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Eucalyptus grandis Forestry Residue Valorization: Distinct and Integrated Pretreatment Methods for Enhanced Xylooligosaccharide Production 桉树林业残留物的价值评估:提高低聚木糖生产的独特综合预处理方法
IF 3.1 3区 工程技术
BioEnergy Research Pub Date : 2024-04-13 DOI: 10.1007/s12155-024-10748-1
Jenniffer Andrea Tamayo-Peña, Laura Plazas Tovar, Lívia Caldas Alencar Pacheco, Adilson Roberto Gonçalves, Telma Texeira Franco
{"title":"Eucalyptus grandis Forestry Residue Valorization: Distinct and Integrated Pretreatment Methods for Enhanced Xylooligosaccharide Production","authors":"Jenniffer Andrea Tamayo-Peña,&nbsp;Laura Plazas Tovar,&nbsp;Lívia Caldas Alencar Pacheco,&nbsp;Adilson Roberto Gonçalves,&nbsp;Telma Texeira Franco","doi":"10.1007/s12155-024-10748-1","DOIUrl":"10.1007/s12155-024-10748-1","url":null,"abstract":"<div><p>Eucalyptus branches and bark represent highly abundant and available feedstocks with great potential for obtaining bio-based products. Distinct and integrated pretreatment fractionation strategies for eucalyptus branches and bark were performed for the efficient production of xylooligosaccharides (XOS). By combining pretreatments, a high yield of XOS was obtained from eucalyptus branches and bark. The branches and bark were presoaked in 8% (w/w) sodium hydroxide at 60 °C for 30 min to provide a deacetylation effect. The residues were then hydrothermally treated. The findings revealed that 4.64% of XOS originated from the bark and 6.19% from eucalyptus branches. It has been demonstrated that xylan may be selectively depolymerized during pretreatment by preventing excessive hydrolysis through the use of deacetylation in the first phase of the process. More XOS was produced using hydrothermal treatment, yielding 8.00% (w/w) in the branches and 5.12% in the bark. A significant amount of XOS with DP 2–5 might be obtained in certain experiments, up to 60%, but the most abundant XOS are usually those with DP &gt; 5 (approximately 80% of all XOS). This work provides new insights into the effective generation of XOS under relatively mild conditions by overcoming the recalcitrant structure of eucalyptus branches and bark, representing a noteworthy advancement towards forestry leftover valorization.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"17 3","pages":"1503 - 1521"},"PeriodicalIF":3.1,"publicationDate":"2024-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12155-024-10748-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140590693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cultivation of Microalgae in Liquid Digestate to Remove Nutrients and Organic Contaminants 在液态消化液中培养微藻以去除营养物质和有机污染物
IF 3.1 3区 工程技术
BioEnergy Research Pub Date : 2024-04-13 DOI: 10.1007/s12155-024-10753-4
Ewelina Sobolewska, Sebastian Borowski, Paulina Nowicka-Krawczyk
{"title":"Cultivation of Microalgae in Liquid Digestate to Remove Nutrients and Organic Contaminants","authors":"Ewelina Sobolewska,&nbsp;Sebastian Borowski,&nbsp;Paulina Nowicka-Krawczyk","doi":"10.1007/s12155-024-10753-4","DOIUrl":"10.1007/s12155-024-10753-4","url":null,"abstract":"<div><p>The goal of this research was to assess the efficiency of the liquid digestate treatment conducted with algal, environmental isolates illuminated entirely with sunlight. The photobioreactor was exposed to natural conditions and evaluated based on the reduction of chemical oxygen demand (COD), nitrogen compounds, and soluble phosphates. Microalgal and bacterial communities growing during the treatment process were studied. A high removal rate of soluble COD (= 91%) and nutrients (= 86%) was achieved. The average concentrations of nitrogen, phosphates, and COD in the reactor effluent were 95 mgN/L, 49 mg/L, and 735 mg O<sub>2</sub>/L, respectively. The overall algae-bacteria biomass productivity of 22 mg/L/d, calculated on the total suspended solids (TSS) basis, was recorded. The microbial analysis revealed the dominance of <i>Tetradesmus obliquus</i> followed by <i>Microglena</i> sp. in the first 14 weeks of the experiment. At the end of the experimental run, <i>Chlorella sorokiniana</i> cells appeared as a result of illumination intensity changes. The dominating bacteria belonged to Firmicutes (26.31%), Patescibacteria (17.38%), and Actinobacteriota (14.86%) and could have been responsible for the transformation of nitrogen and oxidation of organic contaminants. The research demonstrated that natural sunlight can successfully be used for efficient liquid digestate treatment with the algae-bacterial community.