Agriculture-Basel最新文献

{"title":"Combined Application of Magnesium Fertilizer and Biochar Boosts Tea (Camellia sinensis) Quality Through Soil Quality and Photosynthetic Traits Improvement","authors":"Xinyue Zhang, Xianyu Cheng, Diao Yan, Hao Guo, Zhigang Yi","doi":"10.3390/agriculture16151630","DOIUrl":"https://doi.org/10.3390/agriculture16151630","url":null,"abstract":"The application of biochar and magnesium (Mg) fertilizer can alleviate acidification, improve soil fertility, and enhance tea quality. However, the effects of different biochar application rates on soil properties, tea photosynthesis, and quality remain unclear. To elucidate their effects, a 90-day pot experiment was conducted with nine treatments: conventional fertilization (CK), improved conventional fertilization (CF), Mg fertilizer (MF), low/medium/high biochar (LBCF, MBCF, HBCF), and their combination with Mg (LBMF, MBMF, HBMF) in a controlled growth chamber, and soil properties, tea plant photosynthesis, and tea quality were measured. The results showed that higher application rates of biochar with Mg significantly alleviated soil acidification, increasing pH by 0.20–0.24 units. HBMF significantly enhanced key soil nutrients: total carbon (+198.11%), total nitrogen (+116.43%), and ammonium nitrogen (+73.89%). Ex-Mg increased with Mg addition and was further promoted by biochar, and photosynthetic rates increased by 71.69–101.41% in biochar and Mg application. These improvements increased free amino acids, caffeine, and soluble sugars in tea leaves by 39.56%, 46.88%, and 30.79% respectively under HBMF. Additionally, the relative contents of alcohols and esters increased by 93.29% and 62.20%, with marked rises in linalool and methyl salicylate. The increased tea quality is linked to higher activities of β-glucosidase and lipoxygenase observed in HBMF. In conclusion, the combined application of high biochar (2% of soil weight) and Mg fertilizer (15% of fertilizer weight) effectively improved soil quality, enhanced photosynthesis, and ultimately elevated both the taste and aroma quality of tea.","PeriodicalId":48587,"journal":{"name":"Agriculture-Basel","volume":"16 15","pages":"1630-1630"},"PeriodicalIF":0.0,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148861315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal Efficacy and Mechanisms of Methyleugenol from Asarum Essential Oil Against Rhizoctonia solani Causing Ginseng Damping-Off Disease","authors":"Rui Zhang, Xiaolin Chen, Xueqian Yan, Zhongliang Yang, Dandan Zhang, Yingping Wang, Baohui Lu","doi":"10.3390/agriculture16141547","DOIUrl":"https://doi.org/10.3390/agriculture16141547","url":null,"abstract":"Damping-off disease caused by Rhizoctonia solani is a devastating soilborne disease that poses a serious threat to sustainable ginseng (Panax ginseng) production. In an effort to develop environmentally friendly control alternatives, we investigated the volatile constituents of Asarum heterotropoides essential oil using GC-MS in 2023 and identified methyleugenol as the predominant bioactive component, accounting for 21.32% of the total volatile fraction. Mycelial growth rate assays demonstrated that methyleugenol exerted pronounced antifungal activity against R. solani, with EC50 and EC90 values of 60 mg/L and 800 mg/L, respectively. Treatment at 0.1 mg/mL resulted in 51% growth inhibition after 4 days of incubation. To elucidate the underlying mechanisms of action, R. solani mycelia exposed to 0.1 mg/mL methyleugenol were harvested at 4 and 6 days post-treatment for integrated transcriptomic and untargeted metabolomic profiling. Transcriptome analysis revealed 1444 and 1157 significantly downregulated genes at 4 and 6 days, respectively, with 836 genes consistently repressed at both time points. GO and KEGG enrichment analyses indicated that these persistently suppressed genes were predominantly associated with protein processing in the endoplasmic reticulum, protein export, tyrosine metabolism, and peroxisome biogenesis. Metabolomic profiling identified 95 differentially accumulated metabolites that were commonly reduced across both time points, with KEGG pathway enrichment highlighting significant suppression of starch and sucrose metabolism and galactose metabolism pathways central to carbon source utilization. Integrative transcriptome–metabolome correlation analysis further uncovered three key transcription factors (gene-RDB_LOCUS74145, gene-RDB_LOCUS26344, and gene-RDB_LOCUS52181) implicated in the regulation of carbohydrate metabolism and tricarboxylic acid cycle-related metabolite production. Collectively, our findings indicate that methyleugenol