International Journal of Bioprinting最新文献

{"title":"A 3D-printed micro-perfused culture device with embedded 3D fibrous scaffold for enhanced biomimicry","authors":"Feng Lin Ng, Zhanhong Cen, Y. Toh, L. P. Tan","doi":"10.36922/ijb.0226","DOIUrl":"https://doi.org/10.36922/ijb.0226","url":null,"abstract":"Additive manufacturing has rapidly revolutionized the medical sectors since it is a versatile, cost-effective, assembly free technique with the ability to replicate geometrically complicated features. Some of the widely reported applications include the printing of scaffolds, implants, or microfluidic devices. In this study, a 3D-printed micro-perfused culture (MPC) device embedded with a nanofibrous scaffold was designed to create an integrated micro-perfused 3D cell culture environment for living cells. The addition of 3D fibrous scaffold onto the microfluidic chip was to provide a more physiologically relevant microenvironment for cell culture studies. Stereolithography was adopted in this study as this technique obviates excessive preassembly and bonding steps, which would otherwise be needed in conventional microfluidic fabrication. Huh7.5 hepatocellular carcinoma cells were used as model cells for this platform since liver cells experience similar perfused microenvironment. Preliminary cell studies revealed that gene expressions of albumin (ALB) and cytochrome P450 isoform (CYP3A7) were found to be significantly upregulated on the 3D-printed MPC device as compared to the static counterpart. Taken together, the 3D-printed MPC device is shown to be a physiologically relevant platform for the maintenance of liver cells. The device and printing technique developed in this study is highly versatile and tailorable to mimic local in vivo microenvironment needs of various tissues, which could be studied in future.\u0000 ","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"14 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2023-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91308712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosome-based bioinks for 3D bioprinting applications in tissue engineering and regenerative medicine","authors":"Qian Wang, Yang Liu, Shuqing Zhang, F. He, T. Shi, Jizong Li, Zhimin Wang, Jia Jia","doi":"10.36922/ijb.0114","DOIUrl":"https://doi.org/10.36922/ijb.0114","url":null,"abstract":"Bioprinting is an emerging technology for tissue engineering and regenerative medicine. Despite its fast, accurate manufacture for tissues and organs in vitro, bioprinting has been seriously limited for biofabrication because of the restricted approaches to reproducing the extracellular matrix (ECM) with sufficient bioactivities for bioprinted cells. Exosomes are natural biological particles with proteins, lipids, or genetic materials. They have distinct properties and unique biological functions to manipulate cellular behaviors and cell fates, showing great potential to support cells for bioprinting. Here, we reviewed the recent progresses of exosome-advanced bioprinting for tissue engineering and regenerative medicine. Firstly, we offer an overview of the basics of exosomes and the current representative applications of exosomes in bone tissue engineering, immunological regulations, angiogenesis, and neural regenerations. Then, a brief introduction about the bioinks and the currently developed bioprinting methods is provided. We further give an in-depth review of the biomedical applications of bioprinting with exosomes, majorly in bone engineering, vascular engineering, therapy of neuron injury, and skin regeneration. We also conclude with an outlook on the challenges of the unmet needs of bioprinting cells with correspondent ECM environments through bioprinting with exosomes.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"78 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2023-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88289079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an affordable extrusion 3D bioprinter equipped with a temperature-controlled printhead","authors":"Carlos Ezio Garciamendez-Mijares, Gilberto Emilio Guerra-Alvarez, Mónica Gabriela Sánchez-Salazar1,4, Andrés García-Rubio, Germán García-Martínez, Anne-Sophie Mertgen, C. Ceballos-González, Edna Johana Bolívar-Monsalve, Yu Shrike Zhang, G. Santiago, M. M. Álvarez","doi":"10.36922/ijb.0244","DOIUrl":"https://doi.org/10.36922/ijb.0244","url":null,"abstract":"Bioprinters show great promise as enablers of regenerative medicine and other biomedical engineering applications. In this work, we present a flexible and cost-effective design for a do-it-yourself bioprinter capable of printing/bioprinting gelatin methacryloyl (GelMA) and Pluronic constructs at flow rates of 0.05–0.1 mL/min and effective resolutions of 500–700 μm. The most distinctive feature of this bioprinter is its ability to control the rheology of bioinks