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"17 3","pages":"1843 - 1855"},"PeriodicalIF":3.1,"publicationDate":"2024-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12155-024-10753-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140590412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differentiating Bagasse and Straw as Feedstocks for Sugarcane Cellulosic Ethanol: Insights from Pilot-Scale Pretreatments 甘蔗渣和秸秆作为甘蔗纤维素乙醇原料的区别:中试预处理的启示
IF 3.1 3区 工程技术
BioEnergy Research Pub Date : 2024-04-13 DOI: 10.1007/s12155-024-10751-6
Viviane M. Nascimento, Simone C. Nakanishi, Carlos Alberto de Oliveira Filho, Absai da Conceição Gomes, Aline Machado de Castro, Ana Paula Rodrigues Torres, Christian Alejandro Queipo, Danuza Nogueira Moyses, Felipe de Oliveira Brito, Luiz Fernando Martins Bandeira, Carlos Driemeier
{"title":"Differentiating Bagasse and Straw as Feedstocks for Sugarcane Cellulosic Ethanol: Insights from Pilot-Scale Pretreatments","authors":"Viviane M. Nascimento,&nbsp;Simone C. Nakanishi,&nbsp;Carlos Alberto de Oliveira Filho,&nbsp;Absai da Conceição Gomes,&nbsp;Aline Machado de Castro,&nbsp;Ana Paula Rodrigues Torres,&nbsp;Christian Alejandro Queipo,&nbsp;Danuza Nogueira Moyses,&nbsp;Felipe de Oliveira Brito,&nbsp;Luiz Fernando Martins Bandeira,&nbsp;Carlos Driemeier","doi":"10.1007/s12155-024-10751-6","DOIUrl":"10.1007/s12155-024-10751-6","url":null,"abstract":"<div><p>Bagasse and straw are the two feedstocks used to produce cellulosic ethanol from sugarcane. Although this technology is advancing commercially, the differentiation between bagasse and straw remains elusive. This work investigates bagasse and straw supply, conditioning, and pretreatment in a pilot-scale steam-explosion continuous reactor (~ 10 kg/h feed) to illuminate the critical feedstock differences for process scale-up. Evaluating biomass across four sequential harvest seasons (2018–2021) shows that straw supplied in bales requires extra conditioning to reduce mineral matter and particle size before feeding into the reactor. Moreover, straw composition is more variable and consistently has lower contents of glucan (35.6–38.8%) and carbohydrate potential (glucose + xylose, 649–704 kg/dry tonne) compared to bagasse (40.8–42.9%; 735–760 kg/dry tonne). Biomass pretreatments without (190 °C, 5–15 min) and with sulfuric acid catalyst (149–170 °C; 5–15 min; 0.5–5.12%, m/m) show that straw differs from bagasse by presenting a higher acid neutralization capacity and about 5% of labile glucans. These results suggest that straw crushing and aqueous pre-extraction are promising strategies to reduce the dissimilarities with bagasse, thus facilitating the development of feedstock-agnostic biorefining.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"17 3","pages":"1533 - 1542"},"PeriodicalIF":3.1,"publicationDate":"2024-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140590420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Synergistic of Magnetite Nanoparticles and Alkaline Pretreatment on Biogas Yield from Anaerobic Codigestion of Municipal Waste and Sewage Sludge 磁铁矿纳米颗粒和碱性预处理对城市垃圾和污水污泥厌氧消化产生的沼气产量的协同作用
IF 3.1 3区 工程技术
BioEnergy Research Pub Date : 2024-04-02 DOI: 10.1007/s12155-024-10742-7
Mansour Ahmadi-Pirlou, Amirhamzeh Farajollahi, AhmadReza Rahmati
{"title":"Synergistic of Magnetite Nanoparticles and Alkaline Pretreatment on Biogas Yield from Anaerobic Codigestion of Municipal Waste and Sewage Sludge","authors":"Mansour Ahmadi-Pirlou,&nbsp;Amirhamzeh Farajollahi,&nbsp;AhmadReza Rahmati","doi":"10.1007/s12155-024-10742-7","DOIUrl":"10.1007/s12155-024-10742-7","url":null,"abstract":"<div><p>One of the most important problems and the main obstacle of this process is the transformation of the complex structure of lignocellulose. One of the ways to break the lignocellulosic structure is to use suitable pretreatment compounds and nanoparticles with appropriate concentration. For this purpose, the synergistic effect of 6%NaOH pretreatment with different magnetite nanoparticle (MNP) concentrations was assessed for maximizing the biogas production. In this study, the optimal