New Phytologist最新文献

Climate adaptation in Populus trichocarpa: key adaptive loci identified for stomata and leaf traits. 毛杨的气候适应：气孔和叶片性状的关键适应位点。

IF 8.1 1区生物学

New Phytologist Pub Date : 2025-07-29 DOI: 10.1111/nph.70343

Marie C Klein, Zi Meng, Jack Bailey-Bale, Suzanne Milner, Peicai Shi, Wellington Muchero, Jin-Gui Chen, Timothy J Tschaplinski, Daniel Jacobson, John Lagergren, Matthew Lane, Chris O'Brien, Hari Chhetri, Chanaka Roshan Abeyratne, Mengjun Shu, Peter Freer-Smith, Thomas N Buckley, Troy S Magney, J Grey Monroe, Gerald A Tuskan, Gail Taylor

{"title":"Climate adaptation in Populus trichocarpa: key adaptive loci identified for stomata and leaf traits.","authors":"Marie C Klein, Zi Meng, Jack Bailey-Bale, Suzanne Milner, Peicai Shi, Wellington Muchero, Jin-Gui Chen, Timothy J Tschaplinski, Daniel Jacobson, John Lagergren, Matthew Lane, Chris O'Brien, Hari Chhetri, Chanaka Roshan Abeyratne, Mengjun Shu, Peter Freer-Smith, Thomas N Buckley, Troy S Magney, J Grey Monroe, Gerald A Tuskan, Gail Taylor","doi":"10.1111/nph.70343","DOIUrl":"https://doi.org/10.1111/nph.70343","url":null,"abstract":"We investigated adaptive genetic variation in Populus trichocarpa, a potential biofuel feedstock crop, to better understand how physiological traits may influence tolerance to water limitation. Our study focused on leaf and stomatal traits, given their roles in plant-water relations and adaptation. Using a diversity panel of over 1300 genotypes, we measured 14 leaf and stomatal traits under control (well-watered) and drought (water-limited) conditions. We conducted genome-wide association studies (GWAS), climate association analyses, and transcriptome (RNA-seq) profiling to identify genetic loci associated with phenotypic variation and adaptation. Stomatal traits, including size and density, were correlated with the climate of origin, with genotypes from more arid regions tending to have smaller but denser stomata. GWAS identified multiple loci associated with trait variation, including a major-effect region on chromosome 10 linked to stomatal size and abaxial contact angle. This locus overlapped with a tandem array of 3-ketoacyl-CoA synthase (KCS) genes and showed strong allele-climate and gene expression associations. Our findings reveal genetic and phenotypic variation consistent with local adaptation and suggest that future climates may favor alleles associated with smaller stomata, particularly under increasing aridity. This work provides insights into climate adaptation and breeding strategies for resilience in perennial crops.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":8.1,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144734506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ustilaginoidea virens suppresses floral immunity through promoting GA biosynthesis by the effector SCRE9. 紫花菇通过促进GA的合成抑制花免疫。

IF 8.1 1区生物学

New Phytologist Pub Date : 2025-07-27 DOI: 10.1111/nph.70414

Siwen Yu, Shaoqi Zhang, Xinhang Zheng, Xiaoai Li, Jiyang Wang, Guohua Duan, Shanshan Qiu, Dan Zhao, Nan Nan, Qianheng Yu, Chunquan Jiang, Zhao Peng, Dayong Li, Wenxian Sun

