Canadian Journal of Gastroenterology and Hepatology最新文献

{"title":"Pathological Characteristics, Prognostic Determinants and the Outcome of Patients Diagnosed with Colorectal Adenocarcinoma at the University Teaching Hospital of Kigali.","authors":"Delphine Uwamariya, Déogratias Ruhangaza, Belson Rugwizangoga","doi":"10.1155/2022/6608870","DOIUrl":"10.1155/2022/6608870","url":null,"abstract":"<p><p>Worldwide, colorectal cancer (CRC) is the second most diagnosed cancer in female and the third in men, arising from the epithelium of the colorectum. It is known that colorectal cancer is common in developed countries than in developing countries which may be due to inaccurate data on the existence of the disease in that region combined with embracing western lifestyle expressed by the current trend of changes in cultural, social, and lifestyle practices playing a major part in the etiology of CRC. The aim of this study was to document epidemiological, pathological characteristics, and prognostics determinants of patients diagnosed with CRC in Rwanda. The data from patients' files and reviewed glass slides for 101 cases all from Kigali University Teaching Hospital (CHUK) were statistically analyzed and patient characteristics were described as mean and frequency accordingly. Comparisons were performed using chi square tests, Fisher's exact test and odds ratio with 95% confidence interval (CI). Survival curves were plotted using the Kaplan-Meier method, and log-rank test was used to assess the statistical differences in the observed survival curves by each categorical variable. A <i>P</i> value < 0.05 was considered statistically significant. Statistical analyses were performed using Statistical Product and Service Solutions (SPSS), GraphPad Prism, and MedCalc, accordingly. Mean age of the participants was 54.26 years, the main symptom was rectal bleeding (46.5%), rectal adenocarcinoma NOS represented 40.6%, conventional adenocarcinoma was 60.4%, most tumors were of Grade II (54.5%), most common stage was pT3N0 (20.8%), resection margins were free at 71.3%, lympho-vascular invasion was 49.5% of cases, a high immune response was in 71.3% of cases and of 101cases, and 55.4% were still alive at the end of the data collection, with 29.3% of patients have overall survival of 5 years. Prognostic determinants also affect the outcome in this study and overall survival period was 3 years for CRC diagnosed in Rwanda.</p>","PeriodicalId":48755,"journal":{"name":"Canadian Journal of Gastroenterology and Hepatology","volume":" ","pages":"6608870"},"PeriodicalIF":2.7,"publicationDate":"2022-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9553703/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33515096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Portosystemic Shunt for the Control of Variceal Bleeding in Cirrhotic Patients: Past and Present.","authors":"Petre Radu,&nbsp;Virgiliu-Mihail Prunoiu,&nbsp;Victor Strâmbu,&nbsp;Dragos Garofil,&nbsp;Roxana Elena Doncu,&nbsp;Eugen Brătucu,&nbsp;Laurentiu Simion,&nbsp;Maria-Manuela Răvaş,&nbsp;Mircea Nicolae Brătucu","doi":"10.1155/2022/1382556","DOIUrl":"https://doi.org/10.1155/2022/1382556","url":null,"abstract":"<p><p>Based on an experience of more than 50 years in the treatment of portal hypertension (PHT), the authors review and analyze the evolution of the surgical portocaval shunt (PCS). We would like to provide an insight into the past of PCS, in order to compare it with the current state of the treatment of PHT complications. As a landmark of the past, we shall present statistics of more than 500 cases of PHT operated between 1968 and 1983. From this group, 238 patients underwent surgical portocaval shunting during a fifteen-year period. The behavior of the portal hemodynamics following PCS was studied and the postoperative decrease in portal pressure (PP), as well as the residual PP, were recorded. The portal manometric determinations were made by electronic recordings using the Hellige device and direct intraoperative recordings through the catheterization of a ramus in the portal area. The results of PCS are superposable, in terms of hemodynamic efficiency, with those of the intrahepatic shunt (TIPS-transjugular intrahepatic portosystemic shunt). The authors discuss the current place of PCS, in obvious decline in comparison with the situation 50 years ago. The current methods of controlling variceal bleeding represent obvious progress. PCS remains with very limited indications, in specific situations when the other therapeutic methods have failed or are not recommended.