Research Journal of Biotechnology最新文献_第5页

In silico studies on characterization of glucosinolate derivatives for inhibition ofcell cycle regulation protein BCL-2 抑制细胞周期调控蛋白 BCL-2 的葡萄糖苷酸衍生物特征的硅学研究

IF 0.2

Research Journal of Biotechnology Pub Date : 2023-11-05 DOI: 10.25303/1812rjbt1350141

Bi Maryam, Hoskeri H. Joy

{"title":"In silico studies on characterization of glucosinolate derivatives for inhibition ofcell cycle regulation protein BCL-2","authors":"Bi Maryam, Hoskeri H. Joy","doi":"10.25303/1812rjbt1350141","DOIUrl":"https://doi.org/10.25303/1812rjbt1350141","url":null,"abstract":"Glucosinolates (GSL) are well-known secondary metabolites present in plants belonging to the Brassicaceae family (Cabbage, broccoli, cauliflower and brussels sprouts) and have been studied for their pharmacological properties. Glucosinolate is a sulfur-rich anionic hydrolyzed product which is good for the human diet. Through the enriched diet, the myrosinase enzyme hydrolyzes glucosinolates to form isothiocyanates against bacterial and fungal infections. Glucosinolates also show antimicrobial, anti-inflammatory, anticancer and cholinesterase inhibitor activity. The present study is to evaluate the glucosinolate substructures retrieved from the chemical database and to evaluate to understand that the BCL-2 inhibition showed the tumor growth and induced apoptosis. We retrieved 117 glucosinolate compounds and in silico druglike prediction screened the compounds and molecular docking against the BCL-2 protein structure. Based on the druglikeness analysis, we identified 39 compounds and these compounds were used for the inhibitory study with BCL2 protein. The results show that 2-Methyl-2-propenyl glucosinolate, 4-Methylpentyl glucosinolate, 4-Mercaptobutyl glucosinolate, Glucocapparin, Glucoerucin, Glucolepidiin, Glucoputranjivin, Glucoviorylin, n-Butyl glucosinolate compounds are strongly binding to BCL-2 protein structure by forming three hydrogen bonds and the resultant compounds can be used as a potential lead molecule for BCL-2 inhibitors.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139288753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on in vitro therapeutic impact of 17-ꞵ Estradiol on Glioblastoma cell lines 研究 17-ꞵ 雌二醇对胶质母细胞瘤细胞系的体外治疗影响

IF 0.2

Research Journal of Biotechnology Pub Date : 2023-11-05 DOI: 10.25303/1812rjbt0106

K. Ashwini, Vijith V. Shetty, A. Raghotham, Praveen Kumar Shetty, K. Suchetha

{"title":"Study on in vitro therapeutic impact of 17-ꞵ Estradiol on Glioblastoma cell lines","authors":"K. Ashwini, Vijith V. Shetty, A. Raghotham, Praveen Kumar Shetty, K. Suchetha","doi":"10.25303/1812rjbt0106","DOIUrl":"https://doi.org/10.25303/1812rjbt0106","url":null,"abstract":"Glioblastomas (GBM) or grade IV astrocytomas is the most aggressive and frequent tumor in the Central Nervous System (CNS). GBM treatment mainly consists of surgical resection and radio and/or chemotherapy. However, due to its infiltration capacity, it is practically impossible to completely extract the tumor and it relapses. Aberrant EGFR expression is observed in GBM including EGFR mutation, rearrangement and deletions. Blood brain barrier is one of the major obstacles which prevents the entry of larger molecules into the brain. Hence a small molecule, with lesser neurotoxicity should be developed in treating GBM or any other brain related diseases. Previous studies have reported the therapeutic potency of 17-ꞵ Estradiol in treating breast cancer, but little is known about the therapeutic role of estrogen signalling in the development and progression of gliomas. Estradiol being the small molecule has molecular weight of 272.4g/mol. It is a lipophilic molecule and therefore it can pass through the blood brain barrier by passive diffusion and could be considered as a therapeutic molecule in treating GBM. Present study showed that 17-ꞵ Estradiol inhibited the migration, colony formation in GBM cell lines. Further it induced early and late apoptosis and arrested the cell cycle at S-Phase of the cell cycle. EGFR and downstream EGFR associated protein expression were also reduced upon exposure of GBM cell lines to E2. These results show that 17-ꞵ Estradiol could be considered as a therapeutic molecule in GBM treatment.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139289172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An assessment of the application of magnetic fields in the study of in vitro plant cell and tissue cultivation 磁场在离体植物细胞和组织培养研究中的应用评估

