{"title":"The Influence of CO2 on Co-Pyrolysis Characteristics of Sewage Sludge and Poplar Sawdust","authors":"Chen Feng, Xuda Li, Jiaying Xiang, Dongpo Shi, Jiaoyu Zhang, Shenghua Zhu","doi":"10.1007/s12155-026-11013-3","DOIUrl":"10.1007/s12155-026-11013-3","url":null,"abstract":"<div><p>To investigate the influence of CO<sub>2</sub> on the co-pyrolysis characteristics of sewage sludge and poplar sawdust, a 1:1 (w/w) blend of sewage sludge and poplar sawdust was employed as the experimental material. Pyrolysis experiments were performed in a fixed-bed reactor under both CO<sub>2</sub> and N<sub>2</sub> atmospheres across a temperature range of 500–800 ℃. The influence of CO<sub>2</sub> atmosphere on the composition of pyrolysis gas and tar was examined. The results show that increasing temperature decreases the char yield, while increasing the yields of CO, CH<sub>4</sub>, and H<sub>2</sub>. In comparison to N<sub>2</sub>, a CO<sub>2</sub> atmosphere promotes the generation of CH<sub>4</sub> and CO, while suppressing the formation of H<sub>2</sub>. CO<sub>2</sub> atmosphere influence on the composition of tar. The maximum tar yield and the highest total peak area corresponding to phenolic compounds were observed at 500 ℃ under CO<sub>2</sub>, whereas these values were recorded at 600 ℃ under N<sub>2</sub>. Increasing the temperature within CO<sub>2</sub> facilitates the formation of aromatic hydrocarbons in tar. The content of oxygen-containing compounds exhibits an initial decline followed by a subsequent increase.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"19 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147830054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Enhanced Cellulose Isolation from Sugarcane Bagasse through Sequential Alkali and Oxidative Treatment","authors":"Shikha Kumari, Dinesh Arora, Manjeet Kaur, Geeta Dhania","doi":"10.1007/s12155-026-11008-0","DOIUrl":"10.1007/s12155-026-11008-0","url":null,"abstract":"<div><p>As the world seeks sustainable alternatives to fossil-based materials, agricultural residues like sugarcane bagasse are gaining prominence as valuable bioresources. Sugarcane bagasse, the fibrous waste left after juice extraction, is an agricultural residue particularly rich in cellulose, a biopolymer with immense potential for green materials innovation. The present work utilised sugarcane bagasse to obtain cellulose by optimising the concentrations of Sodium hydroxide (NaOH) and Sodium hypochlorite (NaOCl). Characterisation techniques such as Fourier transform infrared spectroscopy (FTIR), field-emission scanning electron microscopy (FE-SEM), and X-ray Diffraction (XRD) were employed for detailed analysis. This article also includes the effect of NaOH and NaOCl concentration on the cellulose yield and the various possible applications of the obtained cellulose. To evaluate statistical significance, a one-way ANOVA was performed, complemented by Tukey’s multiple-comparison test. Compositional analysis showed that the cellulose content of raw sugarcane bagasse was 42.6 ± 1.5%. However, after alkali and bleaching treatment, the cellulose yield ranged from 42.6 ± 0.6% to 56.5 ± 0.5%. FTIR analysis confirmed the successful cellulose extraction from sugarcane bagasse, as evidenced by the disappearance of lignin- and hemicellulose-associated peaks and the appearance of characteristic cellulose absorption bands. XRD analysis revealed an increase in the crystallinity index from 29.8% in SCB to 53.7% in extracted cellulose. Morphological analysis using FE-SEM revealed significant surface differences between SCB and extracted cellulose. Statistical analysis revealed that NaOH and NaOCl amendments significantly increased cellulose yield (p ≤ 0.05) compared with raw sugarcane bagasse. The study highlights the immense potential of agricultural waste as a renewable and cost-effective source of cellulose. By leveraging these residues, industries can reduce their dependence on conventional raw materials while promoting sustainable, environmentally responsible practices.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"19 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147829338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Snehil Rana, Km Diksha, Poonam Gera, Anjireddy Bhavanam
