Einstein-Sao Paulo最新文献

{"title":"Prevalence of autoimmune diseases in patients with type 1 diabetes: a scoping review.","authors":"Carlos Antonio Negrato, Rebecca Zerbinatti Pereira, Livia Domingos de Moraes Pimentel Porto, Ylana Walleska Santos Santana, Aline Kimmy Ikemoto Sato, Vitor Casoto de Melo, Miguel Luz Vilela Engel Vieira, Marilia de Brito Gomes","doi":"10.31744/einstein_journal/2025RW1222","DOIUrl":"https://doi.org/10.31744/einstein_journal/2025RW1222","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the prevalence of autoimmune diseases in patients with type 1 diabetes mellitus.</p><p><strong>Methods: </strong>This scoping review was conducted following the Joanna Briggs Institute guidelines and the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Reviews (PRISMA-ScR) tool to ensure methodological rigor. We systematically searched PubMed, Embase, Scopus, Lilacs, and Web of Science databases to identify relevant literature published between 2018 and 2023.</p><p><strong>Results: </strong>Twenty-four studies were included, mostly single-center studies from six continents, with varying study designs: 16 cross-sectional, seven retrospective, and one prospective cohort. The most prevalent autoimmune diseases found among patients with type 1 diabetes mellitus enrolled in these studies were autoimmune thyroiditis (5.5-41.2%), celiac disease (0.45-24.8%), rheumatoid arthritis (0.4-5.1%), and primary adrenal insufficiency (0.6-2.6%).</p><p><strong>Conclusion: </strong>Autoimmune thyroiditis and celiac disease were the most prevalent autoimmune diseases in patients with type 1 diabetes mellitus. As the complexity of managing type 1 diabetes mellitus increases in the presence of multiple autoimmune comorbidities, further studies are required to elucidate the relationship between type 1 diabetes mellitus and different autoimmune pathologies. A deeper understanding of these associations will guide the development of public health policies, screening strategies, and educational initiatives tailored to the specific needs of this population.</p>","PeriodicalId":47359,"journal":{"name":"Einstein-Sao Paulo","volume":"23 ","pages":"eRW1222"},"PeriodicalIF":1.1,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061443/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144034879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Socioeconomic inequities in prostate cancer care: private versus public treatment settings pose a significant impact on overall survival.","authors":"Amanda Caroline de Souza Costa, Fernando Korkes, Jose Augusto Rinck Junior, Frederico Timóteo Silva Cunha, Luciana Holtz de Camargo Barros, Stênio de Cássio Zequi, Maria Nirvana da Cruz Formiga","doi":"10.31744/einstein_journal/2025AO0851","DOIUrl":"https://doi.org/10.31744/einstein_journal/2025AO0851","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the impact of assistance from public and private health systems on the overall survival of patients with metastatic prostate cancer.</p><p><strong>Methods: </strong>A total of 213 patients with metastatic prostate cancer treated at the A.C. Camargo Cancer Center, either in the Unified Health System or a private system, from January 2014 to December 2018 were analyzed. Multivariate analysis of overall survival was performed to adjust for the type of health assistance and other clinical prognostic factors.</p><p><strong>Results: </strong>Of 213 included patients, 147 (69%) were from the private system and 66 (31%) were from the Unified Health System. There was a significant difference in the median age at diagnosis between the systems (63.4 years for patients in the private system versus 67.2 years for patients in the Unified Health System, p=0.027). No significant differences in performance status were observed between the groups (p=0.695). Patients treated in the public system had access to fewer treatment lines (2.59 lines in the public system versus 3.04 lines in the private system, p=0.024). Our data revealed a longer median survival for patients with private health care (115 months for patients with private health care versus 78 months for patients in the Unified Health System, p=0.009). Multivariate analysis revealed that patients in the public system had a 66% higher risk of death than those in the private system.