BioEnergy Research最新文献

{"title":"Dual-Acidity Catalysts for Alkyl Levulinate Synthesis from Biomass Carbohydrates: A Review","authors":"Kirrthana Krishnasamy,&nbsp;Mohd Asmadi,&nbsp;Muzakkir Mohammad Zainol,&nbsp;Nor Aishah Saidina Amin,&nbsp;Zaki Yamani Zakaria,&nbsp;Sureena Binti Abdullah","doi":"10.1007/s12155-024-10726-7","DOIUrl":"10.1007/s12155-024-10726-7","url":null,"abstract":"<div><p>To combat the fast-degrading environment in this era, efforts are being made to consider biomass as feedstock for the production of biofuels and biofuel additives. Alkyl levulinates (AL), a good biofuel additive, exhibit excellent performance in improving the flow properties of biofuels. The growing market value of AL makes it highly desirable for large-scale productions. Unfortunately, the highly complex structure of biomass often hinders its conversion to AL, limiting its production. Thus, finding solutions to achieve highly efficient biomass conversion to AL is crucial. Catalyst dual-acidity is important in determining conversion effectiveness. The applications of AL, the roles of the catalyst acidic type in facilitating biomass carbohydrates conversions to AL, the types of catalysts employed and their impact, the prominence of the catalyst dual acidity, and the effect of reaction conditions on the conversion process are discussed. Dual-acidity Brønsted-Lewis catalyst enhances the AL yields and selectivity from carbohydrate conversion. Dual-acidity catalysts in the form of homogenous and heterogenous exhibited several advantages and disadvantages, which are compared in terms of catalytic performance and cost-effectiveness. Carbon-based catalysts are suggested as the prominent choice for large-scale AL production from carbohydrates. The outcome of this review will provide insights into the advancements in AL production from biomass carbohydrates.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"17 2","pages":"790 - 815"},"PeriodicalIF":3.1,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140003022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pulsed drip irrigation reduces sugarcane water consumption and improves growth, productivity, sugar and ethanol yields","authors":"Sirleide Maria de Menezes,&nbsp;Gerônimo Ferreira da Silva,&nbsp;Manassés Mesquita da Silva,&nbsp;José Edson Florentino de Morais,&nbsp;Maria Catiana de Vasconcelos,&nbsp;Carolayne Silva de Souza,&nbsp;Djalma Euzébio Simões Neto,&nbsp;Mário Monteiro Rolim","doi":"10.1007/s12155-024-10729-4","DOIUrl":"10.1007/s12155-024-10729-4","url":null,"abstract":"<div><p>The water deficit resulting from climate variations limits the profitability and sustainability of sugarcane fields, making water supply through irrigation necessary to sustain the potential production of sugarcane. However, the water used for irrigation purposes must be properly managed, ensuring the conservation of water resources and the reduction of costs with the use of inputs and energy. Pulsed drip irrigation aims to support irrigation management, improving the efficient use of water and mitigating the deleterious effects of water deficit. This study aims to evaluate the growth, productivity, and industrial yield of sugarcane cultivated under continuous and pulsed drip irrigation. A field experiment was conducted at the Experimental Sugarcane Station of Carpina, in Carpina in the State of Pernambuco, Northeast Brazil, from December 2020 to December 2021. The experimental arrangement was randomized blocks in a 2 x 5 factorial design, with two types of irrigation application (pulsed and continuous) and five irrigation levels (40, 60, 80, 100, and 120% of crop evapotranspiration – ETc), with four replications. Pulsed drip irrigation increased the yield of stalks (9%) and sugar (21%) in the sugarcane crop and ethanol (17%) derived from sugar in the juice. Pulsed drip irrigation, when compared to continuous irrigation, improved the performance of sugarcane, providing a reduction in water consumption and increasing growth, stalk yield, sugar and predicted ethanol yield. Thus, based on this study, pulse irrigation is an efficient approach to irrigation management, contributing to the stability of sugarcane production while conserving water relative to continuous irrigation.