New Phytologist最新文献_第3页

Ten rules for robust taxonomy in ecological datasets. 生态数据集稳健性分类的十个规则。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-10-12 DOI: 10.1111/nph.71658

Renato A Ferreira de Lima, Guilherme S Grittz, Moabe F Fernandes, Hans Ter Steege, Kyle G Dexter, Oliver Phillips, Marli P Morim, Jérôme Chave, Géraldine Derroire, Filipe M França, Tamara Heartsill-Scalley, Ricardo A Segovia, Caroline E R Lehmann, Luis Cayuela, Karina Banda, Cecilia Blundo, Jesús Aguirre-Gutiérrez, André L de Gasper, R Toby Pennington

{"title":"Ten rules for robust taxonomy in ecological datasets.","authors":"Renato A Ferreira de Lima, Guilherme S Grittz, Moabe F Fernandes, Hans Ter Steege, Kyle G Dexter, Oliver Phillips, Marli P Morim, Jérôme Chave, Géraldine Derroire, Filipe M França, Tamara Heartsill-Scalley, Ricardo A Segovia, Caroline E R Lehmann, Luis Cayuela, Karina Banda, Cecilia Blundo, Jesús Aguirre-Gutiérrez, André L de Gasper, R Toby Pennington","doi":"10.1111/nph.71658","DOIUrl":"https://doi.org/10.1111/nph.71658","url":null,"abstract":"Accurate taxonomy, including species identification and naming, underpins ecological inference, yet taxonomic information in ecological datasets is often inconsistently recorded or weakly standardized. With the increasing integration of ecological datasets across taxa and regions, datasets with poor taxonomic practices can hinder data exchange and propagate errors. We propose 10 rules to improve the storage and management of taxonomic information in ecological datasets. These rules draw on our experiences in plant ecology and taxonomy, but the conclusions may be generalized to other organisms and disciplines. They emphasize aspects from species identification to the permanent storage of verifiable records (e.g. voucher specimens) and include taxonomic standardization, nomenclature, validation, and updating. We also address the treatment of morphotypes and incomplete identifications, the use of automated name-matching tools, and (meta)data standards. Closer collaboration between ecologists and taxonomists is key to ensuring more robust datasets. The application of these rules consumes more time and resources, but they enhance data quality and interoperability, saving time during data integration and improving the reliability and scalability of ecological research.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":8.1,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149171787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Avirulence genes of Magnaporthe oryzae are a pathogen defense arsenal shaped by cognate rice resistance genes in the field. 稻瘟病菌的无毒基因是由同源水稻抗性基因在田间形成的病原防御库。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-10-12 DOI: 10.1111/nph.71586

Xueyan Wang, Grant T Billings, Amanda M Hulse-Kemp, Quentin D Read, Yulin Jia, Melissa H Jia, Yixiao Huang, Yeshi Wamishe, Barbara Valent

{"title":"Avirulence genes of Magnaporthe oryzae are a pathogen defense arsenal shaped by cognate rice resistance genes in the field.","authors":"Xueyan Wang, Grant T Billings, Amanda M Hulse-Kemp, Quentin D Read, Yulin Jia, Melissa H Jia, Yixiao Huang, Yeshi Wamishe, Barbara Valent","doi":"10.1111/nph.71586","DOIUrl":"https://doi.org/10.1111/nph.71586","url":null,"abstract":"We examined the frequency of and variation in eight avirulence (AVR) genes from 840 Magnaporthe oryzae field isolates collected from the Southern United States relative to rice resistance (R) genes deployed from 1959 to 2017. Little coding or noncoding variation was detected in AVR-Pi9, AVR-Pib, ACE1 and AVR-Pizt, which correspond to R genes not used in the United States. In the United States, AVR-Pik, corresponding to rice R gene Pi-k alleles deployed at varying levels since the late 1960s, was present in 37% of the isolates over six decades. AVR-Pita1, corresponding to Pi-ta deployed in the late 1990s, reached a low point in the 5-yr period following Pi-ta deployment and then recovered to 99% in recent years. Our results suggest a fitness penalty for M. oryzae isolates carrying nonfunctional AVR variants and provide concrete data on how changes in plant R gene composition can impact pathogen populations. These findings will inform localized strategies for R gene usage and durable control of blast disease. AVR frequencies differ over time between United States and global samples for AVR-Pik, AVR-Pi9, AVR-Pii and AVR-Pita1. Further whole genome analysis including additional US samples is necessary to confirm whether the United States is a geographically isolated population.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":8.1,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149168674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Jasmonate biosynthesis regulates key events in barley vegetative and reproductive development. 茉莉酸盐生物合成调节大麦营养和生殖发育的关键事件。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-10-07 DOI: 10.1111/nph.71566

