Biosensors-Basel最新文献_第3页

Advances of Nanozyme-Driven Multimodal Sensing Strategies in Point-of-Care Testing. 纳米酶驱动的多模态传感策略在护理点检测中的进展。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-06-10 DOI: 10.3390/bios15060375

Ziyi Chang, Qingjie Fu, Mengke Wang, Demin Duan

{"title":"Advances of Nanozyme-Driven Multimodal Sensing Strategies in Point-of-Care Testing.","authors":"Ziyi Chang, Qingjie Fu, Mengke Wang, Demin Duan","doi":"10.3390/bios15060375","DOIUrl":"10.3390/bios15060375","url":null,"abstract":"Point-of-care testing (POCT) has garnered widespread attention due to its rapid, convenient, and efficient detection capabilities, particularly playing an increasingly pivotal role in medical diagnostics and significantly improving the efficiency and quality of healthcare services. Nanozymes, as novel enzyme-mimicking materials, have emerged as a research hotspot owing to their superior catalytic performance, low cost, and robust stability. This review provides a systematic overview of the fundamental characteristics and classifications of nanozymes, along with various sensing strategies employed in POCT applications, colorimetric, electrochemical, fluorescent, chemiluminescent, and surface-enhanced Raman scattering (SERS)-based approaches. Furthermore, this review highlights innovative designs that enhance the sensitivity and accuracy of POCT across multiple domains, such as biomarker detection, environmental monitoring, and food safety analysis, thereby offering novel perspectives for the practical implementation of nanozymes in point-of-care diagnostics. Finally, this review analyzes current challenges in nanozyme-based POCT systems, including limitations in optimizing catalytic activity, ensuring nanozyme homogeneity, and achieving large-scale production, while proposing future development trajectories.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 6","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12190383/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing the Role of EEG Biosignal Preprocessing to Enhance Multiscale Fuzzy Entropy in Alzheimer's Disease Detection. 评价脑电信号预处理增强多尺度模糊熵在阿尔茨海默病检测中的作用。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-06-10 DOI: 10.3390/bios15060374

Pasquale Arpaia, Maria Cacciapuoti, Andrea Cataldo, Sabatina Criscuolo, Egidio De Benedetto, Antonio Masciullo, Marisa Pesola, Raissa Schiavoni

{"title":"Assessing the Role of EEG Biosignal Preprocessing to Enhance Multiscale Fuzzy Entropy in Alzheimer's Disease Detection.","authors":"Pasquale Arpaia, Maria Cacciapuoti, Andrea Cataldo, Sabatina Criscuolo, Egidio De Benedetto, Antonio Masciullo, Marisa Pesola, Raissa Schiavoni","doi":"10.3390/bios15060374","DOIUrl":"10.3390/bios15060374","url":null,"abstract":"Quantitative electroencephalography (QEEG) has emerged as a promising tool for detecting Alzheimer's disease (AD). Among QEEG measures, Multiscale Fuzzy Entropy (MFE) shows great potential in identifying AD-related changes in EEG complexity. However, MFE is intrinsically linked to signal amplitude, which can vary substantially among EEG systems, and this hinders the adoption of this metric for AD detection. To overcome this issue, this study investigates different preprocessing strategies to make the calculation of MFE less dependent on the specific amplitude characteristics of the EEG signals at hand. This contributes to generalizing and making more robust the adoption of MFE for AD detection. To demonstrate the robustness of the proposed preprocessing methods, binary classification tasks with Support Vector Machines (SVMs), Random Forest (RF), and K-Nearest Neighbor (KNN) classifiers are used. Performance metrics, such as classification accuracy and Matthews Correlation Coefficient (MCC), are employed to assess the results. The methodology is validated on two public EEG datasets. Results show that amplitude transformation, particularly normalization, significantly enhances AD detection, achieving mean classification accuracy values exceeding 80% with an uncertainty of 10% across all classifiers. These results highlight the importance of preprocessing in improving the accuracy and the reliability of EEG-based AD diagnostic tools, offering potential advancements in patient management and treatment planning.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 6","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12190283/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological Sensing Using Vertical MoS₂-Graphene Heterostructure-Based Field-Effect Transistor Biosensors. 基于垂直mos2 -石墨烯异质结构场效应晶体管生物传感器的生物传感。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-06-10 DOI: 10.3390/bios15060373

