Bio-Design and Manufacturing最新文献

{"title":"<i>Fusobacterium nucleatum</i> FabG 3-ketoacyl-acyl carrier protein reductase: purification, crystallization, and X-ray crystallographic analysis","authors":"Shanru He, Yongbin Xu","doi":"10.34184/kssb.2023.11.3.50","DOIUrl":"https://doi.org/10.34184/kssb.2023.11.3.50","url":null,"abstract":"","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136276926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Candida auris</i> Aldo-keto reductase AldO: purification, crystallization, and X-ray crystallographic analysis","authors":"Jie Zhang, Xue Bai, Shanru He, Yongbin Xu","doi":"10.34184/kssb.2023.11.3.45","DOIUrl":"https://doi.org/10.34184/kssb.2023.11.3.45","url":null,"abstract":"","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136276928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crystal structure of the putative HOCl and HOSCN-responsive peroxiredoxin RclX from <i>Pseudomonas aeruginosa</i>","authors":"Nayeon Ki, Jiheon Kim, Doyeon Kim, Inseong Jo","doi":"10.34184/kssb.2023.11.3.39","DOIUrl":"https://doi.org/10.34184/kssb.2023.11.3.39","url":null,"abstract":"","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136276925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryo-electron tomography: a triumphant breakthrough in structural biology","authors":"Han-ul Kim, Mi Young An, Hyun Suk Jung","doi":"10.34184/kssb.2023.11.3.33","DOIUrl":"https://doi.org/10.34184/kssb.2023.11.3.33","url":null,"abstract":"","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136276924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accelerated fracture healing by osteogenic Ti45Nb implants through the PI3K–Akt signaling pathway","authors":"Jia Tan, Jiaxin Li, Zhaoyang Ran, Junxiang Wu, Dinghao Luo, Bojun Cao, Liang Deng, Xiaoping Li, Wenbo Jiang, Kai Xie, Lei Wang, Yongqiang Hao","doi":"10.1007/s42242-023-00250-6","DOIUrl":"https://doi.org/10.1007/s42242-023-00250-6","url":null,"abstract":"Abstract The key to managing fracture is to achieve stable internal fixation, and currently, biologically and mechanically appropriate internal fixation devices are urgently needed. With excellent biocompatibility and corrosion resistance, titanium–niobium alloys have the potential to become a new generation of internal fixation materials for fractures. However, the role and mechanism of titanium–niobium alloys on promoting fracture healing are still undefined. Therefore, in this study, we systematically evaluated the bone-enabling properties of Ti45Nb via in vivo and in vitro experiments. In vitro, we found that Ti45Nb has an excellent ability to promote MC3T3-E1 cell adhesion and proliferation without obvious cytotoxicity. Alkaline phosphatase (ALP) activity and alizarin red staining and semiquantitative analysis showed that Ti45Nb enhanced the osteogenic differentiation of MC3T3-E1 cells compared to the Ti6Al4V control. In the polymerase chain reaction experiment, the expression of osteogenic genes in the Ti45Nb group, such as ALP, osteopontin (OPN), osteocalcin (OCN), type 1 collagen (Col-1) and runt-related transcription factor-2 (Runx2), was significantly higher than that in the control group. Meanwhile, in the western blot experiment, the expression of osteogenic-related proteins in the Ti45Nb group was significantly increased, and the expression of PI3K–Akt-related proteins was also higher, which indicated that Ti45Nb might promote fracture healing by activating the PI3K–Akt signaling pathway. In vivo, we found that Ti45Nb implants accelerated fracture healing compared to Ti6Al4V, and the biosafety of Ti45Nb was confirmed by histological evaluation. Furthermore, immunohistochemical staining confirmed that Ti45Nb may promote osteogenesis by upregulating the PI3K/Akt signaling pathway. Our study demonstrated that Ti45Nb exerts an excellent ability to promote fracture healing as well as enhance osteoblast differentiation by activating the PI3K/Akt signaling pathway, and its good biosafety has been confirmed, which indicates its clinical translation potential. Graphic abstract","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"144 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134912547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microarrow sensor array with enhanced skin adhesion for transdermal continuous monitoring of glucose and reactive oxygen species","authors":"Xinshuo Huang, Baoming Liang, Shantao Zheng, Feifei Wu, Mengyi He, Shuang Huang, Jingbo Yang, Qiangqiang Ouyang, Fanmao Liu, Jing Liu, Hui-jiuan Chen, Xi Xie","doi":"10.1007/s42242-023-00246-2","DOIUrl":"https://doi.org/10.1007/s42242-023-00246-2","url":null,"abstract":"","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":" ","pages":""},"PeriodicalIF":7.9,"publicationDate":"2023-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44020951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyvinylpyrrolidone-based bioink: influence of bioink properties on printing performance and cell proliferation during inkjet-based bioprinting","authors":"Wei Long Ng, Xi Huang, V. Shkolnikov, R. Suntornnond, W. Yeong","doi":"10.1007/s42242-023-00245-3","DOIUrl":"https://doi.org/10.1007/s42242-023-00245-3","url":null,"abstract":"","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":" ","pages":""},"PeriodicalIF":7.9,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48543990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The prospects for bioprinting tumor models: recent advances in their applications","authors":"Changru Zhang, Xiaoqin Qiu, Yu Dai, Weiqing Kong, Yihao Liu, Haoyi Niu, Chengwei Wang, Xuelian Mi, Hui Wang, Ya Ren, Han Yang, K. Dai, Jinwu Wang","doi":"10.1007/s42242-023-00247-1","DOIUrl":"https://doi.org/10.1007/s42242-023-00247-1","url":null,"abstract":"","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"1 1","pages":""},"PeriodicalIF":7.9,"publicationDate":"2023-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41715538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proof of concept of a self-tightening needle-less suture using a NiTi shape memory alloy","authors":"A. Nespoli, Davide Ninarello, E. Bassani, V. Dallolio","doi":"10.1007/s42242-023-00254-2","DOIUrl":"https://doi.org/10.1007/s42242-023-00254-2","url":null,"abstract":"","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"6 1","pages":"536 - 549"},"PeriodicalIF":7.9,"publicationDate":"2023-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48219700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmaceutical digital transformation in China: digital quality assurance takes the driver’s seat","authors":"Yuanyuan Ge, Meng Cao, Hui Cao, Bin Han, Jingchen Zhang, Zhengyu Yi, Yaowei Wu, Beifen Zhu, Keping Ruan, Yiyi Pu, Yi Zeng, Tongjing Tao, Zhenzhong Lyu, Hongmei Yuan, Guiliang Chen","doi":"10.1007/s42242-023-00248-0","DOIUrl":"https://doi.org/10.1007/s42242-023-00248-0","url":null,"abstract":"","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"6 1","pages":"609 - 615"},"PeriodicalIF":7.9,"publicationDate":"2023-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42263432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}