和心血管疾病的风险

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2026-09-04 Epub Date: 2026-08-06 DOI: 10.1021/acs.jproteome.5c00919

Zhiling Zhao, Hanyujie Kang, Xiahui Li, Yafei Wang, Xiaotao Wu, Junqing Liu, Jing Li, Ziting Liang, Lujia Guan, Zhenfang Shan, Jieqiong Li, Zhaohui Tong

{"title":"Omics Profiling of Patients with Obstructive Sleep Apnoea Reveals Risks of Diabetes Mellitus and Cardiovascular Diseases","authors":"Zhiling Zhao, Hanyujie Kang, Xiahui Li, Yafei Wang, Xiaotao Wu, Junqing Liu, Jing Li, Ziting Liang, Lujia Guan, Zhenfang Shan, Jieqiong Li, Zhaohui Tong","doi":"10.1021/acs.jproteome.5c00919","DOIUrl":"10.1021/acs.jproteome.5c00919","url":null,"abstract":"Obstructivesleep apnoea (OSA) constitutes a multisystemic disorderoften associated with cardiovascular and metabolic disorders. Thus,far, the underlying pathophysiological processes are not fully understood.In total, 142 plasma samples were acquired: 50 from controls (CON),45 from mild/moderate OSA (M-OSA) patients, and 47 from severe OSA(S-OSA) patients. Proteomic and metabolic signatures significantlydiffered among S-OSA, M-OSA, and CON samples. A novel plasma biomarkerpanel including two proteins (ACTR2 and ENO1) and three metabolites(2-aminobicyclo[2 2·1], heptane-2-carboxylic acid, 1-O-[2r-hydroxy-hexadecyl]-sn-glycerol, and1-pentadecene) was developed to identify S-OSA (AUC: 1.000) and distinguishsevere cases from nonsevere cases (AUC: 0.813). An independent cohortwas used to validate the model by distinguishing S-OSA samples fromM-OSA (AUC: 0.729) and CON (AUC: 0.990) samples. Glycolysis pathwayactivation was identified as a characteristic of OSA; it may contributeto diabetes mellitus onset in OSA patients. Dyslipidaemia, foamy macrophageformation, platelet activation, and actin cytoskeleton might collectivelyplay a key role in vascular damage in OSA patients, contributing tothe development of atherosclerosis. These findings reveal molecularbases for OSA-related cardiometabolic complications and provide newdiagnostic biomarkers for OSA and the identification of severe cases.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":"25 9","pages":"4419–4434"},"PeriodicalIF":3.8,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148890349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual Functionality in Whole Blood Microsampling: Combining Plasma Separation and Immunoaffinity Cleanup for LC–MS Based Targeted Protein Analysis 全血显微取样的双重功能：结合血浆分离和免疫亲和清除，用于基于LC-MS的靶向蛋白分析

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2026-09-04 Epub Date: 2026-08-03 DOI: 10.1021/acs.jproteome.6c00092

Ago Mrsa, Bjarke Strøm Larsen, Trine Grønhaug Halvorsen, Léon Reubsaet

{"title":"Dual Functionality in Whole Blood Microsampling: Combining Plasma Separation and Immunoaffinity Cleanup for LC–MS Based Targeted Protein Analysis","authors":"Ago Mrsa, Bjarke Strøm Larsen, Trine Grønhaug Halvorsen, Léon Reubsaet","doi":"10.1021/acs.jproteome.6c00092","DOIUrl":"https://doi.org/10.1021/acs.jproteome.6c00092","url":null,"abstract":"Smart sampling isa concept that combines a traditional microsampling,such as dried blood spot (DBS), with sample preparation initiatedat the moment of sampling. In this paper, plasma separation and antibody-basedaffinity cleanup are integrated into a single 3D-printed samplingdevice. Our goal was to evaluate and validate the analytical performanceof this device for LC-MS-based determination of hCG. The device effectivelyeliminated red blood cells, significantly reducing hemoglobin interferenceby more than 93%. Plasma extraction of >80% was achieved from only20 μL of whole blood. The partial validation in line with theEuropean Medicines Agency’s ICH guidelines showed that theanalytical performance of the sampling device was not compromised.Linear correlation coefficients ≥0.991 were achieved (range:0.5–75 ng/mL). High between-run accuracy for all QC levelswere achieved, ranging from 90 to 105% with a corresponding precisionof ≤11%. In conclusion, the 3D-printed device offers a robustsolution to traditional microsampling limitations, potentially andin the future enabling point-of-care diagnostics with the versatilityand ease of established DBS methods. The successful integration ofplasma separation and smart sampling enhances analytical sensitivityand specificity, representing a promising tool for advancements inclinical bioanalysis.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":"25 9","pages":"4665–4675"},"PeriodicalIF":3.8,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148890297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acidic Stress Induces Proteomic Reprogramming and Virulence-Associated Adaptation in Paracoccidioides brasiliensis 酸性胁迫诱导巴西副球虫蛋白质组重编程和毒力相关适应

