Biochemical Genetics最新文献_第6页

Dihydromyricetin (DHM) Inhibits Microglial Pyroptosis and Oxidative Stress After Spinal Cord Injury by Promoting STING-Mediated Autophagy. 二氢杨梅素（DHM）通过促进sting介导的自噬抑制脊髓损伤后小胶质细胞焦亡和氧化应激。

IF 1.6 4区生物学

Biochemical Genetics Pub Date : 2025-07-29 DOI: 10.1007/s10528-025-11217-w

Ruyin Liu, Zongjin Yue, Jiaan Dong, Cheng Zhang, Chuanghao Guo, Xinli Wang

{"title":"Dihydromyricetin (DHM) Inhibits Microglial Pyroptosis and Oxidative Stress After Spinal Cord Injury by Promoting STING-Mediated Autophagy.","authors":"Ruyin Liu, Zongjin Yue, Jiaan Dong, Cheng Zhang, Chuanghao Guo, Xinli Wang","doi":"10.1007/s10528-025-11217-w","DOIUrl":"https://doi.org/10.1007/s10528-025-11217-w","url":null,"abstract":"Dihydromyricetin (DHM) inhibits the progression of neuroinflammation-related diseases. Here, we aimed to explore the effect of DHM on spinal cord injury (SCI). First, an SCI mouse model was established by external force injury and treated with DHM (150 mg/kg) by gavage for 28 days. The results showed that DHM alleviated neuronal injury and improved motor dysfunction in SCI mice. Moreover, DHM decreased the levels of pyroptosis-related proteins, such as IL-18, NLRP3, ASC, GSDMD, and Caspase-1, and upregulated the levels of oxidative stress-related proteins, such as SOD1 and HO-1, in SCI mice. Interestingly, DHM inhibited the stimulator of interferon gene (STING) protein expression in SCI mice. Next, microglia (BV-2 cells) were treated with hydrogen peroxide (H2O2) to construct a cell model and pretreated with 100 μM DHM for 24 h. The results showed that DHM promoted the expression of autophagy-related proteins (Beclin1, VPS34, CTSD, and LC3II), inhibited oxidative stress and pyroptosisin H2O2-induced BV-2 cells. 3-MA, an inhibitor of autophagy, STING overexpression vectors, and small interfering RNAs were used to reverse the effect of DHM on H2O2-induced microglia. 3-MA counteracted the protective effect of DHM on H2O2-induced BV-2 cells. Silencing STING induced autophagy and inhibited oxidative stress and pyroptosis in H2O2-induced BV-2 cells; overexpression of STING promoted the phosphorylation of PI3K and AKT. 740 Y-P, an activator of PI3K, reversed the effects of STING silencing on H2O2-induced BV-2 cells. In conclusion, DHM inhibited pyroptosis and oxidative stress by upregulating STING-mediated autophagy, thus improving motor dysfunction in SCI mice.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144740780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intersection of COVID-19 and Alzheimer's Disease: Genetic Insights and Neuropathological Consequences. COVID-19和阿尔茨海默病的交叉：遗传见解和神经病理学后果。

IF 1.6 4区生物学

Biochemical Genetics Pub Date : 2025-07-28 DOI: 10.1007/s10528-025-11208-x

Ranjith Balakrishnan, Rajasekaran Subbarayan, Rupendra Shrestha, Ankush Chauhan

{"title":"Intersection of COVID-19 and Alzheimer's Disease: Genetic Insights and Neuropathological Consequences.","authors":"Ranjith Balakrishnan, Rajasekaran Subbarayan, Rupendra Shrestha, Ankush Chauhan","doi":"10.1007/s10528-025-11208-x","DOIUrl":"https://doi.org/10.1007/s10528-025-11208-x","url":null,"abstract":"The potential link between viral infections and the onset of Alzheimer's disease (AD) has been debated for several years. The emergence of the SARS-CoV-2 pandemic has raised concerns regarding its potential role in predisposing individuals to AD or aggravating its progression. The widespread transmission of SARS-CoV-2 has introduced novel aspects to AD research, driving comprehensive investigations into the possible correlation between COVID-19 and neuropathological manifestations observed in patients with AD. This review explores the complex connection between COVID-19 and Alzheimer's disease by examining both the direct effects of SARS-CoV-2 on the brain and the indirect impacts of the infection on the overall health of individuals with Alzheimer's disease. An overview of COVID-19 is provided, followed by a detailed discussion of Alzheimer's disease, including its clinical presentation and neuropathological consequences. Moreover, our review aimed to identify key candidate genes, such as ACE2, TMPRSS2, OAS1, and APOE4, which have been implicated in COVID-19 and AD. We analyzed data from multiple genomic and transcriptomic databases to elucidate the genetic factors underlying the association between these two conditions. Furthermore, with advancements in contemporary genomic technologies, this review highlights potential genetic mutations and variations that may serve as crucial biomarkers, risk predictors, and therapeutic targets. Elucidating these molecular interactions could offer critical insights for advancing future research and formulating innovative therapeutic interventions for Alzheimer's disease within the framework of SARS-CoV-2 infection.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic Insights of Biosynthetic Potential from Actinoalloteichus caeruleus LHW52806, a Promising Actinomycete for Natural Product Discovery. 放线菌LHW52806生物合成潜力的基因组分析

