International Journal of Bioprinting最新文献

{"title":"Construction of a 3D-printed polycaprolactone scaffold functionalized with extracellular matrix and an MSC-affinity peptide for articular cartilage repair","authors":"Xinqiang Zhao, Xiaoning Liu, Zhen Huang, Kun Ding, Yantao Zhao, Zhenggang Bi","doi":"10.36922/ijb026250258","DOIUrl":"https://doi.org/10.36922/ijb026250258","url":null,"abstract":"Cartilage damage has emerged as a major clinical challenge due to the limited intrinsic regenerative capacity of articular cartilage. Current cartilage repair strategies often rely on exogenous cells and growth factors to promote tissue regeneration; however, their clinical translation is hindered by high costs, complex manufacturing processes, and potential immunogenicity. Thus, the development of high-performance acellular tissue-engineering scaffolds has attracted increasing attention. In this study, we engineered a biomimetic ternary composite scaffold consisting of polycaprolactone (PCL), a bone marrow mesenchymal stem cell (BMSC)-affinity peptide, and cartilage extracellular matrix (ECM) to facilitate efficient cartilage regeneration through synergistic multifunctional effects. PCL scaffolds with well-defined porous architectures and favorable mechanical properties were fabricated using three-dimensional (3D) printing. Following surface functionalization with the mesenchymal stem cell (MSC)-affinity peptide E7, the scaffolds were further coated with decellularized cartilage ECM. This biomimetic design enhances cell adhesion, selectively recruits endogenous MSCs, and provides the structural support required for tissue regeneration. Moreover, the bioactive components and biochemical cues retained within the ECM promote chondrogenic differentiation. In vitro studies demonstrated that E7-modified PCL (E7-PCL) significantly enhanced MSC adhesion and recruitment. The ECM/E7-modified composite scaffold further promoted stem-cell adhesion, proliferation, chondrogenic differentiation, and extracellular matrix deposition while maintaining excellent biocompatibility and mechanical integrity. In vivo, implantation of the ECM/E7-PCL (E/E7-P) scaffold resulted in markedly improved cartilage repair compared with ECM/PCL (E/P) scaffolds and untreated controls. Overall, these findings from in vitro and rabbit in vivo experiments indicate that E/E7-P represents a promising acellular tissue-engineering candidate for cartilage defect regeneration, pending further validation in more comprehensive experimental models.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"0 0","pages":"026250258-026250258"},"PeriodicalIF":0.0,"publicationDate":"2026-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://article.accscience.com/journal/IJB/online_first/ijb026250258/article_ijb0262502581_593327.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148672254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-nozzle 3D-printed calcium sulfate/poly (lactic acid) scaffold with linezolid microspheres for efficient repair of femoral condyle defects in rats","authors":"Xiaojie Tang, Chenxu Li, Yanan Wang, Hai Huang, Tongshuai Xu, Shannan Cao, Chengxin Lv, Xiaofan Du, Shuqing Chen, Wenkang Yang, Jiale Shao, Yukun Du, Yongming Xi","doi":"10.36922/ijb025270269","DOIUrl":"https://doi.org/10.36922/ijb025270269","url":null,"abstract":"Large complex bone defects pose a significant clinical challenge. Conventional bone grafting approaches cannot simultaneously achieve tissue regeneration and infection prevention, resulting in impaired healing outcomes and prolonged treatment cycles. Existing therapeutic strategies lack integrated solutions capable of concurrently providing infection prevention and osteogenesis promotion within a single platform. This study developed a novel multifunctional composite scaffold using dual-nozzle 3D printing technology to simultaneously achieve infection prevention and accelerated bone regeneration. Linezolid-loaded poly(lactic-co-glycolic acid) microspheres (LMS) were uniformly dispersed within the pores of Calcium sulfate/polylactic acid (CS/PLA) scaffolds to successfully construct the composite scaffold. In vitro characterization revealed uniform distribution of microspheres within the scaffold pores, with the fabricated CS/PLA-LMS demonstrating excellent biocompatibility and mechanical properties, achieving an elastic modulus of 87 MPa. Furthermore, the composite scaffold effectively inhibited Staphylococcus aureus activity in vitro. In vivo studies in a rat cranial defect model revealed that the composite scaffold significantly enhanced bone formation compared to blank controls, bone volume fraction increased by 3.2 times, and trabecular spacing decreased by 50%, with mechanistic analysis indicating activation of the PI3K-AKT signaling pathway. The integrated design successfully prevented infection-related complications while promoting robust osteogenesis, offering a clinically relevant solution for treating complex bone defects where infection prevention and regenerative capacity are primary therapeutic concerns.