New Phytologist最新文献_第4页

Geology, climate, and competition as drivers of diversification in the neotropical palm genus Geonoma. 地质、气候和竞争是新热带棕榈属植物多样化的驱动因素。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-10-01 Epub Date: 2026-07-29 DOI: 10.1111/nph.71469

Yaël Assassa, Oriane Loiseau, Anna Weigand, Ingrid Olivares, María José Sanín, Natalia Arcila Marin, Margot Paris, Marylaure de La Harpe, Christophe Thébaud, Michael Kessler, Nicolas Salamin, Jonathan Rolland

{"title":"Geology, climate, and competition as drivers of diversification in the neotropical palm genus Geonoma.","authors":"Yaël Assassa, Oriane Loiseau, Anna Weigand, Ingrid Olivares, María José Sanín, Natalia Arcila Marin, Margot Paris, Marylaure de La Harpe, Christophe Thébaud, Michael Kessler, Nicolas Salamin, Jonathan Rolland","doi":"10.1111/nph.71469","DOIUrl":"10.1111/nph.71469","url":null,"abstract":"Understanding why the Neotropics are one of the most biodiverse regions on earth remains a major challenge in ecology and evolutionary biology. We still know little about how the complex interplay between the geological history, climatic fluctuations, and interspecific interactions has led to the build-up of present-day diversity patterns. Here, we explore patterns of diversification in the diverse palm genus Geonoma, encompassing nearly 70 species throughout Central and South America, using phylogenetic analyses, models of diversification and phenotypic evolution. The clade originated in the Andes and subsequently dispersed toward Amazonia and Central America. The speciation rate has been constantly declining through time, possibly related to temperature cooling or increased interspecific competition for resources. We also found an exceptionally high disparity for 28 traits suggesting potential niche partitioning and character displacement, a result partially supported by models of phenotypic evolution. Overall, our study unravels the influence of geological, abiotic, and biotic factors at different stages of the diversification process of Geonoma and illustrates how their complex interactions contribute to the buildup of neotropical diversity.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"443-458"},"PeriodicalIF":8.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13539966/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148622236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Turnip mosaic virus utilizes the lipid droplet biogenesis machinery to facilitate its propagation in plants. 芜菁花叶病毒利用脂滴生物发生机制促进其在植物体内的繁殖。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-10-01 Epub Date: 2026-06-09 DOI: 10.1111/nph.71321

Léna Jambou, Nathalie Arvy, Marguerite Batsale, Luc Sofer, Sara Shakir, Marielle Cochet, Vincent Simon, Charlotte Quinteau, Thomas Scotti, Nathan M Doner, Satinder K Gidda, Robert T Mullen, Sabine D'Andréa, Jean-Luc Gallois, Denis Coulon, Claire Bréhélin, Sylvie German-Retana

{"title":"Turnip mosaic virus utilizes the lipid droplet biogenesis machinery to facilitate its propagation in plants.","authors":"Léna Jambou, Nathalie Arvy, Marguerite Batsale, Luc Sofer, Sara Shakir, Marielle Cochet, Vincent Simon, Charlotte Quinteau, Thomas Scotti, Nathan M Doner, Satinder K Gidda, Robert T Mullen, Sabine D'Andréa, Jean-Luc Gallois, Denis Coulon, Claire Bréhélin, Sylvie German-Retana","doi":"10.1111/nph.71321","DOIUrl":"10.1111/nph.71321","url":null,"abstract":"Lipid droplets (LDs), which are dedicated to storing neutral lipids (NLs), are dynamic organelles involved in numerous other functions, including membrane remodeling during abiotic stress. While the hijacking of LDs by animal viruses is well documented, their role(s) in plant virus infection remains largely unexplored. Similar to animal viruses, plant potyviruses reroute host proteins, intracellular membranes, and lipids to create an optimized microenvironment for their efficient viral replication compartment (VRC) assembly and movement. We therefore investigated whether potyviruses can also exploit the LD biogenesis machinery for their own benefit, akin to some animal viruses. Utilizing lipid analyses, confocal and transmission electron microscopy, and viral propagation surveys in plants impaired in LD biogenesis, we show that potyvirus turnip mosaic virus (TuMV) infection induces a significant accumulation of NLs and a proliferation of LDs in Nicotiana benthamiana or Arabidopsis thaliana-infected leaves, which are often located in close proximity to VRCs. This suggests that there is a spatial and functional relationship between LDs and TuMV. We demonstrate also that two protein families crucial for LD biogenesis, namely the lipid droplet-associated proteins and SEIPINs, facilitate TuMV propagation in Arabidopsis thaliana. Taken together, our results indicate a pro-viral function for LDs in potyvirus-infected plants.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"797-816"},"PeriodicalIF":8.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13583387/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148212682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A cooperative regulatory module between TAGL2 and JMJC1 activates specific defense genes against root-knot nematodes in tomato. TAGL2和JMJC1之间的协同调控模块激活了番茄根结线虫的特异性防御基因。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-10-01 Epub Date: 2026-08-09 DOI: 10.1111/nph.71496