suppresses R. solani growth through a multi-target mechanism involving impairment of carbon source metabolism, disruption of protein processing and secretion, and attenuation of cellular energy supply. These results provide a mechanistic foundation for the development of botanical fungicide-based strategies for the green management of ginseng damping-off disease.","PeriodicalId":48587,"journal":{"name":"Agriculture-Basel","volume":"16 14","pages":"1547-1547"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2077-0472/16/14/1547/pdf?version=1784537624","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148672235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cloning, Expression, and Binding Characteristics of Chemosensory Protein 8 in Hippodamia variegata to Aphid-Induced Plant Volatiles","authors":"Benjie Zhang, Dongsheng Hu, Deqin Hu, Lingjia Bing, Yongsheng Yao, Hongsheng Pan, Weifeng Guo","doi":"10.3390/agriculture16141542","DOIUrl":"https://doi.org/10.3390/agriculture16141542","url":null,"abstract":"Hippodamia variegata is a key natural enemy of various aphid pests in agricultural ecosystems, and its foraging and oviposition behaviors are strongly dependent on chemical signals from prey aphids and host plants. In this study, we functionally characterized the chemosensory protein HvarCSP8 to elucidate its potential role in mediating olfactory responses to aphid-induced plant volatiles. The expression profiles of HvarCSP8 were examined by quantitative real-time PCR (qRT-PCR) across developmental stages and sexes; the recombinant HvarCSP8 was purified; the potential ligands specificity and affinity were studied using fluorescence-based competitive binding assays; and structural characteristics were obtained via homology modeling and molecular docking simulations. The results demonstrated that HvarCSP8 exhibits pronounced sex- and tissue-specific expression, with significant upregulation in adult antennae, particularly in females. The purified protein exhibited selective binding affinity to multiple aphid-induced plant volatiles, such as α-Farnesene, α-Terpinene, and 2-Ethyl-1-hexanol. Molecular docking revealed that HvarCSP8 interacts with these ligands through multiple amino acid binding sites, among which hydrophobic residues such as VAL29, LEU61, ILE64, and LEU65 frequently occur in binding sites for multiple ligands, suggesting that these residues play important roles in binding. These findings indicate that HvarCSP8 may be involved in the olfactory detection of aphid-induced plant volatiles in H. variegata, providing a candidate target for the development of behavior-based biological control strategies against aphid pests.","PeriodicalId":48587,"journal":{"name":"Agriculture-Basel","volume":"16 14","pages":"1542-1542"},"PeriodicalIF":0.0,"publicationDate":"2026-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148861314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Melatonin Supplementation on the Quality, Bacterial Community, and In Vitro Rumen Fermentation of Whole-Plant Soybean Silage","authors":"Donghui Hou, Meng He, X Li, Sui Wang, Xiaohong Tong, Yanqi Ma, Yuanhao Sun, Zheqi Bai, Yi Jiang","doi":"10.3390/agriculture16131467","DOIUrl":"https://doi.org/10.3390/agriculture16131467","url":null,"abstract":"Whole-plant soybean (WPS) is a high-protein forage resource, but its natural ensiling is often unsatisfactory due to low water-soluble carbohydrate content and high buffering capacity. This study investigated the effects of exogenous melatonin (ME) at 0 (CK), 5 (ME1), 10 (ME2), and 20 (ME3) mg/kg fresh matter on fermentation quality, chemical composition, in vitro rumen fermentation, and bacterial community structure of WPS silage. ME2 and ME3 had lower pH values and higher lactic acid contents than CK, with both treatments achieving pH values below 4.2. Crude protein concentration increased from 15.42% in CK to 19.96% in ME3, while neutral detergent fiber was lower in all ME treatments, and acid detergent fiber was lower in ME2 and ME3 than in CK. At 36 h, no overall treatment effect was detected for cumulative gas production, whereas in vitro dry matter digestibility differed only between ME2 and ME3. 