Jian Luo, Linsan Liu, Xiujuan Yang, Olivia Doolan, Tanicha Duangta, Emily Lyon, Jonathan Griffin, Jennifer Shoesmith, Wenhao Wu, Ruth Hamilton, Mirjam Nuter, Mikah Katay-Fodor, Neil Bricklebank, Nicola Aberdein, Guoping Zhang, Colin Turnbull, Matthew R Tucker, Sarah M McKim

{"title":"Jasmonate biosynthesis regulates key events in barley vegetative and reproductive development.","authors":"Jian Luo, Linsan Liu, Xiujuan Yang, Olivia Doolan, Tanicha Duangta, Emily Lyon, Jonathan Griffin, Jennifer Shoesmith, Wenhao Wu, Ruth Hamilton, Mirjam Nuter, Mikah Katay-Fodor, Neil Bricklebank, Nicola Aberdein, Guoping Zhang, Colin Turnbull, Matthew R Tucker, Sarah M McKim","doi":"10.1111/nph.71566","DOIUrl":"https://doi.org/10.1111/nph.71566","url":null,"abstract":"Cereal grain is a global food staple. Filial tissues in grain, such as the embryo and endosperm, grow embedded in the maternal ovule and surrounding ovary wall. Maternal tissues provide nutrition and protection but are progressively reduced and degraded as filial tissues expand, driving grain expansion between floret hulls. While we know some enzymatic players, regulation of this process remains less understood. However, our previous work in barley, Hordeum vulgare spp. vulgare suggested a role for the phytohormone jasmonate (JA). We edited the barley ALLENE OXIDASE CYCLASE (HvAOC) gene encoding a JA biosynthetic enzyme. We examined hvaoc mutants for developmental phenotypes, genetic interactions and effects on the transcriptome. Defective HvAOC alleles reduced JA levels, and caused enhanced vegetative growth, faster flowering and lengthened grain, all phenotypes rescued by MeJA treatment. HvAOC control of grain length appears parentally-derived, consistent with enriched HvAOC expression in maternal compared to filial tissues. Grain dimension changes were uncoupled from hull size and correlated with larger endosperm cavities, more persistent maternal tissues and higher expression of genes linked to sucrose transport. This work provides the first genetic dissection of JA in barley and highlights roles in coordinating maternal tissues within grain.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":8.1,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149128854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ENDOSOMAL SORTING COMPLEX REQUIRED FOR TRANSPORT machineries play crucial roles in vacuolar trafficking and cytokinesis in the moss Physcomitrium patens. 运输机械所需的内体分选复合物在苔藓液泡运输和细胞分裂中起着至关重要的作用。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-10-07 DOI: 10.1111/nph.71651

Jingtong Ruan, Zihan Yin, Yirong Gan, Lili Jiang, Caiwan Guo, Peishan Yi

{"title":"ENDOSOMAL SORTING COMPLEX REQUIRED FOR TRANSPORT machineries play crucial roles in vacuolar trafficking and cytokinesis in the moss Physcomitrium patens.","authors":"Jingtong Ruan, Zihan Yin, Yirong Gan, Lili Jiang, Caiwan Guo, Peishan Yi","doi":"10.1111/nph.71651","DOIUrl":"https://doi.org/10.1111/nph.71651","url":null,"abstract":"The ENDOSOMAL SORTING COMPLEX REQUIRED FOR TRANSPORT (ESCRT) proteins are essential for converting early endosomes (EEs) into late endosomes/multivesicular bodies (LEs/MVBs) during endocytosis. In animals, they also play noncanonical roles in cytokinetic abscission, membrane repair, and other membrane remodeling processes. However, their functions in plants, especially nonvascular plants, remain poorly understood. Here, we systematically investigate the localization and functions of ESCRT proteins in the moss Physcomitrium patens using fluorescent protein labeling, mutant analyses, interaction assays, and electron microscopy. We show that ESCRT-related proteins localize to endosomes and play a conserved role in endocytic trafficking. Notably, all tested ESCRT components also localize to the developing cell plate, whereas reporters involved in trans-Golgi network (TGN)-MVB transition do not. Disruption of VPS4, a key regulator of ESCRT-III, delays cytokinesis progression and alters the dynamics of cell plate assembly factors. We propose that the ESCRT machinery plays both conserved and previously unrecognized noncanonical roles in plants. By providing this evidence in a bryophyte, our work establishes a critical reference point for future functional studies in nonvascular plants and for evolutionary comparisons across plant lineages.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":8.1,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149128919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distinct targeting of 5mC and 6mA DNA methylation during the onset of Flg22-induced immunity between Arabidopsis and rice. flg22诱导的拟南芥和水稻免疫启动过程中5mC和6mA DNA甲基化的不同靶向性