IF 0.2

Research Journal of Biotechnology Pub Date : 2023-11-05 DOI: 10.25303/1812rjbt1470150

S. Narasimhan, S. Bindu

{"title":"An assessment of the application of magnetic fields in the study of in vitro plant cell and tissue cultivation","authors":"S. Narasimhan, S. Bindu","doi":"10.25303/1812rjbt1470150","DOIUrl":"https://doi.org/10.25303/1812rjbt1470150","url":null,"abstract":"The magnetic field can affect the structure, physiology and biochemistry of plants. The use of magnetic fields on cultured plant cells and tissues is a proven fact. The system to deliver magnetic flux is very important. Experiments have been performed by directly placing cultures in the vicinity of the magnetic field from a permanent magnetic source. Such a system delivered a fixed magnetic strength. Electric magnets are a better mode to deliver magnetic field to plants because it is easy to deliver tailor-made magnetic exposure. Majority of such studies utilized a magnetic field in the order of milliteslas, some of them utilized a high magnetic field upto 17.8 Tesla. The experiments also utilized varying exposure times and differential exposure to the north or south pole. Such studies reported either an increase or decrease of many parameters such as cell size, shape, metabolite content, chlorophyll content and enzyme activity. Most of the studies discussing magnetotropism on plants utilized seeds or intact plants to find out the effect of magnetic fields on plants. Such studies also give some understanding of the mechanism of action. Using a plant cell culture system, it is possible to grow single cells and small cell aggregates. Therefore, in vitro systems offer an excellent tool to understand the mechanism of action at the cellular level.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139289194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative study of phytochemicals, antioxidant and anti-inflammatory activity of infusion and decoction extracts from Pycnoporus sanguineus (L.) Murril collected in West Bengal 从西孟加拉邦采集的 Pycnoporus sanguineus (L.) Murril 的输液和煎煮提取物中提取的植物化学物质、抗氧化剂和抗炎活性的比较研究

IF 0.2

Research Journal of Biotechnology Pub Date : 2023-11-05 DOI: 10.25303/1812rjbt07013

Sudip Mondal, Rituparna Saha, Bijoy Mal, K. Acharya, Gunjan Biswas

{"title":"Comparative study of phytochemicals, antioxidant and anti-inflammatory activity of infusion and decoction extracts from Pycnoporus sanguineus (L.) Murril collected in West Bengal","authors":"Sudip Mondal, Rituparna Saha, Bijoy Mal, K. Acharya, Gunjan Biswas","doi":"10.25303/1812rjbt07013","DOIUrl":"https://doi.org/10.25303/1812rjbt07013","url":null,"abstract":"Mushrooms are well-known pharmaceutical agents as they possess bioactive secondary metabolites along with a large amount of proteins, carbohydrates, crude fiber, fat and other beneficial compounds. Due to their remarkable pharmacological property, they are used in traditional medicine in different countries of Asia. Pycnoporus sanguineus is a polypore fungus having a wide variety of bioactive substances and therapeutic activities that are reported previously in different countries of the world. The present study is focused to estimate different bioactive compounds and evaluate antioxidant and anti-inflammatory activities of P. sanguineus using two different extraction procedures (infusion and decoction). In this study, total phenol, total flavonoid, β-carotene, lycopene and ascorbic acid contents are estimated. Antioxidant and inti-inflammatory activities are investigated by total antioxidant capacity, DPPH radical scavenging, ABTS radical scavenging, chelating of ferrous ion (Fe2+), reducing power assays and protein denaturation assay respectively. The aim of the study is to establish P. sanguineus as a natural drug that could be used against oxidative stress and inflammation-related diseases substituting commercial drugs.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139288736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosensors from the Perspective of Sensing Labelled and Unlabelled Targets 从感知标记和未标记目标的角度看生物传感器

