Biosensors-Basel最新文献_第5页

Visualizing Macrophage Polarization through Fluorescent mRNA Profiling. 通过荧光 mRNA 图谱对巨噬细胞极化进行可视化分析

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2024-10-02 DOI: 10.3390/bios14100475

Miaomiao Xu, Siyuan Wei, Tong Su, Die Ma, Zhixuan Wang, Dan Zhu, Lixing Weng, Xianguang Ding

{"title":"Visualizing Macrophage Polarization through Fluorescent mRNA Profiling.","authors":"Miaomiao Xu, Siyuan Wei, Tong Su, Die Ma, Zhixuan Wang, Dan Zhu, Lixing Weng, Xianguang Ding","doi":"10.3390/bios14100475","DOIUrl":"https://doi.org/10.3390/bios14100475","url":null,"abstract":"Macrophages, known for their phenotypic plasticity, play a critical role in maintaining homeostasis and inflammation-related pathogenesis. Although identifying diverse macrophage phenotypes holds promise for enhancing diagnoses and treatments of diseases mediated by macrophages, existing methodologies for differentiating macrophages often lack precision. They are limited by the cumbersome procedures that require large-scale equipment, such as flow cytometry and transcriptomic analysis. In this context, we have engineered fluorescent polyadenine (polyA)-mediated sticky flares that enable practical visualization of macrophages. This technology facilitates the highly sensitive detection of macrophage phenotypes through the specific recognition of intracellular mRNAs, permitting in situ imaging. Our approach demonstrates the potential for determining macrophage polarization status at the single-cell level within dynamic immune microenvironments, thereby providing crucial diagnostic and prognostic information that could guide the development of tailored treatments for macrophage-related diseases in personalized medicine.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11506351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142510886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Nanoparticle-Coated Cellulose Acetate Membrane for Highly Efficient, Low-Cost Circulating Tumor Cell Detection. 一种用于高效、低成本检测循环肿瘤细胞的纳米颗粒包裹醋酸纤维素膜。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2024-10-01 DOI: 10.3390/bios14100472

Yize Zhao, Yaqi Pan, Hao Sun, Pengfei Huo, Guangtong Wang, Shaoqin Liu

{"title":"A Nanoparticle-Coated Cellulose Acetate Membrane for Highly Efficient, Low-Cost Circulating Tumor Cell Detection.","authors":"Yize Zhao, Yaqi Pan, Hao Sun, Pengfei Huo, Guangtong Wang, Shaoqin Liu","doi":"10.3390/bios14100472","DOIUrl":"https://doi.org/10.3390/bios14100472","url":null,"abstract":"Detecting circulating tumor cells has exhibited great significance in treating cancers since its concentration is an index strongly associated with the development and transfer of the tumor. However, the present commercial method for CTC detection is still expensive, because special antibodies and complicated devices must be used for cell separation and imaging. Hence, it is quite necessary to apply alternative materials and methods to decrease the cost of CTC detection. In this article, we coated a cellulose acetate membrane with nanoparticles formed by the polymerization of melamine and furfural, creating a surface with nanoscale roughness for the highly efficient capture of the sparse CTCs in a blood sample. Subsequently, the CTCs on the surface can be quantitatively detected by colorimetry with the aid of a COF-based nanozyme. The detection limit (LOD) can be as low as 3 cells/mL, which is the lowest LOD among the colorimetric methods to our knowledge. Considering the low cost of fabricating the membrane for CTC capture and the robustness of nanozymes compared with natural enzymes, this CTC detection approach displays great potential to decrease the financial burden of commercial CTC detection.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11505997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142510863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microscale Flow Control and Droplet Generation Using Arduino-Based Pneumatically-Controlled Microfluidic Device. 利用基于 Arduino 的气动控制微流控装置实现微尺度流量控制和液滴生成

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2024-09-30 DOI: 10.3390/bios14100469

