New Phytologist最新文献_第4页

Linking leaf dark respiration to leaf traits and reflectance spectroscopy across diverse forest types. 将不同森林类型的叶片暗呼吸与叶片特征和反射光谱学联系起来。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-11-19 DOI: 10.1111/nph.20267

Fengqi Wu, Shuwen Liu, Julien Lamour, Owen K Atkin, Nan Yang, Tingting Dong, Weiying Xu, Nicholas G Smith, Zhihui Wang, Han Wang, Yanjun Su, Xiaojuan Liu, Yue Shi, Aijun Xing, Guanhua Dai, Jinlong Dong, Nathan G Swenson, Jens Kattge, Peter B Reich, Shawn P Serbin, Alistair Rogers, Jin Wu, Zhengbing Yan

{"title":"Linking leaf dark respiration to leaf traits and reflectance spectroscopy across diverse forest types.","authors":"Fengqi Wu, Shuwen Liu, Julien Lamour, Owen K Atkin, Nan Yang, Tingting Dong, Weiying Xu, Nicholas G Smith, Zhihui Wang, Han Wang, Yanjun Su, Xiaojuan Liu, Yue Shi, Aijun Xing, Guanhua Dai, Jinlong Dong, Nathan G Swenson, Jens Kattge, Peter B Reich, Shawn P Serbin, Alistair Rogers, Jin Wu, Zhengbing Yan","doi":"10.1111/nph.20267","DOIUrl":"10.1111/nph.20267","url":null,"abstract":"Leaf dark respiration (Rdark), an important yet rarely quantified component of carbon cycling in forest ecosystems, is often simulated from leaf traits such as the maximum carboxylation capacity (Vcmax), leaf mass per area (LMA), nitrogen (N) and phosphorus (P) concentrations, in terrestrial biosphere models. However, the validity of these relationships across forest types remains to be thoroughly assessed. Here, we analyzed Rdark variability and its associations with Vcmax and other leaf traits across three temperate, subtropical and tropical forests in China, evaluating the effectiveness of leaf spectroscopy as a superior monitoring alternative. We found that leaf magnesium and calcium concentrations were more significant in explaining cross-site Rdark than commonly used traits like LMA, N and P concentrations, but univariate trait-Rdark relationships were always weak (r2 ≤ 0.15) and forest-specific. Although multivariate relationships of leaf traits improved the model performance, leaf spectroscopy outperformed trait-Rdark relationships, accurately predicted cross-site Rdark (r2 = 0.65) and pinpointed the factors contributing to Rdark variability. Our findings reveal a few novel traits with greater cross-site scalability regarding Rdark, challenging the use of empirical trait-Rdark relationships in process models and emphasize the potential of leaf spectroscopy as a promising alternative for estimating Rdark, which could ultimately improve process modeling of terrestrial plant respiration.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":9.4,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional characterization reveals the importance of Arabidopsis ECA4 and EPSIN3 in clathrin mediated endocytosis and wall structure in apical growing cells. 功能表征揭示了拟南芥 ECA4 和 EPSIN3 在凝集素介导的内吞和顶端生长细胞壁结构中的重要性。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-11-18 DOI: 10.1111/nph.20282

Rita Teresa Teixeira, Dario Marchese, Patrick J Duckney, Fernando Vaz Dias, Ana P Carapeto, Mariana Louro, Marta Sousa Silva, Carlos Cordeiro, Mário S Rodrigues, Rui Malhó

