International Journal of Bioprinting最新文献

{"title":"Error assessment and correction for extrusion-based bioprinting using computer vision method.","authors":"Changxi Liu,&nbsp;Chengliang Yang,&nbsp;Jia Liu,&nbsp;Yujin Tang,&nbsp;Zhengjie Lin,&nbsp;Long Li,&nbsp;Hai Liang,&nbsp;Weijie Lu,&nbsp;Liqiang Wang","doi":"10.18063/ijb.v9i1.644","DOIUrl":"https://doi.org/10.18063/ijb.v9i1.644","url":null,"abstract":"<p><p>299Bioprinting offers a new approach to addressing the organ shortage crisis. Despite recent technological advances, insufficient printing resolution continues to be one of the reasons that impede the development of bioprinting. Normally, machine axes movement cannot be reliably used to predict material placement, and the printing path tends to deviate from the predetermined designed reference trajectory in varying degrees. Therefore, a computer vision-based method was proposed in this study to correct trajectory deviation and improve printing accuracy. The image algorithm calculated the deviation between the printed trajectory and the reference trajectory to generate an error vector. Furthermore, the axes trajectory was modified according to the normal vector approach in the second printing to compensate for the deviation error. The highest correction efficiency that could be achieved was 91%. More significantly, we discovered that the correction results, for the first time, were in a normal distribution instead of a random distribution.</p>","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"9 1","pages":"644"},"PeriodicalIF":8.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7a/cd/IJB-9-1-644.PMC9947486.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9356441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toward better drug development: Three-dimensional bioprinting in toxicological research.","authors":"Diána Szűcs,&nbsp;Zsolt Fekete,&nbsp;Melinda Guba,&nbsp;Lajos Kemény,&nbsp;Katalin Jemnitz,&nbsp;Emese Kis,&nbsp;Zoltán Veréb","doi":"10.18063/ijb.v9i2.663","DOIUrl":"https://doi.org/10.18063/ijb.v9i2.663","url":null,"abstract":"<p><p>The importance of three-dimensional (3D) models in pharmacological tests and personalized therapies is significant. These models allow us to gain insight into the cell response during drug absorption, distribution, metabolism, and elimination in an organ-like system and are suitable for toxicological testing. In personalized and regenerative medicine, the precise characterization of artificial tissues or drug metabolism processes is more than crucial to gain the safest and the most effective treatment for the patients. Using these 3D cell cultures derived directly from patient, such as spheroids, organoids, and bioprinted structures, allows for testing drugs before administration to the patient. These methods allow us to select the most appropriate drug for the patient. Moreover, they provide chance for better recovery of patients, since time is not wasted during therapy switching. These models could be used in applied and basic research as well, because their response to treatments is quite similar to that of the native tissue. Furthermore, they may replace animal models in the future because these methods are cheaper and can avoid interspecies differences. This review puts a spotlight on this dynamically evolving area and its application in toxicological testing.</p>","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"9 2","pages":"663"},"PeriodicalIF":8.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/dd/72/IJB-9-2-663.PMC10090537.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9373137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and characterization of 3D-printed antibacterial hydrogel with benzyl isothiocyanate.","authors":"Yunxia Liang,&nbsp;Bimal Chitrakar,&nbsp;Zhenbin Liu,&nbsp;Xujia Ming,&nbsp;Dan Xu,&nbsp;Haizhen Mo,&nbsp;Chunyang Shi,&nbsp;Xiaolin Zhu,&nbsp;Liangbin Hu,&nbsp;Hongbo Li","doi":"10.18063/ijb.v9i2.671","DOIUrl":"https://doi.org/10.18063/ijb.v9i2.671","url":null,"abstract":"<p><p>Benzyl isothiocyanate (BITC) is an isothiocyanate of plant origin, especially the mustard family, which has good antibacterial properties. However, its applications are challenging due to its poor water solubility and chemical instability. We used food hydrocolloids, including xanthan gum, locust bean gum, konjac glucomannan, and carrageenan as three-dimensional (3D)-printing food ink base and successfully prepared 3D-printed BITC antibacterial hydrogel (BITC-XLKC-Gel). The characterization and fabrication procedure of BITC-XLKC-Gel was studied. The results show that BITC-XLKC-Gel hydrogel has better mechanical properties by low-field nuclear magnetic resonance (LF-NMR), mechanical properties, and rheometer analysis. The strain rate of BITC-XLKC-Gel hydrogel is 76.5%, which is better than that of human skin. Scanning electron microscope (SEM) analysis showed that BITC-XLKC-Gel has uniform pore size and provides a good carrier environment for BITC carriers. In addition, BITC-XLKC-Gel has good 3D-printing performance, and 3D printing can be used for customizing patterns. Finally, inhibition zone analysis showed that the BITC-XLKC-Gel added with 0.6% BITC had strong antibacterial activity against <i>Staphylococcus aureus</i> and the BITC-XLKC-Gel added with 0.4% BITC had strong antibacterial activity against <i>Escherichia coli</i>. Antibacterial wound dressing has always been considered essential in burn wound healing. In experiments that simulated burn infection, BITC-XLKC-Gel showed good antimicrobial activity against methicillin-resistant <i>S. aureus</i>. BITC-XLKC-Gel is a good 3D-printing food ink attributed to strong plasticity, high safety profile, and good antibacterial performance and has great application prospects.