IF 0.2

Research Journal of Biotechnology Pub Date : 2023-11-05 DOI: 10.25303/1812rjbt1510167

S. Chhotaray, S. Jal

引用次数: 0

Copper levels, Cadmium toxicity and presence of Taste anomalies as predictors for early diagnosis of Type 2 Diabetes Mellitus 铜含量、镉毒性和味觉异常是早期诊断 2 型糖尿病的预测指标

IF 0.2

Research Journal of Biotechnology Pub Date : 2023-11-05 DOI: 10.25303/1812rjbt1140121

C. Shaji, Radha Saraswathy

{"title":"Copper levels, Cadmium toxicity and presence of Taste anomalies as predictors for early diagnosis of Type 2 Diabetes Mellitus","authors":"C. Shaji, Radha Saraswathy","doi":"10.25303/1812rjbt1140121","DOIUrl":"https://doi.org/10.25303/1812rjbt1140121","url":null,"abstract":"The global prevalence of Type 2 diabetes mellitus (T2DM) has been observed to escalate annually. However, studies also report a higher prevalence of undiagnosed diabetes. This results in disease progression accompanied with development of macro and micro complications. Taste disorders are significant clinical manifestations for diabetes which have been ignored in majority of the cases. Certain trace elements are identified to be associated with the development and progression of diabetes and are also established to activate or suppress the functioning of human taste receptors. This study aims at determining the serum trace element concentrations in diabetic (n =153) and non-diabetic (n=153) subjects who have been screened for taste perception anomalies. Linear correlation of trace metal levels with glucose levels showed a slightly positive association of Cu, Cr and Cd with FBG. Multiple logistic regression model predicted Cu levels, Cd toxicity and taste anomaly as significant biomarkers for T2DM. This would enable early diagnosis of T2DM, thus facilitating appropriate medical intervention to avoid multiple complications in T2DM individuals.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139289305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunomodulatory potential of aqueous extract of Solanum xanthocarpum with reference to splenocyte proliferation and cytokines induction in Wistar albino rats 黄花茄水提取物对 Wistar 白化大鼠脾细胞增殖和细胞因子诱导的免疫调节潜力

IF 0.2

Research Journal of Biotechnology Pub Date : 2023-11-05 DOI: 10.25303/1812rjbt1300134

Pradeep Choudhary, Vishal Khandelwal

{"title":"Immunomodulatory potential of aqueous extract of Solanum xanthocarpum with reference to splenocyte proliferation and cytokines induction in Wistar albino rats","authors":"Pradeep Choudhary, Vishal Khandelwal","doi":"10.25303/1812rjbt1300134","DOIUrl":"https://doi.org/10.25303/1812rjbt1300134","url":null,"abstract":"Limited study has been done so far to investigate immunomodulatory efficacy of whole plant of hot aqueous extract of Solanum xanthocarpum (HAESX) in Wistar albino rats. Present work has been designed to study immunomodulatory effect of HAESX in Wistar albino rat with respect to splenocyte proliferation and cytokines induction. Concanavalin A (Con A) activated splenocytes were used to measure in vitro cell mediated immune response using 3-(4, 5-Dimethylthiazolyl)-2, 5-diphenyl tetrazolium bromide (MTT) in the presence of varied doses of HAESX (20-500 μg/mL). The effects of HAESX on cell mediated immune response were studied using six groups of Wistar albino rats. Tumor necrosis factor-α (TNF-α), Interferon-γ (IFN-γ), Interleukin-10 (IL-10) and Interleukin-4 (IL-4) were measured in splenocyte culture supernatant by sandwich ELISA with different concentrations of HAESX (20-500 μg/mL). Study depicts significant decrease (p<0.05) in the proliferation of Con A treated splenocyte at 250 and 500 μg/mL HAESX, reflecting cell mediated immunoinhibitory potential of HAESX. Dose dependent significant increase (p<0.01) in the amount of IL-10 was found in splenocyte culture supernatant with increase in HAESX concentrations. TNF-α, IFN-γ and IL-4 were found to decrease significantly (p<0.01) with increase in HAESX concentrations. Study revealed that HAESX of Solanum xanthocarpum is found to influence the networking of cytokines, implying that it may play a function in regulating the activity of the host's immune system and can serve as potent herbal drug against immune rejection.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139288953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quality control of marketed products of Andrographis paniculata by HPLC analysis 通过高效液相色谱分析对穿心莲市场产品进行质量控制