mixing ratio of MSW and SS codigestion was determined from our previous studies (MSW:SS 60:40). Application of the 6%NaOH pretreatment alone increased biogas and methane production by 24.6 and 35.4%, respectively, compared to the control. Also, the 6%NaOH pretreatment alone elevated cellulose by 85% while reducing lignin and hemicellulose by 64 and 33%, respectively. The greatest biogas and methane production was obtained in the reactor of 6%NaOH with an MNP concentration of 110 ppm, experiencing an 86 and 162% increase compared to the control. The greatest reduction in total solids and volatile solids was also obtained in this digestor by 76.6 and 79%, respectively, compared to the control reactor.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"17 3","pages":"1908 - 1917"},"PeriodicalIF":3.1,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140590411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influence of Ensiling Time and Elephant Grass Silage Alkaline Pretreatment in Anaerobic Co-digestion with Vinasse for Methane Production 饲养时间和象草青贮碱性预处理对厌氧协同消化沼气生产的影响
IF 3.1 3区 工程技术
BioEnergy Research Pub Date : 2024-03-26 DOI: 10.1007/s12155-024-10746-3
Heloisa Vital Domingos, Thayse Farias de Barros, Taciana Carneiro Chaves, Fernanda Santana Peiter, Dayana de Gusmão Coêlho, Anderson Carlos Marafon, Eduardo Lucena Cavalcante de Amorim
{"title":"Influence of Ensiling Time and Elephant Grass Silage Alkaline Pretreatment in Anaerobic Co-digestion with Vinasse for Methane Production","authors":"Heloisa Vital Domingos,&nbsp;Thayse Farias de Barros,&nbsp;Taciana Carneiro Chaves,&nbsp;Fernanda Santana Peiter,&nbsp;Dayana de Gusmão Coêlho,&nbsp;Anderson Carlos Marafon,&nbsp;Eduardo Lucena Cavalcante de Amorim","doi":"10.1007/s12155-024-10746-3","DOIUrl":"10.1007/s12155-024-10746-3","url":null,"abstract":"<div><p>This study investigated the anaerobic co-digestion of sugarcane vinasse (V) and elephant grass silage (S) to produce methane. Box-Behnken experimental design was applied to verify the statistical effects of the elephant grass ensiling time (40, 80 and 120 days), alkaline pretreatment of elephant grass silage (0.5, 2.25 and 4.00% w/v NaOH) and S:V mixture ratio (25:75, 50:50 and 75:25) on the methane yield. The results showed that the ensiling process resulted in the low degradation of lignocellulosic substances, emphasizing the need for pretreatment using more efficient techniques, such as thermo-alkaline, to improve the breakdown of elephant grass fibres. COD removals varied between 35 and 85%, and carbohydrate consumptions ranged from 63 to 72%, with the higher efficiencies for both parameters occurring in the reactors with lower percentages of silage. Cumulative methane yield ranged from 190.77 mLCH<sub>4</sub>/gVS (in the reactor with S:V of 75:25, 0.50% w/v NaOH and 80 ensiling days) to 1729.80 mLCH<sub>4</sub>/gVS (in the reactor with S:V of 25:75, 2.25% w/v NaOH and 120 ensiling days). According to ANOVA, S:V ratio was the only variable with a significant effect (<i>p</i> &lt; 0.05) on cumulative methane yield. Therefore, the findings indicate that the relative composition of substrates within the mixture exerted the most significant influence on the process, underscoring the critical role of vinasse as a co-substrate in enhancing methane production despite silage pretreatments.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"17 3","pages":"1895 - 1907"},"PeriodicalIF":3.1,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140301725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Machine Learning Modelling for Predicting the Efficacy of Ionic Liquid-Aided Biomass Pretreatment 预测离子液体辅助生物质预处理功效的机器学习模型
IF 3.1 3区 工程技术
BioEnergy Research Pub Date : 2024-03-26 DOI: 10.1007/s12155-024-10747-2
Biswanath Mahanty, Munmun Gharami, Dibyajyoti Haldar