{"title":"Functional characterization reveals the importance of Arabidopsis ECA4 and EPSIN3 in clathrin mediated endocytosis and wall structure in apical growing cells.","authors":"Rita Teresa Teixeira, Dario Marchese, Patrick J Duckney, Fernando Vaz Dias, Ana P Carapeto, Mariana Louro, Marta Sousa Silva, Carlos Cordeiro, Mário S Rodrigues, Rui Malhó","doi":"10.1111/nph.20282","DOIUrl":"https://doi.org/10.1111/nph.20282","url":null,"abstract":"Localized clathrin mediated endocytosis is vital for secretion and wall deposition in apical growing plant cells. Adaptor and signalling proteins, along with phosphoinositides, are known to play a regulatory, yet poorly defined role in this process. Here we investigated the function of Arabidopsis ECA4 and EPSIN3, putative mediators of the process, in pollen tubes and root hairs. Homozygous eca4 and epsin3 plants exhibited altered pollen tube morphology (in vitro) and self-pollination led to fewer seeds and shorter siliques. These effects were augmented in eca4/epsin3 double mutant and quantitative polymerase chain reaction data revealed changes in phosphoinositide metabolism and flowering genes suggestive of a synergistic action. No visible changes were observed in root morphology, but atomic force microscopy in mutant root hairs showed altered structural stiffness. Imaging and FRET-FLIM analysis of ECA4 and EPSIN3 X-FP constructs revealed that both proteins interact at the plasma membrane but exhibit slightly different intracellular localization. FT-ICR-MS metabolomic analysis of mutant cells showed changes in lipids, amino acids and carbohydrate composition consistent with a role in secretion and growth. Characterization of double mutants of eca4 and epsin3 with phospholipase C genes (plc5, plc7) indicates that phosphoinositides (e.g. PtdIns(4,5)P2) are fundamental for a combined and complementary role of ECA4-EPSIN3 in cell secretion.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":9.4,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNA399s and strigolactones mediate systemic phosphate signaling between dodder-connected host plants and control association of host plants with rhizosphere microbes. MicroRNA399s 和绞股蓝内酯介导菟丝子寄主植物之间的系统磷酸盐信号传递，并控制寄主植物与根瘤微生物的结合。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-11-18 DOI: 10.1111/nph.20266

Man Zhao, Xijie Zheng, Zhongxiang Su, Guojing Shen, Yuxing Xu, Zerui Feng, Wenxing Li, Shuhan Zhang, Guoyan Cao, Jingxiong Zhang, Jianqiang Wu

{"title":"MicroRNA399s and strigolactones mediate systemic phosphate signaling between dodder-connected host plants and control association of host plants with rhizosphere microbes.","authors":"Man Zhao, Xijie Zheng, Zhongxiang Su, Guojing Shen, Yuxing Xu, Zerui Feng, Wenxing Li, Shuhan Zhang, Guoyan Cao, Jingxiong Zhang, Jianqiang Wu","doi":"10.1111/nph.20266","DOIUrl":"https://doi.org/10.1111/nph.20266","url":null,"abstract":"A dodder (Cuscuta) often simultaneously parasitizes two or more adjacent hosts. Phosphate (Pi) deficiency is a common stress for plants, and plants often interact with soil microbes, including arbuscular mycorrhizal fungi (AMF), to cope with Pi stress. Little is known about whether dodder transmits Pi deficiency-induced systemic signals between different hosts. In this study, dodder-connected plant clusters, each composed of two tobacco (Nicotiana tabacum) plants connected by a dodder, were established, and in each cluster, one of the two tobacco plants was treated with Pi starvation. AMF colonization efficiency, rhizosphere bacterial community, and transcriptome were analyzed in the other dodder-connected Pi-replete tobacco plant to study the functions of interplant Pi signals. We found that dodder transfers Pi starvation-induced systemic signals between host plants, resulting in enhanced AMF colonization, changes of rhizosphere bacterial communities, and alteration of transcriptomes in the roots of Pi-replete plants. Importantly, genetic analyses indicated that microRNA399s (miR399s) and strigolactones suppress the systemic Pi signals and negatively affect AMF colonization in the Pi-replete plants. These findings provide new insight into the ecological role of dodder in mediating host-host and host-microbe interactions and highlight the importance of strigolactone and miR399 pathways in systemic Pi signaling.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":9.4,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A fungal sRNA silences a host plant transcription factor to promote arbuscular mycorrhizal symbiosis. 真菌的 sRNA 能抑制宿主植物的转录因子，促进丛枝菌根共生。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-11-18 DOI: 10.1111/nph.20273

Alessandro Silvestri, William Conrad Ledford, Valentina Fiorilli, Cristina Votta, Alessia Scerna, Jacopo Tucconi, Antonio Mocchetti, Gianluca Grasso, Raffaella Balestrini, Hailing Jin, Ignacio Rubio-Somoza, Luisa Lanfranco

