New Phytologist最新文献_第2页

Arabidopsis enters the single-cell proteomics era. 拟南芥进入单细胞蛋白质组学时代。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-12-01 Epub Date: 2024-07-22 DOI: 10.1111/nph.19992

Monique van Schie, Dolf Weijers

引用次数: 0

Shorting the metaphorical circuit: vascular partitioning and stomatal patchiness can create apparent unsaturation and CO₂ gradient inversion in the Ohmic analogy for leaf gas exchange. 隐喻电路的短路：在叶片气体交换的欧姆类比中，维管分区和气孔斑块会造成明显的不饱和和二氧化碳梯度反转。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-12-01 Epub Date: 2024-09-05 DOI: 10.1111/nph.20106

Fulton E Rockwell

{"title":"Shorting the metaphorical circuit: vascular partitioning and stomatal patchiness can create apparent unsaturation and CO2 gradient inversion in the Ohmic analogy for leaf gas exchange.","authors":"Fulton E Rockwell","doi":"10.1111/nph.20106","DOIUrl":"10.1111/nph.20106","url":null,"abstract":"Analyses of leaf gas exchange rely on an Ohmic analogy that arrays single stomatal, internal air space, and mesophyll conductances in series. Such models underlie inferences of mesophyll conductance and the relative humidity of leaf airspaces, reported to fall as low as 80%. An unresolved question is whether such series models are biased with respect to real leaves, whose internal air spaces are chambered at various scales by vasculature. To test whether unsaturation could emerge from modeling artifacts, we compared series model estimates with true parameter values for a chambered leaf with varying distributions and magnitudes of leaf surface conductance ('patchiness'). Distributions of surface conductance can create large biases in gas exchange calculations. Both apparent unsaturation and internal CO2 gradient inversion can be produced by the evolution of broader distributions of stomatal apertures consistent with a decrease in surface conductance, as might occur under increasing vapor pressure deficit. In gas exchange experiments, the behaviors of derived quantities defined by simple series models are highly sensitive to the true partitioning of flux and stomatal apertures across leaf surfaces. New methods are needed to disentangle model artifacts from real biological responses.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":null,"pages":null},"PeriodicalIF":9.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142141556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An activity-based sensing fluorogenic probe for monitoring O-methyltransferase in plants. 用于监测植物中 O-甲基转移酶的基于活性的荧光探针。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-12-01 Epub Date: 2024-09-04 DOI: 10.1111/nph.20104

Xin-Lei Jia, Lixiang Zhu, Yuanyu Li, Pan Zhang, Xiao Chen, Kai Shao, Jingxian Feng, Shi Qiu, Jiaran Geng, Yingbo Yang, Zongtai Wu, Jingshi Xue, Ping Wang, Wansheng Chen, Ying Xiao

{"title":"An activity-based sensing fluorogenic probe for monitoring O-methyltransferase in plants.","authors":"Xin-Lei Jia, Lixiang Zhu, Yuanyu Li, Pan Zhang, Xiao Chen, Kai Shao, Jingxian Feng, Shi Qiu, Jiaran Geng, Yingbo Yang, Zongtai Wu, Jingshi Xue, Ping Wang, Wansheng Chen, Ying Xiao","doi":"10.1111/nph.20104","DOIUrl":"10.1111/nph.20104","url":null,"abstract":"Activity-based sensing probes are powerful tools for monitoring enzymatic activities in complex biological samples such as cellular and live animals; however, their application in plants remains challenging. Herein, fourteen activity-based fluorescent probes were assayed against Arabidopsis O-methyltransferases (AtOMTs). One probe, 3-BTD, displayed a high selectivity, reactivity, and fluorescence response toward AtOMTs especially the isoform AtCCoAOMT. We further characterized the features of this probe and explored whether it could be used to detect OMT activities in living plant cells. Our results show that 3-BTD can be used to visualize OMT activity in Arabidopsis, and no fluorescent signal was observed in the comt/ccoaomt double mutant, indicating that it has good specificity. Interestingly, in contrast to the observation that AtCCoAOMT-YFP accumulated in both cytoplasm and nucleus, OMT enzymatic activity tracked by 3-BTD probe was found only in the cytoplasm. This underscores the importance of activity-based sensing in studying protein function. Moreover, 3-BTD can be successfully applied in OMT visualization of different plants. This study indicates that 3-BTD can serve as a potential probe for in situ monitoring the real activity of OMT in multiple plants and provides a strategy for visualizing the activity of other enzymes in plants.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":null,"pages":null},"PeriodicalIF":9.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142134267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mapping of drought-induced changes in tissue characteristics across the leaf profile of Populus balsamifera. 绘制杨树叶片轮廓中由干旱引起的组织特征变化图。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-11-06 DOI: 10.1111/nph.20240