</p><p><strong>Conclusion: </strong>Our data demonstrate that patients in the Unified Health System have access to fewer lines of therapy for metastatic prostate cancer, resulting in shorter median survival than patients treated in the private system.</p>","PeriodicalId":47359,"journal":{"name":"Einstein-Sao Paulo","volume":"23 ","pages":"eAO0851"},"PeriodicalIF":1.1,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061437/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144054262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Psychological impact of gestational trophoblastic disease: a cross-sectional study.","authors":"Natália Giovanelli Gaspar, Adrielle Carolina Ferreira Silva, Cristina Laguna Benetti-Pinto, Daniela Angerame Yela","doi":"10.31744/einstein_journal/2025AO1014","DOIUrl":"https://doi.org/10.31744/einstein_journal/2025AO1014","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the psychological repercussions of gestational trophoblastic disease.</p><p><strong>Methods: </strong>A cross-sectional study including 100 women (50 with gestational trophoblastic disease and 50 without) was conducted between September 2020 and October 2021. Socio-demographic characteristics, quality of life, depression, and anxiety were evaluated and follow-up was performed at a referral center.</p><p><strong>Results: </strong>Women with gestational trophoblastic disease had a mean age and body mass index of 28.8±6.4 years and 24.7±5.0kg/m2, respectively; the corresponding values for the Control Group were 30.1±6.9 years (p=0.27) and 28.9±4.6kg/m2 (p<0.001). The Gestational Trophoblastic Disease Group presented a better general health status (p=0.04) than the Control Group. Among women with gestational trophoblastic disease, 62% and 46% had anxiety and depression, respectively; the corresponding percentages for women without gestational trophoblastic disease were 52% and 24% (p=0.64 and 0.08, respectively).</p><p><strong>Conclusion: </strong>Multidisciplinary follow-up at a referral center may improve the mental health and quality of life scores of women with gestational trophoblastic disease.</p>","PeriodicalId":47359,"journal":{"name":"Einstein-Sao Paulo","volume":"23 ","pages":"eAO1014"},"PeriodicalIF":1.1,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061436/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144004422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modified palatal flap via soft palate for skull base reconstruction.","authors":"Rogerio Pezato, Aldo Cassol Stamm, Andrea Santos Dumont Costacurta, Carlos Henrique Amaro Bravo Baptista, Reginaldo Raimundo Fujita, Camila Soares Dassi, Richard Louis Voegels, Andrew Thamboo, Miguel Soares Tepedino","doi":"10.31744/einstein_journal/2025AO1247","DOIUrl":"https://doi.org/10.31744/einstein_journal/2025AO1247","url":null,"abstract":"<p><strong>Objective: </strong>Given the importance of vascularized flaps, this study presents a new technique for reconstructing skull defects using a pedicled palatal flap via the soft palate.</p><p><strong>Methods: </strong>Five preserved cadaveric specimens were used to demonstrate the sequential steps involved in harvesting the palatal flap. In addition, 20 normal paranasal sinus computed tomography scans were analyzed to determine potential measurements of the flap (area, perimeter, greatest anteroposterior distance, and greatest transverse distance).</p><p><strong>Results: </strong>The average flap area in females and males was 11.8cm2 and 12.7cm2, respectively. The average perimeter in females and males was 13.5cm and 14.0cm, respectively. In all cases, the flap reached the infratemporal fossa and lower clivus.</p><p><strong>Conclusion: </strong>The palatal flap via the soft palate described in this study proved to be a metrically viable alternative in cadaveric and tomographic studies for use in endonasal surgeries, as well as for skull base and adjacent regions.</p>","PeriodicalId":47359,"journal":{"name":"Einstein-Sao Paulo","volume":"23 ","pages":"eAO1247"},"PeriodicalIF":1.1,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061439/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144015906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dose-response relationships in Y90 resin microsphere radioembolization for patients with hepatocellular carcinoma: insights from a Brazilian cohort.","authors":"Marcela Juliano Silva Cunha, Francisco Leonardo Galastri, Felipe Nasser, Breno Boueri Affonso, Leonardo Guedes Moreira Valle, Priscila Mina Falsarella, Bruno Pagnin Schmid, Lilian Yuri Itaya Yamaga, Giovanna Sawaya Torre, Rodrigo Gobbo Garcia, Nelson Wolosker","doi":"10.31744/einstein_journal/2025AO1287","DOIUrl":"https://doi.org/10.31744/einstein_journal/2025AO1287","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the relationship between the absorbed dose and radiological response in patients with hepatocellular carcinoma undergoing resin microsphere radioembolization to enhance treatment precision and potentially adjust protocols for better outcomes.