</p>","PeriodicalId":48755,"journal":{"name":"Canadian Journal of Gastroenterology and Hepatology","volume":" ","pages":"1382556"},"PeriodicalIF":2.7,"publicationDate":"2022-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9509272/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40376298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recipient Age Predicts 20-Year Survival in Pediatric Liver Transplant.","authors":"Stephanie Keeling,&nbsp;Malcolm F McDonald,&nbsp;Adrish Anand,&nbsp;Jordan Dunson,&nbsp;Elizabeth Williams,&nbsp;Theodore Zhang,&nbsp;Brian Hickner,&nbsp;Nhu Thao Nguyen Galván,&nbsp;Christine O' Mahony,&nbsp;John A Goss,&nbsp;Abbas Rana","doi":"10.1155/2022/1466602","DOIUrl":"https://doi.org/10.1155/2022/1466602","url":null,"abstract":"<p><strong>Introduction: </strong>Pediatric liver transplant recipients have demonstrated excellent long-term survival. The purpose of this analysis is to investigate factors associated with 20-year survival to identify areas for improvement in patient care.</p><p><strong>Methods: </strong>Kaplan-Meier with log-rank test as well as univariate and multivariate logistic regression methods were used to retrospectively analyze 4,312 liver transplant recipients under the age of 18 between September 30, 1987 and March 9, 1998. Our primary endpoint was 20-year survival among one-year survival.</p><p><strong>Results: </strong>Logistic regression analysis identified recipient age as a significant risk factor, with recipients below 5 years old having a higher 20-year survival rate (<i>p</i> < 0.001). A preoperative primary diagnosis of a metabolic dysfunction was found to be protective compared to other diagnoses (OR 1.64, CI 1.20-2.25). African-American ethnicity (OR 0.71, CI 0.58-0.87) was also found to be a risk factor for mortality. Technical variant allografts (neither living donor nor cadaveric) were not associated with increased or decreased rates of 20-year survival.</p><p><strong>Conclusions: </strong>Our analysis suggests that long-term survival is inversely correlated with recipient age following pediatric liver transplant. If validated with further studies, this conclusion may have profound implications on the timing of pediatric liver transplantation.</p>","PeriodicalId":48755,"journal":{"name":"Canadian Journal of Gastroenterology and Hepatology","volume":" ","pages":"1466602"},"PeriodicalIF":2.7,"publicationDate":"2022-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9509270/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40376299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Prognostic Value of Serum HBV-RNA during Hepatitis B Virus Infection is Related to Acute-on-Chronic Liver Failure.","authors":"Keli Qian,&nbsp;Ying Xue,&nbsp;Hang Sun,&nbsp;Ting Lu,&nbsp;Yixuan Wang,&nbsp;Xiaofeng Shi","doi":"10.1155/2022/8422242","DOIUrl":"https://doi.org/10.1155/2022/8422242","url":null,"abstract":"<p><strong>Background: </strong>Serum HBV-RNA levels can predict antiviral response in chronic hepatitis B (CHB) patients; however, its role in HBV-related ACLF (HBV-ACLF) remains unclear. Here, we determined its implications for HBV-ACLF.</p><p><strong>Methods: </strong>Baseline serum HBV-RNA levels were retrospectively detected in HBV-ACLF and CHB patients. The association of serum HBV-RNA level with clinical outcomes was evaluated by performing multiple logistic regression. A nomogram was developed to formulate an algorithm incorporating serum HBV-RNA for predicting the survival of HBV-ACLF patients. After being discharged from the hospital, the HBV-ACLF patients were followed up for 36 weeks.