Ying Chen, Nataly Vicente, Tung Pham, Ashok Mulchandani

{"title":"Biological Sensing Using Vertical MoS2-Graphene Heterostructure-Based Field-Effect Transistor Biosensors.","authors":"Ying Chen, Nataly Vicente, Tung Pham, Ashok Mulchandani","doi":"10.3390/bios15060373","DOIUrl":"10.3390/bios15060373","url":null,"abstract":"Our study develops two configurations of MoS2 and graphene heterostructures-MoS2 on graphene (MG) and graphene on MoS2 (GM)-to investigate biomolecule sensing in field-effect transistor (FET) biosensors. Leveraging MoS2 and graphene's distinctive properties, we employ specialized functionalization techniques for each configuration: graphene with MoS2 on top uses a silane-based method with triethoxysilylbutyraldehyde (TESBA), and MoS2 with graphene on top utilizes 1-pyrenebutyric acid N-hydroxysuccinimide ester (PBASE). Our research explores the application of MoS2-Graphene heterostructures in biosensors, emphasizing the roles of synthesis, fabrication, and material functionalization in optimizing sensor performance. Through our experimental investigations, we have observed that doping MoS2 and graphene leads to noticeable changes in the Raman spectrum and shifts in transfer curves. Techniques such as XPS, Raman, and AFM have successfully confirmed the biofunctionalization. Transfer curves were instrumental in characterizing the biosensing performance, revealing that GM configurations exhibit higher sensitivity and a lower limit of detection (LOD) compared to MG configurations. We demonstrate that GM heterostructures offer superior sensitivity and specificity in biosensing, highlighting their significant potential to advance biosensor technologies. This research contributes to the field by detailing the creation process of vertical MoS2-graphene heterostructures and evaluating their effectiveness in accurate biomolecule detection, advancing biosensing technology.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 6","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12190709/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in MoS₂-Based Biosensors: From Material Fabrication and Characterization to Biomedical, Environmental, and Industrial Applications. 基于二硫化钼的生物传感器的进展：从材料制造和表征到生物医学，环境和工业应用。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-06-10 DOI: 10.3390/bios15060371

Chun-Liang Lai, Arvind Mukundan, Riya Karmakar, Roopmeet Kaur, Kuo-Liang Huang, Hsiang-Chen Wang

{"title":"Advances in MoS2-Based Biosensors: From Material Fabrication and Characterization to Biomedical, Environmental, and Industrial Applications.","authors":"Chun-Liang Lai, Arvind Mukundan, Riya Karmakar, Roopmeet Kaur, Kuo-Liang Huang, Hsiang-Chen Wang","doi":"10.3390/bios15060371","DOIUrl":"10.3390/bios15060371","url":null,"abstract":"The growing demand for low-cost biosensors has stimulated the study of new technologies and materials like molybdenum disulfide (MoS2). Due to its electroconductive nature and high surface-to-volume ratio, it allows for the ultra-sensitive detection of biomarkers. The crystal structure of MoS2 provides it with a unique micrometer thickness, making it appropriate for biosensing in healthcare, environmental monitoring, and food safety. As compared to traditional materials, MoS2 can work without labels (through field-effect transduction or plasmonic shifts) while maintaining biocompatibility and low-cost fabrication, which fill significant voids in the early diagnosis of diseases. This paper provides an overview of the recent advancements in MoS2-based biosensors, which are primarily focused on the field-effect transistors and surface plasmon resonance techniques and fabrication methods for MoS2-based biosensors like mechanical exfoliation, liquid-phase exfoliation, physical vapor deposition, chemical vapor deposition, and chemical exfoliation, applications in various industries, and their characterization techniques to evaluate the quality and functionality of MoS2 nanosheets in biosensors. While certain challenges remain like improving conductivity and scalability, MoS2-based biosensors serve as a powerful tool for the precise and reliable detection of biomarkers in environmental, food, and healthcare industries.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 6","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12190200/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sudomotor Dysfunction as an Early Marker of Autonomic and Cardiovascular Risk in Diabetes: Insights from a Cross-Sectional Study Using SUDOSCAN. Sudomotor功能障碍是糖尿病自主神经和心血管风险的早期标志：来自SUDOSCAN横断面研究的见解