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"17 3","pages":"1413 - 1424"},"PeriodicalIF":3.1,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139981182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hemicellulose Biomass Degree of Acetylation (Natural Versus Chemical Acetylation) as a Strategy for Based Packaging Materials","authors":"Júlia Ribeiro Martins,&nbsp;Jaiber Humberto Rodriguez Llanos,&nbsp;Vagner Botaro,&nbsp;Adilson Roberto Gonçalves,&nbsp;Michel Brienzo","doi":"10.1007/s12155-024-10734-7","DOIUrl":"10.1007/s12155-024-10734-7","url":null,"abstract":"<div><p>Facing increasing social, environmental, and economic pressure to substitute non-renewable fossil resources with renewable ones, hemicellulose has received attention as a substrate for the production of high-value products such as packaging materials because of its non-toxicity, abundance, and biodegradability. Hemicelluloses in the cell wall are naturally substituted with acetyl groups, and the degree and pattern of acetylation vary among plant species, tissue and cell types, and plant maturity. Hemicellulose acetylation influences features such as the flexural properties of wood, polysaccharide interactions, plant growth, and stress resistance. However, hemicellulose is deacetylated during its separation from other biomass polymers, mainly via alkaline solubilization. Therefore, when industrial applications require a certain degree of acetylation, chemical acetylation is necessary, which occurs through an esterification reaction that links acetyl groups to hemicellulose, catalyzed or not. Acetylation may enhance some features of hemicellulose-based packaging materials, such as mechanical strength, processability, thermal stability, hydrophobicity, and oxygen and water vapor permeability. This review provides an update on the latest advances in plant polysaccharide acetylation, including the acetylation mechanism in the plant cell wall as well as the influence of such esterification on plant properties and wood industrial application. Recent developments and progress in hemicellulose chemical acetylation strategies have been summarized, disclosing the advantages and disadvantages of different solvents and catalysts applied and acetylation evaluation methods.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"17 2","pages":"877 - 896"},"PeriodicalIF":3.1,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140007650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-Pyrolysis of Peanut Shells and Tea Plant Branches: Physicochemical Properties, Synergistic Effect and Thermo-Kinetic Analyses","authors":"Tarique Ahmed Memon,&nbsp;Xiaoke Ku,&nbsp;Vikul Vasudev","doi":"10.1007/s12155-024-10728-5","DOIUrl":"10.1007/s12155-024-10728-5","url":null,"abstract":"<div><p>Co-pyrolysis behaviors of peanut shells (PS) and tea plant branches (TPB) were explored with a focus on the physicochemical properties, thermal degradation behavior, synergistic effect, and thermo-kinetic analyses. The differences between individual biomass and the equivalent blend were also highlighted. Results showed that the blend sample showed an enhancement in fixed carbon content but a reduction in moisture and ash contents, when compared to those of the individual PS. The average activation energies (<i>E</i>_a) of the equivalent blend estimated by three isoconversional methods (i.e., Friedman, KAS, and Starink methods) were 181.65, 166.87, and 167.14 kJ/mol, respectively. The average <i>E</i>_a and Δ<i>H</i> of the blend were quite lower than those of the TPB but slightly higher than those of the PS. During pyrolysis, Δ<i>H</i> and Δ<i>G</i> exhibited positive values which showed the decomposition was endothermic and non-spontaneous. Negative Δ<i>S</i> values were first observed, followed by positive Δ<i>S</i> values at late conversion stage.