Woohyun Park, Se-Woon Choe, Minseok Kim

引用次数: 0

Needle Tip Tracking through Photoluminescence for Minimally Invasive Surgery. 通过光致发光追踪针尖，用于微创手术。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2024-09-30 DOI: 10.3390/bios14100470

Meenakshi Narayan, Mithun Bhowmick

引用次数: 0

Aptamer-Mediated Electrochemical Detection of SARS-CoV-2 Nucleocapsid Protein in Saliva. 以肽聚体为媒介电化学检测唾液中的 SARS-CoV-2 核头壳蛋白

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2024-09-30 DOI: 10.3390/bios14100471

Ryan H P Siu, Robert G Jesky, Yu-Jing Fan, Cyrus C H Au-Yeung, Andrew B Kinghorn, Kwok-Hung Chan, Ivan Fan-Ngai Hung, Julian A Tanner

{"title":"Aptamer-Mediated Electrochemical Detection of SARS-CoV-2 Nucleocapsid Protein in Saliva.","authors":"Ryan H P Siu, Robert G Jesky, Yu-Jing Fan, Cyrus C H Au-Yeung, Andrew B Kinghorn, Kwok-Hung Chan, Ivan Fan-Ngai Hung, Julian A Tanner","doi":"10.3390/bios14100471","DOIUrl":"https://doi.org/10.3390/bios14100471","url":null,"abstract":"Gold standard detection of SARS-CoV-2 by reverse transcription quantitative PCR (RT-qPCR) can achieve ultrasensitive viral detection down to a few RNA copies per sample. Yet, the lengthy detection and labor-intensive protocol limit its effectiveness in community screening. In view of this, a structural switching electrochemical aptamer-based biosensor (E-AB) targeting the SARS-CoV-2 nucleocapsid (N) protein was developed. Four N protein-targeting aptamers were characterized on an electrochemical cell configuration using square wave voltammetry (SWV). The sensor was investigated in an artificial saliva matrix optimizing the aptamer anchoring orientation, SWV interrogation frequency, and target incubation time. Rapid detection of the N protein was achieved within 5 min at a low nanomolar limit of detection (LOD) with high specificity. Specific N protein detection was also achieved in simulated positive saliva samples, demonstrating its feasibility for saliva-based rapid diagnosis. Further research will incorporate novel signal amplification strategies to improve sensitivity for early diagnosis.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11505747/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142510800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Review of Advanced Sensor Technologies for Aquatic Products Freshness Assessment in Cold Chain Logistics. 用于冷链物流中水产品新鲜度评估的先进传感器技术综述。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2024-09-30 DOI: 10.3390/bios14100468

Baichuan Wang, Kang Liu, Guangfen Wei, Aixiang He, Weifu Kong, Xiaoshuan Zhang

{"title":"A Review of Advanced Sensor Technologies for Aquatic Products Freshness Assessment in Cold Chain Logistics.","authors":"Baichuan Wang, Kang Liu, Guangfen Wei, Aixiang He, Weifu Kong, Xiaoshuan Zhang","doi":"10.3390/bios14100468","DOIUrl":"https://doi.org/10.3390/bios14100468","url":null,"abstract":"The evaluation of the upkeep and freshness of aquatic products within the cold chain is crucial due to their perishable nature, which can significantly impact both quality and safety. Conventional methods for assessing freshness in the cold chain have inherent limitations regarding specificity and accuracy, often requiring substantial time and effort. Recently, advanced sensor technologies have been developed for freshness assessment, enabling real-time and non-invasive monitoring via the detection of volatile organic compounds, biochemical markers, and physical properties. The integration of sensor technologies into cold chain logistics enhances the ability to maintain the quality and safety of aquatic products. This review examines the advancements made in multifunctional sensor devices for the freshness assessment of aquatic products in cold chain logistics, as well as the application of pattern recognition algorithms for identification and classification. It begins by outlining the categories of freshness criteria, followed by an exploration of the development of four key sensor devices: electronic noses, electronic tongues, biosensors, and flexible sensors. Furthermore, the review discusses the implementation of advanced pattern recognition algorithms in sensor devices for freshness detection and evaluation. It highlights the current status and future potential of sensor technologies for aquatic products within the cold chain, while also addressing the significant challenges that remain to be overcome.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11506179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142510864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effective Boundary Correction for Deterministic Lateral Displacement Microchannels to Improve Cell Separation: A Numerical and Experimental Study. 确定性侧向位移微通道的有效边界校正以改善细胞分离：数值和实验研究。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2024-09-29 DOI: 10.3390/bios14100466

