New Phytologist最新文献_第8页

The complex interaction between mammalian herbivores, climate, soil and fire has shaped the evolution and distribution of plant spinescence across biogeographical realms. 哺乳动物、草食动物、气候、土壤和火之间复杂的相互作用塑造了植物棘在生物地理领域的进化和分布。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-09-01 Epub Date: 2026-07-22 DOI: 10.1111/nph.71376

Rachel Souza Ferreira, Jens J Ringelberg, Colin Hughes, Eduardo Arlé, Friederike J R Wölke, Kyle W Tomlinson, Renske E Onstein

{"title":"The complex interaction between mammalian herbivores, climate, soil and fire has shaped the evolution and distribution of plant spinescence across biogeographical realms.","authors":"Rachel Souza Ferreira, Jens J Ringelberg, Colin Hughes, Eduardo Arlé, Friederike J R Wölke, Kyle W Tomlinson, Renske E Onstein","doi":"10.1111/nph.71376","DOIUrl":"10.1111/nph.71376","url":null,"abstract":"The evolutionary arms race between plants and herbivores led to numerous plant adaptations, including spinescence. However, the balance between herbivory and abiotic conditions in the evolution of spinescence remains unclear. We integrated phylogenetic, geographic, and trait data for 2686 species of an ecologically diverse and spinescent pantropical lineage, mimosoid legumes, with distribution data on 368 extant and extinct mammalian herbivores ≥ 10 kg. Using structural equation models, we assessed how herbivores, climate, soil, and fire directly and indirectly affected the proportion of spinescent mimosoids across global and continental assemblages. Models incorporating extinct herbivore assemblages explained more variation in the proportion of spiny species than those based solely on extant herbivores. Dry-season length and soil pH increased spinescence both directly and indirectly through their effects on herbivore richness. These abiotic effects exceeded herbivore effects at continental scales. Fire influenced spinescence only indirectly via its positive relationship with herbivore richness. Finally, spinescence evolved repeatedly across mimosoids from c. 35 million years ago, pre-dating the Miocene savanna expansion. Our study suggests that past herbivore communities have left a lasting imprint on present-day plant defence patterns and that long-term climatic transitions and the emergence of open, herbivore-rich landscapes played crucial roles in the evolution and distribution of spinescence.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"3640-3655"},"PeriodicalIF":8.1,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13491257/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148551184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A C₂H₂ zinc finger transcription factor links a GWAS locus to linear growth in Gracilariopsis lemaneiformis via MCM helicase activation. C2H2锌指转录因子通过MCM解旋酶激活将GWAS位点与薄叶冈的线性生长联系起来。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-09-01 Epub Date: 2026-05-31 DOI: 10.1111/nph.71281

Youtao Huang, Xinran Wang, Mengxing Cao, Zhenghong Sui

{"title":"A C2H2 zinc finger transcription factor links a GWAS locus to linear growth in Gracilariopsis lemaneiformis via MCM helicase activation.","authors":"Youtao Huang, Xinran Wang, Mengxing Cao, Zhenghong Sui","doi":"10.1111/nph.71281","DOIUrl":"10.1111/nph.71281","url":null,"abstract":"The genetic basis of linear growth rate in the red alga Gracilariopsis lemaneiformis remains poorly characterized. This study functionally characterized the gene LXC001294, a prior genome-wide association studies (GWAS) candidate associated with growth variation, to elucidate its molecular role. We employed phylogenetic analysis, yeast assays, and subcellular localization to characterize LXC001294. Its genome-wide binding sites were identified by ChIP-seq, and transcriptomic profiles of fast- (YT13) and slow-growing (QHD9) strains were compared via RNA-seq. Direct regulation of key targets was validated using yeast one-hybrid and dual-luciferase assays. LXC001294 was confirmed as a nuclear-localized C2H2 zinc finger transcription factor. Integrated multi-omics analysis revealed its direct targeting and upregulation of five minichromosome maintenance (MCM) genes, core components of the DNA replication machinery, in the fast-growing strain. We demonstrate that LXC001294 promotes algal growth by activating the MCM complex, thereby enhancing DNA replication. This study provides a mechanistic link from a GWAS signal to a growth phenotype in a macroalga and establishes the transcriptional regulation of replication licensing as a key node controlling growth rate in nonmodel species.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"2558-2573"},"PeriodicalIF":8.1,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13442993/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148139539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Climate-induced shifts in ectomycorrhizal explorations from long to short strategies along an elevation gradient. 气候诱导的外生菌根探索沿海拔梯度从长策略到短策略的转变。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-09-01 Epub Date: 2026-05-13 DOI: 10.1111/nph.71237

