Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry最新文献

{"title":"Geodorum densiflorum Rhizome Lectin Induces Apoptosis in Human Lung Cancer Cells and Inhibits the Growth of Ehrlich Ascites Carcinoma Cells In Vivo in Mice","authors":"Mizanur R. Mamun,&nbsp;Syed R. Kabir,&nbsp;Imtiaj Hasan,&nbsp;A. K. M. Asaduzzaman,&nbsp;Mohammad T. Alam","doi":"10.1134/S1990750825600116","DOIUrl":"10.1134/S1990750825600116","url":null,"abstract":"<p><i>Geodoram densiflorum</i> or pink nodding orchid grows in wet habitats in Bangladesh. Rural people traditionally use this orchid’s pseudobulbs to treat skin inflammation, tumors and carbuncles. Though a protein from these bulbs previously showed antitumor activity against Ehrlich ascites carcinoma (EAC) cells in vitro, this study focused on proving this effect in vivo in a mice model and against a human cancer cell line. GDL exhibited around 45% growth inhibition against A549 cells at a concentration of 64 µg/mL implicating a caspase-dependent pathway modulated by caspase-3, -8, and -9 inhibitors. The condensed nuclei and morphological changes of GDL-treated cells indicating apoptosis also became evident by Hoechst 33342 and FITC-annexin V/PI staining. GDL up-regulated CASP9 and p53 while down-regulating PARP, MAPK, and STAT3 gene expression, suggesting the involvement of death receptor and intrinsic mitochondrial pathways. Flow cytometry analysis revealed GDL-induced G₀/G₁ phase cell cycle arrest. In vivo, GDL inhibited EAC cell proliferation by 48%, extending EAC-bearing mice lifespan by 37.05%, and improved hematological parameters, notably increasing red blood cell counts and hemoglobin levels to 2.41 × 10⁹ RBC/mL and 10.27 g/dL, respectively. These findings underscore the therapeutic potential of GDL, setting the stage for further in-depth research.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"19 2","pages":"199 - 209"},"PeriodicalIF":0.4,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145144393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Ornithine Decarboxylase and Ferric Reducing Capacity Levels as Potential Biomarkers for Polycystic Ovary Syndrome","authors":"Noor Jasim Mohamed,&nbsp; Ali Waleed Al-Ani","doi":"10.1134/S1990750825600220","DOIUrl":"10.1134/S1990750825600220","url":null,"abstract":"<p>Background: Polycystic ovary syndrome (PCOS) is a prevalent hormonal disorder affecting reproductive-age women, often linked to metabolic issues like insulin resistance. Objective: this study aimed to evaluate ornithine decarboxylase (ODC) and ferric reducing capacity (FRC) levels in women with PCOS, with assess the effects of metformin and Primolut N treatment on their levels. Subjects and Methods: A case−control study was conducted with 150 married Iraqi women, categorized into three groups: 50 healthy controls, 50 untreated PCOS, 50 treated PCOS. Blood samples were analyzed for ODC, FRC levels and hormonal profiles. Statistical analysis applied independent <i>t</i>-test, Pearson’s correlation, ROC curve. Results: The ODC level was significantly increased while the FRC level was substantially decreased in PCOS patients compared to controls. Under metformin and Primolut N treatment, both ODC and FRC concentrations were improved to be close to their levels in control group. This reveals the potential role of ODC and FRC in PCOS progression. ROC results showed perfect sensitivity and specificity of ODC and FRC in PCOS diagnosing. Conclusions: The study introduces ODC and FRC as a valuable biomarker for diagnosing PCOS, highlighting its role in the disorder’s pathophysiology.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"19 2","pages":"145 - 154"},"PeriodicalIF":0.4,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145144400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variability in the Content of 8-Hydroxy-2-deoxyguanosine as a Marker of Oxidative Stress during Subchronic Intoxication with Pesticides","authors":"V. A. Korolev,&nbsp;L. A. Babkina,&nbsp;E. V. Felker,&nbsp;L. A. Yachmeneva,&nbsp;R. Yu. Chertova,&nbsp;M. A. Usachev,&nbsp;A. I. Milova","doi":"10.1134/S1990750824601000","DOIUrl":"10.1134/S1990750824601000","url":null,"abstract":"<p>In this study, a change in the concentration of 8-hydroxy-2-deoxyguanosine (8-OHdG) in the oral fluid and plasma of model animals (rats) during subchronic intoxication with different classes of pesticides (thiram and imidacloprid) was studied. The intake of pesticides into the organism of rats with standard feed or water at a dose of 1/50 LD₅₀ for 28 days is accompanied by the production of an