{"title":"Ustilaginoidea virens suppresses floral immunity through promoting GA biosynthesis by the effector SCRE9.","authors":"Siwen Yu, Shaoqi Zhang, Xinhang Zheng, Xiaoai Li, Jiyang Wang, Guohua Duan, Shanshan Qiu, Dan Zhao, Nan Nan, Qianheng Yu, Chunquan Jiang, Zhao Peng, Dayong Li, Wenxian Sun","doi":"10.1111/nph.70414","DOIUrl":"https://doi.org/10.1111/nph.70414","url":null,"abstract":"Rice false smut caused by Ustilaginoidea virens has become one of the most important rice diseases. Ustilaginoidea virens specifically infects rice florets through stamen filaments. However, it is mysterious how U. virens inhibits floral immunity for successful colonization. Here, we show that a small cysteine-rich effector SCRE9 is a key virulence factor in U. virens. Expression of SCRE9 in rice not only suppresses immune responses and false smut resistance but also significantly increases expression of expansin and gibberellin (GA) biosynthetic genes and GA content in young rice panicles. OsSIP1 (SCRE9-interacting protein 1) in rice is targeted by small cysteine-rich effector 9 (SCRE9) and interacts with the OsMADS63 and OsMADS68 transcription factors that transcriptionally regulate the GA synthesis gene GA3ox1. Interestingly, the chloroplast-localized OsSIP1 recruits OsMADS63/68 into the chloroplast. SCRE9 causes OsSIP1 to be translocated into the nucleus, thereby releasing OsMADS63/68 from the chloroplast into the nucleus to promote GA3ox1 transcription. Therefore, SCRE9 promotes GA biosynthesis and expression of expansins in rice florets, thus loosening cell walls and overcoming the physical barrier during U. virens infection. The finding uncovers an unidentified floret infection strategy that offsets the deficiency in cell wall-degrading ability in the unique floret-colonizing fungus.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":8.1,"publicationDate":"2025-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144734507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selection strengthens the relationship between plant diversity and the metabolic profile of Plantago lanceolata. 选择强化了植物多样性与车前草代谢谱之间的关系。

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-07-24 DOI: 10.1111/nph.70340

Pamela Medina-van Berkum, Francesca De Giorgi, Beate Rothe, Walter Durka, Jonathan Gershenzon, Christiane Roscher, Sybille B Unsicker

{"title":"Selection strengthens the relationship between plant diversity and the metabolic profile of Plantago lanceolata.","authors":"Pamela Medina-van Berkum, Francesca De Giorgi, Beate Rothe, Walter Durka, Jonathan Gershenzon, Christiane Roscher, Sybille B Unsicker","doi":"10.1111/nph.70340","DOIUrl":"https://doi.org/10.1111/nph.70340","url":null,"abstract":"Plant species diversity enhances community productivity, but how plant diversity impacts the metabolome of individual plants and the underlying eco-evolutionary processes remains unclear. This study investigated how plant species diversity and selection for growing in different diversity environments affect the leaf metabolome of Plantago lanceolata. We compared the metabolites of plants derived from those that had been selected in the 'Jena Experiment' for 17 yr in plant communities with differing plant diversity with the metabolites of naïve plants not subjected to this selection. The metabolic profiles of selected P. lanceolata plants were also compared after growing in experimental environments varying in plant diversity, soil history and community plant history. Volatile compound diversity in P. lanceolata decreased with plant species richness (SR), primarily due to phenotypic plasticity rather than selection. Soil history further strengthened this relationship. Conversely, non-volatile compound diversity increased with plant SR, but only in phytometers subjected to diversity-driven selection. These effects were more pronounced when plants shared soil-plant history with their community. In summary, our study revealed that both plastic and adaptive responses shape the metabolome of P. lanceolata in relation to plant diversity, with these effects becoming stronger as plant and soil communities mature.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144700146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sarah E. O'Connor. 莎拉·e·奥康纳。

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-07-18 DOI: 10.1111/nph.70394

引用次数: 0

Genomic studies in Linum shed light on the evolution of the distyly supergene and the molecular basis of convergent floral evolution. 通过对亚麻属植物的基因组研究，揭示了亚麻属植物近缘表基因的进化和趋同花进化的分子基础。

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-07-18 DOI: 10.1111/nph.70392

Panagiotis-Ioannis Zervakis, Zoé Postel, Aleksandra Losvik, Marco Fracassetti, Lucile Solér, Estelle Proux-Wéra, Ignas Bunikis, Allison Churcher, Tanja Slotte