Lulu Chen, Richard D Bardgett, Wenjing Zeng, Lijuan Sun, Zeqing Ma

{"title":"Climate-induced shifts in ectomycorrhizal explorations from long to short strategies along an elevation gradient.","authors":"Lulu Chen, Richard D Bardgett, Wenjing Zeng, Lijuan Sun, Zeqing Ma","doi":"10.1111/nph.71237","DOIUrl":"10.1111/nph.71237","url":null,"abstract":"Ectomycorrhizal (EcM) fungi colonize over 60% of tree stems globally, yet how their hyphal morphology varies across environments remains unclear, and current methods for defining EcM mycelial morphology and foraging space lack reliability. Here, we analyzed the morphological traits of 53 262 EcM root tips of Abies fargesii var. faxoniana across elevational temperature gradients (0.2-5.1°C) and developed a species-morphotype-space (SMS) framework to quantify mycelial foraging strategies from root tips to the soil profile. Our results revealed considerable EcM morphotype complexity at the root tip level and distinct temperature- and soil nutrient-driven shifts in EcM morphology based on an elevational gradient. Short-hyphae dominated at lower elevation, whereas thicker long-rhizomorphs prevailed at higher elevation, reaching a foraging space of up to 1.02 × 106 cm3 m-3 soil. Short-hyphae mycorrhizas exhibited highly active hydrolytic enzymes, which enable them to increase resource availability and play a pivotal role in nutrient foraging. As temperatures rise, increased soil inorganic nitrogen availability in cold forests favors short-hyphae while suppressing long-rhizomorphs with high carbon retention capacity, potentially reducing soil carbon stability. Our analyses of EcM foraging space and multidimensional mycelial diversity provide new opportunities to predict how EcM hyphal processes respond to climate change.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"3558-3575"},"PeriodicalIF":8.1,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13491293/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147934712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arbuscular mycorrhiza provides postanthesis benefits to maximize wheat grain yield and nitrogen concentration. 丛枝菌根对提高小麦籽粒产量和氮浓度具有后效作用。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-09-01 Epub Date: 2026-06-18 DOI: 10.1111/nph.71353

Jie Zhou, Baowei Huang, Ruoyan Yang, Fede Berckx, Heng Gui, Petra Fransson, Matthias C Rillig, Matthew Reynolds, Junjie Xie, Bahar S Razavi, Feng-Min Li, Xin Chen, Martin Weih, Haishui Yang

{"title":"Arbuscular mycorrhiza provides postanthesis benefits to maximize wheat grain yield and nitrogen concentration.","authors":"Jie Zhou, Baowei Huang, Ruoyan Yang, Fede Berckx, Heng Gui, Petra Fransson, Matthias C Rillig, Matthew Reynolds, Junjie Xie, Bahar S Razavi, Feng-Min Li, Xin Chen, Martin Weih, Haishui Yang","doi":"10.1111/nph.71353","DOIUrl":"10.1111/nph.71353","url":null,"abstract":"A trade-off between high grain yield and high protein (or nitrogen, N) concentration is frequently observed for crop plants in agroecosystems and is difficult to resolve using conventional agricultural methods. Whether ecological strategies, such as exploring the interactions between plants and microbes, can be leveraged to tackle this problem remains unclear. Here, we used wheat as a model plant and focused on the interaction among plants, arbuscular mycorrhizal fungi (AMF) and hyphosphere bacteria. We conducted six independent but complementary experiments and demonstrated that AMF can enhance both wheat yield and grain N concentration, but only in specific varieties with high N conversion efficiency (grain yield per mean plant N at preanthesis). AMF can boost yield from increased grain weight via enhanced postanthesis photosynthetic carbon (C) assimilation through promoting plant phosphorus uptake, while elevating grain N concentration through stimulating postanthesis N uptake and assimilation. This dual effect arises from the enhanced postanthesis belowground C allocation, sustaining AMF hyphal network integrity and increasing soil nutrient availability via reshaping hyphosphere bacterial community structure. Our findings indicate that postanthesis plant-microbial interactions can potentially overcome an old dilemma between maximizing grain yield and N concentration in agricultural production.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"3576-3591"},"PeriodicalIF":8.1,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13491297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148279339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated multi-omics decipher the complex nodule microbiota and distinct Frankiaceae symbiotic traits in wild actinorhizal plants. 综合多组学分析了野生放线根植物中复杂的根瘤菌群和独特的Frankiaceae共生性状。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-09-01 Epub Date: 2026-05-08 DOI: 10.1111/nph.71234