excess amount of reactive oxygen species (ROS) in the oral fluid and plasma. A cessation in pesticide intake leads to an insignificant decrease in ROS but does not reach the level of intact animals. An increase in the amount of 8-OHdG during subchronic intoxication with pesticides indicates the oxidative damage to DNA. In the oral fluid, the effect of thiram is accompanied by an increase in the amount of 8-OHdG on the day 21 of intoxication and in plasma at the day 7; at the same time, the level of 8-OHdG after the cessation of intoxication decreases to the control in the oral fluid and remains excessive in plasma. Imidacloprid leads to a growth in 8-OHdG in the oral fluid and plasma during 28 days of intoxication. After the cessation of the intake after 28 days, an increased content of 8-OHdG in the oral fluid and plasma remains. Imidacloprid causes a more pronounced oxidative effect on DNA as compared with thiram, which is accompanied by higher concentrations of 8‑OHdG in biological fluids. The accumulation of 8-OHdG indicates a depletion of antioxidant and reparative systems of the organism.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"19 1","pages":"30 - 36"},"PeriodicalIF":0.4,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145143602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cj0983 and Cj0090 Lipoproteins Contain Potential Linear Peptide Epitopes for Anti-Campylobacter jejuni Vaccine Applications","authors":"Jamai Pruline D. Sale,&nbsp;Monique M. Oro,&nbsp;Bennies T. Agnazata,&nbsp;Nedrick T. Distor","doi":"10.1134/S1990750824600821","DOIUrl":"10.1134/S1990750824600821","url":null,"abstract":"<p><i>Campylobacter jejuni</i>, a Gram-negative bacterium, is the major etiologic agent of campylobacteriosis responsible for approximately 90% of reported foodborne infections globally. As a result, alternative approaches such as bacterial vaccines may play pivotal roles in mitigating these bacterial public health concerns. Although there are ongoing studies on the development of vaccine candidates based on antigenic molecules of <i>C. jejuni</i>, the potential of Cj0983 and Cj0090 to be independently considered in the preparation of a protective lipoprotein antigen-based anti-<i>C. jejuni</i> vaccine remains elusive. Herein, the potential linear antigenic epitopes in Cj0983 and Cj0090 from <i>C. jejuni</i> were investigated for possible vaccine applications. Linear peptide epitopes were identified and evaluated for their antigenic and non-allergenic properties using VaxiJen and AllergenFP, respectively. Comprehensive bioinformatics-based in silico analyses revealed that there were 37 B-cell, 371 Class I T-cell, and 365 Class II T-cell peptide epitopes found in Cj0983. In comparison, there were 10 B-cell, 63 Class I T-cell, and 93 Class II T-cell peptide epitopes found in Cj0090. Through PyMOL, selected molecular interactions were screened and predicted between chains of human leukocyte antigen (HLA)-peptide epitope complexes, revealing that H-bonding is the predominant intermolecular force during binding. Our results identified the top three peptide epitopes for Cj0983 (³¹²QNDDYKLNLDLKFKN³²⁶, ³¹¹TQNDDYKLNLDLKFK³²⁵, and ³⁰⁹NITQNDDYKLNLDLKF³²⁴) and Cj0090 (⁹⁸QEVILRKLASDTRAND¹¹³, ¹⁰⁶ASDTRANDFRLEIKA¹²⁰, and ¹¹³DFRLEIKAK¹²¹) which have great potentials as vaccine components and can further be utilized as contributing protein-derived molecules towards advancing anti-<i>C. jejuni</i> vaccine development.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"19 1","pages":"109 - 122"},"PeriodicalIF":0.4,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145143236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermodynamic and Electrochemical Characteristics of Urine Protein Molecules That Affect the Formation of Stones","authors":"A. S. Tatevosyan,&nbsp;A. V. Bunyakin,&nbsp;S. N. Alekseenko,&nbsp;Z. O. Katani","doi":"10.1134/S1990750824601346","DOIUrl":"10.1134/S1990750824601346","url":null,"abstract":"&lt;p&gt;Pathogenetic development of urolithiasis (UL) can be divided chronologically into prestone phase and stone phase. Specifically, the stone phase (SP) occurs from the moment when Randall plaque (RP), which is calcium phosphate (CaP) in 100% of cases, having destroyed the epithelial layer of the papilla in the renal calyx, comes into direct contact with urine, which is saturated not only with numerous inorganic microelements but also with various organic molecules, which always leave “traces of protein.” The study of the composition of the stone shows that, in addition to inorganic substances, there