Agriculture-BaselPub Date : 2025-07-10DOI: 10.3390/agriculture15141481
Hongyan Gao, Yuanye Liu, Gen Li, Haodong Liu, Yuxi Shang, Zheng Ma
{"title":"On-Demand Design of Terahertz Metasurface Sensors for Detecting Plant Endogenous and Exogenous Molecules","authors":"Hongyan Gao, Yuanye Liu, Gen Li, Haodong Liu, Yuxi Shang, Zheng Ma","doi":"10.3390/agriculture15141481","DOIUrl":"https://doi.org/10.3390/agriculture15141481","url":null,"abstract":"This study presents a neural-network-based method for on-demand design of terahertz metasurface sensors, aimed at detecting plant endogenous and exogenous molecules. The approach uses target performance indicators (constructed via fingerprint peaks) as inputs and structural parameters as outputs, employing a neural network to map the complex relationship between them. Two single-resonant-peak metasurface sensors were developed to detect abscisic acid and gibberellic acid. The abscisic acid metasurface sensor achieved an average MSE of 5.66 × 10−6 and RER of 0.167%, while the gibberellic acid metasurface sensor had an average MSE of 8 × 10−7 and RER of 0.086%. Their resonant peaks highly matched the substance fingerprint peaks, enabling specific detection. Metasurface sensors’ sensitivities were effectively controlled using correlation analysis and neural networks, achieving remarkable levels of 156.7 and 150.1 GHz/RIU, allowing trace detection. Three dual-resonant-peak metasurface sensors were designed to improve the detection specificity for chlorophyll and folpet and to detect chlorophyll and folpet simultaneously. These metasurface sensors exhibited average MSEs of 1.4 × 10−5, 1.6 × 10−6, 1.35 × 10−5 and RERs of 0.27%, 0.088%, 0.20%. The model also worked for four other plant-related molecules, proving its strong generalization ability. Overall, for different application scenarios of exogenous and endogenous molecules in plants, the on-demand design methodology offers a whole new set of ideas for quickly designing and widely applying metasurface sensors with suitable performance indicators.","PeriodicalId":48587,"journal":{"name":"Agriculture-Basel","volume":"15 14","pages":"1481-1481"},"PeriodicalIF":0.0,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2077-0472/15/14/1481/pdf?version=1752151644","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Agriculture-BaselPub Date : 2025-06-30DOI: 10.3390/agriculture15131407
Yuan Qi, Juan Qi, Wenhui Liu, Wenhua Du, Mingjie Li
{"title":"Effects of Exogenous Plant Hormones on Agronomic Traits and Physiological Responses of Elymus sibiricus","authors":"Yuan Qi, Juan Qi, Wenhui Liu, Wenhua Du, Mingjie Li","doi":"10.3390/agriculture15131407","DOIUrl":"https://doi.org/10.3390/agriculture15131407","url":null,"abstract":"As key endogenous signal molecules regulating plant growth processes, plant hormones have significant applications in forage breeding. The experiment used ‘Elymus sibiricus Qingmu No. 2’ as the test material, and the effects of foliar applied phytohormones of gibberellin (GA3: 50, 100, 200, and 300 mg/L), cytokinin (6-BA: 1, 10, 100, and 150 mg/L), epibrassinolide (EBR: 0.01, 0.1, 1, and 10 mg/L), zeatin (ZT: 1, 10, 20, and 100 mg/L), and auxin (IAA: 10, 50, 100, and 150 mg/L) on growth and physiological responses in Elymus sibiricus were investigated. The results indicated that GA3 at 200 mg/L significantly enhanced biomass by 38.19%, plant height by 75.11%, and leaf area by 40.58% compared to controls. IAA (150 mg/L) specifically increased stem diameter by 38.25%, while 6-BA (100 mg/L) elevated chlorophyll content and antioxidant enzyme activities, indicating dual photoprotective and stress-mitigating roles. EBR (1 mg/L) and ZT (20 mg/L) moderately enhanced growth metrics. All treatments universally boosted stress tolerance via soluble sugar/protein accumulation and antioxidant system activation. Through comprehensive analysis, we recommend GA3 (200 mg/L) for effective grassland improvement, propose synergistic combinations of 6-BA and IAA to overcome morphological limitations, and highlight ultra-low EBR (0.01–0.1 mg/L) as a priority for future research.","PeriodicalId":48587,"journal":{"name":"Agriculture-Basel","volume":"15 13","pages":"1407-1407"},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2077-0472/15/13/1407/pdf?version=1751273798","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Optimization of Phenolic Compound Extraction from Pineapple Leaf Fiber: Stability Enhancement and Application in Mango Preservation","authors":"Yijun Liu, Huifang Ma, Yuhan Wang, Qiuyu Li, Minyong Shi, Gang Chen","doi":"10.3390/agriculture15111203","DOIUrl":"https://doi.org/10.3390/agriculture15111203","url":null,"abstract":"Focusing