IF 0.2

Research Journal of Biotechnology Pub Date : 2023-11-05 DOI: 10.25303/1812rjbt067070

B. Champati, A. Ray, S. Jena, A. Sahoo, S. Nayak

{"title":"Quality control of marketed products of Andrographis paniculata by HPLC analysis","authors":"B. Champati, A. Ray, S. Jena, A. Sahoo, S. Nayak","doi":"10.25303/1812rjbt067070","DOIUrl":"https://doi.org/10.25303/1812rjbt067070","url":null,"abstract":"Andrographis paniculata is one of the most important medicinal plants having several pharmaceutical properties. Various pharmaceutical products and raw powders of this herb are sold in the market. The high demand for A. paniculata poses the problem of adulteration in their products. Therefore, it becomes necessary to check the quality in terms of their major bioactive compounds. In the present study, several marketed products were analyzed for their quality assessment i.e. for the presence of desired bioactive constituent, andrographolide. Ten tested products of A. paniculata showed the presence of andrographolide. A validated High Performance Thin Layer Chromatography (HPLC) method has been used for the quantitative estimation of andrographolide. andrographolide was detected at a retention time (Rt) of 17.8±0.05 and was quantified using a linear regression equation. The amount of andrographolide in the marketed products was in the range of 0.01 to 7.08 %. The chromatographic fingerprints were developed in all marketed products in the study. Thus, the present study dealing with the detection and quantification of andrographolide through HPLC analysis would have enough significance in the quality control of marketed products of andrographis paniculata.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139289051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of potential inhibitors from Andrographis paniculata bioactive compounds against extended-spectrum β-lactamases through in silico and in vitro approaches 通过硅学和体外方法鉴定穿心莲生物活性化合物对广谱β-内酰胺酶的潜在抑制剂

IF 0.2

Research Journal of Biotechnology Pub Date : 2023-11-05 DOI: 10.25303/1812rjbt080090

Anupama Moirangthem, T. Baruah, Anand Prakash Maurya, A. Patar, Susmita Paul, Birson Ingti

{"title":"Identification of potential inhibitors from Andrographis paniculata bioactive compounds against extended-spectrum β-lactamases through in silico and in vitro approaches","authors":"Anupama Moirangthem, T. Baruah, Anand Prakash Maurya, A. Patar, Susmita Paul, Birson Ingti","doi":"10.25303/1812rjbt080090","DOIUrl":"https://doi.org/10.25303/1812rjbt080090","url":null,"abstract":"The present study aims to identify potential medicinal plant-based extended-spectrum β-lactamase (ESBL) inhibitors from Andrographis paniculata using both in silico and in vitro approaches. The ESBLs were obtained from the protein data bank. The structures of phytoconstituents were obtained from the PubChem database. The compounds were docked against different ESBLs (targeted proteins) using AutodockTools followed by molecular dynamics simulation. In silico results were further validated using in vitro testing through the disc diffusion method. The molecular docking revealed that most of the phytoconstituents have a good binding affinity. The binding energy and in vitro study of the phytoconstituents of Andrographis paniculata were compared with the standard inhibitor for ESBL i.e. clavulanic acid .14- Acetylandrographolide (AAD) showed good binding with the ESBL proteins, having the best values reported in the docking with OXA-10. Simulation of the complex of AAD and OXA-10 showed that the complex was relatively steady as evidenced by the lack of sudden fluctuations in the values of root mean square deviations, the radius of gyration and solvent-accessible surface area. Further confirmation of the in silico approach was done by an in vitro study against ESBL-producing organisms which showed inhibitory results. From this study, we can conclude that A.paniculata may have the potential to inhibit ESBLs and may be considered for treating bacterial infections.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139289378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flower waste as a potential substrate for biosurfactant production 将花卉废弃物作为生产生物表面活性剂的潜在基质

IF 0.2

Research Journal of Biotechnology Pub Date : 2023-11-05 DOI: 10.25303/1812rjbt071079

Julious Seikh, Jonali Owary

引用次数: 0