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"17 3","pages":"1805 - 1815"},"PeriodicalIF":3.1,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139981166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Pyrolysis Temperature on the Production of Biochar and Biomethanol from Sugarcane Bagasse","authors":"Peter Gabriel Almeida Souza,&nbsp;Jaqueline do Carmo Lima Carvalho,&nbsp;Lorrana Zelia Martins de Souza,&nbsp;Evaneide Nascimento Lima,&nbsp;Mariana Guerra de Aguilar,&nbsp;Robson Pereira Lima,&nbsp;Osania Emerenciano Ferreira,&nbsp;Lúcia Pinheiro Santos Pimenta,&nbsp;Alan Rodrigues Teixeira Machado","doi":"10.1007/s12155-024-10733-8","DOIUrl":"10.1007/s12155-024-10733-8","url":null,"abstract":"<div><p>Biochar is recognized for its potential in mitigating climate change, especially through carbon sequestration and soil improvement. To this end, it is important to use all co-products from pyrolysis in a sustainable and economically viable way. In this study, the conversion of sugarcane bagasse at varying pyrolysis temperatures was investigated using ¹H NMR spectroscopy and Chenomx for liquid fraction analysis. The yield of biochar decreased significantly from 45.3 to 3.5% with a temperature increase of 300 to 1000 °C. The morphological analysis revealed that biochar produced at lower temperatures (300 °C and 400 °C) showed tubular and spongy structures, whereas at higher temperatures (600 °C and 800 °C), the structures morphed into holes and thinned further, ultimately degrading further at 1000 °C. All samples of biochar showed characteristics promising for soil improvement and carbon sequestration (O/C &lt; 0.4). The analysis of liquid fractions revealed that biomethanol reached its highest concentration of 19.28 mM at 800 °C, which coincided with the highest production of acetic and lactic acids. Additionally, the highest concentration of acetone was observed at 600 °C. These findings highlight the importance of optimizing pyrolysis conditions for enhanced yields of biochar and platform compounds, as well as the potential of the NMR and Chenomx in bioenergy research.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"17 3","pages":"1394 - 1401"},"PeriodicalIF":3.1,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139953370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioethanol Production from Alkali-Treated Corn Stover via Acidic Adjustment by Furfural Residue","authors":"Mengxuan Zhang,&nbsp;Yunyun Liu,&nbsp;Wentao Li,&nbsp;Wen Wang,&nbsp;Cuiyi Liang,&nbsp;Yu Zhang,&nbsp;Bao Jia,&nbsp;Wei Qi","doi":"10.1007/s12155-024-10727-6","DOIUrl":"10.1007/s12155-024-10727-6","url":null,"abstract":"<div><p>The unwashed alkali-treated lignocellulose can be directly enzymatically hydrolyzed and fermented via pH adjustment with acids. The use of acids would give a burden on production cost. Furfural residue (FR) which is the acidic solid waste from lignocellulose-derived furfural production process was employed in this study as a pH regulator. The corn cob-derived FR was used to adjust the pH value of alkali-treated corn stover (PCS) to 4.8 for enzymatic hydrolysis and ethanol fermentation. The unwashed PCS adjusted by FR got higher enzymatic hydrolysis efficiency (EHE) than the washed PCS samples. Meanwhile, the mixing of PCS and FR had a synergistic effect on the EHE of PCS. The fermentation of enzymatic hydrolysate from unwashed PCS-FR mixture at 20% solid concentration could attain ethanol production of 26.54 ± 0.02 mg/mL with a yield of 89.53 ± 0.08%. This work created a novel recycling way of FR as a pH regulator for improving the bioconversion of alkali-treated lignocellulose. It also provided a novel clue for the valuable valorization of wastes from corn production.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"17 3","pages":"1386 - 1393"},"PeriodicalIF":3.1,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139927674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethanol Production from Corncob Assisted by Polyethylene Glycol and Conversion of Lignin-Rich Residue into Lignosulfonate and Phenolic Acids","authors":"Julieta Maduzzi,&nbsp;Habila Yusuf Thomas,&nbsp;José Dário Silva Fidelis,&nbsp;José Valderisso Alfredo de Carvalho,&nbsp;Elano Costa Silva,&nbsp;José Daladiê Barreto da Costa Filho,&nbsp;José Demétrio Nery Cavalcante,&nbsp;Everaldo Silvino dos Santos,&nbsp;Domingos Fabiano de Santana Souza,&nbsp;Carlos Eduardo de Araújo Padilha","doi":"10.1007/s12155-024-10725-8","DOIUrl":"10.1007/s12155-024-10725-8","url":null,"abstract":"<div><p>The economic competitiveness of 2G-bioethanol technology should improve through the improvement of the sugar release and the valorization of by-products, especially lignin. Thus, an integrated scheme with corncob was developed to produce ethanol using low dosages of cellulases and