</p><p><strong>Results: </strong>In this study, 82 HBV-ACLF patients and 33 CHB patients were included. Serum HBV-RNA levels were significantly higher in CHB patients than in HBV-ACLF patients (4.15 ± 2.63 log10 copies/mL VS 5.37 ± 2.02 log10 copies/mL) (<i>P</i> < 0.05). Among the HBV-ACLF cases, patients with poor outcomes had lower serum HBV-RNA levels, but the difference was not significant. The area under the receiver operating characteristic curve of the serum HBV-RNA inclusive model was 0.745, superior to 0.66 from MELD scores (<i>P</i> < 0.05). During the follow-up for four weeks, the serum HBV-RNA levels, especially in the survival group, were found to be lower than the baseline levels.</p><p><strong>Conclusions: </strong>Serum HBV-RNA levels were associated with disease severity and might predict the long-term clinical outcome of HBV-ACLF patients.</p>","PeriodicalId":48755,"journal":{"name":"Canadian Journal of Gastroenterology and Hepatology","volume":" ","pages":"8422242"},"PeriodicalIF":2.7,"publicationDate":"2022-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9489391/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33479271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Misconceptions Drive COVID-19 Vaccine Hesistancy in Individuals with Inflammatory Bowel Disease.","authors":"Eva Zhang,&nbsp;Arun Gupta,&nbsp;Aysha Al-Ani,&nbsp;Finlay A Macrae,&nbsp;Rupert W Leong,&nbsp;Britt Christensen","doi":"10.1155/2022/4527844","DOIUrl":"https://doi.org/10.1155/2022/4527844","url":null,"abstract":"<p><strong>Background: </strong>Vaccination is an effective public health measure to combat the SARS-CoV-2 pandemic. However, vaccine \"hesitancy\" has limited uptake in some, including inflammatory bowel disease (IBD) patients who may have unique concerns influencing uptake.</p><p><strong>Aim: </strong>The aim of the study is to explore attitudes, concerns, and the influence of different sources of information on COVID-19 vaccine uptake in IBD patients.</p><p><strong>Methods: </strong>Patients from a specialist IBD clinic at a tertiary hospital in Australia and a national IBD patient society were invited to complete an anonymous online survey regarding COVID-19 vaccination. Demographic characteristics, attitudes towards vaccination, and trust in sources of information were explored. Logistic regression was used to identify variables associated with vaccine uptake.</p><p><strong>Results: </strong>Of 441 respondents, 93% of respondents had received at least 1 dose of COVID-19 vaccination. Self-perceived risk of being more unwell with COVID-19 infection due to IBD (AOR 5.25, 95% CI 1.96-14.04, <i>p</i> < 0.001) was positively associated with vaccine uptake. Concerns regarding the safety of vaccination in pregnancy (OR 0.22, 95% CI 0.08-0.65, <i>p</i>=0.006) and of causing an IBD flare (OR 0.28, 95% CI 0.10-0.77, <i>p</i>=0.01) were negatively associated with vaccine uptake. In total, 282 (73.7%) responders ranked healthcare workers the most trusted source to obtain information surrounding vaccination.</p><p><strong>Conclusion: </strong>Vaccine hesitancy in IBD patients is low. Concerns about the safety of vaccination in pregnancy and in causing an IBD flare are both associated with vaccine hesitancy. Healthcare providers play a key role in proactively addressing these misconceptions particularly in the context of emerging virus variants and the availability of boosters.</p>","PeriodicalId":48755,"journal":{"name":"Canadian Journal of Gastroenterology and Hepatology","volume":" ","pages":"4527844"},"PeriodicalIF":2.7,"publicationDate":"2022-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9482530/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40371223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyethylene Glycol 3350 in the Treatment of Chronic Idiopathic Constipation: Post hoc Analysis Using FDA Endpoints.","authors":"Stacy B Menees,&nbsp;Anthony J Lembo,&nbsp;William D Chey","doi":"10.1155/2022/3533504","DOIUrl":"https://doi.org/10.1155/2022/3533504","url":null,"abstract":"<p><strong>Methods: </strong>This multicenter, double-blind, placebo-controlled, parallel-group trial included adults with chronic idiopathic constipation randomized to polyethylene glycol 3350 17 g (<i>n</i> = 204) or placebo (<i>n</i> = 100) once daily for 24 weeks. Post hoc analyses were performed using the US Food and Drug Administration endpoint (≥3 complete spontaneous bowel movements/week and an increase of ≥1 complete spontaneous bowel movement/week from baseline for ≥9/12 weeks, including 3 of the last 4 weeks) along with additional efficacy and safety outcomes.