Lancet Microbe最新文献

{"title":"Global diversity and evolution of Salmonella enterica serovar Panama: a genomic epidemiology study","authors":"Caisey V Pulford PhD ,&nbsp;Blanca M Perez-Sepulveda PhD ,&nbsp;Danielle J Ingle PhD ,&nbsp;Rebecca J Bengtsson PhD ,&nbsp;Rebecca J Bennett PhD ,&nbsp;Ella V Rodwell PhD ,&nbsp;Maria Pardos de la Gandara MD ,&nbsp;Charlotte E Chong PhD ,&nbsp;P Malaka De Silva PhD ,&nbsp;Magali Ravel BTS ,&nbsp;Véronique Guibert BTS ,&nbsp;Elisabeth Njamkepo PhD ,&nbsp;Prof Neil Hall PhD ,&nbsp;Marie A Chattaway PhD ,&nbsp;Prof Benjamin P Howden PhD ,&nbsp;Prof Deborah A Williamson PhD ,&nbsp;Prof Jay C D Hinton PhD ,&nbsp;Prof François-Xavier Weill PhD ,&nbsp;Prof Kate S Baker PhD","doi":"10.1016/j.lanmic.2025.101150","DOIUrl":"10.1016/j.lanmic.2025.101150","url":null,"abstract":"<div><h3>Background</h3><div>Non-typhoidal <em>Salmonella</em> is a globally important bacterial pathogen, typically associated with foodborne gastrointestinal infection. Some non-typhoidal <em>Salmonella</em> serovars can also colonise typically sterile sites in people to cause invasive non-typhoidal <em>Salmonella</em> disease. <em>Salmonella enterica</em> serovar Panama is responsible for a substantial number of cases of human bloodstream infection, but despite its global dissemination, numerous outbreaks, and a reported association with invasive non-typhoidal <em>Salmonella</em> disease, <em>S enterica</em> serovar Panama (<em>S</em> Panama) is understudied. We aimed to describe the genomic epidemiology and evolutionary history of <em>S</em> Panama to provide a vital baseline of understanding for this globally important serovar.</div></div><div><h3>Methods</h3><div>In this genomic epidemiology study, we analysed <em>S</em> Panama genomes derived from historical collections, national surveillance datasets, and publicly available epidemiological and whole-genome sequencing data which span the years 1931–2019. Maximum likelihood and Bayesian phylodynamic approaches were used to investigate population structure and evolutionary history and to infer geotemporal dissemination. A combination of different bioinformatic approaches with short-read and long-read data were used to characterise geographical and clade-specific trends in antimicrobial resistance (AMR) and genetic markers for invasiveness.</div></div><div><h3>Findings</h3><div>We analysed 836 <em>S</em> Panama genomes, of which 559 (67%) were sequenced as part of this study. The collection represents all inhabited continents and includes isolates collected between 1931 and 2019. We identified the presence of four geographically linked <em>S</em> Panama clades (C1 [ie, the Latin America and the Caribbean clade; n=338], C2 [ie, the European clade; n=124], C3 [ie, the Martinique clade; n=131], and C4 [ie, the Asia and Oceania clade; n=104]) and regional trends in AMR profiles. Most isolates (715 [86%] of 836) were pan-susceptible to antibiotics and belonged to clades circulating in Latin America and the Caribbean (64%, n=458). Most antibiotic-resistant isolates in our collection (113 [93%] of 121) fell within clades C4 (ie, the Asia and Oceania clade) and C2 (ie, the European clade), the latter of which had the highest invasiveness index values based on the conservation of 196 extraintestinal predictor genes.</div></div><div><h3>Interpretation</h3><div>This first large-scale phylogenetic analysis of <em>S</em> Panama has revealed important information about the population structure, AMR, global ecology, and genetic markers of invasiveness of the identified genomic subtypes. Our findings provide an important baseline for understanding <em>S</em> Panama infection. The presence of multidrug-resistant clades with elevated invasiveness index values should be monitored through ongoing surveillance, a","PeriodicalId":46633,"journal":{"name":"Lancet Microbe","volume":"6 9","pages":"Article 101150"},"PeriodicalIF":20.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144733875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasmodium interspecies fertilisation: rare or underexplored","authors":"Nimita Deora ,&nbsp;Miles B Markus ,&nbsp;Abhinav Sinha","doi":"10.1016/j.lanmic.2025.101161","DOIUrl":"10.1016/j.lanmic.2025.101161","url":null,"abstract":"","PeriodicalId":46633,"journal":{"name":"Lancet Microbe","volume":"6 9","pages":"Article 101161"},"PeriodicalIF":20.