Mina Momayyezi, Cheyenne Chu, Jarvis A Stobbs, Raju Y Soolanayakanahally, Robert D Guy, Andrew J McElrone, Thorsten Knipfer

{"title":"Mapping of drought-induced changes in tissue characteristics across the leaf profile of Populus balsamifera.","authors":"Mina Momayyezi, Cheyenne Chu, Jarvis A Stobbs, Raju Y Soolanayakanahally, Robert D Guy, Andrew J McElrone, Thorsten Knipfer","doi":"10.1111/nph.20240","DOIUrl":"10.1111/nph.20240","url":null,"abstract":"Leaf architecture impacts the ease of gases diffusion, biochemical process, and photosynthetic performance. For balsam poplar, a widespread North American species, the influence of water availability on leaf anatomy and subsequent photosynthetic performance remains unknown. To address this shortcoming, we characterized the anatomical changes across the leaf profile in three-dimensional space for saplings subjected to soil drying and rewatering using X-ray microcomputed tomography. Our hypothesis was that higher abundance of bundle sheet extensions (BSE) minimizes drought-induced changes in intercellular airspace volume relative to mesophyll volume (i.e. mesophyll porosity, θIAS) and aids recovery by supporting leaf structural integrity. Leaves of 'Carnduff-9' with less abundant BSEs exhibited greater θIAS, higher spongy mesophyll surface area, reduced palisade mesophyll surface area, and less veins compared with 'Gillam-5'. Under drought conditions, Carnduff-9 showed significant changes in θIAS across leaf profile while that was little for 'Gillam-5'. Under rewatered conditions, drought-induced changes in θIAS were fully reversible in 'Gillam-5' but not in 'Carnduff-9'. Our data suggest that a 'robust' leaf structure with higher abundance of BSEs, reduced θIAS, and relatively large mesophyll surface area provides for improved photosynthetic capacity under drought and supports recovery in leaf architecture after rewatering in balsam poplar.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":null,"pages":null},"PeriodicalIF":9.4,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

StCDF1: A 'jack of all trades' clock output with a central role in regulating potato nitrate reduction activity. StCDF1：万能 "时钟输出，在调节马铃薯硝酸盐还原活性方面发挥核心作用。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-11-06 DOI: 10.1111/nph.20186

Maroof Ahmed Shaikh, Lorena Ramírez-Gonzales, José M Franco-Zorrilla, Evyatar Steiner, Marian Oortwijn, Christian W B Bachem, Salomé Prat

{"title":"StCDF1: A 'jack of all trades' clock output with a central role in regulating potato nitrate reduction activity.","authors":"Maroof Ahmed Shaikh, Lorena Ramírez-Gonzales, José M Franco-Zorrilla, Evyatar Steiner, Marian Oortwijn, Christian W B Bachem, Salomé Prat","doi":"10.1111/nph.20186","DOIUrl":"https://doi.org/10.1111/nph.20186","url":null,"abstract":"Transcription factors of the CYCLING DOF FACTOR (CDF) family activate in potato the SP6A FT tuberization signal in leaves. In modern cultivars, truncated StCDF1.2 alleles override strict SD control by stabilizing the StCDF1 protein, which leads to StCOL1 suppression and impaired activation of the antagonic SP5G paralog. By using DAP-seq and RNA-seq studies, we here show that StCDF1 not only acts as an upstream regulator of the day length pathway but also directly regulates several N assimilation and transport genes. StCDF1 directly represses expression of NITRATE REDUCTASE (NR/NIA), which catalyses the first reduction step in nitrate assimilation, and is encoded by a single potato locus. StCDF1 knock-down lines performed better in N-limiting conditions, and this phenotype correlated with derepressed StNR expression. Also, deletion of the StNR DAP-seq region abolished repression by StCDF1, while it did not affect NLP7-dependent activation of the StNR promoter. We identified multiple nucleotide polymorphisms in the DAP-seq region in potato cultivars with early StCDF1 alleles, suggesting that this genetic variation was selected as compensatory mechanism to the negative impact of StCDF1 stabilization. Thereby, directed modification of the StCDF1-recognition elements emerges as a promising strategy to enhance limiting StNR activity in potato.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":null,"pages":null},"PeriodicalIF":9.4,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From synthetic communities to synthetic ecosystems: exploring causalities in plant-microbe-environment interactions. 从合成群落到合成生态系统：探索植物-微生物-环境相互作用的因果关系。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-11-05 DOI: 10.1111/nph.20250