16S rRNA gene sequencing revealed that ME altered the bacterial community, with community-weighted rrn copy number elevated in ME2 and ME3. Random forest analysis identified Enterococcus as the genus with the highest importance for treatment classification, and functional predictions indicated higher predicted abundances of amino acid biosynthesis pathways in ME treatment groups. These results indicate that ME has potential as an additive for improving WPS silage fermentation, but practical dosage recommendations require further validation through aerobic stability, animal performance, economic, and safety assessments.","PeriodicalId":48587,"journal":{"name":"Agriculture-Basel","volume":"16 13","pages":"1467-1467"},"PeriodicalIF":0.0,"publicationDate":"2026-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148672234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic Fermentation of Pu-Erh Tea Residue with a Tailored Bacterium and Enzymes: Unlocking Its Potential as a Sustainable Feed Ingredient","authors":"Ding Xie, Zicheng Zhang, Yongliang Wang, Luya Feng, Zhiyi Tang, Meijuan Bai, Xiaokang Ma, Bie Tan, Chen Zhang, Dingfu Xiao","doi":"10.3390/agriculture16101036","DOIUrl":"https://doi.org/10.3390/agriculture16101036","url":null,"abstract":"Pu-erh tea residue (Ptr), an insoluble byproduct generated during the industrial production of ready-to-drink Pu-erh beverages, is often discarded as waste despite its substantial nutritional potential. To enhance its feed value, this study developed a microbial–enzymatic synergistic fermentation system. A dominant strain, isolated from decaying tea residue, was identified as Bacillus albus and designated as Bacillus albus C01 (Bacillus.a C01). The Bacillus.a C01 strain was sensitive to all nine antibiotics tested and reduced system pH to 3.9 after 5 days of fermentation. 16S rRNA sequencing revealed that microbial–enzymatic synergistic fermentation enriched Bacillus spp., inhibited Pseudomonas spp., and optimized Ptr quality by upgrading nutrients and degrading tannins. Synergistic fermentation significantly increased the contents of crude protein (+7.50%), soluble protein (+93.32%), acid-soluble protein (+41.87%), reducing sugars (+506.49%), γ-aminobutyric acid (+28.00%), tea polyphenols (+7.47%), acetic acid (+22.87%), and total antioxidant capacity (+23.78%), while decreasing crude fiber (−26.61%), tannins (−90.10%), and suppressing polyphenol oxidase activity (−55.41%). In summary, co-fermentation of Ptr with Bacillus.a C01 and composite enzymes significantly improved its nutritional profile by increasing available proteins and organic acids, and enhancing antioxidant capacity, thereby upgrading this industrial byproduct into a functional feed ingredient. This approach offers a practical strategy to mitigate feed shortages and upcycle solid waste from the beverage industry.","PeriodicalId":48587,"journal":{"name":"Agriculture-Basel","volume":"16 10","pages":"1036-1036"},"PeriodicalIF":0.0,"publicationDate":"2026-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2077-0472/16/10/1036/pdf?version=1778410050","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147967050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zinc Oxide Nanoparticles Enhance Vigor of Aged Naked Oat Seeds: Transcriptomic Insights into Antioxidant and Metabolic Reprogramming","authors":"F. Chen, Yuan Ma, Kuiju Niu, Fangyuan Zhao, Yajiao Zhao, Ruirui Yao, Tao Shao, Huan Liu","doi":"10.3390/agriculture16080842","DOIUrl":"https://doi.org/10.3390/agriculture16080842","url":null,"abstract":"Naked oat (Avena nuda L.) is an important dual-purpose crop for grain and forage in cold regions; however, its high fatty acid content renders seeds prone to deterioration during storage. This study aimed to investigate the protective effects of zinc oxide nanoparticles (ZnO NPs) on artificially aged naked oat seeds and elucidate the underlying molecular mechanisms. Non-aged seeds (Naged) were subjected to artificial aging at 45 °C and 100% relative humidity for 24 h (Aged), followed by priming with 30 mg L−1 ZnO NPs for 6 h (Daged). Antioxidant enzyme activities were determined spectrophotometrically, and transcriptome sequencing was performed on an Illumina platform to identify differentially expressed genes (DEGs) and enriched pathways. We found that ZnO NPs increased catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD) activities by 3–4-fold, restored germination rate from 75% to 98%, and enhanced seed vigor index. A total of 21,403 DEGs were detected, with 15,841 stably expressed in response to nano-priming. Reactive oxygen species (ROS) burst rapidly induced up-regulation of AP2/EREBP transcription factor family members, which subsequently activated antioxidant enzyme genes to maintain cellular redox homeostasis. Metabolic pathway analysis demonstrated that the phenylpropanoid pathway was reprogrammed, characterized by down-regulated lignin biosynthesis and up-regulated flavonoid production, thereby enhancing ROS scavenging capacity. Additionally, the pentose phosphate pathway was activated to provide additional NADPH for antioxidant