Bio-Design and Manufacturing最新文献_第5页

A mixed-coordination electron trapping-enabled high-precision touch-sensitive screen for wearable devices 用于可穿戴设备的混合配位电子陷阱式高精度触摸屏

IF 7.9 1区医学

Bio-Design and Manufacturing Pub Date : 2024-07-10 DOI: 10.1007/s42242-024-00293-3

Xi Zhang, Junchi Ma, Hualin Deng, Jinming Zhong, Kaichen Xu, Qiang Wu, Bo Wen, Dongfeng Diao

{"title":"A mixed-coordination electron trapping-enabled high-precision touch-sensitive screen for wearable devices","authors":"Xi Zhang, Junchi Ma, Hualin Deng, Jinming Zhong, Kaichen Xu, Qiang Wu, Bo Wen, Dongfeng Diao","doi":"10.1007/s42242-024-00293-3","DOIUrl":"https://doi.org/10.1007/s42242-024-00293-3","url":null,"abstract":"<p>Touch-sensitive screens are crucial components of wearable devices. Materials such as reduced graphene oxide (rGO), carbon nanotubes (CNTs), and graphene offer promising solutions for flexible touch-sensitive screens. However, when stacked with flexible substrates to form multilayered capacitive touching sensors, these materials often suffer from substrate delamination in response to deformation; this is due to the materials having different Young’s modulus values. Delamination results in failure to offer accurate touch screen recognition. In this work, we demonstrate an induced charge-based mutual capacitive touching sensor capable of high-precision touch sensing. This is enabled by electron trapping and polarization effects related to mixed-coordinated bonding between copper nanoparticles and vertically grown graphene nanosheets. Here, we used an electron cyclotron resonance system to directly fabricate graphene–metal nanofilms (GMNFs) using carbon and copper, which are firmly adhered to flexible substrates. After being subjected to 3000 bending actions, we observed almost no change in touch sensitivity. The screen interaction system, which has a signal-to-noise ratio of 41.16 dB and resolution of 650 dpi, was tested using a handwritten Chinese character recognition trial and achieved an accuracy of 94.82%. Taken together, these results show the promise of touch-sensitive screens that use directly fabricated GMNFs for wearable devices.</p><h3 data-test=\"abstract-sub-heading\">Graphic abstract</h3>","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"18 1","pages":""},"PeriodicalIF":7.9,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141588052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nacre-inspired MXene-based film for highly sensitive piezoresistive sensing over a broad sensing range 在宽传感范围内实现高灵敏度压阻传感的珍珠层启发型 MXene 基薄膜

IF 7.9 1区医学

Bio-Design and Manufacturing Pub Date : 2024-07-02 DOI: 10.1007/s42242-024-00292-4

Gaofeng Wang, Lingxian Meng, Xinyi Ji, Xuying Liu, Jiajie Liang, Shuiren Liu

{"title":"Nacre-inspired MXene-based film for highly sensitive piezoresistive sensing over a broad sensing range","authors":"Gaofeng Wang, Lingxian Meng, Xinyi Ji, Xuying Liu, Jiajie Liang, Shuiren Liu","doi":"10.1007/s42242-024-00292-4","DOIUrl":"https://doi.org/10.1007/s42242-024-00292-4","url":null,"abstract":"<p>As the main component of wearable electronic equipment, flexible pressure sensors have attracted wide attention due to their excellent sensitivity and their promise with respect to applications in health monitoring, electronic skin, and human–computer interactions. However, it remains a significant challenge to achieve epidermal sensing over a wide sensing range, with short response/recovery time and featuring seamless conformability to the skin simultaneously. This is critical since the capture of minute electrophysiological signals is important for health care applications. In this paper, we report the preparation of a nacre-like MXene/sodium carboxymethyl cellulose (CMC) nanocomposite film with a “brick-and-mortar” interior structure using a vacuum-induced self-assembly strategy. The synergistic behavior of the MXene “brick” and flexible CMC “mortar” contributes to attenuating interlamellar self-stacking and creates numerous variable conductive pathways on the sensing film. This resulted in a high sensitivity over a broad pressure range (i.e., 0.03–22.37 kPa: 162.13 kPa<sup>−1</sup>; 22.37–135.71 kPa: 127.88 kPa<sup>−1</sup>; 135.71–286.49 kPa: 100.58 kPa<sup>−1</sup>). This sensor also has a low detection limit (0.85 Pa), short response/recovery time (8.58 ms/34.34 ms), and good stability (2000 cycles). Furthermore, we deployed pressure sensors to distinguish among tiny particles, various physiological signals of the human body, space arrays, robot motion monitoring, and other related applications to demonstrate their feasibility for a variety of health and motion monitoring use cases.</p><h3 data-test=\"abstract-sub-heading\">Graphic abstract</h3>\u0000","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"240 1","pages":""},"PeriodicalIF":7.9,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141522439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Implantable probe with integrated reference electrode for in situ neural signal and calcium ion monitoring 带集成参比电极的植入式探针，用于现场神经信号和钙离子监测

