New Phytologist最新文献

Homoeologous crossovers are distally biased and underlie genomic instability in first-generation neo-allopolyploid Arabidopsis suecica. 同源交叉具有远端偏向性，是拟南芥第一代新全多倍体基因组不稳定的基础。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-09-06 DOI: 10.1111/nph.20095

Candida Nibau, Aled Evans, Holly King, Dylan Wyn Phillips, Andrew Lloyd

{"title":"Homoeologous crossovers are distally biased and underlie genomic instability in first-generation neo-allopolyploid Arabidopsis suecica.","authors":"Candida Nibau, Aled Evans, Holly King, Dylan Wyn Phillips, Andrew Lloyd","doi":"10.1111/nph.20095","DOIUrl":"https://doi.org/10.1111/nph.20095","url":null,"abstract":"First-generation polyploids often suffer from more meiotic errors and lower fertility than established wild polyploid populations. One such example is the allopolyploid model species Arabidopsis suecica which originated c. 16 000 generations ago. We present here a comparison of meiosis and its outcomes in naturally evolved and first-generation 'synthetic' A. suecica using a combination of cytological and genomic approaches. We show that while meiosis in natural lines is largely diploid-like, synthetic lines have high levels of meiotic errors including incomplete synapsis and nonhomologous crossover formation. Whole-genome re-sequencing of progeny revealed 20-fold higher levels of homoeologous exchange and eightfold higher aneuploidy originating from synthetic parents. Homoeologous exchanges showed a strong distal bias and occurred predominantly in genes, regularly generating novel protein variants. We also observed that homoeologous exchanges can generate megabase scale INDELs when occurring in regions of inverted synteny. Finally, we observed evidence of sex-specific differences in adaptation to polyploidy with higher success in reciprocal crosses to natural lines when synthetic plants were used as the female parent. Our results directly link cytological phenotypes in A. suecica with their genomic outcomes, demonstrating that homoeologous crossovers underlie genomic instability in neo-allopolyploids and are more distally biased than homologous crossovers.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":null,"pages":null},"PeriodicalIF":9.4,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142141554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How does plant chemodiversity evolve? Testing five hypotheses in one population genetic model. 植物化学多样性是如何进化的？在一个种群遗传模型中测试五个假设

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-09-05 DOI: 10.1111/nph.20096

Meike J Wittmann, Andrea Bräutigam

{"title":"How does plant chemodiversity evolve? Testing five hypotheses in one population genetic model.","authors":"Meike J Wittmann, Andrea Bräutigam","doi":"10.1111/nph.20096","DOIUrl":"https://doi.org/10.1111/nph.20096","url":null,"abstract":"Plant chemodiversity, the diversity of plant-specialized metabolites, is an important dimension of biodiversity. However, there are so far few mathematical models to test verbal hypotheses on how chemodiversity evolved. Here, we develop such a model to test predictions of five hypotheses: the 'fluctuating selection hypothesis', the 'dominance reversal hypothesis', the interaction diversity hypothesis, the synergy hypothesis, and the screening hypothesis. We build a population genetic model of a plant population attacked by herbivore species whose occurrence fluctuates over time. We study the model using mathematical analysis and individual-based simulations. As predicted by the 'dominance reversal hypothesis', chemodiversity can be maintained if alleles conferring a defense metabolite are dominant with respect to the benefits, but recessive with respect to costs. However, even smaller changes in dominance can maintain polymorphism. Moreover, our results underpin and elaborate predictions of the synergy and interaction diversity hypotheses, and, to the extent that our model can address it, the screening hypotheses. By contrast, we found only partial support for the 'fluctuating selection hypothesis'. In summary, we have developed a flexible model and tested various verbal models for the evolution of chemodiversity. Next, more mechanistic models are needed that explicitly consider the organization of metabolic pathways.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":null,"pages":null},"PeriodicalIF":9.4,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142141555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contrasting drought tolerance traits of woody plants is associated with mycorrhizal types at the global scale. 在全球范围内，木本植物不同的耐旱特性与菌根类型有关。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-09-05 DOI: 10.1111/nph.20097