on the challenges of low utilization of pineapple leaves and the poor stability of phenolic compounds (PCs) in pineapple leaf fiber using traditional solvent extraction methods, the effects of different extraction media (including distilled water, neutral methanol, acidic methanol, alkaline methanol, neutral ethanol, and alkaline ethanol) was systematically investigated on the extraction efficiency of PCs from pineapple leaf fiber. Through response surface methodology (RSM), the optimal extraction conditions were determined. Additionally, the impacts of illumination and pH on the stability of PCs in pineapple leaf fiber were thoroughly examined. The results demonstrated that acidic ethanol outperformed other extraction media, with the optimized extraction conditions as follows: ethanol concentration = 80%, material-to-liquid ratio = 1:40, extraction temperature = 70 °C, and extraction time = 40 min. Under these conditions, the extraction yield of PCs reached 11.55 mg/g. Furthermore, the stability of PCs was significantly enhanced by minimizing light exposure and maintaining a pH range of 3–6. The potential application of PCs extracted from pineapple leaf fiber was also explored, particularly in mango preservation, revealing promising results. This study not only provides an efficient and sustainable approach for the extraction and stabilization of PCs from pineapple leaf fiber but also opens new avenues for the utilization of polyphenols in functional applications, contributing to the valorization of agricultural by-products.","PeriodicalId":48587,"journal":{"name":"Agriculture-Basel","volume":"15 11","pages":"1203-1203"},"PeriodicalIF":0.0,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2077-0472/15/11/1203/pdf?version=1748683866","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147880259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Agriculture-BaselPub Date : 2025-05-29DOI: 10.3390/agriculture15111177
Xue Tao, Yi Li, Zimo Chen, Chao Kong, Biyun Feng, Changyin Zhu, Yinlong Zhang, Jianming Xue, Hu Cheng
{"title":"Converting Agroforestry Biowaste into Stable Near-Natural Chars via Hydrothermal Humification and Pyrolysis for Immobilizing Plasticizer","authors":"Xue Tao, Yi Li, Zimo Chen, Chao Kong, Biyun Feng, Changyin Zhu, Yinlong Zhang, Jianming Xue, Hu Cheng","doi":"10.3390/agriculture15111177","DOIUrl":"https://doi.org/10.3390/agriculture15111177","url":null,"abstract":"To ensure agricultural safety and ecological security, it is crucial to effectively immobilize emerging organic pollutants, such as plasticizers, to prevent their migration in various environmental matrices. However, the ideal immobilization agent with the advantages of being environmentally friendly is very rare. In this study, low-cost and stable near-natural immobilization agents, char-derived artificial humic acids, CHAs, were proposed and prepared via hydrothermal humification (180 °C) and pyrolysis (300, 500, or 700 °C) of agroforestry biowaste. The resulting CHAs exhibit high purity (composed primarily of C (67.28–81.35%), O (6.65–21.64%), H (1.40–5.28%), and N (0.36–0.58%)) with remarkably low ash content (5.43–10.02%). Characterization revealed a compact structure with a limited porosity with small surface area (0.27–0.32 m2 g−1) and pore volume (2.99–3.43 × 10−4 cm3 g−1). Notably, high-temperature pyrolysis induced consumption of oxygen-containing functional groups while promoting aromatic structure formation. The sorption behavior of diethyl phthalate, a representative plasticizer, on CHAs was well described by both Langmuir isotherm and pseudo-second-order kinetic models. The CHAs exhibited remarkable sorption performance for diethyl phthalate, with a maximum sorption capacity reaching 3345 mg kg−1 as determined by the Langmuir model. The sorption of diethyl phthalate onto CHAs is mainly multi-layer sorption dominated by physical processes, mainly including pore filling, partitioning, hydrogen bonding, and π–π stacking. Mean sorption energies ranging from 2.56 to 4.99 × 10−3 kJ mol−1 indicate the predominance of physical sorption mechanisms. This study developed a method to convert the liquid by-product produced during hydrothermal humification of biowaste into stable near-natural and carbon-rich char materials, and the proposed materials show great promise in immobilizing pollutants from various environmental matrices.","PeriodicalId":48587,"journal":{"name":"Agriculture-Basel","volume":"15 11","pages":"1177-1177"},"PeriodicalIF":0.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2077-0472/15/11/1177/pdf?version=1748527083","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147895781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Agriculture-BaselPub Date : 2025-05-23DOI: 10.3390/agriculture15111128