IF 7.9 1区医学

Bio-Design and Manufacturing Pub Date : 2024-07-01 DOI: 10.1007/s42242-024-00283-5

Junyu Xiao, Mengfei Xu, Longchun Wang, Bin Yang, Jingquan Liu

引用次数: 0

Flexible, high-density, laminated ECoG electrode array for high spatiotemporal resolution foci diagnostic localization of refractory epilepsy 用于难治性癫痫高时空分辨率病灶诊断定位的灵活、高密度、层叠式心电图电极阵列

IF 7.9 1区医学

Bio-Design and Manufacturing Pub Date : 2024-06-17 DOI: 10.1007/s42242-024-00278-2

Yafeng Liu, Zhouheng Wang, Yang Jiao, Ying Chen, Guangyuan Xu, Yinji Ma, Xue Feng

{"title":"Flexible, high-density, laminated ECoG electrode array for high spatiotemporal resolution foci diagnostic localization of refractory epilepsy","authors":"Yafeng Liu, Zhouheng Wang, Yang Jiao, Ying Chen, Guangyuan Xu, Yinji Ma, Xue Feng","doi":"10.1007/s42242-024-00278-2","DOIUrl":"https://doi.org/10.1007/s42242-024-00278-2","url":null,"abstract":"<p>High spatiotemporal resolution brain electrical signals are critical for basic neuroscience research and high-precision focus diagnostic localization, as the spatial scale of some pathologic signals is at the submillimeter or micrometer level. This entails connecting hundreds or thousands of electrode wires on a limited surface. This study reported a class of flexible, ultrathin, high-density electrocorticogram (ECoG) electrode arrays. The challenge of a large number of wiring arrangements was overcome by a laminated structure design and processing technology improvement. The flexible, ultrathin, high-density ECoG electrode array was conformably attached to the cortex for reliable, high spatial resolution electrophysiologic recordings. The minimum spacing between electrodes was 15 μm, comparable to the diameter of a single neuron. Eight hundred electrodes were prepared with an electrode density of 4444 mm<sup>−2</sup>. In focal epilepsy surgery, the flexible, high-density, laminated ECoG electrode array with 36 electrodes was applied to collect epileptic spike waves in rabbits, improving the positioning accuracy of epilepsy lesions from the centimeter to the submillimeter level. The flexible, high-density, laminated ECoG electrode array has potential clinical applications in intractable epilepsy and other neurologic diseases requiring high-precision electroencephalogram acquisition.</p>","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"38 1","pages":""},"PeriodicalIF":7.9,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141502128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly sensitive ratiometric fluorescent fiber matrices for oxygen sensing with micrometer spatial resolution 用于氧传感的高灵敏度比率荧光纤维矩阵，具有微米级空间分辨率

IF 7.9 1区医学

Bio-Design and Manufacturing Pub Date : 2024-04-29 DOI: 10.1007/s42242-024-00277-3

Giuliana Grasso, Valentina Onesto, Stefania Forciniti, Eliana D’Amone, Francesco Colella, Lara Pierantoni, Valeria Famà, Giuseppe Gigli, Rui L. Reis, Joaquim M. Oliveira, Loretta L. del Mercato