simply by adjusting the extrusion temperature during printing. This is achieved by circulating temperature-controlled water within the printhead, which is engineered as a single 3D-printed component consisting of a water-recirculation jacket surrounding the ink/bioink cartridge. The flexibility to circulate either warm or cold water allows the system to be adapted according to the needs dictated by the bioink composition. Herein, we demonstrate the ability to control the printability of GelMA or Pluronic fibers by decreasing or increasing the temperature, respectively, thereby regulating its viscosity. In addition, any commercial needle with a Luer lock can be incorporated into the printhead, allowing the easy fabrication of fibers of different diameters with a single printhead. We showed that our bioprinter is capable of printing simple 2D constructs with high fidelity (i.e., lines of GelMA with a thickness of ~522 ± 36.83 μm can be printed at linear speeds of 100 mm min−1) and 3D constructs composed of as many as five layers of cell-laden 5% GelMA. We also demonstrated that C2C12 cells bioprinted through needle tips (300 μm in diameter) exhibit adequate post-printing viability (~90%), as well as spreading after 7 days of culture. The presentation of this bioprinter may contribute appreciably to the expansion of bioprinter use due to its low overall cost of manufacture, flexibility and open-source character, amenability to modification and adaptation for use with different 3D-printed printheads, and ability to bioprint using GelMA.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"13 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2023-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78090200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D printing of PCL-ceramic composite scaffolds for bone tissue engineering applications","authors":"Santosh Kumar Parupelli, Sheikh Saudi, N. Bhattarai, S. Desai","doi":"10.36922/ijb.0196","DOIUrl":"https://doi.org/10.36922/ijb.0196","url":null,"abstract":"Three-dimensional (3D) printing was utilized for the fabrication of a composite scaffold of poly(ε-caprolactone) (PCL) and calcium magnesium phosphate (CMP) bioceramics for bone tissue engineering application. Four groups of scaffolds, that is, PMC-0, PMC-5, PMC-10, and PMC-15, were fabricated using a custom 3D printer. Rheology analysis, surface morphology, and wettability of the scaffolds were characterized. The PMC-0 scaffolds displayed a smoother surface texture and an increase in the ceramic content of the composite scaffolds exhibited a rougher structure. The hydrophilicity of the composite scaffold was significantly enhanced compared to the control PMC-0. The effect of ceramic content on the bioactivity of fibroblast NIH/3T3 cells in the composite scaffold was investigated. Cell viability and toxicity studies were evaluated by comparing results from lactate dehydrogenase (LDH) and Alamar Blue (AB) colorimetric assays, respectively. The live-dead cell assay illustrated the biocompatibility of the tested samples with more than 100% of live cells on day 3 compared to the control one. The LDH release indicated that the composite scaffolds improved cell attachment and proliferation. In this research, the fabrication of a customized composite 3D scaffold not only mimics the rough textured architecture, porosity, and chemical composition of natural bone tissue matrices but also serves as a source for soluble ions of calcium and magnesium that are favorable for bone cells to grow. These 3D-printed scaffolds thus provide a desirable microenvironment to facilitate biomineralization and could be a new effective approach for preparing constructs suitable for bone tissue engineering.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"3 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2023-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87944021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organoid bioprinting strategy and application in biomedicine: A review","authors":"Chen He, Jiasheng Yan, Yusheng Fu, Jiuchuan Guo, Yuxing Shi, Jinhong Guo","doi":"10.36922/ijb.0112","DOIUrl":"https://doi.org/10.36922/ijb.0112","url":null,"abstract":"Organoids are three-dimensional cell structures cultured in vitro. They are self-organizing and can mimic real organs in structure and function. Bioprinting technology breaks through some limitations of organoid manufacturing, making it more widely used in drug screening, regenerative medicine, and other fields. In this review, we first introduce bioinks and bioprinting methods for stem cell and organoid bioprinting, then summarize several vascularization strategies for bioprinting organoids, and present applications in biomedicine. In the future, the development of microfluidic technology and four-dimensional bioprinting technology may be conducive to forming better bioprinted organoids.