is always a protein component, the role of which has not yet been fully disclosed. The most unclear question remains why urinary proteins (uromodulin, osteopontin…) are able to both inhibit stone growth and act as promoters of stone formation. No less intriguing is the question of why calcium oxalate (CaOx) is the most common calculus in the urinary system. Objective. To determine the features of thermodynamic (TD) and electrochemical (EC) mechanisms that promote the growth of urinary calculus on the calcium phosphate (CaP) template already formed in the renal papilla in the form of RP and to establish the role of the protein (organic) component in this process as well as to find out the reasons why CaOx is the most common calculus in the urinary system. Materials and methods. A metaanalysis of TD and EC mechanisms promoting the growth of urinary calculus on the already formed CaP “seed” in the form of RP was performed, and the effect of the isoelectric point (pI) of proteins deposited on this CaP template was determined. Considering that CaOx stones are the most common (60–80%), the рI of urinary protein molecules affecting their growth was compared with the features of circadian urine pH fluctuations. An analogy of the adhesive theory of electrical and electron formation of complex compounds that determine metal−ligand homeostasis is presented. Results and discussion. Proteins (peptides, amino acids) are weak electrochemical molecules, on the outer surface of which (depending on the pH of the external environment) a cationic or anionic charge prevails. An individual feature of any protein is its specific рI, in which the protein loses its EC activity and precipitates. Subsequently, the corresponding inorganic substances floating in the urine settle on the already aggregated protein layer. As a rule, рI of any protein is located within narrow limits of pH fluctuations not exceeding 1 unit (∆рН &lt; 1), while, for the aggregation of the protein, in addition to its stay in рI, time is needed, which determines the rate; i.e., a long stay of the protein in pI is necessary for the formation of an organic layer on the RP. The range of physiological fluctuations in urine pH during the day varies from 5.0 to 8.0 (∆pH ≈ 3). Obviously, with such a 1000-fold range of proton (H&lt;sup&gt;+&lt;/sup&gt;) oscillations, none of the proteins present in the urine have time to agg","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"19 1","pages":"68 - 79"},"PeriodicalIF":0.4,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145144000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organotin Complexes—Candidates for Antitumor Agents: Toxicity vs. Pharmaceutical Activity","authors":"M. A. Dodokhova,&nbsp;I. M. Kotieva,&nbsp;M. S. Alkhusein-Kulyaginova,&nbsp;V. M. Kotieva,&nbsp;E. M. Kotieva,&nbsp;D. A. Berseneva,&nbsp;D. B. Shpakovsky,&nbsp;N. S. Silin,&nbsp;M. V. Gulyan,&nbsp;E. R. Milaeva","doi":"10.1134/S1990750824600791","DOIUrl":"10.1134/S1990750824600791","url":null,"abstract":"<p>Chemotherapy is one of the promising areas for the treatment of cancer. Platinum-based drugs are widely used in clinical practice. However, due to their high cost and a number of limitations related to their toxicity and resistance to a number of tumors, other effective drugs based on metals, in particular organotin compounds and their complexes with various ligands, are under study. The review provides data on the toxicity of Sn(IV) compounds, their effect on the human body and also analyzes the pharmacotherapeutic potential at various levels both in vitro and in vivo in animals of chemically modified organotin candidates for therapeutic agents over the past 10 years. Special attention is paid to the study of the antiproliferative activity of organotins with protective, antioxidant, and steroid fragments. Antitumor and antimetastatic activity has been detected for a number of Sn(IV) complexes in experiments on a number of tumor models in vivo. The analysis of the obtained results sets the vector of development in conducting preclinical studies of such compounds from the selection of candidates, the search for animal models with tumor processes, methods of introduction into the body, etc. The proposed approach can be used to achieve a rational activity to toxicity ratio and reduce side effects during the course use of organotin complexes as chemotherapeutic agents.