{"title":"Highly sensitive ratiometric fluorescent fiber matrices for oxygen sensing with micrometer spatial resolution","authors":"Giuliana Grasso, Valentina Onesto, Stefania Forciniti, Eliana D’Amone, Francesco Colella, Lara Pierantoni, Valeria Famà, Giuseppe Gigli, Rui L. Reis, Joaquim M. Oliveira, Loretta L. del Mercato","doi":"10.1007/s42242-024-00277-3","DOIUrl":"https://doi.org/10.1007/s42242-024-00277-3","url":null,"abstract":"<p>Oxygen (O<sub>2</sub>)-sensing matrices are promising tools for the live monitoring of extracellular O<sub>2</sub> consumption levels in long-term cell cultures. In this study, ratiometric O<sub>2</sub>-sensing membranes were prepared by electrospinning, an easy, low-cost, scalable, and robust method for fabricating nanofibers. Poly(ε-caprolactone) and poly(dimethyl)siloxane polymers were blended with tris(4,7-diphenyl-1,10-phenanthroline) ruthenium(II) dichloride, which was used as the O<sub>2</sub>-sensing probe, and rhodamine B isothiocyanate, which was used as the reference dye. The functionalized scaffolds were morphologically characterized by scanning electron microscopy, and their physicochemical profiles were obtained by Fourier transform infrared spectroscopy, thermogravimetric analysis, and water contact angle measurement. The sensing capabilities were investigated by confocal laser scanning microscopy, performing photobleaching, reversibility, and calibration curve studies toward different dissolved O<sub>2</sub> (DO) concentrations. Electrospun sensing nanofibers showed a high response to changes in DO concentrations in the physiological-pathological range from 0.5 to 20% and good stability under ratiometric imaging. In addition, the sensing systems were highly biocompatible for cell growth promoting adhesiveness and growth of three cancer cell lines, namely metastatic melanoma cell line SK-MEL2, breast cancer cell line MCF-7, and pancreatic ductal adenocarcinoma cell line Panc-1, thus recreating a suitable biological environment in vitro<i>.</i> These O<sub>2</sub>-sensing biomaterials can potentially measure alterations in cell metabolism caused by changes in ambient O<sub>2</sub> content during drug testing/validation and tissue regeneration processes.</p><h3 data-test=\"abstract-sub-heading\">Graphic abstract</h3>\u0000","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"73 1","pages":""},"PeriodicalIF":7.9,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140809114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovation leading development: a glimpse into three-dimensional bioprinting in Israel 创新引领发展：以色列三维生物打印技术一瞥

IF 7.9 1区医学

Bio-Design and Manufacturing Pub Date : 2024-04-29 DOI: 10.1007/s42242-024-00275-5

Lujing Gao, Zixuan Liu, Daniel Dikovsky, Jiqian Wang, Deqing Mei, Lihi Adler-Abramovich, Ehud Gazit, Kai Tao

{"title":"Innovation leading development: a glimpse into three-dimensional bioprinting in Israel","authors":"Lujing Gao, Zixuan Liu, Daniel Dikovsky, Jiqian Wang, Deqing Mei, Lihi Adler-Abramovich, Ehud Gazit, Kai Tao","doi":"10.1007/s42242-024-00275-5","DOIUrl":"https://doi.org/10.1007/s42242-024-00275-5","url":null,"abstract":"<p>Three-dimensional (3D) printing has attracted increasing research interest as an emerging manufacturing technology for developing sophisticated and exquisite architecture through hierarchical printing. It has also been employed in various advanced industrial areas. The development of intelligent biomedical engineering has raised the requirements for 3D printing, such as flexible manufacturing processes and technologies, biocompatible constituents, and alternative bioproducts. However, state-of-the-art 3D printing mainly involves inorganics or polymers and generally focuses on traditional industrial fields, thus severely limiting applications demanding biocompatibility and biodegradability. In this regard, peptide architectonics, which are self-assembled by programmed amino acid sequences that can be flexibly functionalized, have shown promising potential as bioinspired inks for 3D printing. Therefore, the combination of 3D printing and peptide self-assembly potentially opens up an alternative avenue of 3D bioprinting for diverse advanced applications. Israel, a small but innovative nation, has significantly contributed to 3D bioprinting in terms of scientific studies, marketization, and peptide architectonics, including modulations and applications, and ranks as a leading area in the 3D bioprinting field. This review summarizes the recent progress in 3D bioprinting in Israel, focusing on scientific studies on printable components, soft devices, and tissue engineering. This paper further delves into the manufacture of industrial products, such as artificial meats and bioinspired supramolecular architectures, and the mechanisms, physicochemical properties, and applications of peptide self-assembly. Undoubtedly, Israel contributes significantly to the field of 3D bioprinting and should thus be appropriately recognized.</p><h3 data-test=\"abstract-sub-heading\">Graphic abstract</h3>\u0000","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"5 1","pages":""},"PeriodicalIF":7.9,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140809288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spheroid construction strategies and application in 3D bioprinting 球体构建策略及在三维生物打印中的应用

IF 7.9 1区医学

Bio-Design and Manufacturing Pub Date : 2024-04-16 DOI: 10.1007/s42242-024-00273-7

Chunxiang Lu, Chuang Gao, Hao Qiao, Yi Zhang, Huazhen Liu, Aoxiang Jin, Yuanyuan Liu