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144235529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing mechanistic understanding of post-acute sequelae of Ebola virus disease","authors":"Xinyue Wang ,&nbsp;Yaxian Kong","doi":"10.1016/j.lanmic.2025.101139","DOIUrl":"10.1016/j.lanmic.2025.101139","url":null,"abstract":"","PeriodicalId":46633,"journal":{"name":"Lancet Microbe","volume":"6 9","pages":"Article 101139"},"PeriodicalIF":20.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144050119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From parasite to partner: unravelling the multifaceted role of Blastocystis in human health and disease","authors":"Lei Deng PhD ,&nbsp;Kevin S W Tan PhD","doi":"10.1016/j.lanmic.2025.101155","DOIUrl":"10.1016/j.lanmic.2025.101155","url":null,"abstract":"<div><div><em>Blastocystis</em>, a common protist in the human gut microbiota, has been identified as a key modulator of gut health and disease progression. Research highlights the involvement of <em>Blastocystis</em> in inflammatory bowel disease, cognitive function, and metabolic health. Despite an increasing interest on this topic, the literature on <em>Blastocystis</em> remains fragmented and often contradictory, with subtype-specific effects inadequately defined and their underlying mechanisms insufficiently elucidated. In this Personal View, we provide an in-depth discussion of the dual roles of <em>Blastocystis</em> subtypes that can either exacerbate gut inflammation by disrupting the epithelial barrier or confer protection by enhancing barrier integrity and inducing anti-inflammatory immune responses. We also examine the effect of <em>Blastocystis</em> on the gut–brain axis, identifying associations between <em>Blastocystis</em> colonisation and cognitive and behavioural changes mediated through alterations in gut microbial composition and metabolite profiles. Moreover, we highlight the association of <em>Blastocystis</em> colonisation with healthier dietary patterns and improved cardiometabolic profiles. These findings could contribute to a comprehensive understanding of <em>Blastocystis</em> and its potential as a biomarker of gut health.</div></div>","PeriodicalId":46633,"journal":{"name":"Lancet Microbe","volume":"6 9","pages":"Article 101155"},"PeriodicalIF":20.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144337147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global emergence and transmission dynamics of carbapenemase-producing Citrobacter freundii sequence type 22 high-risk international clone: a retrospective, genomic, epidemiological study","authors":"Qiaojun Wang BSc, BSc ,&nbsp;Longjie Zhou BSc ,&nbsp;Xiaoling Chen BSc ,&nbsp;Jiayao Yao BSc ,&nbsp;Xinran Sun MSc ,&nbsp;Kai Peng PhD ,&nbsp;Yanyun Gao MSc ,&nbsp;Prof Edward J Feil PhD ,&nbsp;Prof Yunsong Yu PhD ,&nbsp;Prof Zhiqiang Wang PhD ,&nbsp;Prof Ruichao Li PhD ,&nbsp;Prof Xi Li PhD","doi":"10.1016/j.lanmic.2025.101149","DOIUrl":"10.1016/j.lanmic.2025.101149","url":null,"abstract":"<div><h3>Background</h3><div>Carbapenemase-producing <em>Citrobacter</em> (CPC) species have recently been recognised as emerging pathogens associated with nosocomial infections in humans. The increased rate of <em>Citrobacter freundii</em> infections is a public health concern and there is a paucity of genomic data regarding its global transmission dynamics. We aimed to characterise the genetic features of CPC species, and their associated carbapenemase-encoding plasmids, obtained from hospitalised patients in China and from publicly available global data, with a particular focus on high-risk clones.</div></div><div><h3>Methods</h3><div>This was a retrospective, genomic epidemiological study of CPC species obtained from a tertiary hospital in Zhejiang Province, China, from March 5, 2013, to March 5, 2023. We used antimicrobial susceptibility testing, short-read and long-read whole-genome sequencing, phylogenomic analysis, and plasmid structure analysis. A global dataset of complete plasmid sequences encoding <em>bla</em>_KPC, <em>bla</em>_NDM, and <em>bla</em>_IMP was constructed from the National Center for Biotechnology Information (NCBI) RefSeq database to provide insights into their diversity and distribution. All carbapenemase-producing <em>Citrobacter freundii</em> genomes from the NCBI GenBank database were incorporated in the comparative genomic analyses. Bayesian phylogeographical analysis and growth rate assays were carried out to characterise the high-risk <em>C freundii</em> sequence type (ST) 22 clone.