{"title":"Machine Learning Modelling for Predicting the Efficacy of Ionic Liquid-Aided Biomass Pretreatment","authors":"Biswanath Mahanty,&nbsp;Munmun Gharami,&nbsp;Dibyajyoti Haldar","doi":"10.1007/s12155-024-10747-2","DOIUrl":"10.1007/s12155-024-10747-2","url":null,"abstract":"<div><p>The influence of ionic liquid (IL) characteristics, lignocellulosic biomass (LCB) properties, and process conditions on LCB pretreatment is not well understood. In this study, a total of 129 experimental data on LCB (grass, agricultural, and forest residues) pretreatment using imidazolium, triethylamine, and choline-amino acid ILs were compiled to develop machine learning (ML) models for cellulose, hemicellulose, lignin, and solid recovery. Following data imputation, a bilayer artificial neural network (ANN) and random forest (RF) regression, the two most widely adopted ML models, were developed. The full-featured ANN following Bayesian hyperparameter (HP) optimisation offered excellent fit on training (<i>R</i><sup>2</sup>: 0.936–0.994), though cross-validation (<i>R</i><sub>2</sub>CV) performance remained marginally poor, i.e. between 0.547 and 0.761. The fitness of HP-optimised RF models varied between 0.824 and 0.939 for regression, and between 0.383 and 0.831 in cross-validation. Temperature and pretreatment time had been the most important predictors, except for hemicellulose recovery. Bayesian predictor selection combined with HP optimisation improved the <i>R</i><sup>2</sup>CV boundary for ANN (0.555–0.825), as well as for RF models (0.474–0.824). As predictive performance of the models varied depending on target response, use of a larger homogeneous dataset may be warranted. The predictive modelling framework for LCB pretreatment, developed in this study, can be extended to similar biochemical process systems.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"17 3","pages":"1569 - 1583"},"PeriodicalIF":3.1,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140303207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Hemicelluloses Fractionated by Graded Ethanol Precipitation from Corn Stover on the Enzymatic Hydrolysis of Lignocellulosic Biomass 玉米秸秆乙醇分级沉淀法提取的半纤维素对木质纤维素生物质酶水解的影响
IF 3.1 3区 工程技术
BioEnergy Research Pub Date : 2024-03-25 DOI: 10.1007/s12155-024-10745-4
Zhanqiang Yan, Md Asraful Alam, Jinfeng Li, Wenlong Xiong, Shen Zhang, Zili Zhan, Jingliang Xu
{"title":"Effect of Hemicelluloses Fractionated by Graded Ethanol Precipitation from Corn Stover on the Enzymatic Hydrolysis of Lignocellulosic Biomass","authors":"Zhanqiang Yan,&nbsp;Md Asraful Alam,&nbsp;Jinfeng Li,&nbsp;Wenlong Xiong,&nbsp;Shen Zhang,&nbsp;Zili Zhan,&nbsp;Jingliang Xu","doi":"10.1007/s12155-024-10745-4","DOIUrl":"10.1007/s12155-024-10745-4","url":null,"abstract":"<div><p>The presence of hemicellulose inhibits the enzymatic hydrolysis of lignocellulosic biomass. The purpose of this study is to investigate the effect of different hemicellulose fractions on the enzymatic hydrolysis and the way to eliminate the inhibiting effect caused by hemicellulose. Four kinds of hemicelluloses, namely, H<sub>XF</sub>, H<sub>15</sub>, H<sub>30</sub>, and H<sub>60</sub>, were first extracted from corn stover by ethanol fractional precipitation. The structures of hemicellulose samples were analyzed using Fourier transform infrared spectroscopy, <sup>1</sup>H and <sup>13</sup>C nuclear magnetic resonance, and high-performance ion chromatography. The results show that H<sub>30</sub> has the strongest inhibition on the enzymatic hydrolysis of Avicel and corn stover, presenting inhibition ratio of 13.35% and 9.98%, respectively. The inhibition ratios of other hemicelluloses in Avicel and corn stover are 8–12% and 5–9%, respectively. However, the inhibiting effect caused by H<sub>30</sub> is removed by adding hemicellulase, which even presents a 4.99% increase in the efficiency of enzymatic hydrolysis of corn stover. The corresponding glucose concentration reached 68.11 g/L. This research could help design effective processes to promote the enzymatic hydrolysis of lignocellulosic biomass.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"17 3","pages":"1469 - 1480"},"PeriodicalIF":3.1,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140301858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Use of Cranberry Bush (Viburnum opulus L.) Fruit Pomace as a Renewable Substrate for Biobutanol Production by Clostridium beijerinckii in the Presence of Sodium Dithionite 利用蔓越橘果渣作为可再生底物,在亚硫酸钠存在下通过贝氏梭菌生产生物丁醇
IF 3.1 3区 工程技术
BioEnergy Research Pub Date : 2024-03-22 DOI: 10.1007/s12155-024-10739-2
Mariye Nur Çelik, Nazlıhan Tekin, Sevgi Ertuğrul Karatay, Gönül Dönmez