</p><p><strong>Results: </strong>The proportion of patients meeting the new endpoint was significantly higher with polyethylene glycol 3350 vs placebo (42% vs 13%; <i>P</i> < 0.0001). Reductions in the mean number of hard/lumpy stools/week (-2.1 vs -0.9; <i>P</i> = 0.0014) and the weekly mean five-point cramping rating (-0.3 vs -0.1; <i>P</i> = 0.0272) also significantly favored polyethylene glycol 3350. The proportion of subjects with gastrointestinal adverse events decreased markedly after the first week of treatment in the polyethylene glycol 3350 group.</p><p><strong>Conclusion: </strong>Using the current US Food and Drug Administration-recommended responder definition and other secondary outcomes, once-daily polyethylene glycol 3350 demonstrated substantial and sustained efficacy and safety over 24 weeks in patients with chronic idiopathic constipation. <i>Trial Registration.</i> The original trial was registered with https://clinicaltrials.gov Trial: NCT00153153.</p>","PeriodicalId":48755,"journal":{"name":"Canadian Journal of Gastroenterology and Hepatology","volume":" ","pages":"3533504"},"PeriodicalIF":2.7,"publicationDate":"2022-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9481403/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40367647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of an Abdominal Compression Bandage on the Completion of Colonoscopy for Obese Adults: A Prospective Randomized Controlled Trial.","authors":"Ting-Ting Liu,&nbsp;Yi-Teng Meng,&nbsp;Feng Xiong,&nbsp;Cheng Wei,&nbsp;Su Luo,&nbsp;Sheng-Gang Zhan,&nbsp;Yang Song,&nbsp;Ying-Xue Li,&nbsp;Rui-Yue Shi,&nbsp;Jun Yao,&nbsp;Li-Sheng Wang,&nbsp;De-Feng Li","doi":"10.1155/2022/6010367","DOIUrl":"https://doi.org/10.1155/2022/6010367","url":null,"abstract":"<p><strong>Methods: </strong>Eligible patients were randomly allocated into the abdominal bandage and conventional groups during a routine colonoscopy. The primary outcome was CCR.</p><p><strong>Results: </strong>A total of 250 eligible patients were randomly assigned to the abdominal bandage and conventional groups from January 2021 to April 2021. Eleven patients (five in the abdominal bandage group and six in the conventional group) were excluded due to schedule cancellation after randomization, and 239 patients were eventually included in the final analysis. There were no significant differences between the two groups regarding baseline characteristics (<i>P</i> > 0.05). Furthermore, no significant differences were observed in terms of advanced adenoma detection rate (AADR), polyp detection rate (PDR), bowel preparation scale (BBPS), bubble scale (BS), and withdrawal time between the two groups (<i>P</i> > 0.05). However, compared with the conventional group, the cecal insertion time (CIT) of the abdominal bandage group was significantly shortened (279.00 (234.50-305.75) vs. 421.00 (327.00-485.00), <i>P</i> < 0.001), and the CCR (96.7% vs. 88.2%, <i>P</i> = 0.01) and adenoma detection rate (ADR) (47.5% vs. 32.8%, <i>P</i> < 0.001) were improved. Besides, logistic regression analysis showed that body mass index (BMI) and abdominal compression bandage were associated with CCR.</p><p><strong>Conclusions: </strong>Abdominal compression bandages could effectively shorten CIT and improve CCR and ADR for obese patients during a routine colonoscopy. This trial is registered with the Chinese Clinical Trial Registry (No. ChiCTR2100043556).</p>","PeriodicalId":48755,"journal":{"name":"Canadian Journal of Gastroenterology and Hepatology","volume":" ","pages":"6010367"},"PeriodicalIF":2.7,"publicationDate":"2022-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9470372/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40361188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and Tolerability of Two Different Low-Volume Split-Dose Polyethylene Glycol Electrolytes Solution Bowel Preparation for Morning Colonoscopy.","authors":"Hefeng Tian,&nbsp;Hui Li,&nbsp;Xuanrui Zhu,&nbsp;Wenlong Liu,&nbsp;Ying Fan,&nbsp;Lei Shi,&nbsp;Xiu Wang","doi":"10.1155/2022/8169649","DOIUrl":"https://doi.org/10.1155/2022/8169649","url":null,"abstract":"<p><strong>Methods: </strong>A total of 