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"0 0","pages":"025270269-025270269"},"PeriodicalIF":0.0,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147877969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Macro-Micro analysis of scaffold properties and study of biological properties of 3D printed hydroxyapatite/β-Tricalcium phosphate scaffolds influenced by polyvinyl alcohol concentration","authors":"Zhitao Yin, Yutong Chen, Guang Yang, Shuaishuai Wang, Bingbing Wang, Yue Zhao, Xujing Zhang, Yan Xu","doi":"10.36922/ijb025280274","DOIUrl":"https://doi.org/10.36922/ijb025280274","url":null,"abstract":"The concentration of the binder is a key factor affecting the quality of 3D-printed bone scaffolds. This study analyzed the influence of polyvinyl alcohol (PVA) aqueous solution concentration on the properties of hydroxyapatite (HA)/β-tricalcium phosphate (β-TCP) bone scaffolds from both microscopic and macroscopic perspectives using molecular dynamics (MD) simulations and experimental research. In the MD simulations, the changes in chain length corresponding to different concentrations were used to analyze the microscopic interactions between PVA and the powder material system. In the experiments, the solid content, zeta potential, and extrusion rheological properties of the slurry were analyzed under PVA concentrations ranging from 5% to 15% by mass. Bone scaffolds were then fabricated using 3D printing and freeze-drying processes, and the changes in porosity, mechanical properties, dimensional shrinkage, and swelling effect of the scaffolds were examined. Finally, the biological properties of the scaffolds were verified through in vitro experiments. The results showed that the hydrogen bonds and ionic bonds formed between PVA and the powder materials are the main forces for adhesion, and the increase in chain length, which leads to an increase in Cauchy pressure, enhances the basic mechanical properties of the material. Slurries with higher PVA concentrations have higher solid content and shear-thinning capabilities, ensuring better printability, and the resulting bone scaffolds exhibit higher mechanical properties and drying shrinkage characteristics. However, this also leads to lower porosity and swelling rates. In vitro experiments revealed that an increase in PVA aqueous solution concentration results in decreased porosity and ion concentration of the bone scaffolds, thereby reducing their bioactivity. The conclusions drawn from this study can be used to predict the performance of slurries and bone scaffolds at different binder concentrations, providing a theoretical basis for the selection of binder concentration in 3D-printed bone scaffolds.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"0 0","pages":"025280274-025280274"},"PeriodicalIF":0.0,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147879179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing Modular Microfluidics: Stereolithographic 3D Printing of Reconfigurable Connectors for Bioanalytical Applications.","authors":"Hong-Wei Zhang, Clara Tamura, Alireza Ahmadianyazdi, Albert Folch, Ting-Yuan Tu","doi":"10.36922/ijb025140125","DOIUrl":"10.36922/ijb025140125","url":null,"abstract":"<p><p>Traditional monolithic microfluidic devices are constrained by their inability to accommodate modifications to circuit elements, necessitating complete redesign and refabrication. To address these limitations, this study introduces modular microfluidic connectors fabricated via stereolithographic (SL) 3D printing. We designed and evaluated three distinct connector types-tessellated, sponge, and solid-walled-using tailored photoresins to enhance reusability, flexibility, and sealing performance. The tessellated connectors, printed with poly(ethylene glycol) diacrylate (PEGDA, Mw ~258) and incorporating an Octet Unit cell structure, reduced the rigidity of PEGDA prints, improving reusability under moderate conditions. The sponge connectors, fabricated from a PEGDA and 2-hydroxyethyl acrylate (2-HEA, Mw ~116) blend (2-HEA-co-PEGDA), exhibited greater flexibility; however, swelling in aqueous environments may limit their long-term utility. In contrast, the solid-walled connectors, produced with commercial Asiga Soft Resin, demonstrated superior reliability and adaptability, as validated in a reconfigurable concentration gradient generator (CGG) with scalable output capabilities. Cytocompatibility tests confirmed that PEGDA-printed devices, following isopropanol (IPA) and UV post-processing, are suitable for bioanalytical applications that do not require incubation. These findings establish SL 3D printing as a promising method for developing flexible, reconfigurable microfluidic platforms, with potential uses in material synthesis, chemical analysis, and point-of-care diagnostics. While challenges related to environmental durability persist, these advances lay the foundations for developing more robust and adaptable microfluidic systems with versatile applications.