defense, and up-regulated ADP-glucose pyrophosphorylase (AGPase) facilitated starch accumulation. Notably, the 40S ribosomal protein S13 exhibited the highest connectivity in protein–protein interaction networks, was up-regulated 2.1-fold, and was enriched in post-translational modification processes. These findings suggest that nano-priming with ZnO NPs represents a promising biotechnological strategy for enhancing seed vigor and storability in naked oat, with potential applications in sustainable agriculture and the seed industry.","PeriodicalId":48587,"journal":{"name":"Agriculture-Basel","volume":"16 8","pages":"842-842"},"PeriodicalIF":0.0,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2077-0472/16/8/842/pdf?version=1775819336","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Microplastics on the Environmental Fate of Atrazine in Soil: Adsorption and Dissipation","authors":"Zhao Cheng, Wenfeng Yang, Xinyun Zhou, Jian Chen, Hui Zhang, Kang Lv, Liya Ma, Jing Ge, Yaoyao Cao, Fidèle Suanon, Xiangyang Yu, Hongjie Sheng","doi":"10.3390/agriculture16060705","DOIUrl":"https://doi.org/10.3390/agriculture16060705","url":null,"abstract":"The accumulation of microplastics (MPs) in agricultural soils and atrazine in agricultural soils creates compound pollution that severely threatens soil health. The present study aimed to evaluate the effect of polyethylene (PE), polyvinyl chloride (PVC), and polybutylene succinate (PBS) on the adsorption and degradation of atrazine in yellow-brown and black soil. Batch adsorption kinetic and isotherm experiments were conducted in two distinct soils amended with MPs. A 90-day degradation experiment was performed to monitor atrazine persistence and the activities of key soil enzymes. The adsorption process was best described by the pseudo-second-order model and the Freundlich isotherm model, suggesting dominant chemisorption and multilayer adsorption on heterogeneous surfaces of the soil–MP composites. All MPs significantly enhanced the adsorption capacity for atrazine (6.80–39.93 mg kg−1), with the order PBS > PE > PVC. Furthermore, the degradation of atrazine was impeded by all MPs, with PVC exhibiting the strongest inhibitory effect. The half-life of atrazine ranges from 22.97 to 81.76 days in two soils. The presence of MPs also influenced soil enzyme activities and the effects varied by MP type and soil property. These results demonstrate that MPs can modify the adsorption and persistence of atrazine in soil, thereby increasing its environmental risk. This study provides valuable insights for the long-term ecological risk assessment of co-existing MPs and pesticide pollution in terrestrial environments.","PeriodicalId":48587,"journal":{"name":"Agriculture-Basel","volume":"16 6","pages":"705-705"},"PeriodicalIF":0.0,"publicationDate":"2026-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147877438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Different Conditions on Fermentation Quality and Nutrient Composition of Tender Bamboo Silage After Irradiation","authors":"Jinjiao Chen, Meiqun Li, Baiquan Zeng, Tianqi Wu, Yong Meng, Tengmian Zhou","doi":"10.3390/agriculture16050531","DOIUrl":"https://doi.org/10.3390/agriculture16050531","url":null,"abstract":"The impact of pretreatment methods on the fermentation quality and nutritional profile of bamboo silage was assessed to determine the optimal ensiling strategy. Tender bamboo underwent microwave, ultrasound, alkali, and irradiation pretreatments. Subsequently, a four-factor, three-level L9 (34) orthogonal experiment was employed, utilizing pretreated bamboo as the substrate. The experiment evaluated the effects of silage time (30, 45, 60 days), moisture content (55%, 60%, 65%), cellulase addition [2, 4, and 6 mg/g FM (Fresh weight)], and silage inoculant addition (0.5, 5, 50 mg/g FM). Results indicated that γ-ray irradiation pretreatment effectively reduced lignin and cellulose content while increasing reducing sugars levels approximately fourfold compared to the control group. Six out of the nine treatment groups exhibited superior comprehensive fermentation quality scores, with silage time demonstrating the most significant influence on the fermentation quality of tender bamboo silage. The order of influence was silage time > silage inoculant level > moisture content > cellulase, with a silage time of 30 days, a silage inoculant level of 0.5 mg/g FM, a moisture content of 65%, and a cellulase level of 2 mg/g FM, all contributing to enhanced fermentation quality. Regarding nutritional composition, silage time significantly impacted crude protein and soluble sugar levels, with optimal levels