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2026-09-04 Epub Date: 2026-08-07 DOI: 10.1021/acs.jproteome.6c00370

Reinaldo Souza Oliveira, Maria Cecilia Gordijo, Wilson Dias Segura, Pedro Henrique Corteletti Manfio, Leonardo Brogliato de Moraes, Sarah Fernandes Lima, Solange M. T. Serrano, Alison Felipe Alencar Chaves, Wagner Luiz Batista

{"title":"Acidic Stress Induces Proteomic Reprogramming and Virulence-Associated Adaptation in Paracoccidioides brasiliensis","authors":"Reinaldo Souza Oliveira, Maria Cecilia Gordijo, Wilson Dias Segura, Pedro Henrique Corteletti Manfio, Leonardo Brogliato de Moraes, Sarah Fernandes Lima, Solange M. T. Serrano, Alison Felipe Alencar Chaves, Wagner Luiz Batista","doi":"10.1021/acs.jproteome.6c00370","DOIUrl":"https://doi.org/10.1021/acs.jproteome.6c00370","url":null,"abstract":"Paracoccidioidomycosis (PCM) is a neglected systemicmycosis whoseetiologic agents must adapt to acidic host niches such as phagolysosomes.Here, we used quantitative liquid chromatography-tandem mass spectrometry(LC-MS/MS)-based proteomics to define the response of Paracoccidioides brasiliensis to acidic stress (pH4.5) versus control pH (6.5) after 5 and 24 h. We identified and quantified4374 proteins, including 327 and 722 differentially abundant proteinsat 5 and 24 h, respectively, revealing time-dependent proteomic reprogramming.Enrichment analyses highlighted proteolysis, protein metabolism, organonitrogenmetabolism, MAPK- and SNF1-like signaling, central carbon metabolism,tyrosine metabolism, and ergosterol biosynthesis as major acid-responsiveprocesses. Complementary assays showed pH-dependent extracellularproteolytic activity, increased adhesion to A549 pulmonary epithelialcells, and dynamic ergosterol remodeling. The proteomic data furtherindicated increased abundance of moonlighting proteins linked to adhesionand metabolic enzymes associated with ATP generation and melanin precursorproduction. Together, these findings indicate that P. brasiliensis adapts to acidic environments throughcoordinated regulation of proteostasis, metabolism, signaling, host-cellinteraction, and membrane homeostasis, supporting survival and virulencepotential in acidic host microenvironments.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":"25 9","pages":"4796–4810"},"PeriodicalIF":3.8,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148890302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Upregulation of Endogenous Serine Proteinases by SVMPs Contributes to Muscle Damage Induced by Bothrops atrox Venom SVMPs上调内源性丝氨酸蛋白酶参与肉毒蛇毒诱导的肌肉损伤

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2026-09-04 Epub Date: 2026-08-25 DOI: 10.1021/acs.jproteome.6c00380

Alison Felipe Alencar Chaves, Emilly Campos Tioyama, Mônica Colombini, Sarah N. C. Gimenes, Isabella Mitie de Camargo, Karina Maria Pereira Silva, Giovanni Perez Machado da Silveira, Savio Stefanini Sant’Anna, Eliana Lima Faquim-Mauro, Solange M. T. Serrano, Ana M. Moura-da-Silva, Luciana A. Freitas-de-Sousa