</p>","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"11 3","pages":"516-531"},"PeriodicalIF":5.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12782180/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145953676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological 3D-printed viscoelastic scaffolds with controllable stress relaxation rates","authors":"Liqin Zhao, Danyu Yao, Zhenhai Xia, Ling Wang, Mingen Xu","doi":"10.36922/ijb025090074","DOIUrl":"https://doi.org/10.36922/ijb025090074","url":null,"abstract":"&amp;nbsp;The natural extracellular matrix (ECM) exhibits remarkable viscoelasticity and stress relaxation. Constructing viscoelastic scaffolds that can precisely control the stress relaxation rate and possess good biocompatibility is a key challenge in the design of tissue engineering scaffolds. Understanding the factors influencing the viscoelasticity of scaffolds and their mechanisms, as well as implementing comprehensive regulatory strategies based on this understanding, are effective methods for precisely controlling the stress relaxation rate. Current research on viscoelastic scaffolds mainly focuses on the regulation of bulk hydrogel viscoelasticity, while the impact of 3D printing parameters on stress relaxation time remains underexplored. In this study, we controlled the structure and morphology of silk fibroin to obtain a crystalline silk fibroin fiber (SL) solution, which was then mixed with gelatin solution to achieve high-precision printing of low-concentration (&lt;2%) silk fibroin. Based on this, we explored the effects of printing angle, fiber diameter, and porosity on the stress relaxation rate and elastic modulus of the scaffold. Specifically, as porosity increases, the relaxation rate tends to rise, while the elastic modulus decreases. Conversely, as the printing angle and fiber diameter increase, the relaxation rate significantly decreases, and the elastic modulus correspondingly increases. We verified these effects using alginate-based bioink, demonstrating the universality of the influence of printing parameters on scaffold viscoelasticity. Additionally, we constructed scaffolds with similar elastic moduli but different stress relaxation rates and investigated their effects on cell growth, thereby confirming the good biocompatibility of viscoelastic scaffolds. This study not only provides a theoretical basis for precisely controlling the stress relaxation rate of 3D-printed viscoelastic scaffolds but also offers new insights for the design and optimization of tissue engineering scaffolds.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"0 0","pages":"025090074-025090074"},"PeriodicalIF":0.0,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147330366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a 3D-printed polycaprolactone/magnesium phosphate composite scaffold functionalized with novel antimicrobial peptides for enhanced bone defect regeneration","authors":"Ling Zheng, Miao Li, Chen Liang, Zu Wei, Ying Zhao","doi":"10.36922/ijb025080060","DOIUrl":"https://doi.org/10.36922/ijb025080060","url":null,"abstract":"The treatment of large-sized infectious bone defects is currently one of the most urgent clinical challenges that need to be addressed in clinical practice. The clinical application of autologous and allogeneic bone grafts faces numerous persistent challenges that remain unresolved. Therefore, there is an urgent need to develop a bone repair scaffold capable of large-scale production, safe for in vivo use, and possessing robust bone repair and anti-infective properties. In this study, a 3D-printed bone repair scaffold was fabricated using a polycaprolactone (PCL) and magnesium phosphate (MgP) composite material. The scaffold subsequently underwent surface modification with the antimicrobial peptide Tet213 with a DOPA tail, ultimately leading to the development of a novel bone repair scaffold named DTet213@PCL/MgP. The experimental results demonstrated that the DTet213@PCL/MgP scaffold exhibited outstanding antibacterial efficacy against&amp;nbsp;Escherichia coli&amp;nbsp;(E.coli) and&amp;nbsp;Staphylococcus aureus&amp;nbsp;(S.aureus), along with superior proliferation and osteogenesis capabilities for MC3T3-E1 preosteoblastic cells. In a rat radial defect model, the scaffold effectively induced new bone formation at the defect site, resulting in rapid bone regeneration. Furthermore, histopathological examination (H&amp;E staining) of major organs confirmed the excellent in vivo biocompatibility and safety profile of the DTet213@PCL/MgP scaffold. In the future, the DTet213@PCL/MgP scaffold represents a novel solution for the treatment of large-scale infected bone defects, capitalizing on its dual functionality in osteogenesis and infection control.