Yuqing Jing, Jian Yang, Renyi Ma, Bo Lan, Siyang Li, Qian Zhang, Fang Du, Qianqian Guo, Kangquan Yin
{"title":"DNA Polymerase Theta Regulates the Growth and Development of Fusarium acuminatum and Its Virulence on Alfalfa","authors":"Yuqing Jing, Jian Yang, Renyi Ma, Bo Lan, Siyang Li, Qian Zhang, Fang Du, Qianqian Guo, Kangquan Yin","doi":"10.3390/agriculture15111128","DOIUrl":"https://doi.org/10.3390/agriculture15111128","url":null,"abstract":"Fusarium acuminatum is a major pathogenic fungus causing root rot in alfalfa (Medicago sativa). DNA polymerase theta is known to play a crucial role in repairing DNA double-strand breaks. However, its biological function in F. acuminatum remains unknown. In this study, the POLQ gene was deleted by homologous recombination using Agrobacterium tumefaciens-mediated transformation. Compared to the wild type (with the POLQ gene), the mutants (without the POLQ gene) showed significant phenotypic changes: they produced brown-yellow pigments instead of pink, slowed mycelial growth, and exhibited changes in macroconidia size and shape. The virulence of the mutants was greatly reduced, inducing only mild symptoms in alfalfa. In addition, FITC-WGA staining showed impaired spore germination and hyphal growth. These results suggest that POLQ is a key gene regulating growth and development of F. acuminatum, indicating that DNA repair may play an essential role in the pathogenicity of the pathogen in alfalfa. The POLQ gene could thus be a promising target for limiting F. acuminatum infections in alfalfa.","PeriodicalId":48587,"journal":{"name":"Agriculture-Basel","volume":"15 11","pages":"1128-1128"},"PeriodicalIF":0.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2077-0472/15/11/1128/pdf?version=1748010004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147896737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Agriculture-BaselPub Date : 2025-05-16DOI: 10.3390/agriculture15101078
Said Mohammed, Klaus Mummenhoff
{"title":"Germination Under Temperature Stress Facilitates Invasion in Indehiscent Lepidium Species","authors":"Said Mohammed, Klaus Mummenhoff","doi":"10.3390/agriculture15101078","DOIUrl":"https://doi.org/10.3390/agriculture15101078","url":null,"abstract":"This study investigates the germination ecology of three Lepidium species, including the invasive, indehiscent-fruited Lepidium appelianum and Lepidium draba, and the invasive, dehiscent-fruited Lepidium campestre. The ability of Lepidium species to germinate under a wide range of temperature conditions is significant for understanding their potential invasiveness and establishment in novel and extreme environments. This study aims to clarify the germination behavior of L. appelianum, L. draba, and L. campestre, thereby enhancing our understanding of their invasive potential and ecological implications in the context of a changing climate. The base (Tb), optimum (To), and maximum temperatures for 50% germination (Tc(50)) were determined across a broad thermal gradient following standard protocols. Freshly harvested seeds and fruits of L. appelianum are non-dormant. In contrast, L. draba exhibit pericarp-mediated chemical dormancy, while L. campestre demonstrates physiological dormancy, which is released through after-ripening. The results indicate that L. appelianum and L. draba seeds and fruits germinate at a base temperature (Tb) of 1 °C and 4 °C, respectively. On the other hand, L. campestre seeds germinate at a Tb of 5.8 °C. The optimum temperature (To) for the germination of seeds and fruits in L. appelianum and L. draba ranges from 23 °C to 25 °C, while the To for L. campestre seed germination is 16 °C to 18 °C. Additionally, the maximum temperature for 50% germination (Tc(50)) for L. appelianum fruits is 39.8 °C, for L. draba it is 34.4 °C, and L. campestre reports a (Tc(50)) ranging from 27.4 °C to 33.3 °C for freshly harvested and after-ripened seeds, respectively. These results demonstrated that L. appelianum and L. draba can germinate across a broad temperature range, from very cold to very hot, unlike L. campestre. These findings suggest that the unique reproductive strategy of indehiscent fruits, coupled with a wide thermal germination niche, may contribute to the invasive success of L. appelianum and L. draba. Given the projected climate warming, the results highlight the potential for increased invasiveness of these species and suggest the need for targeted management strategies.","PeriodicalId":48587,"journal":{"name":"Agriculture-Basel","volume":"15 10","pages":"1078-1078"},"PeriodicalIF":0.0,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2077-0472/15/10/1078/pdf?version=1747640068","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147896790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Agriculture-BaselPub Date : 2025-05-02DOI: 10.3390/agriculture15090990