{"title":"Hydrothermal Liquefaction of Deep Eutectic Solvent Treated Alkaline Lignin for Bio-Based Chemicals Production Using Magnetic Biochar Catalyst","authors":"Snehil Rana, Km Diksha, Poonam Gera, Anjireddy Bhavanam","doi":"10.1007/s12155-026-11014-2","DOIUrl":"10.1007/s12155-026-11014-2","url":null,"abstract":"<div><p>The increasing demand for sustainable and eco-friendly alternatives to conventional chemical processes has spurred research into innovative methods for bio-based chemicals production. This study explores the hydrothermal liquefaction (HTL) of alkaline lignin at lower temperatures (sub critical conditions) by treating with Deep Eutectic Solvent (DES) for the production of bio-based chemicals. In this study, a novel magnetic biochar catalyst derived from black liquor was used. The magnetic biochar is incorporated into the HTL process to investigate its catalytic effects on the conversion efficiency of DES-treated alkaline lignin. Catalytic HTL of DES treated alkaline lignin was carried out at temperatures between 140 and 180 ℃ by varying catalysts quantity (3 to 7wt%), solvents (water and ethanol) and reaction times (60–120 min). A maximum bio-oil yield of 57% was obtained at 180 °C with 7 wt% catalyst after 120 min, as both temperature and catalyst loading were increased. Further, the magnetic biochar was characterised with X-ray diffraction (XRD), Fourier-Transform Infrared Spectroscopy (FT- IR), Energy Dispersive X-ray Spectroscopy (EDX) and Field Emission Scanning Electron Microscopy (FESEM). The final product was characterized by Gas Chromatography-Mass Spectroscopy (GC-MS) and 1 H NMR spectroscopy. The results of this research provide valuable insights into the optimization of hydrothermal liquefaction for the production of bio-chemicals from DES-treated alkaline lignin, with a focus on the catalytic potential of magnetic biochar.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"19 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147829092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Suganya, V. Karthik, S. Muralidharan, S. Mariaamalraj
{"title":"Integrated Statistical and Machine-Learning Optimization of Biofuel Extraction from Ulva fasciata Incorporated Zn-Doped CaO Nanoparticle","authors":"R. Suganya, V. Karthik, S. Muralidharan, S. Mariaamalraj","doi":"10.1007/s12155-026-11015-1","DOIUrl":"10.1007/s12155-026-11015-1","url":null,"abstract":"<div><p>Sustainable biofuel production from renewable feedstocks remains a key challenge in the development of low-carbon transportation fuels. This paper outlines an effective approach to biofuel production using marine macroalgal oil produced from <i>Ulva fasciata</i> as a sustainable non-edible feedstock, with focus on environmental suitability and efficiency of the process. The lipids that were obtained after the macroalgal biomass were subjected to proximate and physicochemical analyses to determine the suitability of the feedstock. Zn-doped CaO nano catalyst was developed and systematically characterized to elucidate its structural, morphological and textural properties. Biofuel production was systematically modeled and optimized using response surface methodology (RSM), artificial neural networks (ANN), and a genetic algorithm (GA) to understand process interactions and maximize conversion efficiency. Under optimized conditions, a biofuel yield of 80.78% was achieved, demonstrating effective catalytic performance. Gas chromatography–mass spectrometry analysis confirmed the predominance of C16–C18 fatty acid methyl esters, indicating efficient transesterification. The integration of marine macroalgal biomass, heterogeneous nanocatalyst and hybrid modeling–optimization tools highlight a promising and sustainable route for biofuel production.