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-10-07 DOI: 10.1111/nph.71600

Jasper Matthys, Ibe Naessens, Filip Van Nieuwerburgh, Jurriaan Ton, Tina Kyndt

{"title":"Distinct targeting of 5mC and 6mA DNA methylation during the onset of Flg22-induced immunity between Arabidopsis and rice.","authors":"Jasper Matthys, Ibe Naessens, Filip Van Nieuwerburgh, Jurriaan Ton, Tina Kyndt","doi":"10.1111/nph.71600","DOIUrl":"https://doi.org/10.1111/nph.71600","url":null,"abstract":"Cytosine methylation drives transcriptional reprogramming during plant innate and acquired immunity. Beyond RNA adenosine methylation, DNA adenine methylation (6mA) has recently emerged as a candidate regulator of gene expression, though its role in plant immunity is unknown. Using the bacterial elicitor Flg22, we examined genome-wide DNA methylation responses of Arabidopsis thaliana and Oryza sativa to identify shared epigenetic responses across divergent genomes. High-resolution Oxford Nanopore sequencing enabled simultaneous detection of 5-methylcytosine (5mC) and 6mA DNA modifications. In both species, early immune activation was marked by non-CG 5mC hypomethylation at short euchromatic transposable elements. By 72 hpt, both species showed elevated 5mC levels at (peri)centromeric regions, targeted to pericentromeric non-CG sites in Arabidopsis and accompanied by lasting chromosomal arm hypomethylation in rice. Despite species-specific global 6mA responses, both species exhibited a conserved bimodal 6mA distribution flanking transcription start and termination sites of genes, intensified at defence-related genes following Flg22 elicitation. In Arabidopsis, these 6mA signatures correlated with Flg22-primed gene activation, while mutation of 6mA demethylase ALKBH1D increased susceptibility to Pseudomonas syringae and Meloidogyne incognita. Together, our findings reveal conserved 5mC dynamics during immune activation and highlight a role for 6mA in modulating defence-related transcriptional reprogramming.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":8.1,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149128902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

STRONG3 promotes lignin biosynthesis and culm strength in rice. STRONG3促进水稻木质素的生物合成和茎秆强度。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-10-07 DOI: 10.1111/nph.71574

Yong Zhao, Jie Gao, Shichen Han, Ran Xu, Bingxia Xu, Muhammad Abdul Rehman Rashid, Xingming Sun, Jinjie Li, Hongliang Zhang, Lujia Han, Wensheng Wang, Zichao Li, Zhanying Zhang

{"title":"STRONG3 promotes lignin biosynthesis and culm strength in rice.","authors":"Yong Zhao, Jie Gao, Shichen Han, Ran Xu, Bingxia Xu, Muhammad Abdul Rehman Rashid, Xingming Sun, Jinjie Li, Hongliang Zhang, Lujia Han, Wensheng Wang, Zichao Li, Zhanying Zhang","doi":"10.1111/nph.71574","DOIUrl":"https://doi.org/10.1111/nph.71574","url":null,"abstract":"Lodging severely reduces rice yield and quality. Culm strength, mainly determined by lignin biosynthesis and secondary cell wall (SCW) formation, is core to lodging resistance. We identified an ethylene response factor (ERF) transcription factor STRONG3 via genome-wide association study (GWAS) of 405 rice accessions based on lignin content. Functional validation demonstrated that STRONG3 positively regulates lignin accumulation, culm diameter, and mechanical strength by promoting sclerenchyma tissue development, vascular bundle enlargement, and SCW thickening. Molecular analyses revealed that STRONG3 directly binds to the GCC-box motif in the promoter of 4CL4, a rate-limiting enzyme in the phenylpropanoid pathway, to activate its transcription. Genetic complementation confirmed that 4CL4 acts downstream of STRONG3 to mediate lignin biosynthesis and culm development. Haplotype analysis identified subspecies-specific favorable alleles: STRONG3G in Geng rice, caused by a single nucleotide polymorphism (4349512) leading to an Ala289Thr substitution in the activation domain that enhances STRONG3's transcriptional activation of 4CL4, and 4CL4TC in Xian rice. Combinatorial analysis showed these alleles synergistically improve culm traits in their respective subspecies. Overexpression of STRONG3 enhanced lodging resistance without compromising grain yield. Our findings uncover a critical regulatory pathway for rice culm development and provide valuable genetic resources and molecular markers for breeding high-yield, lodging-resistant rice varieties.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":8.1,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149128873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dodder seedlings benefit from apoplastic nutrition by inducing SWEET15-mediated unloading of sucrose from the host's phloem. 菟丝子幼苗通过诱导sweet15介导的蔗糖从宿主韧皮部卸载而受益于外质体营养。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-10-01 Epub Date: 2026-05-16 DOI: 10.1111/nph.71270