Shaghayegh Mirhosseini, Mohammadmahdi Eskandarisani, Aryanaz Faghih Nasiri, Fatemeh Khatami, Akram Mirzaei, Majid Badieirostami, Seyed Mohammad Kazem Aghamir, Mohammadreza Kolahdouz

{"title":"Effective Boundary Correction for Deterministic Lateral Displacement Microchannels to Improve Cell Separation: A Numerical and Experimental Study.","authors":"Shaghayegh Mirhosseini, Mohammadmahdi Eskandarisani, Aryanaz Faghih Nasiri, Fatemeh Khatami, Akram Mirzaei, Majid Badieirostami, Seyed Mohammad Kazem Aghamir, Mohammadreza Kolahdouz","doi":"10.3390/bios14100466","DOIUrl":"https://doi.org/10.3390/bios14100466","url":null,"abstract":"Particle separation and sorting techniques based on microfluidics have found extensive applications and are increasingly gaining prominence. This research presents the design and fabrication of a microfluidic device for separating cells using deterministic lateral displacement (DLD), enabling accuracy and continuity while being size-based. Nevertheless, it remains demanding, to completely reverse the detrimental effects of the boundaries that disturb the fluidic flow in the channel and reduce particle separation efficiency. This study introduces a novel approach to enhance the boundary structure of channels. By using this design, separation efficiency is boosted, and the fluid behavior around the walls is improved. The boundary correction (BC) enhances the operation of the microchannel and is very effective in microchannels. With boundary correction, the device exhibited improved separation efficiencies, but in its absence, separation efficiencies dropped. The collected microscopic images of the isolation of prostate cancer cell lines and red blood cells revealed promising outcomes. The efficiency of circulating tumor cell (CTC) throughput in the microfluidic channel, quantified as the ratio or proportion of tumor cells exiting the channel to cells entering it, exceeds 93%. Moreover, the efficiency of CTC isolation, expressed as the proportion of tumor cells from the upper outlet of the microfluidic channel to all cells, is over 89%. Additionally, the efficiency of red blood cell isolation, evaluated as the ratio of red blood cells from the lower outlet of the microfluidic channel to all cells, surpasses 77%. While using the same DLD separator without boundary correction reduced the separation efficiency by around 5%.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11506467/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142510806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distinguishing of Histopathological Staging Features of H-E Stained Human cSCC by Microscopical Multispectral Imaging. 通过显微多光谱成像区分 H-E 染色人 cSCC 的组织病理学分期特征

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2024-09-29 DOI: 10.3390/bios14100467

Rujuan Wu, Jiayi Yang, Qi Chen, Changxing Yang, Qianqian Ge, Danni Rui, Huazhong Xiang, Dawei Zhang, Cheng Wang, Xiaoqing Zhao