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-06-10 DOI: 10.3390/bios15060372

Larisa Anghel, Claudiu Cobuz, Laura-Cătălina Benchea, Vasile Maciuc, Maricela Cobuz, Radu-Andy Sascău, Cristian Stătescu

{"title":"Sudomotor Dysfunction as an Early Marker of Autonomic and Cardiovascular Risk in Diabetes: Insights from a Cross-Sectional Study Using SUDOSCAN.","authors":"Larisa Anghel, Claudiu Cobuz, Laura-Cătălina Benchea, Vasile Maciuc, Maricela Cobuz, Radu-Andy Sascău, Cristian Stătescu","doi":"10.3390/bios15060372","DOIUrl":"10.3390/bios15060372","url":null,"abstract":"Background: Diabetic neuropathy, particularly in its autonomic form, is often underdiagnosed despite its clinical significance. Electrochemical skin conductance (ESC), measured by SUDOSCAN, offers a non-invasive way to assess the autonomic dysfunction.Methods: A total of 288 diabetic patients were assessed using SUDOSCAN to measure ESC in the hands and feet. Clinical and laboratory parameters, including glycated hemoglobin (HbA1c), body mass index (BMI), blood pressure, lipid profile, and cardiovascular risk, were analyzed for correlations with ESC. Neuropathy status was evaluated, and ROC analysis was performed to assess diagnostic accuracy.Results: Sudomotor dysfunction was prevalent, particularly in patients with a diabetes duration exceeding 20 years (p < 0.05). Men showed significantly higher right foot ESC than women (76.5 ± 13.1 vs. 74.0 ± 13.5 µS, p = 0.041). A strong inverse correlation was found between cardiovascular risk score and right foot ESC (r = -0.455, p < 0.001). Left foot ESC also correlated inversely with cardiovascular risk (r = -0.401, p < 0.001) and HbA1c (r = -0.150, p = 0.049), while a weak positive correlation was seen with BMI (r = 0.145, p = 0.043). ROC analysis showed the highest area under the curve (AUC) in right foot ESC for autonomic neuropathy (AUC = 0.750, 95% CI: 0.623-0.877, p < 0.001).Conclusions: This study is among the few to systematically correlate ESC with validated cardiovascular risk scores in a diabetic outpatient cohort, highlighting its potential as a novel early screening biomarker for autonomic and cardiovascular complications.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 6","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12191207/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving the Enrichment of Submicron-Sized Particles by Size Decreasing of Cruciform Cross-Sectional Microchannel in Viscoelastic Microfluidics. 粘弹性微流体中减小十字截面微通道对亚微米颗粒富集的影响。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-06-09 DOI: 10.3390/bios15060370

Jaekyeong Jang, Eunjin Kim, Sungdong Kim, Ok-Chan Jeong, Sangwook Lee, Younghak Cho

{"title":"Improving the Enrichment of Submicron-Sized Particles by Size Decreasing of Cruciform Cross-Sectional Microchannel in Viscoelastic Microfluidics.","authors":"Jaekyeong Jang, Eunjin Kim, Sungdong Kim, Ok-Chan Jeong, Sangwook Lee, Younghak Cho","doi":"10.3390/bios15060370","DOIUrl":"10.3390/bios15060370","url":null,"abstract":"The manipulation of cells and bioparticles has garnered significant interest in the field of viscoelastic microfluidics, particularly regarding its capacity for single-stream focusing within a three-dimensional and simple microchannel structure. The inherent simplicity of this method enables the effective manipulation of particles, facilitating the separation and focusing of various cell types, including blood cells, circulating tumor cells (CTCs), and microalgae. However, the viscoelastic nature of the particles imposes limitations in the handling of submicron-sized particles, due to a significant decrease in the viscoelastic force acting on the particle. In this study, we propose a microfluidic device featuring a cruciform cross-sectional microchannel with 45 µm and 45 µm of its vertical and horizontal size, respectively. The cruciform microchannel, which has a 270° reflex angle on four corners, can increase the viscoelastic force on the particles, allowing the device to focus submicron-sized particles down to 180 nm in a single-stream manner. It is important to note that the single-stream formation was maintained, while the channel width at the outlet region was drastically increased, allowing for the enrichment of submicron-sized particles. For biological feasibility, the proposed device also demonstrates the single-stream focusing on biological particles such as bacteria. The presented microfluidic device would have great potential for the focusing and enrichment of nanoparticles including bacteria in a highly robust manner, expecting its use in the various fields such as diverse biological analysis and biomedical research.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 6","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12191125/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Large-Area Nanogap Platforms for Surface-Enhanced Raman Spectroscopy Toward Sensing Applications: Comparison Between Ag and Au. 面向传感应用的表面增强拉曼光谱大面积纳米间隙平台：银和金之间的比较。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-06-09 DOI: 10.3390/bios15060369