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-07-24 DOI: 10.1007/s10528-025-11195-z

Hui Hong, Die Zhang, Hou-Wen Lin, Lei Li

{"title":"Genomic Insights of Biosynthetic Potential from Actinoalloteichus caeruleus LHW52806, a Promising Actinomycete for Natural Product Discovery.","authors":"Hui Hong, Die Zhang, Hou-Wen Lin, Lei Li","doi":"10.1007/s10528-025-11195-z","DOIUrl":"https://doi.org/10.1007/s10528-025-11195-z","url":null,"abstract":"Actinoalloteichus caeruleus, the type species of the rare actinomycete genus Actinoalloteichus, was found to produce various classes of bioactive compounds including bipyridine, β-carboline and cyclolipopeptides. Although the chemistry of natural products from A. caeruleus has been well studied over the past two decades, the complete genomes of this talent species along with its secondary metabolic potential was poorly reported. In this work, the complete genome sequence of Actinoalloteichus sp. LHW52806, the only producer of Marinacarboline glucuronic and Cyanogripeptides, was sequenced and the biosynthetic potential was investigated by a genome mining approach. Genomic analyses indicate a circular chromosome consisting of 6,184,314 bp with a G + C content of 72.27%. The dDDH and ANI values based on genome sequences between strain LHW52806 and the type strain, Actinoalloteichus caeruleus NRRL B-2194T, were 93.1% and 99.2%, respectively, confirmed the species assignment. Combined analysis using antiSMASH and PRISM identified 27 biosynthetic gene clusters (BGCs) responsible for secondary metabolite. Among these, only 10 BGCs exhibited high similarities to known clusters in the MIBIG database. The remaining clusters are predicted to synthesize novel natural products, encompassing nonribosomal peptides (NRPs), polyketides (PKs), lassopeptides, lantibiotics, and guanidinotides, suggesting substantial biosynthetic potential. The candidate BGC for β-carboline alkaloids was identified in the genome and a biosynthetic pathway of marinacarboline glucuronic was proposed. Guided by the bioinformatic analysis on the BGCs, a series of polycyclic tetramate macrolactams (PTMs) was found by LC-MS in the fermentation extracts of A. caeruleus LHW52806, with 30,31-dihydrofrontalamide A (DHFA) isolated and identified. These findings advance comprehension of secondary metabolite biosynthesis in A. caeruleus, while simultaneously establishing a foundation for novel natural products discovery.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Value Analysis of Hsa_circ_0086004 and Hsa-miR-665 in the Diagnosis of Fractures and Promotion of Fracture Healing in Osteoporosis Patients. Hsa_circ_0086004与Hsa-miR-665在骨质疏松症患者骨折诊断及促进骨折愈合中的价值分析

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-07-24 DOI: 10.1007/s10528-025-11206-z

