Canadian Journal of Gastroenterology and Hepatology最新文献

{"title":"Antiviral Therapy for a Postpartum Flare in Women with Chronic HBV Infection Shortens the ALT Recovery Time and Reduces Hepatitis Re-Flare Rates within 4 years.","authors":"Min Quan, Cong Liu, Wei Li, Hui-Chun Xing","doi":"10.1155/2022/4753267","DOIUrl":"10.1155/2022/4753267","url":null,"abstract":"<p><strong>Background: </strong>Few studies explored whether anti-hepatitis B virus (HBV) therapy should be initiated during postpartum hepatitis flare.</p><p><strong>Aim: </strong>This study aimed to analyze the effect of anti-HBV therapy on postpartum hepatitis flare and evaluate the prognosis within 4 years postpartum.</p><p><strong>Methods: </strong>This retrospective study enrolled hepatitis B surface antigen (HBsAg)-positive and hepatitis B e antigen (HBeAg)-positive pregnant women with HBV DNA ≥ 10⁶ IU/mL. A total of 152 pregnant women were included: 103 in the prophylactic anti-HBV therapy group (PT-G) and 49 in the non-prophylactic anti-HBV therapy group (NPT-G). The women with a postpartum flare were assigned to the anti-HBV therapy group (AT-G) and non-anti-HBV therapy group (NAT-G) to analyze the effect of postpartum anti-HBV therapy on hepatitis flare. Virological and biochemical parameters were assessed.</p><p><strong>Results: </strong>Taking postpartum 12 weeks as the cutoff point, the ALT recovered time for postpartum flare women is shorter in AT-G (<i>n</i> = 16, 42.1%) or PT-G (<i>n</i> = 23, 34.8%) than in NAT-G (<i>n</i> = 14, 23.0%; <i>x</i> ² = 4.067, <i>P</i>=0.044) or NPT-G (<i>n</i> = 4, 11.1%; <i>x</i> ² = 5.579, <i>P</i>=0.018). Taking postpartum 26 weeks as the cutoff point, the ALT recovered time is shorter in AT-G (<i>n</i> = 35, 57.3%) or PT-G (<i>n</i> = 44, 66.7%) than in NAT-G (<i>n</i> = 32, 84.2%; <i>x</i> ² = 7.707, <i>P</i>=0.006) or NPT-G (<i>n</i> = 16, 44.4%; <i>x</i> ² = 4.749, <i>P</i>=0.029). Postpartum flare recovery time was positively correlated with HBV DNA level at delivery [<i>r</i> = 0.223, <i>P</i>=0.025, 95%CI (0.022～0.41)]. The hepatitis re-flare rates within postpartum 4 years in AT-G (<i>n</i> = 3, 9.68%) is lower than that in NAT-G (<i>n</i> = 24, 45.4%; <i>x</i> ² = 14.003, <i>P</i> ≤ 0.001). The HBeAg, HBsAg, HBV DNA, and ALT level at postpartum 4 years in AT-G were lower than that in NAT-G (<i>P</i> < 0.001).</p><p><strong>Conclusion: </strong>Anti-HBV therapy for postpartum hepatitis flare of women with chronic HBV could shorten the ALT recovery time and reduce hepatitis re-flare rates within 4 years of postpartum.</p>","PeriodicalId":48755,"journal":{"name":"Canadian Journal of Gastroenterology and Hepatology","volume":" ","pages":"4753267"},"PeriodicalIF":2.7,"publicationDate":"2022-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9236834/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40406416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Modified Glasgow Prognostic Score Predicts Survival in Gastric Cancer Patients with Normal CEA and CA19-9.","authors":"Shun Zhang,&nbsp;Jing-Ze Li,&nbsp;Tao Du,&nbsp;Hai-Qiang Li,&nbsp;Ren-Hao Hu,&nbsp;Chi-Ye Ma,&nbsp;Xi-Mao Cui,&nbsp;Chun Song,&nbsp;Xiao-Hua Jiang","doi":"10.1155/2022/3953004","DOIUrl":"https://doi.org/10.1155/2022/3953004","url":null,"abstract":"<p><strong>Background: </strong>Traditionally, serum CEA and CA19-9 levels are good prognostic factors for gastric cancer. Many gastric cancer patients do not have elevated CEA or CA19-9 levels even at a very advanced stage. This study investigates the significance of the modified Glasgow prognostic score (mGPS) for the survival of gastric cancer patients with normal CEA and CA19-9.</p><p><strong>Methods: </strong>We retrospectively examined 488 curatively resected gastric cancer patients with normal preoperative serum levels of CEA and CA19-9 to evaluate the prognostic ability of mGPS for overall survival. The prognostic significance was analyzed by univariate and multivariate analyses.