Ting Yang, Yile Miao, Jiameng Zhao, Weiduo Jin, Tingting Ran, Tina Kyndt, Jingquan Yu, Jie Zhou

{"title":"A cooperative regulatory module between TAGL2 and JMJC1 activates specific defense genes against root-knot nematodes in tomato.","authors":"Ting Yang, Yile Miao, Jiameng Zhao, Weiduo Jin, Tingting Ran, Tina Kyndt, Jingquan Yu, Jie Zhou","doi":"10.1111/nph.71496","DOIUrl":"10.1111/nph.71496","url":null,"abstract":"Plant-parasitic nematodes (PPNs) threaten global food security. Although epigenetic modifications are crucial for plant immunity, how histone modifiers contribute to root-knot nematodes (RKNs, Meloidogyne incognita) resistance remains unclear. Here, using genetic, molecular and biochemical approaches, we investigated the epigenetic and transcriptional mechanisms underlying RKN resistance mediated by the histone demethylase (HDM) JMJC1 and the MADS-box transcription factor TAGL2 in tomato (Solanum lycopersicum). We identified JMJC1 as an RKN-induced positive defense regulator targeting H3K9me3 and H3K27me3 histone marks. JMJC1 physically interacts with TAGL2, which also positively regulates RKN resistance. Transcriptomic analysis indicated that TAGL2 regulates multiple layers of the plant defense network, transcriptionally activating representative genes from distinct pathways (including PUB10, bHLH98, CCaMK, and SAUR3), which we validated as positive regulators of RKN resistance via virus-induced gene silencing (VIGS). At the chromatin level, TAGL2 and JMJC1 co-regulate these loci, associating with localized H3K9me3 and H3K27me3 reduction. Furthermore, TAGL2 directly activates JMJC1 transcription, establishing a positive feedback loop that amplifies immune signaling. Our findings reveal a cooperative model wherein a HDM and a transcription factor coordinate at specific loci to fine-tune multiple defense layers at both epigenetic and transcriptional levels, providing insights for breeding durable nematode-resistant plants.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"340-358"},"PeriodicalIF":8.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13539946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148701942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ZmHPATR1 orchestrates phyllosphere organic acid flux to recruit Sphingomonas and enhance resistance of maize to Curvularia leaf spot. ZmHPATR1调控叶层有机酸通量招募鞘氨单胞菌，增强玉米对曲霉叶斑病的抗性。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-10-01 Epub Date: 2026-08-03 DOI: 10.1111/nph.71485

Xinhao Luo, Hanchen Shan, Boyan Wang, Lulu Qi, Mingbi Ding, Yongxia Qi, Jing Zhou, Jun Fan, Guomin Han, Beijiu Cheng, Jin Chen, Xiaoyu Li

{"title":"ZmHPATR1 orchestrates phyllosphere organic acid flux to recruit Sphingomonas and enhance resistance of maize to Curvularia leaf spot.","authors":"Xinhao Luo, Hanchen Shan, Boyan Wang, Lulu Qi, Mingbi Ding, Yongxia Qi, Jing Zhou, Jun Fan, Guomin Han, Beijiu Cheng, Jin Chen, Xiaoyu Li","doi":"10.1111/nph.71485","DOIUrl":"10.1111/nph.71485","url":null,"abstract":"Leaf spots caused by Curvularia lunata infection pose a significant threat to global maize production. Although resistance gene breeding faces challenges due to pathogen evolution, the plant microbiome has emerged as a key modulator of disease resistance. However, the mechanisms via which plant genes regulate phyllosphere metabolites to recruit beneficial microbes remain poorly understood. Here, we combined gene mapping, metabolomics, microbiome analyses, cytological analysis, and in vitro and in vivo experiments to investigate the disease resistance mechanism of ZmHPATR1. We first identified that the loss-of-function mutation in ZmHPATR1 significantly increased the levels of fumaric acid, folic acid, and tetrahydrofolic acid in the leaves, leading to the enrichment of the genus Sphingomonas. We further demonstrated that the extracellular polysaccharide, welan gum, biosynthesized by Sphingomonas, effectively inhibited C. lunata growth and disrupted its cell structure. These results enable us to comprehensively understand the complicated mechanisms of plant resistance to disease through a four-level regulatory network that links plant genes, metabolites, microbes, and pathogens. Our findings provide new strategies for targeted microbiome-based disease-resistant breeding and the development of novel biopesticides for maize.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"359-374"},"PeriodicalIF":8.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13539943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148671117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of an efficient and PAM-relaxed LbCas12a genome editing tool in plants. 一种高效且pam松弛的植物LbCas12a基因组编辑工具的建立