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"19 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147829339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Clean Hydrogen Fuels from Artocarpus Heterophyllus Peel Waste: Supercritical Water Gasification Enhanced by a Multi-Metal Biochar–Perovskite Catalyst","authors":"Srinivasa Reddy Nallimilli, Renu Sharma, Vinayagam Mohanavel, Rekha M. M, Sathish Kannan, Senthil Kumar Vishnu, Ashwin Jacob, Elumalai Vengadesan, Praveen Barmavatu","doi":"10.1007/s12155-026-11001-7","DOIUrl":"10.1007/s12155-026-11001-7","url":null,"abstract":"<div><p>Food-derived biomass residue, such as jackfruit (Artocarpus heterophyllus) peel, offers a sustainable and abundant feedstock for clean energy production due to its high volatile content and continuous generation. Also, the supercritical water gasification (SCWG) presents an environmentally sound and efficient pathway for converting high-moisture biomass into hydrogen-rich syngas while minimizing solid residues and tar. This study investigates the SCWG performance of jackfruit peel at 300, 450, and 600 °C under 25 MPa for 30 min and evaluates the effect of a multifunctional Mo–Cu@N-biochar–LaFeO<sub>3</sub> catalyst at 5, 10, and 15 wt% loadings. Hydrogen yield increased from 3.45 mmol/g at 300 °C to 9.84 mmol/g at 600 °C, corresponding to enhanced steam reforming and water–gas shift reactions. At 600 °C, gasification efficiency reached 74%, tar formation decreased to 2.4 wt%, and heat recovery achieved 16.3 kJ/g. Catalyst loading significantly strengthened process performance: the 15 wt% formulation enhanced hydrogen yield to 13.5 mmol/g, improved gasification efficiency to 84.5%, reduced tar to 1.7 wt%, and increased heat recovery to 27.1 kJ/g. These results demonstrate the synergistic action of Mo, Cu, N-doped biochar, and LaFeO<sub>3</sub> in promoting clean hydrogen generation, improving energy recovery, and supporting waste-to-energy pathways aligned with sustainable and clean technology objectives.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"19 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147829529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biomass-Derived Biogas, Biochar and Bio-oil: Modification & Upgrading for Industrial Applications","authors":"Sibongiseni Gaqa, Linda Z. Linganiso","doi":"10.1007/s12155-026-11011-5","DOIUrl":"10.1007/s12155-026-11011-5","url":null,"abstract":"<div><p>The world’s energy needs are growing exponentially due to population growth. Both the environment and the world economy are being significantly impacted by the current pattern of energy usage. Research into biomass conversion methods which can produce value-added products has increased due to the global demand for carbon-neutral materials and sustainable energy. In this review article, recent developments in the upgrading and modification of the biomass-derived products for industrial use are reviewed and analysed. Methane upgrading, which is basically carbon dioxide and hydrogen sulfide removal, as well as catalytic activity are the main goals of biogas upgrading to create syngas which is appropriate for chemical synthesis and fuel cells. This study highlights the integration of thermochemical processes with circular bioeconomy principles to maximize energy recovery and lower carbon emissions. The review also investigates that specific upgrading and modification techniques can greatly increase the industrial potential of products derived from biomass, aiding in the shift to greener and more sustainable energy sources.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"19 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12155-026-11011-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147796527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohammad Amin Fazel, Sara Mahdavi, Farid Hosseini, Sahand Azadvar, Omid Tavakoli
{"title":"Comparative Evaluation of Culture Medium and Mixed Culture Effects on Microalgal Biomass, Lipid Productivity, and Biodiesel Quality","authors":"Mohammad Amin Fazel, Sara Mahdavi, Farid Hosseini, Sahand Azadvar, Omid Tavakoli","doi":"10.1007/s12155-026-11010-6","DOIUrl":"10.1007/s12155-026-11010-6","url":null,"abstract":"<div>\u0000 \u0000 <p>Producing microalgal biodiesel with both high lipid productivity and enhanced fuel properties remains an important challenge. The variability in lipid accumulation and biofuel potential among microalgae grown in diverse culture conditions insufficiently characterized. So Addressing this knowledge gap is essential for optimizing microalgal biodiesel to be competitive with traditional fuels. In this study, six microalgal strains and their mixed culture were selected, because they are commonly found, easy to obtain from