Xiaorong Liu, Kailiang Yu, Hui Liu, Richard P Phillips, Pengcheng He, Xingyun Liang, Weize Tang, César Terrer, Kimberly A Novick, Emily P Bakpa, Min Zhao, Xinbo Gao, Yi Jin, Yin Wen, Qing Ye

{"title":"Contrasting drought tolerance traits of woody plants is associated with mycorrhizal types at the global scale.","authors":"Xiaorong Liu, Kailiang Yu, Hui Liu, Richard P Phillips, Pengcheng He, Xingyun Liang, Weize Tang, César Terrer, Kimberly A Novick, Emily P Bakpa, Min Zhao, Xinbo Gao, Yi Jin, Yin Wen, Qing Ye","doi":"10.1111/nph.20097","DOIUrl":"https://doi.org/10.1111/nph.20097","url":null,"abstract":"It is well-known that the mycorrhizal type of plants correlates with different modes of nutrient cycling and availability. However, the differences in drought tolerance between arbuscular mycorrhizal (AM) and ectomycorrhizal (EcM) plants remains poorly characterized. We synthesized a global dataset of four hydraulic traits associated with drought tolerance of 1457 woody species (1139 AM and 318 EcM species) at 308 field sites. We compared these traits between AM and EcM species, with evolutionary history (i.e. angiosperms vs gymnosperms), water availability (i.e. aridity index) and biomes considered as additional factors. Overall, we found that evolutionary history and biogeography influenced differences in hydraulic traits between mycorrhizal types. Specifically, we found that (1) AM angiosperms are less drought-tolerant than EcM angiosperms in wet regions or biomes, but AM gymnosperms are more drought-tolerant than EcM gymnosperms in dry regions or biomes, and (2) in both angiosperms and gymnosperms, variation in hydraulic traits as well as their sensitivity to water availability were higher in AM species than in EcM species. Our results suggest that global shifts in water availability (especially drought) may alter the biogeographic distribution and abundance of AM and EcM plants, with consequences for ecosystem element cycling and ultimately, the land carbon sink.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":null,"pages":null},"PeriodicalIF":9.4,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142141550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic and epigenetic reprogramming in response to internal and external cues by induced transposon mobilization in Moso bamboo. 毛竹通过诱导转座子调动对内部和外部线索做出的遗传和表观遗传重编程。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-09-05 DOI: 10.1111/nph.20107

Long-Hai Zou, Bailiang Zhu, Yaxin Chen, Yaping Lu, Muthusamy Ramkrishnan, Chao Xu, Xiaohong Zhou, Yiqian Ding, Jungnam Cho, Mingbing Zhou

{"title":"Genetic and epigenetic reprogramming in response to internal and external cues by induced transposon mobilization in Moso bamboo.","authors":"Long-Hai Zou, Bailiang Zhu, Yaxin Chen, Yaping Lu, Muthusamy Ramkrishnan, Chao Xu, Xiaohong Zhou, Yiqian Ding, Jungnam Cho, Mingbing Zhou","doi":"10.1111/nph.20107","DOIUrl":"https://doi.org/10.1111/nph.20107","url":null,"abstract":"Long terminal repeat retroelements (LTR-REs) have profound effects on DNA methylation and gene regulation. Despite the vast abundance of LTR-REs in the genome of Moso bamboo (Phyllostachys edulis), an industrial crop in underdeveloped countries, their precise implication of the LTR-RE mobility in stress response and development remains unknown. We investigated the RNA and DNA products of LTR-REs in Moso bamboo under various developmental stages and stressful conditions. Surprisingly, our analyses identified thousands of active LTR-REs, particularly those located near genes involved in stress response and developmental regulation. These genes adjacent to active LTR-REs exhibited an increased expression under stress and are associated with reduced DNA methylation that is likely affected by the induced LTR-REs. Moreover, the analyses of simultaneous mapping of insertions and DNA methylation showed that the LTR-REs effectively alter the epigenetic status of the genomic regions where they inserted, and concomitantly their transcriptional competence which might impact the stress resilience and growth of the host. Our work unveils the unusually strong LTR-RE mobility in Moso bamboo and its close association with (epi)genetic changes, which supports the co-evolution of the parasitic DNAs and host genome in attaining stress tolerance and developmental robustness.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":null,"pages":null},"PeriodicalIF":9.4,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142141551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GLABRA2 transcription factor integrates arsenic tolerance with epidermal cell fate determination. GLABRA2 转录因子将砷耐受性与表皮细胞命运决定结合在一起。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-09-05 DOI: 10.1111/nph.20099