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-10-01 Epub Date: 2026-08-04 DOI: 10.1111/nph.71498

Yaolong Wang, Huanhuan Xu, Wenjun Lu, Zhiwen Wan, Ziyan Xu, Wenlong Wang, Chengyu Chen, Eryang Pan, Fangling Jiang, Tongkun Liu, Ying Li, Dong Xiao, Xuedong Yang, Fangfang Li, Xilin Hou, Changwei Zhang

{"title":"Establishment of an efficient and PAM-relaxed LbCas12a genome editing tool in plants.","authors":"Yaolong Wang, Huanhuan Xu, Wenjun Lu, Zhiwen Wan, Ziyan Xu, Wenlong Wang, Chengyu Chen, Eryang Pan, Fangling Jiang, Tongkun Liu, Ying Li, Dong Xiao, Xuedong Yang, Fangfang Li, Xilin Hou, Changwei Zhang","doi":"10.1111/nph.71498","DOIUrl":"10.1111/nph.71498","url":null,"abstract":"Cas12a is widely used in plant genome editing, but its targeting scope is constrained by stringent protospacer adjacent motif (PAM) requirements and variable activity across species, limiting its application at diverse genomic loci. LbCas12a-RRV-based editing system was established in nonheading Chinese cabbage, and T5exo-PF-LbCas12a was generated by introducing a triple mutation (D535G/S551F/D665N) and fusing with T5 exonuclease. This engineered system recognizes an expanded PAM sequence from 5'-VTTV-3' to 5'-NYHV-3'. The system exhibited efficient editing at noncanonical PAM sites in cabbage, tomato, and rice. Additionally, it successfully mediated large-fragment deletions via microhomology-mediated end joining (MMEJ) in plants. This study expands Cas12a targeting scope in plants and provides the first evidence for Cas12-mediated MMEJ-based large-fragment deletion. The toolkit facilitates functional genomics and crop improvement, and the methodology is readily adaptable to other plant species.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"936-949"},"PeriodicalIF":8.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13583368/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148674523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intraspecific trait variation mediates realized extinction and unpaid extinction debt in island ecosystems. 种内性状变异介导了岛屿生态系统中已实现的灭绝和未支付的灭绝债务。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-10-01 Epub Date: 2026-07-24 DOI: 10.1111/nph.71467

Aiying Zhang, Shiting Zhang, Yijuan Song, Mingjian Yu, Lingfeng Mao

{"title":"Intraspecific trait variation mediates realized extinction and unpaid extinction debt in island ecosystems.","authors":"Aiying Zhang, Shiting Zhang, Yijuan Song, Mingjian Yu, Lingfeng Mao","doi":"10.1111/nph.71467","DOIUrl":"10.1111/nph.71467","url":null,"abstract":"The Equilibrium Theory of Island Biogeography (ETIB) explains species diversity through colonization-extinction balance yet assumes species are functionally uniform. We extend ETIB by incorporating intraspecific trait variation (ITV) and species abundance into extinction dynamics in 66 woody plant species on 29 land-bridge islands in China's Thousand Island Lake. Using demographic censuses from 2009 to 2019 and five traits measured in 3990 individuals, we quantified species-level mortality, realized extinction rate, and unpaid extinction debt. Generalized linear mixed models and double-response piecewise structural equation models evaluated spatial, demographic, and trait-mediated pathways. Species abundance and island area dominate species-level mortality. Community-level ITV in leaf area and leaf thickness reduced realized extinction rate; ITV in specific leaf area reduced unpaid extinction debt, and ITV in leaf dry matter content amplified realized extinction rate. Extinction risk therefore depends not only on which species and traits occur on islands but also on how much coordinated functional variation is retained within species. ITV and community-weighted mean traits capture complementary components of extinction dynamics, extending island biogeography theory and clarifying biodiversity persistence in fragmented landscapes.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"475-487"},"PeriodicalIF":8.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13539960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148580841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FaNAC29: a novel gene positively regulating strawberry powdery mildew resistance. 一个正调控草莓白粉病抗性的新基因FaNAC29。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-10-01 Epub Date: 2026-08-25 DOI: 10.1111/nph.71517