{"title":"Spheroid construction strategies and application in 3D bioprinting","authors":"Chunxiang Lu, Chuang Gao, Hao Qiao, Yi Zhang, Huazhen Liu, Aoxiang Jin, Yuanyuan Liu","doi":"10.1007/s42242-024-00273-7","DOIUrl":"https://doi.org/10.1007/s42242-024-00273-7","url":null,"abstract":"<p>Tissue engineering has been striving toward designing and producing natural and functional human tissues. Cells are the fundamental building blocks of tissues. Compared with traditional two-dimensional cultured cells, cell spheres are three-dimensional (3D) structures that can naturally form complex cell–cell and cell–matrix interactions. This structure is close to the natural environment of cells in living organisms. In addition to being used in disease modeling and drug screening, spheroids have significant potential in tissue regeneration. The 3D bioprinting is an advanced biofabrication technique. It accurately deposits bioinks into predesigned 3D shapes to create complex tissue structures. Although 3D bioprinting is efficient, the time required for cells to develop into complex tissue structures can be lengthy. The 3D bioprinting of spheroids significantly reduces the time required for their development into large tissues/organs during later cultivation stages by printing them with high cell density. Combining spheroid fabrication and bioprinting technology should provide a new solution to many problems in regenerative medicine. This paper systematically elaborates and analyzes the spheroid fabrication methods and 3D bioprinting strategies by introducing spheroids as building blocks. Finally, we present the primary challenges faced by spheroid fabrication and 3D bioprinting with future requirements and some recommendations.</p><h3 data-test=\"abstract-sub-heading\">Graphic abstract</h3>","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"188 1","pages":""},"PeriodicalIF":7.9,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140614371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxygen tension modulates cell function in an in vitro three-dimensional glioblastoma tumor model 氧张力调节体外三维胶质母细胞瘤肿瘤模型中的细胞功能

IF 7.9 1区医学

Bio-Design and Manufacturing Pub Date : 2024-04-13 DOI: 10.1007/s42242-024-00271-9

Sen Wang, Siqi Yao, Na Pei, Luge Bai, Zhiyan Hao, Dichen Li, Jiankang He, J. Miguel Oliveira, Xiaoyan Xue, Ling Wang, Xinggang Mao

{"title":"Oxygen tension modulates cell function in an in vitro three-dimensional glioblastoma tumor model","authors":"Sen Wang, Siqi Yao, Na Pei, Luge Bai, Zhiyan Hao, Dichen Li, Jiankang He, J. Miguel Oliveira, Xiaoyan Xue, Ling Wang, Xinggang Mao","doi":"10.1007/s42242-024-00271-9","DOIUrl":"https://doi.org/10.1007/s42242-024-00271-9","url":null,"abstract":"<p>Hypoxia is a typical feature of the tumor microenvironment, one of the most critical factors affecting cell behavior and tumor progression. However, the lack of tumor models able to precisely emulate natural brain tumor tissue has impeded the study of the effects of hypoxia on the progression and growth of tumor cells. This study reports a three-dimensional (3D) brain tumor model obtained by encapsulating U87MG (U87) cells in a hydrogel containing type I collagen. It also documents the effect of various oxygen concentrations (1%, 7%, and 21%) in the culture environment on U87 cell morphology, proliferation, viability, cell cycle, apoptosis rate, and migration. Finally, it compares two-dimensional (2D) and 3D cultures. For comparison purposes, cells cultured in flat culture dishes were used as the control (2D model). Cells cultured in the 3D model proliferated more slowly but had a higher apoptosis rate and proportion of cells in the resting phase (G0 phase)/gap I phase (G1 phase) than those cultured in the 2D model. Besides, the two models yielded significantly different cell morphologies. Finally, hypoxia (e.g., 1% O<sub>2</sub>) affected cell morphology, slowed cell growth, reduced cell viability, and increased the apoptosis rate in the 3D model. These results indicate that the constructed 3D model is effective for investigating the effects of biological and chemical factors on cell morphology and function, and can be more representative of the tumor microenvironment than 2D culture systems. The developed 3D glioblastoma tumor model is equally applicable to other studies in pharmacology and pathology.</p><h3 data-test=\"abstract-sub-heading\">Graphic abstract</h3>\u0000","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"13 1","pages":""},"PeriodicalIF":7.9,"publicationDate":"2024-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140591571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ink-structing the future of vascular tissue engineering: a review of the physiological bioink design 墨水构建血管组织工程的未来：生理生物墨水设计综述

IF 7.9 1区医学

Bio-Design and Manufacturing Pub Date : 2024-03-07 DOI: 10.1007/s42242-024-00270-w