</p>","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"9 2","pages":"671"},"PeriodicalIF":8.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3d/ce/IJB-9-2-671.PMC10090813.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9373140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-dimensional printing of microfiber- reinforced hydrogel loaded with oxymatrine for treating spinal cord injury.","authors":"Shiqiang Song,&nbsp;Jing Zhou,&nbsp;Junming Wan,&nbsp;Xingchang Zhao,&nbsp;Kai Li,&nbsp;Chengliang Yang,&nbsp;Chuanchuan Zheng,&nbsp;Liqiang Wang,&nbsp;Yujin Tang,&nbsp;Chong Wang,&nbsp;Jia Liu","doi":"10.18063/ijb.692","DOIUrl":"https://doi.org/10.18063/ijb.692","url":null,"abstract":"<p><p>Spinal cord injury (SCI) causes severe neural tissue damage and motor/sensory dysfunction. Since the injured spinal cord tissue has limited self-regeneration ability, several strategies, including cell therapy, drug delivery, and tissue engineering scaffold implantation, have been employed to treat SCI. However, each of these strategies fails to obtain desirable outcomes due to their respective limitations. In comparison, advanced tissue engineering scaffolds with appropriate topographical features, favorable composition, and sustained drug delivery capability can be employed to recruit endogenous neural stem cells (NSCs), induce neuronal differentiation, and facilitate neuron maturation. This can lead to the regeneration of injured spinal cord tissue and the recovery of motor function. In this study, fiber bundle-reinforced spinal cord extracellular matrix hydrogel scaffolds loaded with oxymatrine (OMT) were produced through nearfield direct write electrospinning. The spinal cord extracellular matrix-based hydrogel was then coated with OMT. The physical/chemical properties and <i>in vitro</i> degradation behavior of the composite scaffolds were investigated. The <i>in vitro</i> cell culture results showed that composite scaffolds loaded with OMT promoted the differentiation of NSCs into neurons and inhibited differentiation into astrocytes. The <i>in vivo</i> results showed that the composite scaffolds loaded with OMT recruited NSCs from the host tissue, promoted neuronal differentiation and axon extension at the lesion site, inhibited glial scar formation at/around the lesion site, and improved the recovery of motor function in rats with SCI. To sum up, 3D-printed microfiber-reinforced spinal cord extracellular matrix hydrogel scaffolds loaded with OMT are promising biomaterials for the treatment of SCI.</p>","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"9 3","pages":"692"},"PeriodicalIF":8.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fe/e6/IJB-9-3-692.PMC10236342.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9578369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Up-to-date progress in bioprinting of bone tissue.","authors":"Yang Wu,&nbsp;Ming Li,&nbsp;Hao Su,&nbsp;Huaying Chen,&nbsp;Yonggang Zhu","doi":"10.18063/ijb.v9i1.628","DOIUrl":"https://doi.org/10.18063/ijb.v9i1.628","url":null,"abstract":"<p><p>The major apparatuses used for three-dimensional (3D) bioprinting include extrusion-based, droplet-based, and laser-based bioprinting. Numerous studies have been proposed to fabricate bioactive 3D bone tissues using different bioprinting techniques. In addition to the development of bioinks and assessment of their printability for corresponding bioprinting processes, <i>in vitro</i> and <i>in vivo</i> success of the bioprinted constructs, such as their mechanical properties, cell viability, differentiation capability, immune responses, and osseointegration, have been explored. In this review, several major considerations, challenges, and potential strategies for bone bioprinting have been deliberated, including bioprinting apparatus, biomaterials, structure design of vascularized bone constructs, cell source, differentiation factors, mechanical properties and reinforcement, hypoxic environment, and dynamic culture. In addition, up-to-date progress in bone bioprinting is summarized in detail, which uncovers the immense potential of bioprinting in re-establishing the 3D dynamic microenvironment of the native bone. This review aims to assist the researchers to gain insights into the reconstruction of clinically relevant bone tissues with appropriate mechanical properties and precisely regulated biological behaviors.</p>","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"9 1","pages":"628"},"PeriodicalIF":8.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a7/f1/IJB-9-1-628.PMC9830997.