Ruyu He, Lijuan Chen, Dong Wang, Qiao Xiao, Guowei Zhang, Jia Liu, Hongwen Li

{"title":"FaNAC29: a novel gene positively regulating strawberry powdery mildew resistance.","authors":"Ruyu He, Lijuan Chen, Dong Wang, Qiao Xiao, Guowei Zhang, Jia Liu, Hongwen Li","doi":"10.1111/nph.71517","DOIUrl":"10.1111/nph.71517","url":null,"abstract":"Powdery mildew (PM) of strawberry (Fragaria × ananassa) is a prevalent fungal disease affecting all aboveground tissues, especially leaves. Currently, there are few germplasm resources of strawberry resistant to PM, and the resistance mechanism remains unclear. This study constructed a BC1F1 segregating population by crossing the highly resistant 'Parajatan' with the susceptible 'Benihoppe'. Using multi-omics approaches, including RNA-seq, QTL-seq, and metabolomics analysis, a key gene FaNAC29 was identified. Subcellular localization revealed FaNAC29 nuclear expression, and transcriptional activation assays confirmed its activation activity. Notably, functional assays revealed that FaNAC29 confers enhanced PM resistance in strawberry seedlings. We further identified the downstream target gene FaPR4 and the interacting protein FaZHD11, using DAP-seq and Y2H assays, respectively. We characterized FaPR4 as a direct downstream target of FaNAC29, which enhances strawberry PM resistance via the modulation of chitinase activity. These findings provide a theoretical basis for breeding strawberry varieties with both excellent commercial traits and PM resistance.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"760-778"},"PeriodicalIF":8.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13583373/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148819946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phosphorylation of the transcription factor TgWRKY47 by TgSnRK1 modulates cone enlargement in the gymnosperm Torreya grandis. TgSnRK1磷酸化转录因子TgWRKY47调控裸子香榧圆锥体增大。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-10-01 Epub Date: 2026-08-28 DOI: 10.1111/nph.71525

Ya Liu, Ruoman Wang, Shuya Wang, Tongtong Wang, Jinwei Suo, Jingwei Yan, Jiasheng Wu

{"title":"Phosphorylation of the transcription factor TgWRKY47 by TgSnRK1 modulates cone enlargement in the gymnosperm Torreya grandis.","authors":"Ya Liu, Ruoman Wang, Shuya Wang, Tongtong Wang, Jinwei Suo, Jingwei Yan, Jiasheng Wu","doi":"10.1111/nph.71525","DOIUrl":"10.1111/nph.71525","url":null,"abstract":"Reproduction is essential for seed plant survival, and in gymnosperms, cone enlargement plays a pivotal role in seed production and reproduction. However, the molecular mechanisms regulating cone enlargement after fertilization remain poorly understood. Here, using Torreya grandis, a representative gymnosperm species with persistently low cone enlargement efficiency, we elucidated the molecular regulation of cone enlargement and identified sucrose non-fermenting 1-related protein kinase 1 (TgSnRK1) as a novel negative regulator of this process. TgSnRK1 physically interacted with the WRKY transcription factor TgWRKY47 both in vitro and in vivo. Functional analyses revealed that TgSnRK1 acted upstream of TgWRKY47 and suppressed cone enlargement in T. grandis. Phosphorylation assays revealed that TgSnRK1 directly phosphorylated TgWRKY47 at Thr-75. This phosphorylation event markedly reduced the transactivation activity of TgWRKY47, thereby attenuating its activation of the cell wall-remodeling gene TgEXPA2, a key regulator of cell expansion during cone enlargement. Together, these findings establish a phosphorylation-dependent TgSnRK1-TgWRKY47-TgEXPA2 regulatory pathway in which developmental attenuation of TgSnRK1-mediated phosphorylation of TgWRKY47 at Thr-75 enhances TgWRKY47 transactivation activity, thereby promoting TgEXPA2-dependent cone enlargement. This study advances our understanding of reproductive development in gymnosperms and identifies potential molecular targets for improving reproductive efficiency and seed yield.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"892-908"},"PeriodicalIF":8.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13583407/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148841760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sexual dimorphism in root exudation mediates belowground neighbor recognition and shapes rhizosphere soil microbial assembly. 根系分泌物中的两性二态现象介导地下邻居识别并形成根际土壤微生物群落。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-10-01 Epub Date: 2026-08-02 DOI: 10.1111/nph.71486

