New Phytologist最新文献_第2页

The DNA N6-adenine methylation target gene GhMAF1 promotes fiber initiation at the base of Gossypium ovules.

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-03-10 DOI: 10.1111/nph.70047

Yanfei Pei, Yongming Liu, Jun Peng, Yayue Pei, Tianen Zhang, Pengfei Miao, Yuanyuan Liu, Yang Liu, Ji Liu, Zuoren Yang, Fuguang Li, Zhi Wang

{"title":"The DNA N6-adenine methylation target gene GhMAF1 promotes fiber initiation at the base of Gossypium ovules.","authors":"Yanfei Pei, Yongming Liu, Jun Peng, Yayue Pei, Tianen Zhang, Pengfei Miao, Yuanyuan Liu, Yang Liu, Ji Liu, Zuoren Yang, Fuguang Li, Zhi Wang","doi":"10.1111/nph.70047","DOIUrl":"https://doi.org/10.1111/nph.70047","url":null,"abstract":"DNA methylation consists of 5-methylcytosine and N6-methyl deoxyadenosine (6mA) and is crucial in plant development. However, its specific role and potential mechanism to initiate cotton fibers remain unclear. This study employed Oxford Nanopore Technologies (ONT) sequencing to analyze DNA methylation alterations in ZM24 and ZM24 fuzzless-lintless (ZM24fl) during fiber initiation. Our results indicated that DNA 6mA methylation exhibited the most remarkable difference among ovule samples at -2, 0, and 5 d post anthesis of ZM24 and ZM24fl. Subsequently, genes with significant changes in DNA 6mA methylation and transcription during fiber initiation were screened. We found that GhMAF1 displayed significant transcriptional upregulation and 6mA enrichment in its promoter, which could serve as a potential target for DNA 6mA in fiber initiation. Further, we knocked out GhMAF1 using CRISPR-Cas technology and demonstrated that GhMAF1 specifically promotes the initiation of fiber cells at the base of the ovule by mediating the downstream JAZ2/CPC-MML3/MML4 pathway. These findings unveil a novel spatial module of fiber cell initiation on the ovule surface that involves GhMAF1. Ultimately, this work provides significant knowledge for the regulatory network of DNA 6mA modification in fiber initiation to improve fiber yield and quality.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143598105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mesophyll conductance and cell wall composition: insights from a meta-analysis across species.

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-02-27 DOI: 10.1111/nph.70024

Coralie E Salesse-Smith, Yi Xiao

引用次数: 0

Strigolactone and gibberellin crosstalk: the role of the SLR1-OsMADS23-D14 module in regulating rice tiller development.

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-02-19 DOI: 10.1111/nph.20444

Hui Zhuang, Yun-Feng Li

引用次数: 0

New insights into the leaf economic spectrum could benefit terrestrial models.

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-01-31 DOI: 10.1111/nph.20419

Anna B Harper, Simon Jones

引用次数: 0

Off-target drift of the herbicide dicamba disrupts plant-pollinator interactions via novel pathways.

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-01-31 DOI: 10.1111/nph.20438

Regina S Baucom, Veronica Iriart, Anah Soble, Matthew R Armstrong, Tia-Lynn Ashman

{"title":"Off-target drift of the herbicide dicamba disrupts plant-pollinator interactions via novel pathways.","authors":"Regina S Baucom, Veronica Iriart, Anah Soble, Matthew R Armstrong, Tia-Lynn Ashman","doi":"10.1111/nph.20438","DOIUrl":"10.1111/nph.20438","url":null,"abstract":"While herbicide use in agriculture is expected to have many effects on surrounding weed communities, it is largely unknown how plant exposure to sublethal doses of herbicide may subsequently impact plant-pollinator interactions. We tested the hypothesis that sublethal herbicide exposure indirectly alters plant-pollinator interactions through changes in plant traits, and specifically through alterations in floral display. Using a common garden experiment, we exposed 11 weed species to the herbicide dicamba and examined the potential for changes in pollinator abundance and patterns of pollinator visitation as well as alterations to plant traits. We found variation among plant species in the extent of damage from dicamba drift, and variation in size, flowering time, and flower displays, with some plant species showing negative impacts and others showing little effect. Pollinator frequencies were reduced in dicamba-exposed plots, and pollinator visits were reduced for some weed species yet not for others. Structural equation modeling revealed that the relationship between flower display and pollinator visits was disrupted in the presence of dicamba. Our study provides the most comprehensive picture to date of the impacts of herbicide drift on plant-pollinator interactions, with findings that highlight an underappreciated role of services supplied by weedy communities.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143069095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LsKN1 and LsOFP6 synergistically regulate the bolting time by modulating the gibberellin pathway in lettuce. LsKN1和LsOFP6通过调控生菜赤霉素途径协同调控抽苔时间。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-11-29 DOI: 10.1111/nph.20307