Rui Zhu, Tao Jiang

{"title":"The Value Analysis of Hsa_circ_0086004 and Hsa-miR-665 in the Diagnosis of Fractures and Promotion of Fracture Healing in Osteoporosis Patients.","authors":"Rui Zhu, Tao Jiang","doi":"10.1007/s10528-025-11206-z","DOIUrl":"https://doi.org/10.1007/s10528-025-11206-z","url":null,"abstract":"Fractures are common in osteoporosis (OP) patients. To discover new biomarkers for reducing the incidence of fractures by taking preventive measures. Hsa_circ_0086004 and hsa-miR-665 were screened using the GEO and starBase databases. Their diagnostic and prognostic value in osteoporotic fractures was evaluated by constructing ROC curves. An osteogenic differentiation medium (OM) was used to induce osteogenic differentiation of human mesenchymal stem cells (hMSCs). Transfected hsa_circ_0086004 plasmid to detect its regulation on hsa-miR-665 and effects on the function of osteoblasts and osteogenic differentiation, and the hsa-miR-665 role in which was determined by transfecting its inhibitor and mimic. Hsa_circ_0086004 was deficient in the serum of OP patients, and its level was further decreased with the occurrence of fractures. Hsa-miR-665 was opposite. Overexpression of hsa_circ_0086004 and inhibition of hsa-miR-665 promoted the growth of osteoblasts and facilitated the osteogenic differentiation of hMSCs. Transfection of hsa_circ_0086004 plasmid significantly decreased the cellular level of hsa-miR-665. The combination of hsa_circ_0086004 and hsa-miR-665 could predict the occurrence and healing of fracture in OP patients, with AUC of 0.907 and 0.902, respectively. Hsa_circ_0086004 may promote fracture healing in OP patients by inhibiting hsa-miR-665, and they are potential biomarkers of OP.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circ-Hipk3 in Adipose Derived Stem Cells Exosome Alleviates Myocardial Infarction Induced Myocardial Damage by Regulation miR-138-5p/Sirt1 Axis Mediated Autophagy. 脂肪源性干细胞外泌体Circ-Hipk3通过调节miR-138-5p/Sirt1轴介导的自噬减轻心肌梗死引起的心肌损伤

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-07-23 DOI: 10.1007/s10528-025-11173-5

Guoxun Yang, Danpeng Nong, Xiao Tang, Ling Tan, Peng Yang, Haien Ning

{"title":"Circ-Hipk3 in Adipose Derived Stem Cells Exosome Alleviates Myocardial Infarction Induced Myocardial Damage by Regulation miR-138-5p/Sirt1 Axis Mediated Autophagy.","authors":"Guoxun Yang, Danpeng Nong, Xiao Tang, Ling Tan, Peng Yang, Haien Ning","doi":"10.1007/s10528-025-11173-5","DOIUrl":"https://doi.org/10.1007/s10528-025-11173-5","url":null,"abstract":"Myocardial infarction (MI) is a major health problem and is the leading cause of death worldwide. Accumulation studies confirm that exosomes derived from stem cells have been shown to effectively repair MI injury-induced cardiomyocyte damage. However, the cardioprotective benefits of adipose tissue-derived mesenchymal stem cell (ADSC)-Exos remain unclear. This study aimed to investigate the protective effects of exosomes from ADSC on the hearts of MI-treated mice and to explore the underlying mechanisms. Cellular and molecular mechanisms were investigated using cultured ADSCs. On C57BL/6 J mice, we performed myocardial MI model. HMEC-1 was used to identify the regulatory mechanism. ELISA was used for inflammatory factor expression detection. Luciferase report analysis detection showed the relationship among miR-138-5p, Sirt1 and circ-Hipk3. ADSCs exosome treatment significantly alleviates MI induced myocardial damage by promotion autophagy and inhibits inflammatory response. Circ-Hipk3 play an important role in ADSCs exosome mediated protective effect on MI induced myocardial damage. Luciferase report analysis confirmed that miR-138-5p and SIRT1 were at circ-Hipk3 downstream. Downregulation SIRT1 or upregulation miR-138-5p reversed the regulation effect of circ-Hipk3 on autophagy and inflammatory factor expression. Overexpression circ-Hipk3 increased the protective effect of ADSC-Exo on MI induced myocardial damage. In summary, these findings show that circ-Hipk3 from ADSC-Exos can alleviate cardiac injury in MI-treated mice via the miR-138-5p/Sirt1 axis mediated autophagy signaling pathway. ADSC-Exos containing circ-Hipk3 has a promising therapeutic potential in MI induced cardiac injury.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Prognostic-Related Genes in Acute Myeloid Leukemia: A Study Based on TCGA Data Analysis. 基于TCGA数据分析的急性髓系白血病预后相关基因鉴定

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-07-23 DOI: 10.1007/s10528-025-11193-1

Fatemeh Sadat Shafiei, Saeid Abroun, Sadaf Vahdat, Ali Arash Anoushirvani, Mohammad Rafiee