diverse environments, adaptable for co-cultivation, and possess acceptable lipid content, to evaluate whether species diversity and complementary traits could improve biomass production, lipid accumulation, and biodiesel quality across different cultivation media. The strains and their consortium were cultivated in standard BG-11, bicarbonate-supplemented BG-11, and synthetic municipal wastewater (SMW). This study shows that the mixed culture grown in modified BG-11 have the highest lipid productivity at 35.27 mg L⁻¹ day⁻¹, compared with 33.26 mg L⁻¹ day⁻¹ in standard BG-11 and 16.16 mg L⁻¹ day⁻¹ in SMW. In contrast, nitrogen-limited SMW promoted higher lipid accumulation in some monocultures, while mixed culture improved growth stability and increased the monounsaturated fatty acid content to approximately 50–52% across media. FAME analysis further revealed that modified BG-11 and SMW promoted more favorable saturated and monounsaturated fatty acid profiles, resulting in improved biodiesel properties, with the cetane number (58.8) obtained for the mixed culture in modified BG-11. The findings show that culture medium and mixed-cultivation significantly influence lipid productivity and biodiesel quality in microalgal systems.</p>\u0000 </div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"19 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147797088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alysson Dantas Ferreira, Jéssica Barbosa da Silva do Nascimento, Severino Rodrigues de Farias Neto
{"title":"Influence of Biomass Feed Position on Pyrolysis Performance in a Spouted Bed: A CFD Approach","authors":"Alysson Dantas Ferreira, Jéssica Barbosa da Silva do Nascimento, Severino Rodrigues de Farias Neto","doi":"10.1007/s12155-026-11007-1","DOIUrl":"10.1007/s12155-026-11007-1","url":null,"abstract":"<div>\u0000 \u0000 <p>Brazil is the world’s leading producer of sugarcane, and its bagasse constitutes a substantial biomass resource with a strong potential for renewable energy production via pyrolysis. The development of mathematical models capable of accurately representing pyrolysis phenomena is, therefore, essential for advancing process understanding and optimization. In this study, the influence of biomass particle injection location on pyrolysis conversion rates in a spouted bed reactor was investigated. A CFD Eulerian-Lagrangian model was developed and implemented in OpenFOAM. Model validation involved two preliminary steps: the simulation of single-particle pyrolysis to evaluate reaction kinetics and the simulation of the inert particle bed dynamics. Subsequently, three pyrolysis simulations were performed, varying the biomass injection point: the annular region, the fountain region, and the region above the fountain. Numerical results were validated against experimental data from the literature. Tar and gas production and particle temperature evolution were analyzed for each configuration, all exhibiting characteristic spouted bed behavior. The results demonstrated that biomass injection into the fountain region yielded the highest performance, resulting in a conversion rate of 54.65% and maximizing tar and gas production.</p>\u0000 </div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"19 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12155-026-11007-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147738593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biohydrogen Production Pathways: An Integrated Review of Feedstock Utilization, Technological Developments, Computational Modeling, and Emerging Prospects","authors":"Tesfa Nega Gesese, Desalegn Abera Mersha, Asmare Tezera Admase, Solomon Workneh Fanta, Metadel Kassahun Abera, Atrsaw Jejaw Bezie, Kassaye Gizaw, Gedam Tafere, Ejigayehu Desalegn Asrade, Surafel Argahagn Agdew, Fekadu Mazengiaw Bogale, Kassa Wudineh Liyew, Eshetu Getahun","doi":"10.1007/s12155-026-11006-2","DOIUrl":"10.1007/s12155-026-11006-2","url":null,"abstract":"<div>\u0000 \u0000 <p>Biohydrogen has emerged as a promising renewable energy carrier with significant potential to support global decarbonization and reduce dependence on fossil-derived hydrogen. This review provides a comprehensive and integrated assessment of biohydrogen production pathways, including