Maleen Hartenstein, Dominik Eydam, Antonia Klatt, Arne Greif, Peter Slaby, Ronja Burggraf, Franz Klebl, Isabell Albert, Uwe Sonnewald, Ruth Stadler, Markus Albert

{"title":"Dodder seedlings benefit from apoplastic nutrition by inducing SWEET15-mediated unloading of sucrose from the host's phloem.","authors":"Maleen Hartenstein, Dominik Eydam, Antonia Klatt, Arne Greif, Peter Slaby, Ronja Burggraf, Franz Klebl, Isabell Albert, Uwe Sonnewald, Ruth Stadler, Markus Albert","doi":"10.1111/nph.71270","DOIUrl":"10.1111/nph.71270","url":null,"abstract":"Cuscuta species are holoparasitic plants that penetrate host stems with haustoria, connect to the vasculature and exhaust water, nutrients and carbohydrates. Parasite seedlings possess only limited maternal reserves and the development of a vascular connection takes several days. Until then, an alternative access to host assimilates is critical for the survival of the parasite. Here, we investigated the sucrose flux and nutrition mechanisms of dodder during early infection. Using phloem-mobile fluorescent tracers, we show that an apoplastic sucrose transfer occurs before symplastic connections between host and parasite are formed. We identified sucrose transporters of both dodder and host that are critical for assimilate transfers. CrSUT1, CrSUT3 and CrSWEET10 mediate sucrose transport during haustorium development. In the phloem of Arabidopsis, at the infection site, the sucrose-exporter AtSWEET15 is induced through basic leucine zipper transcription factors. CrSUT2 was identified as a transporter for sucrose uptake into the invading haustorium. The growth of dodder seedlings on atsweet15 hosts is reduced, demonstrating the importance of the sucrose export by AtSWEET15 and availability within the apoplast. Our data highlight the relevance of apoplastic nutrition for the survival of parasitic plants. Dodder manipulates the host, causing it to release carbohydrates from its phloem during the initial infection.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"829-842"},"PeriodicalIF":8.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13583392/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147949716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mul-PheG2P: decoupled learning and prediction-space fusion enables robust and interpretable multi-phenotype genomic prediction. multi- pheg2p：解耦学习和预测空间融合实现稳健和可解释的多表型基因组预测。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-10-01 Epub Date: 2026-08-04 DOI: 10.1111/nph.71461

Jiahui Wang, Yong Zhang, Bo Li, Xinglin Piao, Xiangyu Zhao, Dongfeng Zhang, Aiwen Wang, Bob Zhang, Kaiyi Wang

{"title":"Mul-PheG2P: decoupled learning and prediction-space fusion enables robust and interpretable multi-phenotype genomic prediction.","authors":"Jiahui Wang, Yong Zhang, Bo Li, Xinglin Piao, Xiangyu Zhao, Dongfeng Zhang, Aiwen Wang, Bob Zhang, Kaiyi Wang","doi":"10.1111/nph.71461","DOIUrl":"10.1111/nph.71461","url":null,"abstract":"Genomic prediction of multiple phenotypes is crucial in modern plant breeding; however, existing methods struggle with negative transfer and lack interpretability, particularly across high-dimensional small-sample data and diverse species. To address this, we propose Mul-PheG2P, a novel paradigm based on decoupled learning and predictive space fusion. It employs a two-stage design: first training phenotype-specific encoders using genetic data, then decoupling phenotype-specific learning from cross-phenotype aggregation via an interpretable prediction layer. Mul-PheG2P outperforms existing methods across diverse crop datasets, including maize (Zea mays), wheat (Triticum aestivum), and tomato (Solanum lycopersicum). It provides a multi-scale interpretability chain: at the macro level, it quantifies phenotypic contributions via attention-based weighting; at the micro level, Integrated Gradients reveal the genetic basis of predictions. Notably, the model successfully identified the CCT (CONSTANS, CO-like, and TOC) motif regulating photoperiodism and the SQUAMOSA (SQUAMOSA promoter binding protein) promoter for inflorescence development, confirming its ability to capture functional biological mechanisms. These results highlight the high performance and interpretability of Mul-PheG2P, showcasing its value for low-cost, large-scale screening to advance precision breeding.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"503-519"},"PeriodicalIF":8.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13539974/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148674549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Domestication-associated reduction of methyl salicylate in tomato root and its significance for resistance to root-knot nematode. 驯化相关的番茄根系水杨酸甲酯含量降低及其对根结线虫抗性的意义