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"0 0","pages":"025080060-025080060"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147896490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toward robust and reproducible pluripotent stem cell expansion in bioprinted GelMA constructs.","authors":"Elizabeth R Komosa, Wei-Han Lin, Brenda M Ogle","doi":"10.36922/ijb.4633","DOIUrl":"https://doi.org/10.36922/ijb.4633","url":null,"abstract":"<p><p>Combining the technologies of 3D bioprinting and human induced pluripotent stem cells (hiPSCs) has allowed for the creation of tissues with organ-level function in the lab, a promising technique for disease modeling and regenerative medicine. Expanding the stem cells in bioprinted tissues prior to differentiation allows for high cell density, which is important for the formation of cell-cell junctions necessary for macroscale function upon differentiation. Yet, stem cell expansion, critical to successful <i>in situ</i> differentiation, depends heavily on the composition of the bioprinted scaffold. Here, we demonstrate how a common bioink component, gelatin methacryloyl (GelMA), varies depending on the vendor and degree of functionalization. We found that the vendor/GelMA production technique played a greater role in dictating the mechanical properties of the bioprinted constructs than the degree of functionalization, emphasizing the importance of reporting detailed characterization of GelMA scaffolds. Furthermore, the ability of singularized hiPSCs to survive and expand in GelMA scaffolds greatly varied across batches from different vendors and degrees of functionalization, where expansion correlated with the mechanical properties of the scaffold. Yet, we found that using a commercial cloning supplement could restore the ability of single hiPSCs to survive and expand across GelMA types, thus compensating for the varied mechanical properties of the scaffolds. These findings provide a practical guide for the expansion of hiPSCs in GelMA constructs with various mechanical properties as required for successful <i>in situ</i> differentiation.</p>","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"11 1","pages":"363-381"},"PeriodicalIF":6.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12052315/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144054711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrospun polylactic acid-glycolic acid composite hydrogel scaffold loaded with 3D extracellular vesicles for nasal septal cartilage defect repair","authors":"Jie Yang, Haolei Hu, Qiang Guo, Xiaolei Chen, Shuo Li, Gang Yin, Wei Yue, Yi Zhang, Boxun Liu, Jianwei Chen, Tao Xu, Yi Li","doi":"10.36922/ijb.4118","DOIUrl":"https://doi.org/10.36922/ijb.4118","url":null,"abstract":"The nasal septum plays an important role in the growth and support of the human nose, and defects can cause nasal deformities. Extracellular vesicles (EVs) have demonstrated great potential in tissue repair. Stem cell EVs are widely used in the repair of articular cartilage defects, but their use for nasal septal cartilage defects has not been reported. Due to the low yield and loss of EVs during in situ injection, improved preparation methods and better carriers are needed for the effective sustained release of EVs in wounds. In this study, swelling and degradation experiments were initially conducted on the scaffold, along with mechanical performance testing, including observation of the scaffold morphology using scanning electron microscopy (SEM). Subsequently, in vitro cell experiments were conducted to evaluate the ability of 3D EVs to promote chondrocyte proliferation, migration, and extracellular matrix formation. Finally, the EV-laden gelatin methacrylic acid-polylactic acid-glycolic acid (Gel-PLGA) composite scaffold was implanted into the nasal septum defect site of rabbits in vivo to observe its repair effect on the defect. In vitro experiments demonstrated that the biological scaffold exhibited good biocompatibility and could effectively promote the proliferation and migration of chondrocytes. In vivo, the EV-laden composite biological scaffold was implanted into the nasal septum defect of rabbits, and the tissues were tested at 6 and 12 weeks after surgery. The results indicate that the composite scaffolds effectively facilitated the repair of defect sites. Taken together, 3D EVs facilitate tissue repair and healing, offering a novel approach to treating nasal septal defects.