{"title":"Genomic studies in Linum shed light on the evolution of the distyly supergene and the molecular basis of convergent floral evolution.","authors":"Panagiotis-Ioannis Zervakis, Zoé Postel, Aleksandra Losvik, Marco Fracassetti, Lucile Solér, Estelle Proux-Wéra, Ignas Bunikis, Allison Churcher, Tanja Slotte","doi":"10.1111/nph.70392","DOIUrl":"https://doi.org/10.1111/nph.70392","url":null,"abstract":"Distyly, an example of convergent evolution, is governed by a supergene, the S-locus, in several species. Recent studies highlight similar genomic architectures of independently evolved S-loci, but its mode of origin and whether similar regulatory pathways underlie the convergent evolution of distyly remains unclear. We examined the evolution of supergenes and mechanisms underlying distyly in Linum species that diverged c. 33 million years ago (Ma). Using haplotype-resolved genomes and population genomics, we identified and characterized the S-loci of Linum perenne (distylous) and Linum grandiflorum (style length dimorphic), and compared them to that of Linum tenue (distylous). We then tested for a conserved hormonal mechanism regulating style length polymorphism in Linum. The S-locus supergene was consistently hemizygous in short-styled individuals across all three species, although it showed variation in size, gene content, repeat elements and extent of recombination suppression. Two S-linked candidate genes, TSS1 (style length) and WDR-44 (anther height/pollen self-incompatibility), were conserved. Consistent with a brassinosteroid-dependent role of TSS1, epibrassinolide treatment revealed a conserved, morph-specific effect on style length. S-locus structural polymorphism, candidate distyly genes and mechanisms regulating style length remain conserved > 30 Ma in Linum. In combination with findings from other systems, our results suggest that the brassinosteroid pathway frequently contributes to style length polymorphism.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144668791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Semiautomatic quantification of 3D Histone H3 phosphorylation signals during cell division in Arabidopsis root meristems. 拟南芥根分生组织细胞分裂过程中组蛋白H3磷酸化信号的半自动定量分析。

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-07-07 DOI: 10.1111/nph.70365

Adrienn Kelemen, Magalie Uyttewaal, Csaba Máthé, Philippe Andrey, David Bouchez, Martine Pastuglia

{"title":"Semiautomatic quantification of 3D Histone H3 phosphorylation signals during cell division in Arabidopsis root meristems.","authors":"Adrienn Kelemen, Magalie Uyttewaal, Csaba Máthé, Philippe Andrey, David Bouchez, Martine Pastuglia","doi":"10.1111/nph.70365","DOIUrl":"https://doi.org/10.1111/nph.70365","url":null,"abstract":"Posttranslational modification of histones during the cell cycle is a major process controlling many aspects of cell division. Among the variety of histone modifications, mitotic phosphorylation of histone H3 at serine 10 (H3S10ph) plays a crucial role, particularly in proper chromosome segregation. Here we aimed at precisely quantifying this phosphorylation dynamics during mitosis in plant cells in order to reveal molecular pathways involved in this process. We describe an analysis pipeline based on 3D image analysis that allows to semiautomatically quantify H3S10ph in mitotic Arabidopsis root cells. We also developed a new method for the compensation of signal attenuation in Z, based on measurement of objects of interest themselves. We show that this new attenuation correction method allows significant gains in accuracy and statistical power. Using this pipeline, we were able to reveal small H3S10ph differences between cells treated with hesperadin, an inhibitor of an H3S10ph kinase, or between Arabidopsis mutants affected in PP2A phosphatase activity. This tool opens new avenues to explore such regulatory pathways in plants, using the wealth of genetic materials available in Arabidopsis. It can also be applied to study other histone posttranslational modifications and more generally to any discrete 3D signals.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144576765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differing metabolic responses of guard cells to blue light. 保卫细胞对蓝光的不同代谢反应。

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-07-04 DOI: 10.1111/nph.70375

Alisdair R Fernie, Stefan Timm

引用次数: 0

OsZNHIT3 functions as a transcriptional activator to regulate tapetal programmed cell death and exine formation in rice. OsZNHIT3作为转录激活因子调控水稻绒毡层程序性细胞死亡和外叶形成。

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-07-04 DOI: 10.1111/nph.70366

Wang Wang, Feng Liu, Haodong Shen, Tiancheng Hao, Xiaoyun Tan, Kun Chen, Tianqi Duan, Jingyi Li, Liyan Bu, Shiqi Song, Nan Wang, Huiting Yang, Yifeng Xu, Huadong Zhan, Yiqun Bao