Arunkumar Alagurasu, Satyabrat Behera, Joon-Mo Yang, Dai-Sik Kim, Seon Namgung

{"title":"Large-Area Nanogap Platforms for Surface-Enhanced Raman Spectroscopy Toward Sensing Applications: Comparison Between Ag and Au.","authors":"Arunkumar Alagurasu, Satyabrat Behera, Joon-Mo Yang, Dai-Sik Kim, Seon Namgung","doi":"10.3390/bios15060369","DOIUrl":"10.3390/bios15060369","url":null,"abstract":"Sub-wavelength metallic nanostructures allow the squeezing of light within nanoscale regions, called plasmonic hotspots. Squeezed near-field light has been demonstrated to detect, modulate, and generate light in more effective ways. The enhanced electric field in the plasmonic hotspots are also utilized for identifying molecular fingerprints in a more sensitive manner, i.e., surface-enhanced Raman spectroscopy (SERS). SERS is a versatile tool used to characterize chemicals and biomolecules with the advantages of label-free detection, specificity, and high sensitivity compared to fluorescence and colorimetric sensing methods. With its practical and diverse applications such as biomedical sensing, the evaluation of SERS on diverse nano-structure platforms and materials is highly in demand. Nanogap structures are promising SERS platforms which can be fabricated over a large area with uniform nanoscale gap size. Here, we demonstrate the fabrication of large-area metal-insulator-metal nanogap structures with different metals (i.e., Au and Ag) and analyze material dependence on SERS. While both nanometer-sized gap structures exhibit a large enhancement factor for Raman spectroscopy, Ag-based structures exhibit 58- and 15-times-larger enhancement factors for bottom and top plasmonic hotspots, respectively. The enhanced detection on a silver nanogap platform is attributed to enhanced electric field in the gap, as confirmed by simulation. Our findings provide not only a way to better understand SERS in different metallic nano platforms but also insights for designing highly sensitive nanoscale chemical and biomedical sensors.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 6","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12191043/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-Mode Coupling Enabled Broadband Coverage for Terahertz Biosensing Applications. 太赫兹生物传感应用的多模耦合宽带覆盖。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-06-07 DOI: 10.3390/bios15060368

Dongyu Hu, Mengya Pan, Yanpeng Shi, Yifei Zhang

{"title":"Multi-Mode Coupling Enabled Broadband Coverage for Terahertz Biosensing Applications.","authors":"Dongyu Hu, Mengya Pan, Yanpeng Shi, Yifei Zhang","doi":"10.3390/bios15060368","DOIUrl":"10.3390/bios15060368","url":null,"abstract":"Terahertz (THz) biosensing faces critical challenges in balancing high sensitivity and broadband spectral coverage, particularly under miniaturized device constraints. Conventional quasi-bound states in the continuum (QBIC) metasurfaces achieve high quality factor (Q) but suffer from narrow bandwidth, while angle-scanning strategies for broadband detection require complex large-angle illumination. Here, we propose a symmetry-engineered, all-dielectric metasurface that leverages multipolar interference coupling to overcome this limitation. By introducing angular perturbation, the metasurface transforms the original magnetic dipole (MD)-dominated QBIC resonance into hybridized, multipolar modes. It arises from the interference coupling between MD, toroidal dipole (TD), and magnetic quadrupole (MQ). This mechanism induces dual counter-directional, frequency-shifted, resonance branches within angular variations below 16°, achieving simultaneous 0.42 THz broadband coverage and high Q of 499. Furthermore, a derived analytical model based on Maxwell equations and mode coupling theory rigorously validates the linear relationship between frequency splitting interval and incident angle with the Relative Root Mean Square Error (RRMSE) of 1.4% and the coefficient of determination (R2) of 0.99. This work establishes a paradigm for miniaturized THz biosensors, advancing applications in practical molecular diagnostics and multi-analyte screening.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 6","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12190500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical Detection of Prostate Cancer-Associated miRNA-141 Using a Low-Cost Disposable Biosensor. 低成本一次性生物传感器对前列腺癌相关miRNA-141的电化学检测