Judith Synofzik, Sebastian Heene, Rebecca Jonczyk, Cornelia Blume

{"title":"Ink-structing the future of vascular tissue engineering: a review of the physiological bioink design","authors":"Judith Synofzik, Sebastian Heene, Rebecca Jonczyk, Cornelia Blume","doi":"10.1007/s42242-024-00270-w","DOIUrl":"https://doi.org/10.1007/s42242-024-00270-w","url":null,"abstract":"<p>Three-dimensional (3D) printing and bioprinting have come into view for a plannable and standardizable generation of implantable tissue-engineered constructs that can substitute native tissues and organs. These tissue-engineered structures are intended to integrate with the patient’s body. Vascular tissue engineering (TE) is relevant in TE because it supports the sustained oxygenization and nutrition of all tissue-engineered constructs. Bioinks have a specific role, representing the necessary medium for printability and vascular cell growth. This review aims to understand the requirements for the design of vascular bioinks. First, an in-depth analysis of vascular cell interaction with their native environment must be gained. A physiological bioink suitable for a tissue-engineered vascular graft (TEVG) must not only ensure good printability but also induce cells to behave like in a native vascular vessel, including self-regenerative and growth functions. This review describes the general structure of vascular walls with wall-specific cell and extracellular matrix (ECM) components and biomechanical properties and functions. Furthermore, the physiological role of vascular ECM components for their interaction with vascular cells and the mode of interaction is introduced. Diverse currently available or imaginable bioinks are described from physiological matrix proteins to nonphysiologically occurring but natural chemical compounds useful for vascular bioprinting. The physiological performance of these bioinks is evaluated with regard to biomechanical properties postprinting, with a view to current animal studies of 3D printed vascular structures. Finally, the main challenges for further bioink development, suitable bioink components to create a self-assembly bioink concept, and future bioprinting strategies are outlined. These concepts are discussed in terms of their suitability to be part of a TEVG with a high potential for later clinical use.</p>","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"20 1","pages":""},"PeriodicalIF":7.9,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140055161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biofabrication of nanocomposite-based scaffolds containing human bone extracellular matrix for the differentiation of skeletal stem and progenitor cells 含有人骨细胞外基质的纳米复合材料支架的生物制造，用于骨骼干细胞和祖细胞的分化

IF 7.9 1区医学

Bio-Design and Manufacturing Pub Date : 2024-03-05 DOI: 10.1007/s42242-023-00265-z

Yang-Hee Kim, Janos M. Kanczler, Stuart Lanham, Andrew Rawlings, Marta Roldo, Gianluca Tozzi, Jonathan I. Dawson, Gianluca Cidonio, Richard O. C. Oreffo

{"title":"Biofabrication of nanocomposite-based scaffolds containing human bone extracellular matrix for the differentiation of skeletal stem and progenitor cells","authors":"Yang-Hee Kim, Janos M. Kanczler, Stuart Lanham, Andrew Rawlings, Marta Roldo, Gianluca Tozzi, Jonathan I. Dawson, Gianluca Cidonio, Richard O. C. Oreffo","doi":"10.1007/s42242-023-00265-z","DOIUrl":"https://doi.org/10.1007/s42242-023-00265-z","url":null,"abstract":"<p>Autograft or metal implants are routinely used in skeletal repair. However, they fail to provide long-term clinical resolution, necessitating a functional biomimetic tissue engineering alternative. The use of native human bone tissue for synthesizing a biomimetic material ink for three-dimensional (3D) bioprinting of skeletal tissue is an attractive strategy for tissue regeneration. Thus, human bone extracellular matrix (bone-ECM) offers an exciting potential for the development of an appropriate microenvironment for human bone marrow stromal cells (HBMSCs) to proliferate and differentiate along the osteogenic lineage. In this study, we engineered a novel material ink (LAB) by blending human bone-ECM (B) with nanoclay (L, Laponite<sup>®</sup>) and alginate (A) polymers using extrusion-based deposition. The inclusion of the nanofiller and polymeric material increased the rheology, printability, and drug retention properties and, critically, the preservation of HBMSCs viability upon printing. The composite of human bone-ECM-based 3D constructs containing vascular endothelial growth factor (VEGF) enhanced vascularization after implantation in an ex vivo chick chorioallantoic membrane (CAM) model. The inclusion of bone morphogenetic protein-2 (BMP-2) with the HBMSCs further enhanced vascularization and mineralization after only seven days. This study demonstrates the synergistic combination of nanoclay with biomimetic materials (alginate and bone-ECM) to support the formation of osteogenic tissue both in vitro and ex vivo and offers a promising novel 3D bioprinting approach to personalized skeletal tissue repair.</p><h3 data-test=\"abstract-sub-heading\">Graphic abstract</h3>\u0000","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"18 1","pages":""},"PeriodicalIF":7.9,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140035598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0