Semiautomatic quantification of 3D Histone H3 phosphorylation signals during cell division in Arabidopsis root meristems.

IF 8.1 1区 生物学 Q1 Agricultural and Biological Sciences
New Phytologist Pub Date : 2025-07-07 DOI:10.1111/nph.70365
Adrienn Kelemen, Magalie Uyttewaal, Csaba Máthé, Philippe Andrey, David Bouchez, Martine Pastuglia
{"title":"Semiautomatic quantification of 3D Histone H3 phosphorylation signals during cell division in Arabidopsis root meristems.","authors":"Adrienn Kelemen, Magalie Uyttewaal, Csaba Máthé, Philippe Andrey, David Bouchez, Martine Pastuglia","doi":"10.1111/nph.70365","DOIUrl":null,"url":null,"abstract":"<p><p>Posttranslational modification of histones during the cell cycle is a major process controlling many aspects of cell division. Among the variety of histone modifications, mitotic phosphorylation of histone H3 at serine 10 (H3S10ph) plays a crucial role, particularly in proper chromosome segregation. Here we aimed at precisely quantifying this phosphorylation dynamics during mitosis in plant cells in order to reveal molecular pathways involved in this process. We describe an analysis pipeline based on 3D image analysis that allows to semiautomatically quantify H3S10ph in mitotic Arabidopsis root cells. We also developed a new method for the compensation of signal attenuation in Z, based on measurement of objects of interest themselves. We show that this new attenuation correction method allows significant gains in accuracy and statistical power. Using this pipeline, we were able to reveal small H3S10ph differences between cells treated with hesperadin, an inhibitor of an H3S10ph kinase, or between Arabidopsis mutants affected in PP2A phosphatase activity. This tool opens new avenues to explore such regulatory pathways in plants, using the wealth of genetic materials available in Arabidopsis. It can also be applied to study other histone posttranslational modifications and more generally to any discrete 3D signals.</p>","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":" ","pages":""},"PeriodicalIF":8.1000,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"New Phytologist","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/nph.70365","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0

Abstract

Posttranslational modification of histones during the cell cycle is a major process controlling many aspects of cell division. Among the variety of histone modifications, mitotic phosphorylation of histone H3 at serine 10 (H3S10ph) plays a crucial role, particularly in proper chromosome segregation. Here we aimed at precisely quantifying this phosphorylation dynamics during mitosis in plant cells in order to reveal molecular pathways involved in this process. We describe an analysis pipeline based on 3D image analysis that allows to semiautomatically quantify H3S10ph in mitotic Arabidopsis root cells. We also developed a new method for the compensation of signal attenuation in Z, based on measurement of objects of interest themselves. We show that this new attenuation correction method allows significant gains in accuracy and statistical power. Using this pipeline, we were able to reveal small H3S10ph differences between cells treated with hesperadin, an inhibitor of an H3S10ph kinase, or between Arabidopsis mutants affected in PP2A phosphatase activity. This tool opens new avenues to explore such regulatory pathways in plants, using the wealth of genetic materials available in Arabidopsis. It can also be applied to study other histone posttranslational modifications and more generally to any discrete 3D signals.

拟南芥根分生组织细胞分裂过程中组蛋白H3磷酸化信号的半自动定量分析。
在细胞周期中,组蛋白的翻译后修饰是控制细胞分裂许多方面的主要过程。在各种组蛋白修饰中,组蛋白H3丝氨酸10位点的有丝分裂磷酸化(H3S10ph)起着至关重要的作用,特别是在正确的染色体分离中。在这里,我们旨在精确量化植物细胞有丝分裂过程中的磷酸化动力学,以揭示参与这一过程的分子途径。我们描述了一个基于3D图像分析的分析管道,允许半自动量化有丝分裂拟南芥根细胞中的H3S10ph。我们还开发了一种新的方法来补偿信号衰减在Z,基于测量感兴趣的对象本身。我们表明,这种新的衰减校正方法可以显著提高精度和统计功率。使用这个管道,我们能够揭示用橙皮苷(H3S10ph激酶抑制剂)处理的细胞之间或在PP2A磷酸酶活性受影响的拟南芥突变体之间H3S10ph的微小差异。该工具利用拟南芥中丰富的遗传物质,为探索植物中的这种调控途径开辟了新的途径。它也可以应用于研究其他组蛋白翻译后修饰,更广泛地应用于任何离散的3D信号。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
New Phytologist
New Phytologist PLANT SCIENCES-
CiteScore
17.60
自引率
5.30%
发文量
728
审稿时长
1 months
期刊介绍: New Phytologist is a leading publication that showcases exceptional and groundbreaking research in plant science and its practical applications. With a focus on five distinct sections - Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology - the journal covers a wide array of topics ranging from cellular processes to the impact of global environmental changes. We encourage the use of interdisciplinary approaches, and our content is structured to reflect this. Our journal acknowledges the diverse techniques employed in plant science, including molecular and cell biology, functional genomics, modeling, and system-based approaches, across various subfields.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信