Qingxue Guo, Xinyi Chen, Jincheng Duan, Helena Korpelainen, Chunyang Li

{"title":"Sexual dimorphism in root exudation mediates belowground neighbor recognition and shapes rhizosphere soil microbial assembly.","authors":"Qingxue Guo, Xinyi Chen, Jincheng Duan, Helena Korpelainen, Chunyang Li","doi":"10.1111/nph.71486","DOIUrl":"10.1111/nph.71486","url":null,"abstract":"Sexual dimorphism in physiological traits is a hallmark of dioecious plants, yet whether these differences extend belowground to mediate neighbor recognition and rhizosphere microbiome assembly remains largely unexplored. Using split-root experiments, 13C-pulse labeling, and multi-omics with dioecious Populus cathayana, we dissected the interactions between plant sex, root exudation, and microbiome assembly. We demonstrated that male and female plants discriminate neighbor sex by modulating their root exudation. Females perceiving same-sex neighbors upregulated defense-related metabolites, whereas under inter-sexual interactions they shifted toward growth-promoting pathways. Males significantly increased the allocation of newly fixed photosynthetic carbon to the rhizosphere. Specifically, 13C incorporation into bacterial and fungal phospholipid fatty acids (PLFAs) was markedly higher when males grew in female-conditioned soil compared to male-conditioned soil. This male-driven carbon investment fostered highly interconnected cross-kingdom microbial networks with a significantly higher proportion of positive associations. Consistently, females grown under inter-sexual interactions exhibited greater nitrogen contents and higher photosynthetic rates than those under intra-sexual interactions. Our findings demonstrated that sexual dimorphism in root exudation drives the assembly of more complex and positively connected microbial networks, providing a transformative framework for understanding how belowground sexual recognition enhances the ecological resilience and productivity of mixed-sex plant populations.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"375-389"},"PeriodicalIF":8.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13539959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148664641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant genotypic diversity and defoliation jointly shape root exudation, fungal communities, and carbon and nitrogen cycling. 植物基因型多样性和落叶共同影响根系分泌物、真菌群落和碳氮循环。

IF 8.1 1区生物学

New Phytologist Pub Date : 2026-10-01 Epub Date: 2026-08-02 DOI: 10.1111/nph.71479

Irene Cordero, Mathilde Chomel, Ellen L Fry, Agnès Ardanuy, Ully Kritzler, Hayley Craig, Cristina Heredia-Acuña, Sarah Worthington, Stênio I A Foerster, Richard D Bardgett, David Johnson, Marina Semchenko

{"title":"Plant genotypic diversity and defoliation jointly shape root exudation, fungal communities, and carbon and nitrogen cycling.","authors":"Irene Cordero, Mathilde Chomel, Ellen L Fry, Agnès Ardanuy, Ully Kritzler, Hayley Craig, Cristina Heredia-Acuña, Sarah Worthington, Stênio I A Foerster, Richard D Bardgett, David Johnson, Marina Semchenko","doi":"10.1111/nph.71479","DOIUrl":"10.1111/nph.71479","url":null,"abstract":"Intraspecific plant diversity is a major component of community diversity, yet its role in shaping plant-microbial interactions and soil functioning remains poorly understood. In particular, plant genotypic diversity may modulate ecosystem responses to defoliation, a key driver of ecosystem processes in grazed systems. We subjected genotypic monocultures and mixtures of the grass Anthoxanthum odoratum to defoliation and quantified carbon fluxes, root exudation, and soil enzymatic activity. Using stable isotope labelling, we traced the movement of litter-derived 15N and photosynthetically derived 13C between plants and soil. The composition of soil fungal communities was assessed using ITS metabarcoding. Both defoliation and genotypic diversity increased root exudation, which was positively correlated with soil enzymatic activities and plant uptake of litter-derived 15N. The relative benefit of enhanced N mineralisation under defoliation depended on plant genotypic diversity: incorporation of 15N into leaves relative to 15N incorporation into soil microbial biomass was significantly higher in genotypic mixtures than in monocultures. Higher plant genotypic diversity was associated with lower soil fungal diversity, while defoliation increased saprotroph abundance. Plant genotypic diversity and defoliation jointly regulate plant-microbial interactions, with genotypic diversity enhancing plant performance under defoliation by shifting the partitioning of mineralised N from soil microbial biomass to plants.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":"324-339"},"PeriodicalIF":8.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13539967/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148664714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0