{"title":"A fungal sRNA silences a host plant transcription factor to promote arbuscular mycorrhizal symbiosis.","authors":"Alessandro Silvestri, William Conrad Ledford, Valentina Fiorilli, Cristina Votta, Alessia Scerna, Jacopo Tucconi, Antonio Mocchetti, Gianluca Grasso, Raffaella Balestrini, Hailing Jin, Ignacio Rubio-Somoza, Luisa Lanfranco","doi":"10.1111/nph.20273","DOIUrl":"https://doi.org/10.1111/nph.20273","url":null,"abstract":"Cross-kingdom RNA interference (ckRNAi) is a mechanism of interspecies communication where small RNAs (sRNAs) are transported from one organism to another; these sRNAs silence target genes in trans by loading into host AGO proteins. In this work, we investigated the occurrence of ckRNAi in Arbuscular Mycorrhizal Symbiosis (AMS). We used an in silico prediction analysis to identify a sRNA (Rir2216) from the AM fungus Rhizophagus irregularis and its putative plant gene target, the Medicago truncatula MtWRKY69 transcription factor. Heterologous co-expression assays in Nicotiana benthamiana, 5' RACE reactions and AGO1-immunoprecipitation assays from mycorrhizal roots were used to characterize the Rir2216-MtWRKY69 interaction. We further analyzed MtWRKY69 expression profile and the contribution of constitutive and conditional MtWRKY69 expression to AMS. We show that Rir2216 is loaded into an AGO1 silencing complex from the host plant M. truncatula, leading to cleavage of a host target transcript encoding for the MtWRKY69 transcription factor. MtWRKY69 is specifically downregulated in arbusculated cells in mycorrhizal roots and increased levels of MtWRKY69 expression led to a reduced AM colonization level. Our results indicate that MtWRKY69 silencing, mediated by a fungal sRNA, is relevant for AMS; we thus present the first experimental evidence of fungus to plant ckRNAi in AMS.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":9.4,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Floral phenotypic divergence and genomic insights in an Ophrys orchid: unraveling early speciation processes. 兰花的花表型差异和基因组见解：揭示早期物种形成过程。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-11-18 DOI: 10.1111/nph.20190

Anaïs Gibert, Bertrand Schatz, Roselyne Buscail, Dominique Nguyen, Michel Baguette, Nicolas Barthes, Joris A M Bertrand

{"title":"Floral phenotypic divergence and genomic insights in an Ophrys orchid: unraveling early speciation processes.","authors":"Anaïs Gibert, Bertrand Schatz, Roselyne Buscail, Dominique Nguyen, Michel Baguette, Nicolas Barthes, Joris A M Bertrand","doi":"10.1111/nph.20190","DOIUrl":"10.1111/nph.20190","url":null,"abstract":"Adaptive radiation in Ophrys orchids leads to complex floral phenotypes that vary in scent, color and shape. Using a novel pipeline to quantify these phenotypes, we investigated trait divergence at early stages of speciation in six populations of Ophrys aveyronensis experiencing recent allopatry. By integrating different genetic/genomic techniques, we investigated: variation and integration of floral components (scent, color and shape); phenotypes and genomic regions under divergent selection; and the genomic bases of trait variation. We identified a large genomic island of divergence, likely associated with phenotypic variation in particular in floral odor. We detected potential divergent selection on macular color, while stabilizing selection was suspected on floral morphology and for several volatile olfactive compounds. We also identified candidate genes involved in anthocyanin and in steroid biosynthesis pathways associated with standing genetic variation in color and odor. This study sheds light on early differentiation in Ophrys, revealing patterns that often become invisible over time, that is the geographic mosaic of traits under selection and the early appearance of strong genomic divergence. It also supports a crucial genomic region for future investigation and highlights the value of a multifaceted approach in unraveling speciation within taxa with large genomes.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":9.4,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Does optimality partitioning theory fail for belowground traits? Insights from geophysical imaging of a drought-release experiment in a Scots Pine forest. 地下性状的最优分区理论是否失效？从苏格兰松树林干旱释放实验的地球物理成像中获得的启示。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-11-18 DOI: 10.1111/nph.20245