{"title":"Use of Cranberry Bush (Viburnum opulus L.) Fruit Pomace as a Renewable Substrate for Biobutanol Production by Clostridium beijerinckii in the Presence of Sodium Dithionite","authors":"Mariye Nur Çelik,&nbsp;Nazlıhan Tekin,&nbsp;Sevgi Ertuğrul Karatay,&nbsp;Gönül Dönmez","doi":"10.1007/s12155-024-10739-2","DOIUrl":"10.1007/s12155-024-10739-2","url":null,"abstract":"<div><p>The present study aims to evaluate the use of cranberry bush fruit pomace (CBFP) (<i>Viburnum opulus</i> L.), which has recently become popular raw material, as a substrate in the presence of a reducing agent to increase biobutanol production by <i>Clostridium beijerinckii</i> DSMZ 6422. For this purpose, some factors were optimized, including the pretreatment, initial concentration of CBFP (5–20%), different types of reducing agents (ascorbic acid, L-cysteine, sodium dithionite and sodium sulfite), different concentrations of sodium dithionite (2.5–15 mM), inoculum concentration (5%, 10%, and 20%), and fermentation time (24–96 h). The maximum biobutanol, total ABE, biobutanol yield, and biobutanol productivity were 9.45 g/L, 12.08 g/L, 0.21 g/g, and 0.13 g/L/h in the medium containing enzymatically hydrolyzed 10% CBFP, 10 mM sodium dithionite, and 20% inoculum at the end of 72 h, respectively. These findings demonstrate that CBFP can be considered as a sustainable, economical, and viable substrate on biobutanol production for the first time in the literature.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"17 3","pages":"1689 - 1700"},"PeriodicalIF":3.1,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12155-024-10739-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140197455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Self-Buffering Effect of Solids During High-Solid Enzymatic Hydrolysis of Lignocellulose 木质纤维素高固酶水解过程中固体的自我缓冲作用
IF 3.1 3区 工程技术
BioEnergy Research Pub Date : 2024-03-19 DOI: 10.1007/s12155-024-10744-5
Xin Shi, Lan Wang, Hongzhang Chen
{"title":"Self-Buffering Effect of Solids During High-Solid Enzymatic Hydrolysis of Lignocellulose","authors":"Xin Shi,&nbsp;Lan Wang,&nbsp;Hongzhang Chen","doi":"10.1007/s12155-024-10744-5","DOIUrl":"10.1007/s12155-024-10744-5","url":null,"abstract":"<div><p>The role of buffer in modulating the enzymatic hydrolysis environment of lignocellulose is crucial. However, studies on the impact of buffer on high-solid enzymatic hydrolysis remain limited. This study discovered that utilizing deionized water as a reaction medium, rather than the conventional buffer, did not influence the enzymatic hydrolysis of steam-exploded corn stover when the solid loading ranged between 15 and 25%. At 15% solid loading, the glucan conversion in the group treated with buffer was recorded at 89.8%, with a corresponding glucose concentration of 51.1 g/L. In contrast, the group without buffer exhibited a conversion of 88.9% and a glucose concentration of 50.5 g/L. The increase of acid groups in lignin was attributed to the formation of phenolic hydroxyls during steam explosion, which provided the substrate with the necessary conditions for buffering effects. Sequentially, during the high-solid enzymatic hydrolysis process, the substrate’s increased pore volume and specific surface area could potentially offset the buffering capacity, which led to the buffering effect becoming ineffective. Leveraging the self-buffering effect of the substrate, a fed-batch strategy was developed. This strategy replaced the water supplementation with buffers, augmenting the solid loading from 20 to 33% across six distinct feeding sessions over a span of 72 h. This not only reduced costs but also laid the foundation for the industrial viability of lignocellulosic high-concentration sugar production, thereby advancing the biofuels and bioproducts sector. These findings provide valuable insights for the exploration of solid reaction processes.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"17 3","pages":"1701 - 1711"},"PeriodicalIF":3.1,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140171141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Unveiling the Biomass Valorization: The Microbial Diversity in Promoting a Sustainable Socio-economy 揭示生物质的价值:促进可持续社会经济的微生物多样性
IF 3.1 3区 工程技术
BioEnergy Research Pub Date : 2024-03-15 DOI: 10.1007/s12155-024-10743-6
Antonielle Vieira Monclaro, Helder Andrey Rocha Gomes, Gilvan Caetano Duarte, Leonora Rios de Souza Moreira, Edivaldo Ximenes Ferreira Filho
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