聚乳酸骨支架中的掺银 CNT/HAP 纳米杂化物显示出显著的抗菌活性

IF 8.1 1区 医学 Q1 ENGINEERING, BIOMEDICAL
Cijun Shuai, Xiaoxin Shi, Kai Wang, Yulong Gu, Feng Yang, Pei Feng
{"title":"聚乳酸骨支架中的掺银 CNT/HAP 纳米杂化物显示出显著的抗菌活性","authors":"Cijun Shuai, Xiaoxin Shi, Kai Wang, Yulong Gu, Feng Yang, Pei Feng","doi":"10.1007/s42242-023-00264-0","DOIUrl":null,"url":null,"abstract":"<p>Bacterial infection is a major problem following bone implant surgery. Moreover, poly-<span>l</span>-lactic acid/carbon nanotube/hydroxyapatite (PLLA/CNT/HAP) bone scaffolds possess enhanced mechanical properties and show good bioactivity regarding bone defect regeneration. In this study, we synthesized silver (Ag)-doped CNT/HAP (CNT/Ag-HAP) nanohybrids via the partial replacing of calcium ions (Ca<sup>2+</sup>) in the HAP lattice with silver ions (Ag<sup>+</sup>) using an ion doping technique under hydrothermal conditions. Specifically, the doping process was induced using the special lattice structure of HAP and the abundant surface oxygenic functional groups of CNT, and involved the partial replacement of Ca<sup>2+</sup> in the HAP lattice by doped Ag<sup>+</sup> as well as the in situ synthesis of Ag-HAP nanoparticles on CNT in a hydrothermal environment. The resulting CNT/Ag-HAP nanohybrids were then introduced into a PLLA matrix via laser-based powder bed fusion (PBF-LB) to fabricate PLLA/CNT/Ag-HAP scaffolds that showed sustained antibacterial activity. We then found that Ag<sup>+</sup>, which possesses broad-spectrum antibacterial activity, endowed PLLA/CNT/Ag-HAP scaffolds with this activity, with an antibacterial effectiveness of 92.65%. This antibacterial effect is due to the powerful effect of Ag<sup>+</sup> against bacterial structure and genetic material, as well as the physical destruction of bacterial structures due to the sharp edge structure of CNT. In addition, the scaffold possessed enhanced mechanical properties, showing tensile and compressive strengths of 8.49 MPa and 19.72 MPa, respectively. Finally, the scaffold also exhibited good bioactivity and cytocompatibility, including the ability to form apatite layers and to promote the adhesion and proliferation of human osteoblast-like cells (MG63 cells).</p><h3 data-test=\"abstract-sub-heading\">Graphic abstract</h3>","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"46 1","pages":""},"PeriodicalIF":8.1000,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Ag-doped CNT/HAP nanohybrids in a PLLA bone scaffold show significant antibacterial activity\",\"authors\":\"Cijun Shuai, Xiaoxin Shi, Kai Wang, Yulong Gu, Feng Yang, Pei Feng\",\"doi\":\"10.1007/s42242-023-00264-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Bacterial infection is a major problem following bone implant surgery. Moreover, poly-<span>l</span>-lactic acid/carbon nanotube/hydroxyapatite (PLLA/CNT/HAP) bone scaffolds possess enhanced mechanical properties and show good bioactivity regarding bone defect regeneration. In this study, we synthesized silver (Ag)-doped CNT/HAP (CNT/Ag-HAP) nanohybrids via the partial replacing of calcium ions (Ca<sup>2+</sup>) in the HAP lattice with silver ions (Ag<sup>+</sup>) using an ion doping technique under hydrothermal conditions. Specifically, the doping process was induced using the special lattice structure of HAP and the abundant surface oxygenic functional groups of CNT, and involved the partial replacement of Ca<sup>2+</sup> in the HAP lattice by doped Ag<sup>+</sup> as well as the in situ synthesis of Ag-HAP nanoparticles on CNT in a hydrothermal environment. The resulting CNT/Ag-HAP nanohybrids were then introduced into a PLLA matrix via laser-based powder bed fusion (PBF-LB) to fabricate PLLA/CNT/Ag-HAP scaffolds that showed sustained antibacterial activity. We then found that Ag<sup>+</sup>, which possesses broad-spectrum antibacterial activity, endowed PLLA/CNT/Ag-HAP scaffolds with this activity, with an antibacterial effectiveness of 92.65%. This antibacterial effect is due to the powerful effect of Ag<sup>+</sup> against bacterial structure and genetic material, as well as the physical destruction of bacterial structures due to the sharp edge structure of CNT. In addition, the scaffold possessed enhanced mechanical properties, showing tensile and compressive strengths of 8.49 MPa and 19.72 MPa, respectively. Finally, the scaffold also exhibited good bioactivity and cytocompatibility, including the ability to form apatite layers and to promote the adhesion and proliferation of human osteoblast-like cells (MG63 cells).</p><h3 data-test=\\\"abstract-sub-heading\\\">Graphic abstract</h3>\",\"PeriodicalId\":48627,\"journal\":{\"name\":\"Bio-Design and Manufacturing\",\"volume\":\"46 1\",\"pages\":\"\"},\"PeriodicalIF\":8.1000,\"publicationDate\":\"2024-02-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bio-Design and Manufacturing\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1007/s42242-023-00264-0\",\"RegionNum\":1,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, BIOMEDICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bio-Design and Manufacturing","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s42242-023-00264-0","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
引用次数: 0