{"title":"Upregulation of Endogenous Serine Proteinases by SVMPs Contributes to Muscle Damage Induced by Bothrops atrox Venom","authors":"Alison Felipe Alencar Chaves, Emilly Campos Tioyama, Mônica Colombini, Sarah N. C. Gimenes, Isabella Mitie de Camargo, Karina Maria Pereira Silva, Giovanni Perez Machado da Silveira, Savio Stefanini Sant’Anna, Eliana Lima Faquim-Mauro, Solange M. T. Serrano, Ana M. Moura-da-Silva, Luciana A. Freitas-de-Sousa","doi":"10.1021/acs.jproteome.6c00380","DOIUrl":"https://doi.org/10.1021/acs.jproteome.6c00380","url":null,"abstract":"In Brazil, approximately 25,000 snakebites occur annually,with Bothrops atrox responsible formost cases. Localmorbidity is high, driven primarily by snake venom metalloproteases(SVMPs). The major SVMPs in B. atrox venom, Atroxlysin-Ia (ATXL) and Batroxrhagin (BATX), efficientlyhydrolyze extracellular matrix proteins, inducing rapid hemorrhageand dermonecrosis. Thus, we characterized the composition of the exudateproduced after SVMPs injection into the mice gastrocnemius muscleusing proteomics. Muscle damage was evaluated by histological analysis.The composition of the exudate was analyzed by mass spectrometry.The SVMPs induced disorganization of muscle fibers and inflammatorycell migration. However, ATXL-induced a significantly higher neutrophilinflux compared to BATX, likely triggered by an increase in CXCL16,suggesting a superior inflammatory capacity. In summary, despite beingmetalloproteases, these toxins exhibit distinct pathological profiles:ATXL is predominantly inflammatory, while BATX is more hemorrhagic.Interestingly, while endogenous serine proteinase levels were similarin both exudates, BATX showed significantly higher levels of proteinaseinhibitors. Furthermore, identification of peptide bond cleavage sitesrevealed a pattern consistent with trypsin-like serine proteinases.These findings suggest that SVMPs not only damage tissue directlybut also associate with the activation of host endogenous proteinases,which may contribute to the complex pathology of B. atrox envenomation, although direct causation remainsto be established.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":"25 9","pages":"4811–4822"},"PeriodicalIF":3.8,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148890326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Hepatic Lipidome Analysis in Aging Mice 衰老小鼠肝脂质组综合分析

IF 3.8 2区生物学

Journal of Proteome Research Pub Date : 2026-09-04 Epub Date: 2026-08-20 DOI: 10.1021/acs.jproteome.6c00254

Punyatoya Panda, Christina R. Ferreira, Allison J. Schaser, Uma K. Aryal

{"title":"Integrated Hepatic Lipidome Analysis in Aging Mice","authors":"Punyatoya Panda, Christina R. Ferreira, Allison J. Schaser, Uma K. Aryal","doi":"10.1021/acs.jproteome.6c00254","DOIUrl":"https://doi.org/10.1021/acs.jproteome.6c00254","url":null,"abstract":"The liver is a metabolically active organ vital for carbohydrate,protein, and lipid metabolism as well as detoxification of harmfulsubstances. Here, we integrated multiple reaction monitoring (MRM)profiling, untargeted lipidomics, and desorption electrospray ionization-massspectrometry imaging (DESI-MSI) to identify age-associated changesin lipid profiles in the liver of adult mice across three age groups:adult (3–4 months), midaged (10 months), and old (19–21months). Comparative lipidomic analysis revealed age-dependent remodelingof membrane phospholipids and sphingolipids. Aging was associatedwith significant alterations of phosphatidylcholines (PC), lysophosphatidylcholines (LPC), phosphatidylserines (PS), phosphatidylethanolamines (PE),and phosphatidylinositols (PI), characterized by reduced levels ofunsaturated PC species (PC 36:5, PC 36:4) and saturated PC 32:0 alongsideincreased LPCs and highly unsaturated PC 40:7. In parallel, sphingolipidswere also altered, with increased ceramides (Cer 34:1; O2, CerP 36:1;O2, ACer 59:1; O2) and sphingomyelins (SM 34:1; O2, SM 34:0; O2),and decreased levels of longer-chain sphingomyelins (SM 40:1; O2,SM 42:1; O2, SM 40:2; O2) compared with adult and midaged mice. Additionally,DESI-MSI revealed age-associated changes in monounsaturated (palmitoleic,oleic, eicosenoic) and polyunsaturated (linoleic, docosahexaenoic)fatty acids. These lipid alterations were consistently observed acrossmultiple mass spectrometry platforms, including spatially resolvedlipid distributions obtained by DESI-MSI, and are linked to membranestructure, signaling, and metabolism, providing new insights intoage-related liver dysfunction.","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":"25 9","pages":"4782–4795"},"PeriodicalIF":3.8,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148890338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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