{"title":"Identification of Prognostic-Related Genes in Acute Myeloid Leukemia: A Study Based on TCGA Data Analysis.","authors":"Fatemeh Sadat Shafiei, Saeid Abroun, Sadaf Vahdat, Ali Arash Anoushirvani, Mohammad Rafiee","doi":"10.1007/s10528-025-11193-1","DOIUrl":"https://doi.org/10.1007/s10528-025-11193-1","url":null,"abstract":"Acute myeloid leukemia (AML) is the most common type of leukemia in adults, primarily caused by multiple gene mutations and abnormal gene expression. Molecular heterogeneity among AML patients can lead to the variation of treatment outcomes, and the prognostic significance of genetic disruptions is crucial for treatment decisions. Therefore, in this study, we intended to identify novel potential prognosis-related genes in AML patients. This study comprehensively assessed transcriptomic data of primary AML patients from TARGET and BEAT-AML cohorts from the TCGA database. The common differentially expressed mRNAs (DEmRNAs) of the study groups were determined and screened to identify genes that indicated a correlation between their expression levels and the overall survival (OS) of AML patients. Moreover, RT-PCR was used to compare the expression of the identified prognosis-related genes between AML patients and non-leukemic groups to confirm the obtained bioinformatics data. The analysis resulted in the identification of 39 common significant DEmRNAs in both cohorts. Moreover, among the identified common genes, the expression levels of two genes, MME and RBM11, significantly correlated with the OS of AML patients; it was revealed that there was a significant negative correlation between a higher survival rate in AML patients and the lower expression of MME (log-rank P = 1.3*10-7 and Hazard Ratio (HR = 1.38 (1.22-1.56)) and RBM11 (log-rank P = 0.016 and HR = 1.25 (1.04-1.5)). Furthermore, RT-PCR data confirmed the expected differential expression of identified genes between patient and control samples. In conclusion, our investigation resulted in the identification of two potential prognosis-related genes that can be used in further prognostic evaluation studies.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Liver Kinase B1 Protects Against Hypoxia-Induced Pulmonary Arterial Endothelial Cell Dysfunction via the AMP-Activated Protein Kinase Pathway. 更正：肝激酶B1通过amp激活的蛋白激酶途径保护缺氧诱导的肺动脉内皮细胞功能障碍。

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-07-21 DOI: 10.1007/s10528-025-11196-y

Bingchang Hei, Anzhe Zhang, Meiming Yang, Yunfei Jiang, Zhanjiang Guan

引用次数: 0

Phylogenetic Characteristics of a Newly Sequenced Mitochondrial Genome of Monodactylus sebae (Eupercaria, Monodactylidae). sebae单爪虫线粒体基因组新测序的系统发育特征。

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-07-18 DOI: 10.1007/s10528-025-11185-1

Xiao-Die Chen, Cheng-He Sun, Chang-Hu Lu

{"title":"Phylogenetic Characteristics of a Newly Sequenced Mitochondrial Genome of Monodactylus sebae (Eupercaria, Monodactylidae).","authors":"Xiao-Die Chen, Cheng-He Sun, Chang-Hu Lu","doi":"10.1007/s10528-025-11185-1","DOIUrl":"https://doi.org/10.1007/s10528-025-11185-1","url":null,"abstract":"The African moony (Monodactylus sebae) occupies a unique ecological niche and possesses significant aesthetic value, playing a crucial role in aquatic ecosystems. To elucidate the phylogenetic position of the African moony within the family Monodactylidae, this study employed high-throughput sequencing technology to obtain the mitochondrial genome of the African moony. A phylogenetic tree was subsequently constructed based on 13 protein-coding genes. The mitochondrial DNA of the African moony spans a total length of 16,544 base pairs, encoding 22 transfer RNAs, 13 genes associated with protein synthesis, and 2 ribosomal RNAs, with an additional D-loop region. The genome exhibits a pronounced bias towards adenine-thymine (AT) nucleotides, particularly in the D-loop region, where the AT content reaches as high as 65.5%. Utilizing Bayesian inference and maximum likelihood methods to reconstruct the phylogenetic tree, the analysis revealed that the African moony (M. sebae) and the Silver moony (Monodactylus argenteus) form a monophyletic clade, indicating a close phylogenetic relationship. Moreover, the phylogenetic tree unveiled an early rapid radiation event in the evolution of Eupercaria, offering novel molecular insights into the mechanisms underlying fish biodiversity. This study not only enriches the mitochondrial genome database for fish species but also lays a vital foundation for the follow-up study on the adaptive evolution and conservation biology of the African moony.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association Between Ferroptosis and Acute Kidney Disease (AKD): Unveiling the Expression of Genes Related to Iron Regulatory Metabolism. 铁下垂与急性肾病（AKD）之间的关系：揭示铁调节代谢相关基因的表达

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-07-18 DOI: 10.1007/s10528-025-11188-y

Nayab Khalid, Zertashia Akram, Nighat Hayat, Ambar Siddique, Asma Gul, Hifza Wajid