</p><p><strong>Methods: </strong>This single-center retrospective study was conducted at a quaternary care center in Brazil and comprised 27 patients with 58 target lesions treated between November 2014 and April 2023. The patients' dosimetric profiles were analyzed using advanced imaging software to assess post-treatment clinical and radiological responses. This study explored the correlation between the absorbed doses and tumor objective responses using statistical analyses to confirm the findings.</p><p><strong>Results: </strong>Higher absorbed doses were significantly associated with improved objective responses in the target lesions. The analysis demonstrated that achieving higher dosimetric thresholds correlated with better clinical outcomes, suggesting that a dose-response relationship is essential to optimize treatment protocols.</p><p><strong>Conclusion: </strong>These findings emphasize the importance of personalized dosimetry in managing hepatocellular carcinoma using resin microsphere radioembolization. By quantifying the absorbed dose and understanding its impact on tumor response, treatment strategies can be tailored to enhance efficacy and potentially shift palliative treatments toward curative outcomes. This study provides a crucial foundation for dosimetric adjustment and highlights the need for further research in this area, particularly in Brazil.</p>","PeriodicalId":47359,"journal":{"name":"Einstein-Sao Paulo","volume":"23 ","pages":"eAO1287"},"PeriodicalIF":1.1,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061438/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143991431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Moebius Syndrome associated with syringomyelia: a case report.","authors":"Fernanda Kimie Yamamoto, Fernanda Marques de Deus, Renata de Araújo Monteiro Yoshida, Erasmo Barbante Casella, Romy Schmidt Brock Zacharias","doi":"10.31744/einstein_journal/2025RC0876","DOIUrl":"https://doi.org/10.31744/einstein_journal/2025RC0876","url":null,"abstract":"<p><strong>Background: </strong>Moebius Syndrome is a genetic condition that results in inability for facial mimicry due to palsy of cranial nerves VI and VII. Syringomyelia is characterized by a dilation of the central canal in the spinal cord, and is generally asymptomatic. Both disorders are diagnosed using imaging tests. A newborn with no facial movements upon physical examination was admitted to the delivery room of our hospital. A specialist confirmed the condition to be Moebius Syndrome. Further investigation using magnetic resonance imaging indicated that syringomyelia was associated with Moebius Syndrome. Given that syringomyelia is a silent myelopathy with a possible impact on the future health of the patient, careful analysis is recommended when diagnosing Moebius Syndrome. Few similar cases have been reported to date. Further studies are warranted to determine the actual association between these two conditions. Since syringomyelia diagnoses are often made when testing for other conditions, as in this study, neurological examinations of the brain should be expanded to include the spinal cord, in order to verify the presence of coexisting disorders.</p>","PeriodicalId":47359,"journal":{"name":"Einstein-Sao Paulo","volume":"23 ","pages":"eRC0876"},"PeriodicalIF":1.1,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061435/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144052074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chryseobacterium indologenes sepsis in a pediatric patient.","authors":"Felipe Krakauer, Raquel Pissiguelli, Marina Moranduzzo, Bruna Marques Tofaneto, Sophia Bastos Correa da Costa, Maryleen Brigitte Muñoz Guzman, Gabriel Frizzo Ramos, Saulo Brasil do Couto, Rafael Yanes Rodrigues da Silva, Luís Felipe Batista Hiar, Luisa Zagne Braz","doi":"10.31744/einstein_journal/2025RC1200","DOIUrl":"https://doi.org/10.31744/einstein_journal/2025RC1200","url":null,"abstract":"<p><strong>Background: </strong>Chryseobacterium indologenes is a Gram-negative aerobic bacillus commonly found in nosocomial environments, particularly in patients with prolonged hospital stays or those requiring long-term invasive devices. It primarily affects elderly and immunocompromised individuals. This microorganism is associated with multidrug resistance, which is a crucial factor in treatment decisions. Here, we report a case of C. indologenes infection in an infant following the ingestion of milk formula diluted with untreated water.