Research Journal of Biotechnology最新文献

{"title":"Facile Preparation and Characterization of Alginate and Triton X-100 Zinc Phosphate modified Surface Nanocrystals as a Novel Anti-corrosive and Antibacterial agent","authors":"Arezoo Mohammadkhani, Sadjadi Mirabdullah Seyed, N. Farhadyar, F. Mohammadkhani","doi":"10.25303/1812rjbt910101","DOIUrl":"https://doi.org/10.25303/1812rjbt910101","url":null,"abstract":"In this study, two surface-modified antibacterial and anti-corrosive nanoparticles (ZnP-T, ZnP-ALG) were synthesized by facile and efficient one-step ultrasonic synthesis method. Nanocrystals were characterized by X-ray Diffraction (XRD), Fourier Transform Infrared (FT-IR), Energy Dispersive X-Ray Analysis (EDX), Scanning Electron Microscopy (SEM) and Transmission electron microscopy (TEM). The TEM image showed that the product had good dispersion with a particle size of 10-23, 15–32 nm respectively. Antibacterial and cytocompatibility properties were studied by agar diffusion, in vitro viability and cytotoxicity assay (MTT) and colony-forming-unit (CFU). Antibacterial rate of the ZnP-T and ZnP-ALG nanoparticles reached near 100% and cell viability was near 100%. The corrosion resistance of ZnP-T and ZnP-ALG was evaluated by the electrochemical impedance spectroscopy (EIS) approach. Nyquist diagrams demonstrated that the ZnP-ALG sample had better protection than the ZnP-T sample at all immersion time . The frequency phase angle of the ZnP-ALG sample (θ10 kHz) has a greater value compared with the ZnP-T case.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"11 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139288743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calotropis procera leaf extract against paracetamol induced hepatotoxicity in Rats by regulating JNK/ASK-1 Signaling Pathway","authors":"K. K. Agrawal, Y. Murti","doi":"10.25303/1812rjbt1220129","DOIUrl":"https://doi.org/10.25303/1812rjbt1220129","url":null,"abstract":"The goal of the study was to assess the hepatoprotective efficacy of young Calotropis procera leaves. The young leaves of Calotropis procera were extracted by cold maceration with ultra-sonication using chloroform, ethyl acetate and distilled water. Male Wistar rats were used for hepatoprotective evaluation and the body weight, liver weight, serum biomarkers and anti-oxidant markers were measured during study. The extract/fractions significantly (P<0.05) increased the absolute and relative weight of the liver of rats at the dose of 200 mg/kg. It also suppressed the serum biomarkers (P<0.05) in the aforementioned doses. Oral administration of extract/fractions significantly increase the oxidative marker and decreased the progression of paracetamol induced hepatotoxicity. The result of the study suggested that Calotropis procera leaf extract/fractions can reduce hepatotoxicity caused by paracetamol.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139289152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant and Antibacterial activity of Lotus tea in Thua Thien Hue province from Vietnam","authors":"N. T. Q. Trang, Ngo Quy Thao Ngoc, Dang Thanh Long, Pham Thanh","doi":"10.25303/1812rjbt048054","DOIUrl":"https://doi.org/10.25303/1812rjbt048054","url":null,"abstract":"In this study, we determined the antioxidant and antibacterial activity in different types of lotus tea (Nelumbo nucifera Gaernt.) in Thua Thien Hue province, Vietnam. The results showed that in four types of lotus tea, the catalase enzyme activity was 0.92-1.35 U/mg protein, the peroxidase enzyme activity was 0.97-2.67 U/mg protein, ascorbic acid content was 0.088-0.135%, the IC50 value was 4.98-6.03 mg/mL and all four kinds of lotus tea inhibited bacteria to a variable extent with low sensitivity. The inhibition zone’s mean diameter increased from 0.23 cm to 0.77 cm. The flower lotus tea gave the highest results on most of the research criteria and the lowest was lotus rhizome tea. From obtained results, tea produced from lotus plants grown in Thua Thien Hue province, Vietnam has a high potential for antioxidant and antibacterial properties.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"45 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139289182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The repurposing of FDA-approved drugs to stop viral replication in COVID-19 treatment: a comprehensive molecular docking and dynamics analysis","authors":"Dammalli Manjunath, S.G. Bhavya, Murthy K. R. Shadakshara, S. Rajashekhara, B.E. Rangaswamy","doi":"10.25303/1812rjbt055066","DOIUrl":"https://doi.org/10.25303/1812rjbt055066","url":null,"abstract":"Amidst a public health crisis such as the SARS-CoV-2 induced COVID-19 pandemic, the urgency to develop and provide access to novel drugs becomes paramount. In these scenarios, repurposing existing drugs emerges as an appealing strategy due to their established safety profiles and prior regulatory approvals, potentially streamlining their adoption for new therapeutic purposes. There has been a notable interest in investigating the repurposing potential of FDA-approved drugs to combat the disease triggered by the SARS-CoV-2 virus. This investigation was conducted with the objective of identifying FDA-approved antiviral drugs that could target both the original and mutant forms of the SARS-CoV-2 RNA-dependent RNA polymerase (RdRp) protein, given the escalating prevalence of SARS-CoV-2 mutations that undermined vaccine efficacy and underscored the need for alternative treatment avenues. Despite ongoing clinical trials, the selection of drugs specifically designed to combat SARS-CoV-2 remains limited. The present study aimed to assess the inhibitory potential of eight approved antiviral drugs. The wild-type and mutant (P323L) RdRp protein (PDB ID: 7BV2). By utilizing molecular docking techniques, the selected drugs were evaluated based on their binding affinities with both the mutant and wild-type RdRp protein. Subsequently, the protein-ligand complexes exhibiting the highest binding affinities were subjected to a 150 ns molecular dynamics simulation to evaluate their stability. The outcomes of the molecular docking analysis pinpointed Dolutegravir and Beclabuvir as possessing the most robust binding affinities for both mutant and wild-type RdRp systems. These findings prompted the selection of these drugs for an in-depth molecular dynamic simulation (MDS) investigation. The comprehensive analysis of Root Mean Square Deviation (RMSD), Root Mean Square Fluctuation (RMSF), Radius of Gyration (Rg), Solvent Accessible Surface Area (SASA) and Secondary Structure Dynamics confirmed that Dolutegravir and Beclabuvir are potent inhibitors of the NSP12 protein. Collectively, the computational assessments put forth two potential contenders: Dolutegravir and Beclabuvir as promising inhibitors against both the wild-type and mutant RdRp proteins. To further validate these drug candidates, rigorous in vitro and in vivo investigations are warranted which could eventually position them as valuable therapeutic agents in the fight against COVID-19.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"80 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139289228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico studies on characterization of glucosinolate derivatives for inhibition ofcell cycle regulation protein BCL-2","authors":"Bi Maryam, Hoskeri H. Joy","doi":"10.25303/1812rjbt1350141","DOIUrl":"https://doi.org/10.25303/1812rjbt1350141","url":null,"abstract":"Glucosinolates (GSL) are well-known secondary metabolites present in plants belonging to the Brassicaceae family (Cabbage, broccoli, cauliflower and brussels sprouts) and have been studied for their pharmacological properties. Glucosinolate is a sulfur-rich anionic hydrolyzed product which is good for the human diet. Through the enriched diet, the myrosinase enzyme hydrolyzes glucosinolates to form isothiocyanates against bacterial and fungal infections. Glucosinolates also show antimicrobial, anti-inflammatory, anticancer and cholinesterase inhibitor activity. The present study is to evaluate the glucosinolate substructures retrieved from the chemical database and to evaluate to understand that the BCL-2 inhibition showed the tumor growth and induced apoptosis. We retrieved 117 glucosinolate compounds and in silico druglike prediction screened the compounds and molecular docking against the BCL-2 protein structure. Based on the druglikeness analysis, we identified 39 compounds and these compounds were used for the inhibitory study with BCL2 protein. The results show that 2-Methyl-2-propenyl glucosinolate, 4-Methylpentyl glucosinolate, 4-Mercaptobutyl glucosinolate, Glucocapparin, Glucoerucin, Glucolepidiin, Glucoputranjivin, Glucoviorylin, n-Butyl glucosinolate compounds are strongly binding to BCL-2 protein structure by forming three hydrogen bonds and the resultant compounds can be used as a potential lead molecule for BCL-2 inhibitors.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"55 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139288753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on in vitro therapeutic impact of 17-ꞵ Estradiol on Glioblastoma cell lines","authors":"K. Ashwini, Vijith V. Shetty, A. Raghotham, Praveen Kumar Shetty, K. Suchetha","doi":"10.25303/1812rjbt0106","DOIUrl":"https://doi.org/10.25303/1812rjbt0106","url":null,"abstract":"Glioblastomas (GBM) or grade IV astrocytomas is the most aggressive and frequent tumor in the Central Nervous System (CNS). GBM treatment mainly consists of surgical resection and radio and/or chemotherapy. However, due to its infiltration capacity, it is practically impossible to completely extract the tumor and it relapses. Aberrant EGFR expression is observed in GBM including EGFR mutation, rearrangement and deletions. Blood brain barrier is one of the major obstacles which prevents the entry of larger molecules into the brain. Hence a small molecule, with lesser neurotoxicity should be developed in treating GBM or any other brain related diseases. Previous studies have reported the therapeutic potency of 17-ꞵ Estradiol in treating breast cancer, but little is known about the therapeutic role of estrogen signalling in the development and progression of gliomas. Estradiol being the small molecule has molecular weight of 272.4g/mol. It is a lipophilic molecule and therefore it can pass through the blood brain barrier by passive diffusion and could be considered as a therapeutic molecule in treating GBM. Present study showed that 17-ꞵ Estradiol inhibited the migration, colony formation in GBM cell lines. Further it induced early and late apoptosis and arrested the cell cycle at S-Phase of the cell cycle. EGFR and downstream EGFR associated protein expression were also reduced upon exposure of GBM cell lines to E2. These results show that 17-ꞵ Estradiol could be considered as a therapeutic molecule in GBM treatment.