</div></div><div><h3>Findings</h3><div>1724 <em>Citrobacter</em> species isolates were collected from diverse clinical specimens, with 48 identified as CPC species. <em>Citrobacter koseri</em> (22 [46%] of 48) and <em>C freundii</em> (20 [42%]) were the predominant CPC species. Comparative analysis found <em>C freundii</em> carried significantly higher median numbers of plasmid replicons (5·0 [IQR 3·3–6·0] <em>vs</em> 2·0 [2·0–3·0]; p&lt;0·0001) and acquired antimicrobial resistance genes (12·0 [7·3–15·8] <em>vs</em> 3·0 [3·0–5·3]; p&lt;0·0001) than did <em>C koseri</em>. Molecular characterisation identified Inc-type plasmids, In<em>823</em>::Kl.pn.I3/In<em>1589</em>-like/In<em>837</em>-like integrons, Tn<em>6296</em>/Tn<em>125</em>/Tn<em>5060</em> transposons, and insertion sequences (eg, IS<em>26</em>, IS<em>3000</em>, IS<em>5</em>, IS<em>Aba125</em>, IS<em>CR1</em>), collectively facilitating the dissemination of carbapenemase genes. Global analysis of 3126 carbapenemase-encoding plasmids found epidemic plasmids with broad host ranges and global diversity. Phylogenetic investigation of predominant carbapenemase-encoding plasmids showed their persistence across geographical regions, temporal spans, and Enterobacterales species, exhibiting high genetic similarity to our clinical plasmids. A phylogenetic tree of 726 global carbapenemase-producing <em>C freundii</em> genomes showed that ST22 (227 [31·3%]) ","PeriodicalId":46633,"journal":{"name":"Lancet Microbe","volume":"6 9","pages":"Article 101149"},"PeriodicalIF":20.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144668662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blastocystis: the ongoing parasitological debate","authors":"The Lancet Microbe","doi":"10.1016/j.lanmic.2025.101232","DOIUrl":"10.1016/j.lanmic.2025.101232","url":null,"abstract":"","PeriodicalId":46633,"journal":{"name":"Lancet Microbe","volume":"6 9","pages":"Article 101232"},"PeriodicalIF":20.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144973801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drug-resistant fungi: the unintended consequence of modern immunosuppression.","authors":"Xiaoqing Fan, Jingyuan Ning","doi":"10.1016/j.lanmic.2025.101225","DOIUrl":"https://doi.org/10.1016/j.lanmic.2025.101225","url":null,"abstract":"","PeriodicalId":46633,"journal":{"name":"Lancet Microbe","volume":" ","pages":"101225"},"PeriodicalIF":20.4,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144973844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Partial deletion of the OPG164 (A36R) transmembrane phosphoprotein in monkeypox virus clade Ib/sh2023 disrupts the active region involving Tyr140.","authors":"Anuj Kumar, Gustavo Sganzerla Martinez, Mansi Dutt, Ali Toloue Ostadgavahi, Alyson Kelvin, David J Kelvin","doi":"10.1016/j.lanmic.2025.101223","DOIUrl":"https://doi.org/10.1016/j.lanmic.2025.101223","url":null,"abstract":"","PeriodicalId":46633,"journal":{"name":"Lancet Microbe","volume":" ","pages":"101223"},"PeriodicalIF":20.4,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144973780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Repopulate, not just decolonise-a microbial ecological strategy against multidrug resistance in intensive care units.","authors":"Mohamad-Hani Temsah, Jaffar A Al-Tawfiq","doi":"10.1016/j.lanmic.2025.101206","DOIUrl":"https://doi.org/10.1016/j.lanmic.2025.101206","url":null,"abstract":"","PeriodicalId":46633,"journal":{"name":"Lancet Microbe","volume":" ","pages":"101206"},"PeriodicalIF":20.4,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144973782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbapenemase-producing Citrobacter species in Chinese children, 2015-24: emerging cefiderocol-resistant hypervirulent clones.","authors":"Chen Xu, Lili Huang, Xiangkun Zeng, Ruichao Li, Ning Dong","doi":"10.1016/j.lanmic.2025.101221","DOIUrl":"https://doi.org/10.1016/j.lanmic.2025.101221","url":null,"abstract":"","PeriodicalId":46633,"journal":{"name":"Lancet Microbe","volume":" ","pages":"101221"},"PeriodicalIF":20.4,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144973787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}