Micaela Andrea Navarro, Cristina Navarro, Luis Eduardo Hernández, María Garnica, José Manuel Franco-Zorrilla, Yogev Burko, Sara González-Serrano, José M García-Mina, José Pruneda-Paz, Joanne Chory, Antonio Leyva

{"title":"GLABRA2 transcription factor integrates arsenic tolerance with epidermal cell fate determination.","authors":"Micaela Andrea Navarro, Cristina Navarro, Luis Eduardo Hernández, María Garnica, José Manuel Franco-Zorrilla, Yogev Burko, Sara González-Serrano, José M García-Mina, José Pruneda-Paz, Joanne Chory, Antonio Leyva","doi":"10.1111/nph.20099","DOIUrl":"https://doi.org/10.1111/nph.20099","url":null,"abstract":"Arsenic poses a global threat to living organisms, compromising crop security and yield. Limited understanding of the transcriptional network integrating arsenic-tolerance mechanisms with plant developmental responses hinders the development of strategies against this toxic metalloid. Here, we conducted a high-throughput yeast one-hybrid assay using as baits the promoter region from the arsenic-inducible genes ARQ1 and ASK18 from Arabidopsis thaliana, coupled with a transcriptomic analysis, to uncover novel transcriptional regulators of the arsenic response. We identified the GLABRA2 (GL2) transcription factor as a novel regulator of arsenic tolerance, revealing a wider regulatory role beyond its established function as a repressor of root hair formation. Furthermore, we found that ANTHOCYANINLESS2 (ANL2), a GL2 subfamily member, acts redundantly with this transcription factor in the regulation of arsenic signaling. Both transcription factors act as repressors of arsenic response. gl2 and anl2 mutants exhibit enhanced tolerance and reduced arsenic accumulation. Transcriptional analysis in the gl2 mutant unveils potential regulators of arsenic tolerance. These findings highlight GL2 and ANL2 as novel integrators of the arsenic response with developmental outcomes, offering insights for developing safer crops with reduced arsenic content and increased tolerance to this hazardous metalloid.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":null,"pages":null},"PeriodicalIF":9.4,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142141552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

H₂ ¹⁸O vapour labelling reveals evidence of radial Péclet effects, but in not all leaves. H2 18O 蒸汽标记显示了径向贝克莱特效应，但并非所有叶片都有这种效应。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-09-05 DOI: 10.1111/nph.20087

Margaret M Barbour, Melissa A White, Lulu Liu

{"title":"H2 18O vapour labelling reveals evidence of radial Péclet effects, but in not all leaves.","authors":"Margaret M Barbour, Melissa A White, Lulu Liu","doi":"10.1111/nph.20087","DOIUrl":"https://doi.org/10.1111/nph.20087","url":null,"abstract":"Contradictory evidence exists regarding the relevance of Péclet-like gradients in leaf water isotopes, making it difficult to accurately predict variation in isotope composition. Here, we use H2 18O vapour labelling to directly test whether leaf water isotopes diffuse back into the xylem to be carried forward to more distal leaf portions. Backward diffusion has been assumed, due to observations of increasing enrichment towards the tip and outer edges of some leaves. Further complicating the selection of leaf water isotope models is the observation that some, but not all, leaves demonstrate a radial Péclet effect in bulk leaf water and that the hydraulic design of leaves may influence the development of isotope gradients in leaves. Carry-forward of H2 18O vapour label was detected in the two monocot species assessed (oat and corn), but not in the two dicot species (foxglove and sunflower). Further, bulk leaf water measurements at differing transpiration rates indicated that a bulk leaf water Péclet effect was relevant for foxglove only. We conclude that both leaf hydraulic design and relative velocities of water within transport pathways influence leaf water isotope composition, reconciling seemingly contradictory previous results regarding the relevance of Péclet effects to leaf water isotopes.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":null,"pages":null},"PeriodicalIF":9.4,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142141553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shorting the metaphorical circuit: vascular partitioning and stomatal patchiness can create apparent unsaturation and CO₂ gradient inversion in the Ohmic analogy for leaf gas exchange. 隐喻电路的短路：在叶片气体交换的欧姆类比中，维管分区和气孔斑块会造成明显的不饱和和二氧化碳梯度反转。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-09-05 DOI: 10.1111/nph.20106