</p><p><strong>Results: </strong>Age, hemoglobin, white cell count, and neutrophils were each significantly correlated with the mGPS. Multivariate analyses showed that tumor location (HR, 0.803; 95% CI, 0.667-0.966; <i>P</i>=0.020), TNM stage (HR, 2.714; 95% CI, 2.250-3.275; <i>P</i> < 0.001), and mGPS (HR, 1.042; 95% CI, 1.105-1.772; <i>P</i>=0.023) were significantly associated with overall survival. Significant correlations were found between overall survival and mGPS. The Kaplan-Meier analysis demonstrated significant differences among patients with mGPS of 0, 1, and 2 (<i>P</i> < 0.001), with the mortality rate being higher for patients with a higher mGPS.</p><p><strong>Conclusion: </strong>The mGPS can predict survival in gastric cancer patients with normal CEA and CA19-9.</p>","PeriodicalId":48755,"journal":{"name":"Canadian Journal of Gastroenterology and Hepatology","volume":" ","pages":"3953004"},"PeriodicalIF":2.7,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208994/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40225037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Half-Transected and Self-Pulling Esophagojejunostomy in Total Laparoscopic Gastrectomy for Gastric Cancer: A Safe and Feasible Technique.","authors":"Hongtao Wan,&nbsp;Jianyong Xiong,&nbsp;Yanglin Chen,&nbsp;Haiyun Wei,&nbsp;Ren Tang,&nbsp;Chao Chen,&nbsp;Qing Sun,&nbsp;Jing Xu,&nbsp;Bo Yi","doi":"10.1155/2022/2422274","DOIUrl":"https://doi.org/10.1155/2022/2422274","url":null,"abstract":"<p><strong>Objective: </strong>This study introduces a technique for esophagojejunostomy with half transected and self-pulling (HTSP) and evaluates the safety, feasibility, and clinical results of this technique in totally laparoscopic total gastrectomy (TLTG).</p><p><strong>Materials and methods: </strong>From May 2019 to March 2021, 42 patients (HTSP group) who underwent HTSP-TLTG surgery in the Department of Abdominal Tumor Surgery of Jiangxi Cancer Hospital were included in this study. The control group consisted of 50 patients undergoing conventional TLTG surgery (conventional anastomosis group) performed by the same surgical team from March 2018 to March 2020. The clinical data of the two groups were retrospectively analyzed and compared.</p><p><strong>Results: </strong>The mean operation time of the HTSP-TLTG surgery was 166.7 ± 13.1 minutes and the anastomosis time was 20.8 ± 2.0 minutes, which were significantly shorter than those of traditional TLTG (<i>P</i> < 0.05). There were no significant differences between the two groups in blood loss, time to first exhaust, postoperative hospital stay, and incidence of surgery-related complications.</p><p><strong>Conclusion: </strong>HTSP is a safe and feasible way of endoscopic esophagojejunal anastomosis, which requires a relatively low suture technique under endoscopy, and is suitable for promotion.</p>","PeriodicalId":48755,"journal":{"name":"Canadian Journal of Gastroenterology and Hepatology","volume":" ","pages":"2422274"},"PeriodicalIF":2.7,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208976/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40225038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Albumin Paclitaxel Compared with 5-Penfluorouracil, Lobaplatin, and Albumin Paclitaxel Combined with 5-Penfluorouracil in the Treatment of Human Gastric Cancer Cell AGS Line Autophagy and Apoptosis.","authors":"Xingzhen Cheng,&nbsp;Fang Yang,&nbsp;Yang Wang,&nbsp;Wei Nie,&nbsp;Adarsha Mahendra Upadhyay,&nbsp;Maolin Zhang,&nbsp;Qian Wang,&nbsp;Zhiqiang Yan","doi":"10.1155/2022/6015877","DOIUrl":"https://doi.org/10.1155/2022/6015877","url":null,"abstract":"<p><strong>Background: </strong>Gastric cancer is one of the most common malignant tumors in the world. Albumin paclitaxel (Nab-PTX) is a novel microtubule inhibitor with albumin as the carrier. Several clinical trials are underway in gastric cancer, but the autophagy mechanism of Nab-PTX on gastric cancer is still unclear. The autophagy and apoptosis effects of Nab-PTX compared with 5-pentafluorouracil (5-Fu) and lobaplatin (LBP) in gastric cancer are also unclear.