Guillaume Chesneau, Johannes Herpell, Rubén Garrido-Oter, Stéphane Hacquard

引用次数: 0

New insights into a sensitive life stage: hydraulics of tree seedlings in their first growing season. 对敏感生命阶段的新认识：树苗第一个生长季节的水力学。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-11-05 DOI: 10.1111/nph.20243

Barbara Beikircher, Magdalena Held, Adriano Losso, Stefan Mayr

{"title":"New insights into a sensitive life stage: hydraulics of tree seedlings in their first growing season.","authors":"Barbara Beikircher, Magdalena Held, Adriano Losso, Stefan Mayr","doi":"10.1111/nph.20243","DOIUrl":"https://doi.org/10.1111/nph.20243","url":null,"abstract":"The first year in a tree's life is characterized by distinct morphological changes, requiring constant adjustments of the hydraulic system. Despite their importance for the natural regeneration of forests and future vegetation composition, little has been known about the hydraulics of tree seedlings. At different times across the first growing season, we analysed xylem area-specific (Kshoot_Axyl) and leaf area-specific (Kshoot_L) shoot hydraulic conductance, as well as embolism resistance of three temperate conifer trees, two angiosperm trees and one angiosperm shrub, and related findings to cell osmotic parameters and xylem anatomical traits. Over the first 10 wk after germination, Kshoot_Axyl and Kshoot_L sharply decreased, then remained stable until the end of the growing season. Embolism resistance was remarkably low in the youngest stages but, coupled with an increase in cell wall reinforcement, significantly increased towards autumn. Contemporaneously, water potential at turgor loss and osmotic potential at saturation decreased. Independent of lineage, species and growth form, the transition from primary to secondary xylem resulted in a less efficient but increasingly more embolism-resistant hydraulic system, enabling stable water supply under increasing risk for low water potentials.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":null,"pages":null},"PeriodicalIF":9.4,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The first intron and promoter of Arabidopsis DIACYLGLYCEROL ACYLTRANSFERASE 1 exert synergistic effects on pollen and embryo lipid accumulation. 拟南芥二乙酰甘油 ACYLTRANSFERASE 1 的第一个内含子和启动子对花粉和胚胎脂质积累有协同作用。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-11-05 DOI: 10.1111/nph.20244

Sean T McGuire, Jay Shockey, Philip D Bates

{"title":"The first intron and promoter of Arabidopsis DIACYLGLYCEROL ACYLTRANSFERASE 1 exert synergistic effects on pollen and embryo lipid accumulation.","authors":"Sean T McGuire, Jay Shockey, Philip D Bates","doi":"10.1111/nph.20244","DOIUrl":"https://doi.org/10.1111/nph.20244","url":null,"abstract":"Accumulation of triacylglycerols (TAGs) is crucial during various stages of plant development. In Arabidopsis, two enzymes share overlapping functions to produce TAGs, namely acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) and phospholipid:diacylglycerol acyltransferase 1 (PDAT1). Loss of function of both genes in a dgat1-1/pdat1-2 double mutant is gametophyte lethal. However, the key regulatory elements controlling tissue-specific expression of either gene has not yet been identified. We transformed a dgat1-1/dgat1-1//PDAT1/pdat1-2 parent with transgenic constructs containing the Arabidopsis DGAT1 promoter fused to the AtDGAT1 open reading frame either with or without the first intron. Triple homozygous plants were obtained, however, in the absence of the DGAT1 first intron anthers fail to fill with pollen, seed yield is c. 10% of wild-type, seed oil content remains reduced (similar to dgat1-1/dgat1-1), and non-Mendelian segregation of the PDAT1/pdat1-2 locus occurs. Whereas plants expressing the AtDGAT1pro:AtDGAT1 transgene containing the first intron mostly recover phenotypes to wild-type. This study establishes that a combination of the promoter and first intron of AtDGAT1 provides the proper context for temporal and tissue-specific expression of AtDGAT1 in pollen. Furthermore, we discuss possible mechanisms of intron mediated regulation and how regulatory elements can be used as genetic tools to functionally replace TAG biosynthetic enzymes in Arabidopsis.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":null,"pages":null},"PeriodicalIF":9.4,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Asymmetric genome merging leads to gene expression novelty through nucleo-cytoplasmic disruptions and transcriptomic shock in Chlamydomonas triploids. 不对称基因组合并通过衣藻三倍体中的核-细胞质破坏和转录组冲击导致基因表达的新颖性。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-11-05 DOI: 10.1111/nph.20249