Fulton E Rockwell

{"title":"Shorting the metaphorical circuit: vascular partitioning and stomatal patchiness can create apparent unsaturation and CO2 gradient inversion in the Ohmic analogy for leaf gas exchange.","authors":"Fulton E Rockwell","doi":"10.1111/nph.20106","DOIUrl":"https://doi.org/10.1111/nph.20106","url":null,"abstract":"Analyses of leaf gas exchange rely on an Ohmic analogy that arrays single stomatal, internal air space, and mesophyll conductances in series. Such models underlie inferences of mesophyll conductance and the relative humidity of leaf airspaces, reported to fall as low as 80%. An unresolved question is whether such series models are biased with respect to real leaves, whose internal air spaces are chambered at various scales by vasculature. To test whether unsaturation could emerge from modeling artifacts, we compared series model estimates with true parameter values for a chambered leaf with varying distributions and magnitudes of leaf surface conductance ('patchiness'). Distributions of surface conductance can create large biases in gas exchange calculations. Both apparent unsaturation and internal CO2 gradient inversion can be produced by the evolution of broader distributions of stomatal apertures consistent with a decrease in surface conductance, as might occur under increasing vapor pressure deficit. In gas exchange experiments, the behaviors of derived quantities defined by simple series models are highly sensitive to the true partitioning of flux and stomatal apertures across leaf surfaces. New methods are needed to disentangle model artifacts from real biological responses.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":null,"pages":null},"PeriodicalIF":9.4,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142141556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AtALMT5 mediates vacuolar fumarate import and regulates the malate/fumarate balance in Arabidopsis. AtALMT5 在拟南芥中介导液泡富马酸的输入并调节苹果酸/富马酸的平衡。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-09-05 DOI: 10.1111/nph.20077

Roxane Doireau, Justyna Jaślan, Paloma Cubero-Font, Elsa Demes-Causse, Karen Bertaux, Cédric Cassan, Pierre Pétriarcq, Alexis De Angeli

{"title":"AtALMT5 mediates vacuolar fumarate import and regulates the malate/fumarate balance in Arabidopsis.","authors":"Roxane Doireau, Justyna Jaślan, Paloma Cubero-Font, Elsa Demes-Causse, Karen Bertaux, Cédric Cassan, Pierre Pétriarcq, Alexis De Angeli","doi":"10.1111/nph.20077","DOIUrl":"https://doi.org/10.1111/nph.20077","url":null,"abstract":"Malate and fumarate constitute a significant fraction of the carbon fixed by photosynthesis, and they are at the crossroad of central metabolic pathways. In Arabidopsis thaliana, they are transiently stored in the vacuole to keep cytosolic homeostasis. The malate and fumarate transport systems of the vacuolar membrane are key players in the control of cell metabolism. Notably, the molecular identity of these transport systems remains mostly unresolved. We used a combination of imaging, electrophysiology and molecular physiology to identify an important molecular actor of dicarboxylic acid transport across the tonoplast. Here, we report the function of the A. thaliana Aluminium-Activated Malate Transporter 5 (AtALMT5). We characterised its ionic transport properties, expression pattern, localisation and function in vivo. We show that AtALMT5 is expressed in photosynthetically active tissues and localised in the tonoplast. Patch-clamp and in planta analyses demonstrated that AtALMT5 is an ion channel-mediating fumarate loading of the vacuole. We found in almt5 plants a reduced accumulation of fumarate in the leaves, in parallel with increased malate concentrations. These results identified AtALMT5 as an ion channel-mediating fumarate transport in the vacuoles of mesophyll cells and regulating the malate/fumarate balance in Arabidopsis.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":null,"pages":null},"PeriodicalIF":9.4,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142141549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progressing beyond colonization strategies to understand arbuscular mycorrhizal fungal life history. 超越定殖策略，了解丛枝菌根真菌的生活史。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-09-04 DOI: 10.1111/nph.20090