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-06-06 DOI: 10.3390/bios15060364

Alexander Hunt, Gymama Slaughter

{"title":"Electrochemical Detection of Prostate Cancer-Associated miRNA-141 Using a Low-Cost Disposable Biosensor.","authors":"Alexander Hunt, Gymama Slaughter","doi":"10.3390/bios15060364","DOIUrl":"10.3390/bios15060364","url":null,"abstract":"Prostate cancer is the second leading cause of cancer-related deaths among men in the United States. The early detection of aggressive forms is critical. Current diagnostic methods, including PSA testing and biopsies, are invasive and often yield false results. MicroRNA-141 (miRNA-141) has emerged as a promising non-invasive biomarker due to its elevated levels in the urine of patients with metastatic prostate cancer. Here, a low-cost, paper-based electrochemical biosensor for the sensitive detection of miRNA-141 in synthetic urine is reported. The device employs inkjet-printed gold electrodes on photopaper, functionalized with thiolated single-stranded DNA-141 capture probes for specific target recognition. The biosensor achieves a sensitivity of 78.66 fM µA-1 cm-2 and a linear detection range of 1 fM to 100 nM, encompassing clinically relevant concentrations of miRNA-141 found in patients with metastatic prostate cancer. A low limit of detection of 2.15 fM, strong selectivity against non-target sequences, and a rapid response time of 15 min further highlight the diagnostic potential of the device. This platform represents a significant advancement in the development of point-of-care diagnostic tools for prostate cancer and is readily adaptable for detecting other disease-specific miRNAs through simple probe modification. As such, it holds broad promise for accessible, early-stage cancer detection and longitudinal disease monitoring in diverse clinical settings.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 6","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12190380/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of Gait Speed on Inter-Joint Coordination in People with and Without Parkinson's Disease. 步态速度对帕金森病患者关节间协调性的影响。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-06-06 DOI: 10.3390/bios15060367

Patrick Ippersiel, Philippe C Dixon, Elke Warmerdam, Robbin Romijnders, Walter Maetzler, Clint Hansen

{"title":"Influence of Gait Speed on Inter-Joint Coordination in People with and Without Parkinson's Disease.","authors":"Patrick Ippersiel, Philippe C Dixon, Elke Warmerdam, Robbin Romijnders, Walter Maetzler, Clint Hansen","doi":"10.3390/bios15060367","DOIUrl":"10.3390/bios15060367","url":null,"abstract":"Background: The influence of gait speed on lower-extremity coordination while walking in people with Parkinson's disease (pwPD) is poorly understood. This study sought to investigate the relationship between gait speed and hip-knee coordination and coordination variability in older adults and pwPD.Methods: A total of 27 pwPD and 21 healthy older adults were recruited. Participants walked in a straight line at slow, preferred, and fast walking speeds. Gait data were collected using inertial measurement units, and the kinematics of the hip and knee were calculated. Coordination and coordination variability at the hip-knee joint pair were determined using continuous relative phase. A repeated measures two-way ANCOVA tested the impact of gait speed on coordination and coordination variability, while group differences were evaluated using statistical parametric mapping (SPM).Results: Neither the healthy older adults nor the pwPD adjusted their hip-knee coordination in response to changes in gait speed. pwPD also displayed a trend towards restricted hip and knee joint excursion compared to older adults, which may further limit their ability to adapt gait strategies.Conclusions: These findings suggest that interventions addressing both joint excursion and motor adaptability may be important for improving gait function in individuals with Parkinson's disease. Real-world applicability can be found in the potential of wearable sensors to become a valuable tool in routine clinical practice for both diagnosis and ongoing management.Trial registration: The study is registered in the German Clinical Trials Register (DRKS00022998).","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 6","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12190309/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0