{"title":"Association Between Ferroptosis and Acute Kidney Disease (AKD): Unveiling the Expression of Genes Related to Iron Regulatory Metabolism.","authors":"Nayab Khalid, Zertashia Akram, Nighat Hayat, Ambar Siddique, Asma Gul, Hifza Wajid","doi":"10.1007/s10528-025-11188-y","DOIUrl":"https://doi.org/10.1007/s10528-025-11188-y","url":null,"abstract":"Acute kidney disease (AKD), a condition between AKI and CKD, was categorized as a separate entity by KDIGO in 2012, although it remains debatable. The present study investigates mechanism underlying AKD condition with ferroptosis, lipid peroxidation and related genes. Blood samples were collected from AKD patients (n = 100) along with age and gender matched control. Expression analysis of GPX4, EPO, TF, HP, FTH1, NCOA4 genes was done using RT-PCR. Peroxidase activity, lipid peroxidation, serum ferritin and total protein were measured. Relative expressions of GPX4, EPO and TF were downregulated (P < 0.001) while FTH1, HP and NCOA4 expression were upregulated (P < 0.001) in AKD group compared to controls. Similar trend was observed between male AKD group versus male control (P < 0.001) and female AKD group versus female controls (P < 0.001). Expression deregulation of selected genes was detected in both age groups (≤ 40 year & > 40 year age) of AKD patents compared to their respective controls. AKD group showed significant (P < 0.001) increase in serum ferritin levels compared to respective control. Low activity of POD and elevated levels of lipid peroxidation was observed in male (P < 0.001) and female (P < 0.01) AKD patients compared to controls. Present study suggested a positive contribution of lipid-mediated ferroptosis in progression of AKD. Furthermore, expression deregulation of genes underlying iron metabolism along with depleted expression of antioxidant gene and low peroxidase activity postulated iron overload as possible candidate in etiology of AKD condition.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

VHL Suppresses Angiogenesis Through HIF-1a-Mediated Ang/Tie2/AMPK/VEGF Signaling Pathway in Tie-2 Expressed Macrophages (TEMs). VHL通过hif -1a介导的Tie-2表达巨噬细胞中Ang/Tie2/AMPK/VEGF信号通路抑制血管生成

IF 2.1 4区生物学

Biochemical Genetics Pub Date : 2025-07-16 DOI: 10.1007/s10528-025-11175-3

Min-Cheng Zou, Yu-Hao Yang, Yun-Peng Mao, Ya Liu, Hui-Bing Gao, Wen-Dong Liu, Jia Liu, Fu-Yong Zhang

{"title":"VHL Suppresses Angiogenesis Through HIF-1a-Mediated Ang/Tie2/AMPK/VEGF Signaling Pathway in Tie-2 Expressed Macrophages (TEMs).","authors":"Min-Cheng Zou, Yu-Hao Yang, Yun-Peng Mao, Ya Liu, Hui-Bing Gao, Wen-Dong Liu, Jia Liu, Fu-Yong Zhang","doi":"10.1007/s10528-025-11175-3","DOIUrl":"https://doi.org/10.1007/s10528-025-11175-3","url":null,"abstract":"The cartilage of the growth plate is crucial for the longitudinal growth of long bones but is highly susceptible to injury due to its avascular nature. Growth plate injuries frequently result in the formation of a bone bridge, leading to limb length discrepancies and angular deformities. Angiogenesis is a critical factor in the repair process, as new blood vessels deliver oxygen, nutrients, and cellular components essential for bone regeneration. Tie2-expressing macrophages (TEMs) play a pivotal role in promoting angiogenesis in tumors and remodeled tissues; however, their precise function and regulatory mechanisms in epiphyseal plate injury repair remain unclear. This study investigates the role of the VHL/HIF-1α/Tie2/AMPK/Autophagy axis in TEM-mediated angiogenesis. Our findings identify VHL as a key regulator of TEM-driven angiogenesis, where VHL overexpression suppresses, and VHL silencing enhances the pro-angiogenic potential of TEMs. Mechanistically, VHL downregulates HIF-1α, reducing Tie2 surface expression, which in turn modulates AMPK-mediated autophagy. This pathway influences VEGF secretion, thereby promoting endothelial cell proliferation, migration, survival, and tube formation. These findings uncover a novel regulatory mechanism governing TEM-mediated angiogenesis and offer insights into potential therapeutic strategies to enhance vascularization, improve growth plate injury repair, and mitigate long-term orthopedic complications.","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0