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-10-01 Epub Date: 2026-07-29 DOI: 10.1111/nph.71478

Weijiao Wang, Manoj Sapkota, Sobhan Bahrami Zadegan, Tarek Hewezi, Esther van der Knaap, Feng Chen

{"title":"Domestication-associated reduction of methyl salicylate in tomato root and its significance for resistance to root-knot nematode.","authors":"Weijiao Wang, Manoj Sapkota, Sobhan Bahrami Zadegan, Tarek Hewezi, Esther van der Knaap, Feng Chen","doi":"10.1111/nph.71478","DOIUrl":"10.1111/nph.71478","url":null,"abstract":"Methyl salicylate (MeSA) plays diverse roles in the aerial parts of plants. By contrast, its biosynthesis and function in roots remain poorly understood. Here, we investigated root MeSA biosynthesis and function in tomato. Genome-wide association studies (GWAS) were performed using root MeSA levels as the phenotype in a diversity panel of 167 accessions to identify associated loci. Candidate genes were biochemically characterized, and the role of MeSA in defense against root-knot nematode (RKN, Meloidogyne incognita) was evaluated using transgenic plants. MeSA was identified as a major root volatile in tomato and showed a domestication-associated reduction. GWAS revealed multiple loci associated with natural variation in root MeSA, including a major locus on Chromosome 9 encoding the salicylic acid methyltransferase (SlSAMT). SlSAMT-overexpressing plants showed reduced resistance to RKNs, whereas SlSAMT-knockdown plants exhibited enhanced resistance. Our results suggest complex roles of MeSA and the salicylic acid (SA) signaling pathway in belowground plant defense. The SA signaling pathway likely plays critical roles in protecting roots against diverse natural enemies, including RKNs. Nevertheless, RKNs appear to have co-opted MeSA as a host-location signal, and the domestication-associated reduction of root MeSA in tomato has likely contributed to enhanced resistance against RKNs.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"414-426"},"PeriodicalIF":8.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13539948/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148622240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New components in genetic regulation of age-dependent compound leaf architecture in pea. 豌豆年龄依赖性复叶结构遗传调控中的新成分。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-10-01 Epub Date: 2026-08-14 DOI: 10.1111/nph.71515

Jacqueline K Vander Schoor, Chantelle J Beagley, Warwick Gill, Grégoire Aubert, Judith Burstin, Robert J E Wiltshire, Valérie Hecht, James L Weller

{"title":"New components in genetic regulation of age-dependent compound leaf architecture in pea.","authors":"Jacqueline K Vander Schoor, Chantelle J Beagley, Warwick Gill, Grégoire Aubert, Judith Burstin, Robert J E Wiltshire, Valérie Hecht, James L Weller","doi":"10.1111/nph.71515","DOIUrl":"10.1111/nph.71515","url":null,"abstract":"Pea (Pisum sativum L.) is a classic model for compound leaf development. Pea leaves typically consist of a proximal series of paired lateral leaflets substituted distally by filiform tendrils, and the number of lateral organ pairs increases with ontogeny. This gradient has been proposed as an expression of heteroblasty, but no clear links with known leaf-development pathways have been demonstrated to date. We explored potential links between lateral organ number, heteroblasty and flowering time in pea using natural and induced genetic variation, and characterize four distinct loci regulating compound leaf architecture through genetic and molecular analyses. Two previously described AEROMACULATA (AERO) loci AERO1 and AERO2 are identified as putative orthologs of Arabidopsis chromatin modifiers CURLY LEAF and PICKLE, and two novel loci EXTRA PINNA PAIRS 1 (EPP1) and EPP2 as co-orthologs of the transcriptional adaptor SEUSS. These loci interact to influence a range of pleiotropic phenotypes related to meristem function. AERO and EPP genes controlling lateral organ number in pea leaves have no clear association with leaf complexity or heteroblasty in other systems, and represent new components in regulation of legume compound leaf development.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"909-925"},"PeriodicalIF":8.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13583374/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148765418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0