120 patients were randomized to receive either the control group (<i>n</i> = 64) or the experimental group (<i>n</i> = 65). Patients in the control group adopted the low-volume split-dose regimen one, and patients in the experimental group adopted the low-volume split-dose regimen two. Those randomized to regimen one were instructed to take 0.75 L PEG two hours after dinner the day before the colonoscopy and 1.5 L PEG 4 hours before the colonoscopy. Patients assigned to regimen two were invited to consume 1.5 L PEG two hours after dinner the day before the colonoscopy and 0.75 L PEG 4 hours before the colonoscopy. The quality of bowel preparation, rated according to a Boston Bowel Preparation Scale (BBPS), represented the primary outcome measure. Tolerability, satisfaction, and lesions detection rated were secondary outcomes.</p><p><strong>Results: </strong>There was no significant difference between the transverse colon and right colon scores between the two groups (<i>P</i> > 0.05). The low-volume split-dose regimen two showed a higher success rate for cleansing of the right colon and overall colon (<i>P</i> < 0.05). For the comparison of the patients' bowel tolerance, there were no statistical differences between the two groups regarding thirst, abdominal pain or abdominal discomfort, abdominal distension, dizziness or headache, anal discomfort, and sleep disturbance (<i>P</i> > 0.05). However, regimen two had significantly less nausea, vomiting, and fatigue than regimen one (24.62% vs. 42.19%, <i>P</i>=0.034; 10.77% vs. 25.00%, <i>P</i>=0.035; 6.15% vs. 21.88%, <i>P</i>=0.010, respectively). Patient-reported satisfaction and willingness to repeat the bowel preparation were significantly higher for low-volume split-dose regimen two than for low-volume split-dose regimen one (<i>P</i>=0.011; <i>P</i>=0.015).</p><p><strong>Conclusions: </strong>In early morning colonoscopies, the bowel-cleansing efficacy and patient tolerability of low-volume split-dose regimen two were superior to low-volume split-dose regimen one.</p>","PeriodicalId":48755,"journal":{"name":"Canadian Journal of Gastroenterology and Hepatology","volume":" ","pages":"8169649"},"PeriodicalIF":2.7,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9453094/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33460944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>ptk2</i> and <i>mt2a</i> Genes Expression in Gastritis and Gastric Cancer Patients with <i>Helicobacter pylori</i> Infection.","authors":"Manouchehr Ahmadi Hedayati,&nbsp;Delniya Khani,&nbsp;Farshad Sheikhesmaeili,&nbsp;Bijan Nouri","doi":"10.1155/2022/8699408","DOIUrl":"https://doi.org/10.1155/2022/8699408","url":null,"abstract":"<p><strong>Background: </strong><i>ptk2</i> and <i>mt2a</i> genes contribute to the cell cycle during proliferation and apoptosis, respectively. Designing a case-control study including gastric adenocarcinoma and gastritis patients with and without <i>Helicobacter pylori</i> infection would lead to determinate of the correlations between <i>ptk2</i> and <i>mt2a</i> genes expression with <i>H. pylori</i> infection in gastric antral epithelial cells.</p><p><strong>Methods: </strong>Overall, 50 and 30 gastric antral biopsy samples of gastric cancer (case group) and gastritis (control group) patients were included into study, respectively. All biopsy samples were collected considering the exclusion criteria including patients with a history of consumption of tobacco, alcohol, and anti-<i>H. pylori</i> drugs. Each patient group is divided into with and without <i>H. pylori</i> infection to detect cDNA fold changes of <i>ptk2</i> and <i>mt2a</i> genes by using Real Time RT PCR. Furthermore, the presence of <i>H. pylori</i> virulence genes was detected directly by using specific primers and simple PCR on cDNA synthesized from total RNA of gastric antral biopsy samples.</p><p><strong>Results: </strong>A negative correlation was revealed between age and clinical manifestations with the ΔCt value of the <i>ptk2</i> gene (<i>P</i> < 0.05). The <i>H. pylori iceA1/2</i> and <i>cagE</i> genes revealed positive and negative correlations with the ΔCt value of the <i>ptk2</i> gene (<i>P</i> < 0.05), respectively. Furthermore, a weak correlation was detectable between <i>H. pylori babA2/B, oipA</i>, and <i>cagY</i> genes and the ΔCt value of the <i>mt2a</i> gene in gastric antral epithelial cells of patients (<i>P</i> < 0.1).