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"19 1","pages":"1 - 20"},"PeriodicalIF":0.4,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145143232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peculiar Properties of Lipid Composition of Erythrocyte Membranes and Their Physiological Parameters in the Indigenous and Newly Arrived Population of the North of Russia","authors":"N. F. Kushnerova,&nbsp;S. E. Fomenko,&nbsp;V. G. Sprygin,&nbsp;T. V. Momot","doi":"10.1134/S1990750825600050","DOIUrl":"10.1134/S1990750825600050","url":null,"abstract":"<p>A comparative analysis of the quantitative composition of phospholipids and fatty acids of blood erythrocyte membranes and their dimensional characteristics and hemolytic resistance to hemolysis in indigenous and nonindigenous populations of the North of the Russian Federation was carried out. The data on ethnic peculiarities of metabolic reactions in representatives of two ethnic groups (the indigenous population of Mongoloids and the nonindigenous population of Caucasians) were obtained. A notable alteration in the ratio of phospholipid fractions within the erythrocyte membrane was observed in the indigenous Chukchi population. This involved a decline in phosphatidylcholine and its replacement by sphingomyelin and an increase in the values of phosphatidylethanolamine and metabolically active fractions, including phosphatidylserine, phosphatidylinositol, and phosphatidic acid. These findings contrast with those observed in the newly arrived population. Additionally, notable discrepancies were observed in the fatty acid profiles of erythrocyte membrane lipids. The predominant presence of n-3 polyunsaturated fatty acids in the overall lipid composition and in the primary structural phospholipids, namely phosphatidylcholine (PC) and phosphatidylethanolamine (PE), substantiates the ethnic distinctions observed in both indigenous and immigrant populations. These differences in the composition of polyunsaturated fatty acids in the structures of PC and PE indicate the emergence of modified molecular species of these fractions in the indigenous population. The aforementioned ethnic peculiarities in the phospholipid component of erythrocyte membranes result in differences in the dimensional characteristics of erythrocytes and an increase in the resistance of cells to hemolysis in individuals of the indigenous population. In the authors’ opinion, this is not evidence of any deviation from the norm but is more likely to be associated with one of the variants of human metabolism.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"19 1","pages":"21 - 29"},"PeriodicalIF":0.4,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145143603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Sodium Selenite on Lipid Peroxidation Processes and Concentration of Sex Hormones during Intensive Physical Exercise","authors":"V. V. Kornyakova,&nbsp;E. A. Chigrinski,&nbsp;V. D. Konvay,&nbsp;P. P. Zolin","doi":"10.1134/S199075082460105X","DOIUrl":"10.1134/S199075082460105X","url":null,"abstract":"<p>The expression of selenoproteins, which in turn depends on the provision of tissues with exogenous selenium supplied with food, is important in the functioning of the antioxidant system. There is data on an increase in the intensity of free radical processes with a deficiency of selenium in the organism. The aim of this study is to establish the effect of sodium selenite on lipid peroxidation processes and concentration of sex hormones during intensive physical exercise. The study was carried out on 60 male rats that were divided into four groups (<i>n</i> = 15). The first group included the control animals. The second and third groups were experimental (animals of these groups swam with the same load (10% of the body weight) but differed in the intensity of training). Animals of the fourth group swam with the load of 10% and received sodium selenite (Na₂SeO₃) at a dose of 30 μg/day/kg of body weight. After completing the experiment, the blood was taken from animals to study biochemical indices (total testosterone, free testosterone, thyroxine, triiodothyronine, malondialdehyde, glutathione, activity of glutathione peroxidase and glutathione reductase). During the experiment, it was established that intensive physical exercise causes a decrease in concentration of the studied hormones in the blood serum and contribute to the intensification of free radical oxidation and processes of lipid peroxidation, which is evidenced by an increase in the content of malonic dialdehyde in erythrocytes and a change in the parameters of the glutathione antioxidant system. The administration of sodium selenite at a dose of 30 μg/day/kg of body weight contributes to