Alexis Shakas, Roman Hediger, Arthur Gessler, Kamini Singha, Giulia de Pasquale, Petra D'Odorico, Florian M Wagner, Marcus Schaub, Hansruedi Maurer, Holger Griess, Jonas Gisler, Katrin Meusburger

引用次数: 0

Developmental constraint underlies the replicated evolution of grass awns. 发育约束是禾本科植物芒复制进化的基础。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-11-18 DOI: 10.1111/nph.20268

Erin Patterson, Dana R MacGregor, Michelle M Heeney, Joseph Gallagher, Devin O'Connor, Benedikt Nuesslein, Madelaine Elisabeth Bartlett

{"title":"Developmental constraint underlies the replicated evolution of grass awns.","authors":"Erin Patterson, Dana R MacGregor, Michelle M Heeney, Joseph Gallagher, Devin O'Connor, Benedikt Nuesslein, Madelaine Elisabeth Bartlett","doi":"10.1111/nph.20268","DOIUrl":"https://doi.org/10.1111/nph.20268","url":null,"abstract":"Replicated trait evolution can provide insights into the mechanisms underlying the evolution of biodiversity. One example of replicated evolution is the awn, an organ elaboration in grass inflorescences. Awns are likely homologous to leaf blades. We hypothesized that awns have evolved repeatedly because a conserved leaf blade developmental program is continuously activated and suppressed over the course of evolution, leading to the repeated emergence and loss of awns. To evaluate predictions arising from our hypothesis, we used ancestral state estimations, comparative genetics, anatomy, and morphology to trace awn evolution. We discovered that awned lemmas that evolved independently share similarities in developmental trajectory. In addition, in two species with independently derived awns and differing awn morphologies (Brachypodium distachyon and Alopecurus myosuroides), we found that orthologs of the YABBY transcription factor gene DROOPING LEAF are required for awn initiation. Our analyses of awn development in Brachypodium distachyon, Alopecurus myosuroides, and Holcus lanatus also revealed that differences in the relative expansion of awned lemma compartments can explain diversity in awn morphology at maturity. Our results show that developmental conservation can underlie replicated evolution and can potentiate the evolution of morphological diversity.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":9.4,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Warm temperature perceived at the vegetative stage affects progeny seed germination in natural accessions of Arabidopsis thaliana. 在拟南芥无性繁殖阶段感受到的温暖会影响其后代种子的萌发。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-11-17 DOI: 10.1111/nph.20241

Yu Wang, Tania L Zhang, Emma M Barnett, Sridevi Sureshkumar, Sureshkumar Balasubramanian, Alexandre Fournier-Level

{"title":"Warm temperature perceived at the vegetative stage affects progeny seed germination in natural accessions of Arabidopsis thaliana.","authors":"Yu Wang, Tania L Zhang, Emma M Barnett, Sridevi Sureshkumar, Sureshkumar Balasubramanian, Alexandre Fournier-Level","doi":"10.1111/nph.20241","DOIUrl":"https://doi.org/10.1111/nph.20241","url":null,"abstract":"Temperatures perceived early in the life cycle of mother plants can affect the germination of the offspring seeds. In Arabidopsis thaliana, vernalisation-insensitive mutants showed altered germination response to elevated maternal temperature, hence revealing a strong genetic determinism. However, the genetic control of this maternal effect and its prevalence across natural populations remain unclear. Here, we exposed a collection of European accessions of A. thaliana to increased temperature during the vegetative phase and assessed germination in their progeny to identify the genetic basis of transgenerational germination response. We found that genotypes with rapidly germinating progeny after early maternal exposure to elevated temperature originated from regions with low-light radiation. Combining genome-wide association, expression analysis and functional assays across multiple genetic backgrounds, we show a central role for PHYB in mediating the response to maternally perceived temperature at the vegetative stage. Differential gene expression analysis in leaves identified a similar genetic network as previously found in seed endosperm under elevated temperature, supporting the pleiotropic involvement of PHYB signalling across different tissues and stages. This provides evidence that complex environmental responses modulated by the maternal genotype can rely on a consistent set of genes yet produce different effects at the different stages of exposure.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":9.4,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Origins of xyloglucan-degrading enzymes in fungi. 真菌中木糖降解酶的起源。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-11-17 DOI: 10.1111/nph.20251