{"title":"OsZNHIT3 functions as a transcriptional activator to regulate tapetal programmed cell death and exine formation in rice.","authors":"Wang Wang, Feng Liu, Haodong Shen, Tiancheng Hao, Xiaoyun Tan, Kun Chen, Tianqi Duan, Jingyi Li, Liyan Bu, Shiqi Song, Nan Wang, Huiting Yang, Yifeng Xu, Huadong Zhan, Yiqun Bao","doi":"10.1111/nph.70366","DOIUrl":"https://doi.org/10.1111/nph.70366","url":null,"abstract":"Proper timing of programmed cell death (PCD) in the tapetum and the deposition of pollen wall are crucial for gametophyte development. Although several genes involved in these processes have been identified, the regulatory mechanisms remain poorly understood. We isolated and characterized a rice male-sterile mutant, exine abnormal 1 (exa1), which exhibits pale anthers and aborted pollen. The exa1 mutant displays delayed tapetal PCD and defective pollen wall patterning, including thinner sexine and thicker nexine. EXA1 (Exine Abnormal 1), preferentially expressed in the tapetum and microspores, encodes a novel Zf-HIT (Zinc finger-High Temperature) family protein, OsZNHIT3. Nucleus and cytoplasm dual-localized EXA1 functions as a transcriptional activator via its previously undefined C-terminal domain. Phenotypic similarities in rice expressing EXA1-SRDX (SUPERMAN Repressive Domain X) fusion proteins further support its role as a transcriptional activator. PTC1 (Persistent Tapetal Cell 1), EAT1 (Eternal Tapetum 1), and their downstream genes were significantly downregulated in exa1 mutant anthers. Introducing pEXA1:PTC1 or pEXA1:EAT1 into the exa1 mutant partially rescued anther appearance, pollen morphology, and sexine-nexine ratio. This suggests that EXA1 acts genetically upstream of PTC1 and EAT1 in the regulatory pathway. Our study reveals a novel and essential role for EXA1 as a transcriptional activator in rice anther development.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144561606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond conservation: the landscape of chloroplast genome rearrangements in angiosperms. 超越保护：被子植物叶绿体基因组重排的景观。

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-07-04 DOI: 10.1111/nph.70364

Luiz Augusto Cauz-Santos

{"title":"Beyond conservation: the landscape of chloroplast genome rearrangements in angiosperms.","authors":"Luiz Augusto Cauz-Santos","doi":"10.1111/nph.70364","DOIUrl":"https://doi.org/10.1111/nph.70364","url":null,"abstract":"Chloroplast genomes (plastomes) have long been considered structurally conserved, but recent sequencing efforts have uncovered pervasive rearrangements that challenge this assumption. This review catalogues the main types of plastome modifications: large and small inversions; insertions and deletions (indels); gene and intron losses; horizontal gene transfers; shifts in inverted repeat boundaries; and gene duplications. It then explains the molecular processes that generate these changes, from repeat-mediated recombination and slipped-strand mispairing to rare foreign-DNA integration events. These structural variants serve as informative phylogenetic markers, enabling resolution of both ancient divergences and recent radiations within angiosperms. Beyond their value for systematics, plastome rearrangements can reshape gene order and copy number, with measurable effects on gene expression, metabolic pathways, and photosynthetic efficiency. Evidence shows that, in certain lineages, plastid genes have been transferred to the nucleus to compensate for gene loss and preserve essential cellular functions. Looking ahead, three emerging approaches promise to deepen our understanding of plastome dynamics: comprehensive pan-plastome surveys coupled with long-read sequencing of under-sampled lineages; targeted plastid transformation to engineer specific rearrangements; and advanced genome editing to test their adaptive significance. Together, these strategies will illuminate how plastid structural change impacts plant evolution and adaptation.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144561604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unearthing the invisible: simulating maize root domestication through deep time. 挖掘无形：模拟玉米根系的深时间驯化。

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-07-04 DOI: 10.1111/nph.70376

Peng Yu

引用次数: 0