OsZNHIT3 functions as a transcriptional activator to regulate tapetal programmed cell death and exine formation in rice.

IF 9.4 1区 生物学 Q1 Agricultural and Biological Sciences
New Phytologist Pub Date : 2025-07-04 DOI:10.1111/nph.70366
Wang Wang, Feng Liu, Haodong Shen, Tiancheng Hao, Xiaoyun Tan, Kun Chen, Tianqi Duan, Jingyi Li, Liyan Bu, Shiqi Song, Nan Wang, Huiting Yang, Yifeng Xu, Huadong Zhan, Yiqun Bao
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Abstract

Proper timing of programmed cell death (PCD) in the tapetum and the deposition of pollen wall are crucial for gametophyte development. Although several genes involved in these processes have been identified, the regulatory mechanisms remain poorly understood. We isolated and characterized a rice male-sterile mutant, exine abnormal 1 (exa1), which exhibits pale anthers and aborted pollen. The exa1 mutant displays delayed tapetal PCD and defective pollen wall patterning, including thinner sexine and thicker nexine. EXA1 (Exine Abnormal 1), preferentially expressed in the tapetum and microspores, encodes a novel Zf-HIT (Zinc finger-High Temperature) family protein, OsZNHIT3. Nucleus and cytoplasm dual-localized EXA1 functions as a transcriptional activator via its previously undefined C-terminal domain. Phenotypic similarities in rice expressing EXA1-SRDX (SUPERMAN Repressive Domain X) fusion proteins further support its role as a transcriptional activator. PTC1 (Persistent Tapetal Cell 1), EAT1 (Eternal Tapetum 1), and their downstream genes were significantly downregulated in exa1 mutant anthers. Introducing pEXA1:PTC1 or pEXA1:EAT1 into the exa1 mutant partially rescued anther appearance, pollen morphology, and sexine-nexine ratio. This suggests that EXA1 acts genetically upstream of PTC1 and EAT1 in the regulatory pathway. Our study reveals a novel and essential role for EXA1 as a transcriptional activator in rice anther development.

OsZNHIT3作为转录激活因子调控水稻绒毡层程序性细胞死亡和外叶形成。
绒毡层细胞程序性死亡(PCD)和花粉壁沉积的时机对配子体发育至关重要。虽然已经确定了参与这些过程的几个基因,但对其调控机制仍知之甚少。我们分离并鉴定了一个水稻雄性不育突变体exine abnormal 1 (exa1),其花药苍白,花粉败育。exa1突变体表现出绒毡层PCD延迟和花粉壁图案缺陷,包括较薄的性壁和较厚的内壁。EXA1 (Exine Abnormal 1)优先表达于绒毡层和小孢子中,编码一种新的Zf-HIT(锌指-高温)家族蛋白OsZNHIT3。细胞核和细胞质双定位的EXA1通过其先前未定义的c端结构域作为转录激活因子发挥作用。在表达EXA1-SRDX (SUPERMAN suppression Domain X)融合蛋白的水稻中,表型相似性进一步支持了其作为转录激活因子的作用。PTC1 (Persistent Tapetal Cell 1)、EAT1 (Eternal Tapetum 1)及其下游基因在exa1突变花药中显著下调。将pEXA1:PTC1或pEXA1:EAT1导入到exa1突变体中,部分恢复了花药外观、花粉形态和两性间质比例。这表明EXA1在调控途径中作用于PTC1和EAT1的遗传上游。我们的研究揭示了EXA1作为转录激活因子在水稻花药发育中的重要作用。
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来源期刊
New Phytologist
New Phytologist PLANT SCIENCES-
CiteScore
17.60
自引率
5.30%
发文量
728
审稿时长
1 months
期刊介绍: New Phytologist is a leading publication that showcases exceptional and groundbreaking research in plant science and its practical applications. With a focus on five distinct sections - Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology - the journal covers a wide array of topics ranging from cellular processes to the impact of global environmental changes. We encourage the use of interdisciplinary approaches, and our content is structured to reflect this. Our journal acknowledges the diverse techniques employed in plant science, including molecular and cell biology, functional genomics, modeling, and system-based approaches, across various subfields.
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