</p>","PeriodicalId":47359,"journal":{"name":"Einstein-Sao Paulo","volume":"23 ","pages":"eRC1200"},"PeriodicalIF":1.1,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061444/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tenosynovitis caused by Trichosporon asahii in an immunocompetent patient.","authors":"Lais Lopes Pires, Mayara Gabriele Toledo, Hugo Rodrigues Alves, Isadora Cambruzzi, Guilherme Brezenski Rodrigues, Andrea D'avila Freitas, Natalia Chilinque Zambao da Silva","doi":"10.31744/einstein_journal/2025RC1095","DOIUrl":"https://doi.org/10.31744/einstein_journal/2025RC1095","url":null,"abstract":"<p><strong>Background: </strong>Fungal infections have become a public health problem owingdute to their progressive increase in recent decades and high morbidity rates. Fungal bone and joint infections result from direct inoculation, contiguous infection spread, or hematogenous seeding of organisms. Trichosporon spp. are yeast-like basidiomycetes, with Trichosporon asahii being the most common pathogenic species. This article describes a rare case of tenosynovitis caused by Trichosporon asahii in an immunocompetent patient. Treatment with voriconazole resulted in an excellent clinical response.</p>","PeriodicalId":47359,"journal":{"name":"Einstein-Sao Paulo","volume":"23 ","pages":"eRC1095"},"PeriodicalIF":1.1,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061440/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A cross-sectional study on the expression of fibrinolytic system components and metalloproteinase-9 in women with early-stage and metastases breast cancer in Tucumán, Argentina.","authors":"Mariano Nicolás Áleman, María Constanza Luciardi, Rosa Silvina Guber, Analía Graciela Soria","doi":"10.31744/einstein_journal/2025AO1237","DOIUrl":"https://doi.org/10.31744/einstein_journal/2025AO1237","url":null,"abstract":"<p><strong>Background: </strong>In this study, standard coagulation, t-PA, PAI-1, DD, and MMP-9 tests were performed in a specific population of patient with early and metastatic breast cancer in Tucumán, Argentina. Only PAI-1 and MMP-9 levels increased in patients with early-stage breast cancer, suggesting that they may have evolved aggressively.</p><p><strong>Background: </strong>■ No clinically significant differences were observed in platelet counts or global coagulation test results.</p><p><strong>Background: </strong>■ t-PA values decreased in patients with metastases.</p><p><strong>Background: </strong>■ PAI-1, DD, and MMP-9 levels increased in patients with metastases.</p><p><strong>Background: </strong>■ High levels of PAI-1 and MMP-9 in the early stages could indicate an aggressive course in patients.</p><p><strong>Objective: </strong>The hemostatic and matrix metalloproteinase (MMP) systems are known to be altered in patients with breast cancer. This cross-sectional study aimed to explore the fibrinolysis components values and MMP-9 levels in a specific population of patients with early breast cancer and metastatic breast cancer in Tucumán, Argentina.</p><p><strong>Methods: </strong>This cross-sectional study included 63 patients without pathology or with benign breast pathology (GA Group), 48 patients with newly diagnosed primary breast cancer (GB Group), and 65 patients with metastatic breast cancer (GC Group). Levels of standard coagulation tests, tissue plasminogen activator (t-PA), plasminogen activator inhibitor-1 (PAI-1), D-dimer (DD), and matrix metalloproteinases-9 (MMP-9) were measured in all groups.</p><p><strong>Results: </strong>PAI-1 and MMP-9 levels were higher in patient with early breast cancer than those with benign disease. However, the GC Group showed significantly higher PAI-1, DD, and MMP-9 levels than the GA Group. Furthermore, the t-PA concentrations in the GC Group were lower than those in the Control Group.