Tessa Camenzind, Carlos A Aguilar-Trigueros, Meike K Heuck, Solomon Maerowitz-McMahan, Matthias C Rillig, Will K Cornwell, Jeff R Powell

{"title":"Progressing beyond colonization strategies to understand arbuscular mycorrhizal fungal life history.","authors":"Tessa Camenzind, Carlos A Aguilar-Trigueros, Meike K Heuck, Solomon Maerowitz-McMahan, Matthias C Rillig, Will K Cornwell, Jeff R Powell","doi":"10.1111/nph.20090","DOIUrl":"https://doi.org/10.1111/nph.20090","url":null,"abstract":"Knowledge of differential life-history strategies in arbuscular mycorrhizal (AM) fungi is relevant for understanding the ecology of this group and its potential role in sustainable agriculture and carbon sequestration. At present, AM fungal life-history theories often focus on differential investment into intra- vs extraradical structures among AM fungal taxa, and its implications for plant benefits. With this Viewpoint we aim to expand these theories by integrating a mycocentric economics- and resource-based life-history framework. As in plants, AM fungal carbon and nutrient demands are stoichiometrically coupled, though uptake of these elements is spatially decoupled. Consequently, investment in morphological structures for carbon vs nutrient uptake is not in competition. We argue that understanding the ecology and evolution of AM fungal life-history trade-offs requires increased focus on variation among structures foraging for the same element, that is within intra- or extraradical structures (in our view a 'horizontal' axis), not just between them ('vertical' axis). Here, we elaborate on this argument and propose a range of plausible life-history trade-offs that could lead to the evolution of strategies in AM fungi, providing testable hypotheses and creating opportunities to explain AM fungal co-existence, and the context-dependent effects of AM fungi on plant growth and soil carbon dynamics.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":null,"pages":null},"PeriodicalIF":9.4,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142127080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impaired Brown midrib12 function orchestrates sorghum resistance to aphids via an auxin conjugate indole-3-acetic acid-aspartic acid. 布朗中肋12功能受损，通过吲哚-3-乙酸-天冬氨酸共轭辅酶协调高粱对蚜虫的抗性。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-09-04 DOI: 10.1111/nph.20091

Sajjan Grover, De-Fen Mou, Kumar Shrestha, Heena Puri, Lise Pingault, Scott E Sattler, Joe Louis

{"title":"Impaired Brown midrib12 function orchestrates sorghum resistance to aphids via an auxin conjugate indole-3-acetic acid-aspartic acid.","authors":"Sajjan Grover, De-Fen Mou, Kumar Shrestha, Heena Puri, Lise Pingault, Scott E Sattler, Joe Louis","doi":"10.1111/nph.20091","DOIUrl":"https://doi.org/10.1111/nph.20091","url":null,"abstract":"Lignin, a complex heterogenous polymer present in virtually all plant cell walls, plays a critical role in protecting plants from various stresses. However, little is known about how lignin modifications in sorghum will impact plant defense against sugarcane aphids (SCA), a key pest of sorghum. We utilized the sorghum brown midrib (bmr) mutants, which are impaired in monolignol synthesis, to understand sorghum defense mechanisms against SCA. We found that loss of Bmr12 function and overexpression (OE) of Bmr12 provided enhanced resistance and susceptibility to SCA, respectively, as compared with wild-type (WT; RTx430) plants. Monitoring of the aphid feeding behavior indicated that SCA spent more time in reaching the first sieve element phase on bmr12 plants compared with RTx430 and Bmr12-OE plants. A combination of transcriptomic and metabolomic analyses revealed that bmr12 plants displayed altered auxin metabolism upon SCA infestation and specifically, elevated levels of auxin conjugate indole-3-acetic acid-aspartic acid (IAA-Asp) were observed in bmr12 plants compared with RTx430 and Bmr12-OE plants. Furthermore, exogenous application of IAA-Asp restored resistance in Bmr12-OE plants, and artificial diet aphid feeding trial bioassays revealed that IAA-Asp is associated with enhanced resistance to SCA. Our findings highlight the molecular underpinnings that contribute to sorghum bmr12-mediated resistance to SCA.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":null,"pages":null},"PeriodicalIF":9.4,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142134268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0