Anna Martikainen
{"title":"Assessing the Resilience of Farming Systems: Insights from the Common Agricultural Policy and Polish Fruit and Vegetable Farming Challenges","authors":"Anna Martikainen","doi":"10.3390/agriculture15090990","DOIUrl":"https://doi.org/10.3390/agriculture15090990","url":null,"abstract":"Risk management and resilience of agriculture are among the most important issues in the ongoing discussion on the shape of the Common Agricultural Policy (CAP). Farming systems face various risks that increase their vulnerability, which necessitates the strengthening of their resilience. This raises critical questions whether CAP policies adequately support the resilience of farming systems in addressing these challenges. The study investigates the resilience of the Polish fruit and vegetable farming system within the context of the CAP. Employing a mixed-methods approach that includes interviews and stakeholder workshops, the research identifies critical risks such as market volatility, climate change, labor shortages, or international competition. The study reveals that while farmers adopt various coping strategies, existing CAP measures predominantly support robustness, often neglecting adaptability and transformability, which are essential for addressing long-term risks. Stakeholder feedback highlights bureaucratic inefficiencies, limited access to resources for innovation, and an overemphasis on short-term interventions. Recommendations emphasize the need for policy adjustments to foster long-term adaptability through enhanced vertical and horizontal integration, support for innovation, and knowledge transfer. Under future scenarios, policy priorities vary but consistently call for resilience-focused reforms. These findings underscore the benefits of integrating resilience-thinking frameworks into agricultural policy to enable sustainable development and competitiveness of farming systems.","PeriodicalId":48587,"journal":{"name":"Agriculture-Basel","volume":"15 9","pages":"990-990"},"PeriodicalIF":0.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2077-0472/15/9/990/pdf?version=1746192721","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147910413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Agriculture-BaselPub Date : 2025-05-01Epub Date: 2025-05-08DOI: 10.3390/agriculture15101017
Yongping Yuan, Xiuying Wang, Verel Benson, Limei Ran
{"title":"An Integrated Multi-Media Modeling System for Regional- to National-Scale Nitrogen and Crop Productivity Assessments.","authors":"Yongping Yuan, Xiuying Wang, Verel Benson, Limei Ran","doi":"10.3390/agriculture15101017","DOIUrl":"10.3390/agriculture15101017","url":null,"abstract":"<p><p>Excessive nutrients transported from agricultural fields into the environment are causing environmental and ecological problems. This study uses an integrated multi-media modeling system version 1 (IMMMS 1.0) linking air, land surface, and watershed processes to assess corn grain yield and nitrogen (N) losses resulting from changing fertilization conditions across the contiguous United States. Two fertilizer management scenarios (FMSs) were compared and evaluated: 2006 FMS, developed based on the 2006 fertilizer sales data; and 2011 FMS, developed based on 2011 fertilizer sales and manure. Corn grain yields captured historical reported values with average percent errors of 4.8% and 0.7% for the 2006 FMS and 2011 FMS, respectively. Increased nitrogen (N) application of 21.2% resulted in a slightly increased corn grain yield of 5% in the 2011 FMS, but the simulated total N loss (through denitrification, volatilization, water, and sediment) increased to 49.3%. A better correlation was identified between crop N uptake and N application in the 2006 FMS (R<sup>2</sup> = 0.60) than the 2011 FMS (R<sup>2</sup> = 0.51), indicating that applied N was better utilized by crops in the 2006 FMS. Animal manure could create nutrient surpluses and lead to greater N loss, as identified in the regions of the Pacific and Southern Plains in the 2011 FMS. Manure nutrient management is important and urgently needed to protect our air and water quality. The IMMMS 1.0 is responsive to different FMSs and can be utilized to address alternative management scenarios to determine their impact when addressing the sustainability of food production and environmental issues.</p>","PeriodicalId":48587,"journal":{"name":"Agriculture-Basel","volume":"15 10","pages":"1017"},"PeriodicalIF":3.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12338305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144822883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Agriculture-BaselPub Date : 2025-04-24DOI: 10.3390/agriculture15090930