摘要

细菌感染是骨植入手术后的一个主要问题。此外,聚乳酸/碳纳米管/羟基磷灰石(PLLA/CNT/HAP)骨支架具有更强的机械性能,并在骨缺损再生方面表现出良好的生物活性。在本研究中,我们在水热条件下采用离子掺杂技术,用银离子(Ag+)部分取代 HAP 晶格中的钙离子(Ca2+),合成了掺银(Ag)的 CNT/HAP(CNT/Ag-HAP)纳米混合物。具体来说,掺杂过程是利用 HAP 的特殊晶格结构和 CNT 丰富的表面含氧官能团诱导的,包括用掺杂的 Ag+ 部分取代 HAP 晶格中的 Ca2+,以及在水热环境下在 CNT 上原位合成 Ag-HAP 纳米粒子。然后,通过激光粉末床融合(PBF-LB)将生成的 CNT/Ag-HAP 纳米杂化物引入聚乳酸(PLLA)基质中,制备出具有持续抗菌活性的聚乳酸/CNT/Ag-HAP 支架。我们随后发现,具有广谱抗菌活性的 Ag+ 赋予了 PLLA/CNT/Ag-HAP 支架以这种活性,其抗菌效力高达 92.65%。这种抗菌效果得益于 Ag+ 对细菌结构和遗传物质的强大作用,以及 CNT 尖锐的边缘结构对细菌结构的物理破坏。此外,该支架还具有更强的机械性能,拉伸强度和压缩强度分别达到 8.49 兆帕和 19.72 兆帕。最后,该支架还表现出良好的生物活性和细胞相容性,包括形成磷灰石层的能力,以及促进人类成骨细胞(MG63 细胞)粘附和增殖的能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Ag-doped CNT/HAP nanohybrids in a PLLA bone scaffold show significant antibacterial activity

Ag-doped CNT/HAP nanohybrids in a PLLA bone scaffold show significant antibacterial activity

Bacterial infection is a major problem following bone implant surgery. Moreover, poly-l-lactic acid/carbon nanotube/hydroxyapatite (PLLA/CNT/HAP) bone scaffolds possess enhanced mechanical properties and show good bioactivity regarding bone defect regeneration. In this study, we synthesized silver (Ag)-doped CNT/HAP (CNT/Ag-HAP) nanohybrids via the partial replacing of calcium ions (Ca2+) in the HAP lattice with silver ions (Ag+) using an ion doping technique under hydrothermal conditions. Specifically, the doping process was induced using the special lattice structure of HAP and the abundant surface oxygenic functional groups of CNT, and involved the partial replacement of Ca2+ in the HAP lattice by doped Ag+ as well as the in situ synthesis of Ag-HAP nanoparticles on CNT in a hydrothermal environment. The resulting CNT/Ag-HAP nanohybrids were then introduced into a PLLA matrix via laser-based powder bed fusion (PBF-LB) to fabricate PLLA/CNT/Ag-HAP scaffolds that showed sustained antibacterial activity. We then found that Ag+, which possesses broad-spectrum antibacterial activity, endowed PLLA/CNT/Ag-HAP scaffolds with this activity, with an antibacterial effectiveness of 92.65%. This antibacterial effect is due to the powerful effect of Ag+ against bacterial structure and genetic material, as well as the physical destruction of bacterial structures due to the sharp edge structure of CNT. In addition, the scaffold possessed enhanced mechanical properties, showing tensile and compressive strengths of 8.49 MPa and 19.72 MPa, respectively. Finally, the scaffold also exhibited good bioactivity and cytocompatibility, including the ability to form apatite layers and to promote the adhesion and proliferation of human osteoblast-like cells (MG63 cells).

Graphic abstract

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Bio-Design and Manufacturing
Bio-Design and Manufacturing Materials Science-Materials Science (miscellaneous)
CiteScore
13.30
自引率
7.60%
发文量
148
期刊介绍: Bio-Design and Manufacturing reports new research, new technology and new applications in the field of biomanufacturing, especially 3D bioprinting. Topics of Bio-Design and Manufacturing cover tissue engineering, regenerative medicine, mechanical devices from the perspectives of materials, biology, medicine and mechanical engineering, with a focus on manufacturing science and technology to fulfil the requirement of bio-design.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信