value-added products from the semi-simultaneous saccharification and fermentation (SSSF) residue. Enzymatic hydrolysis and SSSF of acid pretreated corncob (&lt; 20 mesh and &gt; 20 mesh) were carried out under cellulase dosages of 5, 10, and 15 FPU/g in the absence and presence of polyethylene glycol 1500 (PEG 1500). The SSSF residue was used to obtain lignosulfonate via sulfomethylation reaction and phenolic acids via alkaline hydrolysis using 4% (w/v) sodium hydroxide and 0–5% (v/v) hydrogen peroxide. Pretreated corncob &lt; 20 mesh allowed the reduction of cellulase dosage to 5 FPU/g without compromising sugar release. The addition of PEG 1500 boosted sugar release, reaching 56.73 g/L glucose under 20% (w/v) solids. The maximum ethanol production of 31.64 g/L was obtained using 5 FPU/g cellulases, 2% (w/w) PEG 1500, and 20% (w/v) solids (gradual addition). FTIR confirmed the preparation of lignosulfonate from SSSF residue, and the surfactant showed good stabilization performance in oil/water systems (emulsification index≈30%). High yields of p-coumaric acid (8045.3 mg/100 g) and ferulic acid (1429.4 mg/100 g) were obtained in alkaline hydrolysis with 5% (v/v) hydrogen peroxide. Based on these findings, corncob is versatile and can create a biorefinery with high economic potential.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"17 3","pages":"1598 - 1611"},"PeriodicalIF":3.1,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139927670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Catalytic Upgrading of Pyrolysis Vapors from Scenedesmus sp. Microalgae towards Renewable Hydrocarbons using a Low-Cost Zeolite Synthesized from Rice Husk Ash and Diatomite Residue","authors":"Júlio de Andrade Oliveira Marques,&nbsp;José Luiz Francisco Alves,&nbsp;Karine Fonseca Soares de Oliveira,&nbsp;Dulce Maria de Araújo Melo,&nbsp;Graco Aurelio Camara de Melo Viana,&nbsp;Renata Martins Braga","doi":"10.1007/s12155-024-10732-9","DOIUrl":"10.1007/s12155-024-10732-9","url":null,"abstract":"<div><p>The present study aims to investigate the potential for producing an aromatic hydrocarbon-enriched fuel from <i>Scenedesmus</i> sp. microalgae using low-cost zeolite in catalytic flash pyrolysis. The methodology adopted in this study involved the use of an analytical micropyrolyzer coupled with a gas chromatograph/mass spectrometer at 500 ºC to assess the effectiveness of the low-cost HZSM − 5 catalyst in deoxygenation, denitrogenation, and aromatization activities of volatile reaction products. The HZSM − 5 catalyst was synthesized using the hydrothermal method, employing low-cost precursor materials, rice husk ash, and diatomite residue as alternative silicon and aluminum sources. The oxygenated and nitrogenated volatile products in non-catalytic flash pyrolysis constituted 51.7% and 15.3%, respectively. Catalytic upgrading of pyrolysis vapors from <i>Scenedesmus</i> sp. microalgae was demonstrated by significant deoxygenation and denitrogenation activity, reaching up to 99%, while chemical industry-relevant classes experienced increased proportions: aromatic hydrocarbons by 5.8-fold, and aliphatic hydrocarbons by 1.7-fold. Around 78% selectivity for aromatic hydrocarbons was achieved, predominantly yielding BTEX (benzene, toluene, ethylbenzene, and xylene). Another significant finding is that 89.8% of the renewable hydrocarbons produced fall within the gasoline range (C₅ − C₁₂). This study conclusively indicates that the low-cost HZSM − 5 catalyst shows significant promise for producing high-quality bio-oil through the flash pyrolysis of <i>Scenedesmus</i> sp. microalgae.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"17 3","pages":"1794 - 1804"},"PeriodicalIF":3.1,"publicationDate":"2024-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139927666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogen and Fatty Acid Production by Dark Fermentation of Sweet Sorghum Stalks as an Efficient Pre-treatment for Energy Recovery Before Their Bioconversion into Methane","authors":"Bakari Hamadou,&nbsp;Djomdi Djomdi,&nbsp;Ruben Zieba Falama,&nbsp;Christine Gardarin,&nbsp;Christelle Blavignac,&nbsp;Fabrice Audonnet,&nbsp;Cedric Delattre,&nbsp;Guillaume Pierre,&nbsp;Pascal Dubessay,&nbsp;Roger Djouldé Darnan,&nbsp;Philippe Michaud,&nbsp;Gwendoline