Xinyue Luo, Zhengfu Lei, Dongming Fang, Hongyun Chen, Lishen Qian, Canzhi Jin, Xiaolin Wang, Xin Liu, Huan Liu, Yayu Wang

{"title":"Integrated multi-omics decipher the complex nodule microbiota and distinct Frankiaceae symbiotic traits in wild actinorhizal plants.","authors":"Xinyue Luo, Zhengfu Lei, Dongming Fang, Hongyun Chen, Lishen Qian, Canzhi Jin, Xiaolin Wang, Xin Liu, Huan Liu, Yayu Wang","doi":"10.1111/nph.71234","DOIUrl":"10.1111/nph.71234","url":null,"abstract":"Actinorhizal plants are ecologically important pioneer species in temperate regions, capable of nitrogen-fixing root nodule symbiosis with Frankiaceae bacteria. Despite their significance within the nitrogen-fixing clades (NFC), multi-omics studies of actinorhizal symbiosis remain scarce. We profiled prokaryotic communities in the rhizosphere, root, and/or nodule compartments from five phylogenetically representative actinorhizal species, three legumes, and four nonnodulated NFC species using 16S rDNA sequencing. Transcriptomic and metagenomic analyses were performed on actinorhizal roots and nodules, respectively. Metagenome-assembled genomes revealed four novel Frankiaceae species. Frankiae relative abundance levels in nodules were generally lower than rhizobia in legumes. Actinorhizal nodules harbour diverse bacterial taxa, which exhibit predominantly positive interactions, with Frankiae forming a tightly interacting subgroup. Actinorhizal plants engage actively with soil microbiota, recruiting a specific rhizosphere community enriched with beneficial microbes, including ammonia-oxidising archaea. Many symbiotic mechanisms in nodulating host plants are conserved and derived from pre-existing molecular modules. Our analysis suggests the phosphoinositide signalling likely functions in actinorhizal symbiotic signal transduction. However, Frankiae exhibit fundamentally different symbiotic functional characteristics compared to rhizobia, reflecting less intimate symbiosis, which might favour the life-history strategies of temperate perennial actinorhizal plants.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"2832-2851"},"PeriodicalIF":8.1,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13442980/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147857490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A cyclo-DOPA 6-O-glucosyltransferase-mediated route for gomphrenin I biosynthesis in Basella alba and Gomphrena globosa. 环- dopa 6- o-葡萄糖基转移酶介导的白底螺和球孔螺中gomphrenin I生物合成途径

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-09-01 Epub Date: 2026-06-07 DOI: 10.1111/nph.71340

Tomohiro Imamura, Ryouta Shigehisa, Akio Miyazato, Nami Matsumura, Kaisei Miyaki, Tenta Segawa, Masahide Yoshizumi, Hiroki Takagi, Takumi Yamaguchi, Shinya Ohki, Masashi Mori