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"95 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79649681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D Aerosol Jet® printing for microstructuring: Advantages and limitations","authors":"Miriam Seiti, Olivier Degryse, Rosalba Monica Ferraro, S. Giliani, V. Bloemen, E. Ferraris","doi":"10.36922/ijb.0257","DOIUrl":"https://doi.org/10.36922/ijb.0257","url":null,"abstract":"Aerosol Jet® printing (AJ®P) is a direct writing printing technology that deposits functional aerosolized solutions on free-form substrates. Its potential has been widely adopted for two-dimensional (2D) microscale constructs in printed electronics (PE), and it is rapidly growing toward surface structuring and biological interfaces. However, limited research has been devoted to its exploitation as a three-dimensional (3D) printing technique. In this study, we investigated AJ®P capabilities for 3D microstructuring of three inks, as well as their advantages and limitations by employing three proposed 3D AJ®P strategies (continuous jet deposition, layer-by-layer, and point-wise). In particular, 3D microstructures of increasing complexity based on silver nanoparticle (AgNPs)-, poly(3,4-ethylenedioxythiophene)polystyrene sulfonate (PEDOT:PSS)-, and collagen-based inks were investigated at various aspect ratios and resolutions. Biocompatibility assays were also performed to evaluate inks cytotoxicity effects on selected cellular lineages, including neuronal and osteoblast cell lines. Results show the possibility to print not only arrays of micropillars of different aspect ratios (AgNPs-ARs ~ 20, PEDOT:PSS-ARs ~ 4.5, collagen-ARs ~ 2.5), but also dense and complex (yet low reproducible) leaf- or flake-like structures (especially with the AgNPs-based ink), and lattice units (collagen-based ink). Specifically, this study demonstrates that the fabrication of 3D AJ®-printed microstructures is possible only with a specific set of printing parameters, and firmly depends on the ink (co-)solvents fast-drying phenomena during the printing process. Furthermore, the data concerning inks biocompatibility revealed high cytotoxicity levels for the AgNPs-based ink, while low ones for the PEDOT:PSS and the collagen-based inks. In conclusion, the paper provides general guidelines with respect to ink development and print strategies for 3D AJ®P microstructuring, opening its adoption in a vast range of applications in life science (tissue engineering, bioelectronic interfaces), electronics, and micromanufacturing.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"61 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2023-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84846611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sub-regional design of the bionic bone scaffolds using macrostructural topology","authors":"Yangdong He, Long Chao, Chen Jiao, Hong Wang, Deqiao Xie, Guofeng Wu, Lin Wang, Changjiang Wang, Jianfeng Zhao, Lida Shen, Hui-xin Liang","doi":"10.36922/ijb.0222","DOIUrl":"https://doi.org/10.36922/ijb.0222","url":null,"abstract":"With the increasing demand for bone repair, the bionic bone scaffolds have become a research hotspot. A sub-regional design method of the bionic bone scaffolds, using macrostructural topology, is proposed in this paper, aiming to provide a functionally enhanced region division method for the gradient design. The macrostructural topology was carried out by the bi-directional evolutionary structural optimization (BESO), dividing the predefined design domain into sub-region A and sub-region B. Subsequently, a combined probability sphere model and a distance-to-scale coefficient mapping model are established to implement the graded porosification based on the Voronoi tessellation. This approach takes geometric and mechanical continuity into fully account and assures a reasonable distribution of characteristic parameters, yielding to improve the mechanical strength under specific stress conditions. Finally, the scaffolds were fabricated by the laser powder bed fusion (LPBF) process using the Ti-6Al-4V powder. The results of compression tests are satisfactory, showing that the as-built specimens implement sub-regional functionality. The apparent elastic modulus and the ultimate strength range, respectively, between 1.50 GPa and 7.12 GPa (for the first module) and between 38.55 MPa and 268.03 MPa (for the second module), which conform to the required level of natural bone, providing a possibility for clinical application.