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"0 0","pages":"4118-4118"},"PeriodicalIF":0.0,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147911943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism study of structural functional dual biomimetic composite bone scaffold for repair of mandibular defects in rabbits","authors":"Jiaqi Hu, Hong Zhu, Chongqing He, Xiaochuan Liu, Kenny Man, Shanqin Liang, Jingying Zhang","doi":"10.36922/ijb.4147","DOIUrl":"https://doi.org/10.36922/ijb.4147","url":null,"abstract":"Objectives: Mandibular issues resulting from injury, gum disease, or congenital defects can restrict chewing ability, impair speech, and cause facial disfigurement, significantly affecting the patient's quality of life. In our previous studies, we investigated the optimum porosity of triply periodic minimal surfaces (TPMS) scaffolds and verified the clinical potential of TPMS scaffolds containing injectable platelet-rich fibrin (I-PRF) and stromal cell-derived factor-1 (SDF-1) (SIT scaffold). This study focuses on the potential mechanisms of SIT bone tissue engineering scaffolds. Methods: Surface structures with a 70% porosity were created using the Matlab R2020a program. Scaffolds were fabricated using digital laser processing. To evaluate the effects on treated F-actin tissues, rabbit bone marrow mesenchymal stem cells were inoculated on SIT scaffolds for 14 days. The morphological changes of individual cells were photographed at 60x magnification using confocal microscopy and quantified with ImageJ software. Histological structures were observed using ALP immunohistochemical staining, Alcian blue, and Masson-Goldner staining. Double-end sequencing in PE150 mode was performed using the Illumina NovaSeq&amp;trade; 6000 according to standard procedures. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), protein-protein interaction (PPI), and gene set variation analysis (GSVA) were conducted to support the interpretation of gene functions. RT-qPCR was performed to confirm the expression of genes of interest. Finally, cells on different samples were examined by confocal laser scanning microscopy to observe c-Jun expression. Results: Confocal imaging and quantitative analysis showed that bone marrow mesenchymal stem cells supported by the SIT scaffold had a greater tendency to differentiate into osteogenesis than those supported by the T scaffold or the control group. The SIT group exhibited the most intense ALP staining. The expression of angiogenesis-related factors VEGFA was significantly upregulated in T and SIT groups. Osteogenic gene RUNX2 and c-Fos/c-Jun pathway genes FOS/JUN were significantly upregulated in the SIT group. GSVA revealed that the WNT signaling pathway and MAPK signaling pathway were more active in the SIT group. Gene expression was validated by qRT-PCR. Immunofluorescence showed that the c-Jun is highly expressed in newly formed capillaries in the SIT group. &amp;nbsp;","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"0 0","pages":"4147-4147"},"PeriodicalIF":0.0,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147919377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Designing a 3D-printed medical implant with mechanically macrostructural topology and microbionic lattices: A novel wedge-shaped spacer for high tibial osteotomy and biomechanical study","authors":"Hsuan-Wen Wang, Chih-Hwa Chen, Kuan-Hao Chen, Yu-Hui Zeng, Chun-Li Lin","doi":"10.36922/ijb.1584","DOIUrl":"https://doi.org/10.36922/ijb.1584","url":null,"abstract":"Metal three-dimensional (3D) printing has become an important manufacturing process in medical implant development. Nevertheless, the metal 3D-printed implant needs to be considered with structural optimization to reduce the stress-shielding effects and to be incorporated with a lattice design to generate better bone ingrowth environment. This study combines topology optimization (TO) and lattice design to acquire an optimal wedge-shaped spacer (OWS) for high tibial osteotomy (HTO) fixation. The OWS was manufactured using titanium alloy 3D printing to conduct biomechanical fatigue testing for mechanical performance validation. A solid wedge-shaped spacer (SWS) with three embedded screws was designed using the HTO model. An OWS was obtained under physiological loads through finite element (FE) analysis and TO. A deformed YM lattice with a porosity of 60% and pore size of 700 μm was filled at the OWS posterior region. The HTO mechanical performance was simulated for SWS, OWS, and commercial T-shaped plate (TP) fixations using FE analysis. The displacement/fracture patterns under OWS and TP fixations were verified using fatigue testing. The manufacturing errors for all 3D-printed OWS features were found to be less than 1%. The FE results revealed that the OWS fixation demonstrated reductions of 56.46%, 11.98%, and 64.31% in displacement, stress in the implant and bone, respectively, compared to the TP fixation. The fatigue test indicated that the OWS fixation exhibited smaller displacement for the HTO, as well as a higher load capacity, minor bone fracture collapse, and a greater number of cycles than the TP system. This study concluded that medical implants can be designed by integrating macro TO and microlattice design to provide enough mechanical strength and an environment for bone ingrowth after surgery. Both FE analysis and biomechanical fatigue tests confirmed that OWS mechanical performance with lattice design was more stable than the HTO TP fixations.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"41 12","pages":""},"PeriodicalIF":8.4,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139441148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}