{"title":"A cyclo-DOPA 6-O-glucosyltransferase-mediated route for gomphrenin I biosynthesis in Basella alba and Gomphrena globosa.","authors":"Tomohiro Imamura, Ryouta Shigehisa, Akio Miyazato, Nami Matsumura, Kaisei Miyaki, Tenta Segawa, Masahide Yoshizumi, Hiroki Takagi, Takumi Yamaguchi, Shinya Ohki, Masashi Mori","doi":"10.1111/nph.71340","DOIUrl":"10.1111/nph.71340","url":null,"abstract":"Betacyanins are red pigments characteristic of Caryophyllales and show considerable structural diversity, yet the enzymatic basis underlying 6-O-glucosylated betacyanins, such as gomphrenin I, has remained unclear. In particular, how alternative glucosylation patterns contribute to betacyanin diversification is poorly understood. Here, we identified cyclo-DOPA glucosyltransferases from Basella alba and Gomphrena globosa and examined their roles in gomphrenin I biosynthesis using transient expression assays and tobacco BY-2 cell systems. Phylogenetic analyses, structural modelling, and site-directed mutagenesis were employed to investigate their functional and structural characteristics. BacDOPA5/6GTs catalysed both 5-O- and 6-O-glucosylation of cyclo-DOPA, leading to the production of betanin and gomphrenin I, whereas GgcDOPA6GT specifically mediated gomphrenin I formation. These enzymes belong to distinct subclades within the cDOPA-GT family, and mutational analyses demonstrated essential roles for conserved histidine residues and an α-helical region adjacent to the catalytic site. Thermal stability analyses further showed that gomphrenin I is more thermally stable than betanin, likely due to the formation of an intramolecular hydrogen bond. Together, these results reveal an additional cDOPA6GT-mediated route for gomphrenin I biosynthesis and provide insight into the diversification and functional specialisation of betacyanins, linking the position of glucosylation to pigment stability and biochemical properties.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"2706-2724"},"PeriodicalIF":8.1,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13443012/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148200568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trp521 oxidation affects FtsH2 stability and its role in PSII repair. Trp521氧化影响FtsH2的稳定性及其在PSII修复中的作用。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-09-01 Epub Date: 2026-06-14 DOI: 10.1111/nph.71356

Jingzhi Zhang, Keun Pyo Lee, Yujie Lou, Yanling Liu, Qihua Ling, Chanhong Kim

{"title":"Trp521 oxidation affects FtsH2 stability and its role in PSII repair.","authors":"Jingzhi Zhang, Keun Pyo Lee, Yujie Lou, Yanling Liu, Qihua Ling, Chanhong Kim","doi":"10.1111/nph.71356","DOIUrl":"10.1111/nph.71356","url":null,"abstract":"Photosynthesis generates reactive oxygen species that damage photosynthetic machinery. Singlet oxygen (1O2) produced at photosystem II (PSII) damages PSII proteins, with D1 as a primary target due to its proximity to the site of 1O2 generation. During PSII repair, D1 is degraded by the FtsH metalloprotease complex, followed by resynthesis and PSII reassembly. Oxidation of a conserved tryptophan residue (Trp14) in N-terminal D1 enhances its recognition and degradation by FtsH, serving as a regulatory signal under photooxidative stress. The FtsH complex also undergoes turnover, but the mechanism remains unclear. In Arabidopsis thaliana, the FtsH complex comprises four isomers (FtsH1/2/5/8), grouped into types A and B. We identified a conserved, oxidation-prone Trp residue in type B isomers (FtsH2/8). Using transgenic plants expressing FtsH2 variants with Trp substitutions, we found that Trp oxidation affects FtsH2 stability and its ability to degrade D1. In ftsh2 ftsh8 double mutants, expressing FtsH2 with a Trp-to-phenylalanine substitution (oxidation-mimicking) failed to sustain growth and did not survive to reproduction, whereas a Trp-to-leucine substitution (oxidation-insensitive) enhanced PSII repair and improved growth under conditions tested. These findings demonstrate that oxidative modification influences FtsH2 function and provide mechanistic insight into how redox-sensitive residues affect PSII repair and chloroplast protein homeostasis.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"2588-2603"},"PeriodicalIF":8.1,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13443278/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148254097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The third dimension of root economics space. 根经济学空间的第三维度。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-08-28 DOI: 10.1111/nph.71545

Elise Gallois, Lisa Pilkinton

引用次数: 0

Low-temperature range limits of trees explained by freezing tolerance, current microclimate and historical extremes. 低温范围的限制解释了树木的耐寒性，当前的小气候和历史极端。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-08-16 DOI: 10.1111/nph.71504