thermochemical, biochemical, and electrochemical technologies. Major feedstocks such as lignocellulosic biomass, agricultural residues, food waste, industrial wastewater, sewage sludge, algae, and municipal solid waste are critically examined in relation to their availability, composition, pretreatment requirements, and hydrogen production potential. Key conversion pathways, including gasification, pyrolysis, hydrothermal gasification, biogas reforming, dark fermentation, photofermentation, biophotolysis, and microbial electrolysis, are systematically evaluated with respect to reactor design, hydrogen yield, process efficiency, scalability, and technological readiness. Particular emphasis is placed on pretreatment technologies and the trade-off between pretreatment severity and net energy balance, which strongly influence the sustainability and economic viability of lignocellulosic biohydrogen systems. In addition, recent advances in computational modeling, kinetic analysis, metabolic engineering, process simulation, and artificial intelligence are reviewed for their role in improving predictive capability, process optimization, and real-time control. The review further highlights the growing importance of hybrid systems, integrated biorefineries, engineered microbial consortia, advanced materials, and circular bioeconomy strategies in enhancing hydrogen recovery and reducing environmental impacts. Despite notable progress, challenges related to feedstock logistics, low hydrogen yield, process instability, high purification cost, and limited large-scale commercialization remain significant barriers. Overall, this review provides a forward-looking roadmap for the development of sustainable, efficient, and commercially viable biohydrogen production systems.</p>\u0000 </div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"19 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147738922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fernando Roberto Paz Cedeno, Gustavo R. Gomes, Jessica J. Silva, Fabricia F. Menezes, Viviane M. Nascimento, George J. M. Rocha, Renata P. Rodriguez, Xiaowen Chen, Antonio Bonomi, Carlos Driemeier
{"title":"Deacetylation and Mechanical Refining Pathway for the Bioconversion of Sugarcane Bagasse","authors":"Fernando Roberto Paz Cedeno, Gustavo R. Gomes, Jessica J. Silva, Fabricia F. Menezes, Viviane M. Nascimento, George J. M. Rocha, Renata P. Rodriguez, Xiaowen Chen, Antonio Bonomi, Carlos Driemeier","doi":"10.1007/s12155-026-10994-5","DOIUrl":"10.1007/s12155-026-10994-5","url":null,"abstract":"<div>\u0000 \u0000 <p>Advancing lignocellulose biorefining is imperative for the deployment of cellulosic (2G) biofuels. This work investigates the tailoring of the alkaline deacetylation and mechanical refining (DMR) pathway for the bioconversion of sugarcane bagasse. Experiments are conducted at laboratory and pilot scales, varying the pretreatment conditions (70–92 °C; 48–100 g<sub>NaOH</sub>/kg) and the mechanical refining technologies (PFI and disk refining). The pretreatments selectively solubilize acetyl groups (> 86%) and lignin (10–63%) while mostly preserving structural carbohydrates in the solid phase. Enzymatic hydrolysis generates hydrolysates of clean sugars (glucose and xylose), with sugar yields increasing up to 81% for glucose and 89% for xylose in response to delignification and mechanical refining. Biochemical methane potential assays reveal specific methane productions of up to 568 NmL CH₄ gVS⁻¹ for alkaline liquor monodigestion and 344 NmL CH₄ gVS⁻¹ for co-digestion with sugarcane vinasse from the conventional (1G) sugarcane ethanol, indicating a strong potential for bioenergy recovery from this process stream. Synergies are identified in integrating 1G ethanol, 2G<sub>DMR</sub> processing of bagasse, and anaerobic co-digestion of 1G vinasse and 2G<sub>DMR</sub> alkaline liquor. This technology enables sugarcane biorefineries to enhance the co-production of ethanol, methane, and concentrated streams of CO<sub>2</sub>.</p>\u0000 </div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"19 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12155-026-10994-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147738765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}