{"title":"Distinguishing of Histopathological Staging Features of H-E Stained Human cSCC by Microscopical Multispectral Imaging.","authors":"Rujuan Wu, Jiayi Yang, Qi Chen, Changxing Yang, Qianqian Ge, Danni Rui, Huazhong Xiang, Dawei Zhang, Cheng Wang, Xiaoqing Zhao","doi":"10.3390/bios14100467","DOIUrl":"https://doi.org/10.3390/bios14100467","url":null,"abstract":"Cutaneous squamous cell carcinoma (cSCC) is the second most common malignant skin tumor. Early and precise diagnosis of tumor staging is crucial for long-term outcomes. While pathological diagnosis has traditionally served as the gold standard, the assessment of differentiation levels heavily depends on subjective judgments. Therefore, how to improve the diagnosis accuracy and objectivity of pathologists has become an urgent problem to be solved. We used multispectral imaging (MSI) to enhance tumor classification. The hematoxylin and eosin (H&E) stained cSCC slides were from Shanghai Ruijin Hospital. Scale-invariant feature transform was applied to multispectral images for image stitching, while the adaptive threshold segmentation method and random forest segmentation method were used for image segmentation, respectively. Synthetic pseudo-color images effectively highlight tissue differences. Quantitative analysis confirms significant variation in the nuclear area between normal and cSCC tissues (p < 0.001), supported by an AUC of 1 in ROC analysis. The AUC within cSCC tissues is 0.57. Further study shows higher nuclear atypia in poorly differentiated cSCC tissues compared to well-differentiated cSCC (p < 0.001), also with an AUC of 1. Lastly, well differentiated cSCC tissues show more and larger keratin pearls. These results have shown that combined MSI with imaging processing techniques will improve H&E stained human cSCC diagnosis accuracy, and it will be well utilized to distinguish histopathological staging features.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11506349/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142510805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in Genotyping Detection of Fragmented Nucleic Acids. 片段核酸基因分型检测的进展。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2024-09-28 DOI: 10.3390/bios14100465

Qian Liu, Yun Chen, Hao Qi

引用次数: 0

Portable Electrochemical System and Platform with Point-of-Care Determination of Urine Albumin-to-Creatinine Ratio to Evaluate Chronic Kidney Disease and Cardiorenal Syndrome. 便携式电化学系统和平台，可在护理点测定尿白蛋白与肌酐比值，以评估慢性肾病和心肾综合征。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2024-09-27 DOI: 10.3390/bios14100463

Shuenn-Yuh Lee, Ding-Siang Ciou, Hao-Yun Lee, Ju-Yi Chen, Yi-Chieh Wei, Meng-Dar Shieh

{"title":"Portable Electrochemical System and Platform with Point-of-Care Determination of Urine Albumin-to-Creatinine Ratio to Evaluate Chronic Kidney Disease and Cardiorenal Syndrome.","authors":"Shuenn-Yuh Lee, Ding-Siang Ciou, Hao-Yun Lee, Ju-Yi Chen, Yi-Chieh Wei, Meng-Dar Shieh","doi":"10.3390/bios14100463","DOIUrl":"https://doi.org/10.3390/bios14100463","url":null,"abstract":" The urine albumin (Alb)-to-creatinine (Crn) ratio (UACR) is a sensitive and early indicator of chronic kidney disease (CKD) and cardiorenal syndrome. This study developed a portable and wireless electrochemical-sensing platform for the sensitive and accurate determination of UACR. The developed platform consists of a carbon nanotube (CNT)-2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)(ABTS)-based modified UACR sensor, a miniaturised potentiostat, a cup holder embedded with a magnetic stirrer and a smartphone app. The UACR sensing electrode is composed of two screen-printed carbon working electrodes, one screen-printed carbon counter electrode and a screen-printed AgCl reference electrode. The miniaturised potentiostat, which is controlled by the developed app, performs cyclic voltammetry and amperometry to detect Alb and Crn, respectively. Clinical trials of the proposed system by using spot urine samples from 30 diabetic patients indicate that it can accurately classify all three CKD risk statuses within 30 min. The high accuracy of our proposed sensing system exhibits satisfactory agreement with the commercial biochemical analyser TBA-25FR (Y = 0.999X, R2 = 0.995). The proposed UACR sensing system offers a convenient, reliable and affordable solution for personal mobile health monitoring and point-of-care urinalysis.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11506532/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142510850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0