</p><p><strong>Objective: </strong>This article will compare the effects of Nab-PTX, 5-Fu, LBP, and albumin paclitaxel + 5-pentafluorouracil (Nab-PTX + 5-Fu) on AGS cells from the perspective of autophagy and apoptosis, which is to provide new ideas and experimental evidence for gastric cancer.</p><p><strong>Method: </strong>(1) Experimental groups were control (Ctrl), Nab-PTX, 5-Fu, LBP, and Nab-PTX + 5-Fu. (2) CCK-8 assay was used to reflect cell viability and proliferation. (3) The flow cytometry was used to perform the 24-hour apoptosis and cell cycle of each group. (4) Western blot assay was used to investigate autophagy signal proteins LC3I/LC3II, LC3II/LC3I, SQSTM1/p62, Beclin-1, Atg12, Atg5, p-mULK1, p-AMPK, p-mTOR, and apoptosis signal proteins Bax and Bcl-2.</p><p><strong>Results: </strong>Nab-PTX, 5-Fu, LBP, and Nab-PTX + 5-Fu inhibited AGS cells' proliferation and arrested the cell cycle. At the same time, each group increased the apoptosis of AGS cells to various degrees (Nab-PTX + 5-Fu > Nab-PTX > 5-Fu > LBP, respectively). The experimental results showed that Nab-PTX and Nab-PTX + 5-Fu promoted autophagy and apoptosis of AGS cells. The comparison of Nab-PTX, 5-Fu, and LBP between groups revealed that 5-Fu inhibited autophagy and the expression of apoptosis protein Bax. In LBP, abnormal activation of autophagy downstream, blocking of autophagy flow, abnormal increase of ATG12, and increased expression of apoptosis protein Bax occurred. Further study found that the autophagy upstream mechanism is different.</p><p><strong>Conclusion: </strong>Nab-PTX, 5-Fu, LBP, and Nab-PTX + 5-Fu can inhibit cell proliferation, promote cell apoptosis, and induce the difference in autophagy expression. The autophagy difference of this antitumor drug may be related to its inducing apoptosis. Meanwhile, Nab-PTX has a better antitumor effect than 5-Fu and LBP in gastric cancer, and the combination of Nab-PTX + 5-Fu has more antitumor advantages.</p>","PeriodicalId":48755,"journal":{"name":"Canadian Journal of Gastroenterology and Hepatology","volume":" ","pages":"6015877"},"PeriodicalIF":2.7,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9205742/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40012227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computed Tomography Images of Spontaneous Portosystemic Shunt in Liver Cirrhosis.","authors":"Fangfang Yi, Xiaozhong Guo, Qing-Lei Zeng, Benqiang Yang, Yanglan He, Shanshan Yuan, Ankur Arora, Xingshun Qi","doi":"10.1155/2022/3231144","DOIUrl":"10.1155/2022/3231144","url":null,"abstract":"<p><p>Spontaneous portosystemic shunt (SPSS) refers to collateral vessels that communicate between the portal vein system and systemic circulation. SPSS mainly includes esophageal varices, gastric varices, left gastric vein, recanalized paraumbilical vein, abdominal wall varices, and spontaneous splenorenal shunt. SPSS contributes to the development of hepatic encephalopathy caused by portal vein inflow bypassing and carries a higher risk of death in liver cirrhosis. Abdominal contrast-enhanced computed tomography is a major imaging approach to establish a diagnosis of SPSS and evaluate its location and feature. This review primarily describes the main contrast-enhanced CT features of SPSS in liver cirrhosis.