the restoration of the level of sex steroids, without affecting the level of thyroid hormones and contributes to a decrease in the level of malonic dialdehyde and an increase in the concentration of glutathione as compared with the third group. The activity of glutathione-dependent enzymes under the effect of sodium selenite also increases. Thus, high intensity physical exercise contributes to an increase in the content of lipid peroxidation products in the blood, inhibition of antioxidant function, and a decrease in the level of thyroid and sex hormones. The administration of sodium selenite contributes to a decrease in free radical processes and an increase in the concentration of sex hormones but has no effect on the content of thyroid hormones as compared with the group of rats experiencing intensive physical exercise.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"19 1","pages":"37 - 40"},"PeriodicalIF":0.4,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145143237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of Metabolic Plasticity of the Brain in Animals with Modeled Parkinson’s Disease","authors":"A. K. Berdnikov,&nbsp;N. A. Rozanova,&nbsp;S. V. Novikova,&nbsp;N. A. Kolot’eva","doi":"10.1134/S1990750824601140","DOIUrl":"10.1134/S1990750824601140","url":null,"abstract":"<p>Neuroinflammation plays the key role in progression of Parkinson’s disease (PD), the neurodegenerative disorder characterized by the progressive loss of dopaminergic neurons of the substantia nigra, which results in the motor impairment. The major pathogenetic mechanisms of PD are metabolic dysfunctions, mitochondrial impairments and inflammatory response determined by the activation of microglia. Understanding the molecular pathways underlying these processes is an important stage in the development of target therapeutic strategies. Recent studies have emphasized the significance of lactate metabolism and the related signaling pathways in modulation of both neuroinflammation and energy homeostasis. GPR81, also known as HCAR1, is a lactate receptor involved in the regulation of metabolism and inflammatory processes. In spite of the well-studied role of this receptor in peripheral tissues, its involvement in the pathogenesis of neurodegenerative diseases such as PD yet remains insufficiently studied. In the present study, the expression of GPR81 in the substantia nigra of the rat brain is studied under the conditions of LPS-induced PD model with the accent on its potential role in the regulation of inflammatory and metabolic processes. The analysis of dynamic changes in the expression of GPR81 will reveal its contribution to neuroprotection and metabolic plasticity of the brain, opening up new prospects for slowing down neurodegeneration in PD.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"19 1","pages":"60 - 67"},"PeriodicalIF":0.4,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145143999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Replication-Competent Adenoviral Particles in the Vector Vaccines Salnavac® and Gam-Covid-Vac® against the New Coronavirus Infection Covid-19","authors":"D. Y. Kolomiytseva,&nbsp;N. A. Litvinova,&nbsp;R. R. Shukurov","doi":"10.1134/S1990750824600717","DOIUrl":"10.1134/S1990750824600717","url":null,"abstract":"<p>The 2019–2022 coronavirus pandemic has brought vaccination to the forefront in recent years. As a result, various approaches to vaccine development and their efficacy and safety assessment have emerged. Methods for assessing recombinant vector vaccines using the specific safety criteria (SSC) have been developed in accordance with the FDA requirements. This resulted in the discovery of a link between the ratio of viral particles to cell suspension density and the characteristics of plaques (shape, size, absence of confluent plaques). A vaccine sample was added to a suspension of trypsinized adherent cells. The optimal density for this analysis is 0.6 million/mL for a T-175 culture flask. The study found that Neutral Red was the best dye for intravital cell staining. It enabled the differentiation of dead cells from living cells via cytoplasmic inclusions. The study produced a method with a lower limit of quantification (LLOQ) of 3 PFU/dose that satisfies international regulatory requirements, including those of the Food and Drug Administration (FDA).</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"19 1","pages":"89 - 97"},"PeriodicalIF":0.4,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145143234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}