Christophe","doi":"10.1007/s12155-024-10724-9","DOIUrl":"10.1007/s12155-024-10724-9","url":null,"abstract":"<div><p>Hydrogen, volatile fatty acids (VFAs), and methane coproduction from sweet sorghum stems using bacterial consortium was investigated as an efficient and sustainable pre-treatment strategy to improve energy recovery. Integrated two-stage dark fermentation and methanization approach aimed to reduce fractionation, juice extraction, and pre-treatment steps to improve the efficiency and sustainability of stalks energy bioconversion. Stems biomass loading did not significantly influence hydrogen and VFAs productivities. Energy recovery yields were (7.07) and (10.01) MJ/kg dry matter (DM), respectively, for raw stem single dark fermentation (DF) and methanization processes, corresponding to 41.22% and 58.37% of raw stalk energy potential. Methanogenic potential increase of 31.9% and energy bioconversion yield of 13.21 MJ/kg DM were reached for solid residues from DF (80.75% of their energy content), suggesting that bacterial consortium efficiently pre-treated sorghum stalk fibers. Coupling process led to 88.74% net biomass energy recovery yield, corresponding respectively to 57.38% and 40.23% more than single DF and methanization. Fiber degradation ability of DF bacterial consortium significantly contributed to improve sorghum stalk energy recovery efficiency and cost-competitiveness.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"17 3","pages":"1755 - 1769"},"PeriodicalIF":3.1,"publicationDate":"2024-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139680036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biohydrogen production with a degenerated strain of Clostridium acetobutylicum ATCC824 from Eichhornia crassipes biomass","authors":"Paulina Aguirre,&nbsp;Paola German,&nbsp;Karlo Guerrero","doi":"10.1007/s12155-024-10723-w","DOIUrl":"10.1007/s12155-024-10723-w","url":null,"abstract":"<div><p>Degenerate strains of <i>Clostridium acetobutylicum</i> lack the ability to produce solvents and sporulate and remain in a permanent acidogenic state, allowing continuous hydrogen and organic acid production through anaerobic fermentation. <i>Eichhornia crassipes</i>, an invasive aquatic plant, emerges as a promising source of fermentable sugars for hydrogen production via anaerobic fermentation. In this study, a degenerated strain of <i>Clostridium acetobutylicum</i> was isolated and subsequently cultivated in the presence of a hydrolysate solution obtained from the alkaline pre-treatment and enzymatic hydrolysis of <i>Eichhornia crassipes</i>. The hydrolysate was mixed with a defined medium and served the dual purpose of providing essential nutrients and mitigating inhibitors, eliminating the need for an additional detoxification step. A pure defined culture medium served as a control. The extraction methods employed led to the release of low concentrations of inhibitors, reaching 0.1 g/L of furfural and 0.18 g/L of HMF. Kinetic characterization revealed that in the presence of <i>Eichhornia crassipes</i> hydrolysate, the degenerate strain exhibited lower specific growth rates ranging from 0.114 to 0.156 h⁻¹, compared with the control medium which ranged from 0.131 to 0.179 h⁻¹. This was accompanied by lower yields, ranging from 0.115 to 0.167 g_DCW/g in the presence of hydrolysate versus 0.178 to 0.190 g_DCW/g in the control medium, and diminished butyric acid production of 1.318 to 2.932 g/L in the presence of hydrolysate versus 1.749 to 3.471 g/L in control cultures. Despite reduced growth, high biohydrogen volumetric productivity was achieved, reaching 7.3 L/L·d, along with a significant yield of 2.642 mol of hydrogen per mole of glucose consumed. This represents 66.05% of the maximum stoichiometric yield calculated when acetic acid is the sole byproduct. Apparently, the presence of low concentrations of furfural and HMF released during the pre-treatment of <i>Eichhornia crassipes</i> not only negatively affects growth capacity but also diminishes butyric acid production, favoring biohydrogen production.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"17 3","pages":"1770 - 1783"},"PeriodicalIF":3.1,"publicationDate":"2024-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139677625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}