Lucas Prost-Boxoen, Quinten Bafort, Antoine Van de Vloet, Fabricio Almeida-Silva, Yunn Thet Paing, Griet Casteleyn, Sofie D'hondt, Olivier De Clerck, Yves Van de Peer

{"title":"Asymmetric genome merging leads to gene expression novelty through nucleo-cytoplasmic disruptions and transcriptomic shock in Chlamydomonas triploids.","authors":"Lucas Prost-Boxoen, Quinten Bafort, Antoine Van de Vloet, Fabricio Almeida-Silva, Yunn Thet Paing, Griet Casteleyn, Sofie D'hondt, Olivier De Clerck, Yves Van de Peer","doi":"10.1111/nph.20249","DOIUrl":"https://doi.org/10.1111/nph.20249","url":null,"abstract":"Genome merging is a common phenomenon causing a wide range of consequences on phenotype, adaptation, and gene expression, yet its broader implications are not well-understood. Two consequences of genome merging on gene expression remain particularly poorly understood: dosage effects and evolution of expression. We employed Chlamydomonas reinhardtii as a model to investigate the effects of asymmetric genome merging by crossing a diploid with a haploid strain to create a novel triploid line. Five independent clonal lineages derived from this triploid line were evolved for 425 asexual generations in a laboratory natural selection experiment. Utilizing fitness assays, flow cytometry, and RNA-Seq, we assessed the immediate consequences of genome merging and subsequent evolution. Our findings reveal substantial alterations in genome size, gene expression, protein homeostasis, and cytonuclear stoichiometry. Gene expression exhibited expression-level dominance and transgressivity (i.e. expression level higher or lower than either parent). Ongoing expression-level dominance and a pattern of 'functional dominance' from the haploid parent was observed. Despite major genomic and nucleo-cytoplasmic disruptions, enhanced fitness was detected in the triploid strain. By comparing gene expression across generations, our results indicate that proteostasis restoration is a critical component of rapid adaptation following genome merging in Chlamydomonas reinhardtii and possibly other systems.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":null,"pages":null},"PeriodicalIF":9.4,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantifying past forest cover and biomass changes in the Ecuadorian Amazon. 量化厄瓜多尔亚马逊地区过去的森林覆盖率和生物量变化。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-11-05 DOI: 10.1111/nph.20237

Nina H Witteveen, Zoë S Kleijwegt, Hana Geara, Cathelijne Kool, Ansis Blaus, Lina Cabrera Saenz, Bianca Tacoronte Gomes, Annemarie Philip, Mark B Bush, Crystal N H McMichael

{"title":"Quantifying past forest cover and biomass changes in the Ecuadorian Amazon.","authors":"Nina H Witteveen, Zoë S Kleijwegt, Hana Geara, Cathelijne Kool, Ansis Blaus, Lina Cabrera Saenz, Bianca Tacoronte Gomes, Annemarie Philip, Mark B Bush, Crystal N H McMichael","doi":"10.1111/nph.20237","DOIUrl":"https://doi.org/10.1111/nph.20237","url":null,"abstract":"Here, we developed and applied models to quantitatively reconstruct forest cover and biomass changes at three lakes in northwestern Amazonia over the past > 1500 yr. We used remotely sensed data and a modern dataset of 50 Amazonian lakes to develop generalized linear models that predict aboveground biomass, using phytolith morphotypes and forest cover as predictor variables. Also, we applied a published beta regression model to predict forest cover within 200 m of each lake, using Poaceae phytoliths. Charcoal and maize phytoliths were analysed to identify past land use. Results showed forest cover and biomass changes at our study sites ranged between 48-84% and 142-438 Mg ha-1, respectively. Human occupation was discontinuous, with major changes in forest cover and biomass coinciding with periods of land use. Forest cover and biomass decreased notably after fire (at all sites) or cultivation events (Lakes Zancudococha, Kumpaka). The timing and ecological impact of past land use were spatially and temporally variable. Our results suggest past human impact was small-scaled and heterogenous in northwestern Amazonia, with a significant impact of fire on forest cover and biomass changes.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":null,"pages":null},"PeriodicalIF":9.4,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0