Mengmeng Xu, Le Liu, Wenjing Duan, Lizhu Niu, He Cheng, Chenyang Du, Mengyun Li, Wenying Huo, Hongyu Deng, Pan Zhou, Wen Chen, Long Che
{"title":"Daidzein Promotes the Proliferation of Porcine Mammary Epithelial Cells Through the mTOR Signaling Pathway","authors":"Mengmeng Xu, Le Liu, Wenjing Duan, Lizhu Niu, He Cheng, Chenyang Du, Mengyun Li, Wenying Huo, Hongyu Deng, Pan Zhou, Wen Chen, Long Che","doi":"10.3390/agriculture15090930","DOIUrl":"https://doi.org/10.3390/agriculture15090930","url":null,"abstract":"The purpose of this study was to examine the effect of daidzein on the proliferation of porcine mammary epithelial cells (PMECs) and elucidate the underlying molecular mechanisms. PMECs were treated with varying daidzein or rapamycin levels, and then cell proliferation and mTOR pathway protein expression were detected. When the concentration of daidzein added was in the range of 0–80 μM, cell proliferation was significantly promoted (p < 0.05). These results were in agreement with those obtained using the 5-ethynyl-2′-deoxyuridine (EdU) assay. Daidzein administration at 20 and 40 μM concentrations triggered significant activation of the mTOR signaling cascade and enhanced expression of downstream cell-cycle-regulatory proteins (cyclin D1) (p < 0.05). Moreover, exposure to 40 μM daidzein attenuated apoptotic signaling, as evidenced by reduced levels of Bax protein and cleaved caspase-3 (p < 0.05). These effects were reversed when rapamycin was used to inhibit the mTOR pathway. In conclusion, our findings suggest that daidzein activates PMEC proliferation via the mTOR pathway. The present work not only characterizes new functional properties of daidzein but also establishes mechanistic evidence supporting its role in augmenting sow lactation efficiency.","PeriodicalId":48587,"journal":{"name":"Agriculture-Basel","volume":"15 9","pages":"930-930"},"PeriodicalIF":0.0,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2077-0472/15/9/930/pdf?version=1745491726","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147905603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Long Non-Coding RNA lncXIRP1 Regulates the Proliferation and Apoptosis of Pig Leydig Cells","authors":"Haiyan Yang, Xiu‐Zhang Lu, Shan Zhang, Qi Tang, Xianyong Lan, Jing Wang, Xiaolei Chen, Chuanying Pan","doi":"10.3390/agriculture15080802","DOIUrl":"https://doi.org/10.3390/agriculture15080802","url":null,"abstract":"Leydig cells (LCs) originate from stem Leydig cells (SLCs) and synthesize testosterone, a hormone indispensable for the development, sustenance, and functionality of the male reproductive system. Accumulating evidence suggests that long non-coding RNAs (lncRNAs) play pivotal roles in animal reproductive processes, yet the functional contributions of lncRNAs in pig LCs remain largely uncharacterized. The aim of this study was to examine how lncRNAs influence the function of LCs and their underlying molecular regulatory mechanisms. To achieve this, RNA-seq was conducted on cells before ethane dimethane sulfonate (EDS) treatment (SLCs and LCs) and after EDS treatment (SLCs), identifying 887 significantly downregulated lncRNAs and 30 upregulated lncRNAs after EDS treatment. Bioinformatics analysis identified lncXIRP1 for further investigation. The effects of lncXIRP1 on LCs proliferation, apoptosis, and expression of genes related to testosterone synthesis were investigated by using RT-qPCR, Western blot, CCK-8 and other methods. Bioinformatics predictions have unveiled the existence of a binding site between lncXIRP1 and IGFBP3. Through RT-qPCR experiments and a dual-luciferase reporter system, it was conclusively demonstrated that lncXIRP1 has the capacity to repress the expression of IGFBP3 mRNA, thereby inhibiting the proliferation and transcription activity of genes associated with testosterone synthesis in LCs and promoting their apoptosis. These results provide a theoretical foundation for further exploration of the impact of lncRNAs on LCs function and improving pig reproductive performance.","PeriodicalId":48587,"journal":{"name":"Agriculture-Basel","volume":"15 8","pages":"802-802"},"PeriodicalIF":0.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2077-0472/15/8/802/pdf?version=1744107037","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147915655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}