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"2014 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87749920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Printed cisplatin on microneedle arrays for transdermal delivery enhances olaparib-induced synthetic lethality in a mouse model of homologous recombination deficiency","authors":"Z. Kanaki, Alexandra Smina, C. Chandrinou, Fotini E. Koukouzeli, Yiannis Ntounias, N. Paschalidis, I. Cheliotis, M. Makrygianni, Jill Ziesmer, Georgios A. Sotiriou, I. Zergioti, C. Tamvakopoulos, A. Klinakis","doi":"10.36922/ijb.0048","DOIUrl":"https://doi.org/10.36922/ijb.0048","url":null,"abstract":"Small molecule inhibitors targeting specific proteins are claiming a continuously growing share in cancer therapy, more commonly in combination with traditional chemotherapeutic drugs. While these inhibitors are taken orally, the majority of chemotherapies are administered through intravenous injection in the hospital premises. Alternative routes for chemotherapy administration would allow more frequent administration at lower dosing by the patient oneself, allowing combination treatment with reduced side effects. Here, we employed laser printing to prepare microneedles for transdermal delivery of cisplatin. Combination treatment with cisplatin transdermally and the poly (ADP-ribose) polymerase (PARP) inhibitor olaparib orally leads to effective treatment in a cancer xenograft mouse model in vivo, while reducing the risk for systemic side effects. This work opens new avenues in anti-cancer therapy by allowing the administration of chemotherapy without the need for intravenous injection alone or in combination with other therapies.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"29 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2023-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80288406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computational fluid dynamics for the optimization of internal bioprinting parameters and mixing conditions","authors":"Gokhan Ates, P. Bártolo","doi":"10.36922/ijb.0219","DOIUrl":"https://doi.org/10.36922/ijb.0219","url":null,"abstract":"Tissue engineering requires the fabrication of three-dimensional (3D) multimaterial structures in complex geometries mimicking the hierarchical structure of biological tissues. To increase the mechanical and biological integrity of the tissue engineered structures, continuous printing of multiple materials through a printing head consisting of a single nozzle is crucial. In this work, numerical analysis was carried out to investigate the extrusion process of two different shear-thinning biomaterial solutions (alginate and gelatin) inside a novel single-nozzle dispensing system consisting of cartridges and a static mixer for varying input pressures, needle geometries, and outlet diameters. Systematic analysis of the dispensing process was conducted to describe the flow rate, velocity field, pressure drop, and shear stress distribution throughout the printing head. The spatial distribution of the biopolymer solutions along the mixing chamber was quantitatively analyzed and the simulation results were validated by comparing the pressure drop values with empirical correlations. The simulation results showed that the proposed dispensing system enables to fabricate homogenous material distribution across the nozzle outlet. The predicted shear stress along the proposed printing head model is lower than the critical shear values which correspond to negligible cell damage, suggesting that the proposed dispensing system can be used to print cell-laden tissue engineering constructs.\u0000 ","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"43 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2023-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83256814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrospinning polyethylene terephthalate glycol","authors":"Mohamed S H Hassan, Abdalla M. Omar, E. Daskalakis, B. Grieve, P. Bártolo","doi":"10.36922/ijb.0024","DOIUrl":"https://doi.org/10.36922/ijb.0024","url":null,"abstract":"Polyethylene terephthalate glycol (PETG) is a difficult-to-spin material, and no previous papers have reported the correct conditions to create PETG meshes. To address this issue, a preliminary study on the solubility and electrospinnability of PETG using a range of solvent system was conducted and a Teas graph was established to select the ideal solvent system. Based on these preliminary results, electrospun PETG fibers were produced using a highly volatile binary solvent system consisting of dichloromethane (DCM) and trifluoroacetic acid (TFA). Produced meshes were extensively characterized, and the results demonstrated for the first time the ability of electrospun PETG meshes to support the inoculation and germination of yellow rust spores, thus confirming that PETG is an ideal material to be used for the fabrication of agriculture biosensors. The results also showed that the best solvent split was 85/15 (DCM/TFA).","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"59 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2023-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80849757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}