</p><p><strong>Conclusion: </strong>Our findings suggest that higher levels of PAI-1 and MMP-9 in patients with early-stage breast cancer may be associated with a subgroup of patients with more aggressive disease progression. In addition, significant alterations in t-PA, PAI-1, DD, and MMP-9 concentrations were observed in advanced stages of the disease. However, basic laboratory tests, such as global coagulation tests, do not effectively differentiate stages in breast cancer. Therefore, further study is needed to explore these findings in greater detail.</p>","PeriodicalId":47359,"journal":{"name":"Einstein-Sao Paulo","volume":"23 ","pages":"eAO1237"},"PeriodicalIF":1.1,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061442/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144024795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of miRNA-mRNA interaction prediction tools based on experimental head and neck cancer data.","authors":"Bárbara Dos Santos Dias, Larissa Figueiredo Alves Diniz, Lucca D'Arco Corrêa, Rafael Pereira de Souza, Leticia Torres Ferreira, Denise da Cunha Pasqualin, Rafael de Cicco, Eloiza Helena Tajara da Silva, Patricia Severino","doi":"10.31744/einstein_journal/2025AO1372","DOIUrl":"https://doi.org/10.31744/einstein_journal/2025AO1372","url":null,"abstract":"<p><strong>Background: </strong>We evaluated the performance of TargetScan, miRDB, and miRWalk for predicting miRNA-mRNA interactions in HNSCC. Based on clinical tumor and cancer-free tissue data, miRWalk emerged as the most comprehensive tool. Validation using NanoString technology and MiRTarBase confirmed key predictions, highlighting the important roles of the PI3K-Akt and Wnt pathways. This study underscores the importance of integrating bioinformatics and experimental data to better understand HNSCC.</p><p><strong>Background: </strong>■ miRWalk had the highest predicted interactions and validated miRNA networks in HNSCC.</p><p><strong>Background: </strong>■ Around 3.3% of interactions overlapped across tools, emphasizing the need for multitool approaches.</p><p><strong>Background: </strong>■ Dysregulated genes and miRNAs were tied to cancerdriving PI3K-Akt and Wnt pathways.</p><p><strong>Background: </strong>■ The validated approach highlights the importance of integrating computational and molecular data.</p><p><strong>Objective: </strong>Head and neck squamous cell carcinoma (HNSCC) has a poor prognosis largely due to late diagnosis and a lack of reliable biomarkers. MicroRNAs (miRNAs), small non-coding RNAs that regulate gene expression, are promising biomarkers for HNSCC. This study evaluated miRNA-mRNA interactions in HNSCC using conventional computational tools and validated the results using molecular data.</p><p><strong>Methods: </strong>We compared three miRNA-mRNA interaction prediction tools, TargetScan, miRDB, and miRWalk, using differentially expressed miRNAs and mRNAs from HNSCC and cancer-free tissues. NanoString nCounter was used to measure miRNA and mRNA expression and the miRTarBase database was used to validate the predicted miRNA-mRNA interactions.</p><p><strong>Results: </strong>TargetScan and miRWalk provide a comprehensive overview of potential interactions, whereas miRDB provides functional insights. Our results identified 77 and 154 differentially expressed miRNAs and mRNAs in HNSCC, respectively. miRWalk predicted the highest number of miRNA-mRNA interactions, followed by miRDB and TargetScan. Only 3.3% of interactions were common among the tools. The MiRTarBase analysis confirmed a small subset of the predictions. Biological pathway analysis highlighted the dysregulation of PI3K-Akt and Wnt signaling; miRWalk was the best for elucidating how miRNAs modulate target mRNAs in these key pathways during HNSCC progression.</p><p><strong>Conclusion: </strong>miRWalk emerged as the most robust tool for predicting miRNA-mRNA interactions. Our findings highlight the importance of integrating bioinformatics predictions with experimental data to better understand the regulatory networks in HNSCC and identify potential biomarkers for diagnosis and therapy.</p>","PeriodicalId":47359,"journal":{"name":"Einstein-Sao Paulo","volume":"23 ","pages":"eAO1372"},"PeriodicalIF":1.1,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061445/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144015902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}