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10525799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hybrid biomanufacturing systems applied in tissue regeneration.","authors":"Fengyuan Liu,&nbsp;Rixiang Quan,&nbsp;Cian Vyas,&nbsp;Enes Aslan","doi":"10.18063/ijb.v9i1.646","DOIUrl":"https://doi.org/10.18063/ijb.v9i1.646","url":null,"abstract":"<p><p>Scaffold-based approach is a developed strategy in biomanufacturing, which is based on the use of temporary scaffold that performs as a house of implanted cells for their attachment, proliferation, and differentiation. This strategy strongly depends on both materials and manufacturing processes. However, it is very difficult to meet all the requirements, such as biocompatibility, biodegradability, mechanical strength, and promotion of cell-adhesion, using only single material. At present, no single bioprinting technique can meet the requirements for tissue regeneration of all scales. Thus, multi-material and mixing-material scaffolds have been widely investigated. Challenges in terms of resolution, uniform cell distribution, and tissue formation are still the obstacles in the development of bioprinting technique. Hybrid bioprinting techniques have been developed to print scaffolds with improved properties in both mechanical and biological aspects for broad biomedical engineering applications. In this review, we introduce the basic multi-head bioprinters, semi-hybrid and fully-hybrid biomanufacturing systems, highlighting the modifications, the improved properties and the effect on the complex tissue regeneration applications.</p>","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"9 1","pages":"646"},"PeriodicalIF":8.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/17/31/IJB-9-1-646.PMC9831066.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10526271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formulation and evaluation of a bioink composed of alginate, gelatin, and nanocellulose for meniscal tissue engineering.","authors":"Julia Anna Semba,&nbsp;Adam Aron Mieloch,&nbsp;Ewa Tomaszewska,&nbsp;Piotr Cywoniuk,&nbsp;Jakub Dalibor Rybka","doi":"10.18063/ijb.v9i1.621","DOIUrl":"https://doi.org/10.18063/ijb.v9i1.621","url":null,"abstract":"<p><p>1The necessity to preserve meniscal function prompts the research and development of novel treatment options, like three-dimensional (3D) bioprinting. However, bioinks for meniscal 3D bioprinting have not been extensively explored. Therefore, in this study, a bioink composed of alginate, gelatin, and carboxymethylated cellulose nanocrystal (CCNC) was formulated and evaluated. Firstly, bioinks with varying concentrations of the aforementioned components were subjected to rheological analysis (amplitude sweep test, temperature sweep test, and rotation). The optimal bioink formulation of 4.0% gelatin, 0.75% alginate, and 1.4% CCNC dissolved in 4.6% D-mannitol was further used for printing accuracy analysis, followed by 3D bioprinting with normal human knee articular chondrocytes (NHAC-kn). The encapsulated cells' viability was > 98%, and collagen II expression was stimulated by the bioink. The formulated bioink is printable, stable under cell culture conditions, biocompatible, and able to maintain the native phenotype of chondrocytes. Aside from meniscal tissue bioprinting, it is believed that this bioink could serve as a basis for the development of bioinks for various tissues.</p>","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"9 1","pages":"621"},"PeriodicalIF":8.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/28/cb/IJB-9-1-621.PMC9947383.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9340842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"1Biomaterial inks for extrusion-based 3D bioprinting: Property, classification, modification, and selection.","authors":"Li Xiaorui,&nbsp;Zheng Fuyin,&nbsp;Wang Xudong,&nbsp;Geng Xuezheng,&nbsp;Zhao Shudong,&nbsp;Liu Hui,&nbsp;Dou Dandan,&nbsp;Leng Yubing,&nbsp;Wang Lizhen,&nbsp;Fan Yubo","doi":"10.18063/ijb.v9i2.649","DOIUrl":"https://doi.org/10.18063/ijb.v9i2.649","url":null,"abstract":"<p><p>Three-dimensional (3D) extrusion-based bioprinting is the most widely used bioprinting technology to fabricate bionic tissue or organ constructs by combining biomaterial ink and living cells for tissue engineering and regenerative medicine. One critical issue of this technique is the selection of suitable biomaterial ink to simulate extracellular matrix (ECM) that provides mechanical support for cells and regulates their physiological activities. Previous studies have demonstrated that it is an enormous challenge to form and maintain reproducible 3D constructs and eventually achieve the balance among biocompatibility, mechanical properties, and printability. This review highlights the properties of extrusion-based biomaterial inks and recent developments as well as details various biomaterial inks classified by their function. Key approaches related to their modification methods according to the functional requirements are also discussed, along with the selection strategies by varying extrusion paths and methods in extrusion-based bioprinting. This systematical review will assist researchers in identifying the most suitable extrusion-based biomaterial inks based on their requirements, as well as in elaborating current challenges and prospects of extrudable biomaterial inks in the field of bioprinting of in vitro tissue models.