Yang Yang, Zhi Chao Wu, Yue Qi, Shao Yu Gao, Hang Sun, Christian Körner

{"title":"Low-temperature range limits of trees explained by freezing tolerance, current microclimate and historical extremes.","authors":"Yang Yang, Zhi Chao Wu, Yue Qi, Shao Yu Gao, Hang Sun, Christian Körner","doi":"10.1111/nph.71504","DOIUrl":"10.1111/nph.71504","url":null,"abstract":"Explaining the low-temperature limit of trees requires in situ information on the actual temperature conditions, the trees' phenology and freezing tolerance. Here, we report the seasonal course of freezing resistance and its response to local temperature in species of Larix and Abies, forming the treeline (4350-4400 m) in the south-eastern Himalayas, and Quercus and tree-size Rhododendron, reaching their upper limit in the immediate neighborhood (4270-4290 m). By linking tree-crown temperatures with on-site air temperature, we can utilize long-term meteorological records to hindcast critical freezing events. Deciduous Larix flushes earliest and exhibits the greatest freezing tolerance and largest safety margin. The evergreen species flush 2 months later and operate near their freezing damage limit. The conifers can compensate for freezing damage by rapid resprouting from small auxiliary buds, not an option for Quercus and Rhododendron with their bigger buds. Freezing damage does not appear to constrain the two treeline conifers. Their range limit most likely reflects the general low-temperature growth limit of the tree life form. Freezing tolerance is most likely controlling the upper limits of the evergreen broad-leaved species. The results offer a mechanistic explanation of the range limit of these high-elevation tree species.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":8.1,"publicationDate":"2026-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148765667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

microRNA-mediated control of cell fate specification and patterning in Marchantia polymorpha. 微rna介导的多形地药细胞命运规范和模式的调控。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-08-09 DOI: 10.1111/nph.71487

Adolfo Aguilar-Cruz, Eduardo Flores-Sandoval, Ye Xu, Yu Yu, Ximena Gutiérrez-Ramos, Cristian Y González-Rangel, Omar Oltehua-López, Ana E Dorantes-Acosta, Joshua T Trujillo, Hirotaka Kato, Kimitsune Ishizaki, Rebecca A Mosher, Liam Dolan, Xuemei Chen, Daniel Grimanelli, Jim Haseloff, John L Bowman, Mario A Arteaga-Vazquez

{"title":"microRNA-mediated control of cell fate specification and patterning in Marchantia polymorpha.","authors":"Adolfo Aguilar-Cruz, Eduardo Flores-Sandoval, Ye Xu, Yu Yu, Ximena Gutiérrez-Ramos, Cristian Y González-Rangel, Omar Oltehua-López, Ana E Dorantes-Acosta, Joshua T Trujillo, Hirotaka Kato, Kimitsune Ishizaki, Rebecca A Mosher, Liam Dolan, Xuemei Chen, Daniel Grimanelli, Jim Haseloff, John L Bowman, Mario A Arteaga-Vazquez","doi":"10.1111/nph.71487","DOIUrl":"https://doi.org/10.1111/nph.71487","url":null,"abstract":"MicroRNAs (miRNAs) are small non-coding RNA molecules essential for growth and development in eukaryotes. In plants, the master gene DICER-LIKE 1 (DCL1) catalyzes the biogenesis of miRNAs by processing double-stranded precursors that give rise to mature miRNAs. We sought to understand the function and evolution of microRNAs using Marchantia polymorpha, a model bryophyte that allows comparative approaches to infer characteristics of the ancestral land plant. We functionally characterized loss-of-function mutants of MpDCL1 a generated by means of CRISPR-Cas9-mediated genomic edition and the miR166/CLASS III HD-ZIP regulatory circuit in Marchantia polymorpha. We report that MpDCL1a is required for the biogenesis of miRNAs and uncovered a central role for miR166/Homeodomain Zipper Class III-regulated auxin synthesis in the specification of cell identity, patterning, meristem function, laminar expansion, and the development of the body in the last common ancestor of the bryophytes and vascular plants. Our findings indicate that DCL1, Class III HD-ZIP, miR166, and auxin functioned in the development of the body of the last common ancestor of extant land plants and provide a novel working framework to interrogate the basic principles of cell specification and patterning in plants.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":8.1,"publicationDate":"2026-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148702259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0