Yetong Qi, Wei Shao, Haoyu Chen, Temoor Ahmed, Xinhui Zhao, Yong Wang, Lei Zhu, Shouru Sun, Hanhui Kuang, Guanghui An

{"title":"LsKN1 and LsOFP6 synergistically regulate the bolting time by modulating the gibberellin pathway in lettuce.","authors":"Yetong Qi, Wei Shao, Haoyu Chen, Temoor Ahmed, Xinhui Zhao, Yong Wang, Lei Zhu, Shouru Sun, Hanhui Kuang, Guanghui An","doi":"10.1111/nph.20307","DOIUrl":"https://doi.org/10.1111/nph.20307","url":null,"abstract":"Bolting time is an important agronomic trait in lettuce (Lactuca sativa) production. Premature bolting significantly reduces crop quality and marketability. Here, we report map-based cloning and characterization of a LsKN1 gene that controls bolting in lettuce. A segregating population was developed by crossing a crisphead-type cultivar with a stem-type cultivar to genetically map and clone the LsKN1 gene. In the late-bolting parent (crisphead), the LsKN1 was activated by a CACTA-like transposon which was inserted into the first exon of LsKN1. Complementation test, overexpression, and CRISPR/cas9 knockout showed that the activated LsKN1 allele (LsKN1TP) delays bolting in lettuce. ChIP-seq and phytohormone analysis demonstrated that LsKN1 regulates gibberellin (GA) biosynthesis and response. LsKN1TP binds to the promoter of the LsGA20ox1 and LsRGA1, and down- and upregulates their expression, respectively. Furthermore, LsRGA1 interacts with LsKN1TP to enhance the repression of GA biosynthesis. LsOFP6 acts as a safeguard, interacting with LsKN1TP to prevent excessive inhibition of GA biosynthesis and response during the vegetative-to-reproductive phase transition. The LsKN1-LsOFP6 module orchestrates the GA pathway to regulate bolting time in lettuce, which provides insight into the bolting development in lettuce and offers valuable genetic resources for breeding lettuce varieties resistant to premature bolting.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":9.4,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142752072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A fungal sRNA silences a host plant transcription factor to promote arbuscular mycorrhizal symbiosis. 真菌的 sRNA 能抑制宿主植物的转录因子，促进丛枝菌根共生。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-11-18 DOI: 10.1111/nph.20273

Alessandro Silvestri, William Conrad Ledford, Valentina Fiorilli, Cristina Votta, Alessia Scerna, Jacopo Tucconi, Antonio Mocchetti, Gianluca Grasso, Raffaella Balestrini, Hailing Jin, Ignacio Rubio-Somoza, Luisa Lanfranco