</p>","PeriodicalId":48755,"journal":{"name":"Canadian Journal of Gastroenterology and Hepatology","volume":" ","pages":"3231144"},"PeriodicalIF":2.7,"publicationDate":"2022-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200601/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40012326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clostridioides difficile Infection in Liver Cirrhosis: A Concise Review","authors":"Yuanbin Liu, Mingkai Chen","doi":"10.1155/2022/4209442","DOIUrl":"https://doi.org/10.1155/2022/4209442","url":null,"abstract":"Clostridium difficile is a Gram-positive bacillus with fecal-oral transmission and is currently one of the most common nosocomial infections worldwide, which was renamed Clostridioides difficile in 2016. Clostridioides difficile infection (CDI) is a prevalent infection in cirrhosis and negatively affects prognosis. This study aimed to provide a concise review with clinical practice implications. The prevalence of CDI in cirrhotic patients increases, while the associated mortality decreases. Multiple groups of risk factors increase the likelihood of CDI in patients with cirrhosis, such as antibiotic use, the severity of cirrhosis, some comorbidities, and demographic aspects. Treatment in the general population is currently described in the latest guidelines. In patients with cirrhosis, rifaximin and lactulose have been shown to reduce CDI risk due to their modulatory effects on the intestinal flora, although conflicting results exist. Fecal microbiota transplantation (FMT) as a treatment for the second or subsequent CDI recurrences has demonstrated a good safety and efficacy in cirrhosis and CDI. Future validation in more prospective studies is needed. Screening of asymptomatic patients appears to be discouraged for the prevention currently, with strict hand hygiene and cleaning of the ward and medical equipment surfaces being the cornerstone of minimizing transmission.","PeriodicalId":48755,"journal":{"name":"Canadian Journal of Gastroenterology and Hepatology","volume":"91 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2022-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90712194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Challenges of <i>Helicobacter pylori</i> Infection in Ethiopia: A Community-Based Cross-Sectional Study.","authors":"Esayas Kebede Gudina,&nbsp;Hiwot Amare,&nbsp;Solomon Ali,&nbsp;Melkamu Berhane Arefayine,&nbsp;Dagmawi Tewolde,&nbsp;Million Tesfaye Eshete,&nbsp;Mulusew Gerbaba Jebena,&nbsp;Andreas Wieser,&nbsp;Guenter Froeschl,&nbsp;Markos Tesfaye,&nbsp;Hailemichael Desalegn,&nbsp;Mulatu Gashaw","doi":"10.1155/2022/4013020","DOIUrl":"https://doi.org/10.1155/2022/4013020","url":null,"abstract":"<p><strong>Background: </strong>In resource-constrained countries, accurate diagnosis of <i>Helicobacter pylori</i> infection remains a challenge. This study aimed to assess the clinical utility of locally available serological and stool antigen test kits in the management of people with suspected <i>H. pylori</i> infection in Ethiopia.</p><p><strong>Methods: </strong>A community-based cross-sectional study was conducted with apparently healthy adults and children living in southwest Ethiopia. Participants were interviewed for dyspepsia symptoms and related clinical conditions. <i>H. pylori</i> infection was examined using commercially available serological and stool antigen tests. The association between <i>H. pylori</i> tests and dyspepsia symptoms was analyzed using logistic regression models.</p><p><strong>Results: </strong>Out of 1392 participants included in the final analysis, 49.1% and 6.5% tested positive for <i>H. pylori</i> infection with serology and stool antigen test kits, respectively. Participants reporting epigastric symptoms in the past three months (AOR = 1.93, 95% CI = 1.28-2.91) and those with recent dyspepsia treatment (AOR = 1.51, 95% CI = 1.05-2.18) were likely to have positive serology test. However, no association between dyspepsia symptoms and <i>H. pylori</i> stool antigen positivity was observed in our study.