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139289172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An assessment of the application of magnetic fields in the study of in vitro plant cell and tissue cultivation","authors":"S. Narasimhan, S. Bindu","doi":"10.25303/1812rjbt1470150","DOIUrl":"https://doi.org/10.25303/1812rjbt1470150","url":null,"abstract":"The magnetic field can affect the structure, physiology and biochemistry of plants. The use of magnetic fields on cultured plant cells and tissues is a proven fact. The system to deliver magnetic flux is very important. Experiments have been performed by directly placing cultures in the vicinity of the magnetic field from a permanent magnetic source. Such a system delivered a fixed magnetic strength. Electric magnets are a better mode to deliver magnetic field to plants because it is easy to deliver tailor-made magnetic exposure. Majority of such studies utilized a magnetic field in the order of milliteslas, some of them utilized a high magnetic field upto 17.8 Tesla. The experiments also utilized varying exposure times and differential exposure to the north or south pole. Such studies reported either an increase or decrease of many parameters such as cell size, shape, metabolite content, chlorophyll content and enzyme activity. Most of the studies discussing magnetotropism on plants utilized seeds or intact plants to find out the effect of magnetic fields on plants. Such studies also give some understanding of the mechanism of action. Using a plant cell culture system, it is possible to grow single cells and small cell aggregates. Therefore, in vitro systems offer an excellent tool to understand the mechanism of action at the cellular level.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139289194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study of phytochemicals, antioxidant and anti-inflammatory activity of infusion and decoction extracts from Pycnoporus sanguineus (L.) Murril collected in West Bengal","authors":"Sudip Mondal, Rituparna Saha, Bijoy Mal, K. Acharya, Gunjan Biswas","doi":"10.25303/1812rjbt07013","DOIUrl":"https://doi.org/10.25303/1812rjbt07013","url":null,"abstract":"Mushrooms are well-known pharmaceutical agents as they possess bioactive secondary metabolites along with a large amount of proteins, carbohydrates, crude fiber, fat and other beneficial compounds. Due to their remarkable pharmacological property, they are used in traditional medicine in different countries of Asia. Pycnoporus sanguineus is a polypore fungus having a wide variety of bioactive substances and therapeutic activities that are reported previously in different countries of the world. The present study is focused to estimate different bioactive compounds and evaluate antioxidant and anti-inflammatory activities of P. sanguineus using two different extraction procedures (infusion and decoction). In this study, total phenol, total flavonoid, β-carotene, lycopene and ascorbic acid contents are estimated. Antioxidant and inti-inflammatory activities are investigated by total antioxidant capacity, DPPH radical scavenging, ABTS radical scavenging, chelating of ferrous ion (Fe2+), reducing power assays and protein denaturation assay respectively. The aim of the study is to establish P. sanguineus as a natural drug that could be used against oxidative stress and inflammation-related diseases substituting commercial drugs.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"76 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139288736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosensors from the Perspective of Sensing Labelled and Unlabelled Targets","authors":"S. Chhotaray, S. Jal","doi":"10.25303/1812rjbt1510167","DOIUrl":"https://doi.org/10.25303/1812rjbt1510167","url":null,"abstract":"Biosensors are target-specific affinity based analytical tools. Their applications are vast in areas such as clinical diagnosis, food quality, environment monitoring and in other fields as well where timely and reliable analysis are required. Biosensing technology is gradually developing to produce nove.l biosensors for point-of-care diagnosis. The involvement of new advancements such as aptamer-based technologies and nano-bioelectronic technologies have great potentialto lead to the development of next generation biosensors with improved sensitivity, specificity, portability and cost effectiveness. This review emphasizes several aspects of biosensors such as their classification, mechanism involved and their biomedical applications.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"173 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139289209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Copper levels, Cadmium toxicity and presence of Taste anomalies as predictors for early diagnosis of Type 2 Diabetes Mellitus","authors":"C. Shaji, Radha Saraswathy","doi":"10.25303/1812rjbt1140121","DOIUrl":"https://doi.org/10.25303/1812rjbt1140121","url":null,"abstract":"The global prevalence of Type 2 diabetes mellitus (T2DM) has been observed to escalate annually. However, studies also report a higher prevalence of undiagnosed diabetes. This results in disease progression accompanied with development of macro and micro complications. Taste disorders are significant clinical manifestations for diabetes which have been ignored in majority of the cases. Certain trace elements are identified to be associated with the development and progression of diabetes and are also established to activate or suppress the functioning of human taste receptors. This study aims at determining the serum trace element concentrations in diabetic (n =153) and non-diabetic (n=153) subjects who have been screened for taste perception anomalies. Linear correlation of trace metal levels with glucose levels showed a slightly positive association of Cu, Cr and Cd with FBG. Multiple logistic regression model predicted Cu levels, Cd toxicity and taste anomaly as significant biomarkers for T2DM. This would enable early diagnosis of T2DM, thus facilitating appropriate medical intervention to avoid multiple complications in T2DM individuals.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":"227 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139289305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}