{"title":"A fungal sRNA silences a host plant transcription factor to promote arbuscular mycorrhizal symbiosis.","authors":"Alessandro Silvestri, William Conrad Ledford, Valentina Fiorilli, Cristina Votta, Alessia Scerna, Jacopo Tucconi, Antonio Mocchetti, Gianluca Grasso, Raffaella Balestrini, Hailing Jin, Ignacio Rubio-Somoza, Luisa Lanfranco","doi":"10.1111/nph.20273","DOIUrl":"https://doi.org/10.1111/nph.20273","url":null,"abstract":"Cross-kingdom RNA interference (ckRNAi) is a mechanism of interspecies communication where small RNAs (sRNAs) are transported from one organism to another; these sRNAs silence target genes in trans by loading into host AGO proteins. In this work, we investigated the occurrence of ckRNAi in Arbuscular Mycorrhizal Symbiosis (AMS). We used an in silico prediction analysis to identify a sRNA (Rir2216) from the AM fungus Rhizophagus irregularis and its putative plant gene target, the Medicago truncatula MtWRKY69 transcription factor. Heterologous co-expression assays in Nicotiana benthamiana, 5' RACE reactions and AGO1-immunoprecipitation assays from mycorrhizal roots were used to characterize the Rir2216-MtWRKY69 interaction. We further analyzed MtWRKY69 expression profile and the contribution of constitutive and conditional MtWRKY69 expression to AMS. We show that Rir2216 is loaded into an AGO1 silencing complex from the host plant M. truncatula, leading to cleavage of a host target transcript encoding for the MtWRKY69 transcription factor. MtWRKY69 is specifically downregulated in arbusculated cells in mycorrhizal roots and increased levels of MtWRKY69 expression led to a reduced AM colonization level. Our results indicate that MtWRKY69 silencing, mediated by a fungal sRNA, is relevant for AMS; we thus present the first experimental evidence of fungus to plant ckRNAi in AMS.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":9.4,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complex consequences of disturbance on canopy plant communities of world forests: a review and synthesis 干扰对世界森林冠层植物群落的复杂影响：综述和综合。

IF 9.4 1区生物学

New Phytologist Pub Date : 2023-10-10 DOI: 10.1111/nph.19245

Nalini M. Nadkarni

{"title":"Complex consequences of disturbance on canopy plant communities of world forests: a review and synthesis","authors":"Nalini M. Nadkarni","doi":"10.1111/nph.19245","DOIUrl":"10.1111/nph.19245","url":null,"abstract":"Epiphytes and their associated biota are increasingly recognized as contributing to biodiversity and to filling critical ecosystem functions in world forests. However, the attributes that have made them successful in canopy environments also make them vulnerable to natural and human-induced disturbances. Drawing upon ecological frameworks to understand disturbance, I categorized and synthesized the drivers and the consequences of disturbances on epiphytic materials. Across all impacts, disturbance agents were significantly more likely to lead to negative, rather than positive, effects in both tropical and temperate locales. Significantly more studies reported negative effects on abundance, diversity, community composition and connectivity, but some studies showed that disturbances enhanced these attributes. Although particular disturbance agents did not differently influence individual consequences, they explained a significant portion of variation in aggregated totals. Surprisingly, relative to human disturbances, natural disturbances were more likely to lead to negative effects. Many studies provided recommendations for effective societal responses to mitigate negative impacts, such as retaining large, old trees in forestry operations, patch-clearing for epiphyte harvest, maximizing forest fragment size, using epiphytes as bioindicators of disturbance, and applying principles of community forestry to land management. Future actions should also include communication of these results to policymakers and land managers.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":"240 4","pages":"1366-1380"},"PeriodicalIF":9.4,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.19245","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41216833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tackling redundancy: genetic mechanisms underlying paralog compensation in plants 处理冗余：植物同源补偿的遗传机制。

IF 9.4 1区生物学

New Phytologist Pub Date : 2023-09-19 DOI: 10.1111/nph.19267

Sessen Daniel Iohannes, David Jackson

引用次数: 0

Deductive automated pollen classification in environmental samples via exploratory deep learning and imaging flow cytometry 通过探索性深度学习和成像流式细胞术对环境样本中的花粉进行演绎式自动分类

IF 9.4 1区生物学

New Phytologist Pub Date : 2023-09-07 DOI: 10.1111/nph.19186

Claire M. Barnes, Ann L. Power, Daniel G. Barber, Richard K. Tennant, Richard T. Jones, G. Rob Lee, Jackie Hatton, Angela Elliott, Joana Zaragoza-Castells, Stephen M. Haley, Huw D. Summers, Minh Doan, Anne E. Carpenter, Paul Rees, John Love

引用次数: 0