</p><p><strong>Conclusions: </strong>The results of the current study opened a view for more investigation on the stunning roles of <i>H. pylori</i> infection in clinical outcomes through <i>mt2a</i> and <i>ptk2</i> gene expression in gastric antral epithelial cells.</p>","PeriodicalId":48755,"journal":{"name":"Canadian Journal of Gastroenterology and Hepatology","volume":" ","pages":"8699408"},"PeriodicalIF":2.7,"publicationDate":"2022-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9436627/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40346738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Characteristics and Potential Mechanisms in Patients with Abnormal Liver Function Indices and Elevated Serum IgG4.","authors":"Jing Wang,&nbsp;Yue Zhang,&nbsp;Dandan Jiang,&nbsp;Lu Zhou,&nbsp;Bangmao Wang","doi":"10.1155/2022/7194826","DOIUrl":"https://doi.org/10.1155/2022/7194826","url":null,"abstract":"<p><strong>Objective: </strong>We analyzed the etiological classification and clinical characteristics of patients with abnormal liver function indices and elevated serum IgG4 levels and investigated the effects of intrahepatic follicular helper T cell (Tfh) infiltration and serum IL-21.</p><p><strong>Methods: </strong>Clinical data (age, sex, past history, clinical manifestations, laboratory tests, imaging, diagnosis, and treatment) and etiology of liver injury from 136 patients were analyzed. We compared the general condition, clinical characteristics, and laboratory tests of 19 AIH (autoimmune hepatitis) patients with elevated serum IgG4 levels with those of 20 AIH patients with normal serum IgG4 levels admitted at the same time. Five patients with AIH and elevated serum IgG4 levels and five AIH patients with normal IgG4 levels were matched by sex, age, and liver function, and Tfh infiltration in liver biopsy tissues of patients in both groups was determined by immunofluorescence staining. Five AIH patients with elevated serum IgG4 levels were selected for measurement of serum interleukin-21 (IL-21) levels by enzyme-linked immunosorbent assay (ELISA), seventeen AIH patients with normal serum IgG4 were matched by sex, age, and liver function indices, and 29 physically healthy individuals matched by sex and age were selected as the control group. The changes in patients with IgG4-RD and abnormal liver function before and after glucocorticoid treatment were measured.</p><p><strong>Results: </strong>Patients (136) with abnormal liver function indices and elevated serum IgG4 levels were diagnosed with liver disease of different etiologies. IgG4-related disease was the most frequent, followed by AIH and malignancy. Abnormal liver function indices with high serum IgG4 were most commonly seen as elevated gamma glutamyl transferase (GGT). The AIH group with elevated serum IgG4 had increased intrahepatic levels of Tfh. IL-21 in AIH patients with elevated IgG4 was higher than in patients with normal IgG4 and healthy controls. Patients (<i>n</i> = 28) with abnormal liver function indices and IgG4-related disease received glucocorticoid therapy for six months, and ALT, AST, ALKP, GGT, TBil, DBil, IgG, IgG4, and IgE were significantly lower after treatment.</p><p><strong>Conclusions: </strong>Elevated serum IgG4 was seen in patients with abnormal liver function indices with diverse causes. Tfh infiltration and increased IL-21 production may be related to the pathogenesis of AIH with elevated serum IgG4. Glucocorticoid therapy is effective in patients with abnormal liver function indices and IgG4-related disease. Assessing immune function in patients with abnormal liver function indices and elevated serum IgG4 levels should facilitate diagnosis and treatment of the disease.</p>","PeriodicalId":48755,"journal":{"name":"Canadian Journal of Gastroenterology and Hepatology","volume":" ","pages":"7194826"},"PeriodicalIF":2.7,"publicationDate":"2022-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9436615/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40346740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}