observed at 30 and 60 days, respectively. Moisture content primarily affected soluble sugar levels, followed by neutral detergent fiber, with an optimal level of 55%. Other factors showed minimal effects. Based on fermentation quality and nutritional component analysis, and prioritizing fermentation quality while considering cost-effectiveness, the optimal ensiling conditions for bamboo were determined to be a silage time of 30 days, a moisture content of 65%, an addition of 2 mg/g FM of cellulase, and an addition of 0.5 mg/g FM of silage inoculant.","PeriodicalId":48587,"journal":{"name":"Agriculture-Basel","volume":"16 5","pages":"531-531"},"PeriodicalIF":0.0,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2077-0472/16/5/531/pdf?version=1772199789","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147381934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraction of Sensitive Spectral Bands and Construction of Spectral Indices for Quality Assessment of Fresh Tea Leaves","authors":"Shirui Li, Rui Sun, Yong He, Xiaofeng Li, Yang Li, Liang Zhao, Xinyu Huang, Yufei Xu","doi":"10.3390/agriculture16030360","DOIUrl":"https://doi.org/10.3390/agriculture16030360","url":null,"abstract":"Accurate, nondestructive assessment of fresh tea leaf quality is important for breeding and field management, yet most spectral work still targets processed or low-moisture products. Here, a mechanistically guided hyperspectral method was developed to estimate free amino acids (AA) and total polyphenols (TP) in fresh leaves. Spectral experiments on purified AA and TP powders and their water mixtures identified a key spectral window at 1660 nm. Fractional-order derivatives were applied to leaf reflectance spectra from 102 spring samples (53 varieties), and full-spectrum Partial Least Squares Regression (PLSR) models were used as comparison and validated on an independent set of 40 summer samples. PLSR achieved decent cross-validation coefficient of determination accuracy for AA (Rcv2=0.867) and TP (Rcv2=0.755) and good external prediction coefficient of determination accuracy (RP2=0.793 and 0.776, respectively). Guided by the powder and leaf-level analysis, two-band NDSI indices were derived: the AA index of 1735/1626 nm (R2ₚ = 0.687, RPDₚ = 1.788) and the TP index of 1673/1660 nm (R2ₚ = 0.785, RPDₚ = 2.157) approached the PLSR, indicating that much of the useful information for AA and TP is concentrated in this narrow window and can be captured by simple, interpretable indices potentially suitable for in-field sensing, pending validation across multiple sites, seasons, and management conditions.","PeriodicalId":48587,"journal":{"name":"Agriculture-Basel","volume":"16 3","pages":"360-360"},"PeriodicalIF":0.0,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147381953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of PpZCP11 as a Key Regulator of Primordium Formation in Pleurotus pulmonarius","authors":"Chao Wang, Zhaopeng Ge, W. J. Li, Chao Li, Liudan Wang, Mengfei Chen, Yining Li, Suyue Zheng","doi":"10.3390/agriculture16020211","DOIUrl":"https://doi.org/10.3390/agriculture16020211","url":null,"abstract":"Pleurotus pulmonarius is a high-value, commercially cultivated edible fungus whose primordium formation is a critical phase for yield and commercial value. To better understand the developmental processes of P. pulmonarius, samples from four key developmental stages were collected and subjected to transcriptome analysis. A total of 6530 DEGs were identified, including 50 transcription factors from 10 families. Among these, the PpZCP11 gene, encoding a Zn2Cys6 transcription factor, was found to be specifically highly expressed during the primordium stage. We cloned PpZCP11 gene and confirmed its nuclear localization. The OE-PpZCP11 strains produced abundant primordia, while primordium formation in the RNAi-PpZCP11 strains was severely suppressed. Moreover, RNA-seq and yeast-one-hybrid analysis suggested that PpZCP11 may regulate cell wall synthesis. These findings indicate that the PpZCP11 transcription factor acts as a positive regulator of primordium formation by regulating the expression of cell wall-related genes. This study provides a theoretical reference for elucidating the molecular mechanism underlying primordium formation in P. pulmonarius.","PeriodicalId":48587,"journal":{"name":"Agriculture-Basel","volume":"16 2","pages":"211-211"},"PeriodicalIF":0.0,"publicationDate":"2026-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2077-0472/16/2/211/pdf?version=1768383823","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147913088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}