</p><p><strong>Conclusion: </strong>ccurate detection of <i>H. pylori</i> infections using commercially accessible diagnostics remains difficult in Ethiopia. With these methods, it will be hard to ensure adequate diagnosis and early treatment of <i>H. pylori</i> infection, as well as rational antibiotic use.</p>","PeriodicalId":48755,"journal":{"name":"Canadian Journal of Gastroenterology and Hepatology","volume":" ","pages":"4013020"},"PeriodicalIF":2.7,"publicationDate":"2022-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9553705/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33515097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COL11A1 is Downregulated by miR-339-5p and Promotes Colon Carcinoma Progression","authors":"Weizhi Liu, Ke Meng","doi":"10.1155/2022/8116990","DOIUrl":"https://doi.org/10.1155/2022/8116990","url":null,"abstract":"The roles of COL11A1 in cancer have been increasingly considered, but the understandings of the effects of COL11A1 on colon carcinoma progress are much limited yet. qRT-PCR and Western blot were utilized to evaluate COL11A1 expression at mRNA and protein levels, respectively, in colon carcinoma cell lines. Afterward, the tumorigenesis biological effects of COL11A1 were examined by CCK-8, colony formation, Transwell, and wound healing methods. Moreover, upstream miRNAs containing the binding sites with COL11A1 were predicted by the bioinformatics methods. The interplay between COL11A1 and miR-339-5p was identified by a dual-luciferase assay. COL11A1 expression was prominently upregulated in colon carcinoma cell lines relative to that in normal human colon mucosal epithelial cell lines, and it was related to tumor stages. The outcomes of in-vitro experiments suggested that interfering with COL11A1 remarkably repressed the malignant behaviors of SW480 and SW620 cells. MiR-339-5p was markedly lowly expressed in colon carcinoma cell lines. Furthermore, miR-339-5p directly targeted and negatively regulated COL11A1 expression. COL11A1 upregulation promoted colon carcinoma cell functions, while overexpressing miR-339-5p evidently attenuated the promotion. These results proved the modulation of the miR-339-5p/COL11A1 axis in colon carcinoma cells, and miR-339-5p repressed colon carcinoma progression via COL11A1 downregulation. These results offer new underlying targets for the accurate therapy of colon carcinoma patients.","PeriodicalId":48755,"journal":{"name":"Canadian Journal of Gastroenterology and Hepatology","volume":"46 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2022-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75770629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LncRNA PVT1 Promotes Cell Proliferation, Invasion, and Migration and Inhibits Cell Apoptosis by Phosphorylating YAP","authors":"Kaiyue Ji, Qi Zhang, W. Song, Tao Mao, Jing Guo, Congcong Min, Cuiping Zhang, Z. Tian, Xiaoyu Li","doi":"10.1155/2022/5332129","DOIUrl":"https://doi.org/10.1155/2022/5332129","url":null,"abstract":"Gastric cancer (GC) as a serious global health problem is a threat to human longevity. Plasmacytoma variant translocation 1 (PVT1) participates in the formation and progression of various cancers, including GC. The aim of this study is to investigate the mechanism underlying the functions of PVT1 and explore a novel target for the diagnosis and treatment of GC. Analysis of the TCGA dataset using the R software identified that the lncRNA PVT1 was greatly upregulated in GC tissues. Twenty pairs of GC and adjacent normal tissues were acquired from patients with GC, and the expression of PVT1 was evaluated using RT-qPCR. Furthermore, PVT1 expression was knocked down in GC cells using siRNA, and the GC cells were divided into control, negative control (NC), and siRNA groups. Cell proliferation ability was analyzed using Cell Counting Kit-8 (CCK8) and colony formation assays, whereas cell migration and invasion ability were investigated through wound healing and Transwell assays. Moreover, Western