The Arabidopsis eIF4E1 regulates NRT1.1-mediated nitrate signaling at both translational and transcriptional levels

IF 9.4 1区生物学 Q1 Agricultural and Biological Sciences

New Phytologist Pub Date : 2023-07-10 DOI:10.1111/nph.19129

Na Li, Yawen Duan, Qing Ye, Yuhan Ma, Rongjie Ma, Lufei Zhao, Sirui Zhu, Feng Yu, Shengdong Qi, Yong Wang

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引用次数: 0

Abstract

Identifying new nitrate regulatory genes and illustrating their mechanisms in modulating nitrate signaling are of great significance for achieving the high yield and nitrogen use efficiency (NUE) of crops.
Here, we screened a mutant with defects in nitrate response and mapped the mutation to the gene eIF4E1 in Arabidopsis. Our results showed that eIF4E1 regulated nitrate signaling and metabolism. Ribo-seq and polysome profiling analysis revealed that eIF4E1 modulated the amount of some nitrogen (N)-related mRNAs being translated, especially the mRNA of NRT1.1 was reduced in the eif4e1 mutant.
RNA-Seq results enriched some N-related genes, supporting that eIF4E1 is involved in nitrate regulation. The genetic analysis indicated that eIF4E1 worked upstream of NRT1.1 in nitrate signaling. In addition, an eIF4E1-interacting protein GEMIN2 was identified and found to be involved in nitrate signaling. Further investigation showed that overexpression of eIF4E1 promoted plant growth and enhanced yield and NUE.
These results demonstrate that eIF4E1 regulates nitrate signaling by modulating NRT1.1 at both translational and transcriptional levels, laying the foundation for future research on the regulation of mineral nutrition at the translational level.

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拟南芥eIF4E1在翻译和转录水平上调控nrt1.1介导的硝酸盐信号

发现新的硝酸盐调控基因并阐明其调控硝酸盐信号的机制，对实现作物高产和氮素利用效率具有重要意义。在这里，我们筛选了一个硝酸盐响应缺陷的突变体，并将该突变定位到拟南芥的eIF4E1基因上。我们的研究结果表明，eIF4E1调节硝酸盐信号传导和代谢。Ribo-seq和多体分析显示，eIF4E1突变体调节了一些氮(N)相关mRNA的翻译量，特别是NRT1.1 mRNA的翻译量减少。RNA-Seq结果富集了部分n相关基因，支持eIF4E1参与硝酸盐调控。遗传分析表明，eIF4E1在硝酸盐信号传导中位于NRT1.1的上游。此外，还发现了一个与eif4e1相互作用的蛋白GEMIN2参与硝酸盐信号传导。进一步研究表明，过表达eIF4E1可促进植株生长，提高产量和氮肥利用率。这些结果表明，eIF4E1在翻译和转录水平上通过调控NRT1.1调控硝酸盐信号，为今后在翻译水平上研究矿质营养调控奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

New Phytologist PLANT SCIENCES-

CiteScore

17.60

自引率

5.30%

发文量

728

审稿时长

1 months

期刊介绍： New Phytologist is a leading publication that showcases exceptional and groundbreaking research in plant science and its practical applications. With a focus on five distinct sections - Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology - the journal covers a wide array of topics ranging from cellular processes to the impact of global environmental changes. We encourage the use of interdisciplinary approaches, and our content is structured to reflect this. Our journal acknowledges the diverse techniques employed in plant science, including molecular and cell biology, functional genomics, modeling, and system-based approaches, across various subfields.