</p>","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"9 2","pages":"649"},"PeriodicalIF":8.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/88/a0/IJB-9-2-649.PMC10090818.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9490793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Printing channels with millimeter-scale curvature and deciphering their effect on the proliferation, morphology, orientation, and migration of M-22 cells.","authors":"Huinan Lai,&nbsp;Yuye Huang,&nbsp;Jun Yin,&nbsp;Jin Qian","doi":"10.18063/ijb.681","DOIUrl":"https://doi.org/10.18063/ijb.681","url":null,"abstract":"<p><p>Complex curved structures of tissues have been recognized to influence the behavior and function of cells. Tissue curvatures sensed by cells are approximately on the millimeter scale. However, previous research mainly focused on the effect of micro- and nano-scale spatial curved structures, underestimating the significance of milli-scale curvature. Here, we employed fused deposition modeling (FDM) with two-stage temperature control, superfine cone-shaped needle, stable air pressure, and precise motion platform for the customized production of homogeneous, precise, and curved fibers; the responses of M-22 cells to FDM-printed curved channels with radii of 1.5 to 3 mm were systematically investigated. The cells aligned with these curved channels and exhibited various aspect ratios in the channels with different curvatures. Cell proliferation, migration speed of single cells, and front-end speed of collective cells were tightly regulated by these curved structures. Also, a computational model based on force equilibrium was proposed to explore the essential factors and mechanisms of curvature affecting cell behavior. Our simulation results demonstrated that the curvature and width of channels, along with the relative size of cells, can significantly impact the cell-boundary interaction force and the number of valid pseudopodia generated by cells in the process of cell migration. These results provide a comprehensive understanding of the effect of milli-scale curvature on the cells and underpin the design of scaffolds that can be produced easily with sophisticated micro- and nano-scale curved features to regulate cell behavior in tissue engineering.</p>","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"9 3","pages":"681"},"PeriodicalIF":8.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/93/28/IJB-9-3-681.PMC10236331.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9950895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computational simulation-based comparative analysis of standard 3D printing and conical nozzles for pneumatic and piston-driven bioprinting.","authors":"Juan Carlos Gómez-Blanco,&nbsp;J Blas Pagador,&nbsp;Victor P Galván-Chacón,&nbsp;Luisa F Sánchez-Peralta,&nbsp;Manuel Matamoros,&nbsp;Alfonso Marcos,&nbsp;Francisco M Sánchez-Margallo","doi":"10.18063/ijb.730","DOIUrl":"https://doi.org/10.18063/ijb.730","url":null,"abstract":"<p><p>Bioprinting is an application of additive manufacturing that can deliver promising results in regenerative medicine. Hydrogels, as the most used materials in bioprinting, are experimentally analyzed to assure printability and suitability for cell culture. Besides hydrogel features, the inner geometry of the microextrusion head might have an equal impact not only on printability but also on cellular viability. In this regard, standard 3D printing nozzles have been widely studied to reduce inner pressure and get faster printings using highly viscous melted polymers. Computational fluid dynamics is a useful tool capable of simulating and predicting the hydrogel behavior when the extruder inner geometry is modified. Hence, the objective of this work is to comparatively study the performance of a standard 3D printing and conical nozzles in a microextrusion bioprinting process through computational simulation. Three bioprinting parameters, namely pressure, velocity, and shear stress, were calculated using the level-set method, considering a 22G conical tip and a 0.4 mm nozzle. Additionally, two microextrusion models, pneumatic and piston-driven, were simulated using dispensing pressure (15 kPa) and volumetric flow (10 mm³/s) as input, respectively. The results showed that the standard nozzle is suitable for bioprinting procedures. Specifically, the inner geometry of the nozzle increases the flow rate, while reducing the dispensing pressure and maintaining similar shear stress compared to the conical tip commonly used in bioprinting.</p>","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"9 4","pages":"730"},"PeriodicalIF":8.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/29/a3/IJB-9-4-730.PMC10261129.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9655822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}