blotting was used to analyze the expression of Yes-associated protein (YAP) and epithelial-to-mesenchymal transition (EMT) proteins. We also found that PVT1 and YAP expressions were upregulated in the GC tissues compared with those in the adjacent nontumor tissues. Knockdown of PVT1 was found to inhibit the proliferation, invasion, and migration and promote apoptosis of GC cells. Furthermore, knockdown of PVT1 downregulated YAP and promoted phosphorylation of YAP, suggesting that PVT1 exerts actions on GC cells by targeting YAP and inhibits cell apoptosis in vitro. The EMT process was also inhibited by the knockdown of PVT1. In summary, lncRNA PVT1 facilitated cell proliferation, invasion, and migration and suppressed cell apoptosis by targeting YAP. This study suggests that the expressions of PVT1 and YAP could be used for the early detection of GC and the occurrence and development of GC could be inhibited by interfering the interaction of PVT1 and YAP, which will provide new insights for the diagnosis, treatment, and prognosis of GC.","PeriodicalId":48755,"journal":{"name":"Canadian Journal of Gastroenterology and Hepatology","volume":"101 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2022-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84064009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors Associated with the Prevalence of Hepatitis B among Volunteer Blood Donors at Jimma Blood Bank, South Ethiopia","authors":"H. Yigezu, Juhar Temam, Mitiku Bajiro, Leta Tesfaye Jule, N. Nagaprasad, A. Roy, A. Saka, K. Ramaswamy","doi":"10.1155/2022/7458747","DOIUrl":"https://doi.org/10.1155/2022/7458747","url":null,"abstract":"Background Hepatitis B is a severe, widespread infectious disease of the liver that affects millions of people around the world. It is one of the life-threatening liver infections caused by the hepatitis B virus (HBV). HBV is the cause of up to 80% of cases of primary liver cancer. Due to the potential risk associated with HBV infection, it is important to study the factors which are associated with the seropositive volunteers. Objective The purpose of this study was to identify factors associated with seropositivity for the hepatitis B virus among volunteers who donated blood at the Jimma Blood Bank in southern Ethiopia. Methods Cross-sectional research was conducted on blood donors who came to the Jimma Blood Bank to donate their blood. Three hundred and fifty-nine volunteer blood donors who arrived at the Jimma Blood Bank were investigated face-to-face in order to collect sociodemographic characteristics and risk factors for HBV infection. The data were analyzed using statistical software SPSS version 20.0. The association between the risk factor for HBV infection and HBV infection was determined using chi-square tests. Result In total, there were 359 participants; their mean age was 22.5, among which 161 (44.8%) were males. Out of 359 volunteers, 13 (3.6%) were seropositive for HBsAg. The test positivity rate among males was 7/198 (3.54%), while the rate among females was 6/161 (3.7%). More than 3/4 of those who tested positive were under the age of 40. Chi-square analysis showed that volunteers whose income was between 12 and 26.84 USD were less likely to have the infectious disease than those whose income was less than 11.84 USD per month (p=0.042). Conclusion The prevalence of HBV was found to be 3.6% among selected volunteers. It was found that, out of 20 volunteers, 13 had infection. Chi-square analysis showed that HBV infection was associated with low monthly income and the use of unsafe therapeutic injections.","PeriodicalId":48755,"journal":{"name":"Canadian Journal of Gastroenterology and Hepatology","volume":"33 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2022-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76165365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}