Emily D Trudeau, Harry Brumer, Mary L Berbee

{"title":"Origins of xyloglucan-degrading enzymes in fungi.","authors":"Emily D Trudeau, Harry Brumer, Mary L Berbee","doi":"10.1111/nph.20251","DOIUrl":"https://doi.org/10.1111/nph.20251","url":null,"abstract":"The origin story of land plants - the pivotal evolutionary event that paved the way for terrestrial ecosystems of today to flourish - lies within their closest living relatives: the streptophyte algae. Streptophyte cell wall composition has evolved such that profiles of cell wall polysaccharides can be used as taxonomic markers. Since xyloglucan is restricted to the streptophyte lineage, we hypothesized that fungal enzymes evolved in response to xyloglucan availability in streptophyte algal or land plant cell walls. The record of the origins of these enzymes is embedded in fungal genomes, and comparing genomes of fungi that share an ancient common ancestor can provide insights into fungal interactions with early plants. This Viewpoint contributes a review of evidence underlying current assumptions about the distribution of xyloglucan in plant and algal cell walls. We evaluate evolutionary scenarios that may have given rise to the observed distribution of putative xyloglucanases in fungi and discuss possible biological contexts in which these enzymes could have evolved. Our findings suggest that fungal xyloglucanase evolution was more likely driven by land plant diversification and biomass accumulation than by the first origins of xyloglucan in streptophyte algal cell walls.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":9.4,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A defensive pathway from NAC and TCP transcription factors activates a BAHD acyltransferase for (Z)-3-hexenyl acetate biosynthesis to resist herbivore in tea plant (Camellia sinensis). 来自 NAC 和 TCP 转录因子的防御途径可激活 BAHD 乙酰转移酶，促进 (Z)-3- 己烯基乙酸酯的生物合成，从而抵御茶树（Camellia sinensis）中的草食动物。

IF 9.4 1区生物学

New Phytologist Pub Date : 2024-11-17 DOI: 10.1111/nph.20283

Honglian Gu, Jiaxing Li, Dahe Qiao, Mei Li, Yingjie Yao, Hui Xie, Ke-Lin Huang, Shengrui Liu, De-Yu Xie, Chaoling Wei, Junyan Zhu

{"title":"A defensive pathway from NAC and TCP transcription factors activates a BAHD acyltransferase for (Z)-3-hexenyl acetate biosynthesis to resist herbivore in tea plant (Camellia sinensis).","authors":"Honglian Gu, Jiaxing Li, Dahe Qiao, Mei Li, Yingjie Yao, Hui Xie, Ke-Lin Huang, Shengrui Liu, De-Yu Xie, Chaoling Wei, Junyan Zhu","doi":"10.1111/nph.20283","DOIUrl":"https://doi.org/10.1111/nph.20283","url":null,"abstract":"Numerous herbivore-induced plant volatiles (HIPVs) play important roles in plant defense. In tea plants (Camellia sinensis), (Z)-3-hexenyl acetate (3-HAC) has been characterized as associated with resistance to herbivores. To date, how tea plants biosynthesize and regulate 3-HAC to resist herbivores remain unclear. Based on transcriptomes assembled from Ectropis obliqua-fed leaves, a cDNA encoding BAHD acyltransferase, namely CsCHAT1, was highly induced in leaves fed with E. obliqua. Enzymatic assays showed that CsCHAT1 converted (Z)-3-hexenol into 3-HAC. Further suppression of CsCHAT1 expression reduced the accumulation of 3-HAC and lowered the resistance of tea plants to E. obliqua, while 3-HAC replenishment rescued the reduced resistance of CsCHAT1-silenced tea plants against E. obliqua. Two transcription factors (TFs), CsNAC30 and CsTCP11, were co-expressed with CsCHAT1. An integrative approach of biochemistry, DNA-protein interaction, gene silencing, and metabolic profiling revealed that the two TFs positively regulated the expression of CsCHAT1. The suppression of either one decreased the production of 3-HAC and eliminated the resistance of tea plants to E. obliqua. Notably, the suppression of either one considerably impaired JA-induced 3-HAC biosynthesis in tea plant. The proposed pathway can be targeted for innovative agro-biotechnologies protecting tea plants from damage by E. obliqua.","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":9.4,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0